pycom02g17150

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001324
Physical Location & Seq
Reverse (-)
14743155 .. 14747697
4543 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g17150.4

Sequence Viewer

Length: 873 bp
ATGAGATTTTCCGATCCAACAGAGAGATTGAAAGTTTCTGAAGAAAGGTCGAGAGATAACGAGATGGAGGTAAAGACAGCAGGGGCGTCGTTGGATTCGGTTATCTCAGGGTTCAACCGTCGGATCGCGGAGCTTCAAGATCTCGTCATTGCGCGAAACGTGTACCCGGCGAGCAGCGTCACCGATTTGTCGGCCGTCGACGCGGCCGTGAAGGCCATGGAGCTTCAGGTTCAGGCCATCAAGCACCGCTTGCGTGATGAAACCCTAGCCATCCCTAAAGCTAAAAAACTCGTCGATGCCGCGCTGAAGCAGCAAAAGAAATTGCAGGACATGTCAGTTTATGTGCCCTCGCATGTTCCCGAAAGAATGTCGATGTTGAATTTGGATACTAATAAGTGTTTGTTTCCAGAAGGAACTCAACAAAATACTGGTTTTGGGTCCTTCAAGCTTGAGGAAGAGCCTGCTGCTCTACCTAAGGAGAAAAAGGGACGTTCCCCTCCACCATTGTGGTTTATAACTGCTGAAGAGCTGGATTCTTTGTCAGGGTACATGAGGGGAAGACTTACAGTAGAGAAGATCAATGCAGCTATTAATGACATGGTGACATATGCAGAAGCAAACGCTCAGCTGATATTTGCCCCAAAGAAGAAGGTAGCAGGAGATCTTTGGGAAAAGGCACTGGAAATGAGGGACATTGCAATGACAGAAGGAATAAGGGGAAAACACTTCTTTCTTGAAACTGACATGAAAGGACCAACCTTGAAGCCTGACAACACTGGAAAAGCAATACTAACTGTCCTTCGTCACCTTGGTCGTGTAAGCGAGACGCGTATCGGGCCTCATCGAGTGATCATTCTATTGAAGCGTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

291

Amino Acids

32.44

Weight (kDa)

