RLG00000028583

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001324
Physical Location & Seq
Reverse (-)
24638026 .. 24639925
1900 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028583

Sequence Viewer

Length: 549 bp
ATGGCCGTCGACGCCGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGCCATCAAGGACCTGGTTCGCGAAGAAACCCTCGCCATCCCCAAAGCAAAAAAACTGATCAATGCATCGCTGAAGCAGCAAAGGAAATTGAAGGACATGTCAATCACTCATGATCCTGAAAGGATGTCCATGTTGAATTTGGATACAAATAGATGTTTATTTCCAGATAGCTCTCAGCAAAACACTGGTTCTGGGACCTTCAAGCTTGATGAGGATCCTGCACCACCACCAGCACCGCCTAAGGTACATCTCTCTCTCTCTCTCTCTCACACACACACACACACACTCACACTTCTGGCCACTATTCTTTCTGTTTTTGTTAATTCCACGTCACCATTGTGGTTTATAAGTGCTGAAGAGCTGGATTCCTTGTCACCATACATGAGAGGAAGGCTTACACTAGAGAAGGTCAATGCAGCTATTAGTGACATGGCAACATATGCTGAAGCAAATTATCAGCCCGTAGCTGCTCCACAGAAGAATGTTTGTGCTCTGCCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

183

Amino Acids

19.98

Weight (kDa)

6.51

Isoelectric Point (pI)

