MD09G1026600.v1.1

Altered inheritance of mitochondria protein 32-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001205
Physical Location & Seq
Reverse (-)
1655819 .. 1658705
2887 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1026600.v1.1.491

Sequence Viewer

Length: 1278 bp
ATGCACATTGCGCACCTACACCCATACCGACACCGCCGTTCGCTCCTCAACCTCCTCATTATATCAACTCCAACAACCACACAACAACGCTCCTTCCCTCTCATCTTTTCCCTCTCATCGTTTTCAACTTCAACCCCGGCTAAGCCCCTCCTTCCTCCTCTCCGAGCCCTAGCAATGGCCTCCGACGGCCTCTCTGCCGCCGTCACGGCCGCCGAAGACGAGAAGTTCGGTTTCAAGAGATCGGAGATGTTCAAGGAAAAACTTGCCGGTACCGTCAACGCCTACGACCGCCACGTCTTCCTCTGCTACAAGACCCCCGAGGCCTGGCCGTCGCGCGTCGAAGGCTCTGAGTCCGATCCGCTCCCTAAGTTCTTCGCCTCCGCTCTCAAAGCTCGCAAGAACGACATCGCCGTTAAGACGTTGCTGACAGTAATTGAAGGACGCGAAGGAACTGAGTTTTCCGACGGCGATGTGTTGATTTTCCCACAAATGATCAAATGCAGGGGCTTGAAAGAGTCGGATGTGGACAGCTTTGTTGATGATGTCCTTGTGAATGACAAACCATGGGCTTCCGGAGTGCAAGAGGCGTTGACTGGCTCCCATGTATTTGTATGTGCACATGGTAGTCGAGATAAAAGATGCGGTGTTTGTGGACCTGTTCTCATTGATAAGTTCAAAGAGGAGGCTGAGCTGCGAGGATTGACGAATCAGGTATTTGTTACTGCTTGCTCTCACATTGGAGGCCACAAATATGCTGGAAACTTGATTATCTACAGCCCAGGTTCAGATGGAAGCATAACAGGCCATTGGTATGGCTATGTCACTCCTGATGATGTGCCAGAATTGCTTGATCAGCATATTGGGAAGGGAGAGATCATCGAACGACTTTGGAGGGGCCAAATGGGAGTATCTTCTGATGAAGCTGAAAAAATGAATGACATGAAGCTTTCAAATGGAGGAGAAAGTAAGAAAATCCAGGAGAAGCCCCAAGAAAACGGCAATCAGATTCAGAAAAACGAGAACTTTTCGGGCTGTTGCCAAGGTGCTAATAGTAATGGATTTACATGCTGCAAAGAAGTAAGTTTGGAGCAGAATGGTGGAAGTGAGAAAAAGAAGCTGAAAGAAACCAGAGAGTCGTGTGCCAGGAAGGATGCCTTAGGGAAGCTGTCAAGCTTGATTGGGAACTGGGAACAAAGCGATGTTCTTGCAGCTGCTGCCATGGTTGGAGCAGTGGCAACAGTGGCCGTGGCCTATAGCTTTTATAGAAGGTCAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Suc_Fer-like

Protein Analysis

426

Amino Acids

46.48

Weight (kDa)

6.83

Isoelectric Point (pI)