9.25

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 515
AccI GTMKAC 1 cut(s) 198
AccII CGCG 5 cut(s) 128, 154, 203, 302, 829
AciI CCGC 4 cut(s) 128, 203, 247, 300
AclWI GGATC 2 cut(s) 8, 131
AcoI YGGCCR 2 cut(s) 192, 204
AcsI RAATTY 1 cut(s) 379
AcuI CTGAAG 4 cut(s) 60, 209, 326, 543
AcyI GRCGYC 1 cut(s) 86
AfaI GTAC 2 cut(s) 164, 548
AflIII ACRYGT 3 cut(s) 159, 330, 827
AgsI TTSAA 8 cut(s) 31, 115, 137, 379, 445, 737, 763, 862
AleI CACNNNNGTG 1 cut(s) 505
AluBI AGCT 7 cut(s) 133, 223, 281, 448, 529, 587, 628
AluI AGCT 7 cut(s) 133, 223, 281, 448, 529, 587, 628
Alw26I GTCTC 1 cut(s) 818
AlwI GGATC 2 cut(s) 8, 131
AoxI GGCC 5 cut(s) 192, 204, 213, 234, 836
ApeKI GCWGC 4 cut(s) 174, 310, 464, 584
ApoI RAATTY 1 cut(s) 379
AseI ATTAAT 1 cut(s) 591
AspLEI GCGC 2 cut(s) 154, 304
AspS9I GGNCC 3 cut(s) 438, 752, 836
AsuC2I CCSGG 1 cut(s) 167
AsuHPI GGTGA 3 cut(s) 172, 613, 797
AvaII GGWCC 2 cut(s) 438, 752
AxyI CCTNAGG 1 cut(s) 474
BaeGI GKGCMC 1 cut(s) 348
BbsI GAAGAC 1 cut(s) 565
BbvI GCAGC 4 cut(s) 186, 322, 451, 596
BccI CCATC 3 cut(s) 58, 245, 278
BceAI ACGGC 2 cut(s) 179, 191
BcgI CGANNNNNNTGC 2 cut(s) 69, 103
BciVI GTATCC 1 cut(s) 379
BclI TGATCA 1 cut(s) 849
BcnI CCSGG 1 cut(s) 167
BcoDI GTCTC 1 cut(s) 818
BfaI CTAG 1 cut(s) 266
BfuI GTATCC 1 cut(s) 379
BglI GCCNNNNNGGC 1 cut(s) 212
BglII AGATCT 2 cut(s) 139, 661
BisI GCNGC 6 cut(s) 175, 204, 300, 311, 465, 585
BlpI GCTNAGC 1 cut(s) 624
BlsI GCNGC 6 cut(s) 176, 205, 301, 312, 466, 586
Bme1390I CCNGG 1 cut(s) 167
Bme18I GGWCC 2 cut(s) 438, 752
BmgT120I GGNCC 3 cut(s) 438, 752, 836
BmiI GGNNCC 1 cut(s) 439
BmrFI CCNGG 1 cut(s) 167
BmsI GCATC 1 cut(s) 286
BpiI GAAGAC 1 cut(s) 565
Bpu1102I GCTNAGC 1 cut(s) 624
BpuEI CTTGAG 1 cut(s) 470
BpuMI CCSGG 1 cut(s) 167
BsaHI GRCGYC 1 cut(s) 86
BsaJI CCNNGG 2 cut(s) 216, 808
Bse1I ACTGG 3 cut(s) 433, 684, 781
Bse21I CCTNAGG 1 cut(s) 474
Bse3DI GCAATG 3 cut(s) 147, 693, 705
BseDI CCNNGG 2 cut(s) 216, 808
BseGI GGATG 1 cut(s) 270
BseMI GCAATG 3 cut(s) 147, 693, 705
BseMII CTCAG 2 cut(s) 120, 638
BseNI ACTGG 3 cut(s) 433, 684, 781
BseSI GKGCMC 1 cut(s) 348
BseX3I CGGCCG 2 cut(s) 192, 204
BseXI GCAGC 4 cut(s) 186, 322, 451, 596
Bsh1236I CGCG 5 cut(s) 128, 154, 203, 302, 829
Bsh1285I CGRYCG 2 cut(s) 195, 207
BshFI GGCC 5 cut(s) 194, 206, 215, 236, 838
BsiEI CGRYCG 2 cut(s) 195, 207
BsiSI CCGG 1 cut(s) 167
