Rroxscaffold_4G00305770

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001324
Physical Location & Seq
Reverse (-)
26414050 .. 26418107
4058 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00305770.1

Sequence Viewer

Length: 819 bp
ATGGAGGCGGAGAAAGCAGGGGCGTCGTTGGACTCGCTGATCGCCGGGTTCAACAGTCGGATCTCGGAGCTCCAGGAGCTCGTTATTGCCCGAAACATGTACCCGGCCAGCAGCATCACCGATTTGACGGCCGTCGACGCCGCAGTGAAGGCCATGGAGCTTCAGGTCCAGACCATCAAGGACCGGGTTCGCGACGAAACCCTAGCCATCCCCAAAGCAAAAAAACTGATCGATGCATCCCTGAAGCAGCAAAGGAAATTGAAGGATATGTCAATTTATGCCCCCACTCATGTTCCTGAAAGAATGTCGATGTTGAATTTGGATACCAATAGATGTTTATTTCCAGAAAGCTCTCAGCAAAACACTGGTTCTGGGACCTTCAAGCTTGAGGAGGATCCTGCACCACCACCACCACCACCAAAGGAGAAAAAGGGTCGTTCCACGCCACCATTGTGGTTTGTAAGTGCTGAAGAGCTGGATTCCTTGTCATCGTACATGAGAGGAAGGCTTACACTAGAGAAGGTGAATGCAGCTATTAATGACATGGCAACATATGCTGAAGCAAATTATCAGCTTATAGCTGCTCCCAAGAAGAATGTGGCAGGAGACCGTTGGGAAAAGGCTCTGGAAATAAGAGATATAGCAATGCATGAAGCAGTGAAGGGAAAACACTTCTTTCTTGAAACTGACATGAAAGGACCATCCTTGAAGCTTGACAACTCTGGAAAAGCAATACTAACTGTCCTTCGTCACCTTGGTCGTGTAAGTGAGACACGGATTGGTCATCATCGGGTGATCATTCTTTTGAAGCCTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

272

Amino Acids

30.25

Weight (kDa)

8.99

Isoelectric Point (pI)

