Rmu_sc0002914.1_g000030

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002914.1
OG0001324
Physical Location & Seq
Reverse (-)
137040 .. 137422
383 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002914.1_g000030.1.cds

Sequence Viewer

Length: 285 bp
atggaggctgagaaagaaggggcatcgttggactcgctgatcgccgggttcaacagttggatctcggagctccaagagctcgttattgggcgcaacatgtacccggccagcagcgtcacagatttgacggccgtcgacgccgccgtgaaggccatggagcttcaggtccaggccatcaacaaccgggttcgcgacgaaaccctagccatccccaaagcacaagtagttccttctctctctctctctctatatgagtatgtggattgggctttgtttaatttctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

94

Amino Acids

10.36

Weight (kDa)

4.25

Isoelectric Point (pI)

25.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 135
AccII CGCG 1 cut(s) 192
AciI CCGC 1 cut(s) 141
AclWI GGATC 1 cut(s) 68
AcoI YGGCCR 2 cut(s) 105, 129
AcuI CTGAAG 1 cut(s) 146
AcyI GRCGYC 1 cut(s) 138
AfaI GTAC 1 cut(s) 101
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 1 cut(s) 52
AjnI CCWGG 1 cut(s) 168
AluBI AGCT 3 cut(s) 70, 79, 160
AluI AGCT 3 cut(s) 70, 79, 160
Alw21I GWGCWC 2 cut(s) 72, 81
AlwI GGATC 1 cut(s) 68
AoxI GGCC 4 cut(s) 105, 129, 150, 171
ApeKI GCWGC 1 cut(s) 111
AspLEI GCGC 1 cut(s) 93
AspS9I GGNCC 1 cut(s) 166
AsuC2I CCSGG 3 cut(s) 46, 104, 185
AvaII GGWCC 1 cut(s) 166
BanII GRGCYC 2 cut(s) 72, 81
Bbv12I GWGCWC 2 cut(s) 72, 81
BbvI GCAGC 1 cut(s) 123
BccI CCATC 2 cut(s) 182, 215
BceAI ACGGC 3 cut(s) 116, 128, 144
BciT130I CCWGG 1 cut(s) 170
BcnI CCSGG 3 cut(s) 46, 104, 185
BfaI CTAG 1 cut(s) 203
BglI GCCNNNNNGGC 1 cut(s) 149
BisI GCNGC 2 cut(s) 112, 141
BlsI GCNGC 2 cut(s) 113, 142
Bme1390I CCNGG 4 cut(s) 46, 104, 170, 185
Bme18I GGWCC 1 cut(s) 166
BmgT120I GGNCC 1 cut(s) 166
BmrFI CCNGG 4 cut(s) 46, 104, 170, 185
BmsI GCATC 1 cut(s) 32
BoxI GACNNNNGTC 1 cut(s) 131
BpuMI CCSGG 3 cut(s) 46, 104, 185
BsaHI GRCGYC 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 153
BseBI CCWGG 1 cut(s) 170
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 1 cut(s) 207
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 1 cut(s) 123
Bsh1236I CGCG 1 cut(s) 192
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 4 cut(s) 107, 131, 152, 173
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 2 cut(s) 72, 81
BsiSI CCGG 3 cut(s) 45, 104, 184
BsnI GGCC 4 cut(s) 107, 131, 152, 173
Bsp1286I GDGCHC 2 cut(s) 72, 81
Bsp143I GATC 2 cut(s) 39, 60
Bsp19I CCATGG 1 cut(s) 153
Bsp68I TCGCGA 1 cut(s) 192
BspACI CCGC 1 cut(s) 141
BspANI GGCC 4 cut(s) 107, 131, 152, 173
BspFNI CGCG 1 cut(s) 192
BspPI GGATC 1 cut(s) 68
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 2 cut(s) 39, 60
BssNI GRCGYC 1 cut(s) 138
BssT1I CCWWGG 1 cut(s) 153
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 1 cut(s) 56
BstACI GRCGYC 1 cut(s) 138
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 1 cut(s) 9
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 1 cut(s) 207
BstFNI CGCG 1 cut(s) 192
BstHHI GCGC 1 cut(s) 93
BstKTI GATC 2 cut(s) 42, 63
BstMBI GATC 2 cut(s) 39, 60
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 3 cut(s) 76, 137, 149
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 100
BstPAI GACNNNNGTC 1 cut(s) 131
BstSCI CCNGG 4 cut(s) 44, 102, 168, 183
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 1 cut(s) 123
BstX2I RGATCY 1 cut(s) 60
BstYI RGATCY 1 cut(s) 60
BstZI CGGCCG 1 cut(s) 129
BsuRI GGCC 4 cut(s) 107, 131, 152, 173
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 1 cut(s) 207
BtuMI TCGCGA 1 cut(s) 192
Cac8I GCNNGC 1 cut(s) 109
CfoI GCGC 1 cut(s) 93
Cfr13I GGNCC 1 cut(s) 166
CseI GACGC 2 cut(s) 103, 146
Csp6I GTAC 1 cut(s) 100
CviAII CATG 2 cut(s) 97, 154
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 1 cut(s) 9
DpnI GATC 2 cut(s) 41, 62
