RLG00000028589

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001324
Physical Location & Seq
Reverse (-)
24712097 .. 24714225
2129 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028589

Sequence Viewer

Length: 720 bp
ATGGAGGCTGAGAAAGCAGGAGCGTCGTTGGACTCGCTGATCACTGGGTTCAACAGTCGGATCTCGGAGCTCCAAGAACTCGTTATTGCTCGAAACAGTCAGACCCGACCCGATTACCCAGCCCTTTTTCAATTCAATTTCGTCTCCAATAACAATACCGTCACCGATTTGACGGCCGTCGACGCCGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGCCATCAAGGACCTGGTTCGCGACGAAACCCTCGCCATCCCCAAAGCAAAAAAACTGATCGATGCATCGCTGAAGCAGCAAAGGAAATTGAAGGACATGTCAATCACTCATGATCCTGAAAGGATGTCAATGTTGAATTTGGATACAAATAGATGTTTATTTCCAGATAGCTCTCAGCAAAACACTGCTTCTGGGACCTTCAAGCTTGATGAGGATCCTGCACCACCACCAGCACCGCCTAAGGTACATCTCTCTCTCTCTCTCTCTCTCACACACACACACACACTCACACTTCTGGCCACTATTCTTTCTGTTTTTGTTAATTCCACGTCACCATTGTGGTTTATAAGTGCTGAAGAGCTGGATTCCTTGTCACCATACATGAGAGGAAGGCTTACACTAGAGAAGGTCAATGCAGCTATTAGTGACATGGCAACATATGCTGAAGCAAATTATCAGCCCGTAGCTGCTCCACAGAAGAATGTTTGTGCTCTGCCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

240

Amino Acids

26.24

Weight (kDa)

5.4

Isoelectric Point (pI)

