Rh1AG218700

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0001324
Physical Location & Seq
Forward (+)
41578090 .. 41579316
1227 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG218700.1

Sequence Viewer

Length: 357 bp
ATGGAGGCGGAGAAAGCAGGGGCATCGCTGGACTCGCTGATCGCCGGGTTCAACAGTCGGATCTCGGAGCTCCAAGAGCTCGTTATTGCGCGAAACATGTACCCGGCCAGCACAGTCTCCGATTTGTCGGCCGTCGATGCCGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGCCATCAAGGACCGGGTTCGCGACGAAACCCTTGCCATCCCCAAAGCAAAACAAAACACTGGTTCTGGGACCTTCAAGCTTGAGGAGGATCCTGCACCACCACCACCACCCAAGGAGAAAAAGGGTCGTTCCATGCCACCATTGTGGTTTGTAAGTGCTGAAGAGCTGGATTCCTTGTCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

118

Amino Acids

12.71

Weight (kDa)

4.87

Isoelectric Point (pI)

52.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 9 - 75 1.2e-15 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 91, 192
AciI CCGC 2 cut(s) 8, 141
AclWI GGATC 3 cut(s) 68, 254, 267
AcoI YGGCCR 2 cut(s) 105, 129
AcuI CTGAAG 2 cut(s) 146, 351
AfaI GTAC 1 cut(s) 101
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 2 cut(s) 52, 247
AjnI CCWGG 1 cut(s) 168
AleI CACNNNNGTG 1 cut(s) 313
AluBI AGCT 5 cut(s) 70, 79, 160, 250, 337
AluI AGCT 5 cut(s) 70, 79, 160, 250, 337
Alw21I GWGCWC 2 cut(s) 72, 81
Alw26I GTCTC 1 cut(s) 121
AlwI GGATC 3 cut(s) 68, 254, 267
AoxI GGCC 4 cut(s) 105, 129, 150, 171
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 3 cut(s) 166, 181, 240
AsuC2I CCSGG 3 cut(s) 46, 104, 185
AvaII GGWCC 3 cut(s) 166, 181, 240
BamHI GGATCC 1 cut(s) 259
BanII GRGCYC 2 cut(s) 72, 81
Bbv12I GWGCWC 2 cut(s) 72, 81
BccI CCATC 2 cut(s) 182, 215
BceAI ACGGC 2 cut(s) 116, 128
BcgI CGANNNNNNTGC 4 cut(s) 6, 40, 185, 219
BciT130I CCWGG 1 cut(s) 170
BcnI CCSGG 3 cut(s) 46, 104, 185
BcoDI GTCTC 1 cut(s) 121
BglI GCCNNNNNGGC 1 cut(s) 149
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
Bme1390I CCNGG 4 cut(s) 46, 104, 170, 185
Bme18I GGWCC 3 cut(s) 166, 181, 240
BmgT120I GGNCC 3 cut(s) 166, 181, 240
BmiI GGNNCC 2 cut(s) 241, 261
BmrFI CCNGG 4 cut(s) 46, 104, 170, 185
BmsI GCATC 2 cut(s) 32, 127
BpuEI CTTGAG 1 cut(s) 272
BpuMI CCSGG 3 cut(s) 46, 104, 185
BsaJI CCNNGG 2 cut(s) 153, 282
Bse1I ACTGG 1 cut(s) 235
BseBI CCWGG 1 cut(s) 170
BseDI CCNNGG 2 cut(s) 153, 282
BseGI GGATG 1 cut(s) 207
BseNI ACTGG 1 cut(s) 235
BseRI GAGGAG 1 cut(s) 269
BseX3I CGGCCG 1 cut(s) 129
BsgI GTGCAG 1 cut(s) 249
Bsh1236I CGCG 2 cut(s) 91, 192
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 4 cut(s) 107, 131, 152, 173
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 2 cut(s) 72, 81
BsiSI CCGG 3 cut(s) 45, 104, 184
BslFI GGGAC 1 cut(s) 253
BsmAI GTCTC 1 cut(s) 121
BsmFI GGGAC 1 cut(s) 253
BsnI GGCC 4 cut(s) 107, 131, 152, 173
