Rroxscaffold_4G00305890

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001324
Physical Location & Seq
Reverse (-)
26626182 .. 26629906
3725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00305890.1

Sequence Viewer

Length: 819 bp
ATGGAGGCAGAGAAAGCAGGGGCGTCGTTGGACTCGCTGATCGCCGGGTTCAACAGTCGGGTCTCGGAGCTCCAAGGGCTTGTTATTGCGCGAAACATGTACCCGGCCAGCAGCGTCACCGATTTGACGGCCGTCGACGCAGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGCCATCAAGGACCGGGTTCGCGACGAAACCCTAGCTATCCCCAAAGCAAAAAAACTGATCGATGCATTGCTGAAGCAACAAAGGAAATTGAAGGATATGTCAGTTTATGCCCCCACTCATGTTCCGGAAAGGATGTCAATGTTGAATCTGGATACAAACAGATGGTATTTATTTCCAGAAAGCTCTCAGCAAAACAATGGTTCTGGGCCTTTCAAGCTTGACGAGGATCCTGCACCACCACCACCACCTAAGGAGAAAAAGGGTCGTTCCACCCCACCATTGTGGTTTATTAGTGCTGAAGAGCTGGATTCCTTGTCATCATACATGAGAGGAAGGCTTACACTAGAGAAGGTCAATGCAGCTATTAATGACATGGTAACATATGTTGAAACAAATTATCAGCTCATAGCTGCTCCAAAGAAAAATGTGGCAGGAGATCGATGGGAAAAAGCCTTGGAAATAAGAGATATAGCAATGCATGAAGCAGTAAAGGGAAAACACTTCTTTCTCGAAACTGACATGAAAGGACCATCCTTGAAGCTTGACAACACTGGAAAAGCAATACTAACTGTCCTTCGTCACCTTGATCGTGTAAGTGAGACAAGAATTGGTCATCATCGGGTGATCATTCTTTTGAAGCCTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

272

Amino Acids

30.45

Weight (kDa)

9.06

Isoelectric Point (pI)

