Rorug05G0239700

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001324
Physical Location & Seq
Reverse (-)
25398609 .. 25406771
8163 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0239700.1

Sequence Viewer

Length: 1416 bp
ATGGCTGGTGAAATTCTTTGCGCATCACATTTATGTTCCTCAGATGGTGCTAGTTCATCAAAGCCTATATTTGAGGGTGCAAGTGAGACATTTTCAGATGGCCTGTCACCTCAAGTGCAGTGTACAAGCGAAGTTCCTGCAGCACAGTCATCTGACAGCATTCTTCAAAAGACCTCTAATGGAGTTGGCAATAACAGAGCATCAAAGTCAAAAAGACCTCCTCTATCAGTGGGTACCGTTTCTGGTGTGATAGGAAAAGACTACAGTAGGCAGAAGACATCAACAAATGAATCTGCTGAAATGCAGAAACCATTAAAATTAGAATCGGAAGAAATGCCTTTCTCTGATTTATCGAGTGAAGAAGTCCAATCAAGCATGACAGCTAACAACAGTAAGATGCACGCCGATATTGAAGAATTTCTGTTTGAAATGCTTGGAGATGGCTTTAAACTTAATATGAATGTGATCAAAGAAGTTCTCAGCTTTTGTGGATATGATAGGGAAAAGAGCATGGAGAAACTTCTTTTTATGTCAGCATCAACCTTGGAAAATTCTGATGATCTTGTTAGTTTAGAAAGTTCAAAGTCTACAGAGAAGTGTTCAGATCAACATTCTGCTCATAGTAATGGATCTAGAAATGCAAGAAATCCAATAGAAGGTGGGTCTACTGACTCTGCTCTTGAGAAAGAGTTATTTGAAGCATTGATTTGTGTTCCTGAAAGATCGGAAGAAGCACCTAAAGTAAATCTCCCATCAAGGGGACTGAAGAGAAATGGATATAGGGGATTTCATGGTAATTTGGTGATTGAACCTTTTAGAGACACAATTATAGAAAACAAAATTGCGACATTGGTACGACGAGAAGTCACCAGGGCAGAATCTGAGGATGTTGAGAATAGCTATGAGGTTCTACGTAATTCTGTGAAGGAATATTGGATTACAATGAAGGAGTATTACAAAACTGCTTTAGATGCATTTTCTGAGGGTGATCATGTACGTGCAGATAAATTTTTTGAGAAGGGCCATTTCTTTTGCAGAAAGGCACGGGAAGCAGATGACAAATCCACTGAAAAACTTCTTCAGACTAGAGATGATGGTGATTCTGTGTCTCTTGACTTGCATGATCATGAACCCAAGGAAGCTATACGTACTCTAAGGTTTCAATTAACCTATCTCTCTGGCATGCCAGCTTTCAAGTACCTTAGGATCATAGTGGGAAGCAATGATGAAGACACCACTCGAGGAGCGCGGAGAAGACTGATTATAAAGCAACTGGAGAAGGAATCGATCAAATGGACTGAGGAGGAGAATGGGCAGATAATATTGATCCGACTCGATGTGATTGACCCGAAACGCTTGAGCTTCTCAAAAAAATCGAATGAAAGAATCCAGAGCTCACCTTCATTTGGGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

471

Amino Acids

52.73

Weight (kDa)

5.42

Isoelectric Point (pI)