Bsp1286I GDGCHC 2 cut(s) 72, 81
Bsp143I GATC 3 cut(s) 39, 60, 259
Bsp19I CCATGG 1 cut(s) 153
Bsp68I TCGCGA 1 cut(s) 192
BspACI CCGC 2 cut(s) 8, 141
BspANI GGCC 4 cut(s) 107, 131, 152, 173
BspFNI CGCG 2 cut(s) 91, 192
BspLI GGNNCC 2 cut(s) 241, 261
BspPI GGATC 3 cut(s) 68, 254, 267
BspQI GCTCTTC 1 cut(s) 327
BsrI ACTGG 1 cut(s) 235
BssECI CCNNGG 2 cut(s) 153, 282
BssMI GATC 3 cut(s) 39, 60, 259
BssT1I CCWWGG 2 cut(s) 153, 282
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 2 cut(s) 56, 115
Bst6I CTCTTC 1 cut(s) 327
BstC8I GCNNGC 1 cut(s) 109
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 1 cut(s) 207
BstFNI CGCG 2 cut(s) 91, 192
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 3 cut(s) 42, 63, 262
BstMAI GTCTC 1 cut(s) 121
BstMBI GATC 3 cut(s) 39, 60, 259
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 5 cut(s) 14, 34, 76, 137, 149
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 100
BstSCI CCNGG 4 cut(s) 44, 102, 168, 183
BstUI CGCG 2 cut(s) 91, 192
BstX2I RGATCY 2 cut(s) 60, 259
BstXI CCANNNNNNTGG 1 cut(s) 315
BstYI RGATCY 2 cut(s) 60, 259
BstZI CGGCCG 1 cut(s) 129
BsuRI GGCC 4 cut(s) 107, 131, 152, 173
BtgI CCRYGG 1 cut(s) 153
BtgZI GCGATG 1 cut(s) 9
BtsCI GGATG 1 cut(s) 207
BtsIMutI CAGTG 1 cut(s) 228
BtuMI TCGCGA 1 cut(s) 192
Cac8I GCNNGC 1 cut(s) 109
CfoI GCGC 1 cut(s) 91
Cfr13I GGNCC 3 cut(s) 166, 181, 240
Csp6I GTAC 1 cut(s) 100
CviAII CATG 3 cut(s) 97, 154, 304
CviJI RGCY 9 cut(s) 70, 79, 107, 131, 152, 160, 173, 250, 337
CviKI_1 RGCY 9 cut(s) 70, 79, 107, 131, 152, 160, 173, 250, 337
CviQI GTAC 1 cut(s) 100
DpnI GATC 3 cut(s) 41, 62, 261
DpnII GATC 3 cut(s) 39, 60, 259
EaeI YGGCCR 2 cut(s) 105, 129
EagI CGGCCG 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 2 cut(s) 70, 79
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 2 cut(s) 153, 282
Eco24I GRGCYC 2 cut(s) 72, 81
Eco47I GGWCC 3 cut(s) 166, 181, 240
Eco52I CGGCCG 1 cut(s) 129
Eco53kI GAGCTC 2 cut(s) 70, 79
Eco57I CTGAAG 2 cut(s) 146, 351
EcoICRI GAGCTC 2 cut(s) 70, 79
EcoO109I RGGNCCY 1 cut(s) 240
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 2 cut(s) 153, 282
EcoT38I GRGCYC 2 cut(s) 72, 81
ErhI CCWWGG 2 cut(s) 153, 282
FaeI CATG 3 cut(s) 100, 157, 307
FaiI YATR 3 cut(s) 98, 155, 305
FaqI GGGAC 1 cut(s) 253
FatI CATG 3 cut(s) 96, 153, 303
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 1 cut(s) 194
FriOI GRGCYC 2 cut(s) 72, 81
Fsp4HI GCNGC 1 cut(s) 141
GlaI GCGC 1 cut(s) 90
GluI GCNGC 1 cut(s) 141
HaeIII GGCC 4 cut(s) 107, 131, 152, 173
HapII CCGG 3 cut(s) 45, 104, 184
HhaI GCGC 1 cut(s) 91
Hin1II CATG 3 cut(s) 100, 157, 307
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HindIII AAGCTT 1 cut(s) 248
