RLG00000028597

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001324
Physical Location & Seq
Reverse (-)
24865602 .. 24870019
4418 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028597

Sequence Viewer

Length: 921 bp
ATGGAGGCTGAGAAAGCAGGGGCATCGTTGGACTCGCTGATCGCCGGGTTCAACAGTCGGATCTCGGAGCTCCAAGAGCTCGTTATTGCCCGAAACATGTACCCGGCCAGCAGCGTCACCGATTTGACGGCCGTCGACGCCGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGTCATCAAGGACCGGGTTCGCGACGAAACCCTAGCCATCCCCAAAGCGAAAAAACTGATCGATGCATCGCTGAAGCAGCAAAGGAAATTGAAGGATATGTCAGTTTATGCCCCCACTCATGTTCCGGAAAGTATGTCGATGTTGAATTTGGATACCAACAGATGTTTATTTCCAGAAAGCTCTCAGCAAAACACTGGTTCTGGGACCTTCAAGCTTGAGGAGGATCCTGCACCGCCGCCACCACCTAAGGTGCATCTCTCTCTCTCTCTCTCTCACACACACACACACACACACACTTGTCAATTTATGAGACATCTATTCTTTCTGTTTTTGTTGATTTCAGGAGAAAAAGGGTGTTCAATTCAACCATTGTGGTTTGTAACTGCTGAGGAGCTGGATTCCTTGTCATCATACATGAGAGGAAGGCTTACACTAGAGAAGGTCAATGCAGCTATTAATGACCTGGTGACATATGCTGAAGCAAATTATCAGCTCATAGCTGCTCCAAAGAAGAATGTGGCAGGAGATCGTTGGGAAAAGGCTTTGGTTGAGGCCGGGATCGGAATCGGGACCATCAAGCAGTGGCTGACGATTGAGGGCGAGGGACTTTCATCGAATCGCAGCGGCCCGTGCGGACGGATGGAGCTCTTCTTCCTCTCTCTGGCTCTCTCTCTCTTCTTTCTATTATGCTTCTGTCACACTCGATCTCTCTATCTCTCTCTGGATCTCGTTCTCTATCTGCAGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

307

Amino Acids

33.98

Weight (kDa)

6.25

Isoelectric Point (pI)

