Rorug01G0201900

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001324
Physical Location & Seq
Forward (+)
28106389 .. 28106979
591 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0201900.1

Sequence Viewer

Length: 591 bp
ATGACCATATTAGTTCTTGCTCTGGCCACCTCCAAAAGGGTCTTGGCAAGTGATCCGGATATCCTTTCAGACTTTTTACTCCCACAAAACAGCTCAACAGTTGATGCTAGTTTTTTTACATTCACTGGATTTCGCGGAATATTTGATCAAGCACCCAAAACCTTGAAGACAACAAAAGCCAGCATGGTTGAGTTTCCTGCTCTCAATGGCCAGAGTGTGTCATACGCGGTGCTTCAATTGCCTCCACAGACACTCTTCCCACCTAATACTCGCCCTGATGCAACTGGACTTCTGTTCCTTTTGGATGGTTCCTTGGAAGTAGGCCTAATAGACACAACAATGAATCTGTATACTCAAAAGCTTCAGGCAGGAGATATGTTTGTATTTCCCAAGGGATTAATACATTATCAGTATAACACTGATTCTCAATTGCCAGCCACCGCCATTGCAGCATTCGGAAGTGCAAGTGCTAGAGCAGTTGCGGTGCCACCTTCTGTTTTTGCTTCAGGTATTGACGATGTCATCCTTGCCAAATCCTTCAAGACAGATGTTGATACTATTAAGAAGATTAAGGCTGGCCTCACTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

196

Amino Acids

20.94

Weight (kDa)

5.72

Isoelectric Point (pI)

