RLG00000028581

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001324
Physical Location & Seq
Reverse (-)
24608501 .. 24622276
13776 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028581

Sequence Viewer

Length: 1158 bp
ATGGACTACATCCAGATCGACGCCGATGAAGTCCCGATGACTCTTGAGAATACCCAAACTCAATATTCCGATTCCGATTGCGATAGCCTCCATTCCGAATCGAATTCCTGTTCTTCTATTGAAATTGAAGTTGATCAGGATTCCGAGTCCTATTGTGAATCGGAGTCGCCAGTTTCTGATCCATGTGGGGCTTTCACTGCAGATGAAATGATGACTAGAGTGGAGGGTATGGAGCGTGAGACTATTACGAAGAGCTTTGTTTCCGTTATGAAAGCCACCGACGGAGAAACCAGAGTGGTGGCTATGCATAAGAATTCGATGTACCCGGCCAGCAGCATCACCGATTTGACGGCTGTCGCCGCCGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGCCATCAAGGACTGGAAACTGATCGATGCATCCCTGAAGCAGCAAAGGAAATTGAAGGATATGTCAATTTATGACCCCGCTCATGTTCCTGAAAGAATGTTGATGTTGAATTTGGATACCAATAGATGTTTATTTCCAGAAAGCTCTCAACAAAACACTGGTTCTGGGACCTTCAAGCTTGAGGAGGATCCTGCACCACTACCACCACCACCCAAGGAGAAAAAGGGTCGTTCCACGCCACCCTTGTGGTTTTTGAGTGCTGAATATATTACTAATTGCAGATACATGAGAGGAAGGCCTACACTAGAAAAGGTCAAGGCAGCTATTAATGACATGGCAACATATGCTGAAGCAAATTATCAGCTCATAGCTGCTCCAAAGAAGAATGTGGCAGGAGATCGTTGGGAAAAGGCCTTGCACAGCTCCTACACTGGTTACATGTCAACAATCATGCAGGTGACAGGAGATCGTTGCCAAAACGCGGGGATTAAAATACCTAATGGACGCAGTATAACGATTAATACACATTCCAAGGAAATTAATCAAAATCAAAAGATATCATTGCAAAATGAAAATACGGAAAGTAAGGAAGAATACCATGCACAAGACGATCAGCTAGGTGTTTCGCTTCTACTGACCAACTCTGGTGCCGGAGACACCATTCCCTTTCCCTTTCTGCCTCTTTCTCCAAGTCATCGAGAAGTACTCACTGATTGTCATCGATCTGCAGCTGTCAGTATTAATTTCGGGAGAAGGCGTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

386

Amino Acids

42.85

Weight (kDa)

5.13

Isoelectric Point (pI)