37.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Suc_Fer-like PF06999 88 - 299 5.5e-48 Sucrase/ferredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 293
Acc16I TGCGCA 1 cut(s) 12
Acc65I GGTACC 1 cut(s) 269
AccB1I GGYRCC 1 cut(s) 269
AccBSI CCGCTC 2 cut(s) 361, 383
AccII CGCG 3 cut(s) 334, 336, 444
AccIII TCCGGA 1 cut(s) 572
AciI CCGC 7 cut(s) 34, 198, 210, 289, 359, 381, 642
AclWI GGATC 1 cut(s) 350
AcoI YGGCCR 3 cut(s) 207, 326, 1242
AfaI GTAC 1 cut(s) 271
AfiI CCNNNNNNNGG 2 cut(s) 175, 324
AgsI TTSAA 8 cut(s) 126, 132, 235, 253, 437, 511, 676, 951
AjiI CACGTC 1 cut(s) 295
AjnI CCWGG 4 cut(s) 323, 778, 975, 1142
Alw21I GWGCWC 1 cut(s) 619
Alw44I GTGCAC 1 cut(s) 615
AlwI GGATC 1 cut(s) 350
AlwNI CAGNNNCTG 1 cut(s) 1214
Ama87I CYCGRG 1 cut(s) 317
Aor13HI TCCGGA 1 cut(s) 572
ApaLI GTGCAC 1 cut(s) 615
ApeKI GCWGC 5 cut(s) 691, 1068, 1208, 1211, 1214
ArsI GACNNNNNNTTYG 2 cut(s) 209, 241
Asp718I GGTACC 1 cut(s) 269
AspLEI GCGC 2 cut(s) 13, 336
AspS9I GGNCC 2 cut(s) 653, 895
AsuC2I CCSGG 1 cut(s) 137
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 1 cut(s) 653
AxyI CCTNAGG 1 cut(s) 1156
BaeGI GKGCMC 1 cut(s) 619
BanI GGYRCC 1 cut(s) 269
BanII GRGCYC 1 cut(s) 169
BbsI GAAGAC 2 cut(s) 222, 289
Bbv12I GWGCWC 1 cut(s) 619
BbvI GCAGC 5 cut(s) 678, 1055, 1198, 1201, 1220
BccI CCATC 1 cut(s) 782
BceAI ACGGC 9 cut(s) 21, 185, 202, 222, 313, 395, 481, 1012, 1229
BcgI CGANNNNNNTGC 2 cut(s) 1187, 1221
BciT130I CCWGG 4 cut(s) 325, 780, 977, 1144
BclI TGATCA 2 cut(s) 492, 850
BcnI CCSGG 1 cut(s) 137
BfaI CTAG 1 cut(s) 170
BfmI CTRYAG 2 cut(s) 772, 1252
BglI GCCNNNNNGGC 1 cut(s) 206
BisI GCNGC 7 cut(s) 198, 210, 692, 1069, 1209, 1212, 1215
BlpI GCTNAGC 2 cut(s) 141, 687
BlsI GCNGC 7 cut(s) 199, 211, 693, 1070, 1210, 1213, 1216
Bme1390I CCNGG 5 cut(s) 137, 325, 780, 977, 1144
Bme18I GGWCC 1 cut(s) 653
BmeT110I CYCGRG 1 cut(s) 317
BmgBI CACGTC 1 cut(s) 295
BmgT120I GGNCC 2 cut(s) 653, 895
BmiI GGNNCC 3 cut(s) 271, 598, 896
BmrFI CCNGG 5 cut(s) 137, 325, 780, 977, 1144
BmrI ACTGGG 1 cut(s) 1195
BmsI GCATC 2 cut(s) 629, 1141
BmuI ACTGGG 1 cut(s) 1195
BpiI GAAGAC 2 cut(s) 222, 289
Bpu1102I GCTNAGC 2 cut(s) 141, 687
BpuMI CCSGG 1 cut(s) 137
BsaJI CCNNGG 7 cut(s) 135, 318, 563, 778, 1039, 1218, 1245
BsaWI WCCGGW 1 cut(s) 572
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 324
Bse118I RCCGGY 1 cut(s) 266
Bse1I ACTGG 2 cut(s) 598, 1190
Bse21I CCTNAGG 1 cut(s) 1156
Bse3DI GCAATG 2 cut(s) 6, 180
BseAI TCCGGA 1 cut(s) 572
BseBI CCWGG 4 cut(s) 325, 780, 977, 1144
BseDI CCNNGG 7 cut(s) 135, 318, 563, 778, 1039, 1218, 1245
BseGI GGATG 2 cut(s) 526, 1156