41.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 9 - 233 6.1e-35 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 563
AccI GTMKAC 1 cut(s) 180
AccII CGCG 1 cut(s) 237
AciI CCGC 2 cut(s) 186, 452
AclWI GGATC 4 cut(s) 68, 323, 425, 438
AcoI YGGCCR 2 cut(s) 174, 513
AcsI RAATTY 1 cut(s) 352
AcuI CTGAAG 4 cut(s) 191, 308, 591, 681
AcyI GRCGYC 1 cut(s) 183
AfaI GTAC 1 cut(s) 462
AflIII ACRYGT 1 cut(s) 312
AgsI TTSAA 6 cut(s) 52, 131, 136, 307, 352, 418
AjiI CACGTC 1 cut(s) 546
AjnI CCWGG 2 cut(s) 213, 228
AleI CACNNNNGTG 1 cut(s) 553
AluBI AGCT 7 cut(s) 70, 205, 387, 421, 577, 635, 683
AluI AGCT 7 cut(s) 70, 205, 387, 421, 577, 635, 683
Alw21I GWGCWC 2 cut(s) 72, 709
Alw26I GTCTC 1 cut(s) 148
AlwI GGATC 4 cut(s) 68, 323, 425, 438
AoxI GGCC 4 cut(s) 174, 195, 216, 513
ApeKI GCWGC 3 cut(s) 292, 632, 683
ApoI RAATTY 1 cut(s) 352
AspS9I GGNCC 3 cut(s) 211, 226, 411
AsuHPI GGTGA 3 cut(s) 154, 540, 582
AvaII GGWCC 3 cut(s) 211, 226, 411
AxyI CCTNAGG 1 cut(s) 456
BalI TGGCCA 1 cut(s) 515
BamHI GGATCC 1 cut(s) 430
BanII GRGCYC 1 cut(s) 72
Bbv12I GWGCWC 2 cut(s) 72, 709
BbvI GCAGC 3 cut(s) 304, 644, 670
BccI CCATC 2 cut(s) 227, 260
BceAI ACGGC 3 cut(s) 161, 173, 189
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BciT130I CCWGG 2 cut(s) 215, 230
BciVI GTATCC 1 cut(s) 352
BclI TGATCA 1 cut(s) 39
BcoDI GTCTC 1 cut(s) 148
BfaI CTAG 1 cut(s) 617
BfuI GTATCC 1 cut(s) 352
BglI GCCNNNNNGGC 1 cut(s) 194
BisI GCNGC 4 cut(s) 186, 293, 633, 684
BlsI GCNGC 4 cut(s) 187, 294, 634, 685
Bme1390I CCNGG 2 cut(s) 215, 230
Bme18I GGWCC 3 cut(s) 211, 226, 411
BmgBI CACGTC 1 cut(s) 546
BmgT120I GGNCC 3 cut(s) 211, 226, 411
BmiI GGNNCC 2 cut(s) 412, 432
BmrFI CCNGG 2 cut(s) 215, 230
BmrI ACTGGG 1 cut(s) 54
BmsI GCATC 2 cut(s) 268, 290
BmuI ACTGGG 1 cut(s) 54
BoxI GACNNNNGTC 1 cut(s) 176
Bsa29I ATCGAT 1 cut(s) 276
BsaBI GATNNNNATC 1 cut(s) 429
BsaHI GRCGYC 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 198
Bse1I ACTGG 1 cut(s) 49
Bse21I CCTNAGG 1 cut(s) 456
Bse8I GATNNNNATC 1 cut(s) 429
BseBI CCWGG 2 cut(s) 215, 230
BseCI ATCGAT 1 cut(s) 276
BseDI CCNNGG 1 cut(s) 198
BseGI GGATG 2 cut(s) 252, 345
BseJI GATNNNNATC 1 cut(s) 429
BseMII CTCAG 1 cut(s) 404
BseNI ACTGG 1 cut(s) 49
BseX3I CGGCCG 1 cut(s) 174
BseXI GCAGC 3 cut(s) 304, 644, 670
BseYI CCCAGC 1 cut(s) 118
BsgI GTGCAG 1 cut(s) 420
Bsh1236I CGCG 1 cut(s) 237
Bsh1285I CGRYCG 1 cut(s) 177
BshFI GGCC 4 cut(s) 176, 197, 218, 515
BshVI ATCGAT 1 cut(s) 276
BsiEI CGRYCG 1 cut(s) 177
