pycom111g02070

Altered inheritance of mitochondria protein 32-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0001205
Physical Location & Seq
Reverse (-)
1242240 .. 1245042
2803 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g02070.4

Sequence Viewer

Length: 1104 bp
ATGGCCTCCGACGACCTCTCTGCCGCCGTCACCGCCGCCGAAGACGAGAAGTTCGGCTTCAAGAGATCGGAGATGTTCAAGGAAAAACTTGCCGGTACGGTCAACGCCTACGACCGCCACGTCTTCCTCTGCTACAAGACCCCCGAGGCCTGGCCGTCGCGCGTCGAAGGCTCTGAGTCCGATCCACTACCTAAGCTCTTCGCCTCCGCTCTCAAAGCTCGCAAGAACGACATCGCCGTTAAGACGTTGCTGACAGTAATTGAAGGACGCGAAGGAACTGAGTTTTCCGATGGCGATGTATTGATTTTCCCACAAATGATCAAATACAGGGGCTTGAAAGAGTCGGATGTGGACAGCTTTGTTGATGATGTCCTTGTGAATGACAAACCGTGGGCTTCCGGAGTGCAAGAGGCATTGACTGGCTCCCATGTATTTGTATGTGCACATGGTAGTCGAGATAAAAGATGTGGTGTTTGTGGGCCTGTTCTCATTGATAAGTTCAAAGATGAGGCTGAGCTGCGAGGATTGACGAATCAGGTATTTGTTACTGCTTGCTCTCACATTGGAGGCCACAAGTATGCTGGAAACTTGATTATCTACAGCCCAGGTTCAGATGGAAGCATAACAGGCCATTGGTATGGCTATGTCACTCCTGATGATGTGCCAGAATTGCTTGATCAGCATATTGGGAAGGGAGAGATCATCGAACGACTTTGGAGGGGCCAAATGGGAGTATCTTCTGATGAAGCTGAAAAAATTAATGACCCGAAGCTTTCAAATGGAGGAGAAAGTAAGAAAATCCAGGAGAAGCCACAAGAAAACGGCAATCAGATACAGAAAAATGAGAACTTTTCAGGCTGTTGCCAAGGTGCTAATAGTAATGGATTTACATGCTGCAAAGAAGTAAGTTTGGAGCAGAATGGTGGAAGTGAGAAAAAGAAGCTGAAAGAAACCAGAGAGTCGTGTGCCAGGAAGGATGCCTTGGGGAAGCTGTCAAGCTTGATTGGGAACTGGGAGCAAAGCGATGTTCTTGCAGCTGCTGCTGTGGTTGGAGCAGTGGCAACAGTGGCCATGGCCTATAGCTTTTATAGAAGGTCAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Suc_Fer-like

Protein Analysis

368

Amino Acids

39.96

Weight (kDa)

5.54

Isoelectric Point (pI)