40.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 9 - 238 1.8e-49 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 135
AccII CGCG 1 cut(s) 192
AccIII TCCGGA 1 cut(s) 295
AciI CCGC 5 cut(s) 141, 404, 407, 795, 804
AclWI GGATC 5 cut(s) 68, 389, 402, 737, 903
AcoI YGGCCR 2 cut(s) 105, 129
AcsI RAATTY 1 cut(s) 316
AcuI CTGAAG 3 cut(s) 146, 263, 669
AcyI GRCGYC 1 cut(s) 138
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 1 cut(s) 832
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 6 cut(s) 52, 262, 316, 382, 531, 536
AjnI CCWGG 2 cut(s) 168, 633
Alw21I GWGCWC 3 cut(s) 72, 81, 819
Alw26I GTCTC 1 cut(s) 475
AlwI GGATC 5 cut(s) 68, 389, 402, 737, 903
AlwNI CAGNNNCTG 1 cut(s) 757
Aor13HI TCCGGA 1 cut(s) 295
AoxI GGCC 5 cut(s) 105, 129, 150, 723, 796
ApeKI GCWGC 5 cut(s) 111, 247, 620, 671, 792
ApoI RAATTY 1 cut(s) 316
AseI ATTAAT 1 cut(s) 627
AspS9I GGNCC 5 cut(s) 166, 181, 375, 741, 797
AsuC2I CCSGG 4 cut(s) 46, 104, 185, 727
AsuHPI GGTGA 2 cut(s) 109, 649
AvaII GGWCC 4 cut(s) 166, 181, 375, 741
AxyI CCTNAGG 1 cut(s) 417
BamHI GGATCC 1 cut(s) 394
BanII GRGCYC 3 cut(s) 72, 81, 819
Bbv12I GWGCWC 3 cut(s) 72, 81, 819
BbvCI CCTCAGC 1 cut(s) 558
BbvI GCAGC 5 cut(s) 123, 259, 632, 658, 804
BccI CCATC 3 cut(s) 215, 752, 805
BceAI ACGGC 3 cut(s) 116, 128, 144
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BciT130I CCWGG 2 cut(s) 170, 635
BciVI GTATCC 1 cut(s) 316
BcnI CCSGG 4 cut(s) 46, 104, 185, 727
BcoDI GTCTC 1 cut(s) 475
BfaI CTAG 3 cut(s) 203, 605, 919
BfmI CTRYAG 1 cut(s) 911
BfuI GTATCC 1 cut(s) 316
BglI GCCNNNNNGGC 1 cut(s) 149
BglII AGATCT 1 cut(s) 915
BisI GCNGC 8 cut(s) 112, 141, 248, 407, 621, 672, 793, 796
BlsI GCNGC 8 cut(s) 113, 142, 249, 408, 622, 673, 794, 797
Bme1390I CCNGG 6 cut(s) 46, 104, 170, 185, 635, 727
Bme18I GGWCC 4 cut(s) 166, 181, 375, 741
BmgT120I GGNCC 5 cut(s) 166, 181, 375, 741, 797
BmiI GGNNCC 3 cut(s) 376, 396, 742
BmrFI CCNGG 6 cut(s) 46, 104, 170, 185, 635, 727
BmsI GCATC 4 cut(s) 32, 223, 245, 433
BoxI GACNNNNGTC 1 cut(s) 131
Bpu10I CCTNAGC 1 cut(s) 558
BpuEI CTTGAG 1 cut(s) 407
BpuMI CCSGG 4 cut(s) 46, 104, 185, 727
Bsa29I ATCGAT 1 cut(s) 231
BsaBI GATNNNNATC 1 cut(s) 734
BsaHI GRCGYC 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 153
BsaWI WCCGGW 1 cut(s) 295
Bsc4I CCNNNNNNNGG 1 cut(s) 832
Bse1I ACTGG 1 cut(s) 370
Bse21I CCTNAGG 1 cut(s) 417
Bse8I GATNNNNATC 1 cut(s) 734
BseAI TCCGGA 1 cut(s) 295
BseBI CCWGG 2 cut(s) 170, 635
BseCI ATCGAT 1 cut(s) 231
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 2 cut(s) 207, 816
BseJI GATNNNNATC 1 cut(s) 734
BseLI CCNNNNNNNGG 1 cut(s) 832
BseMII CTCAG 2 cut(s) 368, 549
BseNI ACTGG 1 cut(s) 370
BseRI GAGGAG 2 cut(s) 404, 575
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 5 cut(s) 123, 259, 632, 658, 804
BsgI GTGCAG 1 cut(s) 384
Bsh1236I CGCG 1 cut(s) 192