42.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 9 - 262 1.8e-81 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 135
AccII CGCG 2 cut(s) 91, 192
AccIII TCCGGA 1 cut(s) 295
AclWI GGATC 2 cut(s) 392, 405
AcoI YGGCCR 2 cut(s) 105, 129
AcuI CTGAAG 3 cut(s) 146, 263, 489
AcyI GRCGYC 1 cut(s) 23
AfaI GTAC 1 cut(s) 101
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 7 cut(s) 52, 262, 316, 385, 560, 709, 808
AjnI CCWGG 1 cut(s) 168
AleI CACNNNNGTG 1 cut(s) 451
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 2 cut(s) 67, 764
AlwI GGATC 2 cut(s) 392, 405
Aor13HI TCCGGA 1 cut(s) 295
AoxI GGCC 5 cut(s) 105, 129, 150, 171, 377
ApeKI GCWGC 4 cut(s) 111, 140, 530, 581
AseI ATTAAT 1 cut(s) 537
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 4 cut(s) 166, 181, 377, 698
AsuC2I CCSGG 3 cut(s) 46, 104, 185
AsuHPI GGTGA 3 cut(s) 109, 743, 805
AvaII GGWCC 3 cut(s) 166, 181, 698
AxyI CCTNAGG 1 cut(s) 420
BamHI GGATCC 1 cut(s) 397
BanII GRGCYC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 72
BbvI GCAGC 4 cut(s) 123, 152, 542, 568
BccI CCATC 4 cut(s) 182, 327, 606, 709
BceAI ACGGC 3 cut(s) 116, 128, 144
BcgI CGANNNNNNTGC 4 cut(s) 6, 40, 383, 417
BciT130I CCWGG 1 cut(s) 170
BciVI GTATCC 1 cut(s) 316
BclI TGATCA 1 cut(s) 795
BcnI CCSGG 3 cut(s) 46, 104, 185
BcoDI GTCTC 2 cut(s) 67, 764
BfaI CTAG 2 cut(s) 203, 515
BfuI GTATCC 1 cut(s) 316
BglI GCCNNNNNGGC 1 cut(s) 149
BisI GCNGC 4 cut(s) 112, 141, 531, 582
BlsI GCNGC 4 cut(s) 113, 142, 532, 583
Bme1390I CCNGG 4 cut(s) 46, 104, 170, 185
Bme18I GGWCC 3 cut(s) 166, 181, 698
BmgT120I GGNCC 4 cut(s) 166, 181, 377, 698
BmiI GGNNCC 1 cut(s) 399
BmrFI CCNGG 4 cut(s) 46, 104, 170, 185
BmsI GCATC 1 cut(s) 223
BoxI GACNNNNGTC 1 cut(s) 131
BpuMI CCSGG 3 cut(s) 46, 104, 185
Bsa29I ATCGAT 2 cut(s) 231, 610
BsaHI GRCGYC 1 cut(s) 23
BsaI GGTCTC 1 cut(s) 67
BsaJI CCNNGG 3 cut(s) 73, 153, 624
BsaWI WCCGGW 1 cut(s) 295
Bse1I ACTGG 1 cut(s) 727
Bse21I CCTNAGG 1 cut(s) 420
Bse3DI GCAATG 2 cut(s) 236, 651
BseAI TCCGGA 1 cut(s) 295
BseBI CCWGG 1 cut(s) 170
BseCI ATCGAT 2 cut(s) 231, 610
BseDI CCNNGG 3 cut(s) 73, 153, 624
BseGI GGATG 2 cut(s) 309, 701
BseMI GCAATG 2 cut(s) 236, 651
BseMII CTCAG 1 cut(s) 371
BseNI ACTGG 1 cut(s) 727
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 4 cut(s) 123, 152, 542, 568
BsgI GTGCAG 1 cut(s) 387
Bsh1236I CGCG 2 cut(s) 91, 192
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 5 cut(s) 107, 131, 152, 173, 379
BshVI ATCGAT 2 cut(s) 231, 610
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 72
BsiSI CCGG 4 cut(s) 45, 104, 184, 296
BsmAI GTCTC 2 cut(s) 67, 764
BsnI GGCC 5 cut(s) 107, 131, 152, 173, 379
Bso31I GGTCTC 1 cut(s) 67
Bsp1286I GDGCHC 1 cut(s) 72
Bsp13I TCCGGA 1 cut(s) 295
Bsp143I GATC 6 cut(s) 39, 228, 397, 607, 757, 795
Bsp19I CCATGG 1 cut(s) 153
Bsp68I TCGCGA 1 cut(s) 192
BspANI GGCC 5 cut(s) 107, 131, 152, 173, 379
BspCNI CTCAG 1 cut(s) 370
BspDI ATCGAT 2 cut(s) 231, 610
BspEI TCCGGA 1 cut(s) 295
BspFNI CGCG 2 cut(s) 91, 192
BspLI GGNNCC 1 cut(s) 399
BspPI GGATC 2 cut(s) 392, 405
BspQI GCTCTTC 1 cut(s) 465
BspTNI GGTCTC 1 cut(s) 67
BsrDI GCAATG 2 cut(s) 236, 651
BsrI ACTGG 1 cut(s) 727
BssECI CCNNGG 3 cut(s) 73, 153, 624
BssMI GATC 6 cut(s) 39, 228, 397, 607, 757, 795
BssNI GRCGYC 1 cut(s) 23
BssT1I CCWWGG 3 cut(s) 73, 153, 624
