Rw1G018630

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001324
Physical Location & Seq
Forward (+)
41011106 .. 41014322
3217 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G018630.1

Sequence Viewer

Length: 696 bp
ATGGAGGCTGAGAAAGCAGGAGCGTATACCGTCACCGATTTGACGGCCGTCGACGCCGCCGTAAAGGCCATGGAGCTTCAGGTCCAGGCCATCAAGGACCTGGTTCGCGACGAAACCCTCGCCATCCCCAAAGCAAAAAGTAGTTCCCTCTCTCTCTCTCTCAGAAAACTAATCGATGCCTCGCTGAAGCAGCAAAGGAAATTGAAGGACATGTCAATTTATGCGCCCAGTAATGTTCCTGAAAGGATGTCAATGTTGAATTTGGATACAAATAGATGTTTATTTCCAGATAGCTCTCAGCAAAACACTGGTTCTGGGACCTTCAAGCTTGACGAGGATCCTGCACCACCACCAGCACCGCCTAAGGAGAAAAAGGGTCGTTCCACGCCACCATTGTGGTTTATAAGTGCTGAAGAGCTGGATTCCTTGTCACCATACATGAGAGGAAGACTTACACTAGACAAGGTCAATGCAGCTATTAGAGATCGATGGGAAAAAGCCTTGGAAGTAAGAGATATAGCAATGCATGAAGCAGTAAAGGGAAAACACTTCTTTCTCGAAACTGACATAAAGGGACCATCCTTGAAGCTTGACAACTCTGGAAAAGCAATACTAACTGTCCTTCGTCACCTTGGTCGTGTAAGTGAGGCATGGATTGGTCATCATCGGGTGATCATTCTTTTGAACCCTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

231

Amino Acids

25.69

Weight (kDa)

9.08

Isoelectric Point (pI)

