Rh5AG203700

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001324
Physical Location & Seq
Reverse (-)
23953466 .. 23956677
3212 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG203700.1

Sequence Viewer

Length: 822 bp
ATGGAGGCGGAGAAAGCAGGGGCGTCGTTGGACTCGCTGATCGCCGGGTTCAACAGTCGGATCTCGGAGCTCAAAGAACTCGTTATTGCGCGAAACATGTACCCAGCCAGCAGCGTCACCGATTTGTCGGCCGTCGACGCCGCCGTCAAGGCCATGGAGCTTCAGGTCCAGGCCATCAAGGACCGGGTTGGCGACGAAACCCTAGCCATCCCCAAAGCAAAAAAACTGATCGATGCATCGCTGAAGCAGCAAAGGAAATTGAAGGATATGTCAGTTTATGCCCCCGCTCATGTTCCTGAAAGGATGTCAATGTTGAATTTGGATACAAACAGATGTTTATTTCCAGAAAGCTCTCAGCAAAACACTGGTTCTGGGACCTTCAATCTTGACGAGGATCCTGCACCACCACCACAACCACCACCAAAGGAGAAAAAGGGTCGTTCCACACCACCATTGTGGTTTATAAGTACTGAAGAGTTGGATTCCTTGTCATCATACATGAGAGGAAGGCTTACATTAGAGAAGGTCAATGCAGCTATTAATGACATGGCAACATATGCTGAAGCAAATTATCAGCTCATAGCTGCTCCAAAGAAGAATGTGGCAGGGGATCGATGGGAAAAAGCCTTGGAAATAAGAGATATAGCAATGCATGAAGCAGTGAAGGGAAAACACTTCTTTCTCGAAACTGACATGAAGGGACCATCCTTGAAGCTTGACAACACTGGAAAAGCAATACTAACTGTCCTTCGTCACCTTGGTCGGGTAAATGAGACACGGATTGGTCATCATCGGGTGATCATTCTTTTGAAGCCTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

273

Amino Acids

30.23

Weight (kDa)

8.79

Isoelectric Point (pI)

