Rroxscaffold_4G00305970

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001324
Physical Location & Seq
Reverse (-)
26741037 .. 26742541
1505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00305970.1

Sequence Viewer

Length: 294 bp
ATGGAGGCGGAGAAAGCAGGGGCATCGCTGGACTCGCTGATCGCCGGGTTCAACAGTCGGATCTCGGAGCTCCAAGAGCTCGTTATTGCGCGAAACATGTATCCGGCCAGCACCGTCTCCGATTTGTCGGCCGTCGATGCCGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGCCATCAAGGACCGGGTTCGCGACGAAACCCTTGCCATCCCCAAAGCAAAAGAGAAAAAGGGTTGTTCCATGCCACCATTGTGGTTTGTAAGTGCTGAAGAGCTGGATTCCTTGTCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

97

Amino Acids

10.47

Weight (kDa)

4.76

Isoelectric Point (pI)

41.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 9 - 76 4.4e-16 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 91, 192
AciI CCGC 2 cut(s) 8, 141
AclWI GGATC 1 cut(s) 68
AcoI YGGCCR 2 cut(s) 105, 129
AcuI CTGAAG 2 cut(s) 146, 288
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 1 cut(s) 52
AjnI CCWGG 1 cut(s) 168
AleI CACNNNNGTG 1 cut(s) 250
AluBI AGCT 4 cut(s) 70, 79, 160, 274
AluI AGCT 4 cut(s) 70, 79, 160, 274
Alw21I GWGCWC 2 cut(s) 72, 81
Alw26I GTCTC 1 cut(s) 121
AlwI GGATC 1 cut(s) 68
AoxI GGCC 4 cut(s) 105, 129, 150, 171
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 2 cut(s) 166, 181
AsuC2I CCSGG 2 cut(s) 46, 185
AvaII GGWCC 2 cut(s) 166, 181
BanII GRGCYC 2 cut(s) 72, 81
Bbv12I GWGCWC 2 cut(s) 72, 81
BccI CCATC 2 cut(s) 182, 215
BceAI ACGGC 2 cut(s) 116, 128
BcgI CGANNNNNNTGC 4 cut(s) 6, 40, 185, 219
BciT130I CCWGG 1 cut(s) 170
BciVI GTATCC 1 cut(s) 111
BcnI CCSGG 2 cut(s) 46, 185
BcoDI GTCTC 1 cut(s) 121
BfuI GTATCC 1 cut(s) 111
BglI GCCNNNNNGGC 1 cut(s) 149
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
Bme1390I CCNGG 3 cut(s) 46, 170, 185
Bme18I GGWCC 2 cut(s) 166, 181
BmgT120I GGNCC 2 cut(s) 166, 181
BmrFI CCNGG 3 cut(s) 46, 170, 185
BmsI GCATC 2 cut(s) 32, 127
BpuMI CCSGG 2 cut(s) 46, 185
BsaJI CCNNGG 1 cut(s) 153
BseBI CCWGG 1 cut(s) 170
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 1 cut(s) 207
BseX3I CGGCCG 1 cut(s) 129
Bsh1236I CGCG 2 cut(s) 91, 192
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 4 cut(s) 107, 131, 152, 173
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 2 cut(s) 72, 81
BsiSI CCGG 3 cut(s) 45, 104, 184
BsmAI GTCTC 1 cut(s) 121
BsmBI CGTCTC 1 cut(s) 121
BsnI GGCC 4 cut(s) 107, 131, 152, 173
Bsp1286I GDGCHC 2 cut(s) 72, 81
Bsp143I GATC 2 cut(s) 39, 60
Bsp19I CCATGG 1 cut(s) 153
Bsp68I TCGCGA 1 cut(s) 192
BspACI CCGC 2 cut(s) 8, 141
BspANI GGCC 4 cut(s) 107, 131, 152, 173
BspFNI CGCG 2 cut(s) 91, 192
BspPI GGATC 1 cut(s) 68
BspQI GCTCTTC 1 cut(s) 264
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 2 cut(s) 39, 60
BssT1I CCWWGG 1 cut(s) 153
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 2 cut(s) 56, 115
Bst6I CTCTTC 1 cut(s) 264
BstC8I GCNNGC 1 cut(s) 109
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 1 cut(s) 207
BstFNI CGCG 2 cut(s) 91, 192
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 2 cut(s) 42, 63
BstMAI GTCTC 1 cut(s) 121
BstMBI GATC 2 cut(s) 39, 60
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 5 cut(s) 14, 34, 76, 137, 149
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 100
BstSCI CCNGG 3 cut(s) 44, 168, 183
BstUI CGCG 2 cut(s) 91, 192
BstX2I RGATCY 1 cut(s) 60
BstXI CCANNNNNNTGG 1 cut(s) 252
BstYI RGATCY 1 cut(s) 60
BstZI CGGCCG 1 cut(s) 129
BsuI GTATCC 1 cut(s) 111
BsuRI GGCC 4 cut(s) 107, 131, 152, 173
BtgI CCRYGG 1 cut(s) 153