BsiHKAI GWGCWC 2 cut(s) 72, 709
BslFI GGGAC 1 cut(s) 424
BsmAI GTCTC 1 cut(s) 148
BsmBI CGTCTC 1 cut(s) 148
BsmFI GGGAC 1 cut(s) 424
BsnI GGCC 4 cut(s) 176, 197, 218, 515
Bsp1286I GDGCHC 2 cut(s) 72, 709
Bsp143I GATC 5 cut(s) 39, 60, 273, 328, 430
Bsp19I CCATGG 1 cut(s) 198
Bsp68I TCGCGA 1 cut(s) 237
BspACI CCGC 2 cut(s) 186, 452
BspANI GGCC 4 cut(s) 176, 197, 218, 515
BspCNI CTCAG 1 cut(s) 403
BspDI ATCGAT 1 cut(s) 276
BspFNI CGCG 1 cut(s) 237
BspHI TCATGA 1 cut(s) 325
BspLI GGNNCC 2 cut(s) 412, 432
BspPI GGATC 4 cut(s) 68, 323, 425, 438
BspQI GCTCTTC 1 cut(s) 567
BsrI ACTGG 1 cut(s) 49
BssECI CCNNGG 1 cut(s) 198
BssMI GATC 5 cut(s) 39, 60, 273, 328, 430
BssNI GRCGYC 1 cut(s) 183
BssT1I CCWWGG 1 cut(s) 198
Bst2UI CCWGG 2 cut(s) 215, 230
Bst4CI ACNGT 3 cut(s) 56, 98, 160
Bst6I CTCTTC 1 cut(s) 567
BstACI GRCGYC 1 cut(s) 183
BstAPI GCANNNNNTGC 1 cut(s) 656
BstDEI CTNAG 3 cut(s) 9, 390, 456
BstDSI CCRYGG 1 cut(s) 198
BstF5I GGATG 2 cut(s) 252, 345
BstFNI CGCG 1 cut(s) 237
BstKTI GATC 5 cut(s) 42, 63, 276, 331, 433
BstMAI GTCTC 1 cut(s) 148
BstMBI GATC 5 cut(s) 39, 60, 273, 328, 430
BstMCI CGRYCG 1 cut(s) 177
BstMWI GCNNNNNNNGC 5 cut(s) 14, 182, 194, 292, 656
BstNI CCWGG 2 cut(s) 215, 230
BstNSI RCATGY 1 cut(s) 316
BstPAI GACNNNNGTC 1 cut(s) 176
BstSCI CCNGG 2 cut(s) 213, 228
BstUI CGCG 1 cut(s) 237
BstV1I GCAGC 3 cut(s) 304, 644, 670
BstX2I RGATCY 2 cut(s) 60, 430
BstYI RGATCY 2 cut(s) 60, 430
BstZI CGGCCG 1 cut(s) 174
Bsu15I ATCGAT 1 cut(s) 276
Bsu36I CCTNAGG 1 cut(s) 456
BsuI GTATCC 1 cut(s) 352
BsuRI GGCC 4 cut(s) 176, 197, 218, 515
BsuTUI ATCGAT 1 cut(s) 276
BtgI CCRYGG 1 cut(s) 198
BtgZI GCGATG 1 cut(s) 267
BtrI CACGTC 1 cut(s) 546
BtsCI GGATG 2 cut(s) 252, 345
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 2 cut(s) 42, 399
BtuMI TCGCGA 1 cut(s) 237
CciI TCATGA 1 cut(s) 325
Cfr13I GGNCC 3 cut(s) 211, 226, 411
ClaI ATCGAT 1 cut(s) 276
CseI GACGC 2 cut(s) 12, 191
CsiI ACCWGGT 1 cut(s) 228
Csp6I GTAC 1 cut(s) 461
CviAII CATG 5 cut(s) 199, 313, 326, 598, 646
CviQI GTAC 1 cut(s) 461
DdeI CTNAG 3 cut(s) 9, 390, 456
DpnI GATC 5 cut(s) 41, 62, 275, 330, 432
DpnII GATC 5 cut(s) 39, 60, 273, 328, 430
EaeI YGGCCR 2 cut(s) 174, 513
EagI CGGCCG 1 cut(s) 174
Eam1104I CTCTTC 1 cut(s) 567
EarI CTCTTC 1 cut(s) 567
Ecl136II GAGCTC 1 cut(s) 70
EclXI CGGCCG 1 cut(s) 174
Eco130I CCWWGG 1 cut(s) 198
Eco24I GRGCYC 1 cut(s) 72
Eco47I GGWCC 3 cut(s) 211, 226, 411
Eco52I CGGCCG 1 cut(s) 174
Eco53kI GAGCTC 1 cut(s) 70
Eco57I CTGAAG 4 cut(s) 191, 308, 591, 681
