Rmu_sc0016526.1_g000001

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0016526.1
OG0001324
Physical Location & Seq
Reverse (-)
913 .. 4069
3157 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0016526.1_g000001.1.cds

Sequence Viewer

Length: 864 bp
atggaggcggagaaagcaggggcgtcgttggactcgctgatcgccgggttcaacagtcggatctcggagctcaaagaactcgttattgcgcgaaacatgtacccagccagcagcgtcaccgatttgtcggccgtcgacgccgccgtcaaggccatggagcttcaggtccaggccatcaaggaccgggttggcgacgaaaccctagccatccccaaagcaaaaaaactgatcgatgcatcgctgaagcagcaaaggaaattgaaggatatgtcagtttatgcccccgctcatgttcctgaaaggatgtcaatgttgaatttggatacaaacagatgtttatttccagaaagctctcagcaaaacactggttctgggaccttcaatcttgacgaggatcctgcaccaccaccaccaaaggagaaaaagggtcgttccacaccaccattgtggtttataagtactgaagagttggattccttgtcatcatacatgagaggaaggcttacactagagaaggtcaatgcagctattagtgacatggcaacatatgctgaagcaaattatcagctcatagctgctccaaagaagaatgtttgtgttctgatttttggtttgtacatctttggcacaggtctgattttggttatcaatgatcaatgggaaaaagccttggaaataagagatatagcaatgcatgaagcagtaaagggaaaacacttctttctcgaaactgacatgaagggaccatccttgaagcttgacaacactggaaaagcaatactaactgtccttcgtcaccttggtcgggtaaatgagacacggattggtcatcatcgggtgatcattcttttgaagcctcactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

287

Amino Acids

31.78

Weight (kDa)

8.38

Isoelectric Point (pI)