31.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 55 - 187 4.9e-25 Cupin
Cupin_2 PF07883 78 - 150 1.4e-06 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 484
AccI GTMKAC 1 cut(s) 350
AccII CGCG 2 cut(s) 135, 227
AccIII TCCGGA 1 cut(s) 55
AciI CCGC 4 cut(s) 135, 227, 441, 482
AclWI GGATC 1 cut(s) 47
AcoI YGGCCR 2 cut(s) 24, 208
AcuI CTGAAG 2 cut(s) 347, 489
AfiI CCNNNNNNNGG 1 cut(s) 36
AgsI TTSAA 3 cut(s) 166, 236, 541
AluBI AGCT 3 cut(s) 93, 361, 588
AluI AGCT 3 cut(s) 93, 361, 588
AlwI GGATC 1 cut(s) 47
Aor13HI TCCGGA 1 cut(s) 55
AoxI GGCC 4 cut(s) 24, 208, 322, 577
ApeKI GCWGC 1 cut(s) 449
AseI ATTAAT 1 cut(s) 398
BalI TGGCCA 2 cut(s) 26, 210
BanI GGYRCC 1 cut(s) 484
BbsI GAAGAC 1 cut(s) 173
BbvI GCAGC 1 cut(s) 461
BccI CCATC 1 cut(s) 299
BclI TGATCA 1 cut(s) 145
BfaI CTAG 3 cut(s) 108, 471, 585
BisI GCNGC 1 cut(s) 450
BlsI GCNGC 1 cut(s) 451
BmiI GGNNCC 2 cut(s) 310, 486
BmsI GCATC 2 cut(s) 94, 268
BpiI GAAGAC 1 cut(s) 173
BsaJI CCNNGG 2 cut(s) 312, 390
BsaWI WCCGGW 1 cut(s) 55
Bsc4I CCNNNNNNNGG 1 cut(s) 36
Bse1I ACTGG 2 cut(s) 130, 289
Bse3DI GCAATG 1 cut(s) 444
BseAI TCCGGA 1 cut(s) 55
BseDI CCNNGG 2 cut(s) 312, 390
BseGI GGATG 2 cut(s) 310, 522
BseLI CCNNNNNNNGG 1 cut(s) 36
BseMI GCAATG 1 cut(s) 444
BseNI ACTGG 2 cut(s) 130, 289
BseXI GCAGC 1 cut(s) 461
Bsh1236I CGCG 2 cut(s) 135, 227
BshFI GGCC 4 cut(s) 26, 210, 324, 579
BshNI GGYRCC 1 cut(s) 484
BsiSI CCGG 1 cut(s) 56
BslI CCNNNNNNNGG 1 cut(s) 36
BsmI GAATGC 1 cut(s) 452
BsnI GGCC 4 cut(s) 26, 210, 324, 579
Bsp13I TCCGGA 1 cut(s) 55
Bsp143I GATC 2 cut(s) 52, 145
BspACI CCGC 4 cut(s) 135, 227, 441, 482
BspANI GGCC 4 cut(s) 26, 210, 324, 579
BspEI TCCGGA 1 cut(s) 55
BspFNI CGCG 2 cut(s) 135, 227
BspLI GGNNCC 2 cut(s) 310, 486
BspPI GGATC 1 cut(s) 47
BspT107I GGYRCC 1 cut(s) 484
BsrDI GCAATG 1 cut(s) 444
BsrI ACTGG 2 cut(s) 130, 289
BssECI CCNNGG 2 cut(s) 312, 390
BssMI GATC 2 cut(s) 52, 145
BssNAI GTATAC 1 cut(s) 351
BssT1I CCWWGG 2 cut(s) 312, 390
Bst1107I GTATAC 1 cut(s) 351
Bst4CI ACNGT 1 cut(s) 100
Bst6I CTCTTC 1 cut(s) 260
BstC8I GCNNGC 3 cut(s) 181, 435, 577
BstENI CCTNNNNNAGG 1 cut(s) 34
BstF5I GGATG 2 cut(s) 310, 522
BstFNI CGCG 2 cut(s) 135, 227
BstKTI GATC 2 cut(s) 55, 148
BstMBI GATC 2 cut(s) 52, 145
BstMWI GCNNNNNNNGC 3 cut(s) 238, 449, 585
BstUI CGCG 2 cut(s) 135, 227
BstV1I GCAGC 1 cut(s) 461
BstV2I GAAGAC 1 cut(s) 173
BstZ17I GTATAC 1 cut(s) 351
BsuRI GGCC 4 cut(s) 26, 210, 324, 579
BtsCI GGATG 2 cut(s) 310, 522
BtsIMutI CAGTG 2 cut(s) 123, 417
Cac8I GCNNGC 3 cut(s) 181, 435, 577
CspCI CAANNNNNGTGG 2 cut(s) 427, 462
CviAII CATG 1 cut(s) 184
DpnI GATC 2 cut(s) 54, 147
DpnII GATC 2 cut(s) 52, 145
EaeI YGGCCR 2 cut(s) 24, 208
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
Eco130I CCWWGG 2 cut(s) 312, 390
Eco147I AGGCCT 1 cut(s) 324
Eco32I GATATC 1 cut(s) 61
Eco57I CTGAAG 2 cut(s) 347, 489
