RLG00000028595

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001324
Physical Location & Seq
Reverse (-)
24832105 .. 24834361
2257 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028595

Sequence Viewer

Length: 555 bp
ATGGAGGCTGAGAAAGCAGGAGCGTCGTTGGACTCGCTGATCACTGGTACCGTCACCGATTTGACGGCCGTCGACACCGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGCCATCAAGGACCTGGTTCGCGACGAAACCCTCGCCATCCCCAAAGCAAAAAAACTGATCGATGCATCGCTGAAGCAGCAAAGGAAATTGAAGGACATGTCAATCACTCATGATCCTGAAAGGATGTCAATGTTGAATTTGGATACAAATAGATGTTTATTTCCAGATAGCTCTCAGCAAAACACTGGTTCTGGGACCTTCAAGCTTGATGAGGATCCTGCACCACCACCAGAGAAAAAGGGTCGTTCCACGTCACCATTGTGGTTTATAAGTGCTGAAGAGCTGAATTCCTTGTCACCATACATGAGAGGAAGGCTTACACTAGAGAAGGTCAATGCAGCTATTAGTGACATGGCAACATATGCTGAAGCAAATTATCAGCCCGTAGCTGCTCCAAAGAAGAATGTGGCAGGAGATCGATGGGAAAAAGCCTTGGTAAGTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

185

Amino Acids

20.18

Weight (kDa)

5.94

Isoelectric Point (pI)