49.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 102 - 270 6.7e-26 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 838
Acc36I ACCTGC 1 cut(s) 838
AccB1I GGYRCC 1 cut(s) 1040
AccBSI CCGCTC 1 cut(s) 473
AccII CGCG 1 cut(s) 875
AciI CCGC 4 cut(s) 360, 363, 471, 875
AclWI GGATC 3 cut(s) 173, 575, 588
AcoI YGGCCR 1 cut(s) 327
AcsI RAATTY 3 cut(s) 103, 313, 502
AcuI CTGAAG 3 cut(s) 368, 449, 762
AcyI GRCGYC 1 cut(s) 21
AfaI GTAC 2 cut(s) 323, 1098
AfiI CCNNNNNNNGG 1 cut(s) 874
AflIII ACRYGT 1 cut(s) 831
AgsI TTSAA 5 cut(s) 122, 128, 448, 502, 568
AjnI CCWGG 1 cut(s) 390
AleI CACNNNNGTG 1 cut(s) 637
Alw26I GTCTC 2 cut(s) 233, 1041
AlwI GGATC 3 cut(s) 173, 575, 588
AlwNI CAGNNNCTG 1 cut(s) 176
AoxI GGCC 5 cut(s) 327, 372, 393, 689, 804
ApeKI GCWGC 5 cut(s) 333, 433, 713, 764, 1121
ApoI RAATTY 3 cut(s) 103, 313, 502
AseI ATTAAT 4 cut(s) 720, 912, 933, 1134
AspS9I GGNCC 2 cut(s) 388, 561
AsuC2I CCSGG 1 cut(s) 326
AsuHPI GGTGA 2 cut(s) 331, 862
AvaII GGWCC 2 cut(s) 388, 561
BamHI GGATCC 1 cut(s) 580
BanI GGYRCC 1 cut(s) 1040
BbvI GCAGC 5 cut(s) 345, 445, 725, 751, 1133
BccI CCATC 1 cut(s) 404
BceAI ACGGC 2 cut(s) 350, 366
BciT130I CCWGG 1 cut(s) 392
BciVI GTATCC 1 cut(s) 502
BclI TGATCA 1 cut(s) 133
BcnI CCSGG 1 cut(s) 326
BcoDI GTCTC 2 cut(s) 233, 1041
BfaI CTAG 3 cut(s) 216, 698, 1010
BfmI CTRYAG 2 cut(s) 198, 1119
BfuAI ACCTGC 1 cut(s) 838
BfuI GTATCC 1 cut(s) 502
BglI GCCNNNNNGGC 1 cut(s) 371
BisI GCNGC 7 cut(s) 334, 360, 363, 434, 714, 765, 1122
BlsI GCNGC 7 cut(s) 335, 361, 364, 435, 715, 766, 1123
BmcAI AGTACT 1 cut(s) 1098
Bme1390I CCNGG 2 cut(s) 326, 392
Bme18I GGWCC 2 cut(s) 388, 561
BmgT120I GGNCC 2 cut(s) 388, 561
BmiI GGNNCC 3 cut(s) 562, 582, 1042
BmrFI CCNGG 2 cut(s) 326, 392
BmsI GCATC 3 cut(s) 345, 409, 431
BoxI GACNNNNGTC 1 cut(s) 353
BplI GAGNNNNNCTC 2 cut(s) 1083, 1115
BpuEI CTTGAG 2 cut(s) 65, 593
BpuMI CCSGG 1 cut(s) 326
Bsa29I ATCGAT 2 cut(s) 417, 1114
BsaBI GATNNNNATC 1 cut(s) 1110
BsaHI GRCGYC 1 cut(s) 21
BsaJI CCNNGG 3 cut(s) 375, 606, 924
BsaXI ACNNNNNCTCC 1 cut(s) 1135
Bsc4I CCNNNNNNNGG 1 cut(s) 874
Bse1I ACTGG 4 cut(s) 170, 410, 556, 829
Bse3DI GCAATG 1 cut(s) 953
Bse8I GATNNNNATC 1 cut(s) 1110
BseBI CCWGG 1 cut(s) 392
BseCI ATCGAT 2 cut(s) 417, 1114
BseDI CCNNGG 3 cut(s) 375, 606, 924
BseGI GGATG 2 cut(s) 9, 422
BseJI GATNNNNATC 1 cut(s) 1110
BseLI CCNNNNNNNGG 1 cut(s) 874
BseMI GCAATG 1 cut(s) 953
BseNI ACTGG 4 cut(s) 170, 410, 556, 829
BseRI GAGGAG 1 cut(s) 590
BseXI GCAGC 5 cut(s) 345, 445, 725, 751, 1133
BsgI GTGCAG 1 cut(s) 570
Bsh1236I CGCG 1 cut(s) 875
BshFI GGCC 5 cut(s) 329, 374, 395, 691, 806