BslFI GGGAC 2 cut(s) 501, 704
BsmAI GTCTC 1 cut(s) 818
BsmBI CGTCTC 1 cut(s) 818
BsmFI GGGAC 2 cut(s) 501, 704
BsnI GGCC 5 cut(s) 194, 206, 215, 236, 838
Bsp1286I GDGCHC 1 cut(s) 348
Bsp143I GATC 6 cut(s) 13, 123, 139, 576, 661, 849
Bsp1720I GCTNAGC 1 cut(s) 624
Bsp19I CCATGG 1 cut(s) 216
BspACI CCGC 4 cut(s) 128, 203, 247, 300
BspANI GGCC 5 cut(s) 194, 206, 215, 236, 838
BspCNI CTCAG 2 cut(s) 119, 637
BspFNI CGCG 5 cut(s) 128, 154, 203, 302, 829
BspLI GGNNCC 1 cut(s) 439
BspPI GGATC 2 cut(s) 8, 131
BspQI GCTCTTC 2 cut(s) 450, 519
BsrDI GCAATG 3 cut(s) 147, 693, 705
BsrI ACTGG 3 cut(s) 433, 684, 781
BssECI CCNNGG 2 cut(s) 216, 808
BssMI GATC 6 cut(s) 13, 123, 139, 576, 661, 849
BssNI GRCGYC 1 cut(s) 86
BssT1I CCWWGG 2 cut(s) 216, 808
Bst4CI ACNGT 3 cut(s) 119, 568, 796
Bst6I CTCTTC 2 cut(s) 450, 519
BstACI GRCGYC 1 cut(s) 86
BstAPI GCANNNNNTGC 1 cut(s) 250
BstC8I GCNNGC 3 cut(s) 172, 251, 462
BstDEI CTNAG 3 cut(s) 106, 474, 624
BstDSI CCRYGG 1 cut(s) 216
BstF5I GGATG 1 cut(s) 270
BstFNI CGCG 5 cut(s) 128, 154, 203, 302, 829
BstHHI GCGC 2 cut(s) 154, 304
BstKTI GATC 6 cut(s) 16, 126, 142, 579, 664, 852
BstMAI GTCTC 1 cut(s) 818
BstMBI GATC 6 cut(s) 13, 123, 139, 576, 661, 849
BstMCI CGRYCG 2 cut(s) 195, 207
BstMWI GCNNNNNNNGC 5 cut(s) 200, 212, 250, 310, 835
BstNSI RCATGY 2 cut(s) 334, 356
BstSCI CCNGG 1 cut(s) 165
BstSLI GKGCMC 1 cut(s) 348
BstUI CGCG 5 cut(s) 128, 154, 203, 302, 829
BstV1I GCAGC 4 cut(s) 186, 322, 451, 596
BstV2I GAAGAC 1 cut(s) 565
BstX2I RGATCY 2 cut(s) 139, 661
BstXI CCANNNNNNTGG 1 cut(s) 507
BstYI RGATCY 2 cut(s) 139, 661
BstZI CGGCCG 2 cut(s) 192, 204
Bsu36I CCTNAGG 1 cut(s) 474
BsuI GTATCC 1 cut(s) 379
BsuRI GGCC 5 cut(s) 194, 206, 215, 236, 838
BtgI CCRYGG 1 cut(s) 216
BtsCI GGATG 1 cut(s) 270
BtsIMutI CAGTG 2 cut(s) 677, 774
Cac8I GCNNGC 3 cut(s) 172, 251, 462
CfoI GCGC 2 cut(s) 154, 304
Cfr13I GGNCC 3 cut(s) 438, 752, 836
CseI GACGC 5 cut(s) 75, 166, 209, 835, 854
Csp6I GTAC 2 cut(s) 163, 547
CviAII CATG 6 cut(s) 217, 331, 353, 550, 598, 745
CviQI GTAC 2 cut(s) 163, 547
DdeI CTNAG 3 cut(s) 106, 474, 624
DpnI GATC 6 cut(s) 15, 125, 141, 578, 663, 851
DpnII GATC 6 cut(s) 13, 123, 139, 576, 661, 849
EaeI YGGCCR 2 cut(s) 192, 204
EagI CGGCCG 2 cut(s) 192, 204
Eam1104I CTCTTC 2 cut(s) 450, 519
EarI CTCTTC 2 cut(s) 450, 519
EclXI CGGCCG 2 cut(s) 192, 204
Eco130I CCWWGG 2 cut(s) 216, 808
