Rw0G019560

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00879
OG0001324
Physical Location & Seq
Reverse (-)
9786 .. 13565
3780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G019560.1

Sequence Viewer

Length: 816 bp
ATGGAGGCGGAGAAAGCAGGGGCGTCGTTGGACTCGCTGATCGCTGGGTTCAACAGTCGGATCTCGGAGCTCCAAGAGCTCGTTATTGCCCGAAACATGTACCCGGCCAGCAGAGTCACTGATTTGACTGCCGTCGACGCCGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGCCATCAAGGACCGGGTACGCGACGAAACCCTCGCCATCCCCAAAGAAAAAAAACTGATCGATGCATCGCTGAAGCAGCAAAGGAAATTGAAGGACATGTCAATTTATGCGCCCACTCATGTTCCTGAAAGGATGTCAATGTTGAATTTGGATACAAATAGATGTTTATTTCCAGATAGCTCTCAGCAAAACACTGGTTCTGGGACCTTCAAGCTTGACGAGGATCCTGCACCACCACCAAAAAAGGGTCGTTCCACGCCACCATTGTGGTTTATAAGTGCTGAAGAGCTGGATTCCTTGTCACTATACATGAGAGGAAGGCTTACACTAGACAAGGTCAATGCAGCTATTAATGACATGGCAACATATGCTGAAGCAAATTATCAGCTCATAGCTGCTCCAAAGAAGAATGTGGCAGGAGATCGATGGGAAAAAGCCTTGGAAATAAGAGATATAGCAATGCATGAAGCAGTAAAGGGAAAACACTTCTTTCTCGAAACTGACATGAAGGGACCATCCTTGAAGCTTGACAACTCTGGAAAAGCAATACTAACTCATCATGCTTTGTCGAATTCTTCAAATGGGCCCAAATTTGGACATGGGCTTCTTTTTGTATCCTCGCGAGTCATGTTCCTCGATCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

271

Amino Acids

30.0

Weight (kDa)

7.81

Isoelectric Point (pI)