43.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 9 - 263 1.1e-85 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 464
AasI GACNNNNNNGTC 1 cut(s) 143
AccBSI CCGCTC 1 cut(s) 287
AccI GTMKAC 1 cut(s) 135
AccII CGCG 1 cut(s) 91
AciI CCGC 3 cut(s) 8, 141, 285
AclWI GGATC 4 cut(s) 68, 389, 402, 618
AcoI YGGCCR 1 cut(s) 129
AcsI RAATTY 1 cut(s) 316
AcuI CTGAAG 4 cut(s) 146, 263, 492, 582
AcyI GRCGYC 2 cut(s) 23, 138
AfaI GTAC 2 cut(s) 101, 469
AfiI CCNNNNNNNGG 1 cut(s) 763
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 6 cut(s) 52, 262, 316, 382, 712, 811
AjnI CCWGG 1 cut(s) 168
AleI CACNNNNGTG 1 cut(s) 454
AluBI AGCT 7 cut(s) 70, 160, 351, 536, 577, 584, 715
AluI AGCT 7 cut(s) 70, 160, 351, 536, 577, 584, 715
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 1 cut(s) 767
AlwI GGATC 4 cut(s) 68, 389, 402, 618
AoxI GGCC 3 cut(s) 129, 150, 171
ApeKI GCWGC 4 cut(s) 111, 247, 533, 584
ApoI RAATTY 1 cut(s) 316
AseI ATTAAT 1 cut(s) 540
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 4 cut(s) 166, 181, 375, 701
AsuC2I CCSGG 2 cut(s) 46, 185
AsuHPI GGTGA 3 cut(s) 109, 746, 808
AvaII GGWCC 4 cut(s) 166, 181, 375, 701
BamHI GGATCC 1 cut(s) 394
BanII GRGCYC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 72
BbvI GCAGC 4 cut(s) 123, 259, 545, 571
BccI CCATC 4 cut(s) 182, 215, 609, 712
BceAI ACGGC 2 cut(s) 116, 128
BcgI CGANNNNNNTGC 4 cut(s) 6, 40, 380, 414
BciT130I CCWGG 1 cut(s) 170
BciVI GTATCC 1 cut(s) 316
BclI TGATCA 1 cut(s) 798
BcnI CCSGG 2 cut(s) 46, 185
BcoDI GTCTC 1 cut(s) 767
BfaI CTAG 1 cut(s) 203
BfuI GTATCC 1 cut(s) 316
BglI GCCNNNNNGGC 1 cut(s) 149
BisI GCNGC 5 cut(s) 112, 141, 248, 534, 585
BlsI GCNGC 5 cut(s) 113, 142, 249, 535, 586
BmcAI AGTACT 1 cut(s) 469
Bme1390I CCNGG 3 cut(s) 46, 170, 185
Bme18I GGWCC 4 cut(s) 166, 181, 375, 701
BmgT120I GGNCC 4 cut(s) 166, 181, 375, 701
BmiI GGNNCC 3 cut(s) 376, 396, 702
BmrFI CCNGG 3 cut(s) 46, 170, 185
BmsI GCATC 2 cut(s) 223, 245
BpuMI CCSGG 2 cut(s) 46, 185
Bsa29I ATCGAT 2 cut(s) 231, 613
BsaHI GRCGYC 2 cut(s) 23, 138
BsaJI CCNNGG 3 cut(s) 153, 627, 757
Bsc4I CCNNNNNNNGG 1 cut(s) 763
Bse1I ACTGG 2 cut(s) 370, 730
Bse3DI GCAATG 1 cut(s) 654
BseBI CCWGG 1 cut(s) 170
BseCI ATCGAT 2 cut(s) 231, 613
BseDI CCNNGG 3 cut(s) 153, 627, 757
BseGI GGATG 3 cut(s) 207, 309, 704
BseLI CCNNNNNNNGG 1 cut(s) 763
BseMI GCAATG 1 cut(s) 654
BseMII CTCAG 1 cut(s) 368
BseNI ACTGG 2 cut(s) 370, 730
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 4 cut(s) 123, 259, 545, 571
BseYI CCCAGC 1 cut(s) 103
BsgI GTGCAG 1 cut(s) 384
Bsh1236I CGCG 1 cut(s) 91
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 3 cut(s) 131, 152, 173
BshVI ATCGAT 2 cut(s) 231, 613
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 72
BsiSI CCGG 2 cut(s) 45, 184
BslFI GGGAC 2 cut(s) 388, 714
BslI CCNNNNNNNGG 1 cut(s) 763
BsmAI GTCTC 1 cut(s) 767
BsmFI GGGAC 2 cut(s) 388, 714
BsnI GGCC 3 cut(s) 131, 152, 173
Bsp1286I GDGCHC 1 cut(s) 72
Bsp143I GATC 6 cut(s) 39, 60, 228, 394, 610, 798
Bsp19I CCATGG 1 cut(s) 153
BspACI CCGC 3 cut(s) 8, 141, 285
BspANI GGCC 3 cut(s) 131, 152, 173
BspCNI CTCAG 1 cut(s) 367
BspDI ATCGAT 2 cut(s) 231, 613
BspFNI CGCG 1 cut(s) 91
BspLI GGNNCC 3 cut(s) 376, 396, 702
BspPI GGATC 4 cut(s) 68, 389, 402, 618
BsrBI CCGCTC 1 cut(s) 287
BsrDI GCAATG 1 cut(s) 654
BsrI ACTGG 2 cut(s) 370, 730
BssECI CCNNGG 3 cut(s) 153, 627, 757
BssMI GATC 6 cut(s) 39, 60, 228, 394, 610, 798
BssNI GRCGYC 2 cut(s) 23, 138
BssT1I CCWWGG 3 cut(s) 153, 627, 757