EcoNI CCTNNNNNAGG 1 cut(s) 34
EcoRV GATATC 1 cut(s) 61
EcoT14I CCWWGG 2 cut(s) 312, 390
ErhI CCWWGG 2 cut(s) 312, 390
FaeI CATG 1 cut(s) 187
FaiI YATR 6 cut(s) 8, 185, 223, 351, 377, 414
FatI CATG 1 cut(s) 183
FbaI TGATCA 1 cut(s) 145
FblI GTMKAC 1 cut(s) 350
Fnu4HI GCNGC 1 cut(s) 450
FokI GGATG 2 cut(s) 317, 509
Fsp4HI GCNGC 1 cut(s) 450
FspBI CTAG 3 cut(s) 108, 471, 585
GluI GCNGC 1 cut(s) 450
HaeIII GGCC 4 cut(s) 26, 210, 324, 579
HapII CCGG 1 cut(s) 56
Hin1II CATG 1 cut(s) 187
HindIII AAGCTT 1 cut(s) 359
HinfI GANTC 2 cut(s) 343, 422
HpaII CCGG 1 cut(s) 56
Hpy166II GTNNAC 1 cut(s) 351
Hpy188I TCNGA 2 cut(s) 70, 458
Hpy188III TCNNGA 2 cut(s) 56, 541
Hpy8I GTNNAC 1 cut(s) 351
HpyAV CCTTC 2 cut(s) 501, 547
HpyCH4III ACNGT 1 cut(s) 100
HpyCH4V TGCA 3 cut(s) 281, 449, 464
HpyF10VI GCNNNNNNNGC 3 cut(s) 238, 449, 585
Hsp92II CATG 1 cut(s) 187
Kpn2I TCCGGA 1 cut(s) 55
Ksp22I TGATCA 1 cut(s) 145
Kzo9I GATC 2 cut(s) 52, 145
Lsp1109I GCAGC 1 cut(s) 461
LweI GCATC 2 cut(s) 94, 268
MaeI CTAG 3 cut(s) 108, 471, 585
MalI GATC 2 cut(s) 54, 147
MboI GATC 2 cut(s) 52, 145
MboII GAAGA 3 cut(s) 178, 247, 577
MfeI CAATTG 2 cut(s) 236, 428
MlsI TGGCCA 2 cut(s) 26, 210
MluCI AATT 2 cut(s) 236, 428
MluNI TGGCCA 2 cut(s) 26, 210
MnlI CCTC 3 cut(s) 40, 252, 590
Mox20I TGGCCA 2 cut(s) 26, 210
MroI TCCGGA 1 cut(s) 55
MscI TGGCCA 2 cut(s) 26, 210
MseI TTAA 3 cut(s) 398, 561, 570
MslI CAYNNNNRTG 1 cut(s) 338
Msp20I TGGCCA 2 cut(s) 26, 210
MspI CCGG 1 cut(s) 56
MunI CAATTG 2 cut(s) 236, 428
Mva1269I GAATGC 1 cut(s) 452
MvnI CGCG 2 cut(s) 135, 227
MwoI GCNNNNNNNGC 3 cut(s) 238, 449, 585
NdeII GATC 2 cut(s) 52, 145
NlaIII CATG 1 cut(s) 187
NlaIV GGNNCC 2 cut(s) 310, 486
PceI AGGCCT 1 cut(s) 324
PctI GAATGC 1 cut(s) 452
PfeI GAWTC 2 cut(s) 343, 422
PflFI GACNNNGTC 1 cut(s) 518
PkrI GCNGC 1 cut(s) 451
PshBI ATTAAT 1 cut(s) 398
PspN4I GGNNCC 2 cut(s) 310, 486
PsyI GACNNNGTC 1 cut(s) 518
RseI CAYNNNNRTG 1 cut(s) 338
SaqAI TTAA 3 cut(s) 398, 561, 570
SatI GCNGC 1 cut(s) 450
Sau3AI GATC 2 cut(s) 52, 145
SetI ASST 8 cut(s) 32, 95, 164, 265, 363, 493, 511, 590
SfaNI GCATC 2 cut(s) 94, 268
SmiMI CAYNNNNRTG 1 cut(s) 338
Sse9I AATT 2 cut(s) 236, 428
SseBI AGGCCT 1 cut(s) 324
SsiI CCGC 4 cut(s) 135, 227, 441, 482
SspI AATATT 1 cut(s) 141
SspMI CTAG 3 cut(s) 108, 471, 585
StuI AGGCCT 1 cut(s) 324
StyI CCWWGG 2 cut(s) 312, 390
TaaI ACNGT 1 cut(s) 100
TasI AATT 2 cut(s) 236, 428
TfiI GAWTC 2 cut(s) 343, 422
Tru1I TTAA 3 cut(s) 398, 561, 570
Tru9I TTAA 3 cut(s) 398, 561, 570
TscAI CASTG 2 cut(s) 130, 424
TseI GCWGC 1 cut(s) 449
TspDTI ATGAA 1 cut(s) 356
TspRI CASTG 2 cut(s) 130, 424
Tth111I GACNNNGTC 1 cut(s) 518
VspI ATTAAT 1 cut(s) 398
XagI CCTNNNNNAGG 1 cut(s) 34
XcmI CCANNNNNNNNNTGG 1 cut(s) 40
XmiI GTMKAC 1 cut(s) 350
XspI CTAG 3 cut(s) 108, 471, 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.