39.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 9 - 242 7.3e-72 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 446
AccI GTMKAC 1 cut(s) 135
AccII CGCG 2 cut(s) 192, 793
AciI CCGC 2 cut(s) 8, 141
AclWI GGATC 4 cut(s) 68, 389, 402, 803
AcoI YGGCCR 1 cut(s) 105
AcsI RAATTY 3 cut(s) 316, 742, 761
AcuI CTGAAG 4 cut(s) 146, 263, 474, 564
AcyI GRCGYC 2 cut(s) 23, 138
AfaI GTAC 2 cut(s) 101, 189
AfiI CCNNNNNNNGG 2 cut(s) 416, 764
AflIII ACRYGT 2 cut(s) 96, 267
AgsI TTSAA 6 cut(s) 52, 262, 316, 382, 694, 750
AjnI CCWGG 1 cut(s) 168
AleI CACNNNNGTG 1 cut(s) 436
Alw21I GWGCWC 2 cut(s) 72, 81
AlwI GGATC 4 cut(s) 68, 389, 402, 803
AoxI GGCC 4 cut(s) 105, 150, 171, 755
ApaI GGGCCC 1 cut(s) 759
ApeKI GCWGC 3 cut(s) 247, 515, 566
ApoI RAATTY 3 cut(s) 316, 742, 761
AseI ATTAAT 1 cut(s) 522
AspLEI GCGC 1 cut(s) 283
AspS9I GGNCC 6 cut(s) 166, 181, 375, 683, 755, 756
AsuC2I CCSGG 2 cut(s) 104, 185
AvaII GGWCC 4 cut(s) 166, 181, 375, 683
BaeGI GKGCMC 1 cut(s) 759
BamHI GGATCC 1 cut(s) 394
BanII GRGCYC 3 cut(s) 72, 81, 759
Bbv12I GWGCWC 2 cut(s) 72, 81
BbvI GCAGC 3 cut(s) 259, 527, 553
BccI CCATC 4 cut(s) 182, 215, 591, 694
BceAI ACGGC 2 cut(s) 116, 128
BcgI CGANNNNNNTGC 4 cut(s) 6, 40, 380, 414
BciT130I CCWGG 1 cut(s) 170
BciVI GTATCC 2 cut(s) 316, 796
BcnI CCSGG 2 cut(s) 104, 185
BfaI CTAG 1 cut(s) 500
BfuI GTATCC 2 cut(s) 316, 796
BglI GCCNNNNNGGC 1 cut(s) 149
BisI GCNGC 4 cut(s) 141, 248, 516, 567
BlsI GCNGC 4 cut(s) 142, 249, 517, 568
Bme1390I CCNGG 3 cut(s) 104, 170, 185
Bme18I GGWCC 4 cut(s) 166, 181, 375, 683
BmgT120I GGNCC 6 cut(s) 166, 181, 375, 683, 755, 756
BmiI GGNNCC 4 cut(s) 376, 396, 684, 757
BmrFI CCNGG 3 cut(s) 104, 170, 185
BmsI GCATC 2 cut(s) 223, 245
BoxI GACNNNNGTC 1 cut(s) 131
BpuMI CCSGG 2 cut(s) 104, 185
Bsa29I ATCGAT 2 cut(s) 231, 595
BsaHI GRCGYC 2 cut(s) 23, 138
BsaJI CCNNGG 2 cut(s) 153, 609
Bsc4I CCNNNNNNNGG 2 cut(s) 416, 764
Bse1I ACTGG 1 cut(s) 370
Bse3DI GCAATG 1 cut(s) 636
BseBI CCWGG 1 cut(s) 170
BseCI ATCGAT 2 cut(s) 231, 595
BseDI CCNNGG 2 cut(s) 153, 609
BseGI GGATG 3 cut(s) 207, 309, 686
BseLI CCNNNNNNNGG 2 cut(s) 416, 764
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 1 cut(s) 368
BseNI ACTGG 1 cut(s) 370
BseSI GKGCMC 1 cut(s) 759
BseXI GCAGC 3 cut(s) 259, 527, 553
BseYI CCCAGC 1 cut(s) 44
BsgI GTGCAG 1 cut(s) 384
Bsh1236I CGCG 2 cut(s) 192, 793
BshFI GGCC 4 cut(s) 107, 152, 173, 757
BshVI ATCGAT 2 cut(s) 231, 595
BsiHKAI GWGCWC 2 cut(s) 72, 81
BsiSI CCGG 2 cut(s) 104, 184
BslFI GGGAC 2 cut(s) 388, 696
BslI CCNNNNNNNGG 2 cut(s) 416, 764
BsmFI GGGAC 2 cut(s) 388, 696
BsnI GGCC 4 cut(s) 107, 152, 173, 757
Bsp120I GGGCCC 1 cut(s) 755
Bsp1286I GDGCHC 3 cut(s) 72, 81, 759
Bsp143I GATC 6 cut(s) 39, 60, 228, 394, 592, 808
Bsp19I CCATGG 1 cut(s) 153
Bsp68I TCGCGA 1 cut(s) 793
BspACI CCGC 2 cut(s) 8, 141
BspANI GGCC 4 cut(s) 107, 152, 173, 757
BspCNI CTCAG 1 cut(s) 367
BspDI ATCGAT 2 cut(s) 231, 595
BspFNI CGCG 2 cut(s) 192, 793
BspLI GGNNCC 4 cut(s) 376, 396, 684, 757
BspPI GGATC 4 cut(s) 68, 389, 402, 803
BspQI GCTCTTC 1 cut(s) 450
BsrDI GCAATG 1 cut(s) 636
BsrI ACTGG 1 cut(s) 370
BssECI CCNNGG 2 cut(s) 153, 609
BssMI GATC 6 cut(s) 39, 60, 228, 394, 592, 808
BssNI GRCGYC 2 cut(s) 23, 138
BssT1I CCWWGG 2 cut(s) 153, 609
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 1 cut(s) 56