45.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 2 - 176 9.3e-30 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 392
AccI GTMKAC 1 cut(s) 9
AccII CGCG 1 cut(s) 66
AciI CCGC 2 cut(s) 15, 281
AclWI GGATC 3 cut(s) 152, 254, 267
AcoI YGGCCR 2 cut(s) 3, 342
AcsI RAATTY 1 cut(s) 181
AcuI CTGAAG 4 cut(s) 20, 137, 420, 510
AcyI GRCGYC 1 cut(s) 12
AfaI GTAC 1 cut(s) 291
AflIII ACRYGT 1 cut(s) 141
AgsI TTSAA 3 cut(s) 136, 181, 247
AjiI CACGTC 1 cut(s) 375
AjnI CCWGG 2 cut(s) 42, 57
AleI CACNNNNGTG 1 cut(s) 382
AluBI AGCT 6 cut(s) 34, 216, 250, 406, 464, 512
AluI AGCT 6 cut(s) 34, 216, 250, 406, 464, 512
Alw21I GWGCWC 1 cut(s) 538
AlwI GGATC 3 cut(s) 152, 254, 267
AoxI GGCC 4 cut(s) 3, 24, 45, 342
ApeKI GCWGC 3 cut(s) 121, 461, 512
ApoI RAATTY 1 cut(s) 181
AspS9I GGNCC 3 cut(s) 40, 55, 240
AsuHPI GGTGA 2 cut(s) 369, 411
AvaII GGWCC 3 cut(s) 40, 55, 240
AxyI CCTNAGG 1 cut(s) 285
BalI TGGCCA 1 cut(s) 344
BamHI GGATCC 1 cut(s) 259
Bbv12I GWGCWC 1 cut(s) 538
BbvI GCAGC 3 cut(s) 133, 473, 499
BccI CCATC 2 cut(s) 56, 89
BceAI ACGGC 1 cut(s) 2
BciT130I CCWGG 2 cut(s) 44, 59
BciVI GTATCC 1 cut(s) 181
BclI TGATCA 1 cut(s) 102
BfaI CTAG 1 cut(s) 446
BfuI GTATCC 1 cut(s) 181
BglI GCCNNNNNGGC 1 cut(s) 23
BisI GCNGC 4 cut(s) 15, 122, 462, 513
BlsI GCNGC 4 cut(s) 16, 123, 463, 514
Bme1390I CCNGG 2 cut(s) 44, 59
Bme18I GGWCC 3 cut(s) 40, 55, 240
BmgBI CACGTC 1 cut(s) 375
BmgT120I GGNCC 3 cut(s) 40, 55, 240
BmiI GGNNCC 2 cut(s) 241, 261
BmrFI CCNGG 2 cut(s) 44, 59
BmsI GCATC 1 cut(s) 119
BsaBI GATNNNNATC 1 cut(s) 258
BsaHI GRCGYC 1 cut(s) 12
BsaJI CCNNGG 1 cut(s) 27
Bse1I ACTGG 1 cut(s) 235
Bse21I CCTNAGG 1 cut(s) 285
Bse8I GATNNNNATC 1 cut(s) 258
BseBI CCWGG 2 cut(s) 44, 59
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 2 cut(s) 81, 174
BseJI GATNNNNATC 1 cut(s) 258
BseMII CTCAG 1 cut(s) 233
BseNI ACTGG 1 cut(s) 235
BseXI GCAGC 3 cut(s) 133, 473, 499
BsgI GTGCAG 1 cut(s) 249
Bsh1236I CGCG 1 cut(s) 66
BshFI GGCC 4 cut(s) 5, 26, 47, 344
BsiHKAI GWGCWC 1 cut(s) 538
BslFI GGGAC 1 cut(s) 253
BsmFI GGGAC 1 cut(s) 253
BsnI GGCC 4 cut(s) 5, 26, 47, 344
Bsp1286I GDGCHC 1 cut(s) 538
Bsp143I GATC 3 cut(s) 102, 157, 259
Bsp19I CCATGG 1 cut(s) 27
Bsp68I TCGCGA 1 cut(s) 66
BspACI CCGC 2 cut(s) 15, 281
BspANI GGCC 4 cut(s) 5, 26, 47, 344
BspCNI CTCAG 1 cut(s) 232
BspFNI CGCG 1 cut(s) 66
BspHI TCATGA 1 cut(s) 154
BspLI GGNNCC 2 cut(s) 241, 261
BspPI GGATC 3 cut(s) 152, 254, 267
BspQI GCTCTTC 1 cut(s) 396
BsrI ACTGG 1 cut(s) 235
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 3 cut(s) 102, 157, 259
BssNI GRCGYC 1 cut(s) 12
