AT3G60660

Spindle and kinetochore-associated protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001324
Physical Location & Seq
Forward (+)
22421344 .. 22423477
2134 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G60660.1

Sequence Viewer

Length: 819 bp
ATGGAGGGAAATCAAGCTGGATCTTCTCTAGATTCGTTGATTGCTTCTTTCAATAAACGAATCGGCGAATTGCAGGAACTCGTCATCGCCAGAAACATGTATCCTGCAAGTACCATCCCGGATTTATCGGCGATTGACACAGCGTTGAGCTCAATGGAGCTTCAGGTACAATCGATCAAGGACCGATTACGTGAAGAAACCGAAGCTATTCCAAAAGCTAAGAAACTTATAGAGGCATCACTGAAACAACAAGGGAAGTTGCAGAAGATGTCTATTTATGCACCTTCCCATTTTCCTGACAAGGCTACTATGTTGAATTCAGATTTAAATAGATGTCTGCTCCAAGAGAATGCCAAGCAGTACGAGCAACATTCTACTCTCAGCTCTTTGAAATTTGATGAGGAAGCAGCTGTTTTACCCAAGGAGAAGAAGGGTCGCGGGTCTCCTCCCCTTTGGTACATAACTGTTGAGGAACTCAATTCACTCTCATCATACATGAGAGGAAGGCTCACCCTTGAGAAAGTGAATGCTGCTATCAATGACATGGCCTCATATGCTGAAGCAAATGCTCATCTTATTGCAGCCTCAAAACAGAAGCTAGCGGAAAATCTCTGGGAGAAAGCACTTAAACTACGAGATATCGTAACAGAGCAAGCAGTGAAGGGCAAACATTTCTTTCTTGAAACTGACATGAAGGGACCAAGTTTGAAACTAGACAACACAGGAAAAGCAATACTTACTGTTCTTCGCCACCTTGGTCGTATTAGTGAGACTCGCATTGGACAAAACCGCGTCATCATTCTTATGAAGCCTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

272

Amino Acids

30.4

Weight (kDa)