46.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 9 - 262 7.6e-85 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 135
AccII CGCG 1 cut(s) 192
AciI CCGC 2 cut(s) 8, 141
AclWI GGATC 3 cut(s) 68, 389, 402
AcoI YGGCCR 2 cut(s) 105, 129
AcsI RAATTY 1 cut(s) 316
AcuI CTGAAG 4 cut(s) 146, 263, 489, 579
AcyI GRCGYC 2 cut(s) 23, 138
AfaI GTAC 2 cut(s) 101, 494
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 7 cut(s) 52, 262, 316, 382, 683, 709, 808
AjnI CCWGG 1 cut(s) 72
AleI CACNNNNGTG 1 cut(s) 451
Alw21I GWGCWC 2 cut(s) 72, 81
Alw26I GTCTC 2 cut(s) 600, 764
AlwI GGATC 3 cut(s) 68, 389, 402
AoxI GGCC 3 cut(s) 105, 129, 150
ApeKI GCWGC 4 cut(s) 111, 247, 530, 581
ApoI RAATTY 1 cut(s) 316
AseI ATTAAT 1 cut(s) 537
AspS9I GGNCC 4 cut(s) 166, 181, 375, 698
AsuC2I CCSGG 3 cut(s) 46, 104, 185
AsuHPI GGTGA 4 cut(s) 109, 535, 743, 805
AvaII GGWCC 4 cut(s) 166, 181, 375, 698
BamHI GGATCC 1 cut(s) 394
BanII GRGCYC 2 cut(s) 72, 81
Bbv12I GWGCWC 2 cut(s) 72, 81
BbvI GCAGC 4 cut(s) 123, 259, 542, 568
BccI CCATC 3 cut(s) 182, 215, 709
BceAI ACGGC 2 cut(s) 116, 144
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BciT130I CCWGG 1 cut(s) 74
BciVI GTATCC 1 cut(s) 316
BclI TGATCA 1 cut(s) 795
BcnI CCSGG 3 cut(s) 46, 104, 185
BcoDI GTCTC 2 cut(s) 600, 764
BfaI CTAG 2 cut(s) 203, 515
BfuI GTATCC 1 cut(s) 316
BisI GCNGC 5 cut(s) 112, 141, 248, 531, 582
BlsI GCNGC 5 cut(s) 113, 142, 249, 532, 583
Bme1390I CCNGG 4 cut(s) 46, 74, 104, 185
Bme18I GGWCC 4 cut(s) 166, 181, 375, 698
BmgT120I GGNCC 4 cut(s) 166, 181, 375, 698
BmiI GGNNCC 2 cut(s) 376, 396
BmrFI CCNGG 4 cut(s) 46, 74, 104, 185
BmsI GCATC 3 cut(s) 123, 223, 245
BoxI GACNNNNGTC 1 cut(s) 131
BpmI CTGGAG 1 cut(s) 56
BpuEI CTTGAG 1 cut(s) 407
BpuMI CCSGG 3 cut(s) 46, 104, 185
Bsa29I ATCGAT 1 cut(s) 231
BsaHI GRCGYC 2 cut(s) 23, 138
BsaI GGTCTC 1 cut(s) 600
BsaJI CCNNGG 2 cut(s) 153, 754
Bse1I ACTGG 1 cut(s) 370
Bse3DI GCAATG 1 cut(s) 651
BseBI CCWGG 1 cut(s) 74
BseCI ATCGAT 1 cut(s) 231
BseDI CCNNGG 2 cut(s) 153, 754
BseGI GGATG 3 cut(s) 207, 236, 701
BseMI GCAATG 1 cut(s) 651
BseMII CTCAG 1 cut(s) 368
BseNI ACTGG 1 cut(s) 370
BseRI GAGGAG 1 cut(s) 404
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 4 cut(s) 123, 259, 542, 568
BsgI GTGCAG 1 cut(s) 384
Bsh1236I CGCG 1 cut(s) 192
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 3 cut(s) 107, 131, 152
BshVI ATCGAT 1 cut(s) 231
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 2 cut(s) 72, 81
BsiSI CCGG 3 cut(s) 45, 104, 184
BslFI GGGAC 1 cut(s) 388
BsmAI GTCTC 2 cut(s) 600, 764
BsmFI GGGAC 1 cut(s) 388
BsmI GAATGC 1 cut(s) 532
BsnI GGCC 3 cut(s) 107, 131, 152
Bso31I GGTCTC 1 cut(s) 600
Bsp1286I GDGCHC 2 cut(s) 72, 81
Bsp143I GATC 5 cut(s) 39, 60, 228, 394, 795
Bsp19I CCATGG 1 cut(s) 153
Bsp68I TCGCGA 1 cut(s) 192
BspACI CCGC 2 cut(s) 8, 141
BspANI GGCC 3 cut(s) 107, 131, 152
BspCNI CTCAG 1 cut(s) 367
BspDI ATCGAT 1 cut(s) 231
BspFNI CGCG 1 cut(s) 192
BspLI GGNNCC 2 cut(s) 376, 396
BspPI GGATC 3 cut(s) 68, 389, 402
BspQI GCTCTTC 1 cut(s) 465
BspTNI GGTCTC 1 cut(s) 600
BsrDI GCAATG 1 cut(s) 651
BsrI ACTGG 1 cut(s) 370
BssECI CCNNGG 2 cut(s) 153, 754
BssMI GATC 5 cut(s) 39, 60, 228, 394, 795
BssNI GRCGYC 2 cut(s) 23, 138
BssT1I CCWWGG 2 cut(s) 153, 754
Bst2UI CCWGG 1 cut(s) 74
Bst4CI ACNGT 3 cut(s) 56, 611, 742
Bst6I CTCTTC 1 cut(s) 465
BstACI GRCGYC 2 cut(s) 23, 138
BstAPI GCANNNNNTGC 1 cut(s) 554
