MD09G1045300.v1.1

Altered inheritance of mitochondria protein 32-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001205
Physical Location & Seq
Forward (+)
2952527 .. 2954012
1486 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1045300.v1.1.491

Sequence Viewer

Length: 843 bp
ATGGCTCAGTTTACGATTAATTCCTTGCCTTACAAGGAATTAAAGAGTGTTAGGGGCTTGAAAGAGTCGGATGTGGATAGCTTTGTTGATGATGTCATTGTGAATGACAAACCATGGGCTTCCGGAGTGCAAAAGCCGTTGATTGGCCCCCGTGTATTTATATGCGCACATATGTGTGAACATCCTTTTTATCGTTTGGCCGCATCTATTCTCATTGATGATTTCAAACGGGAGACTAAGTTGCGAGGATTGACGGATCAGGTATTTGTTACTGCTTGCTTTCAAATTGAAGGCCAAAAGTATGCTAGAGAACTCAGGATCTACAGCCCAGATTCAGATGGAAGAATGATATACCATTGGTATCCCTTCGTCACCTTTTCTGCCGTGTCGCAATTGCTTGATAAGCATATTGGGAAGGGAGAGATTATAGAACGACATTGGAGGGGAGCATCTTCTGATAAAGCTGAAGAAATTAATGACCGGGAGCTTCCAAATGGAGAAGAAAATAAGAAAATTGAGGAGAAGCCACAAGAAAATGGCAATCAGATTCACCAAGAAAAGGGCAATCAGATTGAGAATAATGAGAACTTTTCAGGCTATTGCCAGGGTGCTAATAGTGATGGATTTACATGTTGTGAAGAAGTAAGTTTGGAGGAGAACGGTGGAAGTGAGGAAAAAAAGCCGAAAGAAACCAGAGAGTCGTGTGCTAGAAAGGATGGCTTACGGAAGCTGTCAAGCTTGATTGGGAACTGGGAGCAAAGCGATGTTCTCGCAGCTACTGTCGTGGTTGGAGCGGTGGCAACAGTGGTTGTGGCCTATAACTTTTACAAAAGGTCAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Suc_Fer-like

Protein Analysis

281

Amino Acids

31.62

Weight (kDa)