49.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1771 PF08590 301 - 363 1.1e-11 Domain of unknown function (DUF1771)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1265
Acc16I TGCGCA 1 cut(s) 22
Acc65I GGTACC 1 cut(s) 233
AccB1I GGYRCC 1 cut(s) 233
AccI GTMKAC 2 cut(s) 587, 665
AccII CGCG 1 cut(s) 1249
AciI CCGC 1 cut(s) 1249
AclWI GGATC 3 cut(s) 637, 1214, 1321
AcsI RAATTY 4 cut(s) 12, 416, 550, 1007
AcuI CTGAAG 2 cut(s) 785, 1064
AfaI GTAC 6 cut(s) 124, 235, 855, 996, 1150, 1199
AfiI CCNNNNNNNGG 4 cut(s) 656, 757, 758, 1406
AgsI TTSAA 8 cut(s) 167, 413, 428, 582, 698, 809, 1163, 1195
AhdI GACNNNNNGTC 1 cut(s) 863
AjnI CCWGG 1 cut(s) 869
AluBI AGCT 7 cut(s) 383, 483, 900, 1142, 1190, 1362, 1395
AluI AGCT 7 cut(s) 383, 483, 900, 1142, 1190, 1362, 1395
Alw21I GWGCWC 1 cut(s) 1397
Alw26I GTCTC 3 cut(s) 80, 813, 1113
AlwI GGATC 3 cut(s) 637, 1214, 1321
AlwNI CAGNNNCTG 1 cut(s) 881
Ama87I CYCGRG 1 cut(s) 1239
AoxI GGCC 2 cut(s) 100, 1021
ApeKI GCWGC 1 cut(s) 140
ApoI RAATTY 4 cut(s) 12, 416, 550, 1007
Asp700I GAANNNNTTC 2 cut(s) 417, 1074
Asp718I GGTACC 1 cut(s) 233
AspLEI GCGC 2 cut(s) 23, 1249
AspS9I GGNCC 1 cut(s) 1021
AsuHPI GGTGA 7 cut(s) 20, 99, 814, 859, 998, 1109, 1389
AvaI CYCGRG 1 cut(s) 1239
AxyI CCTNAGG 1 cut(s) 1202
BanI GGYRCC 1 cut(s) 233
BanII GRGCYC 1 cut(s) 1397
BarI GAAGNNNNNNTAC 2 cut(s) 938, 970
BbsI GAAGAC 3 cut(s) 281, 1236, 1261
Bbv12I GWGCWC 1 cut(s) 1397
BbvI GCAGC 1 cut(s) 152
BccI CCATC 5 cut(s) 38, 92, 434, 760, 1088
BcgI CGANNNNNNTGC 2 cut(s) 119, 153
BciT130I CCWGG 1 cut(s) 871
BclI TGATCA 3 cut(s) 465, 988, 1123
BcoDI GTCTC 3 cut(s) 80, 813, 1113
BfaI CTAG 3 cut(s) 51, 633, 1086
BfmI CTRYAG 3 cut(s) 138, 262, 588
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
Bme1390I CCNGG 1 cut(s) 871
BmeRI GACNNNNNGTC 1 cut(s) 863
BmeT110I CYCGRG 1 cut(s) 1239
BmgT120I GGNCC 1 cut(s) 1021
BmiI GGNNCC 1 cut(s) 235
BmrFI CCNGG 1 cut(s) 871
BmsI GCATC 5 cut(s) 32, 209, 387, 545, 961
BpiI GAAGAC 3 cut(s) 281, 1236, 1261
BpmI CTGGAG 1 cut(s) 1295
BpuEI CTTGAG 3 cut(s) 96, 701, 1378
Bsa29I ATCGAT 1 cut(s) 1286
BsaAI YACGTR 3 cut(s) 914, 998, 1148
BsaJI CCNNGG 3 cut(s) 543, 870, 1134
Bsc4I CCNNNNNNNGG 4 cut(s) 656, 757, 758, 1406
Bse1I ACTGG 1 cut(s) 1278
Bse21I CCTNAGG 1 cut(s) 1202
Bse3DI GCAATG 1 cut(s) 1228
BseBI CCWGG 1 cut(s) 871
BseCI ATCGAT 1 cut(s) 1286
BseDI CCNNGG 3 cut(s) 543, 870, 1134
BseGI GGATG 1 cut(s) 892
BseLI CCNNNNNNNGG 4 cut(s) 656, 757, 758, 1406