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 5 cut(s) 107, 131, 152, 725, 798
BshVI ATCGAT 1 cut(s) 231
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 3 cut(s) 72, 81, 819
BsiSI CCGG 5 cut(s) 45, 104, 184, 296, 726
BslFI GGGAC 3 cut(s) 388, 754, 789
BslI CCNNNNNNNGG 1 cut(s) 832
BsmAI GTCTC 1 cut(s) 475
BsmFI GGGAC 3 cut(s) 388, 754, 789
BsnI GGCC 5 cut(s) 107, 131, 152, 725, 798
Bsp1286I GDGCHC 3 cut(s) 72, 81, 819
Bsp13I TCCGGA 1 cut(s) 295
Bsp143I GATC 9 cut(s) 39, 60, 228, 394, 697, 729, 875, 895, 915
Bsp19I CCATGG 1 cut(s) 153
Bsp68I TCGCGA 1 cut(s) 192
BspACI CCGC 5 cut(s) 141, 404, 407, 795, 804
BspANI GGCC 5 cut(s) 107, 131, 152, 725, 798
BspCNI CTCAG 2 cut(s) 367, 550
BspDI ATCGAT 1 cut(s) 231
BspEI TCCGGA 1 cut(s) 295
BspFNI CGCG 1 cut(s) 192
BspLI GGNNCC 3 cut(s) 376, 396, 742
BspMAI CTGCAG 1 cut(s) 915
BspPI GGATC 5 cut(s) 68, 389, 402, 737, 903
BspQI GCTCTTC 1 cut(s) 824
BsrI ACTGG 1 cut(s) 370
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 9 cut(s) 39, 60, 228, 394, 697, 729, 875, 895, 915
BssNI GRCGYC 1 cut(s) 138
BssT1I CCWWGG 1 cut(s) 153
Bst2UI CCWGG 2 cut(s) 170, 635
Bst4CI ACNGT 1 cut(s) 56
Bst6I CTCTTC 2 cut(s) 824, 851
BstACI GRCGYC 1 cut(s) 138
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 4 cut(s) 9, 354, 417, 558
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 2 cut(s) 207, 816
BstFNI CGCG 1 cut(s) 192
BstKTI GATC 9 cut(s) 42, 63, 231, 397, 700, 732, 878, 898, 918
BstMAI GTCTC 1 cut(s) 475
BstMBI GATC 9 cut(s) 39, 60, 228, 394, 697, 729, 875, 895, 915
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 6 cut(s) 14, 76, 137, 149, 247, 801
BstNI CCWGG 2 cut(s) 170, 635
BstNSI RCATGY 1 cut(s) 100
BstPAI GACNNNNGTC 1 cut(s) 131
BstSCI CCNGG 6 cut(s) 44, 102, 168, 183, 633, 725
BstSFI CTRYAG 1 cut(s) 911
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 5 cut(s) 123, 259, 632, 658, 804
BstX2I RGATCY 4 cut(s) 60, 394, 895, 915
BstYI RGATCY 4 cut(s) 60, 394, 895, 915
BstZI CGGCCG 1 cut(s) 129
Bsu15I ATCGAT 1 cut(s) 231
Bsu36I CCTNAGG 1 cut(s) 417
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 5 cut(s) 107, 131, 152, 725, 798
BsuTUI ATCGAT 1 cut(s) 231
BtgI CCRYGG 1 cut(s) 153
BtgZI GCGATG 1 cut(s) 222
BtsCI GGATG 2 cut(s) 207, 816
BtsI GCAGTG 1 cut(s) 758
BtsIMutI CAGTG 2 cut(s) 363, 758
BtuMI TCGCGA 1 cut(s) 192
Cac8I GCNNGC 1 cut(s) 109
CaiI CAGNNNCTG 1 cut(s) 757
Cfr13I GGNCC 5 cut(s) 166, 181, 375, 741, 797
ClaI ATCGAT 1 cut(s) 231
CseI GACGC 2 cut(s) 103, 146
CsiI ACCWGGT 1 cut(s) 633
Csp6I GTAC 1 cut(s) 100
CspCI CAANNNNNGTGG 2 cut(s) 524, 559
CviAII CATG 4 cut(s) 97, 154, 290, 586
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 4 cut(s) 9, 354, 417, 558
DpnI GATC 9 cut(s) 41, 62, 230, 396, 699, 731, 877, 897, 917
DpnII GATC 9 cut(s) 39, 60, 228, 394, 697, 729, 875, 895, 915