BssT1I CCWWGG 1 cut(s) 27
Bst2UI CCWGG 2 cut(s) 44, 59
Bst6I CTCTTC 1 cut(s) 396
BstACI GRCGYC 1 cut(s) 12
BstAPI GCANNNNNTGC 1 cut(s) 485
BstDEI CTNAG 2 cut(s) 219, 285
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 2 cut(s) 81, 174
BstFNI CGCG 1 cut(s) 66
BstKTI GATC 3 cut(s) 105, 160, 262
BstMBI GATC 3 cut(s) 102, 157, 259
BstMWI GCNNNNNNNGC 4 cut(s) 11, 23, 121, 485
BstNI CCWGG 2 cut(s) 44, 59
BstNSI RCATGY 1 cut(s) 145
BstSCI CCNGG 2 cut(s) 42, 57
BstUI CGCG 1 cut(s) 66
BstV1I GCAGC 3 cut(s) 133, 473, 499
BstX2I RGATCY 1 cut(s) 259
BstYI RGATCY 1 cut(s) 259
Bsu36I CCTNAGG 1 cut(s) 285
BsuI GTATCC 1 cut(s) 181
BsuRI GGCC 4 cut(s) 5, 26, 47, 344
BtgI CCRYGG 1 cut(s) 27
BtgZI GCGATG 1 cut(s) 96
BtrI CACGTC 1 cut(s) 375
BtsCI GGATG 2 cut(s) 81, 174
BtsIMutI CAGTG 1 cut(s) 228
BtuMI TCGCGA 1 cut(s) 66
CciI TCATGA 1 cut(s) 154
Cfr13I GGNCC 3 cut(s) 40, 55, 240
CseI GACGC 1 cut(s) 20
CsiI ACCWGGT 1 cut(s) 57
Csp6I GTAC 1 cut(s) 290
CviAII CATG 6 cut(s) 28, 142, 155, 175, 427, 475
CviQI GTAC 1 cut(s) 290
DdeI CTNAG 2 cut(s) 219, 285
DpnI GATC 3 cut(s) 104, 159, 261
DpnII GATC 3 cut(s) 102, 157, 259
EaeI YGGCCR 2 cut(s) 3, 342
Eam1104I CTCTTC 1 cut(s) 396
EarI CTCTTC 1 cut(s) 396
Eco130I CCWWGG 1 cut(s) 27
Eco47I GGWCC 3 cut(s) 40, 55, 240
Eco57I CTGAAG 4 cut(s) 20, 137, 420, 510
Eco81I CCTNAGG 1 cut(s) 285
EcoO109I RGGNCCY 2 cut(s) 55, 240
EcoRII CCWGG 2 cut(s) 42, 57
EcoT14I CCWWGG 1 cut(s) 27
EcoT22I ATGCAT 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 6 cut(s) 31, 145, 158, 178, 430, 478
FaqI GGGAC 1 cut(s) 253
FatI CATG 6 cut(s) 27, 141, 154, 174, 426, 474
FauNDI CATATG 1 cut(s) 484
FbaI TGATCA 1 cut(s) 102
FblI GTMKAC 1 cut(s) 9
Fnu4HI GCNGC 4 cut(s) 15, 122, 462, 513
FokI GGATG 2 cut(s) 68, 181
Fsp4HI GCNGC 4 cut(s) 15, 122, 462, 513
FspBI CTAG 1 cut(s) 446
GluI GCNGC 4 cut(s) 15, 122, 462, 513
HaeIII GGCC 4 cut(s) 5, 26, 47, 344
HgaI GACGC 1 cut(s) 20
Hin1I GRCGYC 1 cut(s) 12
Hin1II CATG 6 cut(s) 31, 145, 158, 178, 430, 478
HincII GTYRAC 1 cut(s) 10
HindII GTYRAC 1 cut(s) 10
HindIII AAGCTT 1 cut(s) 248
HinfI GANTC 1 cut(s) 410
HphI GGTGA 2 cut(s) 369, 411
Hpy166II GTNNAC 1 cut(s) 10
Hpy188I TCNGA 1 cut(s) 548
Hpy188III TCNNGA 4 cut(s) 65, 155, 161, 209
Hpy8I GTNNAC 1 cut(s) 10
Hpy99I CGWCG 2 cut(s) 11, 14
HpyAV CCTTC 5 cut(s) 16, 130, 253, 429, 445
HpyCH4IV ACGT 1 cut(s) 374
HpyCH4V TGCA 3 cut(s) 110, 266, 461
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 23, 121, 485
HpyF3I CTNAG 2 cut(s) 219, 285
HpySE526I ACGT 1 cut(s) 374
Hsp92I GRCGYC 1 cut(s) 12
Hsp92II CATG 6 cut(s) 31, 145, 158, 178, 430, 478