BseMI GCAATG 1 cut(s) 1228
BseMII CTCAG 5 cut(s) 54, 493, 873, 972, 1290
BseNI ACTGG 1 cut(s) 1278
BseRI GAGGAG 4 cut(s) 210, 1257, 1316, 1319
BseXI GCAGC 1 cut(s) 152
BsgI GTGCAG 2 cut(s) 137, 1020
Bsh1236I CGCG 1 cut(s) 1249
BshFI GGCC 2 cut(s) 102, 1023
BshNI GGYRCC 1 cut(s) 233
BshVI ATCGAT 1 cut(s) 1286
BsiHKAI GWGCWC 1 cut(s) 1397
BsiHKCI CYCGRG 1 cut(s) 1239
BslFI GGGAC 1 cut(s) 774
BslI CCNNNNNNNGG 4 cut(s) 656, 757, 758, 1406
BsmAI GTCTC 3 cut(s) 80, 813, 1113
BsmFI GGGAC 1 cut(s) 774
BsmI GAATGC 1 cut(s) 159
BsnI GGCC 2 cut(s) 102, 1023
BsoBI CYCGRG 1 cut(s) 1239
Bsp1286I GDGCHC 1 cut(s) 1397
Bsp1407I TGTACA 1 cut(s) 122
BspACI CCGC 1 cut(s) 1249
BspANI GGCC 2 cut(s) 102, 1023
BspCNI CTCAG 5 cut(s) 53, 492, 874, 973, 1291
BspDI ATCGAT 1 cut(s) 1286
BspFNI CGCG 1 cut(s) 1249
BspHI TCATGA 1 cut(s) 1126
BspLI GGNNCC 1 cut(s) 235
BspMAI CTGCAG 1 cut(s) 142
BspPI GGATC 3 cut(s) 637, 1214, 1321
BspT107I GGYRCC 1 cut(s) 233
BsrDI GCAATG 1 cut(s) 1228
BsrGI TGTACA 1 cut(s) 122
BsrI ACTGG 1 cut(s) 1278
BssECI CCNNGG 3 cut(s) 543, 870, 1134
BssT1I CCWWGG 2 cut(s) 543, 1134
Bst2UI CCWGG 1 cut(s) 871
Bst4CI ACNGT 4 cut(s) 147, 238, 266, 392
Bst6I CTCTTC 1 cut(s) 761
BstAUI TGTACA 1 cut(s) 122
BstBAI YACGTR 3 cut(s) 914, 998, 1148
BstC8I GCNNGC 3 cut(s) 402, 1184, 1188
BstDEI CTNAG 7 cut(s) 40, 479, 882, 981, 1154, 1202, 1299
BstF5I GGATG 1 cut(s) 892
BstFNI CGCG 1 cut(s) 1249
BstHHI GCGC 2 cut(s) 23, 1249
BstMAI GTCTC 3 cut(s) 80, 813, 1113
BstMWI GCNNNNNNNGC 2 cut(s) 971, 1049
BstNI CCWGG 1 cut(s) 871
BstNSI RCATGY 1 cut(s) 1186
BstSCI CCNGG 1 cut(s) 869
BstSFI CTRYAG 3 cut(s) 138, 262, 588
BstSNI TACGTA 2 cut(s) 914, 1148
BstUI CGCG 1 cut(s) 1249
BstV1I GCAGC 1 cut(s) 152
BstV2I GAAGAC 3 cut(s) 281, 1236, 1261
BstX2I RGATCY 1 cut(s) 629
BstYI RGATCY 1 cut(s) 629
Bsu15I ATCGAT 1 cut(s) 1286
Bsu36I CCTNAGG 1 cut(s) 1202
BsuRI GGCC 2 cut(s) 102, 1023
BsuTUI ATCGAT 1 cut(s) 1286
BtsCI GGATG 1 cut(s) 892
BtsI GCAGTG 1 cut(s) 125
BtsIMutI CAGTG 3 cut(s) 125, 234, 1065
Cac8I GCNNGC 3 cut(s) 402, 1184, 1188
CaiI CAGNNNCTG 1 cut(s) 881
CciI TCATGA 1 cut(s) 1126
CfoI GCGC 2 cut(s) 23, 1249
Cfr13I GGNCC 1 cut(s) 1021
ClaI ATCGAT 1 cut(s) 1286
Csp6I GTAC 6 cut(s) 123, 234, 854, 995, 1149, 1198
CviAII CATG 7 cut(s) 376, 511, 791, 992, 1121, 1127, 1183
CviQI GTAC 6 cut(s) 123, 234, 854, 995, 1149, 1198
DdeI CTNAG 7 cut(s) 40, 479, 882, 981, 1154, 1202, 1299
DraI TTTAAA 1 cut(s) 448
