MD09G1024300.v1.1

Altered inheritance of mitochondria protein 32-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001205
Physical Location & Seq
Reverse (-)
1465424 .. 1467237
1814 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1024300.v1.1.491

Sequence Viewer

Length: 318 bp
ATGGAAGCTGAGCTGCGAGGATTGACAAATCAGGTATTTGTTACTGCTTGCTCTCACATTGGAGACCACAAGTGTGCTGGAAACTTGATTGTCTACAGCCCAGGTTCAGATGGAAGCATAACAGGCCATTGGTATGGCTACGTCACTCCTAAGGATGTGCCGGAATTGCTTGATCAGCATATTGGGAAGGGAGAGATCATAGAACGACTTTGGAGGATTAGGGGCCAAATGGGAGCATCTTCGGATGAAGCTGAAAAAATTAATGACCAGAAGCTTCCAAATGGAGGAGAAAGTAAGAAAATGGAGGAGAAGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Suc_Fer-like

Protein Analysis

106

Amino Acids

11.57

Weight (kDa)

5.5

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Suc_Fer-like PF06999 9 - 72 1e-16 Sucrase/ferredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 93
AfiI CCNNNNNNNGG 1 cut(s) 284
AjnI CCWGG 1 cut(s) 100
AleI CACNNNNGTG 1 cut(s) 72
AluBI AGCT 4 cut(s) 8, 13, 251, 274
AluI AGCT 4 cut(s) 8, 13, 251, 274
Alw26I GTCTC 1 cut(s) 57
AoxI GGCC 2 cut(s) 124, 223
ApeKI GCWGC 1 cut(s) 13
AseI ATTAAT 1 cut(s) 261
AspS9I GGNCC 1 cut(s) 223
AxyI CCTNAGG 1 cut(s) 150
BccI CCATC 1 cut(s) 104
BciT130I CCWGG 1 cut(s) 102
BclI TGATCA 1 cut(s) 172
BcoDI GTCTC 1 cut(s) 57
BfmI CTRYAG 1 cut(s) 94
BisI GCNGC 1 cut(s) 14
BlpI GCTNAGC 1 cut(s) 9
BlsI GCNGC 1 cut(s) 15
Bme1390I CCNGG 1 cut(s) 102
BmgT120I GGNCC 1 cut(s) 223
BmiI GGNNCC 1 cut(s) 224
BmrFI CCNGG 1 cut(s) 102
BmsI GCATC 1 cut(s) 245
Bpu1102I GCTNAGC 1 cut(s) 9
BsaI GGTCTC 1 cut(s) 57
BsaJI CCNNGG 1 cut(s) 100
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse21I CCTNAGG 1 cut(s) 150
BseBI CCWGG 1 cut(s) 102
BseDI CCNNGG 1 cut(s) 100
BseGI GGATG 2 cut(s) 160, 250
BseLI CCNNNNNNNGG 1 cut(s) 284
BseRI GAGGAG 1 cut(s) 300
BshFI GGCC 2 cut(s) 126, 225
BsiSI CCGG 1 cut(s) 161
BslI CCNNNNNNNGG 1 cut(s) 284
BsmAI GTCTC 1 cut(s) 57
BsnI GGCC 2 cut(s) 126, 225
Bso31I GGTCTC 1 cut(s) 57
Bsp143I GATC 2 cut(s) 172, 195
Bsp1720I GCTNAGC 1 cut(s) 9
BspANI GGCC 2 cut(s) 126, 225
BspLI GGNNCC 1 cut(s) 224
BspTNI GGTCTC 1 cut(s) 57
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 2 cut(s) 172, 195
Bst2UI CCWGG 1 cut(s) 102
BstC8I GCNNGC 1 cut(s) 49
BstDEI CTNAG 2 cut(s) 9, 150
BstF5I GGATG 2 cut(s) 160, 250
BstKTI GATC 2 cut(s) 175, 198
BstMAI GTCTC 1 cut(s) 57
BstMBI GATC 2 cut(s) 172, 195
BstMWI GCNNNNNNNGC 3 cut(s) 123, 166, 175
BstNI CCWGG 1 cut(s) 102
BstSCI CCNGG 1 cut(s) 100
BstSFI CTRYAG 1 cut(s) 94