BseLI CCNNNNNNNGG 2 cut(s) 175, 324
BseMI GCAATG 2 cut(s) 6, 180
BseMII CTCAG 3 cut(s) 339, 444, 678
BseNI ACTGG 2 cut(s) 598, 1190
BseRI GAGGAG 5 cut(s) 35, 44, 147, 695, 972
BseSI GKGCMC 1 cut(s) 619
BseX3I CGGCCG 1 cut(s) 207
BseXI GCAGC 5 cut(s) 678, 1055, 1198, 1201, 1220
Bsh1236I CGCG 3 cut(s) 334, 336, 444
Bsh1285I CGRYCG 2 cut(s) 210, 289
BshNI GGYRCC 1 cut(s) 269
BsiEI CGRYCG 2 cut(s) 210, 289
BsiHKAI GWGCWC 1 cut(s) 619
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 3 cut(s) 137, 267, 573
BslI CCNNNNNNNGG 2 cut(s) 175, 324
BsoBI CYCGRG 1 cut(s) 317
Bsp1286I GDGCHC 2 cut(s) 169, 619
Bsp13I TCCGGA 1 cut(s) 572
Bsp143I GATC 5 cut(s) 239, 355, 492, 850, 873
Bsp1720I GCTNAGC 2 cut(s) 141, 687
Bsp19I CCATGG 2 cut(s) 563, 1218
BspACI CCGC 7 cut(s) 34, 198, 210, 289, 359, 381, 642
BspCNI CTCAG 3 cut(s) 340, 445, 679
BspEI TCCGGA 1 cut(s) 572
BspFNI CGCG 3 cut(s) 334, 336, 444
BspLI GGNNCC 3 cut(s) 271, 598, 896
BspPI GGATC 1 cut(s) 350
BspT107I GGYRCC 1 cut(s) 269
BsrBI CCGCTC 2 cut(s) 361, 383
BsrDI GCAATG 2 cut(s) 6, 180
BsrFI RCCGGY 1 cut(s) 266
BsrI ACTGG 2 cut(s) 598, 1190
BssAI RCCGGY 1 cut(s) 266
BssECI CCNNGG 7 cut(s) 135, 318, 563, 778, 1039, 1218, 1245
BssMI GATC 5 cut(s) 239, 355, 492, 850, 873
BssT1I CCWWGG 3 cut(s) 563, 1039, 1218
Bst2UI CCWGG 4 cut(s) 325, 780, 977, 1144
Bst4CI ACNGT 3 cut(s) 274, 430, 1240
BstAPI GCANNNNNTGC 1 cut(s) 1214
BstC8I GCNNGC 2 cut(s) 394, 727
BstDEI CTNAG 6 cut(s) 141, 348, 366, 453, 687, 1156
BstDSI CCRYGG 3 cut(s) 563, 1218, 1245
BstF5I GGATG 2 cut(s) 526, 1156
BstFNI CGCG 3 cut(s) 334, 336, 444
BstHHI GCGC 2 cut(s) 13, 336
BstKTI GATC 5 cut(s) 242, 358, 495, 853, 876
BstMBI GATC 5 cut(s) 239, 355, 492, 850, 873
BstMCI CGRYCG 2 cut(s) 210, 289
BstMWI GCNNNNNNNGC 9 cut(s) 10, 206, 342, 389, 801, 844, 853, 1214, 1241
BstNI CCWGG 4 cut(s) 325, 780, 977, 1144
BstNSI RCATGY 1 cut(s) 1068
BstSCI CCNGG 5 cut(s) 135, 323, 778, 975, 1142
BstSFI CTRYAG 2 cut(s) 772, 1252
BstSLI GKGCMC 1 cut(s) 619
BstUI CGCG 3 cut(s) 334, 336, 444
BstV1I GCAGC 5 cut(s) 678, 1055, 1198, 1201, 1220
BstV2I GAAGAC 2 cut(s) 222, 289
BstXI CCANNNNNNTGG 1 cut(s) 812
BstZI CGGCCG 1 cut(s) 207
Bsu36I CCTNAGG 1 cut(s) 1156
BtgI CCRYGG 3 cut(s) 563, 1218, 1245
BtgZI GCGATG 3 cut(s) 391, 483, 1212
BtrI CACGTC 1 cut(s) 295
BtsCI GGATG 2 cut(s) 526, 1156
BtsI GCAGTG 1 cut(s) 1236
BtsIMutI CAGTG 2 cut(s) 1236, 1245
Cac8I GCNNGC 2 cut(s) 394, 727
CaiI CAGNNNCTG 1 cut(s) 1214
CfoI GCGC 2 cut(s) 13, 336
Cfr10I RCCGGY 1 cut(s) 266
Cfr13I GGNCC 2 cut(s) 653, 895
CseI GACGC 2 cut(s) 325, 450
Csp6I GTAC 1 cut(s) 270