42.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 8 - 161 1.6e-28 Spindle and kinetochore-associated protein 1
SKA1 PF07160 162 - 221 8.6e-22 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 404
AccI GTMKAC 2 cut(s) 26, 51
AccII CGCG 1 cut(s) 108
AciI CCGC 2 cut(s) 57, 359
AclWI GGATC 2 cut(s) 332, 345
AcoI YGGCCR 1 cut(s) 45
AcsI RAATTY 1 cut(s) 259
AcuI CTGAAG 3 cut(s) 62, 206, 432
AcyI GRCGYC 1 cut(s) 54
AflIII ACRYGT 1 cut(s) 210
AgsI TTSAA 5 cut(s) 205, 259, 325, 586, 685
AjnI CCWGG 2 cut(s) 84, 99
AleI CACNNNNGTG 1 cut(s) 394
AluBI AGCT 6 cut(s) 76, 294, 328, 418, 476, 589
AluI AGCT 6 cut(s) 76, 294, 328, 418, 476, 589
AlwI GGATC 2 cut(s) 332, 345
AoxI GGCC 3 cut(s) 45, 66, 87
ApeKI GCWGC 2 cut(s) 190, 473
ApoI RAATTY 1 cut(s) 259
AspLEI GCGC 1 cut(s) 226
AspS9I GGNCC 4 cut(s) 82, 97, 318, 575
AsuHPI GGTGA 4 cut(s) 25, 423, 620, 682
AvaII GGWCC 4 cut(s) 82, 97, 318, 575
AxyI CCTNAGG 1 cut(s) 363
BamHI GGATCC 1 cut(s) 337
BbsI GAAGAC 1 cut(s) 454
BbvI GCAGC 2 cut(s) 202, 485
BccI CCATC 4 cut(s) 98, 131, 483, 586
BceAI ACGGC 3 cut(s) 32, 44, 60
BcgI CGANNNNNNTGC 2 cut(s) 323, 357
BciT130I CCWGG 2 cut(s) 86, 101
BciVI GTATCC 1 cut(s) 259
BclI TGATCA 1 cut(s) 672
BfaI CTAG 1 cut(s) 458
BfuI GTATCC 1 cut(s) 259
BglI GCCNNNNNGGC 1 cut(s) 65
BisI GCNGC 3 cut(s) 57, 191, 474
BlsI GCNGC 3 cut(s) 58, 192, 475
Bme1390I CCNGG 2 cut(s) 86, 101
Bme18I GGWCC 4 cut(s) 82, 97, 318, 575
BmgT120I GGNCC 4 cut(s) 82, 97, 318, 575
BmiI GGNNCC 3 cut(s) 319, 339, 576
BmrFI CCNGG 2 cut(s) 86, 101
BmrI ACTGGG 1 cut(s) 222
BmsI GCATC 1 cut(s) 166
BmuI ACTGGG 1 cut(s) 222
BoxI GACNNNNGTC 1 cut(s) 47
BpiI GAAGAC 1 cut(s) 454
Bsa29I ATCGAT 2 cut(s) 174, 487
BsaHI GRCGYC 1 cut(s) 54
BsaJI CCNNGG 3 cut(s) 69, 501, 631
Bse1I ACTGG 2 cut(s) 228, 313
Bse21I CCTNAGG 1 cut(s) 363
Bse3DI GCAATG 1 cut(s) 528
BseBI CCWGG 2 cut(s) 86, 101
BseCI ATCGAT 2 cut(s) 174, 487
BseDI CCNNGG 3 cut(s) 69, 501, 631
BseGI GGATG 3 cut(s) 123, 252, 578
BseMI GCAATG 1 cut(s) 528
BseMII CTCAG 2 cut(s) 175, 311
BseNI ACTGG 2 cut(s) 228, 313
BseX3I CGGCCG 1 cut(s) 45
BseXI GCAGC 2 cut(s) 202, 485
BsgI GTGCAG 1 cut(s) 327
Bsh1236I CGCG 1 cut(s) 108
Bsh1285I CGRYCG 1 cut(s) 48
BshFI GGCC 3 cut(s) 47, 68, 89
BshVI ATCGAT 2 cut(s) 174, 487
BsiEI CGRYCG 1 cut(s) 48
BslFI GGGAC 2 cut(s) 331, 588
BsmFI GGGAC 2 cut(s) 331, 588
BsnI GGCC 3 cut(s) 47, 68, 89
Bsp143I GATC 3 cut(s) 337, 484, 672
Bsp19I CCATGG 1 cut(s) 69
Bsp68I TCGCGA 1 cut(s) 108
BspACI CCGC 2 cut(s) 57, 359
BspANI GGCC 3 cut(s) 47, 68, 89
BspCNI CTCAG 2 cut(s) 174, 310
BspDI ATCGAT 2 cut(s) 174, 487
BspFNI CGCG 1 cut(s) 108
BspLI GGNNCC 3 cut(s) 319, 339, 576
BspPI GGATC 2 cut(s) 332, 345
BspQI GCTCTTC 1 cut(s) 408
BsrDI GCAATG 1 cut(s) 528
BsrI ACTGG 2 cut(s) 228, 313
BssECI CCNNGG 3 cut(s) 69, 501, 631
BssMI GATC 3 cut(s) 337, 484, 672
BssNAI GTATAC 1 cut(s) 27