9.1

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 8 - 262 4.8e-86 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 438, 792
AciI CCGC 3 cut(s) 438, 602, 790
AclWI GGATC 1 cut(s) 28
AcsI RAATTY 2 cut(s) 316, 392
AcuI CTGAAG 2 cut(s) 146, 579
AfaI GTAC 4 cut(s) 112, 168, 362, 458
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 5 cut(s) 52, 316, 391, 683, 709
AluBI AGCT 8 cut(s) 17, 150, 160, 206, 218, 384, 410, 598
AluI AGCT 8 cut(s) 17, 150, 160, 206, 218, 384, 410, 598
Alw21I GWGCWC 1 cut(s) 152
Alw26I GTCTC 2 cut(s) 447, 764
AlwI GGATC 1 cut(s) 28
AoxI GGCC 1 cut(s) 546
ApeKI GCWGC 3 cut(s) 407, 530, 581
ApoI RAATTY 2 cut(s) 316, 392
Asp700I GAANNNNTTC 1 cut(s) 207
AspS9I GGNCC 2 cut(s) 181, 698
AsuC2I CCSGG 1 cut(s) 119
AsuHPI GGTGA 1 cut(s) 502
AsuNHI GCTAGC 1 cut(s) 598
AvaII GGWCC 2 cut(s) 181, 698
BanII GRGCYC 1 cut(s) 152
Bbv12I GWGCWC 1 cut(s) 152
BbvI GCAGC 3 cut(s) 419, 517, 593
BccI CCATC 1 cut(s) 122
BciVI GTATCC 1 cut(s) 111
BcnI CCSGG 1 cut(s) 119
BcoDI GTCTC 2 cut(s) 447, 764
BfaI CTAG 4 cut(s) 29, 599, 713, 817
BfuI GTATCC 1 cut(s) 111
BisI GCNGC 3 cut(s) 408, 531, 582
BlsI GCNGC 3 cut(s) 409, 532, 583
Bme1390I CCNGG 1 cut(s) 119
Bme18I GGWCC 2 cut(s) 181, 698
BmgT120I GGNCC 2 cut(s) 181, 698
BmiI GGNNCC 1 cut(s) 699
BmrFI CCNGG 1 cut(s) 119
BmsI GCATC 1 cut(s) 245
BmtI GCTAGC 1 cut(s) 602
BplI GAGNNNNNCTC 2 cut(s) 492, 524
BpuEI CTTGAG 1 cut(s) 536
BpuMI CCSGG 1 cut(s) 119
Bsa29I ATCGAT 1 cut(s) 173
BsaAI YACGTR 1 cut(s) 191
BsaI GGTCTC 1 cut(s) 447
BsaJI CCNNGG 2 cut(s) 420, 754
BseCI ATCGAT 1 cut(s) 173
BseDI CCNNGG 2 cut(s) 420, 754
BseGI GGATG 1 cut(s) 114
BseMII CTCAG 1 cut(s) 394
BseRI GAGGAG 1 cut(s) 435
BseXI GCAGC 3 cut(s) 419, 517, 593
Bsh1236I CGCG 2 cut(s) 438, 792
BshFI GGCC 1 cut(s) 548
BshVI ATCGAT 1 cut(s) 173
BsiHKAI GWGCWC 1 cut(s) 152
BsiSI CCGG 1 cut(s) 119
BslFI GGGAC 1 cut(s) 711
BsmAI GTCTC 2 cut(s) 447, 764
BsmFI GGGAC 1 cut(s) 711
BsmI GAATGC 2 cut(s) 355, 532
BsnI GGCC 1 cut(s) 548
Bso31I GGTCTC 1 cut(s) 447
Bsp1286I GDGCHC 1 cut(s) 152
Bsp143I GATC 2 cut(s) 20, 174
BspACI CCGC 3 cut(s) 438, 602, 790
BspANI GGCC 1 cut(s) 548
BspCNI CTCAG 1 cut(s) 393
BspDI ATCGAT 1 cut(s) 173
BspFNI CGCG 2 cut(s) 438, 792
BspLI GGNNCC 1 cut(s) 699
BspOI GCTAGC 1 cut(s) 602
BspPI GGATC 1 cut(s) 28
BspTNI GGTCTC 1 cut(s) 447
BssECI CCNNGG 2 cut(s) 420, 754
BssMI GATC 2 cut(s) 20, 174
BssT1I CCWWGG 2 cut(s) 420, 754
Bst4CI ACNGT 2 cut(s) 466, 742
BstBAI YACGTR 1 cut(s) 191