BstXI CCANNNNNNTGG 1 cut(s) 134
Bsu36I CCTNAGG 1 cut(s) 150
BsuRI GGCC 2 cut(s) 126, 225
BtsCI GGATG 2 cut(s) 160, 250
Cac8I GCNNGC 1 cut(s) 49
Cfr13I GGNCC 1 cut(s) 223
CviJI RGCY 9 cut(s) 8, 13, 99, 126, 138, 225, 251, 274, 313
CviKI_1 RGCY 9 cut(s) 8, 13, 99, 126, 138, 225, 251, 274, 313
DdeI CTNAG 2 cut(s) 9, 150
DpnI GATC 2 cut(s) 174, 197
DpnII GATC 2 cut(s) 172, 195
Eco31I GGTCTC 1 cut(s) 57
Eco81I CCTNAGG 1 cut(s) 150
EcoRII CCWGG 1 cut(s) 100
FaiI YATR 4 cut(s) 119, 135, 180, 200
FbaI TGATCA 1 cut(s) 172
FblI GTMKAC 1 cut(s) 93
Fnu4HI GCNGC 1 cut(s) 14
FokI GGATG 2 cut(s) 167, 257
Fsp4HI GCNGC 1 cut(s) 14
GluI GCNGC 1 cut(s) 14
HaeIII GGCC 2 cut(s) 126, 225
HapII CCGG 1 cut(s) 161
HindIII AAGCTT 1 cut(s) 272
HpaII CCGG 1 cut(s) 161
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 2 cut(s) 109, 244
Hpy8I GTNNAC 1 cut(s) 94
HpyAV CCTTC 1 cut(s) 181
HpyCH4IV ACGT 1 cut(s) 141
HpyF10VI GCNNNNNNNGC 3 cut(s) 123, 166, 175
HpyF3I CTNAG 2 cut(s) 9, 150
HpySE526I ACGT 1 cut(s) 141
Ksp22I TGATCA 1 cut(s) 172
Kzo9I GATC 2 cut(s) 172, 195
LmnI GCTCC 1 cut(s) 233
LpnPI CCDG 7 cut(s) 17, 63, 87, 108, 114, 174, 281
LweI GCATC 1 cut(s) 245
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 2 cut(s) 40, 142
MalI GATC 2 cut(s) 174, 197
MboI GATC 2 cut(s) 172, 195
MboII GAAGA 1 cut(s) 231
MluCI AATT 2 cut(s) 164, 258
MnlI CCTC 4 cut(s) 11, 207, 278, 298
MseI TTAA 1 cut(s) 261
MslI CAYNNNNRTG 2 cut(s) 72, 132
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 1 cut(s) 102
MvaI CCWGG 1 cut(s) 102
MwoI GCNNNNNNNGC 3 cut(s) 123, 166, 175
NdeII GATC 2 cut(s) 172, 195
NlaIV GGNNCC 1 cut(s) 224
NmuCI GTSAC 1 cut(s) 142
OliI CACNNNNGTG 1 cut(s) 72
PkrI GCNGC 1 cut(s) 15
PshBI ATTAAT 1 cut(s) 261
Psp6I CCWGG 1 cut(s) 100
PspGI CCWGG 1 cut(s) 100
PspN4I GGNNCC 1 cut(s) 224
PspPI GGNCC 1 cut(s) 223
RseI CAYNNNNRTG 2 cut(s) 72, 132
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 1 cut(s) 14
Sau3AI GATC 2 cut(s) 172, 195
Sau96I GGNCC 1 cut(s) 223
ScrFI CCNGG 1 cut(s) 102
SetI ASST 7 cut(s) 10, 15, 36, 106, 144, 253, 276
SfaNI GCATC 1 cut(s) 245
SfcI CTRYAG 1 cut(s) 94
SmiMI CAYNNNNRTG 2 cut(s) 72, 132
Sse9I AATT 2 cut(s) 164, 258
StyD4I CCNGG 1 cut(s) 100
TaiI ACGT 1 cut(s) 144
TasI AATT 2 cut(s) 164, 258
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 1 cut(s) 13
Tsp45I GTSAC 1 cut(s) 142
TspDTI ATGAA 1 cut(s) 261
VspI ATTAAT 1 cut(s) 261
XcmI CCANNNNNNNNNTGG 1 cut(s) 74
XmiI GTMKAC 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.