BssNI GRCGYC 1 cut(s) 54
BssT1I CCWWGG 3 cut(s) 69, 501, 631
Bst1107I GTATAC 1 cut(s) 27
Bst2UI CCWGG 2 cut(s) 86, 101
Bst4CI ACNGT 2 cut(s) 31, 619
Bst6I CTCTTC 1 cut(s) 408
BstACI GRCGYC 1 cut(s) 54
BstDEI CTNAG 4 cut(s) 9, 161, 297, 363
BstDSI CCRYGG 1 cut(s) 69
BstF5I GGATG 3 cut(s) 123, 252, 578
BstFNI CGCG 1 cut(s) 108
BstHHI GCGC 1 cut(s) 226
BstKTI GATC 3 cut(s) 340, 487, 675
BstMBI GATC 3 cut(s) 337, 484, 672
BstMCI CGRYCG 1 cut(s) 48
BstMWI GCNNNNNNNGC 4 cut(s) 14, 53, 65, 190
BstNI CCWGG 2 cut(s) 86, 101
BstNSI RCATGY 1 cut(s) 214
BstPAI GACNNNNGTC 1 cut(s) 47
BstSCI CCNGG 2 cut(s) 84, 99
BstUI CGCG 1 cut(s) 108
BstV1I GCAGC 2 cut(s) 202, 485
BstV2I GAAGAC 1 cut(s) 454
BstX2I RGATCY 1 cut(s) 337
BstXI CCANNNNNNTGG 1 cut(s) 396
BstYI RGATCY 1 cut(s) 337
BstZ17I GTATAC 1 cut(s) 27
BstZI CGGCCG 1 cut(s) 45
Bsu15I ATCGAT 2 cut(s) 174, 487
Bsu36I CCTNAGG 1 cut(s) 363
BsuI GTATCC 1 cut(s) 259
BsuRI GGCC 3 cut(s) 47, 68, 89
BsuTUI ATCGAT 2 cut(s) 174, 487
BtgI CCRYGG 1 cut(s) 69
BtsCI GGATG 3 cut(s) 123, 252, 578
BtsIMutI CAGTG 2 cut(s) 306, 691
BtuMI TCGCGA 1 cut(s) 108
CfoI GCGC 1 cut(s) 226
Cfr13I GGNCC 4 cut(s) 82, 97, 318, 575
ClaI ATCGAT 2 cut(s) 174, 487
CseI GACGC 1 cut(s) 62
CsiI ACCWGGT 1 cut(s) 99
CviAII CATG 5 cut(s) 70, 211, 439, 527, 651
DdeI CTNAG 4 cut(s) 9, 161, 297, 363
DpnI GATC 3 cut(s) 339, 486, 674
DpnII GATC 3 cut(s) 337, 484, 672
EaeI YGGCCR 1 cut(s) 45
EagI CGGCCG 1 cut(s) 45
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
EclXI CGGCCG 1 cut(s) 45
Eco130I CCWWGG 3 cut(s) 69, 501, 631
Eco47I GGWCC 4 cut(s) 82, 97, 318, 575
Eco52I CGGCCG 1 cut(s) 45
Eco57I CTGAAG 3 cut(s) 62, 206, 432
Eco81I CCTNAGG 1 cut(s) 363
EcoO109I RGGNCCY 2 cut(s) 97, 318
EcoRII CCWGG 2 cut(s) 84, 99
EcoT14I CCWWGG 3 cut(s) 69, 501, 631
EcoT22I ATGCAT 1 cut(s) 528
ErhI CCWWGG 3 cut(s) 69, 501, 631
FaeI CATG 5 cut(s) 73, 214, 442, 530, 654
FaqI GGGAC 2 cut(s) 331, 588
FatI CATG 5 cut(s) 69, 210, 438, 526, 650
FbaI TGATCA 1 cut(s) 672
FblI GTMKAC 2 cut(s) 26, 51
Fnu4HI GCNGC 3 cut(s) 57, 191, 474
FokI GGATG 3 cut(s) 110, 259, 565
Fsp4HI GCNGC 3 cut(s) 57, 191, 474
FspBI CTAG 1 cut(s) 458
GlaI GCGC 1 cut(s) 225
GluI GCNGC 3 cut(s) 57, 191, 474
HaeIII GGCC 3 cut(s) 47, 68, 89
HgaI GACGC 1 cut(s) 62
HhaI GCGC 1 cut(s) 226
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 5 cut(s) 73, 214, 442, 530, 654
Hin6I GCGC 1 cut(s) 224
HinP1I GCGC 1 cut(s) 224
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HindIII AAGCTT 2 cut(s) 326, 587
HinfI GANTC 1 cut(s) 422
HphI GGTGA 4 cut(s) 25, 423, 620, 682
Hpy166II GTNNAC 2 cut(s) 27, 52
Hpy188I TCNGA 1 cut(s) 164
Hpy188III TCNNGA 5 cut(s) 107, 239, 287, 557, 600
Hpy8I GTNNAC 2 cut(s) 27, 52
Hpy99I CGWCG 3 cut(s) 53, 56, 113
HpyAV CCTTC 3 cut(s) 199, 331, 632
HpyCH4III ACNGT 2 cut(s) 31, 619
HpyCH4V TGCA 3 cut(s) 344, 473, 526