Eco47I GGWCC 2 cut(s) 438, 752
Eco52I CGGCCG 2 cut(s) 192, 204
Eco57I CTGAAG 4 cut(s) 60, 209, 326, 543
Eco81I CCTNAGG 1 cut(s) 474
EcoO109I RGGNCCY 1 cut(s) 438
EcoT14I CCWWGG 2 cut(s) 216, 808
ErhI CCWWGG 2 cut(s) 216, 808
Esp3I CGTCTC 1 cut(s) 818
FaeI CATG 6 cut(s) 220, 334, 356, 553, 601, 748
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FaqI GGGAC 2 cut(s) 501, 704
FatI CATG 6 cut(s) 216, 330, 352, 549, 597, 744
FauNDI CATATG 1 cut(s) 607
FbaI TGATCA 1 cut(s) 849
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 6 cut(s) 175, 204, 300, 311, 465, 585
FokI GGATG 1 cut(s) 257
Fsp4HI GCNGC 6 cut(s) 175, 204, 300, 311, 465, 585
FspBI CTAG 1 cut(s) 266
GlaI GCGC 2 cut(s) 153, 303
GluI GCNGC 6 cut(s) 175, 204, 300, 311, 465, 585
HaeIII GGCC 5 cut(s) 194, 206, 215, 236, 838
HapII CCGG 1 cut(s) 167
HgaI GACGC 5 cut(s) 75, 166, 209, 835, 854
HhaI GCGC 2 cut(s) 154, 304
Hin1I GRCGYC 1 cut(s) 86
Hin1II CATG 6 cut(s) 220, 334, 356, 553, 601, 748
Hin6I GCGC 2 cut(s) 152, 302
HinP1I GCGC 2 cut(s) 152, 302
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 2 cut(s) 95, 533
HpaII CCGG 1 cut(s) 167
HphI GGTGA 3 cut(s) 172, 613, 797
Hpy166II GTNNAC 2 cut(s) 163, 199
Hpy188I TCNGA 3 cut(s) 13, 40, 123
Hpy188III TCNNGA 5 cut(s) 51, 137, 359, 407, 734
Hpy8I GTNNAC 2 cut(s) 163, 199
Hpy99I CGWCG 5 cut(s) 91, 123, 200, 203, 296
HpyAV CCTTC 6 cut(s) 205, 404, 451, 643, 701, 809
HpyCH4III ACNGT 3 cut(s) 119, 568, 796
HpyCH4IV ACGT 2 cut(s) 159, 490
HpyCH4V TGCA 4 cut(s) 325, 584, 611, 698
HpyF10VI GCNNNNNNNGC 5 cut(s) 200, 212, 250, 310, 835
HpyF3I CTNAG 3 cut(s) 106, 474, 624
HpySE526I ACGT 2 cut(s) 159, 490
Hsp92I GRCGYC 1 cut(s) 86
Hsp92II CATG 6 cut(s) 220, 334, 356, 553, 601, 748
HspAI GCGC 2 cut(s) 152, 302
Ksp22I TGATCA 1 cut(s) 849
Kzo9I GATC 6 cut(s) 13, 123, 139, 576, 661, 849
LguI GCTCTTC 2 cut(s) 450, 519
LmnI GCTCC 2 cut(s) 130, 220
Lsp1109I GCAGC 4 cut(s) 186, 322, 451, 596
LweI GCATC 1 cut(s) 286
MaeI CTAG 1 cut(s) 266
MaeII ACGT 2 cut(s) 159, 490
MaeIII GTNAC 3 cut(s) 178, 601, 803
MalI GATC 6 cut(s) 15, 125, 141, 578, 663, 851
MboI GATC 6 cut(s) 13, 123, 139, 576, 661, 849
MboII GAAGA 6 cut(s) 53, 467, 536, 570, 586, 658
MflI RGATCY 2 cut(s) 139, 661
MhlI GDGCHC 1 cut(s) 348
MluCI AATT 2 cut(s) 320, 379
MluI ACGCGT 1 cut(s) 827
MmeI TCCRAC 3 cut(s) 41, 72, 101
MnlI CCTC 7 cut(s) 61, 358, 445, 507, 546, 681, 849