37.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 455
AasI GACNNNNNNGTC 1 cut(s) 143
AccBSI CCGCTC 1 cut(s) 287
AccI GTMKAC 1 cut(s) 135
AccII CGCG 1 cut(s) 91
AciI CCGC 3 cut(s) 8, 141, 285
AclWI GGATC 3 cut(s) 68, 389, 402
AcoI YGGCCR 1 cut(s) 129
AcsI RAATTY 1 cut(s) 316
AcuI CTGAAG 4 cut(s) 146, 263, 483, 573
AcyI GRCGYC 2 cut(s) 23, 138
AfaI GTAC 3 cut(s) 101, 460, 617
AfiI CCNNNNNNNGG 1 cut(s) 805
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 6 cut(s) 52, 262, 316, 382, 754, 853
AjnI CCWGG 1 cut(s) 168
AleI CACNNNNGTG 1 cut(s) 445
AluBI AGCT 7 cut(s) 70, 160, 351, 527, 568, 575, 757
AluI AGCT 7 cut(s) 70, 160, 351, 527, 568, 575, 757
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 1 cut(s) 809
AlwI GGATC 3 cut(s) 68, 389, 402
AoxI GGCC 3 cut(s) 129, 150, 171
ApeKI GCWGC 4 cut(s) 111, 247, 524, 575
ApoI RAATTY 1 cut(s) 316
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 4 cut(s) 166, 181, 375, 743
AsuC2I CCSGG 2 cut(s) 46, 185
AsuHPI GGTGA 3 cut(s) 109, 788, 850
AvaII GGWCC 4 cut(s) 166, 181, 375, 743
BamHI GGATCC 1 cut(s) 394
BanII GRGCYC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 72
BbvI GCAGC 4 cut(s) 123, 259, 536, 562
BccI CCATC 3 cut(s) 182, 215, 754
BceAI ACGGC 2 cut(s) 116, 128
BcgI CGANNNNNNTGC 4 cut(s) 6, 40, 380, 414
BciT130I CCWGG 1 cut(s) 170
BciVI GTATCC 1 cut(s) 316
BclI TGATCA 2 cut(s) 652, 840
BcnI CCSGG 2 cut(s) 46, 185
BcoDI GTCTC 1 cut(s) 809
BfaI CTAG 2 cut(s) 203, 509
BfuI GTATCC 1 cut(s) 316
BglI GCCNNNNNGGC 1 cut(s) 149
BisI GCNGC 5 cut(s) 112, 141, 248, 525, 576
BlsI GCNGC 5 cut(s) 113, 142, 249, 526, 577
BmcAI AGTACT 1 cut(s) 460
Bme1390I CCNGG 3 cut(s) 46, 170, 185
Bme18I GGWCC 4 cut(s) 166, 181, 375, 743
BmgT120I GGNCC 4 cut(s) 166, 181, 375, 743
BmiI GGNNCC 3 cut(s) 376, 396, 744
BmrFI CCNGG 3 cut(s) 46, 170, 185
BmsI GCATC 2 cut(s) 223, 245
BpuMI CCSGG 2 cut(s) 46, 185
Bsa29I ATCGAT 1 cut(s) 231
BsaHI GRCGYC 2 cut(s) 23, 138
BsaJI CCNNGG 3 cut(s) 153, 669, 799
Bsc4I CCNNNNNNNGG 1 cut(s) 805
Bse1I ACTGG 2 cut(s) 370, 772
Bse3DI GCAATG 1 cut(s) 696
BseBI CCWGG 1 cut(s) 170
BseCI ATCGAT 1 cut(s) 231
BseDI CCNNGG 3 cut(s) 153, 669, 799
BseGI GGATG 3 cut(s) 207, 309, 746
BseLI CCNNNNNNNGG 1 cut(s) 805
BseMI GCAATG 1 cut(s) 696
BseMII CTCAG 1 cut(s) 368
BseNI ACTGG 2 cut(s) 370, 772
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 4 cut(s) 123, 259, 536, 562
BseYI CCCAGC 1 cut(s) 103
BsgI GTGCAG 1 cut(s) 384
Bsh1236I CGCG 1 cut(s) 91
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 3 cut(s) 131, 152, 173
BshVI ATCGAT 1 cut(s) 231
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 72
BsiSI CCGG 2 cut(s) 45, 184
BslFI GGGAC 2 cut(s) 388, 756
BslI CCNNNNNNNGG 1 cut(s) 805
BsmAI GTCTC 1 cut(s) 809
BsmFI GGGAC 2 cut(s) 388, 756
BsnI GGCC 3 cut(s) 131, 152, 173
Bsp1286I GDGCHC 1 cut(s) 72
Bsp1407I TGTACA 1 cut(s) 615
Bsp143I GATC 6 cut(s) 39, 60, 228, 394, 652, 840
Bsp19I CCATGG 1 cut(s) 153
BspACI CCGC 3 cut(s) 8, 141, 285
BspANI GGCC 3 cut(s) 131, 152, 173
BspCNI CTCAG 1 cut(s) 367
BspDI ATCGAT 1 cut(s) 231
BspFNI CGCG 1 cut(s) 91
BspLI GGNNCC 3 cut(s) 376, 396, 744
BspPI GGATC 3 cut(s) 68, 389, 402
BsrBI CCGCTC 1 cut(s) 287
BsrDI GCAATG 1 cut(s) 696
BsrGI TGTACA 1 cut(s) 615
BsrI ACTGG 2 cut(s) 370, 772
BssECI CCNNGG 3 cut(s) 153, 669, 799
BssMI GATC 6 cut(s) 39, 60, 228, 394, 652, 840
BssNI GRCGYC 2 cut(s) 23, 138
BssT1I CCWWGG 3 cut(s) 153, 669, 799