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 53, 65, 190
HpyF3I CTNAG 4 cut(s) 9, 161, 297, 363
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 5 cut(s) 73, 214, 442, 530, 654
HspAI GCGC 1 cut(s) 224
Ksp22I TGATCA 1 cut(s) 672
Kzo9I GATC 3 cut(s) 337, 484, 672
LguI GCTCTTC 1 cut(s) 408
LmnI GCTCC 2 cut(s) 20, 73
Lsp1109I GCAGC 2 cut(s) 202, 485
LweI GCATC 1 cut(s) 166
MabI ACCWGGT 1 cut(s) 99
MaeI CTAG 1 cut(s) 458
MaeIII GTNAC 3 cut(s) 31, 429, 626
MalI GATC 3 cut(s) 339, 486, 674
MboI GATC 3 cut(s) 337, 484, 672
MboII GAAGA 2 cut(s) 425, 459
MflI RGATCY 1 cut(s) 337
MluCI AATT 3 cut(s) 200, 216, 259
MnlI CCTC 6 cut(s) 128, 158, 190, 328, 437, 640
Mph1103I ATGCAT 1 cut(s) 528
MslI CAYNNNNRTG 1 cut(s) 394
MspR9I CCNGG 2 cut(s) 86, 101
MvaI CCWGG 2 cut(s) 86, 101
MvnI CGCG 1 cut(s) 108
MwoI GCNNNNNNNGC 4 cut(s) 14, 53, 65, 190
NcoI CCATGG 1 cut(s) 69
NdeII GATC 3 cut(s) 337, 484, 672
NlaIII CATG 5 cut(s) 73, 214, 442, 530, 654
NlaIV GGNNCC 3 cut(s) 319, 339, 576
NmuCI GTSAC 3 cut(s) 31, 429, 626
NruI TCGCGA 1 cut(s) 108
NsiI ATGCAT 1 cut(s) 528
NspI RCATGY 1 cut(s) 214
OliI CACNNNNGTG 1 cut(s) 394
PciI ACATGT 1 cut(s) 210
PciSI GCTCTTC 1 cut(s) 408
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PfeI GAWTC 1 cut(s) 422
PflFI GACNNNGTC 1 cut(s) 464
PkrI GCNGC 3 cut(s) 58, 192, 475
PpuMI RGGWCCY 2 cut(s) 97, 318
PscI ACATGT 1 cut(s) 210
PshAI GACNNNNGTC 1 cut(s) 47
PsiI TTATAA 1 cut(s) 404
Psp5II RGGWCCY 2 cut(s) 97, 318
Psp6I CCWGG 2 cut(s) 84, 99
PspGI CCWGG 2 cut(s) 84, 99
PspN4I GGNNCC 3 cut(s) 319, 339, 576
PspPI GGNCC 4 cut(s) 82, 97, 318, 575
PspPPI RGGWCCY 2 cut(s) 97, 318
PsuI RGATCY 1 cut(s) 337
PsyI GACNNNGTC 1 cut(s) 464
RruI TCGCGA 1 cut(s) 108
RseI CAYNNNNRTG 1 cut(s) 394
SalI GTCGAC 1 cut(s) 50
SapI GCTCTTC 1 cut(s) 408
SatI GCNGC 3 cut(s) 57, 191, 474
Sau3AI GATC 3 cut(s) 337, 484, 672
Sau96I GGNCC 4 cut(s) 82, 97, 318, 575
ScrFI CCNGG 2 cut(s) 86, 101
SexAI ACCWGGT 1 cut(s) 99
SfaNI GCATC 1 cut(s) 166
SgrDI CGTCGACG 1 cut(s) 50
SinI GGWCC 4 cut(s) 82, 97, 318, 575
SmiMI CAYNNNNRTG 1 cut(s) 394
Sse9I AATT 3 cut(s) 200, 216, 259
SsiI CCGC 2 cut(s) 57, 359
SspMI CTAG 1 cut(s) 458
StyD4I CCNGG 2 cut(s) 84, 99
StyI CCWWGG 3 cut(s) 69, 501, 631
TaaI ACNGT 2 cut(s) 31, 619
TaqI TCGA 4 cut(s) 51, 174, 487, 558
TasI AATT 3 cut(s) 200, 216, 259
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 1 cut(s) 422
TscAI CASTG 1 cut(s) 313
TseFI GTSAC 3 cut(s) 31, 429, 626
TseI GCWGC 2 cut(s) 190, 473
Tsp45I GTSAC 3 cut(s) 31, 429, 626
TspDTI ATGAA 1 cut(s) 543
TspRI CASTG 1 cut(s) 313
Tth111I GACNNNGTC 1 cut(s) 464
VpaK11BI GGWCC 4 cut(s) 82, 97, 318, 575
XapI RAATTY 1 cut(s) 259
XceI RCATGY 1 cut(s) 214
XcmI CCANNNNNNNNNTGG 1 cut(s) 97
XmiI GTMKAC 2 cut(s) 26, 51
XspI CTAG 1 cut(s) 458
Zsp2I ATGCAT 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.