DpnII GATC 2 cut(s) 39, 60
EaeI YGGCCR 2 cut(s) 105, 129
EagI CGGCCG 1 cut(s) 129
Ecl136II GAGCTC 2 cut(s) 70, 79
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 153
Eco24I GRGCYC 2 cut(s) 72, 81
Eco47I GGWCC 1 cut(s) 166
Eco52I CGGCCG 1 cut(s) 129
Eco53kI GAGCTC 2 cut(s) 70, 79
Eco57I CTGAAG 1 cut(s) 146
EcoICRI GAGCTC 2 cut(s) 70, 79
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 1 cut(s) 153
EcoT38I GRGCYC 2 cut(s) 72, 81
ErhI CCWWGG 1 cut(s) 153
FaeI CATG 2 cut(s) 100, 157
FaiI YATR 5 cut(s) 98, 155, 250, 252, 258
FatI CATG 2 cut(s) 96, 153
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 2 cut(s) 112, 141
FokI GGATG 1 cut(s) 194
FriOI GRGCYC 2 cut(s) 72, 81
Fsp4HI GCNGC 2 cut(s) 112, 141
FspBI CTAG 1 cut(s) 203
GlaI GCGC 1 cut(s) 92
GluI GCNGC 2 cut(s) 112, 141
HaeIII GGCC 4 cut(s) 107, 131, 152, 173
HapII CCGG 3 cut(s) 45, 104, 184
HgaI GACGC 2 cut(s) 103, 146
HhaI GCGC 1 cut(s) 93
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 2 cut(s) 100, 157
Hin6I GCGC 1 cut(s) 91
HinP1I GCGC 1 cut(s) 91
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HinfI GANTC 1 cut(s) 32
HpaII CCGG 3 cut(s) 45, 104, 184
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 2 cut(s) 67, 284
Hpy188III TCNNGA 1 cut(s) 191
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 3 cut(s) 137, 140, 197
HpyAV CCTTC 3 cut(s) 11, 142, 240
HpyCH4III ACNGT 1 cut(s) 56
HpyF10VI GCNNNNNNNGC 3 cut(s) 76, 137, 149
HpyF3I CTNAG 1 cut(s) 9
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 2 cut(s) 100, 157
HspAI GCGC 1 cut(s) 91
Kzo9I GATC 2 cut(s) 39, 60
LmnI GCTCC 3 cut(s) 67, 75, 157
LpnPI CCDG 7 cut(s) 58, 117, 121, 149, 155, 182, 197
Lsp1109I GCAGC 1 cut(s) 123
LweI GCATC 1 cut(s) 32
MaeI CTAG 1 cut(s) 203
MaeIII GTNAC 1 cut(s) 115
MalI GATC 2 cut(s) 41, 62
MboI GATC 2 cut(s) 39, 60
MflI RGATCY 1 cut(s) 60
MhlI GDGCHC 2 cut(s) 72, 81
MluCI AATT 1 cut(s) 277
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 2 cut(s) 9, 38
MseI TTAA 1 cut(s) 276
MspI CCGG 3 cut(s) 45, 104, 184
MspR9I CCNGG 4 cut(s) 46, 104, 170, 185
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 1 cut(s) 192
MwoI GCNNNNNNNGC 3 cut(s) 76, 137, 149
NciI CCSGG 3 cut(s) 46, 104, 185
NcoI CCATGG 1 cut(s) 153
NdeII GATC 2 cut(s) 39, 60
NlaIII CATG 2 cut(s) 100, 157
NmuCI GTSAC 1 cut(s) 115
NruI TCGCGA 1 cut(s) 192
NspI RCATGY 1 cut(s) 100
PciI ACATGT 1 cut(s) 96
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PkrI GCNGC 2 cut(s) 113, 142
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PscI ACATGT 1 cut(s) 96
PshAI GACNNNNGTC 1 cut(s) 131
Psp124BI GAGCTC 2 cut(s) 72, 81
Psp6I CCWGG 1 cut(s) 168
PspGI CCWGG 1 cut(s) 168
PspPI GGNCC 1 cut(s) 166
PsuI RGATCY 1 cut(s) 60
RruI TCGCGA 1 cut(s) 192
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SacI GAGCTC 2 cut(s) 72, 81
SalI GTCGAC 1 cut(s) 134
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 2 cut(s) 112, 141
Sau3AI GATC 2 cut(s) 39, 60
Sau96I GGNCC 1 cut(s) 166
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 4 cut(s) 46, 104, 170, 185
SduI GDGCHC 2 cut(s) 72, 81
SetI ASST 4 cut(s) 72, 81, 162, 168
SfaNI GCATC 1 cut(s) 32
SgrDI CGTCGACG 1 cut(s) 134
SinI GGWCC 1 cut(s) 166
Sse9I AATT 1 cut(s) 277
SsiI CCGC 1 cut(s) 141
SspMI CTAG 1 cut(s) 203
SstI GAGCTC 2 cut(s) 72, 81
StyD4I CCNGG 4 cut(s) 44, 102, 168, 183
StyI CCWWGG 1 cut(s) 153
TaaI ACNGT 1 cut(s) 56
TaqI TCGA 1 cut(s) 135
TasI AATT 1 cut(s) 277
TauI GCSGC 1 cut(s) 143
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TseFI GTSAC 1 cut(s) 115
TseI GCWGC 1 cut(s) 111
Tsp45I GTSAC 1 cut(s) 115
VpaK11BI GGWCC 1 cut(s) 166
XceI RCATGY 1 cut(s) 100
XmiI GTMKAC 1 cut(s) 135
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.