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 2 cut(s) 56, 745
Bst6I CTCTTC 1 cut(s) 468
BstACI GRCGYC 2 cut(s) 23, 138
BstAPI GCANNNNNTGC 1 cut(s) 557
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 1 cut(s) 354
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 3 cut(s) 207, 309, 704
BstFNI CGCG 1 cut(s) 91
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 6 cut(s) 42, 63, 231, 397, 613, 801
BstMAI GTCTC 1 cut(s) 767
BstMBI GATC 6 cut(s) 39, 60, 228, 394, 610, 798
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 5 cut(s) 14, 137, 149, 247, 557
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 100
BstSCI CCNGG 3 cut(s) 44, 168, 183
BstUI CGCG 1 cut(s) 91
BstV1I GCAGC 4 cut(s) 123, 259, 545, 571
BstX2I RGATCY 2 cut(s) 60, 394
BstXI CCANNNNNNTGG 1 cut(s) 456
BstYI RGATCY 2 cut(s) 60, 394
BstZI CGGCCG 1 cut(s) 129
Bsu15I ATCGAT 2 cut(s) 231, 613
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 3 cut(s) 131, 152, 173
BsuTUI ATCGAT 2 cut(s) 231, 613
BtgI CCRYGG 1 cut(s) 153
BtgZI GCGATG 1 cut(s) 222
BtsCI GGATG 3 cut(s) 207, 309, 704
BtsI GCAGTG 1 cut(s) 666
BtsIMutI CAGTG 4 cut(s) 363, 666, 723, 817
Cac8I GCNNGC 1 cut(s) 109
CfoI GCGC 1 cut(s) 91
Cfr13I GGNCC 4 cut(s) 166, 181, 375, 701
ClaI ATCGAT 2 cut(s) 231, 613
CseI GACGC 3 cut(s) 12, 103, 146
Csp6I GTAC 2 cut(s) 100, 468
CviAII CATG 7 cut(s) 97, 154, 290, 499, 547, 653, 694
CviQI GTAC 2 cut(s) 100, 468
DdeI CTNAG 1 cut(s) 354
DpnI GATC 6 cut(s) 41, 62, 230, 396, 612, 800
DpnII GATC 6 cut(s) 39, 60, 228, 394, 610, 798
DrdI GACNNNNNNGTC 1 cut(s) 143
DseDI GACNNNNNNGTC 1 cut(s) 143
EaeI YGGCCR 1 cut(s) 129
EagI CGGCCG 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 468
EarI CTCTTC 1 cut(s) 468
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 1 cut(s) 70
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 3 cut(s) 153, 627, 757
Eco24I GRGCYC 1 cut(s) 72
Eco47I GGWCC 4 cut(s) 166, 181, 375, 701
Eco52I CGGCCG 1 cut(s) 129
Eco53kI GAGCTC 1 cut(s) 70
Eco57I CTGAAG 4 cut(s) 146, 263, 492, 582
EcoICRI GAGCTC 1 cut(s) 70
EcoO109I RGGNCCY 1 cut(s) 375
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 3 cut(s) 153, 627, 757
EcoT22I ATGCAT 2 cut(s) 238, 654
EcoT38I GRGCYC 1 cut(s) 72
ErhI CCWWGG 3 cut(s) 153, 627, 757
FaeI CATG 7 cut(s) 100, 157, 293, 502, 550, 656, 697
FaqI GGGAC 2 cut(s) 388, 714
FatI CATG 7 cut(s) 96, 153, 289, 498, 546, 652, 693
FauI CCCGC 1 cut(s) 292
FauNDI CATATG 1 cut(s) 556
FbaI TGATCA 1 cut(s) 798
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 5 cut(s) 112, 141, 248, 534, 585
FokI GGATG 3 cut(s) 194, 316, 691
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 5 cut(s) 112, 141, 248, 534, 585
FspBI CTAG 1 cut(s) 203
GlaI GCGC 1 cut(s) 90
GluI GCNGC 5 cut(s) 112, 141, 248, 534, 585
GsaI CCCAGC 1 cut(s) 107
HaeIII GGCC 3 cut(s) 131, 152, 173
HapII CCGG 2 cut(s) 45, 184
HgaI GACGC 3 cut(s) 12, 103, 146
HhaI GCGC 1 cut(s) 91
Hin1I GRCGYC 2 cut(s) 23, 138
Hin1II CATG 7 cut(s) 100, 157, 293, 502, 550, 656, 697
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HindIII AAGCTT 1 cut(s) 713
HinfI GANTC 2 cut(s) 32, 482
HpaII CCGG 2 cut(s) 45, 184
HphI GGTGA 3 cut(s) 109, 746, 808
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 2 cut(s) 60, 67
Hpy188III TCNNGA 4 cut(s) 296, 344, 386, 683
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 4 cut(s) 28, 137, 140, 197
HpyAV CCTTC 7 cut(s) 256, 388, 501, 517, 658, 691, 758
HpyCH4III ACNGT 2 cut(s) 56, 745
