Rw1G018600

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001324
Physical Location & Seq
Forward (+)
40952602 .. 40955894
3293 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G018600.1

Sequence Viewer

Length: 816 bp
ATGGAGGTGGAGAAAGCAAGGGCGTCGTTGGACTCGCTGATCGCCGGGTTCAACAGTCGGATCTCGGAGCTCCAAGACAGCGTCACCGATTTGACTTCCGTCGACGCCGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGCCATCAAGGACCGGGTTCGCGACGAAACCCTAGCCATCCCCAAAGCAAAACTGGTACAATGTGATGCATCGCTGAAGCAGCGAAGGAAATTGAAGGATATGTCATTTTATGCCCCCACTCATGTTCCGGAAAGCATGTCAATGTTGAATTTGGATACAAACAGATGTTTATTTCCAGAAAGCTCTCAGCAAAACAATGGTTTTGGGACCTTCAAGCTTGAGAAGGATCCTGCACCGCCGCCTAAAGAGAAAAAGGGTCGTTCCACACCCCCATTGTGGTTTAGAAGTGCTGAAGAGCTGGAATGCTGGATTCCTTGTCATCGTGATATTACTAACTGCATATACATGAGAGGAAGGCTTACACTAGAGAAGGTCAACACAGCTATTAATGACATGGCAACATATGCTGAAGCAAATTATCAGCTCATAGCTGCTCCAAAGAAGAATGTGGCAGGAGATCGATGGGAAAAAGCCTCGGAAATAAGAGATATAGTAATGCATGAAGCAGTAAAGGGAAAACACTTCTTTCTCGAAACTGACATGAAGGGACCATCCTTGAAGCTTGACAACAGTGGAAAAGCAATACTAACAGTCCTTCGTCACCTTGGTCGTTTAAGTGAGACACGGATTGGTCATAATCGGGTGATCATTCTTTTGCCTCCAAAGAGGAGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

271

Amino Acids

30.66

Weight (kDa)

9.3

Isoelectric Point (pI)