29.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AccBSI CCGCTC 1 cut(s) 209
AccII CGCG 3 cut(s) 160, 162, 270
AccIII TCCGGA 1 cut(s) 398
AciI CCGC 5 cut(s) 24, 33, 36, 115, 207
AclWI GGATC 1 cut(s) 176
AcoI YGGCCR 2 cut(s) 152, 1068
AfaI GTAC 1 cut(s) 97
AfiI CCNNNNNNNGG 1 cut(s) 150
AgsI TTSAA 6 cut(s) 61, 79, 263, 337, 502, 777
AjiI CACGTC 1 cut(s) 121
AjnI CCWGG 4 cut(s) 149, 604, 801, 968
AjuI GAANNNNNNNTTGG 2 cut(s) 965, 997
Alw21I GWGCWC 1 cut(s) 445
Alw44I GTGCAC 1 cut(s) 441
AlwI GGATC 1 cut(s) 176
AlwNI CAGNNNCTG 1 cut(s) 1040
Ama87I CYCGRG 1 cut(s) 143
Aor13HI TCCGGA 1 cut(s) 398
AoxI GGCC 9 cut(s) 3, 147, 152, 479, 568, 628, 721, 1068, 1074
ApaLI GTGCAC 1 cut(s) 441
ApeKI GCWGC 5 cut(s) 517, 894, 1034, 1037, 1040
ArsI GACNNNNNNTTYG 2 cut(s) 35, 67
AseI ATTAAT 1 cut(s) 759
AspLEI GCGC 1 cut(s) 162
AspS9I GGNCC 2 cut(s) 479, 721
AsuHPI GGTGA 1 cut(s) 22
AvaI CYCGRG 1 cut(s) 143
BaeGI GKGCMC 1 cut(s) 445
BalI TGGCCA 1 cut(s) 1070
BbsI GAAGAC 2 cut(s) 48, 115
Bbv12I GWGCWC 1 cut(s) 445
BbvI GCAGC 5 cut(s) 504, 881, 1024, 1027, 1046
BccI CCATC 2 cut(s) 284, 608
BceAI ACGGC 4 cut(s) 11, 139, 221, 838
BcgI CGANNNNNNTGC 3 cut(s) 36, 1013, 1047
BciT130I CCWGG 4 cut(s) 151, 606, 803, 970
BclI TGATCA 2 cut(s) 318, 676
BfmI CTRYAG 2 cut(s) 598, 1078
BisI GCNGC 7 cut(s) 24, 36, 518, 895, 1035, 1038, 1041
BlpI GCTNAGC 1 cut(s) 513
BlsI GCNGC 7 cut(s) 25, 37, 519, 896, 1036, 1039, 1042
Bme1390I CCNGG 4 cut(s) 151, 606, 803, 970
BmeT110I CYCGRG 1 cut(s) 143
BmgBI CACGTC 1 cut(s) 121
BmgT120I GGNCC 2 cut(s) 479, 721
BmiI GGNNCC 2 cut(s) 424, 722
BmrFI CCNGG 4 cut(s) 151, 606, 803, 970
BmrI ACTGGG 1 cut(s) 1021
BmsI GCATC 1 cut(s) 967
BmuI ACTGGG 1 cut(s) 1021
BpiI GAAGAC 2 cut(s) 48, 115
Bpu10I CCTNAGC 1 cut(s) 192
Bpu1102I GCTNAGC 1 cut(s) 513
BsaJI CCNNGG 6 cut(s) 144, 389, 604, 865, 981, 1071
BsaWI WCCGGW 1 cut(s) 398
Bsc4I CCNNNNNNNGG 1 cut(s) 150
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 2 cut(s) 424, 1016
BseAI TCCGGA 1 cut(s) 398
BseBI CCWGG 4 cut(s) 151, 606, 803, 970
BseDI CCNNGG 6 cut(s) 144, 389, 604, 865, 981, 1071
BseGI GGATG 2 cut(s) 352, 982
BseLI CCNNNNNNNGG 1 cut(s) 150
BseMII CTCAG 3 cut(s) 165, 270, 504
BseNI ACTGG 2 cut(s) 424, 1016
BseRI GAGGAG 1 cut(s) 798
BseSI GKGCMC 1 cut(s) 445
BseXI GCAGC 5 cut(s) 504, 881, 1024, 1027, 1046
Bsh1236I CGCG 3 cut(s) 160, 162, 270
Bsh1285I CGRYCG 1 cut(s) 115