CviAII CATG 6 cut(s) 564, 602, 620, 940, 1065, 1219
CviQI GTAC 1 cut(s) 270
DdeI CTNAG 6 cut(s) 141, 348, 366, 453, 687, 1156
DpnI GATC 5 cut(s) 241, 357, 494, 852, 875
DpnII GATC 5 cut(s) 239, 355, 492, 850, 873
DrdI GACNNNNNNGTC 1 cut(s) 293
DseDI GACNNNNNNGTC 1 cut(s) 293
EaeI YGGCCR 3 cut(s) 207, 326, 1242
EagI CGGCCG 1 cut(s) 207
EclXI CGGCCG 1 cut(s) 207
Eco130I CCWWGG 3 cut(s) 563, 1039, 1218
Eco147I AGGCCT 1 cut(s) 323
Eco24I GRGCYC 1 cut(s) 169
Eco47I GGWCC 1 cut(s) 653
Eco52I CGGCCG 1 cut(s) 207
Eco81I CCTNAGG 1 cut(s) 1156
Eco88I CYCGRG 1 cut(s) 317
EcoRII CCWGG 4 cut(s) 323, 778, 975, 1142
EcoT14I CCWWGG 3 cut(s) 563, 1039, 1218
EcoT38I GRGCYC 1 cut(s) 169
ErhI CCWWGG 3 cut(s) 563, 1039, 1218
FaeI CATG 6 cut(s) 567, 605, 623, 943, 1068, 1222
FalI AAGNNNNNCTT 2 cut(s) 1139, 1171
FatI CATG 6 cut(s) 563, 601, 619, 939, 1064, 1218
FbaI TGATCA 2 cut(s) 492, 850
Fnu4HI GCNGC 7 cut(s) 198, 210, 692, 1069, 1209, 1212, 1215
FokI GGATG 2 cut(s) 533, 1163
FriOI GRGCYC 1 cut(s) 169
Fsp4HI GCNGC 7 cut(s) 198, 210, 692, 1069, 1209, 1212, 1215
FspBI CTAG 1 cut(s) 170
FspI TGCGCA 1 cut(s) 12
GlaI GCGC 2 cut(s) 12, 335
GluI GCNGC 7 cut(s) 198, 210, 692, 1069, 1209, 1212, 1215
HapII CCGG 3 cut(s) 137, 267, 573
HgaI GACGC 2 cut(s) 325, 450
HhaI GCGC 2 cut(s) 13, 336
Hin1II CATG 6 cut(s) 567, 605, 623, 943, 1068, 1222
Hin6I GCGC 2 cut(s) 11, 334
HinP1I GCGC 2 cut(s) 11, 334
HincII GTYRAC 2 cut(s) 277, 591
HindII GTYRAC 2 cut(s) 277, 591
HindIII AAGCTT 2 cut(s) 944, 1171
HinfI GANTC 5 cut(s) 350, 515, 706, 1006, 1133
HpaII CCGG 3 cut(s) 137, 267, 573
Hpy166II GTNNAC 5 cut(s) 277, 526, 591, 617, 653
Hpy188III TCNNGA 4 cut(s) 235, 573, 629, 827
Hpy8I GTNNAC 5 cut(s) 277, 526, 591, 617, 653
Hpy99I CGWCG 4 cut(s) 188, 334, 341, 467
HpyAV CCTTC 8 cut(s) 103, 161, 335, 431, 440, 859, 1141, 1260
HpyCH4III ACNGT 3 cut(s) 274, 430, 1240
HpyCH4IV ACGT 2 cut(s) 294, 419
HpyCH4V TGCA 6 cut(s) 4, 501, 580, 617, 1071, 1208
HpyF10VI GCNNNNNNNGC 9 cut(s) 10, 206, 342, 389, 801, 844, 853, 1214, 1241
HpyF3I CTNAG 6 cut(s) 141, 348, 366, 453, 687, 1156
HpySE526I ACGT 2 cut(s) 294, 419
Hsp92II CATG 6 cut(s) 567, 605, 623, 943, 1068, 1222
HspAI GCGC 2 cut(s) 11, 334
Kpn2I TCCGGA 1 cut(s) 572
KpnI GGTACC 1 cut(s) 273
Ksp22I TGATCA 2 cut(s) 492, 850
Kzo9I GATC 5 cut(s) 239, 355, 492, 850, 873
LmnI GCTCC 6 cut(s) 48, 95, 366, 602, 1087, 1226
Lsp1109I GCAGC 5 cut(s) 678, 1055, 1198, 1201, 1220
LweI GCATC 2 cut(s) 629, 1141
MaeI CTAG 1 cut(s) 170
MaeII ACGT 2 cut(s) 294, 419
MaeIII GTNAC 3 cut(s) 202, 718, 820
MalI GATC 5 cut(s) 241, 357, 494, 852, 875
MbiI CCGCTC 2 cut(s) 361, 383
