Rorug01G0201500

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001324
Physical Location & Seq
Forward (+)
28082685 .. 28083532
848 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0201500.1

Sequence Viewer

Length: 357 bp
ATGCCTGCGATGGGATTTGTGCCCAATGTGGTGACTTTTACAACCATTTTGGGCGGTTATGTGTCGCGGCGCGATATGGTTGGTGCCAAGAGGGTGTTTGGTGAGATTTTGGACAGAGGGTGGTTTCCTGATGTGACTACTTATACTATATTGATGGATGGGTATGTGAAGCAGGGGAAGCTGGTTGAGGCTATTAAGGTAATAGATGAGCTGGAGGATAATGGGGTTGGCGCCAATGAGGTTACTTATGGGGTTATGATTGAAGCTTATTGTAAGGAAAAGAAGTCGGGCGAAGCGGTTAATTTGCTTGATGATATGGTGGAGAAGAGACATCTTGTCATGATCAACAGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

118

Amino Acids

13.1

Weight (kDa)

4.95

Isoelectric Point (pI)

35.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 3 - 56 3.4e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 8 - 57 4.2e-14 PPR repeat family
PPR_3 PF13812 32 - 92 8.3e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 40 - 71 6.7e-08 PPR repeat
PPR_2 PF13041 45 - 92 5.2e-14 PPR repeat family
PPR PF01535 47 - 76 1.1e-07 PPR repeat
PPR_1 PF12854 75 - 107 2.6e-07 PPR repeat
PPR_2 PF13041 79 - 110 3e-07 PPR repeat family
PPR PF01535 81 - 110 2.3e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 83, 230
AccII CGCG 2 cut(s) 67, 72
AciI CCGC 3 cut(s) 54, 67, 296
AcyI GRCGYC 1 cut(s) 231
AfiI CCNNNNNNNGG 1 cut(s) 11
AgsI TTSAA 1 cut(s) 263
AhdI GACNNNNNGTC 1 cut(s) 335
AluBI AGCT 4 cut(s) 181, 211, 266, 351
AluI AGCT 4 cut(s) 181, 211, 266, 351
Alw26I GTCTC 1 cut(s) 322
AspLEI GCGC 2 cut(s) 72, 233
AsuHPI GGTGA 2 cut(s) 43, 113
BaeGI GKGCMC 1 cut(s) 24
BanI GGYRCC 2 cut(s) 83, 230
BccI CCATC 3 cut(s) 4, 148, 152
BclI TGATCA 1 cut(s) 342
BcoDI GTCTC 1 cut(s) 322
BfoI RGCGCY 1 cut(s) 234
BisI GCNGC 1 cut(s) 68
BlsI GCNGC 1 cut(s) 69
BmeRI GACNNNNNGTC 1 cut(s) 335
BmiI GGNNCC 2 cut(s) 85, 232
BpmI CTGGAG 1 cut(s) 233
BsaHI GRCGYC 1 cut(s) 231
Bsc4I CCNNNNNNNGG 1 cut(s) 11
BseGI GGATG 1 cut(s) 163
BseLI CCNNNNNNNGG 1 cut(s) 11
BseSI GKGCMC 1 cut(s) 24
Bsh1236I CGCG 2 cut(s) 67, 72
BshNI GGYRCC 2 cut(s) 83, 230
BslI CCNNNNNNNGG 1 cut(s) 11
BsmAI GTCTC 1 cut(s) 322
Bsp1286I GDGCHC 1 cut(s) 24
Bsp143I GATC 1 cut(s) 342
BspACI CCGC 3 cut(s) 54, 67, 296
BspFNI CGCG 2 cut(s) 67, 72
BspHI TCATGA 1 cut(s) 339
BspLI GGNNCC 2 cut(s) 85, 232
BspT107I GGYRCC 2 cut(s) 83, 230
BssMI GATC 1 cut(s) 342
BssNI GRCGYC 1 cut(s) 231
Bst6I CTCTTC 1 cut(s) 320
BstACI GRCGYC 1 cut(s) 231
BstC8I GCNNGC 1 cut(s) 6
BstF5I GGATG 1 cut(s) 163
BstFNI CGCG 2 cut(s) 67, 72