5.43

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Suc_Fer-like PF06999 11 - 149 2e-09 Sucrase/ferredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 166
AccBSI CCGCTC 1 cut(s) 794
AccIII TCCGGA 1 cut(s) 122
AciI CCGC 2 cut(s) 201, 794
AclWI GGATC 2 cut(s) 264, 326
AcoI YGGCCR 1 cut(s) 198
AcuI CTGAAG 1 cut(s) 486
AfiI CCNNNNNNNGG 2 cut(s) 143, 559
AflIII ACRYGT 1 cut(s) 629
AgsI TTSAA 4 cut(s) 61, 226, 284, 290
AjnI CCWGG 1 cut(s) 603
AleI CACNNNNGTG 1 cut(s) 172
AluBI AGCT 6 cut(s) 81, 464, 487, 730, 738, 776
AluI AGCT 6 cut(s) 81, 464, 487, 730, 738, 776
Alw26I GTCTC 1 cut(s) 227
AlwI GGATC 2 cut(s) 264, 326
AlwNI CAGNNNCTG 1 cut(s) 779
Aor13HI TCCGGA 1 cut(s) 122
AoxI GGCC 4 cut(s) 145, 198, 292, 813
ApeKI GCWGC 1 cut(s) 773
AseI ATTAAT 2 cut(s) 18, 474
AspLEI GCGC 1 cut(s) 167
AspS9I GGNCC 1 cut(s) 146
AsuC2I CCSGG 1 cut(s) 482
AsuHPI GGTGA 2 cut(s) 364, 542
BbvI GCAGC 1 cut(s) 785
BccI CCATC 3 cut(s) 332, 614, 710
BceAI ACGGC 2 cut(s) 121, 368
BciT130I CCWGG 1 cut(s) 605
BciVI GTATCC 1 cut(s) 372
BcnI CCSGG 1 cut(s) 482
BcoDI GTCTC 1 cut(s) 227
BfaI CTAG 2 cut(s) 306, 708
BfmI CTRYAG 1 cut(s) 322
BfuI GTATCC 1 cut(s) 372
BisI GCNGC 2 cut(s) 201, 774
BlsI GCNGC 2 cut(s) 202, 775
Bme1390I CCNGG 2 cut(s) 482, 605
BmgT120I GGNCC 1 cut(s) 146
BmiI GGNNCC 1 cut(s) 148
BmrFI CCNGG 2 cut(s) 482, 605
BmrI ACTGGG 1 cut(s) 760
BmsI GCATC 2 cut(s) 212, 458
BmuI ACTGGG 1 cut(s) 760
BpuMI CCSGG 1 cut(s) 482
BsaJI CCNNGG 2 cut(s) 113, 604
BsaWI WCCGGW 1 cut(s) 122
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 559
Bse1I ACTGG 1 cut(s) 755
BseAI TCCGGA 1 cut(s) 122
BseBI CCWGG 1 cut(s) 605
BseDI CCNNGG 2 cut(s) 113, 604
BseGI GGATG 3 cut(s) 76, 181, 721
BseLI CCNNNNNNNGG 2 cut(s) 143, 559
BseMII CTCAG 2 cut(s) 20, 328
BseNI ACTGG 1 cut(s) 755
BseRI GAGGAG 2 cut(s) 533, 668
BseXI GCAGC 1 cut(s) 785
BshFI GGCC 4 cut(s) 147, 200, 294, 815
BsiSI CCGG 2 cut(s) 123, 481
BslI CCNNNNNNNGG 2 cut(s) 143, 559
BsmAI GTCTC 1 cut(s) 227
BsnI GGCC 4 cut(s) 147, 200, 294, 815
Bsp13I TCCGGA 1 cut(s) 122
Bsp143I GATC 2 cut(s) 256, 318
Bsp19I CCATGG 1 cut(s) 113
BspACI CCGC 2 cut(s) 201, 794
BspANI GGCC 4 cut(s) 147, 200, 294, 815
BspCNI CTCAG 2 cut(s) 19, 327
BspEI TCCGGA 1 cut(s) 122
BspLI GGNNCC 1 cut(s) 148
BspPI GGATC 2 cut(s) 264, 326
BsrBI CCGCTC 1 cut(s) 794
BsrI ACTGG 1 cut(s) 755
BssECI CCNNGG 2 cut(s) 113, 604
BssMI GATC 2 cut(s) 256, 318
BssT1I CCWWGG 1 cut(s) 113
Bst2UI CCWGG 1 cut(s) 605
Bst4CI ACNGT 3 cut(s) 662, 781, 805
BstC8I GCNNGC 1 cut(s) 277
BstDEI CTNAG 3 cut(s) 6, 237, 314
BstDSI CCRYGG 1 cut(s) 113
BstF5I GGATG 3 cut(s) 76, 181, 721
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 2 cut(s) 259, 321
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 2 cut(s) 256, 318
BstMWI GCNNNNNNNGC 1 cut(s) 403
BstNI CCWGG 1 cut(s) 605
BstNSI RCATGY 1 cut(s) 633
BstSCI CCNGG 2 cut(s) 480, 603
BstSFI CTRYAG 1 cut(s) 322
BstV1I GCAGC 1 cut(s) 785
BstX2I RGATCY 1 cut(s) 318
BstYI RGATCY 1 cut(s) 318
BsuI GTATCC 1 cut(s) 372
BsuRI GGCC 4 cut(s) 147, 200, 294, 815
BtgI CCRYGG 1 cut(s) 113
BtgZI GCGATG 1 cut(s) 777
BtsCI GGATG 3 cut(s) 76, 181, 721
BtsIMutI CAGTG 1 cut(s) 810
Cac8I GCNNGC 1 cut(s) 277
CaiI CAGNNNCTG 1 cut(s) 779
CfoI GCGC 1 cut(s) 167
Cfr13I GGNCC 1 cut(s) 146
CviAII CATG 2 cut(s) 114, 630
DdeI CTNAG 3 cut(s) 6, 237, 314
DpnI GATC 2 cut(s) 258, 320
DpnII GATC 2 cut(s) 256, 318
EaeI YGGCCR 1 cut(s) 198
Eco130I CCWWGG 1 cut(s) 113
Eco57I CTGAAG 1 cut(s) 486
EcoRII CCWGG 1 cut(s) 603
