Prupe.2G138600_v2.0.a1

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0001324
Physical Location & Seq
Forward (+)
19563708 .. 19567777
4070 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G138600.1

Sequence Viewer

Length: 810 bp
ATGGAGGTGAAGAAAGCAGGGGCGTCGTTGGATTCGGTGATCTCATTGTTCAACCGTCGGATCGCGGAGCTTCAAGACCTCGTCATTGCGCGAAACATGTATCCGGCGACCAGCGTCACCGATTTGTCGGCCGTTGACGCCGCAGTGAAGACCATGGAGCTTCAGGTTCAGGCCATCAAGGACCGCCTACGCGACGAAACCCTAGCCATCCCCAAAGCCAAAAAACTCATCGATGCGGCGGTGAAGCAGCAAAAGAAATTGCAGGACCTGTCAAGTTATGCGCCCTCACATATTCCTGAAAGAATGTCTATGTTAGGTTTGGATACTAACAGATGTTTATTCCCAGAAGGCTCTCAACAAAACACCGGTTTCGGGTCGTTGAAGCTTGAGGAGGTGCCTGCAGCACTACCTAAGGAGAAAAAGGGTCGTTCCACTCCACCACTGTGGTTCATAACTGCTGAAGAGCTGGATTCCCTGTCAGGATACATGAGAGGAAGGCTTACTGTAGAAAAGATCAATGCAGCTATTAATGACATGGCAACATATGCTGAAGCAAATTCTCAGCTTATATCTGCCCCGAAGAAGAGGGTGGCAGGAGATAGTTGGGAAAAGGCCCTGGAACTGAGAGATATTGCAATGACAGAAGCAGTAAAGGGAAAACACTTCTTTCTCGAAACTGACATGAAGGGACCAGCCTTGAAGCCTGACAACACTGGAAAAGCAATACTAACTGTCCTTCGTCACCTTGGTCGTGTAAGCGAGACACGGATTGGGCCTCATCGAGTGATCATTCTTTTGAAGCGTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

270

Amino Acids

29.76

Weight (kDa)

9.46

Isoelectric Point (pI)