HpyCH4V TGCA 4 cut(s) 236, 401, 533, 652
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 137, 149, 247, 557
HpyF3I CTNAG 1 cut(s) 354
Hsp92I GRCGYC 2 cut(s) 23, 138
Hsp92II CATG 7 cut(s) 100, 157, 293, 502, 550, 656, 697
HspAI GCGC 1 cut(s) 89
Ksp22I TGATCA 1 cut(s) 798
Kzo9I GATC 6 cut(s) 39, 60, 228, 394, 610, 798
LmnI GCTCC 3 cut(s) 67, 157, 592
Lsp1109I GCAGC 4 cut(s) 123, 259, 545, 571
LweI GCATC 2 cut(s) 223, 245
MaeI CTAG 1 cut(s) 203
MaeIII GTNAC 2 cut(s) 115, 752
MalI GATC 6 cut(s) 41, 62, 230, 396, 612, 800
MbiI CCGCTC 1 cut(s) 287
MboI GATC 6 cut(s) 39, 60, 228, 394, 610, 798
MboII GAAGA 2 cut(s) 485, 607
MflI RGATCY 2 cut(s) 60, 394
MhlI GDGCHC 1 cut(s) 72
MluCI AATT 3 cut(s) 257, 316, 568
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 3 cut(s) 9, 38, 459
MnlI CCTC 2 cut(s) 385, 497
Mph1103I ATGCAT 2 cut(s) 238, 654
MseI TTAA 1 cut(s) 540
MslI CAYNNNNRTG 1 cut(s) 454
MspI CCGG 2 cut(s) 45, 184
MspR9I CCNGG 3 cut(s) 46, 170, 185
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 1 cut(s) 91
MwoI GCNNNNNNNGC 5 cut(s) 14, 137, 149, 247, 557
NciI CCSGG 2 cut(s) 46, 185
NcoI CCATGG 1 cut(s) 153
NdeI CATATG 1 cut(s) 556
NdeII GATC 6 cut(s) 39, 60, 228, 394, 610, 798
NlaIII CATG 7 cut(s) 100, 157, 293, 502, 550, 656, 697
NlaIV GGNNCC 3 cut(s) 376, 396, 702
NmuCI GTSAC 2 cut(s) 115, 752
NsiI ATGCAT 2 cut(s) 238, 654
NspI RCATGY 1 cut(s) 100
OliI CACNNNNGTG 1 cut(s) 454
PciI ACATGT 1 cut(s) 96
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PfeI GAWTC 1 cut(s) 482
PkrI GCNGC 5 cut(s) 113, 142, 249, 535, 586
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PpuMI RGGWCCY 1 cut(s) 375
PscI ACATGT 1 cut(s) 96
PshBI ATTAAT 1 cut(s) 540
PsiI TTATAA 1 cut(s) 464
Psp124BI GAGCTC 1 cut(s) 72
Psp5II RGGWCCY 1 cut(s) 375
Psp6I CCWGG 1 cut(s) 168
PspFI CCCAGC 1 cut(s) 103
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 3 cut(s) 376, 396, 702
PspPI GGNCC 4 cut(s) 166, 181, 375, 701
PspPPI RGGWCCY 1 cut(s) 375
PsuI RGATCY 2 cut(s) 60, 394
RsaI GTAC 2 cut(s) 101, 469
RsaNI GTAC 2 cut(s) 100, 468
RseI CAYNNNNRTG 1 cut(s) 454
SacI GAGCTC 1 cut(s) 72
SalI GTCGAC 1 cut(s) 134
SaqAI TTAA 1 cut(s) 540
SatI GCNGC 5 cut(s) 112, 141, 248, 534, 585
Sau3AI GATC 6 cut(s) 39, 60, 228, 394, 610, 798
Sau96I GGNCC 4 cut(s) 166, 181, 375, 701
ScaI AGTACT 1 cut(s) 469
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 3 cut(s) 46, 170, 185
SduI GDGCHC 1 cut(s) 72
SfaNI GCATC 2 cut(s) 223, 245
SgrDI CGTCGACG 1 cut(s) 134
SinI GGWCC 4 cut(s) 166, 181, 375, 701
SmiMI CAYNNNNRTG 1 cut(s) 454
Sse9I AATT 3 cut(s) 257, 316, 568
SsiI CCGC 3 cut(s) 8, 141, 285
SspMI CTAG 1 cut(s) 203
SstI GAGCTC 1 cut(s) 72
StyD4I CCNGG 3 cut(s) 44, 168, 183
StyI CCWWGG 3 cut(s) 153, 627, 757
TaaI ACNGT 2 cut(s) 56, 745
TaqI TCGA 4 cut(s) 135, 231, 613, 684
TasI AATT 3 cut(s) 257, 316, 568
TatI WGTACW 1 cut(s) 467
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 1 cut(s) 482
Tru1I TTAA 1 cut(s) 540
Tru9I TTAA 1 cut(s) 540
TscAI CASTG 3 cut(s) 370, 666, 730
TseFI GTSAC 2 cut(s) 115, 752
TseI GCWGC 4 cut(s) 111, 247, 533, 584
Tsp45I GTSAC 2 cut(s) 115, 752
TspDTI ATGAA 2 cut(s) 669, 710
TspGWI ACGGA 1 cut(s) 793
TspRI CASTG 3 cut(s) 370, 666, 730
VpaK11BI GGWCC 4 cut(s) 166, 181, 375, 701
VspI ATTAAT 1 cut(s) 540
XapI RAATTY 1 cut(s) 316
XceI RCATGY 1 cut(s) 100
XmiI GTMKAC 1 cut(s) 135
XspI CTAG 1 cut(s) 203
ZrmI AGTACT 1 cut(s) 469
Zsp2I ATGCAT 2 cut(s) 238, 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.