BshFI GGCC 9 cut(s) 5, 149, 154, 481, 570, 630, 723, 1070, 1076
BsiEI CGRYCG 1 cut(s) 115
BsiHKAI GWGCWC 1 cut(s) 445
BsiHKCI CYCGRG 1 cut(s) 143
BsiSI CCGG 2 cut(s) 93, 399
BslI CCNNNNNNNGG 1 cut(s) 150
BsnI GGCC 9 cut(s) 5, 149, 154, 481, 570, 630, 723, 1070, 1076
BsoBI CYCGRG 1 cut(s) 143
Bsp1286I GDGCHC 1 cut(s) 445
Bsp13I TCCGGA 1 cut(s) 398
Bsp143I GATC 5 cut(s) 65, 181, 318, 676, 699
Bsp1720I GCTNAGC 1 cut(s) 513
Bsp19I CCATGG 1 cut(s) 1071
BspACI CCGC 5 cut(s) 24, 33, 36, 115, 207
BspANI GGCC 9 cut(s) 5, 149, 154, 481, 570, 630, 723, 1070, 1076
BspCNI CTCAG 3 cut(s) 166, 271, 505
BspEI TCCGGA 1 cut(s) 398
BspFNI CGCG 3 cut(s) 160, 162, 270
BspLI GGNNCC 2 cut(s) 424, 722
BspPI GGATC 1 cut(s) 176
BspQI GCTCTTC 1 cut(s) 203
BsrBI CCGCTC 1 cut(s) 209
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 2 cut(s) 424, 1016
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 6 cut(s) 144, 389, 604, 865, 981, 1071
BssMI GATC 5 cut(s) 65, 181, 318, 676, 699
BssT1I CCWWGG 3 cut(s) 865, 981, 1071
Bst2UI CCWGG 4 cut(s) 151, 606, 803, 970
Bst4CI ACNGT 4 cut(s) 100, 256, 390, 1066
Bst6I CTCTTC 1 cut(s) 203
BstAPI GCANNNNNTGC 1 cut(s) 1040
BstC8I GCNNGC 2 cut(s) 220, 553
BstDEI CTNAG 4 cut(s) 174, 192, 279, 513
BstDSI CCRYGG 2 cut(s) 389, 1071
BstF5I GGATG 2 cut(s) 352, 982
BstFNI CGCG 3 cut(s) 160, 162, 270
BstHHI GCGC 1 cut(s) 162
BstKTI GATC 5 cut(s) 68, 184, 321, 679, 702
BstMBI GATC 5 cut(s) 65, 181, 318, 676, 699
BstMCI CGRYCG 1 cut(s) 115
BstMWI GCNNNNNNNGC 8 cut(s) 32, 168, 215, 627, 670, 679, 1040, 1067
BstNI CCWGG 4 cut(s) 151, 606, 803, 970
BstNSI RCATGY 1 cut(s) 894
BstSCI CCNGG 4 cut(s) 149, 604, 801, 968
BstSFI CTRYAG 2 cut(s) 598, 1078
BstSLI GKGCMC 1 cut(s) 445
BstUI CGCG 3 cut(s) 160, 162, 270
BstV1I GCAGC 5 cut(s) 504, 881, 1024, 1027, 1046
BstV2I GAAGAC 2 cut(s) 48, 115
BstXI CCANNNNNNTGG 1 cut(s) 638
BsuRI GGCC 9 cut(s) 5, 149, 154, 481, 570, 630, 723, 1070, 1076
BtgI CCRYGG 2 cut(s) 389, 1071
BtgZI GCGATG 3 cut(s) 217, 309, 1038
BtrI CACGTC 1 cut(s) 121
BtsCI GGATG 2 cut(s) 352, 982
BtsI GCAGTG 1 cut(s) 1062
BtsIMutI CAGTG 2 cut(s) 1062, 1071
Cac8I GCNNGC 2 cut(s) 220, 553
CaiI CAGNNNCTG 1 cut(s) 1040
CfoI GCGC 1 cut(s) 162
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 2 cut(s) 479, 721
CseI GACGC 2 cut(s) 151, 276
Csp6I GTAC 1 cut(s) 96
CviAII CATG 4 cut(s) 428, 446, 891, 1072
CviQI GTAC 1 cut(s) 96
DdeI CTNAG 4 cut(s) 174, 192, 279, 513