41.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 18 - 181 1.2e-38 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 377
Acc65I GGTACC 1 cut(s) 47
AccB1I GGYRCC 1 cut(s) 47
AccI GTMKAC 1 cut(s) 72
AccII CGCG 1 cut(s) 129
AciI CCGC 1 cut(s) 78
AclWI GGATC 3 cut(s) 215, 317, 330
AcoI YGGCCR 1 cut(s) 66
AcsI RAATTY 2 cut(s) 244, 394
AcuI CTGAAG 4 cut(s) 83, 200, 405, 495
AfaI GTAC 1 cut(s) 49
AflIII ACRYGT 1 cut(s) 204
AgsI TTSAA 3 cut(s) 199, 244, 310
AjiI CACGTC 1 cut(s) 360
AjnI CCWGG 2 cut(s) 105, 120
AleI CACNNNNGTG 2 cut(s) 80, 367
AluBI AGCT 6 cut(s) 97, 279, 313, 391, 449, 497
AluI AGCT 6 cut(s) 97, 279, 313, 391, 449, 497
AlwI GGATC 3 cut(s) 215, 317, 330
AoxI GGCC 3 cut(s) 66, 87, 108
ApeKI GCWGC 3 cut(s) 184, 446, 497
ApoI RAATTY 2 cut(s) 244, 394
Asp718I GGTACC 1 cut(s) 47
AspS9I GGNCC 3 cut(s) 103, 118, 303
AsuHPI GGTGA 3 cut(s) 46, 354, 396
AvaII GGWCC 3 cut(s) 103, 118, 303
BamHI GGATCC 1 cut(s) 322
BanI GGYRCC 1 cut(s) 47
BbvI GCAGC 3 cut(s) 196, 458, 484
BccI CCATC 3 cut(s) 119, 152, 522
BceAI ACGGC 3 cut(s) 53, 65, 81
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BciT130I CCWGG 2 cut(s) 107, 122
BciVI GTATCC 1 cut(s) 244
BclI TGATCA 1 cut(s) 39
BfaI CTAG 1 cut(s) 431
BfuI GTATCC 1 cut(s) 244
BglI GCCNNNNNGGC 1 cut(s) 86
BisI GCNGC 3 cut(s) 185, 447, 498
BlsI GCNGC 3 cut(s) 186, 448, 499
Bme1390I CCNGG 2 cut(s) 107, 122
Bme18I GGWCC 3 cut(s) 103, 118, 303
BmgBI CACGTC 1 cut(s) 360
BmgT120I GGNCC 3 cut(s) 103, 118, 303
BmiI GGNNCC 3 cut(s) 49, 304, 324
BmrFI CCNGG 2 cut(s) 107, 122
BmsI GCATC 2 cut(s) 160, 182
BoxI GACNNNNGTC 1 cut(s) 68
Bsa29I ATCGAT 2 cut(s) 168, 526
BsaBI GATNNNNATC 1 cut(s) 321
BsaJI CCNNGG 2 cut(s) 90, 540
Bse1I ACTGG 2 cut(s) 49, 298
Bse8I GATNNNNATC 1 cut(s) 321
BseBI CCWGG 2 cut(s) 107, 122
BseCI ATCGAT 2 cut(s) 168, 526
BseDI CCNNGG 2 cut(s) 90, 540
BseGI GGATG 2 cut(s) 144, 237
BseJI GATNNNNATC 1 cut(s) 321
BseMII CTCAG 1 cut(s) 296
BseNI ACTGG 2 cut(s) 49, 298
BseX3I CGGCCG 1 cut(s) 66
BseXI GCAGC 3 cut(s) 196, 458, 484
BsgI GTGCAG 1 cut(s) 312
Bsh1236I CGCG 1 cut(s) 129
Bsh1285I CGRYCG 1 cut(s) 69
BshFI GGCC 3 cut(s) 68, 89, 110
BshNI GGYRCC 1 cut(s) 47
BshVI ATCGAT 2 cut(s) 168, 526
BsiEI CGRYCG 1 cut(s) 69
BslFI GGGAC 1 cut(s) 316
BsmFI GGGAC 1 cut(s) 316
BsnI GGCC 3 cut(s) 68, 89, 110
Bsp143I GATC 5 cut(s) 39, 165, 220, 322, 523
Bsp19I CCATGG 1 cut(s) 90
Bsp68I TCGCGA 1 cut(s) 129
BspACI CCGC 1 cut(s) 78
BspANI GGCC 3 cut(s) 68, 89, 110
BspCNI CTCAG 1 cut(s) 295
BspDI ATCGAT 2 cut(s) 168, 526
BspFNI CGCG 1 cut(s) 129
BspHI TCATGA 1 cut(s) 217
BspLI GGNNCC 3 cut(s) 49, 304, 324
BspPI GGATC 3 cut(s) 215, 317, 330
BspQI GCTCTTC 1 cut(s) 381
BspT107I GGYRCC 1 cut(s) 47
BsrI ACTGG 2 cut(s) 49, 298
BssECI CCNNGG 2 cut(s) 90, 540
BssMI GATC 5 cut(s) 39, 165, 220, 322, 523
BssT1I CCWWGG 2 cut(s) 90, 540