DriI GACNNNNNGTC 1 cut(s) 863
Eam1104I CTCTTC 1 cut(s) 761
Eam1105I GACNNNNNGTC 1 cut(s) 863
EarI CTCTTC 1 cut(s) 761
Ecl136II GAGCTC 1 cut(s) 1395
Eco105I TACGTA 2 cut(s) 914, 1148
Eco130I CCWWGG 2 cut(s) 543, 1134
Eco24I GRGCYC 1 cut(s) 1397
Eco53kI GAGCTC 1 cut(s) 1395
Eco57I CTGAAG 2 cut(s) 785, 1064
Eco81I CCTNAGG 1 cut(s) 1202
Eco88I CYCGRG 1 cut(s) 1239
EcoICRI GAGCTC 1 cut(s) 1395
EcoRII CCWGG 1 cut(s) 869
EcoT14I CCWWGG 2 cut(s) 543, 1134
EcoT22I ATGCAT 1 cut(s) 976
EcoT38I GRGCYC 1 cut(s) 1397
ErhI CCWWGG 2 cut(s) 543, 1134
FaeI CATG 7 cut(s) 379, 514, 794, 995, 1124, 1130, 1186
FaqI GGGAC 1 cut(s) 774
FatI CATG 7 cut(s) 375, 510, 790, 991, 1120, 1126, 1182
FbaI TGATCA 3 cut(s) 465, 988, 1123
FblI GTMKAC 2 cut(s) 587, 665
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 1 cut(s) 899
FriOI GRGCYC 1 cut(s) 1397
Fsp4HI GCNGC 1 cut(s) 141
FspBI CTAG 3 cut(s) 51, 633, 1086
FspI TGCGCA 1 cut(s) 22
GlaI GCGC 2 cut(s) 22, 1248
GluI GCNGC 1 cut(s) 141
GsuI CTGGAG 1 cut(s) 1295
HaeIII GGCC 2 cut(s) 102, 1023
HhaI GCGC 2 cut(s) 23, 1249
Hin1II CATG 7 cut(s) 379, 514, 794, 995, 1124, 1130, 1186
Hin6I GCGC 2 cut(s) 21, 1247
HinP1I GCGC 2 cut(s) 21, 1247
HinfI GANTC 8 cut(s) 290, 323, 671, 878, 1100, 1283, 1332, 1386
HphI GGTGA 7 cut(s) 20, 99, 814, 859, 998, 1109, 1389
Hpy166II GTNNAC 3 cut(s) 123, 588, 666
Hpy188III TCNNGA 6 cut(s) 633, 680, 716, 1112, 1127, 1390
Hpy8I GTNNAC 3 cut(s) 123, 588, 666
Hpy99I CGWCG 1 cut(s) 861
HpyAV CCTTC 6 cut(s) 650, 919, 940, 1012, 1273, 1410
HpyCH4III ACNGT 4 cut(s) 147, 238, 266, 392
HpyCH4IV ACGT 3 cut(s) 913, 997, 1147
HpyF10VI GCNNNNNNNGC 2 cut(s) 971, 1049
HpyF3I CTNAG 7 cut(s) 40, 479, 882, 981, 1154, 1202, 1299
HpySE526I ACGT 3 cut(s) 913, 997, 1147
Hsp92II CATG 7 cut(s) 379, 514, 794, 995, 1124, 1130, 1186
HspAI GCGC 2 cut(s) 21, 1247
KpnI GGTACC 1 cut(s) 237
Ksp22I TGATCA 3 cut(s) 465, 988, 1123
LmnI GCTCC 1 cut(s) 1244
Lsp1109I GCAGC 1 cut(s) 152
LweI GCATC 5 cut(s) 32, 209, 387, 545, 961
MaeI CTAG 3 cut(s) 51, 633, 1086
MaeII ACGT 3 cut(s) 913, 997, 1147
MaeIII GTNAC 2 cut(s) 105, 865
MflI RGATCY 1 cut(s) 629
MhlI GDGCHC 1 cut(s) 1397
MlyI GAGTC 2 cut(s) 665, 1326
MmeI TCCRAC 1 cut(s) 1354
Mph1103I ATGCAT 1 cut(s) 976
MroXI GAANNNNTTC 2 cut(s) 417, 1074
MseI TTAA 4 cut(s) 314, 447, 453, 1166
MslI CAYNNNNRTG 4 cut(s) 31, 624, 996, 1125
MspR9I CCNGG 1 cut(s) 871
Mva1269I GAATGC 1 cut(s) 159
MvaI CCWGG 1 cut(s) 871
MvnI CGCG 1 cut(s) 1249