BstH2I RGCGCY 1 cut(s) 234
BstHHI GCGC 2 cut(s) 72, 233
BstKTI GATC 1 cut(s) 345
BstMAI GTCTC 1 cut(s) 322
BstMBI GATC 1 cut(s) 342
BstMWI GCNNNNNNNGC 1 cut(s) 178
BstSLI GKGCMC 1 cut(s) 24
BstUI CGCG 2 cut(s) 67, 72
BtgZI GCGATG 1 cut(s) 23
BtsCI GGATG 1 cut(s) 163
Cac8I GCNNGC 1 cut(s) 6
CciI TCATGA 1 cut(s) 339
CfoI GCGC 2 cut(s) 72, 233
CviAII CATG 1 cut(s) 340
CviJI RGCY 5 cut(s) 181, 191, 211, 266, 351
CviKI_1 RGCY 5 cut(s) 181, 191, 211, 266, 351
DinI GGCGCC 1 cut(s) 232
DpnI GATC 1 cut(s) 344
DpnII GATC 1 cut(s) 342
DriI GACNNNNNGTC 1 cut(s) 335
Eam1104I CTCTTC 1 cut(s) 320
Eam1105I GACNNNNNGTC 1 cut(s) 335
EarI CTCTTC 1 cut(s) 320
EgeI GGCGCC 1 cut(s) 232
EheI GGCGCC 1 cut(s) 232
FaeI CATG 1 cut(s) 343
FaiI YATR 9 cut(s) 60, 77, 144, 149, 165, 249, 257, 317, 341
FatI CATG 1 cut(s) 339
FbaI TGATCA 1 cut(s) 342
Fnu4HI GCNGC 1 cut(s) 68
FokI GGATG 1 cut(s) 170
Fsp4HI GCNGC 1 cut(s) 68
GlaI GCGC 2 cut(s) 71, 232
GluI GCNGC 1 cut(s) 68
GsuI CTGGAG 1 cut(s) 233
HaeII RGCGCY 1 cut(s) 234
HhaI GCGC 2 cut(s) 72, 233
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 1 cut(s) 343
Hin6I GCGC 2 cut(s) 70, 231
HinP1I GCGC 2 cut(s) 70, 231
HindIII AAGCTT 1 cut(s) 264
HphI GGTGA 2 cut(s) 43, 113
Hpy188III TCNNGA 3 cut(s) 128, 340, 354
HpyF10VI GCNNNNNNNGC 1 cut(s) 178
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 1 cut(s) 343
HspAI GCGC 2 cut(s) 70, 231
KasI GGCGCC 1 cut(s) 230
Ksp22I TGATCA 1 cut(s) 342
Kzo9I GATC 1 cut(s) 342
LpnPI CCDG 5 cut(s) 18, 141, 158, 167, 197
MaeIII GTNAC 3 cut(s) 31, 133, 241
MalI GATC 1 cut(s) 344
MboI GATC 1 cut(s) 342
MboII GAAGA 1 cut(s) 337
MhlI GDGCHC 1 cut(s) 24
MluCI AATT 1 cut(s) 301
Mly113I GGCGCC 1 cut(s) 231
MnlI CCTC 5 cut(s) 84, 110, 181, 208, 232
MseI TTAA 2 cut(s) 195, 300
MvnI CGCG 2 cut(s) 67, 72
MwoI GCNNNNNNNGC 1 cut(s) 178
NarI GGCGCC 1 cut(s) 231
NdeII GATC 1 cut(s) 342
NlaIII CATG 1 cut(s) 343
NlaIV GGNNCC 2 cut(s) 85, 232
NmuCI GTSAC 2 cut(s) 31, 133
PagI TCATGA 1 cut(s) 339
PkrI GCNGC 1 cut(s) 69
PluTI GGCGCC 1 cut(s) 234
PspN4I GGNNCC 2 cut(s) 85, 232
SaqAI TTAA 2 cut(s) 195, 300
SatI GCNGC 1 cut(s) 68
Sau3AI GATC 1 cut(s) 342
SduI GDGCHC 1 cut(s) 24
SetI ASST 6 cut(s) 183, 201, 213, 243, 268, 353
SfoI GGCGCC 1 cut(s) 232
Sse9I AATT 1 cut(s) 301
SsiI CCGC 3 cut(s) 54, 67, 296
SspDI GGCGCC 1 cut(s) 230
TasI AATT 1 cut(s) 301
TauI GCSGC 1 cut(s) 70
Tru1I TTAA 2 cut(s) 195, 300
Tru9I TTAA 2 cut(s) 195, 300
TseFI GTSAC 2 cut(s) 31, 133
Tsp45I GTSAC 2 cut(s) 31, 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.