BshNI GGYRCC 1 cut(s) 1040
BshVI ATCGAT 2 cut(s) 417, 1114
BsiSI CCGG 2 cut(s) 326, 1044
BslFI GGGAC 2 cut(s) 17, 574
BslI CCNNNNNNNGG 1 cut(s) 874
BsmAI GTCTC 2 cut(s) 233, 1041
BsmFI GGGAC 2 cut(s) 17, 574
BsnI GGCC 5 cut(s) 329, 374, 395, 691, 806
Bsp143I GATC 9 cut(s) 15, 133, 178, 414, 580, 790, 859, 1003, 1115
Bsp19I CCATGG 1 cut(s) 375
BspACI CCGC 4 cut(s) 360, 363, 471, 875
BspANI GGCC 5 cut(s) 329, 374, 395, 691, 806
BspDI ATCGAT 2 cut(s) 417, 1114
BspFNI CGCG 1 cut(s) 875
BspLI GGNNCC 3 cut(s) 562, 582, 1042
BspMAI CTGCAG 2 cut(s) 202, 1123
BspMI ACCTGC 1 cut(s) 838
BspPI GGATC 3 cut(s) 173, 575, 588
BspQI GCTCTTC 1 cut(s) 245
BspT107I GGYRCC 1 cut(s) 1040
BsrBI CCGCTC 1 cut(s) 473
BsrDI GCAATG 1 cut(s) 953
BsrI ACTGG 4 cut(s) 170, 410, 556, 829
BssECI CCNNGG 3 cut(s) 375, 606, 924
BssMI GATC 9 cut(s) 15, 133, 178, 414, 580, 790, 859, 1003, 1115
BssNI GRCGYC 1 cut(s) 21
BssT1I CCWWGG 3 cut(s) 375, 606, 924
Bst2UI CCWGG 1 cut(s) 392
Bst6I CTCTTC 1 cut(s) 245
BstACI GRCGYC 1 cut(s) 21
BstAPI GCANNNNNTGC 1 cut(s) 737
BstC8I GCNNGC 2 cut(s) 331, 1152
BstDEI CTNAG 1 cut(s) 1155
BstDSI CCRYGG 1 cut(s) 375
BstF5I GGATG 2 cut(s) 9, 422
BstFNI CGCG 1 cut(s) 875
BstKTI GATC 9 cut(s) 18, 136, 181, 417, 583, 793, 862, 1006, 1118
BstMAI GTCTC 2 cut(s) 233, 1041
BstMBI GATC 9 cut(s) 15, 133, 178, 414, 580, 790, 859, 1003, 1115
BstMWI GCNNNNNNNGC 4 cut(s) 197, 359, 371, 737
BstNI CCWGG 1 cut(s) 392
BstNSI RCATGY 1 cut(s) 835
BstPAI GACNNNNGTC 1 cut(s) 353
BstSCI CCNGG 2 cut(s) 324, 390
BstSFI CTRYAG 2 cut(s) 198, 1119
BstUI CGCG 1 cut(s) 875
BstV1I GCAGC 5 cut(s) 345, 445, 725, 751, 1133
BstX2I RGATCY 1 cut(s) 580
BstXI CCANNNNNNTGG 2 cut(s) 298, 639
BstYI RGATCY 1 cut(s) 580
Bsu15I ATCGAT 2 cut(s) 417, 1114
BsuI GTATCC 1 cut(s) 502
BsuRI GGCC 5 cut(s) 329, 374, 395, 691, 806
BsuTUI ATCGAT 2 cut(s) 417, 1114
BtgI CCRYGG 1 cut(s) 375
BtsCI GGATG 2 cut(s) 9, 422
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 4 cut(s) 195, 549, 822, 1101
BveI ACCTGC 1 cut(s) 838
Cac8I GCNNGC 2 cut(s) 331, 1152
CaiI CAGNNNCTG 1 cut(s) 176
Cfr13I GGNCC 2 cut(s) 388, 561
ClaI ATCGAT 2 cut(s) 417, 1114
CseI GACGC 2 cut(s) 29, 906
Csp6I GTAC 2 cut(s) 322, 1097
CviAII CATG 8 cut(s) 183, 376, 476, 679, 727, 832, 844, 992
CviQI GTAC 2 cut(s) 322, 1097
DdeI CTNAG 1 cut(s) 1155
DpnI GATC 9 cut(s) 17, 135, 180, 416, 582, 792, 861, 1005, 1117
DpnII GATC 9 cut(s) 15, 133, 178, 414, 580, 790, 859, 1003, 1115
EaeI YGGCCR 1 cut(s) 327
Eam1104I CTCTTC 1 cut(s) 245
EarI CTCTTC 1 cut(s) 245
Eco130I CCWWGG 3 cut(s) 375, 606, 924
Eco147I AGGCCT 2 cut(s) 691, 806