EcoT14I CCWWGG 1 cut(s) 113
ErhI CCWWGG 1 cut(s) 113
FaeI CATG 2 cut(s) 117, 633
FalI AAGNNNNNCTT 2 cut(s) 704, 736
FatI CATG 2 cut(s) 113, 629
FauNDI CATATG 1 cut(s) 171
Fnu4HI GCNGC 2 cut(s) 201, 774
FokI GGATG 3 cut(s) 83, 168, 728
Fsp4HI GCNGC 2 cut(s) 201, 774
FspAI RTGCGCAY 1 cut(s) 166
FspBI CTAG 2 cut(s) 306, 708
FspI TGCGCA 1 cut(s) 166
GlaI GCGC 1 cut(s) 166
GluI GCNGC 2 cut(s) 201, 774
HaeIII GGCC 4 cut(s) 147, 200, 294, 815
HapII CCGG 2 cut(s) 123, 481
HhaI GCGC 1 cut(s) 167
Hin1II CATG 2 cut(s) 117, 633
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HindIII AAGCTT 1 cut(s) 736
HinfI GANTC 4 cut(s) 65, 332, 547, 698
HpaII CCGG 2 cut(s) 123, 481
HphI GGTGA 2 cut(s) 364, 542
Hpy166II GTNNAC 2 cut(s) 12, 179
Hpy188I TCNGA 5 cut(s) 70, 337, 457, 546, 570
Hpy188III TCNNGA 2 cut(s) 123, 316
Hpy8I GTNNAC 2 cut(s) 12, 179
HpyAV CCTTC 3 cut(s) 284, 376, 409
HpyCH4III ACNGT 3 cut(s) 662, 781, 805
HpyCH4V TGCA 1 cut(s) 130
HpyF10VI GCNNNNNNNGC 1 cut(s) 403
HpyF3I CTNAG 3 cut(s) 6, 237, 314
Hsp92II CATG 2 cut(s) 117, 633
HspAI GCGC 1 cut(s) 165
Kpn2I TCCGGA 1 cut(s) 122
Kzo9I GATC 2 cut(s) 256, 318
LmnI GCTCC 4 cut(s) 446, 484, 754, 791
Lsp1109I GCAGC 1 cut(s) 785
LweI GCATC 2 cut(s) 212, 458
MaeI CTAG 2 cut(s) 306, 708
MaeIII GTNAC 2 cut(s) 268, 370
MalI GATC 2 cut(s) 258, 320
MbiI CCGCTC 1 cut(s) 794
MboI GATC 2 cut(s) 256, 318
MboII GAAGA 5 cut(s) 354, 444, 479, 512, 650
MfeI CAATTG 1 cut(s) 392
MflI RGATCY 1 cut(s) 318
MluCI AATT 6 cut(s) 19, 38, 285, 392, 471, 513
MlyI GAGTC 2 cut(s) 74, 707
MmeI TCCRAC 2 cut(s) 48, 769
MnlI CCTC 5 cut(s) 239, 435, 511, 646, 664
MroI TCCGGA 1 cut(s) 122
MseI TTAA 3 cut(s) 18, 41, 474
MslI CAYNNNNRTG 2 cut(s) 172, 174
MspI CCGG 2 cut(s) 123, 481
MspR9I CCNGG 2 cut(s) 482, 605
MunI CAATTG 1 cut(s) 392
MvaI CCWGG 1 cut(s) 605
MwoI GCNNNNNNNGC 1 cut(s) 403
NciI CCSGG 1 cut(s) 482
NcoI CCATGG 1 cut(s) 113
NdeI CATATG 1 cut(s) 171
NdeII GATC 2 cut(s) 256, 318
NlaIII CATG 2 cut(s) 117, 633
NlaIV GGNNCC 1 cut(s) 148
NmuCI GTSAC 1 cut(s) 370
NsbI TGCGCA 1 cut(s) 166
NspI RCATGY 1 cut(s) 633
OliI CACNNNNGTG 1 cut(s) 172
PciI ACATGT 1 cut(s) 629
PfeI GAWTC 2 cut(s) 332, 547
PkrI GCNGC 2 cut(s) 202, 775
PleI GAGTC 2 cut(s) 73, 706
PpsI GAGTC 2 cut(s) 73, 706
PscI ACATGT 1 cut(s) 629
PshBI ATTAAT 2 cut(s) 18, 474
Psp6I CCWGG 1 cut(s) 603
PspGI CCWGG 1 cut(s) 603
PspN4I GGNNCC 1 cut(s) 148
PspPI GGNCC 1 cut(s) 146
PstNI CAGNNNCTG 1 cut(s) 779
PsuI RGATCY 1 cut(s) 318
RseI CAYNNNNRTG 2 cut(s) 172, 174
SaqAI TTAA 3 cut(s) 18, 41, 474
SatI GCNGC 2 cut(s) 201, 774
Sau3AI GATC 2 cut(s) 256, 318
Sau96I GGNCC 1 cut(s) 146
SchI GAGTC 2 cut(s) 74, 707
ScrFI CCNGG 2 cut(s) 482, 605
SetI ASST 9 cut(s) 83, 264, 377, 466, 489, 732, 740, 778, 836
SfaNI GCATC 2 cut(s) 212, 458
SfcI CTRYAG 1 cut(s) 322
SmiMI CAYNNNNRTG 2 cut(s) 172, 174
Sse9I AATT 6 cut(s) 19, 38, 285, 392, 471, 513
SsiI CCGC 2 cut(s) 201, 794
SspMI CTAG 2 cut(s) 306, 708
StyD4I CCNGG 2 cut(s) 480, 603
StyI CCWWGG 1 cut(s) 113
TaaI ACNGT 3 cut(s) 662, 781, 805
TasI AATT 6 cut(s) 19, 38, 285, 392, 471, 513
TauI GCSGC 1 cut(s) 203
TfiI GAWTC 2 cut(s) 332, 547
Tru1I TTAA 3 cut(s) 18, 41, 474
Tru9I TTAA 3 cut(s) 18, 41, 474
TscAI CASTG 1 cut(s) 810
TseFI GTSAC 1 cut(s) 370
TseI GCWGC 1 cut(s) 773
Tsp45I GTSAC 1 cut(s) 370
TspGWI ACGGA 2 cut(s) 269, 739
TspRI CASTG 1 cut(s) 810
VspI ATTAAT 2 cut(s) 18, 474
XceI RCATGY 1 cut(s) 633
XspI CTAG 2 cut(s) 306, 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.