Eco81I CCTNAGG 1 cut(s) 456
EcoICRI GAGCTC 1 cut(s) 70
EcoO109I RGGNCCY 2 cut(s) 226, 411
EcoRII CCWGG 2 cut(s) 213, 228
EcoT14I CCWWGG 1 cut(s) 198
EcoT22I ATGCAT 1 cut(s) 283
EcoT38I GRGCYC 1 cut(s) 72
ErhI CCWWGG 1 cut(s) 198
Esp3I CGTCTC 1 cut(s) 148
FaeI CATG 5 cut(s) 202, 316, 329, 601, 649
FaiI YATR 9 cut(s) 200, 314, 327, 563, 595, 599, 647, 655, 657
FaqI GGGAC 1 cut(s) 424
FatI CATG 5 cut(s) 198, 312, 325, 597, 645
FauNDI CATATG 1 cut(s) 655
FbaI TGATCA 1 cut(s) 39
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 4 cut(s) 186, 293, 633, 684
FokI GGATG 2 cut(s) 239, 352
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 4 cut(s) 186, 293, 633, 684
FspBI CTAG 1 cut(s) 617
GluI GCNGC 4 cut(s) 186, 293, 633, 684
GsaI CCCAGC 1 cut(s) 122
HaeIII GGCC 4 cut(s) 176, 197, 218, 515
HgaI GACGC 2 cut(s) 12, 191
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 5 cut(s) 202, 316, 329, 601, 649
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HindIII AAGCTT 1 cut(s) 419
HinfI GANTC 2 cut(s) 32, 581
HphI GGTGA 3 cut(s) 154, 540, 582
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 4 cut(s) 60, 67, 102, 719
Hpy188III TCNNGA 4 cut(s) 236, 326, 332, 380
Hpy8I GTNNAC 1 cut(s) 181
Hpy99I CGWCG 4 cut(s) 28, 182, 185, 242
HpyAV CCTTC 5 cut(s) 187, 301, 424, 600, 616
HpyCH4III ACNGT 3 cut(s) 56, 98, 160
HpyCH4IV ACGT 1 cut(s) 545
HpyCH4V TGCA 3 cut(s) 281, 437, 632
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 182, 194, 292, 656
HpyF3I CTNAG 3 cut(s) 9, 390, 456
HpySE526I ACGT 1 cut(s) 545
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 5 cut(s) 202, 316, 329, 601, 649
Ksp22I TGATCA 1 cut(s) 39
Kzo9I GATC 5 cut(s) 39, 60, 273, 328, 430
LguI GCTCTTC 1 cut(s) 567
LmnI GCTCC 5 cut(s) 20, 67, 75, 202, 691
Lsp1109I GCAGC 3 cut(s) 304, 644, 670
LweI GCATC 2 cut(s) 268, 290
MabI ACCWGGT 1 cut(s) 228
MaeI CTAG 1 cut(s) 617
MaeII ACGT 1 cut(s) 545
MaeIII GTNAC 4 cut(s) 160, 546, 588, 641
MalI GATC 5 cut(s) 41, 62, 275, 330, 432
MboI GATC 5 cut(s) 39, 60, 273, 328, 430
MboII GAAGA 2 cut(s) 584, 706
MflI RGATCY 2 cut(s) 60, 430
MhlI GDGCHC 2 cut(s) 72, 709
MlsI TGGCCA 1 cut(s) 515
MluCI AATT 6 cut(s) 131, 136, 302, 352, 538, 667
MluNI TGGCCA 1 cut(s) 515
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 2 cut(s) 9, 38
MnlI CCTC 3 cut(s) 257, 421, 596
Mox20I TGGCCA 1 cut(s) 515
Mph1103I ATGCAT 1 cut(s) 283
MscI TGGCCA 1 cut(s) 515
MseI TTAA 1 cut(s) 537
MslI CAYNNNNRTG 1 cut(s) 553
Msp20I TGGCCA 1 cut(s) 515