HinfI GANTC 2 cut(s) 32, 341
HpaII CCGG 3 cut(s) 45, 104, 184
Hpy188I TCNGA 3 cut(s) 60, 67, 121
Hpy188III TCNNGA 2 cut(s) 191, 354
Hpy99I CGWCG 2 cut(s) 137, 197
HpyAV CCTTC 2 cut(s) 142, 253
HpyCH4III ACNGT 2 cut(s) 56, 115
HpyCH4V TGCA 1 cut(s) 266
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 34, 76, 137, 149
Hsp92II CATG 3 cut(s) 100, 157, 307
HspAI GCGC 1 cut(s) 89
Kzo9I GATC 3 cut(s) 39, 60, 259
LguI GCTCTTC 1 cut(s) 327
LmnI GCTCC 3 cut(s) 67, 75, 157
LweI GCATC 2 cut(s) 32, 127
MalI GATC 3 cut(s) 41, 62, 261
MboI GATC 3 cut(s) 39, 60, 259
MboII GAAGA 1 cut(s) 344
MflI RGATCY 2 cut(s) 60, 259
MhlI GDGCHC 2 cut(s) 72, 81
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 1 cut(s) 38
MnlI CCTC 2 cut(s) 247, 250
MslI CAYNNNNRTG 1 cut(s) 313
MspI CCGG 3 cut(s) 45, 104, 184
MspR9I CCNGG 4 cut(s) 46, 104, 170, 185
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 2 cut(s) 91, 192
MwoI GCNNNNNNNGC 5 cut(s) 14, 34, 76, 137, 149
NciI CCSGG 3 cut(s) 46, 104, 185
NcoI CCATGG 1 cut(s) 153
NdeII GATC 3 cut(s) 39, 60, 259
NlaIII CATG 3 cut(s) 100, 157, 307
NlaIV GGNNCC 2 cut(s) 241, 261
NruI TCGCGA 1 cut(s) 192
NspI RCATGY 1 cut(s) 100
OliI CACNNNNGTG 1 cut(s) 313
PciI ACATGT 1 cut(s) 96
PciSI GCTCTTC 1 cut(s) 327
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PfeI GAWTC 1 cut(s) 341
PkrI GCNGC 1 cut(s) 142
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PpuMI RGGWCCY 1 cut(s) 240
PscI ACATGT 1 cut(s) 96
Psp124BI GAGCTC 2 cut(s) 72, 81
Psp5II RGGWCCY 1 cut(s) 240
Psp6I CCWGG 1 cut(s) 168
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 2 cut(s) 241, 261
PspPI GGNCC 3 cut(s) 166, 181, 240
PspPPI RGGWCCY 1 cut(s) 240
PsuI RGATCY 2 cut(s) 60, 259
RruI TCGCGA 1 cut(s) 192
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
RseI CAYNNNNRTG 1 cut(s) 313
SacI GAGCTC 2 cut(s) 72, 81
SapI GCTCTTC 1 cut(s) 327
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 3 cut(s) 39, 60, 259
Sau96I GGNCC 3 cut(s) 166, 181, 240
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 4 cut(s) 46, 104, 170, 185
SduI GDGCHC 2 cut(s) 72, 81
SetI ASST 7 cut(s) 72, 81, 162, 168, 245, 252, 339
SfaNI GCATC 2 cut(s) 32, 127
SinI GGWCC 3 cut(s) 166, 181, 240
SmiMI CAYNNNNRTG 1 cut(s) 313
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
SsiI CCGC 2 cut(s) 8, 141
SstI GAGCTC 2 cut(s) 72, 81
StyD4I CCNGG 4 cut(s) 44, 102, 168, 183
StyI CCWWGG 2 cut(s) 153, 282
TaaI ACNGT 2 cut(s) 56, 115
TaqI TCGA 1 cut(s) 135
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 1 cut(s) 341
TscAI CASTG 1 cut(s) 235
TspRI CASTG 1 cut(s) 235
VpaK11BI GGWCC 3 cut(s) 166, 181, 240
XceI RCATGY 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.