BtgZI GCGATG 1 cut(s) 9
BtsCI GGATG 1 cut(s) 207
BtuMI TCGCGA 1 cut(s) 192
Cac8I GCNNGC 1 cut(s) 109
CfoI GCGC 1 cut(s) 91
Cfr13I GGNCC 2 cut(s) 166, 181
CviAII CATG 3 cut(s) 97, 154, 241
CviJI RGCY 8 cut(s) 70, 79, 107, 131, 152, 160, 173, 274
CviKI_1 RGCY 8 cut(s) 70, 79, 107, 131, 152, 160, 173, 274
DpnI GATC 2 cut(s) 41, 62
DpnII GATC 2 cut(s) 39, 60
EaeI YGGCCR 2 cut(s) 105, 129
EagI CGGCCG 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 2 cut(s) 70, 79
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 153
Eco24I GRGCYC 2 cut(s) 72, 81
Eco47I GGWCC 2 cut(s) 166, 181
Eco52I CGGCCG 1 cut(s) 129
Eco53kI GAGCTC 2 cut(s) 70, 79
Eco57I CTGAAG 2 cut(s) 146, 288
EcoICRI GAGCTC 2 cut(s) 70, 79
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 1 cut(s) 153
EcoT38I GRGCYC 2 cut(s) 72, 81
ErhI CCWWGG 1 cut(s) 153
Esp3I CGTCTC 1 cut(s) 121
FaeI CATG 3 cut(s) 100, 157, 244
FaiI YATR 3 cut(s) 98, 155, 242
FatI CATG 3 cut(s) 96, 153, 240
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 1 cut(s) 194
FriOI GRGCYC 2 cut(s) 72, 81
Fsp4HI GCNGC 1 cut(s) 141
GlaI GCGC 1 cut(s) 90
GluI GCNGC 1 cut(s) 141
HaeIII GGCC 4 cut(s) 107, 131, 152, 173
HapII CCGG 3 cut(s) 45, 104, 184
HhaI GCGC 1 cut(s) 91
Hin1II CATG 3 cut(s) 100, 157, 244
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HinfI GANTC 2 cut(s) 32, 278
HpaII CCGG 3 cut(s) 45, 104, 184
Hpy188I TCNGA 3 cut(s) 60, 67, 121
Hpy188III TCNNGA 2 cut(s) 191, 291
Hpy99I CGWCG 2 cut(s) 137, 197
HpyAV CCTTC 1 cut(s) 142
HpyCH4III ACNGT 2 cut(s) 56, 115
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 34, 76, 137, 149
Hsp92II CATG 3 cut(s) 100, 157, 244
HspAI GCGC 1 cut(s) 89
Kzo9I GATC 2 cut(s) 39, 60
LguI GCTCTTC 1 cut(s) 264
LmnI GCTCC 3 cut(s) 67, 75, 157
LweI GCATC 2 cut(s) 32, 127
MalI GATC 2 cut(s) 41, 62
MboI GATC 2 cut(s) 39, 60
MboII GAAGA 1 cut(s) 281
MflI RGATCY 1 cut(s) 60
MhlI GDGCHC 2 cut(s) 72, 81
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 1 cut(s) 38
MslI CAYNNNNRTG 1 cut(s) 250
MspI CCGG 3 cut(s) 45, 104, 184
MspR9I CCNGG 3 cut(s) 46, 170, 185
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 2 cut(s) 91, 192
MwoI GCNNNNNNNGC 5 cut(s) 14, 34, 76, 137, 149
NciI CCSGG 2 cut(s) 46, 185
NcoI CCATGG 1 cut(s) 153
NdeII GATC 2 cut(s) 39, 60
NlaIII CATG 3 cut(s) 100, 157, 244
NruI TCGCGA 1 cut(s) 192
NspI RCATGY 1 cut(s) 100
OliI CACNNNNGTG 1 cut(s) 250
PciI ACATGT 1 cut(s) 96
PciSI GCTCTTC 1 cut(s) 264
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PfeI GAWTC 1 cut(s) 278
PkrI GCNGC 1 cut(s) 142
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PscI ACATGT 1 cut(s) 96
Psp124BI GAGCTC 2 cut(s) 72, 81
Psp6I CCWGG 1 cut(s) 168
PspGI CCWGG 1 cut(s) 168
PspPI GGNCC 2 cut(s) 166, 181
PsuI RGATCY 1 cut(s) 60
RruI TCGCGA 1 cut(s) 192
RseI CAYNNNNRTG 1 cut(s) 250
SacI GAGCTC 2 cut(s) 72, 81
SapI GCTCTTC 1 cut(s) 264
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 2 cut(s) 39, 60
Sau96I GGNCC 2 cut(s) 166, 181
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 3 cut(s) 46, 170, 185
SduI GDGCHC 2 cut(s) 72, 81
SetI ASST 5 cut(s) 72, 81, 162, 168, 276
SfaNI GCATC 2 cut(s) 32, 127
SinI GGWCC 2 cut(s) 166, 181
SmiMI CAYNNNNRTG 1 cut(s) 250
SsiI CCGC 2 cut(s) 8, 141
SstI GAGCTC 2 cut(s) 72, 81
StyD4I CCNGG 3 cut(s) 44, 168, 183
StyI CCWWGG 1 cut(s) 153
TaaI ACNGT 2 cut(s) 56, 115
TaqI TCGA 1 cut(s) 135
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 1 cut(s) 278
VpaK11BI GGWCC 2 cut(s) 166, 181
XceI RCATGY 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.