EaeI YGGCCR 2 cut(s) 105, 129
EagI CGGCCG 1 cut(s) 129
Eam1104I CTCTTC 2 cut(s) 824, 851
EarI CTCTTC 2 cut(s) 824, 851
Ecl136II GAGCTC 3 cut(s) 70, 79, 817
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 153
Eco24I GRGCYC 3 cut(s) 72, 81, 819
Eco47I GGWCC 4 cut(s) 166, 181, 375, 741
Eco52I CGGCCG 1 cut(s) 129
Eco53kI GAGCTC 3 cut(s) 70, 79, 817
Eco57I CTGAAG 3 cut(s) 146, 263, 669
Eco81I CCTNAGG 1 cut(s) 417
EcoICRI GAGCTC 3 cut(s) 70, 79, 817
EcoO109I RGGNCCY 1 cut(s) 375
EcoRII CCWGG 2 cut(s) 168, 633
EcoT14I CCWWGG 1 cut(s) 153
EcoT22I ATGCAT 1 cut(s) 238
EcoT38I GRGCYC 3 cut(s) 72, 81, 819
ErhI CCWWGG 1 cut(s) 153
FaeI CATG 4 cut(s) 100, 157, 293, 589
FaqI GGGAC 3 cut(s) 388, 754, 789
FatI CATG 4 cut(s) 96, 153, 289, 585
FauNDI CATATG 1 cut(s) 643
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 8 cut(s) 112, 141, 248, 407, 621, 672, 793, 796
FokI GGATG 2 cut(s) 194, 823
FriOI GRGCYC 3 cut(s) 72, 81, 819
Fsp4HI GCNGC 8 cut(s) 112, 141, 248, 407, 621, 672, 793, 796
FspBI CTAG 3 cut(s) 203, 605, 919
GluI GCNGC 8 cut(s) 112, 141, 248, 407, 621, 672, 793, 796
HaeIII GGCC 5 cut(s) 107, 131, 152, 725, 798
HapII CCGG 5 cut(s) 45, 104, 184, 296, 726
HgaI GACGC 2 cut(s) 103, 146
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 4 cut(s) 100, 157, 293, 589
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 4 cut(s) 32, 569, 735, 787
HpaII CCGG 5 cut(s) 45, 104, 184, 296, 726
HphI GGTGA 2 cut(s) 109, 649
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 3 cut(s) 60, 67, 734
Hpy188III TCNNGA 6 cut(s) 191, 296, 344, 513, 739, 893
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 3 cut(s) 137, 140, 197
HpyAV CCTTC 5 cut(s) 142, 256, 388, 588, 604
HpyCH4III ACNGT 1 cut(s) 56
HpyCH4V TGCA 5 cut(s) 236, 401, 424, 620, 913
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 76, 137, 149, 247, 801
HpyF3I CTNAG 4 cut(s) 9, 354, 417, 558
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 4 cut(s) 100, 157, 293, 589
Kpn2I TCCGGA 1 cut(s) 295
Kzo9I GATC 9 cut(s) 39, 60, 228, 394, 697, 729, 875, 895, 915
LguI GCTCTTC 1 cut(s) 824
LmnI GCTCC 6 cut(s) 67, 75, 157, 562, 679, 814
Lsp1109I GCAGC 5 cut(s) 123, 259, 632, 658, 804
LweI GCATC 4 cut(s) 32, 223, 245, 433
MabI ACCWGGT 1 cut(s) 633
MaeI CTAG 3 cut(s) 203, 605, 919
MaeIII GTNAC 4 cut(s) 115, 550, 637, 866
MalI GATC 9 cut(s) 41, 62, 230, 396, 699, 731, 877, 897, 917
MboI GATC 9 cut(s) 39, 60, 228, 394, 697, 729, 875, 895, 915
MboII GAAGA 4 cut(s) 694, 811, 814, 838
MflI RGATCY 4 cut(s) 60, 394, 895, 915
MhlI GDGCHC 3 cut(s) 72, 81, 819
MluCI AATT 5 cut(s) 257, 316, 473, 531, 655
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 2 cut(s) 9, 38
MnlI CCTC 8 cut(s) 382, 385, 553, 584, 715, 760, 766, 836
Mph1103I ATGCAT 1 cut(s) 238
MroI TCCGGA 1 cut(s) 295
MseI TTAA 1 cut(s) 627
MspA1I CMGCKG 1 cut(s) 795