MboI GATC 5 cut(s) 239, 355, 492, 850, 873
MboII GAAGA 4 cut(s) 227, 289, 364, 903
MhlI GDGCHC 2 cut(s) 169, 619
MluCI AATT 2 cut(s) 432, 842
MlyI GAGTC 3 cut(s) 359, 524, 1142
MmeI TCCRAC 5 cut(s) 95, 207, 486, 498, 1204
MroI TCCGGA 1 cut(s) 572
MseI TTAA 1 cut(s) 414
MslI CAYNNNNRTG 2 cut(s) 750, 810
MspA1I CMGCKG 1 cut(s) 1211
MspI CCGG 3 cut(s) 137, 267, 573
MspR9I CCNGG 5 cut(s) 137, 325, 780, 977, 1144
MvaI CCWGG 4 cut(s) 325, 780, 977, 1144
MvnI CGCG 3 cut(s) 334, 336, 444
MwoI GCNNNNNNNGC 9 cut(s) 10, 206, 342, 389, 801, 844, 853, 1214, 1241
NciI CCSGG 1 cut(s) 137
NcoI CCATGG 2 cut(s) 563, 1218
NdeII GATC 5 cut(s) 239, 355, 492, 850, 873
NlaIII CATG 6 cut(s) 567, 605, 623, 943, 1068, 1222
NlaIV GGNNCC 3 cut(s) 271, 598, 896
NmuCI GTSAC 2 cut(s) 202, 820
NsbI TGCGCA 1 cut(s) 12
NspI RCATGY 1 cut(s) 1068
PceI AGGCCT 1 cut(s) 323
PcsI WCGNNNNNNNCGW 2 cut(s) 291, 408
PfeI GAWTC 2 cut(s) 706, 1006
PfoI TCCNGGA 1 cut(s) 975
PkrI GCNGC 7 cut(s) 199, 211, 693, 1070, 1210, 1213, 1216
PleI GAGTC 3 cut(s) 358, 523, 1141
PpsI GAGTC 3 cut(s) 358, 523, 1141
Psp6I CCWGG 4 cut(s) 323, 778, 975, 1142
PspGI CCWGG 4 cut(s) 323, 778, 975, 1142
PspN4I GGNNCC 3 cut(s) 271, 598, 896
PspPI GGNCC 2 cut(s) 653, 895
PstNI CAGNNNCTG 1 cut(s) 1214
PvuII CAGCTG 1 cut(s) 1211
RsaI GTAC 1 cut(s) 271
RsaNI GTAC 1 cut(s) 270
RseI CAYNNNNRTG 2 cut(s) 750, 810
SaqAI TTAA 1 cut(s) 414
SatI GCNGC 7 cut(s) 198, 210, 692, 1069, 1209, 1212, 1215
Sau3AI GATC 5 cut(s) 239, 355, 492, 850, 873
Sau96I GGNCC 2 cut(s) 653, 895
SchI GAGTC 3 cut(s) 359, 524, 1142
ScrFI CCNGG 5 cut(s) 137, 325, 780, 977, 1144
SduI GDGCHC 2 cut(s) 169, 619
SfaNI GCATC 2 cut(s) 629, 1141
SfcI CTRYAG 2 cut(s) 772, 1252
SinI GGWCC 1 cut(s) 653
SmiMI CAYNNNNRTG 2 cut(s) 750, 810
Sse9I AATT 2 cut(s) 432, 842
SseBI AGGCCT 1 cut(s) 323
SsiI CCGC 7 cut(s) 34, 198, 210, 289, 359, 381, 642
SspMI CTAG 1 cut(s) 170
StuI AGGCCT 1 cut(s) 323
StyD4I CCNGG 5 cut(s) 135, 323, 778, 975, 1142
StyI CCWWGG 3 cut(s) 563, 1039, 1218
TaaI ACNGT 3 cut(s) 274, 430, 1240
TaiI ACGT 2 cut(s) 297, 422
TaqI TCGA 3 cut(s) 339, 628, 879
TasI AATT 2 cut(s) 432, 842
TauI GCSGC 2 cut(s) 200, 212
TfiI GAWTC 2 cut(s) 706, 1006
Tru1I TTAA 1 cut(s) 414
Tru9I TTAA 1 cut(s) 414
TscAI CASTG 2 cut(s) 1236, 1245
TseFI GTSAC 2 cut(s) 202, 820
TseI GCWGC 5 cut(s) 691, 1068, 1208, 1211, 1214
Tsp45I GTSAC 2 cut(s) 202, 820
TspDTI ATGAA 3 cut(s) 933, 947, 956
TspRI CASTG 2 cut(s) 1236, 1245
VneI GTGCAC 1 cut(s) 615
VpaK11BI GGWCC 1 cut(s) 653
XceI RCATGY 1 cut(s) 1068
XcmI CCANNNNNNNNNTGG 1 cut(s) 752
XspI CTAG 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.