DpnI GATC 5 cut(s) 67, 183, 320, 678, 701
DpnII GATC 5 cut(s) 65, 181, 318, 676, 699
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 2 cut(s) 152, 1068
Eam1104I CTCTTC 1 cut(s) 203
EarI CTCTTC 1 cut(s) 203
Eco130I CCWWGG 3 cut(s) 865, 981, 1071
Eco147I AGGCCT 1 cut(s) 149
Eco88I CYCGRG 1 cut(s) 143
EcoRII CCWGG 4 cut(s) 149, 604, 801, 968
EcoT14I CCWWGG 3 cut(s) 865, 981, 1071
ErhI CCWWGG 3 cut(s) 865, 981, 1071
FaeI CATG 4 cut(s) 431, 449, 894, 1075
FalI AAGNNNNNCTT 4 cut(s) 41, 73, 965, 997
FatI CATG 4 cut(s) 427, 445, 890, 1071
FbaI TGATCA 2 cut(s) 318, 676
Fnu4HI GCNGC 7 cut(s) 24, 36, 518, 895, 1035, 1038, 1041
FokI GGATG 2 cut(s) 359, 989
Fsp4HI GCNGC 7 cut(s) 24, 36, 518, 895, 1035, 1038, 1041
GlaI GCGC 1 cut(s) 161
GluI GCNGC 7 cut(s) 24, 36, 518, 895, 1035, 1038, 1041
HaeIII GGCC 9 cut(s) 5, 149, 154, 481, 570, 630, 723, 1070, 1076
HapII CCGG 2 cut(s) 93, 399
HgaI GACGC 2 cut(s) 151, 276
HhaI GCGC 1 cut(s) 162
Hin1II CATG 4 cut(s) 431, 449, 894, 1075
Hin6I GCGC 1 cut(s) 160
HinP1I GCGC 1 cut(s) 160
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HindIII AAGCTT 2 cut(s) 770, 997
HinfI GANTC 4 cut(s) 176, 341, 532, 959
HpaII CCGG 2 cut(s) 93, 399
HphI GGTGA 1 cut(s) 22
Hpy166II GTNNAC 3 cut(s) 103, 352, 443
Hpy188I TCNGA 9 cut(s) 10, 70, 175, 181, 289, 346, 613, 742, 831
Hpy188III TCNNGA 4 cut(s) 61, 399, 455, 653
Hpy8I GTNNAC 3 cut(s) 103, 352, 443
Hpy99I CGWCG 3 cut(s) 14, 160, 167
HpyAV CCTTC 6 cut(s) 161, 257, 266, 685, 967, 1086
HpyCH4III ACNGT 4 cut(s) 100, 256, 390, 1066
HpyCH4IV ACGT 2 cut(s) 120, 245
HpyCH4V TGCA 4 cut(s) 406, 443, 897, 1034
HpyF10VI GCNNNNNNNGC 8 cut(s) 32, 168, 215, 627, 670, 679, 1040, 1067
HpyF3I CTNAG 4 cut(s) 174, 192, 279, 513
HpySE526I ACGT 2 cut(s) 120, 245
Hsp92II CATG 4 cut(s) 431, 449, 894, 1075
HspAI GCGC 1 cut(s) 160
Kpn2I TCCGGA 1 cut(s) 398
Ksp22I TGATCA 2 cut(s) 318, 676
Kzo9I GATC 5 cut(s) 65, 181, 318, 676, 699
LguI GCTCTTC 1 cut(s) 203
LmnI GCTCC 4 cut(s) 428, 913, 1015, 1052
Lsp1109I GCAGC 5 cut(s) 504, 881, 1024, 1027, 1046
LweI GCATC 1 cut(s) 967
MaeII ACGT 2 cut(s) 120, 245
MaeIII GTNAC 3 cut(s) 28, 544, 646
MalI GATC 5 cut(s) 67, 183, 320, 678, 701
MbiI CCGCTC 1 cut(s) 209
MboI GATC 5 cut(s) 65, 181, 318, 676, 699
MboII GAAGA 4 cut(s) 53, 115, 190, 729
MhlI GDGCHC 1 cut(s) 445
MlsI TGGCCA 1 cut(s) 1070
MluCI AATT 3 cut(s) 258, 668, 756
MluNI TGGCCA 1 cut(s) 1070
MlyI GAGTC 3 cut(s) 185, 350, 968