44.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 394
AccII CGCG 3 cut(s) 65, 91, 192
AciI CCGC 5 cut(s) 65, 141, 184, 236, 239
AclWI GGATC 1 cut(s) 68
AcoI YGGCCR 1 cut(s) 129
AcsI RAATTY 1 cut(s) 556
AcuI CTGAAG 3 cut(s) 146, 480, 570
AcyI GRCGYC 2 cut(s) 23, 138
AfiI CCNNNNNNNGG 1 cut(s) 372
AflIII ACRYGT 1 cut(s) 96
AgeI ACCGGT 1 cut(s) 365
AgsI TTSAA 5 cut(s) 52, 74, 382, 700, 799
AjnI CCWGG 1 cut(s) 615
AleI CACNNNNGTG 1 cut(s) 442
AluBI AGCT 6 cut(s) 70, 160, 385, 466, 524, 565
AluI AGCT 6 cut(s) 70, 160, 385, 466, 524, 565
Alw26I GTCTC 1 cut(s) 755
AlwI GGATC 1 cut(s) 68
AlwNI CAGNNNCTG 1 cut(s) 268
AoxI GGCC 4 cut(s) 129, 171, 612, 773
ApeKI GCWGC 3 cut(s) 247, 401, 521
ApoI RAATTY 1 cut(s) 556
AseI ATTAAT 1 cut(s) 528
AsiGI ACCGGT 1 cut(s) 365
AspLEI GCGC 2 cut(s) 91, 283
AspS9I GGNCC 5 cut(s) 181, 265, 613, 689, 773
AsuHPI GGTGA 5 cut(s) 19, 49, 109, 253, 734
AvaII GGWCC 3 cut(s) 181, 265, 689
AxyI CCTNAGG 1 cut(s) 411
BanI GGYRCC 1 cut(s) 394
BbsI GAAGAC 1 cut(s) 155
BbvI GCAGC 3 cut(s) 259, 413, 533
BccI CCATC 2 cut(s) 182, 215
BceAI ACGGC 1 cut(s) 116
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BciT130I CCWGG 1 cut(s) 617
BciVI GTATCC 3 cut(s) 111, 316, 476
BclI TGATCA 1 cut(s) 786
BcoDI GTCTC 1 cut(s) 755
BfaI CTAG 1 cut(s) 203
BfmI CTRYAG 2 cut(s) 399, 504
BfuI GTATCC 3 cut(s) 111, 316, 476
BisI GCNGC 5 cut(s) 141, 237, 248, 402, 522
BlsI GCNGC 5 cut(s) 142, 238, 249, 403, 523
Bme1390I CCNGG 1 cut(s) 617
Bme18I GGWCC 3 cut(s) 181, 265, 689
BmgT120I GGNCC 5 cut(s) 181, 265, 613, 689, 773
BmiI GGNNCC 2 cut(s) 396, 690
BmrFI CCNGG 1 cut(s) 617
BmsI GCATC 1 cut(s) 223
BoxI GACNNNNGTC 1 cut(s) 113
BpiI GAAGAC 1 cut(s) 155
BpuEI CTTGAG 1 cut(s) 407
Bsa29I ATCGAT 1 cut(s) 231
BsaHI GRCGYC 2 cut(s) 23, 138
BsaJI CCNNGG 3 cut(s) 153, 615, 745
BsaWI WCCGGW 1 cut(s) 365
Bsc4I CCNNNNNNNGG 1 cut(s) 372
Bse118I RCCGGY 1 cut(s) 365
Bse1I ACTGG 1 cut(s) 718
Bse21I CCTNAGG 1 cut(s) 411
Bse3DI GCAATG 2 cut(s) 84, 642
BseBI CCWGG 1 cut(s) 617
BseCI ATCGAT 1 cut(s) 231
BseDI CCNNGG 3 cut(s) 153, 615, 745
BseGI GGATG 1 cut(s) 207
BseLI CCNNNNNNNGG 1 cut(s) 372
BseMI GCAATG 2 cut(s) 84, 642
BseMII CTCAG 2 cut(s) 575, 614
BseNI ACTGG 1 cut(s) 718
BseRI GAGGAG 1 cut(s) 404
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 3 cut(s) 259, 413, 533
Bsh1236I CGCG 3 cut(s) 65, 91, 192
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 4 cut(s) 131, 173, 614, 775
BshNI GGYRCC 1 cut(s) 394
BshTI ACCGGT 1 cut(s) 365
BshVI ATCGAT 1 cut(s) 231
BsiEI CGRYCG 1 cut(s) 132
BsiSI CCGG 2 cut(s) 104, 366
BslFI GGGAC 1 cut(s) 702
BslI CCNNNNNNNGG 1 cut(s) 372
BsmAI GTCTC 1 cut(s) 755
BsmFI GGGAC 1 cut(s) 702
BsnI GGCC 4 cut(s) 131, 173, 614, 775
Bsp143I GATC 4 cut(s) 39, 60, 513, 786
Bsp19I CCATGG 1 cut(s) 153
BspACI CCGC 5 cut(s) 65, 141, 184, 236, 239
BspANI GGCC 4 cut(s) 131, 173, 614, 775
BspCNI CTCAG 2 cut(s) 574, 615
BspDI ATCGAT 1 cut(s) 231
BspFNI CGCG 3 cut(s) 65, 91, 192
BspLI GGNNCC 2 cut(s) 396, 690
BspMAI CTGCAG 1 cut(s) 403
BspPI GGATC 1 cut(s) 68
BspQI GCTCTTC 1 cut(s) 456
BspT107I GGYRCC 1 cut(s) 394