Bst6I CTCTTC 1 cut(s) 450
BstACI GRCGYC 2 cut(s) 23, 138
BstAPI GCANNNNNTGC 1 cut(s) 539
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 1 cut(s) 354
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 3 cut(s) 207, 309, 686
BstFNI CGCG 2 cut(s) 192, 793
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 6 cut(s) 42, 63, 231, 397, 595, 811
BstMBI GATC 6 cut(s) 39, 60, 228, 394, 592, 808
BstMWI GCNNNNNNNGC 6 cut(s) 14, 76, 137, 149, 247, 539
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 2 cut(s) 100, 271
BstPAI GACNNNNGTC 1 cut(s) 131
BstSCI CCNGG 3 cut(s) 102, 168, 183
BstSLI GKGCMC 1 cut(s) 759
BstUI CGCG 2 cut(s) 192, 793
BstV1I GCAGC 3 cut(s) 259, 527, 553
BstX2I RGATCY 2 cut(s) 60, 394
BstXI CCANNNNNNTGG 1 cut(s) 438
BstYI RGATCY 2 cut(s) 60, 394
Bsu15I ATCGAT 2 cut(s) 231, 595
BsuI GTATCC 2 cut(s) 316, 796
BsuRI GGCC 4 cut(s) 107, 152, 173, 757
BsuTUI ATCGAT 2 cut(s) 231, 595
BtgI CCRYGG 1 cut(s) 153
BtgZI GCGATG 1 cut(s) 222
BtsCI GGATG 3 cut(s) 207, 309, 686
BtsIMutI CAGTG 2 cut(s) 117, 363
BtuMI TCGCGA 1 cut(s) 793
Cac8I GCNNGC 1 cut(s) 109
CfoI GCGC 1 cut(s) 283
Cfr13I GGNCC 6 cut(s) 166, 181, 375, 683, 755, 756
ClaI ATCGAT 2 cut(s) 231, 595
CseI GACGC 2 cut(s) 12, 146
Csp6I GTAC 2 cut(s) 100, 188
CviQI GTAC 2 cut(s) 100, 188
DdeI CTNAG 1 cut(s) 354
DpnI GATC 6 cut(s) 41, 62, 230, 396, 594, 810
DpnII GATC 6 cut(s) 39, 60, 228, 394, 592, 808
EaeI YGGCCR 1 cut(s) 105
Eam1104I CTCTTC 1 cut(s) 450
EarI CTCTTC 1 cut(s) 450
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 2 cut(s) 70, 79
Eco130I CCWWGG 2 cut(s) 153, 609
Eco24I GRGCYC 3 cut(s) 72, 81, 759
Eco47I GGWCC 4 cut(s) 166, 181, 375, 683
Eco53kI GAGCTC 2 cut(s) 70, 79
Eco57I CTGAAG 4 cut(s) 146, 263, 474, 564
EcoICRI GAGCTC 2 cut(s) 70, 79
EcoO109I RGGNCCY 1 cut(s) 375
EcoRI GAATTC 1 cut(s) 742
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 2 cut(s) 153, 609
EcoT22I ATGCAT 2 cut(s) 238, 636
EcoT38I GRGCYC 3 cut(s) 72, 81, 759
ErhI CCWWGG 2 cut(s) 153, 609
FaqI GGGAC 2 cut(s) 388, 696
FauNDI CATATG 1 cut(s) 538
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 4 cut(s) 141, 248, 516, 567
FokI GGATG 3 cut(s) 194, 316, 673
FriOI GRGCYC 3 cut(s) 72, 81, 759
Fsp4HI GCNGC 4 cut(s) 141, 248, 516, 567
FspBI CTAG 1 cut(s) 500
GlaI GCGC 1 cut(s) 282
GluI GCNGC 4 cut(s) 141, 248, 516, 567
GsaI CCCAGC 1 cut(s) 48
HaeIII GGCC 4 cut(s) 107, 152, 173, 757
HapII CCGG 2 cut(s) 104, 184
HgaI GACGC 2 cut(s) 12, 146
HhaI GCGC 1 cut(s) 283
Hin1I GRCGYC 2 cut(s) 23, 138
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HindIII AAGCTT 2 cut(s) 383, 695
HinfI GANTC 4 cut(s) 32, 114, 464, 795
HpaII CCGG 2 cut(s) 104, 184
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 2 cut(s) 60, 67
Hpy188III TCNNGA 5 cut(s) 296, 344, 665, 708, 792
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 4 cut(s) 28, 137, 140, 197
HpyAV CCTTC 5 cut(s) 142, 256, 388, 483, 673
HpyCH4III ACNGT 1 cut(s) 56
HpyCH4V TGCA 4 cut(s) 236, 401, 515, 634
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 76, 137, 149, 247, 539
HpyF3I CTNAG 1 cut(s) 354
Hsp92I GRCGYC 2 cut(s) 23, 138
HspAI GCGC 1 cut(s) 281
Kzo9I GATC 6 cut(s) 39, 60, 228, 394, 592, 808
LguI GCTCTTC 1 cut(s) 450
LmnI GCTCC 4 cut(s) 67, 75, 157, 574
Lsp1109I GCAGC 3 cut(s) 259, 527, 553