MmeI TCCRAC 3 cut(s) 33, 324, 1030
Mox20I TGGCCA 1 cut(s) 1070
MroI TCCGGA 1 cut(s) 398
MscI TGGCCA 1 cut(s) 1070
MseI TTAA 2 cut(s) 240, 759
MslI CAYNNNNRTG 2 cut(s) 576, 636
Msp20I TGGCCA 1 cut(s) 1070
MspA1I CMGCKG 1 cut(s) 1037
MspI CCGG 2 cut(s) 93, 399
MspR9I CCNGG 4 cut(s) 151, 606, 803, 970
MvaI CCWGG 4 cut(s) 151, 606, 803, 970
MvnI CGCG 3 cut(s) 160, 162, 270
MwoI GCNNNNNNNGC 8 cut(s) 32, 168, 215, 627, 670, 679, 1040, 1067
NcoI CCATGG 1 cut(s) 1071
NdeII GATC 5 cut(s) 65, 181, 318, 676, 699
NlaIII CATG 4 cut(s) 431, 449, 894, 1075
NlaIV GGNNCC 2 cut(s) 424, 722
NmuCI GTSAC 2 cut(s) 28, 646
NspI RCATGY 1 cut(s) 894
PceI AGGCCT 1 cut(s) 149
PciSI GCTCTTC 1 cut(s) 203
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 234
PfeI GAWTC 1 cut(s) 532
PfoI TCCNGGA 1 cut(s) 801
PkrI GCNGC 7 cut(s) 25, 37, 519, 896, 1036, 1039, 1042
PleI GAGTC 3 cut(s) 184, 349, 967
PpsI GAGTC 3 cut(s) 184, 349, 967
PshBI ATTAAT 1 cut(s) 759
Psp6I CCWGG 4 cut(s) 149, 604, 801, 968
PspGI CCWGG 4 cut(s) 149, 604, 801, 968
PspN4I GGNNCC 2 cut(s) 424, 722
PspPI GGNCC 2 cut(s) 479, 721
PstNI CAGNNNCTG 1 cut(s) 1040
PvuII CAGCTG 1 cut(s) 1037
RsaI GTAC 1 cut(s) 97
RsaNI GTAC 1 cut(s) 96
RseI CAYNNNNRTG 2 cut(s) 576, 636
SapI GCTCTTC 1 cut(s) 203
SaqAI TTAA 2 cut(s) 240, 759
SatI GCNGC 7 cut(s) 24, 36, 518, 895, 1035, 1038, 1041
Sau3AI GATC 5 cut(s) 65, 181, 318, 676, 699
Sau96I GGNCC 2 cut(s) 479, 721
SchI GAGTC 3 cut(s) 185, 350, 968
ScrFI CCNGG 4 cut(s) 151, 606, 803, 970
SduI GDGCHC 1 cut(s) 445
SfaNI GCATC 1 cut(s) 967
SfcI CTRYAG 2 cut(s) 598, 1078
SmiMI CAYNNNNRTG 2 cut(s) 576, 636
Sse9I AATT 3 cut(s) 258, 668, 756
SseBI AGGCCT 1 cut(s) 149
SsiI CCGC 5 cut(s) 24, 33, 36, 115, 207
StuI AGGCCT 1 cut(s) 149
StyD4I CCNGG 4 cut(s) 149, 604, 801, 968
StyI CCWWGG 3 cut(s) 865, 981, 1071
TaaI ACNGT 4 cut(s) 100, 256, 390, 1066
TaiI ACGT 2 cut(s) 123, 248
TaqI TCGA 3 cut(s) 165, 454, 705
TasI AATT 3 cut(s) 258, 668, 756
TauI GCSGC 2 cut(s) 26, 38
TfiI GAWTC 1 cut(s) 532
Tru1I TTAA 2 cut(s) 240, 759
Tru9I TTAA 2 cut(s) 240, 759
TscAI CASTG 2 cut(s) 1062, 1071
TseFI GTSAC 2 cut(s) 28, 646
TseI GCWGC 5 cut(s) 517, 894, 1034, 1037, 1040
Tsp45I GTSAC 2 cut(s) 28, 646
TspDTI ATGAA 1 cut(s) 759
TspRI CASTG 2 cut(s) 1062, 1071
VneI GTGCAC 1 cut(s) 441
VspI ATTAAT 1 cut(s) 759
XceI RCATGY 1 cut(s) 894
XcmI CCANNNNNNNNNTGG 1 cut(s) 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.