Bst2UI CCWGG 2 cut(s) 107, 122
Bst4CI ACNGT 1 cut(s) 52
Bst6I CTCTTC 1 cut(s) 381
BstAPI GCANNNNNTGC 1 cut(s) 470
BstDEI CTNAG 2 cut(s) 9, 282
BstDSI CCRYGG 1 cut(s) 90
BstF5I GGATG 2 cut(s) 144, 237
BstFNI CGCG 1 cut(s) 129
BstKTI GATC 5 cut(s) 42, 168, 223, 325, 526
BstMBI GATC 5 cut(s) 39, 165, 220, 322, 523
BstMCI CGRYCG 1 cut(s) 69
BstMWI GCNNNNNNNGC 4 cut(s) 14, 86, 184, 470
BstNI CCWGG 2 cut(s) 107, 122
BstNSI RCATGY 1 cut(s) 208
BstPAI GACNNNNGTC 1 cut(s) 68
BstSCI CCNGG 2 cut(s) 105, 120
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 3 cut(s) 196, 458, 484
BstX2I RGATCY 1 cut(s) 322
BstYI RGATCY 1 cut(s) 322
BstZI CGGCCG 1 cut(s) 66
Bsu15I ATCGAT 2 cut(s) 168, 526
BsuI GTATCC 1 cut(s) 244
BsuRI GGCC 3 cut(s) 68, 89, 110
BsuTUI ATCGAT 2 cut(s) 168, 526
BtgI CCRYGG 1 cut(s) 90
BtgZI GCGATG 1 cut(s) 159
BtrI CACGTC 1 cut(s) 360
BtsCI GGATG 2 cut(s) 144, 237
BtsIMutI CAGTG 2 cut(s) 42, 291
BtuMI TCGCGA 1 cut(s) 129
CciI TCATGA 1 cut(s) 217
Cfr13I GGNCC 3 cut(s) 103, 118, 303
ClaI ATCGAT 2 cut(s) 168, 526
CseI GACGC 1 cut(s) 12
CsiI ACCWGGT 1 cut(s) 120
Csp6I GTAC 1 cut(s) 48
CviAII CATG 5 cut(s) 91, 205, 218, 412, 460
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 2 cut(s) 9, 282
DpnI GATC 5 cut(s) 41, 167, 222, 324, 525
DpnII GATC 5 cut(s) 39, 165, 220, 322, 523
EaeI YGGCCR 1 cut(s) 66
EagI CGGCCG 1 cut(s) 66
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
EclXI CGGCCG 1 cut(s) 66
Eco130I CCWWGG 2 cut(s) 90, 540
Eco47I GGWCC 3 cut(s) 103, 118, 303
Eco52I CGGCCG 1 cut(s) 66
Eco57I CTGAAG 4 cut(s) 83, 200, 405, 495
EcoO109I RGGNCCY 2 cut(s) 118, 303
EcoRI GAATTC 1 cut(s) 394
EcoRII CCWGG 2 cut(s) 105, 120
EcoT14I CCWWGG 2 cut(s) 90, 540
EcoT22I ATGCAT 1 cut(s) 175
ErhI CCWWGG 2 cut(s) 90, 540
FaeI CATG 5 cut(s) 94, 208, 221, 415, 463
FaiI YATR 9 cut(s) 92, 206, 219, 377, 409, 413, 461, 469, 471
FaqI GGGAC 1 cut(s) 316
FatI CATG 5 cut(s) 90, 204, 217, 411, 459
FauNDI CATATG 1 cut(s) 469
FbaI TGATCA 1 cut(s) 39
FblI GTMKAC 1 cut(s) 72
Fnu4HI GCNGC 3 cut(s) 185, 447, 498
FokI GGATG 2 cut(s) 131, 244
Fsp4HI GCNGC 3 cut(s) 185, 447, 498
FspBI CTAG 1 cut(s) 431
GluI GCNGC 3 cut(s) 185, 447, 498
HaeIII GGCC 3 cut(s) 68, 89, 110
HgaI GACGC 1 cut(s) 12
Hin1II CATG 5 cut(s) 94, 208, 221, 415, 463
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HindIII AAGCTT 1 cut(s) 311
HinfI GANTC 1 cut(s) 32
HphI GGTGA 3 cut(s) 46, 354, 396
Hpy166II GTNNAC 1 cut(s) 73
Hpy188III TCNNGA 4 cut(s) 128, 218, 224, 272
Hpy8I GTNNAC 1 cut(s) 73
Hpy99I CGWCG 3 cut(s) 28, 74, 134
HpyAV CCTTC 5 cut(s) 79, 193, 316, 414, 430
HpyCH4III ACNGT 1 cut(s) 52
HpyCH4IV ACGT 1 cut(s) 359
HpyCH4V TGCA 3 cut(s) 173, 329, 446
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 86, 184, 470
HpyF3I CTNAG 2 cut(s) 9, 282
HpySE526I ACGT 1 cut(s) 359
Hsp92II CATG 5 cut(s) 94, 208, 221, 415, 463