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 2 cut(s) 56, 787
Bst6I CTCTTC 1 cut(s) 459
BstACI GRCGYC 2 cut(s) 23, 138
BstAPI GCANNNNNTGC 1 cut(s) 548
BstAUI TGTACA 1 cut(s) 615
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 1 cut(s) 354
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 3 cut(s) 207, 309, 746
BstFNI CGCG 1 cut(s) 91
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 6 cut(s) 42, 63, 231, 397, 655, 843
BstMAI GTCTC 1 cut(s) 809
BstMBI GATC 6 cut(s) 39, 60, 228, 394, 652, 840
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 5 cut(s) 14, 137, 149, 247, 548
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 100
BstSCI CCNGG 3 cut(s) 44, 168, 183
BstUI CGCG 1 cut(s) 91
BstV1I GCAGC 4 cut(s) 123, 259, 536, 562
BstX2I RGATCY 2 cut(s) 60, 394
BstXI CCANNNNNNTGG 1 cut(s) 447
BstYI RGATCY 2 cut(s) 60, 394
BstZI CGGCCG 1 cut(s) 129
Bsu15I ATCGAT 1 cut(s) 231
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 3 cut(s) 131, 152, 173
BsuTUI ATCGAT 1 cut(s) 231
BtgI CCRYGG 1 cut(s) 153
BtgZI GCGATG 1 cut(s) 222
BtsCI GGATG 3 cut(s) 207, 309, 746
BtsIMutI CAGTG 3 cut(s) 363, 765, 859
Cac8I GCNNGC 1 cut(s) 109
CfoI GCGC 1 cut(s) 91
Cfr13I GGNCC 4 cut(s) 166, 181, 375, 743
ClaI ATCGAT 1 cut(s) 231
CseI GACGC 3 cut(s) 12, 103, 146
Csp6I GTAC 3 cut(s) 100, 459, 616
CviAII CATG 7 cut(s) 97, 154, 290, 490, 538, 695, 736
CviQI GTAC 3 cut(s) 100, 459, 616
DdeI CTNAG 1 cut(s) 354
DpnI GATC 6 cut(s) 41, 62, 230, 396, 654, 842
DpnII GATC 6 cut(s) 39, 60, 228, 394, 652, 840
DrdI GACNNNNNNGTC 1 cut(s) 143
DseDI GACNNNNNNGTC 1 cut(s) 143
EaeI YGGCCR 1 cut(s) 129
EagI CGGCCG 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 459
EarI CTCTTC 1 cut(s) 459
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 1 cut(s) 70
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 3 cut(s) 153, 669, 799
Eco24I GRGCYC 1 cut(s) 72
Eco47I GGWCC 4 cut(s) 166, 181, 375, 743
Eco52I CGGCCG 1 cut(s) 129
Eco53kI GAGCTC 1 cut(s) 70
Eco57I CTGAAG 4 cut(s) 146, 263, 483, 573
EcoICRI GAGCTC 1 cut(s) 70
EcoO109I RGGNCCY 1 cut(s) 375
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 3 cut(s) 153, 669, 799
EcoT22I ATGCAT 2 cut(s) 238, 696
EcoT38I GRGCYC 1 cut(s) 72
ErhI CCWWGG 3 cut(s) 153, 669, 799
FaeI CATG 7 cut(s) 100, 157, 293, 493, 541, 698, 739
FaqI GGGAC 2 cut(s) 388, 756
FatI CATG 7 cut(s) 96, 153, 289, 489, 537, 694, 735
FauI CCCGC 1 cut(s) 292
FauNDI CATATG 1 cut(s) 547
FbaI TGATCA 2 cut(s) 652, 840
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 5 cut(s) 112, 141, 248, 525, 576
FokI GGATG 3 cut(s) 194, 316, 733
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 5 cut(s) 112, 141, 248, 525, 576
FspBI CTAG 2 cut(s) 203, 509
GlaI GCGC 1 cut(s) 90
GluI GCNGC 5 cut(s) 112, 141, 248, 525, 576
GsaI CCCAGC 1 cut(s) 107
HaeIII GGCC 3 cut(s) 131, 152, 173
HapII CCGG 2 cut(s) 45, 184
HgaI GACGC 3 cut(s) 12, 103, 146
HhaI GCGC 1 cut(s) 91
Hin1I GRCGYC 2 cut(s) 23, 138
Hin1II CATG 7 cut(s) 100, 157, 293, 493, 541, 698, 739
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HindIII AAGCTT 1 cut(s) 755
HinfI GANTC 2 cut(s) 32, 473
HpaII CCGG 2 cut(s) 45, 184
HphI GGTGA 3 cut(s) 109, 788, 850
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 4 cut(s) 60, 67, 603, 636
Hpy188III TCNNGA 4 cut(s) 296, 344, 386, 725
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 4 cut(s) 28, 137, 140, 197
HpyAV CCTTC 6 cut(s) 256, 388, 492, 508, 733, 800