BsrDI GCAATG 2 cut(s) 84, 642
BsrFI RCCGGY 1 cut(s) 365
BsrI ACTGG 1 cut(s) 718
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 3 cut(s) 153, 615, 745
BssMI GATC 4 cut(s) 39, 60, 513, 786
BssNI GRCGYC 2 cut(s) 23, 138
BssT1I CCWWGG 2 cut(s) 153, 745
Bst2UI CCWGG 1 cut(s) 617
Bst4CI ACNGT 4 cut(s) 56, 444, 505, 733
Bst6I CTCTTC 2 cut(s) 456, 578
BstACI GRCGYC 2 cut(s) 23, 138
BstAPI GCANNNNNTGC 1 cut(s) 545
BstC8I GCNNGC 1 cut(s) 399
BstDEI CTNAG 3 cut(s) 411, 561, 623
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 1 cut(s) 207
BstFNI CGCG 3 cut(s) 65, 91, 192
BstHHI GCGC 2 cut(s) 91, 283
BstKTI GATC 4 cut(s) 42, 63, 516, 789
BstMAI GTCTC 1 cut(s) 755
BstMBI GATC 4 cut(s) 39, 60, 513, 786
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 2 cut(s) 137, 545
BstNI CCWGG 1 cut(s) 617
BstNSI RCATGY 1 cut(s) 100
BstPAI GACNNNNGTC 1 cut(s) 113
BstSCI CCNGG 1 cut(s) 615
BstSFI CTRYAG 2 cut(s) 399, 504
BstUI CGCG 3 cut(s) 65, 91, 192
BstV1I GCAGC 3 cut(s) 259, 413, 533
BstV2I GAAGAC 1 cut(s) 155
BstXI CCANNNNNNTGG 1 cut(s) 444
BstZI CGGCCG 1 cut(s) 129
Bsu15I ATCGAT 1 cut(s) 231
Bsu36I CCTNAGG 1 cut(s) 411
BsuI GTATCC 3 cut(s) 111, 316, 476
BsuRI GGCC 4 cut(s) 131, 173, 614, 775
BsuTUI ATCGAT 1 cut(s) 231
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 1 cut(s) 207
BtsI GCAGTG 1 cut(s) 150
BtsIMutI CAGTG 3 cut(s) 150, 440, 711
Cac8I GCNNGC 1 cut(s) 399
CaiI CAGNNNCTG 1 cut(s) 268
CfoI GCGC 2 cut(s) 91, 283
Cfr10I RCCGGY 1 cut(s) 365
Cfr13I GGNCC 5 cut(s) 181, 265, 613, 689, 773
ClaI ATCGAT 1 cut(s) 231
CseI GACGC 4 cut(s) 12, 103, 146, 791
CspAI ACCGGT 1 cut(s) 365
CviAII CATG 5 cut(s) 97, 154, 487, 535, 682
DdeI CTNAG 3 cut(s) 411, 561, 623
DpnI GATC 4 cut(s) 41, 62, 515, 788
DpnII GATC 4 cut(s) 39, 60, 513, 786
EaeI YGGCCR 1 cut(s) 129
EagI CGGCCG 1 cut(s) 129
Eam1104I CTCTTC 2 cut(s) 456, 578
EarI CTCTTC 2 cut(s) 456, 578
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 2 cut(s) 153, 745
Eco47I GGWCC 3 cut(s) 181, 265, 689
Eco52I CGGCCG 1 cut(s) 129
Eco57I CTGAAG 3 cut(s) 146, 480, 570
Eco81I CCTNAGG 1 cut(s) 411
EcoO109I RGGNCCY 2 cut(s) 265, 613
EcoRII CCWGG 1 cut(s) 615
EcoT14I CCWWGG 2 cut(s) 153, 745
ErhI CCWWGG 2 cut(s) 153, 745
FaeI CATG 5 cut(s) 100, 157, 490, 538, 685
FaqI GGGAC 1 cut(s) 702
FatI CATG 5 cut(s) 96, 153, 486, 534, 681
FauNDI CATATG 1 cut(s) 544
FbaI TGATCA 1 cut(s) 786
Fnu4HI GCNGC 5 cut(s) 141, 237, 248, 402, 522
FokI GGATG 1 cut(s) 194
Fsp4HI GCNGC 5 cut(s) 141, 237, 248, 402, 522
FspBI CTAG 1 cut(s) 203
GlaI GCGC 2 cut(s) 90, 282
GluI GCNGC 5 cut(s) 141, 237, 248, 402, 522
HaeIII GGCC 4 cut(s) 131, 173, 614, 775
HapII CCGG 2 cut(s) 104, 366
HgaI GACGC 4 cut(s) 12, 103, 146, 791
HhaI GCGC 2 cut(s) 91, 283
Hin1I GRCGYC 2 cut(s) 23, 138
Hin1II CATG 5 cut(s) 100, 157, 490, 538, 685
Hin6I GCGC 2 cut(s) 89, 281
HinP1I GCGC 2 cut(s) 89, 281
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 2 cut(s) 32, 470
HpaII CCGG 2 cut(s) 104, 366
HphI GGTGA 5 cut(s) 19, 49, 109, 253, 734
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 1 cut(s) 60