BstC8I GCNNGC 2 cut(s) 600, 654
BstDEI CTNAG 2 cut(s) 219, 380
BstF5I GGATG 1 cut(s) 114
BstFNI CGCG 2 cut(s) 438, 792
BstKTI GATC 2 cut(s) 23, 177
BstMAI GTCTC 2 cut(s) 447, 764
BstMBI GATC 2 cut(s) 20, 174
BstMWI GCNNNNNNNGC 2 cut(s) 364, 554
BstNSI RCATGY 1 cut(s) 100
BstSCI CCNGG 1 cut(s) 117
BstUI CGCG 2 cut(s) 438, 792
BstV1I GCAGC 3 cut(s) 419, 517, 593
BstX2I RGATCY 1 cut(s) 20
BstYI RGATCY 1 cut(s) 20
Bsu15I ATCGAT 1 cut(s) 173
BsuI GTATCC 1 cut(s) 111
BsuRI GGCC 1 cut(s) 548
BsuTUI ATCGAT 1 cut(s) 173
BtgZI GCGATG 1 cut(s) 70
BtsCI GGATG 1 cut(s) 114
BtsI GCAGTG 1 cut(s) 663
BtsIMutI CAGTG 2 cut(s) 239, 663
Cac8I GCNNGC 2 cut(s) 600, 654
Cfr13I GGNCC 2 cut(s) 181, 698
ClaI ATCGAT 1 cut(s) 173
CseI GACGC 1 cut(s) 781
Csp6I GTAC 4 cut(s) 111, 167, 361, 457
CviAII CATG 4 cut(s) 97, 496, 544, 691
CviQI GTAC 4 cut(s) 111, 167, 361, 457
DdeI CTNAG 2 cut(s) 219, 380
DpnI GATC 2 cut(s) 22, 176
DpnII GATC 2 cut(s) 20, 174
DraI TTTAAA 1 cut(s) 327
Ecl136II GAGCTC 1 cut(s) 150
Eco130I CCWWGG 2 cut(s) 420, 754
Eco24I GRGCYC 1 cut(s) 152
Eco31I GGTCTC 1 cut(s) 447
Eco32I GATATC 1 cut(s) 640
Eco47I GGWCC 2 cut(s) 181, 698
Eco53kI GAGCTC 1 cut(s) 150
Eco57I CTGAAG 2 cut(s) 146, 579
EcoICRI GAGCTC 1 cut(s) 150
EcoRI GAATTC 1 cut(s) 316
EcoRV GATATC 1 cut(s) 640
EcoT14I CCWWGG 2 cut(s) 420, 754
EcoT38I GRGCYC 1 cut(s) 152
ErhI CCWWGG 2 cut(s) 420, 754
FaeI CATG 4 cut(s) 100, 499, 547, 694
FalI AAGNNNNNCTT 2 cut(s) 720, 752
FaqI GGGAC 1 cut(s) 711
FatI CATG 4 cut(s) 96, 495, 543, 690
FauI CCCGC 1 cut(s) 431
FauNDI CATATG 1 cut(s) 553
Fnu4HI GCNGC 3 cut(s) 408, 531, 582
FokI GGATG 1 cut(s) 101
FriOI GRGCYC 1 cut(s) 152
Fsp4HI GCNGC 3 cut(s) 408, 531, 582
FspBI CTAG 4 cut(s) 29, 599, 713, 817
GluI GCNGC 3 cut(s) 408, 531, 582
HaeIII GGCC 1 cut(s) 548
HapII CCGG 1 cut(s) 119
HgaI GACGC 1 cut(s) 781
Hin1II CATG 4 cut(s) 100, 499, 547, 694
HinfI GANTC 3 cut(s) 32, 60, 772
HpaII CCGG 1 cut(s) 119
HphI GGTGA 1 cut(s) 502
Hpy188I TCNGA 1 cut(s) 322
Hpy188III TCNNGA 3 cut(s) 29, 296, 680
HpyAV CCTTC 5 cut(s) 294, 424, 498, 655, 688
HpyCH4III ACNGT 2 cut(s) 466, 742
HpyCH4IV ACGT 1 cut(s) 190
HpyCH4V TGCA 5 cut(s) 73, 107, 262, 281, 581
HpyF10VI GCNNNNNNNGC 2 cut(s) 364, 554
HpyF3I CTNAG 2 cut(s) 219, 380
HpySE526I ACGT 1 cut(s) 190
Hsp92II CATG 4 cut(s) 100, 499, 547, 694
Kzo9I GATC 2 cut(s) 20, 174
LmnI GCTCC 2 cut(s) 157, 345
LpnPI CCDG 9 cut(s) 3, 59, 103, 117, 132, 149, 309, 598, 708
Lsp1109I GCAGC 3 cut(s) 419, 517, 593
LweI GCATC 1 cut(s) 245