MwoI GCNNNNNNNGC 2 cut(s) 971, 1049
NlaIII CATG 7 cut(s) 379, 514, 794, 995, 1124, 1130, 1186
NlaIV GGNNCC 1 cut(s) 235
NmuCI GTSAC 2 cut(s) 105, 865
NsbI TGCGCA 1 cut(s) 22
NsiI ATGCAT 1 cut(s) 976
NspI RCATGY 1 cut(s) 1186
PaeI GCATGC 1 cut(s) 1186
PaeR7I CTCGAG 1 cut(s) 1239
PagI TCATGA 1 cut(s) 1126
PctI GAATGC 1 cut(s) 159
PdmI GAANNNNTTC 2 cut(s) 417, 1074
PfeI GAWTC 6 cut(s) 290, 323, 878, 1100, 1283, 1386
PkrI GCNGC 1 cut(s) 142
PleI GAGTC 2 cut(s) 665, 1326
PpsI GAGTC 2 cut(s) 665, 1326
Ppu21I YACGTR 3 cut(s) 914, 998, 1148
PsiI TTATAA 1 cut(s) 1265
Psp124BI GAGCTC 1 cut(s) 1397
Psp6I CCWGG 1 cut(s) 869
PspGI CCWGG 1 cut(s) 869
PspN4I GGNNCC 1 cut(s) 235
PspPI GGNCC 1 cut(s) 1021
PspXI VCTCGAGB 1 cut(s) 1239
PstI CTGCAG 1 cut(s) 142
PstNI CAGNNNCTG 1 cut(s) 881
PsuI RGATCY 1 cut(s) 629
RsaI GTAC 6 cut(s) 124, 235, 855, 996, 1150, 1199
RsaNI GTAC 6 cut(s) 123, 234, 854, 995, 1149, 1198
RseI CAYNNNNRTG 4 cut(s) 31, 624, 996, 1125
SacI GAGCTC 1 cut(s) 1397
SaqAI TTAA 4 cut(s) 314, 447, 453, 1166
SatI GCNGC 1 cut(s) 141
Sau96I GGNCC 1 cut(s) 1021
SchI GAGTC 2 cut(s) 665, 1326
ScrFI CCNGG 1 cut(s) 871
SduI GDGCHC 1 cut(s) 1397
SfaNI GCATC 5 cut(s) 32, 209, 387, 545, 961
SfcI CTRYAG 3 cut(s) 138, 262, 588
Sfr274I CTCGAG 1 cut(s) 1239
SlaI CTCGAG 1 cut(s) 1239
SmiMI CAYNNNNRTG 4 cut(s) 31, 624, 996, 1125
SmlI CTYRAG 4 cut(s) 111, 680, 1239, 1357
SmoI CTYRAG 4 cut(s) 111, 680, 1239, 1357
SnaBI TACGTA 2 cut(s) 914, 1148
SphI GCATGC 1 cut(s) 1186
SsiI CCGC 1 cut(s) 1249
SspI AATATT 2 cut(s) 932, 1323
SspMI CTAG 3 cut(s) 51, 633, 1086
SstI GAGCTC 1 cut(s) 1397
StyD4I CCNGG 1 cut(s) 869
StyI CCWWGG 2 cut(s) 543, 1134
TaaI ACNGT 4 cut(s) 147, 238, 266, 392
TaiI ACGT 3 cut(s) 916, 1000, 1150
TaqI TCGA 5 cut(s) 353, 1240, 1286, 1335, 1376
TatI WGTACW 1 cut(s) 122
TfiI GAWTC 6 cut(s) 290, 323, 878, 1100, 1283, 1386
Tru1I TTAA 4 cut(s) 314, 447, 453, 1166
Tru9I TTAA 4 cut(s) 314, 447, 453, 1166
TscAI CASTG 3 cut(s) 125, 234, 1072
TseFI GTSAC 2 cut(s) 105, 865
TseI GCWGC 1 cut(s) 140
Tsp45I GTSAC 2 cut(s) 105, 865
TspDTI ATGAA 9 cut(s) 45, 303, 473, 779, 959, 1143, 1242, 1392, 1395
TspRI CASTG 3 cut(s) 125, 234, 1072
XapI RAATTY 4 cut(s) 12, 416, 550, 1007
XbaI TCTAGA 1 cut(s) 632
XceI RCATGY 1 cut(s) 1186
XhoI CTCGAG 1 cut(s) 1239
XmiI GTMKAC 2 cut(s) 587, 665
XmnI GAANNNNTTC 2 cut(s) 417, 1074
XspI CTAG 3 cut(s) 51, 633, 1086
Zsp2I ATGCAT 1 cut(s) 976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.