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 1 cut(s) 354
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 3 cut(s) 207, 236, 701
BstFNI CGCG 1 cut(s) 192
BstKTI GATC 5 cut(s) 42, 63, 231, 397, 798
BstMAI GTCTC 2 cut(s) 600, 764
BstMBI GATC 5 cut(s) 39, 60, 228, 394, 795
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 5 cut(s) 14, 76, 137, 149, 554
BstNI CCWGG 1 cut(s) 74
BstNSI RCATGY 1 cut(s) 100
BstPAI GACNNNNGTC 1 cut(s) 131
BstSCI CCNGG 4 cut(s) 44, 72, 102, 183
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 4 cut(s) 123, 259, 542, 568
BstX2I RGATCY 2 cut(s) 60, 394
BstXI CCANNNNNNTGG 1 cut(s) 453
BstYI RGATCY 2 cut(s) 60, 394
BstZI CGGCCG 1 cut(s) 129
Bsu15I ATCGAT 1 cut(s) 231
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 3 cut(s) 107, 131, 152
BsuTUI ATCGAT 1 cut(s) 231
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 3 cut(s) 207, 236, 701
BtsI GCAGTG 2 cut(s) 150, 663
BtsIMutI CAGTG 3 cut(s) 150, 363, 663
BtuMI TCGCGA 1 cut(s) 192
Cac8I GCNNGC 1 cut(s) 109
Cfr13I GGNCC 4 cut(s) 166, 181, 375, 698
ClaI ATCGAT 1 cut(s) 231
CseI GACGC 2 cut(s) 12, 146
Csp6I GTAC 2 cut(s) 100, 493
CviAII CATG 7 cut(s) 97, 154, 290, 496, 544, 650, 691
CviQI GTAC 2 cut(s) 100, 493
DdeI CTNAG 1 cut(s) 354
DpnI GATC 5 cut(s) 41, 62, 230, 396, 797
DpnII GATC 5 cut(s) 39, 60, 228, 394, 795
EaeI YGGCCR 2 cut(s) 105, 129
EagI CGGCCG 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 465
EarI CTCTTC 1 cut(s) 465
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 2 cut(s) 70, 79
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 2 cut(s) 153, 754
Eco24I GRGCYC 2 cut(s) 72, 81
Eco31I GGTCTC 1 cut(s) 600
Eco47I GGWCC 4 cut(s) 166, 181, 375, 698
Eco52I CGGCCG 1 cut(s) 129
Eco53kI GAGCTC 2 cut(s) 70, 79
Eco57I CTGAAG 4 cut(s) 146, 263, 489, 579
EcoICRI GAGCTC 2 cut(s) 70, 79
EcoO109I RGGNCCY 1 cut(s) 375
EcoRII CCWGG 1 cut(s) 72
EcoT14I CCWWGG 2 cut(s) 153, 754
EcoT22I ATGCAT 2 cut(s) 238, 651
EcoT38I GRGCYC 2 cut(s) 72, 81
ErhI CCWWGG 2 cut(s) 153, 754
FaeI CATG 7 cut(s) 100, 157, 293, 499, 547, 653, 694
FaqI GGGAC 1 cut(s) 388
FatI CATG 7 cut(s) 96, 153, 289, 495, 543, 649, 690
FauNDI CATATG 1 cut(s) 553
FbaI TGATCA 1 cut(s) 795
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 5 cut(s) 112, 141, 248, 531, 582
FokI GGATG 3 cut(s) 194, 223, 688
FriOI GRGCYC 2 cut(s) 72, 81
Fsp4HI GCNGC 5 cut(s) 112, 141, 248, 531, 582
FspBI CTAG 2 cut(s) 203, 515
GluI GCNGC 5 cut(s) 112, 141, 248, 531, 582
GsuI CTGGAG 1 cut(s) 56
HaeIII GGCC 3 cut(s) 107, 131, 152
HapII CCGG 3 cut(s) 45, 104, 184
HgaI GACGC 2 cut(s) 12, 146
Hin1I GRCGYC 2 cut(s) 23, 138
Hin1II CATG 7 cut(s) 100, 157, 293, 499, 547, 653, 694
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HindIII AAGCTT 2 cut(s) 383, 710
HinfI GANTC 2 cut(s) 32, 479
HpaII CCGG 3 cut(s) 45, 104, 184
HphI GGTGA 4 cut(s) 109, 535, 743, 805
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 2 cut(s) 60, 67
Hpy188III TCNNGA 7 cut(s) 169, 191, 296, 344, 626, 680, 723
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 4 cut(s) 28, 137, 140, 197
HpyAV CCTTC 7 cut(s) 142, 256, 388, 498, 514, 655, 755
HpyCH4III ACNGT 3 cut(s) 56, 611, 742
HpyCH4V TGCA 4 cut(s) 236, 401, 530, 649
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 76, 137, 149, 554
HpyF3I CTNAG 1 cut(s) 354
Hsp92I GRCGYC 2 cut(s) 23, 138