Eco32I GATATC 1 cut(s) 951
Eco47I GGWCC 2 cut(s) 388, 561
Eco57I CTGAAG 3 cut(s) 368, 449, 762
EcoO109I RGGNCCY 1 cut(s) 561
EcoRI GAATTC 2 cut(s) 103, 313
EcoRII CCWGG 1 cut(s) 390
EcoRV GATATC 1 cut(s) 951
EcoT14I CCWWGG 3 cut(s) 375, 606, 924
EcoT22I ATGCAT 2 cut(s) 309, 424
ErhI CCWWGG 3 cut(s) 375, 606, 924
FaeI CATG 8 cut(s) 186, 379, 479, 682, 730, 835, 847, 995
FalI AAGNNNNNCTT 1 cut(s) 1138
FaqI GGGAC 2 cut(s) 17, 574
FatI CATG 8 cut(s) 182, 375, 475, 678, 726, 831, 843, 991
FauI CCCGC 2 cut(s) 478, 868
FauNDI CATATG 1 cut(s) 736
FbaI TGATCA 1 cut(s) 133
Fnu4HI GCNGC 7 cut(s) 334, 360, 363, 434, 714, 765, 1122
FokI GGATG 1 cut(s) 409
Fsp4HI GCNGC 7 cut(s) 334, 360, 363, 434, 714, 765, 1122
FspBI CTAG 3 cut(s) 216, 698, 1010
GluI GCNGC 7 cut(s) 334, 360, 363, 434, 714, 765, 1122
HaeIII GGCC 5 cut(s) 329, 374, 395, 691, 806
HapII CCGG 2 cut(s) 326, 1044
HgaI GACGC 2 cut(s) 29, 906
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 8 cut(s) 186, 379, 479, 682, 730, 835, 847, 995
HincII GTYRAC 1 cut(s) 837
HindII GTYRAC 1 cut(s) 837
HindIII AAGCTT 1 cut(s) 569
HinfI GANTC 7 cut(s) 40, 71, 98, 140, 146, 158, 164
HpaII CCGG 2 cut(s) 326, 1044
HphI GGTGA 2 cut(s) 331, 862
Hpy166II GTNNAC 1 cut(s) 837
Hpy188I TCNGA 6 cut(s) 70, 76, 97, 145, 163, 178
Hpy188III TCNNGA 8 cut(s) 13, 34, 44, 137, 482, 530, 1091, 1141
Hpy8I GTNNAC 1 cut(s) 837
Hpy99I CGWCG 2 cut(s) 23, 284
HpyAV CCTTC 5 cut(s) 364, 442, 574, 681, 1140
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 359, 371, 737
HpyF3I CTNAG 1 cut(s) 1155
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 8 cut(s) 186, 379, 479, 682, 730, 835, 847, 995
Ksp22I TGATCA 1 cut(s) 133
Kzo9I GATC 9 cut(s) 15, 133, 178, 414, 580, 790, 859, 1003, 1115
LguI GCTCTTC 1 cut(s) 245
LmnI GCTCC 4 cut(s) 232, 379, 772, 821
Lsp1109I GCAGC 5 cut(s) 345, 445, 725, 751, 1133
LweI GCATC 3 cut(s) 345, 409, 431
MaeI CTAG 3 cut(s) 216, 698, 1010
MaeIII GTNAC 2 cut(s) 827, 850
MalI GATC 9 cut(s) 17, 135, 180, 416, 582, 792, 861, 1005, 1117
MbiI CCGCTC 1 cut(s) 473
MboI GATC 9 cut(s) 15, 133, 178, 414, 580, 790, 859, 1003, 1115
MboII GAAGA 4 cut(s) 105, 262, 787, 995
MflI RGATCY 1 cut(s) 580
MlyI GAGTC 3 cut(s) 34, 155, 173
MnlI CCTC 6 cut(s) 98, 217, 568, 571, 677, 1083
Mph1103I ATGCAT 2 cut(s) 309, 424
MseI TTAA 5 cut(s) 720, 882, 912, 933, 1134
MslI CAYNNNNRTG 2 cut(s) 637, 848
MspA1I CMGCKG 1 cut(s) 1124
MspI CCGG 2 cut(s) 326, 1044
MspR9I CCNGG 2 cut(s) 326, 392
MvaI CCWGG 1 cut(s) 392
MvnI CGCG 1 cut(s) 875
MwoI GCNNNNNNNGC 4 cut(s) 197, 359, 371, 737
NciI CCSGG 1 cut(s) 326
NcoI CCATGG 1 cut(s) 375
