Rh1CG204600

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0001324
Physical Location & Seq
Forward (+)
44390656 .. 44393125
2470 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG204600.1

Sequence Viewer

Length: 441 bp
ATGGAGGCTGAGCAAGCAGGGGCGTCGTTGGACTCGCTGATCGCCGTGTTCAACAGTCGGATCTCGGAGCTCCAAGAGCTCGTCATTGCGCGAAACATGTACCCAGCCAGCAGCGTCACCGATTTGACGGCCGTCGACGCCGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGCTATCAAGCACCGGGTTCGCGACGAAACCCTAGCCATCCCCAAAGCAAAAGTGGAAGGAGATCGATGGGAAAAAGCCTTGGAAATAAGAGATATAGCAATGCATGAAGCAGTAAAGGGAAAACACTTTCTCGAAACTGACATGAAGGGACCATCCTTGAAGCTTGACAACTCTGGAAAAGCAATACTAACTGTCCTTCGTCACCTTGGTCGTGTAAGTGAGGCACGGATTGGTCATCATCGGGTGATCATTCTTTTGAAGCCTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

146

Amino Acids

16.18

Weight (kDa)

8.07

Isoelectric Point (pI)

33.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 9 - 74 5.9e-15 Spindle and kinetochore-associated protein 1
SKA1 PF07160 68 - 136 5.5e-22 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 135
AccII CGCG 2 cut(s) 91, 192
AciI CCGC 1 cut(s) 141
AclWI GGATC 1 cut(s) 68
AcoI YGGCCR 1 cut(s) 129
AcuI CTGAAG 1 cut(s) 146
AcyI GRCGYC 2 cut(s) 23, 138
AfaI GTAC 1 cut(s) 101
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 3 cut(s) 52, 331, 430
AjnI CCWGG 1 cut(s) 168
AluBI AGCT 4 cut(s) 70, 79, 160, 334
AluI AGCT 4 cut(s) 70, 79, 160, 334
Alw21I GWGCWC 2 cut(s) 72, 81
AlwI GGATC 1 cut(s) 68
AoxI GGCC 2 cut(s) 129, 150
ApeKI GCWGC 1 cut(s) 111
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 2 cut(s) 166, 320
AsuC2I CCSGG 1 cut(s) 185
AsuHPI GGTGA 3 cut(s) 109, 365, 427
AvaII GGWCC 2 cut(s) 166, 320
BanII GRGCYC 2 cut(s) 72, 81
Bbv12I GWGCWC 2 cut(s) 72, 81
BbvI GCAGC 1 cut(s) 123
BccI CCATC 3 cut(s) 215, 231, 331
BceAI ACGGC 4 cut(s) 29, 116, 128, 144
BcgI CGANNNNNNTGC 4 cut(s) 6, 40, 170, 204
BciT130I CCWGG 1 cut(s) 170
BclI TGATCA 1 cut(s) 417
BcnI CCSGG 1 cut(s) 185
BfaI CTAG 1 cut(s) 203
BglI GCCNNNNNGGC 1 cut(s) 149
BisI GCNGC 2 cut(s) 112, 141
BlpI GCTNAGC 1 cut(s) 9
BlsI GCNGC 2 cut(s) 113, 142
Bme1390I CCNGG 2 cut(s) 170, 185
Bme18I GGWCC 2 cut(s) 166, 320
BmgT120I GGNCC 2 cut(s) 166, 320
BmiI GGNNCC 1 cut(s) 321
BmrFI CCNGG 2 cut(s) 170, 185
BoxI GACNNNNGTC 1 cut(s) 131
Bpu1102I GCTNAGC 1 cut(s) 9
BpuMI CCSGG 1 cut(s) 185
Bsa29I ATCGAT 1 cut(s) 235
BsaHI GRCGYC 2 cut(s) 23, 138
BsaJI CCNNGG 3 cut(s) 153, 249, 376
Bse3DI GCAATG 2 cut(s) 84, 276
BseBI CCWGG 1 cut(s) 170
BseCI ATCGAT 1 cut(s) 235
BseDI CCNNGG 3 cut(s) 153, 249, 376
BseGI GGATG 2 cut(s) 207, 323
BseMI GCAATG 2 cut(s) 84, 276
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 1 cut(s) 123
BseYI CCCAGC 1 cut(s) 103
Bsh1236I CGCG 2 cut(s) 91, 192
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 2 cut(s) 131, 152
BshVI ATCGAT 1 cut(s) 235
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 2 cut(s) 72, 81
BsiSI CCGG 1 cut(s) 184
BslFI GGGAC 1 cut(s) 333
BsmFI GGGAC 1 cut(s) 333