KpnI GGTACC 1 cut(s) 51
Ksp22I TGATCA 1 cut(s) 39
Kzo9I GATC 5 cut(s) 39, 165, 220, 322, 523
LguI GCTCTTC 1 cut(s) 381
LmnI GCTCC 3 cut(s) 20, 94, 505
Lsp1109I GCAGC 3 cut(s) 196, 458, 484
LweI GCATC 2 cut(s) 160, 182
MabI ACCWGGT 1 cut(s) 120
MaeI CTAG 1 cut(s) 431
MaeII ACGT 1 cut(s) 359
MaeIII GTNAC 4 cut(s) 52, 360, 402, 455
MalI GATC 5 cut(s) 41, 167, 222, 324, 525
MboI GATC 5 cut(s) 39, 165, 220, 322, 523
MboII GAAGA 2 cut(s) 398, 520
MflI RGATCY 1 cut(s) 322
MluCI AATT 4 cut(s) 194, 244, 394, 481
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 1 cut(s) 9
MnlI CCTC 3 cut(s) 149, 313, 410
Mph1103I ATGCAT 1 cut(s) 175
MslI CAYNNNNRTG 2 cut(s) 80, 367
MspR9I CCNGG 2 cut(s) 107, 122
MvaI CCWGG 2 cut(s) 107, 122
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 4 cut(s) 14, 86, 184, 470
NcoI CCATGG 1 cut(s) 90
NdeI CATATG 1 cut(s) 469
NdeII GATC 5 cut(s) 39, 165, 220, 322, 523
NlaIII CATG 5 cut(s) 94, 208, 221, 415, 463
NlaIV GGNNCC 3 cut(s) 49, 304, 324
NmuCI GTSAC 4 cut(s) 52, 360, 402, 455
NruI TCGCGA 1 cut(s) 129
NsiI ATGCAT 1 cut(s) 175
NspI RCATGY 1 cut(s) 208
OliI CACNNNNGTG 2 cut(s) 80, 367
PagI TCATGA 1 cut(s) 217
PciI ACATGT 1 cut(s) 204
PciSI GCTCTTC 1 cut(s) 381
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PkrI GCNGC 3 cut(s) 186, 448, 499
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PpuMI RGGWCCY 2 cut(s) 118, 303
PscI ACATGT 1 cut(s) 204
PshAI GACNNNNGTC 1 cut(s) 68
PsiI TTATAA 1 cut(s) 377
Psp5II RGGWCCY 2 cut(s) 118, 303
Psp6I CCWGG 2 cut(s) 105, 120
PspGI CCWGG 2 cut(s) 105, 120
PspN4I GGNNCC 3 cut(s) 49, 304, 324
PspPI GGNCC 3 cut(s) 103, 118, 303
PspPPI RGGWCCY 2 cut(s) 118, 303
PsuI RGATCY 1 cut(s) 322
RruI TCGCGA 1 cut(s) 129
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
RseI CAYNNNNRTG 2 cut(s) 80, 367
SalI GTCGAC 1 cut(s) 71
SapI GCTCTTC 1 cut(s) 381
SatI GCNGC 3 cut(s) 185, 447, 498
Sau3AI GATC 5 cut(s) 39, 165, 220, 322, 523
Sau96I GGNCC 3 cut(s) 103, 118, 303
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 2 cut(s) 107, 122
SexAI ACCWGGT 1 cut(s) 120
SfaNI GCATC 2 cut(s) 160, 182
SinI GGWCC 3 cut(s) 103, 118, 303
SmiMI CAYNNNNRTG 2 cut(s) 80, 367
Sse9I AATT 4 cut(s) 194, 244, 394, 481
SsiI CCGC 1 cut(s) 78
SspMI CTAG 1 cut(s) 431
StyD4I CCNGG 2 cut(s) 105, 120
StyI CCWWGG 2 cut(s) 90, 540
TaaI ACNGT 1 cut(s) 52
TaiI ACGT 1 cut(s) 362
TaqI TCGA 3 cut(s) 72, 168, 526
TasI AATT 4 cut(s) 194, 244, 394, 481
TscAI CASTG 2 cut(s) 49, 298
TseFI GTSAC 4 cut(s) 52, 360, 402, 455
TseI GCWGC 3 cut(s) 184, 446, 497
Tsp45I GTSAC 4 cut(s) 52, 360, 402, 455
TspRI CASTG 2 cut(s) 49, 298
VpaK11BI GGWCC 3 cut(s) 103, 118, 303
XapI RAATTY 2 cut(s) 244, 394
XceI RCATGY 1 cut(s) 208
XcmI CCANNNNNNNNNTGG 1 cut(s) 118
XmiI GTMKAC 1 cut(s) 72
XspI CTAG 1 cut(s) 431
Zsp2I ATGCAT 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.