NdeI CATATG 1 cut(s) 736
NdeII GATC 9 cut(s) 15, 133, 178, 414, 580, 790, 859, 1003, 1115
NlaIII CATG 8 cut(s) 186, 379, 479, 682, 730, 835, 847, 995
NlaIV GGNNCC 3 cut(s) 562, 582, 1042
NmuCI GTSAC 1 cut(s) 850
NsiI ATGCAT 2 cut(s) 309, 424
NspI RCATGY 1 cut(s) 835
OliI CACNNNNGTG 1 cut(s) 637
PaqCI CACCTGC 1 cut(s) 838
PceI AGGCCT 2 cut(s) 691, 806
PciI ACATGT 1 cut(s) 831
PciSI GCTCTTC 1 cut(s) 245
PcsI WCGNNNNNNNCGW 1 cut(s) 363
PfeI GAWTC 4 cut(s) 71, 98, 140, 158
PkrI GCNGC 7 cut(s) 335, 361, 364, 435, 715, 766, 1123
PleI GAGTC 3 cut(s) 34, 154, 172
PpsI GAGTC 3 cut(s) 34, 154, 172
PpuMI RGGWCCY 1 cut(s) 561
PscI ACATGT 1 cut(s) 831
PshAI GACNNNNGTC 1 cut(s) 353
PshBI ATTAAT 4 cut(s) 720, 912, 933, 1134
Psp5II RGGWCCY 1 cut(s) 561
Psp6I CCWGG 1 cut(s) 390
PspGI CCWGG 1 cut(s) 390
PspN4I GGNNCC 3 cut(s) 562, 582, 1042
PspPI GGNCC 2 cut(s) 388, 561
PspPPI RGGWCCY 1 cut(s) 561
PstI CTGCAG 2 cut(s) 202, 1123
PstNI CAGNNNCTG 1 cut(s) 176
PsuI RGATCY 1 cut(s) 580
PvuII CAGCTG 1 cut(s) 1124
RsaI GTAC 2 cut(s) 323, 1098
RsaNI GTAC 2 cut(s) 322, 1097
RseI CAYNNNNRTG 2 cut(s) 637, 848
SapI GCTCTTC 1 cut(s) 245
SaqAI TTAA 5 cut(s) 720, 882, 912, 933, 1134
SatI GCNGC 7 cut(s) 334, 360, 363, 434, 714, 765, 1122
Sau3AI GATC 9 cut(s) 15, 133, 178, 414, 580, 790, 859, 1003, 1115
Sau96I GGNCC 2 cut(s) 388, 561
ScaI AGTACT 1 cut(s) 1098
SchI GAGTC 3 cut(s) 34, 155, 173
ScrFI CCNGG 2 cut(s) 326, 392
SfaNI GCATC 3 cut(s) 345, 409, 431
SfcI CTRYAG 2 cut(s) 198, 1119
SinI GGWCC 2 cut(s) 388, 561
SmiMI CAYNNNNRTG 2 cut(s) 637, 848
SmlI CTYRAG 2 cut(s) 44, 572
SmoI CTYRAG 2 cut(s) 44, 572
SseBI AGGCCT 2 cut(s) 691, 806
SsiI CCGC 4 cut(s) 360, 363, 471, 875
SspI AATATT 1 cut(s) 65
SspMI CTAG 3 cut(s) 216, 698, 1010
StuI AGGCCT 2 cut(s) 691, 806
StyD4I CCNGG 2 cut(s) 324, 390
StyI CCWWGG 3 cut(s) 375, 606, 924
TaqI TCGA 6 cut(s) 18, 101, 317, 417, 1090, 1114
TatI WGTACW 1 cut(s) 1096
TauI GCSGC 2 cut(s) 362, 365
TfiI GAWTC 4 cut(s) 71, 98, 140, 158
Tru1I TTAA 5 cut(s) 720, 882, 912, 933, 1134
Tru9I TTAA 5 cut(s) 720, 882, 912, 933, 1134
TscAI CASTG 4 cut(s) 202, 556, 829, 1108
TseFI GTSAC 1 cut(s) 850
TseI GCWGC 5 cut(s) 333, 433, 713, 764, 1121
Tsp45I GTSAC 1 cut(s) 850
TspDTI ATGAA 4 cut(s) 42, 219, 284, 978
TspGWI ACGGA 3 cut(s) 253, 297, 986
TspRI CASTG 4 cut(s) 202, 556, 829, 1108
VpaK11BI GGWCC 2 cut(s) 388, 561
VspI ATTAAT 4 cut(s) 720, 912, 933, 1134
XapI RAATTY 3 cut(s) 103, 313, 502
XceI RCATGY 1 cut(s) 835
XspI CTAG 3 cut(s) 216, 698, 1010
ZrmI AGTACT 1 cut(s) 1098
Zsp2I ATGCAT 2 cut(s) 309, 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.