BsnI GGCC 2 cut(s) 131, 152
Bsp1286I GDGCHC 2 cut(s) 72, 81
Bsp143I GATC 4 cut(s) 39, 60, 232, 417
Bsp1720I GCTNAGC 1 cut(s) 9
Bsp19I CCATGG 1 cut(s) 153
Bsp68I TCGCGA 1 cut(s) 192
BspACI CCGC 1 cut(s) 141
BspANI GGCC 2 cut(s) 131, 152
BspDI ATCGAT 1 cut(s) 235
BspFNI CGCG 2 cut(s) 91, 192
BspLI GGNNCC 1 cut(s) 321
BspPI GGATC 1 cut(s) 68
BsrDI GCAATG 2 cut(s) 84, 276
BssECI CCNNGG 3 cut(s) 153, 249, 376
BssMI GATC 4 cut(s) 39, 60, 232, 417
BssNI GRCGYC 2 cut(s) 23, 138
BssT1I CCWWGG 3 cut(s) 153, 249, 376
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 2 cut(s) 56, 364
BstACI GRCGYC 2 cut(s) 23, 138
BstC8I GCNNGC 2 cut(s) 15, 109
BstDEI CTNAG 1 cut(s) 9
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 2 cut(s) 207, 323
BstFNI CGCG 2 cut(s) 91, 192
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 4 cut(s) 42, 63, 235, 420
BstMBI GATC 4 cut(s) 39, 60, 232, 417
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 4 cut(s) 14, 76, 137, 149
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 100
BstPAI GACNNNNGTC 1 cut(s) 131
BstSCI CCNGG 2 cut(s) 168, 183
BstUI CGCG 2 cut(s) 91, 192
BstV1I GCAGC 1 cut(s) 123
BstX2I RGATCY 1 cut(s) 60
BstYI RGATCY 1 cut(s) 60
BstZI CGGCCG 1 cut(s) 129
Bsu15I ATCGAT 1 cut(s) 235
BsuRI GGCC 2 cut(s) 131, 152
BsuTUI ATCGAT 1 cut(s) 235
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 2 cut(s) 207, 323
BtsIMutI CAGTG 1 cut(s) 436
BtuMI TCGCGA 1 cut(s) 192
Cac8I GCNNGC 2 cut(s) 15, 109
CfoI GCGC 1 cut(s) 91
Cfr13I GGNCC 2 cut(s) 166, 320
ClaI ATCGAT 1 cut(s) 235
CseI GACGC 3 cut(s) 12, 103, 146
Csp6I GTAC 1 cut(s) 100
CviAII CATG 4 cut(s) 97, 154, 275, 313
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 1 cut(s) 9
DpnI GATC 4 cut(s) 41, 62, 234, 419
DpnII GATC 4 cut(s) 39, 60, 232, 417
EaeI YGGCCR 1 cut(s) 129
EagI CGGCCG 1 cut(s) 129
Ecl136II GAGCTC 2 cut(s) 70, 79
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 3 cut(s) 153, 249, 376
Eco24I GRGCYC 2 cut(s) 72, 81
Eco47I GGWCC 2 cut(s) 166, 320
Eco52I CGGCCG 1 cut(s) 129
Eco53kI GAGCTC 2 cut(s) 70, 79
Eco57I CTGAAG 1 cut(s) 146
EcoICRI GAGCTC 2 cut(s) 70, 79
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 3 cut(s) 153, 249, 376
EcoT22I ATGCAT 1 cut(s) 276
EcoT38I GRGCYC 2 cut(s) 72, 81
ErhI CCWWGG 3 cut(s) 153, 249, 376
FaeI CATG 4 cut(s) 100, 157, 278, 316
FaiI YATR 5 cut(s) 98, 155, 266, 276, 314
FaqI GGGAC 1 cut(s) 333
FatI CATG 4 cut(s) 96, 153, 274, 312
FbaI TGATCA 1 cut(s) 417
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 2 cut(s) 112, 141
FokI GGATG 2 cut(s) 194, 310
FriOI GRGCYC 2 cut(s) 72, 81
Fsp4HI GCNGC 2 cut(s) 112, 141
FspBI CTAG 1 cut(s) 203
GlaI GCGC 1 cut(s) 90
GluI GCNGC 2 cut(s) 112, 141
GsaI CCCAGC 1 cut(s) 107
HaeIII GGCC 2 cut(s) 131, 152
HapII CCGG 1 cut(s) 184
HgaI GACGC 3 cut(s) 12, 103, 146
HhaI GCGC 1 cut(s) 91
Hin1I GRCGYC 2 cut(s) 23, 138
Hin1II CATG 4 cut(s) 100, 157, 278, 316
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HindIII AAGCTT 1 cut(s) 332