Hpy188III TCNNGA 4 cut(s) 74, 296, 480, 671
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 3 cut(s) 28, 60, 197
HpyAV CCTTC 4 cut(s) 341, 489, 679, 746
HpyCH4III ACNGT 4 cut(s) 56, 444, 505, 733
HpyCH4V TGCA 4 cut(s) 262, 401, 521, 635
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 545
HpyF3I CTNAG 3 cut(s) 411, 561, 623
Hsp92I GRCGYC 2 cut(s) 23, 138
Hsp92II CATG 5 cut(s) 100, 157, 490, 538, 685
HspAI GCGC 2 cut(s) 89, 281
Ksp22I TGATCA 1 cut(s) 786
Kzo9I GATC 4 cut(s) 39, 60, 513, 786
LguI GCTCTTC 1 cut(s) 456
LmnI GCTCC 2 cut(s) 67, 157
Lsp1109I GCAGC 3 cut(s) 259, 413, 533
LweI GCATC 1 cut(s) 223
MaeI CTAG 1 cut(s) 203
MaeIII GTNAC 2 cut(s) 115, 740
MalI GATC 4 cut(s) 41, 62, 515, 788
MboI GATC 4 cut(s) 39, 60, 513, 786
MboII GAAGA 5 cut(s) 22, 160, 473, 592, 595
MluCI AATT 2 cut(s) 257, 556
MmeI TCCRAC 2 cut(s) 9, 38
MnlI CCTC 7 cut(s) 89, 295, 382, 385, 485, 579, 786
MseI TTAA 1 cut(s) 528
MslI CAYNNNNRTG 1 cut(s) 442
MspI CCGG 2 cut(s) 104, 366
MspR9I CCNGG 1 cut(s) 617
MvaI CCWGG 1 cut(s) 617
MvnI CGCG 3 cut(s) 65, 91, 192
MwoI GCNNNNNNNGC 2 cut(s) 137, 545
NcoI CCATGG 1 cut(s) 153
NdeI CATATG 1 cut(s) 544
NdeII GATC 4 cut(s) 39, 60, 513, 786
NlaIII CATG 5 cut(s) 100, 157, 490, 538, 685
NlaIV GGNNCC 2 cut(s) 396, 690
NmuCI GTSAC 2 cut(s) 115, 740
NspI RCATGY 1 cut(s) 100
OliI CACNNNNGTG 1 cut(s) 442
PciI ACATGT 1 cut(s) 96
PciSI GCTCTTC 1 cut(s) 456
PfeI GAWTC 2 cut(s) 32, 470
PflFI GACNNNGTC 1 cut(s) 80
PinAI ACCGGT 1 cut(s) 365
PkrI GCNGC 5 cut(s) 142, 238, 249, 403, 523
PpuMI RGGWCCY 1 cut(s) 265
PscI ACATGT 1 cut(s) 96
PshAI GACNNNNGTC 1 cut(s) 113
PshBI ATTAAT 1 cut(s) 528
Psp5II RGGWCCY 1 cut(s) 265
Psp6I CCWGG 1 cut(s) 615
PspGI CCWGG 1 cut(s) 615
PspN4I GGNNCC 2 cut(s) 396, 690
PspPI GGNCC 5 cut(s) 181, 265, 613, 689, 773
PspPPI RGGWCCY 1 cut(s) 265
PstI CTGCAG 1 cut(s) 403
PstNI CAGNNNCTG 1 cut(s) 268
PsyI GACNNNGTC 1 cut(s) 80
RseI CAYNNNNRTG 1 cut(s) 442
SapI GCTCTTC 1 cut(s) 456
SaqAI TTAA 1 cut(s) 528
SatI GCNGC 5 cut(s) 141, 237, 248, 402, 522
Sau3AI GATC 4 cut(s) 39, 60, 513, 786
Sau96I GGNCC 5 cut(s) 181, 265, 613, 689, 773
ScrFI CCNGG 1 cut(s) 617
SfaNI GCATC 1 cut(s) 223
SfcI CTRYAG 2 cut(s) 399, 504
SinI GGWCC 3 cut(s) 181, 265, 689
SmiMI CAYNNNNRTG 1 cut(s) 442
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
Sse9I AATT 2 cut(s) 257, 556
SsiI CCGC 5 cut(s) 65, 141, 184, 236, 239
SspMI CTAG 1 cut(s) 203
StyD4I CCNGG 1 cut(s) 615
StyI CCWWGG 2 cut(s) 153, 745
TaaI ACNGT 4 cut(s) 56, 444, 505, 733
TaqI TCGA 3 cut(s) 231, 672, 781
TasI AATT 2 cut(s) 257, 556
TauI GCSGC 2 cut(s) 143, 239
TfiI GAWTC 2 cut(s) 32, 470
Tru1I TTAA 1 cut(s) 528
Tru9I TTAA 1 cut(s) 528
TscAI CASTG 3 cut(s) 150, 447, 718
TseFI GTSAC 2 cut(s) 115, 740
TseI GCWGC 3 cut(s) 247, 401, 521
Tsp45I GTSAC 2 cut(s) 115, 740
TspDTI ATGAA 2 cut(s) 439, 698
TspGWI ACGGA 1 cut(s) 781
TspRI CASTG 3 cut(s) 150, 447, 718
Tth111I GACNNNGTC 1 cut(s) 80
VpaK11BI GGWCC 3 cut(s) 181, 265, 689
VspI ATTAAT 1 cut(s) 528
XapI RAATTY 1 cut(s) 556
XceI RCATGY 1 cut(s) 100
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.