RchiOBHm_Chr1g0349231

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001324
Physical Location & Seq
Forward (+)
42347758 .. 42350613
2856 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57521

Sequence Viewer

Length: 705 bp
ATGATGTCAAAGATTAAGGGAAATTTGTTCAAAAGTTTGGGTGAAGTTTGTGATGATTGTTCGGCTACTGCTTGTATAATTAGATGGTACGATGTGATGGCAGAAACTGATCGATGCATCGCTAAGCAGCAAAGGAAATTGAAGGATATGTCAATTTATGCCCCCACTCATGTTCCTGAAAGAATGTCGATGTTGACTTTGGACACAAATAGATGTTTATTTCCAGAAAGCTCTCAGCAGCAAAACGCTGGTTCTGGGACCTTCAAGCTTGAGGAGGATCCTGCACCACCACCCCCACCCCCACCCAAGGAGAAAAAGGGTCGTTCCACGCCACCATTGTGGTTTATAAGTGCTGAAGAGCTGGATTCCTTGTCATCATACATGAAAGGAAGGCTTACACTAGAGAAGGTCAATGCAGCTATTAGTGACATGGCAACATATGCTGAAGCGAATTATCAGCTCATAACTGCTCCAAAGAAGAATGTGGCAGGAGATCGATGGGAAAAAGCCTCGGAAATAAGAGATGTAGCAATGCATGAAGCAGTAAAGGGAAAACACTTCTTTCTCGAAACTGACATGAAGGGACCATCCTTGAAGCTTGACAACTCTGGAAAAGCAATACTAACTGTCCTTCGTCACCTTGGTTGTGTAAGTGAGGCACGGATTGGTCATCATCGGGTGATCATTCTTTTGAAGCCTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

234

Amino Acids

26.14

Weight (kDa)

8.66

Isoelectric Point (pI)