HinfI GANTC 1 cut(s) 32
HpaII CCGG 1 cut(s) 184
HphI GGTGA 3 cut(s) 109, 365, 427
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 2 cut(s) 60, 67
Hpy188III TCNNGA 3 cut(s) 191, 302, 345
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 4 cut(s) 28, 137, 140, 197
HpyAV CCTTC 4 cut(s) 142, 221, 310, 377
HpyCH4III ACNGT 2 cut(s) 56, 364
HpyCH4V TGCA 1 cut(s) 274
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 76, 137, 149
HpyF3I CTNAG 1 cut(s) 9
Hsp92I GRCGYC 2 cut(s) 23, 138
Hsp92II CATG 4 cut(s) 100, 157, 278, 316
HspAI GCGC 1 cut(s) 89
Ksp22I TGATCA 1 cut(s) 417
Kzo9I GATC 4 cut(s) 39, 60, 232, 417
LmnI GCTCC 3 cut(s) 67, 75, 157
LpnPI CCDG 8 cut(s) 3, 117, 121, 149, 155, 182, 197, 330
Lsp1109I GCAGC 1 cut(s) 123
MaeI CTAG 1 cut(s) 203
MaeIII GTNAC 2 cut(s) 115, 371
MalI GATC 4 cut(s) 41, 62, 234, 419
MboI GATC 4 cut(s) 39, 60, 232, 417
MflI RGATCY 1 cut(s) 60
MhlI GDGCHC 2 cut(s) 72, 81
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 2 cut(s) 9, 38
MnlI CCTC 1 cut(s) 385
Mph1103I ATGCAT 1 cut(s) 276
MspI CCGG 1 cut(s) 184
MspR9I CCNGG 2 cut(s) 170, 185
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 2 cut(s) 91, 192
MwoI GCNNNNNNNGC 4 cut(s) 14, 76, 137, 149
NciI CCSGG 1 cut(s) 185
NcoI CCATGG 1 cut(s) 153
NdeII GATC 4 cut(s) 39, 60, 232, 417
NlaIII CATG 4 cut(s) 100, 157, 278, 316
NlaIV GGNNCC 1 cut(s) 321
NmuCI GTSAC 2 cut(s) 115, 371
NruI TCGCGA 1 cut(s) 192
NsiI ATGCAT 1 cut(s) 276
NspI RCATGY 1 cut(s) 100
PciI ACATGT 1 cut(s) 96
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PkrI GCNGC 2 cut(s) 113, 142
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PscI ACATGT 1 cut(s) 96
PshAI GACNNNNGTC 1 cut(s) 131
Psp124BI GAGCTC 2 cut(s) 72, 81
Psp6I CCWGG 1 cut(s) 168
PspFI CCCAGC 1 cut(s) 103
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 1 cut(s) 321
PspPI GGNCC 2 cut(s) 166, 320
PsuI RGATCY 1 cut(s) 60
RruI TCGCGA 1 cut(s) 192
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SacI GAGCTC 2 cut(s) 72, 81
SalI GTCGAC 1 cut(s) 134
SatI GCNGC 2 cut(s) 112, 141
Sau3AI GATC 4 cut(s) 39, 60, 232, 417
Sau96I GGNCC 2 cut(s) 166, 320
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 2 cut(s) 170, 185
SduI GDGCHC 2 cut(s) 72, 81
SetI ASST 6 cut(s) 72, 81, 162, 168, 336, 378
SgrDI CGTCGACG 1 cut(s) 134
SinI GGWCC 2 cut(s) 166, 320
SsiI CCGC 1 cut(s) 141
SspMI CTAG 1 cut(s) 203
SstI GAGCTC 2 cut(s) 72, 81
StyD4I CCNGG 2 cut(s) 168, 183
StyI CCWWGG 3 cut(s) 153, 249, 376
TaaI ACNGT 2 cut(s) 56, 364
TaqI TCGA 3 cut(s) 135, 235, 303
TauI GCSGC 1 cut(s) 143
TseFI GTSAC 2 cut(s) 115, 371
TseI GCWGC 1 cut(s) 111
Tsp45I GTSAC 2 cut(s) 115, 371
TspDTI ATGAA 2 cut(s) 291, 329
TspGWI ACGGA 1 cut(s) 412
VpaK11BI GGWCC 2 cut(s) 166, 320
XceI RCATGY 1 cut(s) 100
XcmI CCANNNNNNNNNTGG 1 cut(s) 220
XmiI GTMKAC 1 cut(s) 135
XspI CTAG 1 cut(s) 203
Zsp2I ATGCAT 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.