52.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 9 - 258 2e-65 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 102
AccII CGCG 1 cut(s) 159
AccIII TCCGGA 1 cut(s) 265
AciI CCGC 3 cut(s) 108, 374, 377
AclWI GGATC 3 cut(s) 68, 359, 372
AcsI RAATTY 1 cut(s) 286
AcuI CTGAAG 4 cut(s) 113, 233, 450, 567
AcyI GRCGYC 2 cut(s) 23, 105
AfaI GTAC 1 cut(s) 195
AfiI CCNNNNNNNGG 1 cut(s) 414
AgsI TTSAA 5 cut(s) 52, 232, 286, 352, 697
AjnI CCWGG 1 cut(s) 135
AluBI AGCT 9 cut(s) 70, 127, 321, 355, 436, 521, 562, 569, 700
AluI AGCT 9 cut(s) 70, 127, 321, 355, 436, 521, 562, 569, 700
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 1 cut(s) 752
AlwI GGATC 3 cut(s) 68, 359, 372
Aor13HI TCCGGA 1 cut(s) 265
AoxI GGCC 2 cut(s) 117, 138
ApeKI GCWGC 2 cut(s) 217, 569
ApoI RAATTY 1 cut(s) 286
AseI ATTAAT 1 cut(s) 525
AspS9I GGNCC 4 cut(s) 133, 148, 345, 686
AsuC2I CCSGG 2 cut(s) 46, 152
AsuHPI GGTGA 3 cut(s) 76, 731, 793
AvaII GGWCC 4 cut(s) 133, 148, 345, 686
BamHI GGATCC 1 cut(s) 364
BanII GRGCYC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 72
BbvI GCAGC 2 cut(s) 229, 556
BccI CCATC 4 cut(s) 149, 182, 594, 697
BceAI ACGGC 1 cut(s) 95
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BciT130I CCWGG 1 cut(s) 137
BciVI GTATCC 1 cut(s) 286
BclI TGATCA 1 cut(s) 783
BcnI CCSGG 2 cut(s) 46, 152
BcoDI GTCTC 1 cut(s) 752
BfaI CTAG 2 cut(s) 170, 503
BfuI GTATCC 1 cut(s) 286
BglI GCCNNNNNGGC 1 cut(s) 116
BisI GCNGC 4 cut(s) 108, 218, 377, 570
BlsI GCNGC 4 cut(s) 109, 219, 378, 571
Bme1390I CCNGG 3 cut(s) 46, 137, 152
Bme18I GGWCC 4 cut(s) 133, 148, 345, 686
BmgT120I GGNCC 4 cut(s) 133, 148, 345, 686
BmiI GGNNCC 3 cut(s) 346, 366, 687
BmrFI CCNGG 3 cut(s) 46, 137, 152
BmsI GCATC 2 cut(s) 193, 215
BoxI GACNNNNGTC 1 cut(s) 98
BpuEI CTTGAG 1 cut(s) 377
BpuMI CCSGG 2 cut(s) 46, 152
Bsa29I ATCGAT 1 cut(s) 598
BsaHI GRCGYC 2 cut(s) 23, 105
BsaJI CCNNGG 3 cut(s) 120, 612, 742
BsaWI WCCGGW 1 cut(s) 265
Bsc4I CCNNNNNNNGG 1 cut(s) 414
Bse1I ACTGG 1 cut(s) 195
BseAI TCCGGA 1 cut(s) 265
BseBI CCWGG 1 cut(s) 137
BseCI ATCGAT 1 cut(s) 598
BseDI CCNNGG 3 cut(s) 120, 612, 742
BseGI GGATG 2 cut(s) 174, 689
BseLI CCNNNNNNNGG 1 cut(s) 414
BseMII CTCAG 1 cut(s) 338
BseNI ACTGG 1 cut(s) 195
BseXI GCAGC 2 cut(s) 229, 556
BsgI GTGCAG 1 cut(s) 354
Bsh1236I CGCG 1 cut(s) 159
BshFI GGCC 2 cut(s) 119, 140
BshVI ATCGAT 1 cut(s) 598
BsiHKAI GWGCWC 1 cut(s) 72
BsiSI CCGG 3 cut(s) 45, 151, 266
BslFI GGGAC 2 cut(s) 358, 699
BslI CCNNNNNNNGG 1 cut(s) 414
BsmAI GTCTC 1 cut(s) 752
BsmFI GGGAC 2 cut(s) 358, 699
BsmI GAATGC 1 cut(s) 446
BsnI GGCC 2 cut(s) 119, 140
Bsp1286I GDGCHC 1 cut(s) 72
Bsp13I TCCGGA 1 cut(s) 265
Bsp143I GATC 5 cut(s) 39, 60, 364, 595, 783
Bsp19I CCATGG 1 cut(s) 120
Bsp68I TCGCGA 1 cut(s) 159
BspACI CCGC 3 cut(s) 108, 374, 377
BspANI GGCC 2 cut(s) 119, 140
BspCNI CTCAG 1 cut(s) 337
BspDI ATCGAT 1 cut(s) 598
BspEI TCCGGA 1 cut(s) 265
BspFNI CGCG 1 cut(s) 159
BspLI GGNNCC 3 cut(s) 346, 366, 687
BspPI GGATC 3 cut(s) 68, 359, 372
BspQI GCTCTTC 1 cut(s) 426
BsrI ACTGG 1 cut(s) 195
BssECI CCNNGG 3 cut(s) 120, 612, 742
BssMI GATC 5 cut(s) 39, 60, 364, 595, 783
BssNI GRCGYC 2 cut(s) 23, 105
BssT1I CCWWGG 2 cut(s) 120, 742