54.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 38 - 224 2.1e-58 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 347
AclWI GGATC 2 cut(s) 272, 285
AcsI RAATTY 1 cut(s) 22
AcuI CTGAAG 2 cut(s) 375, 465
AfaI GTAC 1 cut(s) 89
AfiI CCNNNNNNNGG 1 cut(s) 307
AgsI TTSAA 5 cut(s) 31, 142, 265, 595, 694
AleI CACNNNNGTG 1 cut(s) 337
AluBI AGCT 6 cut(s) 231, 268, 361, 419, 460, 598
AluI AGCT 6 cut(s) 231, 268, 361, 419, 460, 598
AlwI GGATC 2 cut(s) 272, 285
AlwNI CAGNNNCTG 1 cut(s) 107
ApeKI GCWGC 3 cut(s) 127, 238, 416
ApoI RAATTY 1 cut(s) 22
AspS9I GGNCC 2 cut(s) 258, 584
AsuHPI GGTGA 3 cut(s) 53, 629, 691
AvaII GGWCC 2 cut(s) 258, 584
BamHI GGATCC 1 cut(s) 277
BbvI GCAGC 3 cut(s) 139, 250, 428
BccI CCATC 4 cut(s) 78, 91, 492, 595
BclI TGATCA 1 cut(s) 681
BfaI CTAG 1 cut(s) 401
BisI GCNGC 3 cut(s) 128, 239, 417
BlpI GCTNAGC 1 cut(s) 123
BlsI GCNGC 3 cut(s) 129, 240, 418
Bme18I GGWCC 2 cut(s) 258, 584
BmgT120I GGNCC 2 cut(s) 258, 584
BmiI GGNNCC 3 cut(s) 259, 279, 585
BmsI GCATC 2 cut(s) 104, 126
Bpu1102I GCTNAGC 1 cut(s) 123
BpuEI CTTGAG 1 cut(s) 290
Bsa29I ATCGAT 2 cut(s) 112, 496
BsaJI CCNNGG 3 cut(s) 306, 510, 640
Bsc4I CCNNNNNNNGG 1 cut(s) 307
Bse3DI GCAATG 1 cut(s) 537
BseCI ATCGAT 2 cut(s) 112, 496
BseDI CCNNGG 3 cut(s) 306, 510, 640
BseGI GGATG 1 cut(s) 587
BseLI CCNNNNNNNGG 1 cut(s) 307
BseMI GCAATG 1 cut(s) 537
BseMII CTCAG 1 cut(s) 248
BseRI GAGGAG 1 cut(s) 287
BseXI GCAGC 3 cut(s) 139, 250, 428
BsgI GTGCAG 1 cut(s) 267
BshVI ATCGAT 2 cut(s) 112, 496
BslFI GGGAC 2 cut(s) 271, 597
BslI CCNNNNNNNGG 1 cut(s) 307
BsmFI GGGAC 2 cut(s) 271, 597
Bsp143I GATC 4 cut(s) 109, 277, 493, 681
Bsp1720I GCTNAGC 1 cut(s) 123
BspCNI CTCAG 1 cut(s) 247
BspDI ATCGAT 2 cut(s) 112, 496
BspLI GGNNCC 3 cut(s) 259, 279, 585
BspPI GGATC 2 cut(s) 272, 285
BspQI GCTCTTC 1 cut(s) 351
BsrDI GCAATG 1 cut(s) 537
BssECI CCNNGG 3 cut(s) 306, 510, 640
BssMI GATC 4 cut(s) 109, 277, 493, 681
BssT1I CCWWGG 2 cut(s) 306, 640
Bst4CI ACNGT 1 cut(s) 628
Bst6I CTCTTC 1 cut(s) 351
BstAPI GCANNNNNTGC 1 cut(s) 440
BstDEI CTNAG 2 cut(s) 123, 234
BstF5I GGATG 1 cut(s) 587
BstKTI GATC 4 cut(s) 112, 280, 496, 684
BstMBI GATC 4 cut(s) 109, 277, 493, 681
BstMWI GCNNNNNNNGC 1 cut(s) 440
BstV1I GCAGC 3 cut(s) 139, 250, 428
BstX2I RGATCY 1 cut(s) 277
BstXI CCANNNNNNTGG 1 cut(s) 339
BstYI RGATCY 1 cut(s) 277
Bsu15I ATCGAT 2 cut(s) 112, 496
BsuTUI ATCGAT 2 cut(s) 112, 496
BtgZI GCGATG 1 cut(s) 103
BtsCI GGATG 1 cut(s) 587
BtsIMutI CAGTG 1 cut(s) 700
CaiI CAGNNNCTG 1 cut(s) 107
Cfr13I GGNCC 2 cut(s) 258, 584
ClaI ATCGAT 2 cut(s) 112, 496
Csp6I GTAC 1 cut(s) 88
CviAII CATG 5 cut(s) 170, 382, 430, 536, 577
CviQI GTAC 1 cut(s) 88
DdeI CTNAG 2 cut(s) 123, 234
DpnI GATC 4 cut(s) 111, 279, 495, 683
DpnII GATC 4 cut(s) 109, 277, 493, 681
Eam1104I CTCTTC 1 cut(s) 351
EarI CTCTTC 1 cut(s) 351
Eco130I CCWWGG 2 cut(s) 306, 640
Eco47I GGWCC 2 cut(s) 258, 584
Eco57I CTGAAG 2 cut(s) 375, 465
EcoO109I RGGNCCY 1 cut(s) 258
EcoT14I CCWWGG 2 cut(s) 306, 640
EcoT22I ATGCAT 2 cut(s) 119, 537
ErhI CCWWGG 2 cut(s) 306, 640
FaeI CATG 5 cut(s) 173, 385, 433, 539, 580