HpyCH4III ACNGT 2 cut(s) 56, 787
HpyCH4V TGCA 4 cut(s) 236, 401, 524, 694
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 137, 149, 247, 548
HpyF3I CTNAG 1 cut(s) 354
Hsp92I GRCGYC 2 cut(s) 23, 138
Hsp92II CATG 7 cut(s) 100, 157, 293, 493, 541, 698, 739
HspAI GCGC 1 cut(s) 89
Ksp22I TGATCA 2 cut(s) 652, 840
Kzo9I GATC 6 cut(s) 39, 60, 228, 394, 652, 840
LmnI GCTCC 3 cut(s) 67, 157, 583
Lsp1109I GCAGC 4 cut(s) 123, 259, 536, 562
LweI GCATC 2 cut(s) 223, 245
MaeI CTAG 2 cut(s) 203, 509
MaeIII GTNAC 3 cut(s) 115, 533, 794
MalI GATC 6 cut(s) 41, 62, 230, 396, 654, 842
MbiI CCGCTC 1 cut(s) 287
MboI GATC 6 cut(s) 39, 60, 228, 394, 652, 840
MboII GAAGA 2 cut(s) 476, 598
MflI RGATCY 2 cut(s) 60, 394
MhlI GDGCHC 1 cut(s) 72
MluCI AATT 3 cut(s) 257, 316, 559
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 3 cut(s) 9, 38, 450
MnlI CCTC 2 cut(s) 385, 488
Mph1103I ATGCAT 2 cut(s) 238, 696
MslI CAYNNNNRTG 1 cut(s) 445
MspI CCGG 2 cut(s) 45, 184
MspR9I CCNGG 3 cut(s) 46, 170, 185
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 1 cut(s) 91
MwoI GCNNNNNNNGC 5 cut(s) 14, 137, 149, 247, 548
NciI CCSGG 2 cut(s) 46, 185
NcoI CCATGG 1 cut(s) 153
NdeI CATATG 1 cut(s) 547
NdeII GATC 6 cut(s) 39, 60, 228, 394, 652, 840
NlaIII CATG 7 cut(s) 100, 157, 293, 493, 541, 698, 739
NlaIV GGNNCC 3 cut(s) 376, 396, 744
NmuCI GTSAC 3 cut(s) 115, 533, 794
NsiI ATGCAT 2 cut(s) 238, 696
NspI RCATGY 1 cut(s) 100
OliI CACNNNNGTG 1 cut(s) 445
PciI ACATGT 1 cut(s) 96
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PfeI GAWTC 1 cut(s) 473
PkrI GCNGC 5 cut(s) 113, 142, 249, 526, 577
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PpuMI RGGWCCY 1 cut(s) 375
PscI ACATGT 1 cut(s) 96
PsiI TTATAA 1 cut(s) 455
Psp124BI GAGCTC 1 cut(s) 72
Psp5II RGGWCCY 1 cut(s) 375
Psp6I CCWGG 1 cut(s) 168
PspFI CCCAGC 1 cut(s) 103
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 3 cut(s) 376, 396, 744
PspPI GGNCC 4 cut(s) 166, 181, 375, 743
PspPPI RGGWCCY 1 cut(s) 375
PsuI RGATCY 2 cut(s) 60, 394
RsaI GTAC 3 cut(s) 101, 460, 617
RsaNI GTAC 3 cut(s) 100, 459, 616
RseI CAYNNNNRTG 1 cut(s) 445
SacI GAGCTC 1 cut(s) 72
SalI GTCGAC 1 cut(s) 134
SatI GCNGC 5 cut(s) 112, 141, 248, 525, 576
Sau3AI GATC 6 cut(s) 39, 60, 228, 394, 652, 840
Sau96I GGNCC 4 cut(s) 166, 181, 375, 743
ScaI AGTACT 1 cut(s) 460
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 3 cut(s) 46, 170, 185
SduI GDGCHC 1 cut(s) 72
SfaNI GCATC 2 cut(s) 223, 245
SgrDI CGTCGACG 1 cut(s) 134
SinI GGWCC 4 cut(s) 166, 181, 375, 743
SmiMI CAYNNNNRTG 1 cut(s) 445
Sse9I AATT 3 cut(s) 257, 316, 559
SsiI CCGC 3 cut(s) 8, 141, 285
SspMI CTAG 2 cut(s) 203, 509
SstI GAGCTC 1 cut(s) 72
StyD4I CCNGG 3 cut(s) 44, 168, 183
StyI CCWWGG 3 cut(s) 153, 669, 799
TaaI ACNGT 2 cut(s) 56, 787
TaqI TCGA 3 cut(s) 135, 231, 726
TasI AATT 3 cut(s) 257, 316, 559
TatI WGTACW 2 cut(s) 458, 615
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 1 cut(s) 473
TscAI CASTG 2 cut(s) 370, 772
TseFI GTSAC 3 cut(s) 115, 533, 794
TseI GCWGC 4 cut(s) 111, 247, 524, 575
Tsp45I GTSAC 3 cut(s) 115, 533, 794
TspDTI ATGAA 2 cut(s) 711, 752
TspGWI ACGGA 1 cut(s) 835
TspRI CASTG 2 cut(s) 370, 772
VpaK11BI GGWCC 4 cut(s) 166, 181, 375, 743
XapI RAATTY 1 cut(s) 316
XceI RCATGY 1 cut(s) 100
XmiI GTMKAC 1 cut(s) 135
XspI CTAG 2 cut(s) 203, 509
ZrmI AGTACT 1 cut(s) 460
Zsp2I ATGCAT 2 cut(s) 238, 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.