Bst2UI CCWGG 1 cut(s) 137
Bst4CI ACNGT 3 cut(s) 56, 710, 730
Bst6I CTCTTC 1 cut(s) 426
BstACI GRCGYC 2 cut(s) 23, 105
BstAPI GCANNNNNTGC 1 cut(s) 542
BstDEI CTNAG 1 cut(s) 324
BstDSI CCRYGG 1 cut(s) 120
BstF5I GGATG 2 cut(s) 174, 689
BstFNI CGCG 1 cut(s) 159
BstKTI GATC 5 cut(s) 42, 63, 367, 598, 786
BstMAI GTCTC 1 cut(s) 752
BstMBI GATC 5 cut(s) 39, 60, 364, 595, 783
BstMWI GCNNNNNNNGC 3 cut(s) 116, 217, 542
BstNI CCWGG 1 cut(s) 137
BstNSI RCATGY 1 cut(s) 277
BstPAI GACNNNNGTC 1 cut(s) 98
BstSCI CCNGG 3 cut(s) 44, 135, 150
BstUI CGCG 1 cut(s) 159
BstV1I GCAGC 2 cut(s) 229, 556
BstX2I RGATCY 2 cut(s) 60, 364
BstYI RGATCY 2 cut(s) 60, 364
Bsu15I ATCGAT 1 cut(s) 598
BsuI GTATCC 1 cut(s) 286
BsuRI GGCC 2 cut(s) 119, 140
BsuTUI ATCGAT 1 cut(s) 598
BtgI CCRYGG 1 cut(s) 120
BtgZI GCGATG 1 cut(s) 192
BtsCI GGATG 2 cut(s) 174, 689
BtsIMutI CAGTG 1 cut(s) 715
BtuMI TCGCGA 1 cut(s) 159
Cfr13I GGNCC 4 cut(s) 133, 148, 345, 686
ClaI ATCGAT 1 cut(s) 598
CseI GACGC 3 cut(s) 12, 70, 113
Csp6I GTAC 1 cut(s) 194
CviAII CATG 7 cut(s) 121, 260, 274, 484, 532, 638, 679
CviQI GTAC 1 cut(s) 194
DdeI CTNAG 1 cut(s) 324
DpnI GATC 5 cut(s) 41, 62, 366, 597, 785
DpnII GATC 5 cut(s) 39, 60, 364, 595, 783
Eam1104I CTCTTC 1 cut(s) 426
EarI CTCTTC 1 cut(s) 426
Ecl136II GAGCTC 1 cut(s) 70
Eco130I CCWWGG 2 cut(s) 120, 742
Eco24I GRGCYC 1 cut(s) 72
Eco47I GGWCC 4 cut(s) 133, 148, 345, 686
Eco53kI GAGCTC 1 cut(s) 70
Eco57I CTGAAG 4 cut(s) 113, 233, 450, 567
EcoICRI GAGCTC 1 cut(s) 70
EcoO109I RGGNCCY 1 cut(s) 345
EcoRII CCWGG 1 cut(s) 135
EcoT14I CCWWGG 2 cut(s) 120, 742
EcoT22I ATGCAT 2 cut(s) 208, 639
EcoT38I GRGCYC 1 cut(s) 72
ErhI CCWWGG 2 cut(s) 120, 742
FaeI CATG 7 cut(s) 124, 263, 277, 487, 535, 641, 682
FaqI GGGAC 2 cut(s) 358, 699
FatI CATG 7 cut(s) 120, 259, 273, 483, 531, 637, 678
FauNDI CATATG 1 cut(s) 541
FbaI TGATCA 1 cut(s) 783
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 4 cut(s) 108, 218, 377, 570
FokI GGATG 2 cut(s) 161, 676
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 4 cut(s) 108, 218, 377, 570
FspBI CTAG 2 cut(s) 170, 503
GluI GCNGC 4 cut(s) 108, 218, 377, 570
HaeIII GGCC 2 cut(s) 119, 140
HapII CCGG 3 cut(s) 45, 151, 266
HgaI GACGC 3 cut(s) 12, 70, 113
Hin1I GRCGYC 2 cut(s) 23, 105
Hin1II CATG 7 cut(s) 124, 263, 277, 487, 535, 641, 682
HincII GTYRAC 2 cut(s) 103, 514
HindII GTYRAC 2 cut(s) 103, 514
HindIII AAGCTT 2 cut(s) 353, 698
HinfI GANTC 2 cut(s) 32, 448
HpaII CCGG 3 cut(s) 45, 151, 266
HphI GGTGA 3 cut(s) 76, 731, 793
Hpy166II GTNNAC 2 cut(s) 103, 514
Hpy188I TCNGA 3 cut(s) 60, 67, 616
Hpy188III TCNNGA 5 cut(s) 158, 266, 314, 461, 668
Hpy8I GTNNAC 2 cut(s) 103, 514
Hpy99I CGWCG 4 cut(s) 28, 104, 107, 164
HpyAV CCTTC 9 cut(s) 109, 216, 226, 355, 358, 486, 502, 676, 743
HpyCH4III ACNGT 3 cut(s) 56, 710, 730
HpyCH4V TGCA 4 cut(s) 206, 371, 477, 637
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 217, 542
HpyF3I CTNAG 1 cut(s) 324
Hsp92I GRCGYC 2 cut(s) 23, 105
Hsp92II CATG 7 cut(s) 124, 263, 277, 487, 535, 641, 682
Kpn2I TCCGGA 1 cut(s) 265
Ksp22I TGATCA 1 cut(s) 783
Kzo9I GATC 5 cut(s) 39, 60, 364, 595, 783
LguI GCTCTTC 1 cut(s) 426
LmnI GCTCC 4 cut(s) 67, 75, 124, 577
Lsp1109I GCAGC 2 cut(s) 229, 556