LweI GCATC 2 cut(s) 223, 245
MaeI CTAG 1 cut(s) 500
MaeIII GTNAC 2 cut(s) 115, 471
MalI GATC 6 cut(s) 41, 62, 230, 396, 594, 810
MboI GATC 6 cut(s) 39, 60, 228, 394, 592, 808
MboII GAAGA 3 cut(s) 467, 589, 738
MflI RGATCY 2 cut(s) 60, 394
MhlI GDGCHC 3 cut(s) 72, 81, 759
MluCI AATT 6 cut(s) 257, 273, 316, 550, 742, 761
MlyI GAGTC 3 cut(s) 26, 123, 804
MmeI TCCRAC 2 cut(s) 9, 38
MnlI CCTC 5 cut(s) 212, 385, 479, 799, 815
Mph1103I ATGCAT 2 cut(s) 238, 636
MseI TTAA 1 cut(s) 522
MslI CAYNNNNRTG 1 cut(s) 436
MspI CCGG 2 cut(s) 104, 184
MspR9I CCNGG 3 cut(s) 104, 170, 185
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 2 cut(s) 192, 793
MwoI GCNNNNNNNGC 6 cut(s) 14, 76, 137, 149, 247, 539
NciI CCSGG 2 cut(s) 104, 185
NcoI CCATGG 1 cut(s) 153
NdeI CATATG 1 cut(s) 538
NdeII GATC 6 cut(s) 39, 60, 228, 394, 592, 808
NlaIV GGNNCC 4 cut(s) 376, 396, 684, 757
NmuCI GTSAC 2 cut(s) 115, 471
NruI TCGCGA 1 cut(s) 793
NsiI ATGCAT 2 cut(s) 238, 636
NspI RCATGY 2 cut(s) 100, 271
OliI CACNNNNGTG 1 cut(s) 436
PciI ACATGT 2 cut(s) 96, 267
PciSI GCTCTTC 1 cut(s) 450
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PfeI GAWTC 1 cut(s) 464
PflFI GACNNNGTC 1 cut(s) 506
PkrI GCNGC 4 cut(s) 142, 249, 517, 568
PleI GAGTC 3 cut(s) 26, 122, 803
PpsI GAGTC 3 cut(s) 26, 122, 803
PpuMI RGGWCCY 1 cut(s) 375
PscI ACATGT 2 cut(s) 96, 267
PshAI GACNNNNGTC 1 cut(s) 131
PshBI ATTAAT 1 cut(s) 522
PsiI TTATAA 1 cut(s) 446
Psp124BI GAGCTC 2 cut(s) 72, 81
Psp5II RGGWCCY 1 cut(s) 375
Psp6I CCWGG 1 cut(s) 168
PspFI CCCAGC 1 cut(s) 44
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 4 cut(s) 376, 396, 684, 757
PspOMI GGGCCC 1 cut(s) 755
PspPI GGNCC 6 cut(s) 166, 181, 375, 683, 755, 756
PspPPI RGGWCCY 1 cut(s) 375
PsuI RGATCY 2 cut(s) 60, 394
PsyI GACNNNGTC 1 cut(s) 506
RruI TCGCGA 1 cut(s) 793
RsaI GTAC 2 cut(s) 101, 189
RsaNI GTAC 2 cut(s) 100, 188
RseI CAYNNNNRTG 1 cut(s) 436
SacI GAGCTC 2 cut(s) 72, 81
SalI GTCGAC 1 cut(s) 134
SapI GCTCTTC 1 cut(s) 450
SaqAI TTAA 1 cut(s) 522
SatI GCNGC 4 cut(s) 141, 248, 516, 567
Sau3AI GATC 6 cut(s) 39, 60, 228, 394, 592, 808
Sau96I GGNCC 6 cut(s) 166, 181, 375, 683, 755, 756
SchI GAGTC 3 cut(s) 26, 123, 804
ScrFI CCNGG 3 cut(s) 104, 170, 185
SduI GDGCHC 3 cut(s) 72, 81, 759
SfaNI GCATC 2 cut(s) 223, 245
SgrDI CGTCGACG 1 cut(s) 134
SinI GGWCC 4 cut(s) 166, 181, 375, 683
SmiMI CAYNNNNRTG 1 cut(s) 436
Sse9I AATT 6 cut(s) 257, 273, 316, 550, 742, 761
SsiI CCGC 2 cut(s) 8, 141
SspMI CTAG 1 cut(s) 500
SstI GAGCTC 2 cut(s) 72, 81
StyD4I CCNGG 3 cut(s) 102, 168, 183
StyI CCWWGG 2 cut(s) 153, 609
TaaI ACNGT 1 cut(s) 56
TaqI TCGA 6 cut(s) 135, 231, 595, 666, 740, 807
TasI AATT 6 cut(s) 257, 273, 316, 550, 742, 761
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 1 cut(s) 464
Tru1I TTAA 1 cut(s) 522
Tru9I TTAA 1 cut(s) 522
TscAI CASTG 2 cut(s) 124, 370
TseFI GTSAC 2 cut(s) 115, 471
TseI GCWGC 3 cut(s) 247, 515, 566
Tsp45I GTSAC 2 cut(s) 115, 471
TspDTI ATGAA 2 cut(s) 651, 692
TspRI CASTG 2 cut(s) 124, 370
Tth111I GACNNNGTC 1 cut(s) 506
VpaK11BI GGWCC 4 cut(s) 166, 181, 375, 683
VspI ATTAAT 1 cut(s) 522
XapI RAATTY 3 cut(s) 316, 742, 761
XceI RCATGY 2 cut(s) 100, 271
XmiI GTMKAC 1 cut(s) 135
XspI CTAG 1 cut(s) 500
Zsp2I ATGCAT 2 cut(s) 238, 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.