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 2 cut(s) 56, 742
Bst6I CTCTTC 1 cut(s) 465
BstACI GRCGYC 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 2 cut(s) 357, 420
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 2 cut(s) 309, 701
BstFNI CGCG 2 cut(s) 91, 192
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 6 cut(s) 42, 231, 400, 610, 760, 798
BstMAI GTCTC 2 cut(s) 67, 764
BstMBI GATC 6 cut(s) 39, 228, 397, 607, 757, 795
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 5 cut(s) 14, 76, 137, 149, 385
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 100
BstPAI GACNNNNGTC 1 cut(s) 131
BstSCI CCNGG 4 cut(s) 44, 102, 168, 183
BstUI CGCG 2 cut(s) 91, 192
BstV1I GCAGC 4 cut(s) 123, 152, 542, 568
BstX2I RGATCY 1 cut(s) 397
BstXI CCANNNNNNTGG 1 cut(s) 453
BstYI RGATCY 1 cut(s) 397
BstZI CGGCCG 1 cut(s) 129
Bsu15I ATCGAT 2 cut(s) 231, 610
Bsu36I CCTNAGG 1 cut(s) 420
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 5 cut(s) 107, 131, 152, 173, 379
BsuTUI ATCGAT 2 cut(s) 231, 610
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 2 cut(s) 309, 701
BtsIMutI CAGTG 2 cut(s) 720, 814
BtuMI TCGCGA 1 cut(s) 192
Cac8I GCNNGC 1 cut(s) 109
CfoI GCGC 1 cut(s) 91
Cfr13I GGNCC 4 cut(s) 166, 181, 377, 698
ClaI ATCGAT 2 cut(s) 231, 610
CseI GACGC 3 cut(s) 12, 103, 146
Csp6I GTAC 1 cut(s) 100
CviAII CATG 7 cut(s) 97, 154, 290, 496, 544, 650, 691
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 2 cut(s) 357, 420
DpnI GATC 6 cut(s) 41, 230, 399, 609, 759, 797
DpnII GATC 6 cut(s) 39, 228, 397, 607, 757, 795
EaeI YGGCCR 2 cut(s) 105, 129
EagI CGGCCG 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 465
EarI CTCTTC 1 cut(s) 465
Ecl136II GAGCTC 1 cut(s) 70
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 3 cut(s) 73, 153, 624
Eco24I GRGCYC 1 cut(s) 72
Eco31I GGTCTC 1 cut(s) 67
Eco47I GGWCC 3 cut(s) 166, 181, 698
Eco52I CGGCCG 1 cut(s) 129
Eco53kI GAGCTC 1 cut(s) 70
Eco57I CTGAAG 3 cut(s) 146, 263, 489
Eco81I CCTNAGG 1 cut(s) 420
EcoICRI GAGCTC 1 cut(s) 70
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 3 cut(s) 73, 153, 624
EcoT22I ATGCAT 2 cut(s) 238, 651
EcoT38I GRGCYC 1 cut(s) 72
ErhI CCWWGG 3 cut(s) 73, 153, 624
FaeI CATG 7 cut(s) 100, 157, 293, 499, 547, 653, 694
FatI CATG 7 cut(s) 96, 153, 289, 495, 543, 649, 690
FauNDI CATATG 1 cut(s) 553
FbaI TGATCA 1 cut(s) 795
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 4 cut(s) 112, 141, 531, 582
FokI GGATG 2 cut(s) 316, 688
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 4 cut(s) 112, 141, 531, 582
FspBI CTAG 2 cut(s) 203, 515
GlaI GCGC 1 cut(s) 90
GluI GCNGC 4 cut(s) 112, 141, 531, 582
HaeIII GGCC 5 cut(s) 107, 131, 152, 173, 379
HapII CCGG 4 cut(s) 45, 104, 184, 296
HgaI GACGC 3 cut(s) 12, 103, 146
HhaI GCGC 1 cut(s) 91
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 7 cut(s) 100, 157, 293, 499, 547, 653, 694
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HindIII AAGCTT 2 cut(s) 386, 710
HinfI GANTC 3 cut(s) 32, 316, 479
HpaII CCGG 4 cut(s) 45, 104, 184, 296
HphI GGTGA 3 cut(s) 109, 743, 805
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 1 cut(s) 67
Hpy188III TCNNGA 5 cut(s) 191, 296, 320, 347, 680
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 4 cut(s) 28, 137, 140, 197
HpyAV CCTTC 5 cut(s) 142, 256, 498, 514, 755
HpyCH4III ACNGT 2 cut(s) 56, 742