LweI GCATC 2 cut(s) 193, 215
MaeI CTAG 2 cut(s) 170, 503
MaeIII GTNAC 2 cut(s) 82, 737
MalI GATC 5 cut(s) 41, 62, 366, 597, 785
MboI GATC 5 cut(s) 39, 60, 364, 595, 783
MboII GAAGA 2 cut(s) 443, 592
MflI RGATCY 2 cut(s) 60, 364
MhlI GDGCHC 1 cut(s) 72
MluCI AATT 3 cut(s) 227, 286, 553
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 2 cut(s) 9, 38
MnlI CCTC 4 cut(s) 482, 622, 798, 807
Mph1103I ATGCAT 2 cut(s) 208, 639
MroI TCCGGA 1 cut(s) 265
MseI TTAA 2 cut(s) 525, 752
MslI CAYNNNNRTG 2 cut(s) 278, 482
MspI CCGG 3 cut(s) 45, 151, 266
MspR9I CCNGG 3 cut(s) 46, 137, 152
Mva1269I GAATGC 1 cut(s) 446
MvaI CCWGG 1 cut(s) 137
MvnI CGCG 1 cut(s) 159
MwoI GCNNNNNNNGC 3 cut(s) 116, 217, 542
NciI CCSGG 2 cut(s) 46, 152
NcoI CCATGG 1 cut(s) 120
NdeI CATATG 1 cut(s) 541
NdeII GATC 5 cut(s) 39, 60, 364, 595, 783
NlaIII CATG 7 cut(s) 124, 263, 277, 487, 535, 641, 682
NlaIV GGNNCC 3 cut(s) 346, 366, 687
NmuCI GTSAC 2 cut(s) 82, 737
NruI TCGCGA 1 cut(s) 159
NsiI ATGCAT 2 cut(s) 208, 639
NspI RCATGY 1 cut(s) 277
PciSI GCTCTTC 1 cut(s) 426
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PctI GAATGC 1 cut(s) 446
PfeI GAWTC 1 cut(s) 448
PflFI GACNNNGTC 1 cut(s) 80
PkrI GCNGC 4 cut(s) 109, 219, 378, 571
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PpuMI RGGWCCY 1 cut(s) 345
PshAI GACNNNNGTC 1 cut(s) 98
PshBI ATTAAT 1 cut(s) 525
Psp124BI GAGCTC 1 cut(s) 72
Psp5II RGGWCCY 1 cut(s) 345
Psp6I CCWGG 1 cut(s) 135
PspGI CCWGG 1 cut(s) 135
PspN4I GGNNCC 3 cut(s) 346, 366, 687
PspPI GGNCC 4 cut(s) 133, 148, 345, 686
PspPPI RGGWCCY 1 cut(s) 345
PsuI RGATCY 2 cut(s) 60, 364
PsyI GACNNNGTC 1 cut(s) 80
RruI TCGCGA 1 cut(s) 159
RsaI GTAC 1 cut(s) 195
RsaNI GTAC 1 cut(s) 194
RseI CAYNNNNRTG 2 cut(s) 278, 482
SacI GAGCTC 1 cut(s) 72
SalI GTCGAC 1 cut(s) 101
SapI GCTCTTC 1 cut(s) 426
SaqAI TTAA 2 cut(s) 525, 752
SatI GCNGC 4 cut(s) 108, 218, 377, 570
Sau3AI GATC 5 cut(s) 39, 60, 364, 595, 783
Sau96I GGNCC 4 cut(s) 133, 148, 345, 686
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 3 cut(s) 46, 137, 152
SduI GDGCHC 1 cut(s) 72
SfaNI GCATC 2 cut(s) 193, 215
SgrDI CGTCGACG 1 cut(s) 101
SinI GGWCC 4 cut(s) 133, 148, 345, 686
SmiMI CAYNNNNRTG 2 cut(s) 278, 482
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
Sse9I AATT 3 cut(s) 227, 286, 553
SsiI CCGC 3 cut(s) 108, 374, 377
SspMI CTAG 2 cut(s) 170, 503
SstI GAGCTC 1 cut(s) 72
StyD4I CCNGG 3 cut(s) 44, 135, 150
StyI CCWWGG 2 cut(s) 120, 742
TaaI ACNGT 3 cut(s) 56, 710, 730
TaqI TCGA 3 cut(s) 102, 598, 669
TasI AATT 3 cut(s) 227, 286, 553
TauI GCSGC 2 cut(s) 110, 379
TfiI GAWTC 1 cut(s) 448
Tru1I TTAA 2 cut(s) 525, 752
Tru9I TTAA 2 cut(s) 525, 752
TscAI CASTG 1 cut(s) 715
TseFI GTSAC 2 cut(s) 82, 737
TseI GCWGC 2 cut(s) 217, 569
Tsp45I GTSAC 2 cut(s) 82, 737
TspDTI ATGAA 2 cut(s) 654, 695
TspGWI ACGGA 2 cut(s) 88, 778
TspRI CASTG 1 cut(s) 715
Tth111I GACNNNGTC 1 cut(s) 80
VpaK11BI GGWCC 4 cut(s) 133, 148, 345, 686
VspI ATTAAT 1 cut(s) 525
XapI RAATTY 1 cut(s) 286
XceI RCATGY 1 cut(s) 277
XcmI CCANNNNNNNNNTGG 1 cut(s) 187
XmiI GTMKAC 1 cut(s) 102
XspI CTAG 2 cut(s) 170, 503
Zsp2I ATGCAT 2 cut(s) 208, 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.