MseI TTAA 1 cut(s) 591
MslI CAYNNNNRTG 2 cut(s) 505, 698
MspA1I CMGCKG 1 cut(s) 628
MspI CCGG 1 cut(s) 167
MspR9I CCNGG 1 cut(s) 167
MvnI CGCG 5 cut(s) 128, 154, 203, 302, 829
MwoI GCNNNNNNNGC 5 cut(s) 200, 212, 250, 310, 835
NciI CCSGG 1 cut(s) 167
NcoI CCATGG 1 cut(s) 216
NdeI CATATG 1 cut(s) 607
NdeII GATC 6 cut(s) 13, 123, 139, 576, 661, 849
NlaIII CATG 6 cut(s) 220, 334, 356, 553, 601, 748
NlaIV GGNNCC 1 cut(s) 439
NmuCI GTSAC 3 cut(s) 178, 601, 803
NspI RCATGY 2 cut(s) 334, 356
OliI CACNNNNGTG 1 cut(s) 505
PciI ACATGT 1 cut(s) 330
PciSI GCTCTTC 2 cut(s) 450, 519
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PfeI GAWTC 2 cut(s) 95, 533
PkrI GCNGC 6 cut(s) 176, 205, 301, 312, 466, 586
PpuMI RGGWCCY 1 cut(s) 438
PscI ACATGT 1 cut(s) 330
PshBI ATTAAT 1 cut(s) 591
PsiI TTATAA 1 cut(s) 515
Psp5II RGGWCCY 1 cut(s) 438
PspN4I GGNNCC 1 cut(s) 439
PspPI GGNCC 3 cut(s) 438, 752, 836
PspPPI RGGWCCY 1 cut(s) 438
PsuI RGATCY 2 cut(s) 139, 661
PvuII CAGCTG 1 cut(s) 628
RsaI GTAC 2 cut(s) 164, 548
RsaNI GTAC 2 cut(s) 163, 547
RseI CAYNNNNRTG 2 cut(s) 505, 698
SalI GTCGAC 1 cut(s) 197
SapI GCTCTTC 2 cut(s) 450, 519
SaqAI TTAA 1 cut(s) 591
SatI GCNGC 6 cut(s) 175, 204, 300, 311, 465, 585
Sau3AI GATC 6 cut(s) 13, 123, 139, 576, 661, 849
Sau96I GGNCC 3 cut(s) 438, 752, 836
ScrFI CCNGG 1 cut(s) 167
SduI GDGCHC 1 cut(s) 348
SfaNI GCATC 1 cut(s) 286
SfiI GGCCNNNNNGGCC 1 cut(s) 212
SgrDI CGTCGACG 1 cut(s) 197
SinI GGWCC 2 cut(s) 438, 752
SmiMI CAYNNNNRTG 2 cut(s) 505, 698
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 2 cut(s) 320, 379
SsiI CCGC 4 cut(s) 128, 203, 247, 300
SspMI CTAG 1 cut(s) 266
StyD4I CCNGG 1 cut(s) 165
StyI CCWWGG 2 cut(s) 216, 808
TaaI ACNGT 3 cut(s) 119, 568, 796
TaiI ACGT 2 cut(s) 162, 493
TaqI TCGA 5 cut(s) 50, 198, 294, 371, 844
TasI AATT 2 cut(s) 320, 379
TauI GCSGC 2 cut(s) 206, 302
TfiI GAWTC 2 cut(s) 95, 533
Tru1I TTAA 1 cut(s) 591
Tru9I TTAA 1 cut(s) 591
TscAI CASTG 2 cut(s) 684, 781
TseFI GTSAC 3 cut(s) 178, 601, 803
TseI GCWGC 4 cut(s) 174, 310, 464, 584
Tsp45I GTSAC 3 cut(s) 178, 601, 803
TspDTI ATGAA 2 cut(s) 273, 761
TspRI CASTG 2 cut(s) 684, 781
VpaK11BI GGWCC 2 cut(s) 438, 752
VspI ATTAAT 1 cut(s) 591
XapI RAATTY 1 cut(s) 379
XceI RCATGY 2 cut(s) 334, 356
XmiI GTMKAC 1 cut(s) 198
XspI CTAG 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.