MspI CCGG 5 cut(s) 45, 104, 184, 296, 726
MspR9I CCNGG 6 cut(s) 46, 104, 170, 185, 635, 727
MvaI CCWGG 2 cut(s) 170, 635
MvnI CGCG 1 cut(s) 192
MwoI GCNNNNNNNGC 6 cut(s) 14, 76, 137, 149, 247, 801
NciI CCSGG 4 cut(s) 46, 104, 185, 727
NcoI CCATGG 1 cut(s) 153
NdeI CATATG 1 cut(s) 643
NdeII GATC 9 cut(s) 39, 60, 228, 394, 697, 729, 875, 895, 915
NlaIII CATG 4 cut(s) 100, 157, 293, 589
NlaIV GGNNCC 3 cut(s) 376, 396, 742
NmuCI GTSAC 3 cut(s) 115, 637, 866
NruI TCGCGA 1 cut(s) 192
NsiI ATGCAT 1 cut(s) 238
NspI RCATGY 1 cut(s) 100
PciI ACATGT 1 cut(s) 96
PciSI GCTCTTC 1 cut(s) 824
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PfeI GAWTC 3 cut(s) 569, 735, 787
PkrI GCNGC 8 cut(s) 113, 142, 249, 408, 622, 673, 794, 797
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PpuMI RGGWCCY 1 cut(s) 375
PscI ACATGT 1 cut(s) 96
PshAI GACNNNNGTC 1 cut(s) 131
PshBI ATTAAT 1 cut(s) 627
Psp124BI GAGCTC 3 cut(s) 72, 81, 819
Psp5II RGGWCCY 1 cut(s) 375
Psp6I CCWGG 2 cut(s) 168, 633
PspGI CCWGG 2 cut(s) 168, 633
PspN4I GGNNCC 3 cut(s) 376, 396, 742
PspPI GGNCC 5 cut(s) 166, 181, 375, 741, 797
PspPPI RGGWCCY 1 cut(s) 375
PstI CTGCAG 1 cut(s) 915
PstNI CAGNNNCTG 1 cut(s) 757
PsuI RGATCY 4 cut(s) 60, 394, 895, 915
RruI TCGCGA 1 cut(s) 192
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SacI GAGCTC 3 cut(s) 72, 81, 819
SalI GTCGAC 1 cut(s) 134
SapI GCTCTTC 1 cut(s) 824
SaqAI TTAA 1 cut(s) 627
SatI GCNGC 8 cut(s) 112, 141, 248, 407, 621, 672, 793, 796
Sau3AI GATC 9 cut(s) 39, 60, 228, 394, 697, 729, 875, 895, 915
Sau96I GGNCC 5 cut(s) 166, 181, 375, 741, 797
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 6 cut(s) 46, 104, 170, 185, 635, 727
SduI GDGCHC 3 cut(s) 72, 81, 819
SexAI ACCWGGT 1 cut(s) 633
SfaNI GCATC 4 cut(s) 32, 223, 245, 433
SfcI CTRYAG 1 cut(s) 911
SgrDI CGTCGACG 1 cut(s) 134
SinI GGWCC 4 cut(s) 166, 181, 375, 741
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
Sse9I AATT 5 cut(s) 257, 316, 473, 531, 655
SsiI CCGC 5 cut(s) 141, 404, 407, 795, 804
SspMI CTAG 3 cut(s) 203, 605, 919
SstI GAGCTC 3 cut(s) 72, 81, 819
StyD4I CCNGG 6 cut(s) 44, 102, 168, 183, 633, 725
StyI CCWWGG 1 cut(s) 153
TaaI ACNGT 1 cut(s) 56
TaqI TCGA 5 cut(s) 135, 231, 308, 785, 874
TasI AATT 5 cut(s) 257, 316, 473, 531, 655
TauI GCSGC 3 cut(s) 143, 409, 798
TfiI GAWTC 3 cut(s) 569, 735, 787
Tru1I TTAA 1 cut(s) 627
Tru9I TTAA 1 cut(s) 627
TscAI CASTG 2 cut(s) 370, 758
TseFI GTSAC 3 cut(s) 115, 637, 866
TseI GCWGC 5 cut(s) 111, 247, 620, 671, 792
Tsp45I GTSAC 3 cut(s) 115, 637, 866
TspDTI ATGAA 1 cut(s) 771
TspGWI ACGGA 1 cut(s) 823
TspRI CASTG 2 cut(s) 370, 758
VpaK11BI GGWCC 4 cut(s) 166, 181, 375, 741
VspI ATTAAT 1 cut(s) 627
XapI RAATTY 1 cut(s) 316
XceI RCATGY 1 cut(s) 100
XmiI GTMKAC 1 cut(s) 135
XspI CTAG 3 cut(s) 203, 605, 919
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.