MaeI CTAG 4 cut(s) 29, 599, 713, 817
MaeII ACGT 1 cut(s) 190
MaeIII GTNAC 1 cut(s) 643
MalI GATC 2 cut(s) 22, 176
MboI GATC 2 cut(s) 20, 174
MboII GAAGA 5 cut(s) 15, 206, 277, 439, 737
MflI RGATCY 1 cut(s) 20
MhlI GDGCHC 1 cut(s) 152
MluCI AATT 4 cut(s) 68, 316, 392, 478
MlyI GAGTC 1 cut(s) 766
MnlI CCTC 7 cut(s) 226, 394, 456, 463, 494, 559, 595
MroXI GAANNNNTTC 1 cut(s) 207
MseI TTAA 2 cut(s) 326, 627
MslI CAYNNNNRTG 1 cut(s) 803
MspA1I CMGCKG 1 cut(s) 410
MspI CCGG 1 cut(s) 119
MspR9I CCNGG 1 cut(s) 119
Mva1269I GAATGC 2 cut(s) 355, 532
MvnI CGCG 2 cut(s) 438, 792
MwoI GCNNNNNNNGC 2 cut(s) 364, 554
NciI CCSGG 1 cut(s) 119
NdeI CATATG 1 cut(s) 553
NdeII GATC 2 cut(s) 20, 174
NheI GCTAGC 1 cut(s) 598
NlaIII CATG 4 cut(s) 100, 499, 547, 694
NlaIV GGNNCC 1 cut(s) 699
NspI RCATGY 1 cut(s) 100
PciI ACATGT 1 cut(s) 96
PctI GAATGC 2 cut(s) 355, 532
PdmI GAANNNNTTC 1 cut(s) 207
PfeI GAWTC 2 cut(s) 32, 60
PfoI TCCNGGA 1 cut(s) 117
PkrI GCNGC 3 cut(s) 409, 532, 583
PleI GAGTC 1 cut(s) 766
PpsI GAGTC 1 cut(s) 766
Ppu21I YACGTR 1 cut(s) 191
PscI ACATGT 1 cut(s) 96
Psp124BI GAGCTC 1 cut(s) 152
PspN4I GGNNCC 1 cut(s) 699
PspPI GGNCC 2 cut(s) 181, 698
PsuI RGATCY 1 cut(s) 20
PvuII CAGCTG 1 cut(s) 410
RsaI GTAC 4 cut(s) 112, 168, 362, 458
RsaNI GTAC 4 cut(s) 111, 167, 361, 457
RseI CAYNNNNRTG 1 cut(s) 803
SacI GAGCTC 1 cut(s) 152
SaqAI TTAA 2 cut(s) 326, 627
SatI GCNGC 3 cut(s) 408, 531, 582
Sau3AI GATC 2 cut(s) 20, 174
Sau96I GGNCC 2 cut(s) 181, 698
SchI GAGTC 1 cut(s) 766
ScrFI CCNGG 1 cut(s) 119
SduI GDGCHC 1 cut(s) 152
SfaNI GCATC 1 cut(s) 245
SinI GGWCC 2 cut(s) 181, 698
SmiI ATTTAAAT 1 cut(s) 327
SmiMI CAYNNNNRTG 1 cut(s) 803
SmlI CTYRAG 1 cut(s) 515
SmoI CTYRAG 1 cut(s) 515
Sse9I AATT 4 cut(s) 68, 316, 392, 478
SsiI CCGC 3 cut(s) 438, 602, 790
SspMI CTAG 4 cut(s) 29, 599, 713, 817
SstI GAGCTC 1 cut(s) 152
StyD4I CCNGG 1 cut(s) 117
StyI CCWWGG 2 cut(s) 420, 754
SwaI ATTTAAAT 1 cut(s) 327
TaaI ACNGT 2 cut(s) 466, 742
TaiI ACGT 1 cut(s) 193
TaqI TCGA 1 cut(s) 173
TaqII GACCGA 1 cut(s) 198
TasI AATT 4 cut(s) 68, 316, 392, 478
TfiI GAWTC 2 cut(s) 32, 60
Tru1I TTAA 2 cut(s) 326, 627
Tru9I TTAA 2 cut(s) 326, 627
TscAI CASTG 2 cut(s) 246, 663
TseI GCWGC 3 cut(s) 407, 530, 581
TspDTI ATGAA 1 cut(s) 707
TspRI CASTG 2 cut(s) 246, 663
VpaK11BI GGWCC 2 cut(s) 181, 698
XapI RAATTY 2 cut(s) 316, 392
XbaI TCTAGA 1 cut(s) 28
XceI RCATGY 1 cut(s) 100
XmnI GAANNNNTTC 1 cut(s) 207
XspI CTAG 4 cut(s) 29, 599, 713, 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.