MspR9I CCNGG 2 cut(s) 215, 230
MvaI CCWGG 2 cut(s) 215, 230
MvnI CGCG 1 cut(s) 237
MwoI GCNNNNNNNGC 5 cut(s) 14, 182, 194, 292, 656
NcoI CCATGG 1 cut(s) 198
NdeI CATATG 1 cut(s) 655
NdeII GATC 5 cut(s) 39, 60, 273, 328, 430
NlaIII CATG 5 cut(s) 202, 316, 329, 601, 649
NlaIV GGNNCC 2 cut(s) 412, 432
NmuCI GTSAC 4 cut(s) 160, 546, 588, 641
NruI TCGCGA 1 cut(s) 237
NsiI ATGCAT 1 cut(s) 283
NspI RCATGY 1 cut(s) 316
OliI CACNNNNGTG 1 cut(s) 553
PagI TCATGA 1 cut(s) 325
PciI ACATGT 1 cut(s) 312
PciSI GCTCTTC 1 cut(s) 567
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PfeI GAWTC 1 cut(s) 581
PkrI GCNGC 4 cut(s) 187, 294, 634, 685
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PpuMI RGGWCCY 2 cut(s) 226, 411
PscI ACATGT 1 cut(s) 312
PshAI GACNNNNGTC 1 cut(s) 176
PsiI TTATAA 1 cut(s) 563
Psp124BI GAGCTC 1 cut(s) 72
Psp5II RGGWCCY 2 cut(s) 226, 411
Psp6I CCWGG 2 cut(s) 213, 228
PspFI CCCAGC 1 cut(s) 118
PspGI CCWGG 2 cut(s) 213, 228
PspN4I GGNNCC 2 cut(s) 412, 432
PspPI GGNCC 3 cut(s) 211, 226, 411
PspPPI RGGWCCY 2 cut(s) 226, 411
PsuI RGATCY 2 cut(s) 60, 430
RruI TCGCGA 1 cut(s) 237
RsaI GTAC 1 cut(s) 462
RsaNI GTAC 1 cut(s) 461
RseI CAYNNNNRTG 1 cut(s) 553
SacI GAGCTC 1 cut(s) 72
SalI GTCGAC 1 cut(s) 179
SapI GCTCTTC 1 cut(s) 567
SaqAI TTAA 1 cut(s) 537
SatI GCNGC 4 cut(s) 186, 293, 633, 684
Sau3AI GATC 5 cut(s) 39, 60, 273, 328, 430
Sau96I GGNCC 3 cut(s) 211, 226, 411
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 2 cut(s) 215, 230
SduI GDGCHC 2 cut(s) 72, 709
SexAI ACCWGGT 1 cut(s) 228
SfaNI GCATC 2 cut(s) 268, 290
SgrDI CGTCGACG 1 cut(s) 179
SinI GGWCC 3 cut(s) 211, 226, 411
SmiMI CAYNNNNRTG 1 cut(s) 553
Sse9I AATT 6 cut(s) 131, 136, 302, 352, 538, 667
SsiI CCGC 2 cut(s) 186, 452
SspMI CTAG 1 cut(s) 617
SstI GAGCTC 1 cut(s) 72
StyD4I CCNGG 2 cut(s) 213, 228
StyI CCWWGG 1 cut(s) 198
TaaI ACNGT 3 cut(s) 56, 98, 160
TaiI ACGT 1 cut(s) 548
TaqI TCGA 3 cut(s) 91, 180, 276
TasI AATT 6 cut(s) 131, 136, 302, 352, 538, 667
TauI GCSGC 1 cut(s) 188
TfiI GAWTC 1 cut(s) 581
Tru1I TTAA 1 cut(s) 537
Tru9I TTAA 1 cut(s) 537
TscAI CASTG 2 cut(s) 49, 406
TseFI GTSAC 4 cut(s) 160, 546, 588, 641
TseI GCWGC 3 cut(s) 292, 632, 683
Tsp45I GTSAC 4 cut(s) 160, 546, 588, 641
TspRI CASTG 2 cut(s) 49, 406
VpaK11BI GGWCC 3 cut(s) 211, 226, 411
XapI RAATTY 1 cut(s) 352
XceI RCATGY 1 cut(s) 316
XcmI CCANNNNNNNNNTGG 1 cut(s) 226
XmiI GTMKAC 1 cut(s) 180
XspI CTAG 1 cut(s) 617
Zsp2I ATGCAT 1 cut(s) 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.