HpyCH4V TGCA 4 cut(s) 236, 404, 530, 649
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 76, 137, 149, 385
HpyF3I CTNAG 2 cut(s) 357, 420
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 7 cut(s) 100, 157, 293, 499, 547, 653, 694
HspAI GCGC 1 cut(s) 89
Kpn2I TCCGGA 1 cut(s) 295
Ksp22I TGATCA 1 cut(s) 795
Kzo9I GATC 6 cut(s) 39, 228, 397, 607, 757, 795
LguI GCTCTTC 1 cut(s) 465
LmnI GCTCC 4 cut(s) 67, 75, 157, 589
Lsp1109I GCAGC 4 cut(s) 123, 152, 542, 568
LweI GCATC 1 cut(s) 223
MaeI CTAG 2 cut(s) 203, 515
MaeIII GTNAC 3 cut(s) 115, 547, 749
MalI GATC 6 cut(s) 41, 230, 399, 609, 759, 797
MboI GATC 6 cut(s) 39, 228, 397, 607, 757, 795
MboII GAAGA 1 cut(s) 482
MflI RGATCY 1 cut(s) 397
MhlI GDGCHC 1 cut(s) 72
MluCI AATT 3 cut(s) 257, 565, 777
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 1 cut(s) 9
MnlI CCTC 2 cut(s) 388, 494
Mph1103I ATGCAT 2 cut(s) 238, 651
MroI TCCGGA 1 cut(s) 295
MseI TTAA 1 cut(s) 537
MslI CAYNNNNRTG 1 cut(s) 451
MspI CCGG 4 cut(s) 45, 104, 184, 296
MspR9I CCNGG 4 cut(s) 46, 104, 170, 185
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 2 cut(s) 91, 192
MwoI GCNNNNNNNGC 5 cut(s) 14, 76, 137, 149, 385
NciI CCSGG 3 cut(s) 46, 104, 185
NcoI CCATGG 1 cut(s) 153
NdeI CATATG 1 cut(s) 553
NdeII GATC 6 cut(s) 39, 228, 397, 607, 757, 795
NlaIII CATG 7 cut(s) 100, 157, 293, 499, 547, 653, 694
NlaIV GGNNCC 1 cut(s) 399
NmuCI GTSAC 2 cut(s) 115, 749
NruI TCGCGA 1 cut(s) 192
NsiI ATGCAT 2 cut(s) 238, 651
NspI RCATGY 1 cut(s) 100
OliI CACNNNNGTG 1 cut(s) 451
PciI ACATGT 1 cut(s) 96
PciSI GCTCTTC 1 cut(s) 465
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PfeI GAWTC 2 cut(s) 316, 479
PkrI GCNGC 4 cut(s) 113, 142, 532, 583
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PscI ACATGT 1 cut(s) 96
PshAI GACNNNNGTC 1 cut(s) 131
PshBI ATTAAT 1 cut(s) 537
Psp124BI GAGCTC 1 cut(s) 72
Psp6I CCWGG 1 cut(s) 168
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 1 cut(s) 399
PspPI GGNCC 4 cut(s) 166, 181, 377, 698
PsuI RGATCY 1 cut(s) 397
RruI TCGCGA 1 cut(s) 192
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
RseI CAYNNNNRTG 1 cut(s) 451
SacI GAGCTC 1 cut(s) 72
SalI GTCGAC 1 cut(s) 134
SapI GCTCTTC 1 cut(s) 465
SaqAI TTAA 1 cut(s) 537
SatI GCNGC 4 cut(s) 112, 141, 531, 582
Sau3AI GATC 6 cut(s) 39, 228, 397, 607, 757, 795
Sau96I GGNCC 4 cut(s) 166, 181, 377, 698
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 4 cut(s) 46, 104, 170, 185
SduI GDGCHC 1 cut(s) 72
SfaNI GCATC 1 cut(s) 223
SgrDI CGTCGACG 1 cut(s) 134
SinI GGWCC 3 cut(s) 166, 181, 698
SmiMI CAYNNNNRTG 1 cut(s) 451
Sse9I AATT 3 cut(s) 257, 565, 777
SspMI CTAG 2 cut(s) 203, 515
SstI GAGCTC 1 cut(s) 72
StyD4I CCNGG 4 cut(s) 44, 102, 168, 183
StyI CCWWGG 3 cut(s) 73, 153, 624
TaaI ACNGT 2 cut(s) 56, 742
TaqI TCGA 4 cut(s) 135, 231, 610, 681
TasI AATT 3 cut(s) 257, 565, 777
TfiI GAWTC 2 cut(s) 316, 479
Tru1I TTAA 1 cut(s) 537
Tru9I TTAA 1 cut(s) 537
TscAI CASTG 1 cut(s) 727
TseFI GTSAC 2 cut(s) 115, 749
TseI GCWGC 4 cut(s) 111, 140, 530, 581
Tsp45I GTSAC 2 cut(s) 115, 749
TspDTI ATGAA 2 cut(s) 666, 707
TspRI CASTG 1 cut(s) 727
VpaK11BI GGWCC 3 cut(s) 166, 181, 698
VspI ATTAAT 1 cut(s) 537
XceI RCATGY 1 cut(s) 100
XmiI GTMKAC 1 cut(s) 135
XspI CTAG 2 cut(s) 203, 515
Zsp2I ATGCAT 2 cut(s) 238, 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.