Hsp92II CATG 7 cut(s) 100, 157, 293, 499, 547, 653, 694
Ksp22I TGATCA 1 cut(s) 795
Kzo9I GATC 5 cut(s) 39, 60, 228, 394, 795
LguI GCTCTTC 1 cut(s) 465
LmnI GCTCC 5 cut(s) 67, 75, 76, 157, 589
Lsp1109I GCAGC 4 cut(s) 123, 259, 542, 568
LweI GCATC 3 cut(s) 123, 223, 245
MaeI CTAG 2 cut(s) 203, 515
MaeIII GTNAC 1 cut(s) 749
MalI GATC 5 cut(s) 41, 62, 230, 396, 797
MboI GATC 5 cut(s) 39, 60, 228, 394, 795
MboII GAAGA 2 cut(s) 482, 604
MflI RGATCY 2 cut(s) 60, 394
MhlI GDGCHC 2 cut(s) 72, 81
MluCI AATT 4 cut(s) 257, 273, 316, 565
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 2 cut(s) 9, 38
MnlI CCTC 3 cut(s) 382, 385, 494
Mph1103I ATGCAT 2 cut(s) 238, 651
MseI TTAA 1 cut(s) 537
MslI CAYNNNNRTG 1 cut(s) 451
MspI CCGG 3 cut(s) 45, 104, 184
MspR9I CCNGG 4 cut(s) 46, 74, 104, 185
Mva1269I GAATGC 1 cut(s) 532
MvaI CCWGG 1 cut(s) 74
MvnI CGCG 1 cut(s) 192
MwoI GCNNNNNNNGC 5 cut(s) 14, 76, 137, 149, 554
NciI CCSGG 3 cut(s) 46, 104, 185
NcoI CCATGG 1 cut(s) 153
NdeI CATATG 1 cut(s) 553
NdeII GATC 5 cut(s) 39, 60, 228, 394, 795
NlaIII CATG 7 cut(s) 100, 157, 293, 499, 547, 653, 694
NlaIV GGNNCC 2 cut(s) 376, 396
NmuCI GTSAC 1 cut(s) 749
NruI TCGCGA 1 cut(s) 192
NsiI ATGCAT 2 cut(s) 238, 651
NspI RCATGY 1 cut(s) 100
OliI CACNNNNGTG 1 cut(s) 451
PciI ACATGT 1 cut(s) 96
PciSI GCTCTTC 1 cut(s) 465
PctI GAATGC 1 cut(s) 532
PfeI GAWTC 1 cut(s) 479
PfoI TCCNGGA 1 cut(s) 72
PkrI GCNGC 5 cut(s) 113, 142, 249, 532, 583
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PpuMI RGGWCCY 1 cut(s) 375
PscI ACATGT 1 cut(s) 96
PshAI GACNNNNGTC 1 cut(s) 131
PshBI ATTAAT 1 cut(s) 537
Psp124BI GAGCTC 2 cut(s) 72, 81
Psp5II RGGWCCY 1 cut(s) 375
Psp6I CCWGG 1 cut(s) 72
PspGI CCWGG 1 cut(s) 72
PspN4I GGNNCC 2 cut(s) 376, 396
PspPI GGNCC 4 cut(s) 166, 181, 375, 698
PspPPI RGGWCCY 1 cut(s) 375
PsuI RGATCY 2 cut(s) 60, 394
RruI TCGCGA 1 cut(s) 192
RsaI GTAC 2 cut(s) 101, 494
RsaNI GTAC 2 cut(s) 100, 493
RseI CAYNNNNRTG 1 cut(s) 451
SacI GAGCTC 2 cut(s) 72, 81
SalI GTCGAC 1 cut(s) 134
SapI GCTCTTC 1 cut(s) 465
SaqAI TTAA 1 cut(s) 537
SatI GCNGC 5 cut(s) 112, 141, 248, 531, 582
Sau3AI GATC 5 cut(s) 39, 60, 228, 394, 795
Sau96I GGNCC 4 cut(s) 166, 181, 375, 698
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 4 cut(s) 46, 74, 104, 185
SduI GDGCHC 2 cut(s) 72, 81
SfaNI GCATC 3 cut(s) 123, 223, 245
SgrDI CGTCGACG 1 cut(s) 134
SinI GGWCC 4 cut(s) 166, 181, 375, 698
SmiMI CAYNNNNRTG 1 cut(s) 451
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
Sse9I AATT 4 cut(s) 257, 273, 316, 565
SsiI CCGC 2 cut(s) 8, 141
SspMI CTAG 2 cut(s) 203, 515
SstI GAGCTC 2 cut(s) 72, 81
StyD4I CCNGG 4 cut(s) 44, 72, 102, 183
StyI CCWWGG 2 cut(s) 153, 754
TaaI ACNGT 3 cut(s) 56, 611, 742
TaqI TCGA 3 cut(s) 135, 231, 308
TasI AATT 4 cut(s) 257, 273, 316, 565
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 1 cut(s) 479
Tru1I TTAA 1 cut(s) 537
Tru9I TTAA 1 cut(s) 537
TscAI CASTG 3 cut(s) 150, 370, 663
TseFI GTSAC 1 cut(s) 749
TseI GCWGC 4 cut(s) 111, 247, 530, 581
Tsp45I GTSAC 1 cut(s) 749
TspDTI ATGAA 2 cut(s) 666, 707
TspGWI ACGGA 1 cut(s) 790
TspRI CASTG 3 cut(s) 150, 370, 663
VpaK11BI GGWCC 4 cut(s) 166, 181, 375, 698
VspI ATTAAT 1 cut(s) 537
XapI RAATTY 1 cut(s) 316
XceI RCATGY 1 cut(s) 100
XcmI CCANNNNNNNNNTGG 1 cut(s) 595
XmiI GTMKAC 1 cut(s) 135
XspI CTAG 2 cut(s) 203, 515
Zsp2I ATGCAT 2 cut(s) 238, 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.