FalI AAGNNNNNCTT 2 cut(s) 378, 410
FaqI GGGAC 2 cut(s) 271, 597
FatI CATG 5 cut(s) 169, 381, 429, 535, 576
FauNDI CATATG 1 cut(s) 439
FbaI TGATCA 1 cut(s) 681
Fnu4HI GCNGC 3 cut(s) 128, 239, 417
FokI GGATG 1 cut(s) 574
Fsp4HI GCNGC 3 cut(s) 128, 239, 417
FspBI CTAG 1 cut(s) 401
GluI GCNGC 3 cut(s) 128, 239, 417
Hin1II CATG 5 cut(s) 173, 385, 433, 539, 580
HincII GTYRAC 1 cut(s) 195
HindII GTYRAC 1 cut(s) 195
HindIII AAGCTT 2 cut(s) 266, 596
HinfI GANTC 1 cut(s) 365
HphI GGTGA 3 cut(s) 53, 629, 691
Hpy166II GTNNAC 1 cut(s) 195
Hpy188I TCNGA 1 cut(s) 514
Hpy188III TCNNGA 4 cut(s) 176, 224, 566, 609
Hpy8I GTNNAC 1 cut(s) 195
HpyAV CCTTC 6 cut(s) 136, 271, 384, 400, 574, 641
HpyCH4III ACNGT 1 cut(s) 628
HpyCH4V TGCA 4 cut(s) 117, 284, 416, 535
HpyF10VI GCNNNNNNNGC 1 cut(s) 440
HpyF3I CTNAG 2 cut(s) 123, 234
Hsp92II CATG 5 cut(s) 173, 385, 433, 539, 580
Ksp22I TGATCA 1 cut(s) 681
Kzo9I GATC 4 cut(s) 109, 277, 493, 681
LguI GCTCTTC 1 cut(s) 351
LmnI GCTCC 1 cut(s) 475
LpnPI CCDG 8 cut(s) 189, 234, 237, 240, 294, 347, 474, 594
Lsp1109I GCAGC 3 cut(s) 139, 250, 428
LweI GCATC 2 cut(s) 104, 126
MaeI CTAG 1 cut(s) 401
MaeIII GTNAC 2 cut(s) 425, 635
MalI GATC 4 cut(s) 111, 279, 495, 683
MboI GATC 4 cut(s) 109, 277, 493, 681
MboII GAAGA 2 cut(s) 368, 490
MflI RGATCY 1 cut(s) 277
MluCI AATT 5 cut(s) 22, 78, 137, 153, 451
MnlI CCTC 4 cut(s) 265, 268, 520, 649
Mph1103I ATGCAT 2 cut(s) 119, 537
MseI TTAA 1 cut(s) 15
MslI CAYNNNNRTG 1 cut(s) 337
MwoI GCNNNNNNNGC 1 cut(s) 440
NdeI CATATG 1 cut(s) 439
NdeII GATC 4 cut(s) 109, 277, 493, 681
NlaIII CATG 5 cut(s) 173, 385, 433, 539, 580
NlaIV GGNNCC 3 cut(s) 259, 279, 585
NmuCI GTSAC 2 cut(s) 425, 635
NsiI ATGCAT 2 cut(s) 119, 537
OliI CACNNNNGTG 1 cut(s) 337
PciSI GCTCTTC 1 cut(s) 351
PfeI GAWTC 1 cut(s) 365
PkrI GCNGC 3 cut(s) 129, 240, 418
PpuMI RGGWCCY 1 cut(s) 258
PsiI TTATAA 1 cut(s) 347
Psp5II RGGWCCY 1 cut(s) 258
PspN4I GGNNCC 3 cut(s) 259, 279, 585
PspPI GGNCC 2 cut(s) 258, 584
PspPPI RGGWCCY 1 cut(s) 258
PstNI CAGNNNCTG 1 cut(s) 107
PsuI RGATCY 1 cut(s) 277
RsaI GTAC 1 cut(s) 89
RsaNI GTAC 1 cut(s) 88
RseI CAYNNNNRTG 1 cut(s) 337
SapI GCTCTTC 1 cut(s) 351
SaqAI TTAA 1 cut(s) 15
SatI GCNGC 3 cut(s) 128, 239, 417
Sau3AI GATC 4 cut(s) 109, 277, 493, 681
Sau96I GGNCC 2 cut(s) 258, 584
SetI ASST 9 cut(s) 233, 263, 270, 363, 411, 421, 462, 600, 642
SfaNI GCATC 2 cut(s) 104, 126
SinI GGWCC 2 cut(s) 258, 584
SmiMI CAYNNNNRTG 1 cut(s) 337
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
Sse9I AATT 5 cut(s) 22, 78, 137, 153, 451
SspMI CTAG 1 cut(s) 401
StyI CCWWGG 2 cut(s) 306, 640
TaaI ACNGT 1 cut(s) 628
TaqI TCGA 4 cut(s) 112, 188, 496, 567
TasI AATT 5 cut(s) 22, 78, 137, 153, 451
TfiI GAWTC 1 cut(s) 365
Tru1I TTAA 1 cut(s) 15
Tru9I TTAA 1 cut(s) 15
TseFI GTSAC 2 cut(s) 425, 635
TseI GCWGC 3 cut(s) 127, 238, 416
Tsp45I GTSAC 2 cut(s) 425, 635
TspDTI ATGAA 3 cut(s) 398, 552, 593
TspGWI ACGGA 1 cut(s) 676
VpaK11BI GGWCC 2 cut(s) 258, 584
XapI RAATTY 1 cut(s) 22
XspI CTAG 1 cut(s) 401
Zsp2I ATGCAT 2 cut(s) 119, 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.