Ksp22I TGATCA 1 cut(s) 102
Kzo9I GATC 3 cut(s) 102, 157, 259
LguI GCTCTTC 1 cut(s) 396
LmnI GCTCC 2 cut(s) 31, 520
Lsp1109I GCAGC 3 cut(s) 133, 473, 499
LweI GCATC 1 cut(s) 119
MabI ACCWGGT 1 cut(s) 57
MaeI CTAG 1 cut(s) 446
MaeII ACGT 1 cut(s) 374
MaeIII GTNAC 3 cut(s) 375, 417, 470
MalI GATC 3 cut(s) 104, 159, 261
MboI GATC 3 cut(s) 102, 157, 259
MboII GAAGA 3 cut(s) 80, 413, 535
MflI RGATCY 1 cut(s) 259
MhlI GDGCHC 1 cut(s) 538
MlsI TGGCCA 1 cut(s) 344
MluCI AATT 4 cut(s) 131, 181, 367, 496
MluNI TGGCCA 1 cut(s) 344
MnlI CCTC 3 cut(s) 86, 250, 425
Mox20I TGGCCA 1 cut(s) 344
Mph1103I ATGCAT 1 cut(s) 112
MscI TGGCCA 1 cut(s) 344
MseI TTAA 1 cut(s) 366
MslI CAYNNNNRTG 1 cut(s) 382
Msp20I TGGCCA 1 cut(s) 344
MspR9I CCNGG 2 cut(s) 44, 59
MvaI CCWGG 2 cut(s) 44, 59
MvnI CGCG 1 cut(s) 66
MwoI GCNNNNNNNGC 4 cut(s) 11, 23, 121, 485
NcoI CCATGG 1 cut(s) 27
NdeI CATATG 1 cut(s) 484
NdeII GATC 3 cut(s) 102, 157, 259
NlaIII CATG 6 cut(s) 31, 145, 158, 178, 430, 478
NlaIV GGNNCC 2 cut(s) 241, 261
NmuCI GTSAC 3 cut(s) 375, 417, 470
NruI TCGCGA 1 cut(s) 66
NsiI ATGCAT 1 cut(s) 112
NspI RCATGY 1 cut(s) 145
OliI CACNNNNGTG 1 cut(s) 382
PagI TCATGA 1 cut(s) 154
PciI ACATGT 1 cut(s) 141
PciSI GCTCTTC 1 cut(s) 396
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PfeI GAWTC 1 cut(s) 410
PkrI GCNGC 4 cut(s) 16, 123, 463, 514
PpuMI RGGWCCY 2 cut(s) 55, 240
PscI ACATGT 1 cut(s) 141
PsiI TTATAA 1 cut(s) 392
Psp5II RGGWCCY 2 cut(s) 55, 240
Psp6I CCWGG 2 cut(s) 42, 57
PspGI CCWGG 2 cut(s) 42, 57
PspN4I GGNNCC 2 cut(s) 241, 261
PspPI GGNCC 3 cut(s) 40, 55, 240
PspPPI RGGWCCY 2 cut(s) 55, 240
PsuI RGATCY 1 cut(s) 259
RruI TCGCGA 1 cut(s) 66
RsaI GTAC 1 cut(s) 291
RsaNI GTAC 1 cut(s) 290
RseI CAYNNNNRTG 1 cut(s) 382
SalI GTCGAC 1 cut(s) 8
SapI GCTCTTC 1 cut(s) 396
SaqAI TTAA 1 cut(s) 366
SatI GCNGC 4 cut(s) 15, 122, 462, 513
Sau3AI GATC 3 cut(s) 102, 157, 259
Sau96I GGNCC 3 cut(s) 40, 55, 240
ScrFI CCNGG 2 cut(s) 44, 59
SduI GDGCHC 1 cut(s) 538
SexAI ACCWGGT 1 cut(s) 57
SfaNI GCATC 1 cut(s) 119
SgrDI CGTCGACG 1 cut(s) 8
SinI GGWCC 3 cut(s) 40, 55, 240
SmiMI CAYNNNNRTG 1 cut(s) 382
Sse9I AATT 4 cut(s) 131, 181, 367, 496
SsiI CCGC 2 cut(s) 15, 281
SspMI CTAG 1 cut(s) 446
StyD4I CCNGG 2 cut(s) 42, 57
StyI CCWWGG 1 cut(s) 27
TaiI ACGT 1 cut(s) 377
TaqI TCGA 1 cut(s) 9
TasI AATT 4 cut(s) 131, 181, 367, 496
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 1 cut(s) 410
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TscAI CASTG 1 cut(s) 235
TseFI GTSAC 3 cut(s) 375, 417, 470
TseI GCWGC 3 cut(s) 121, 461, 512
Tsp45I GTSAC 3 cut(s) 375, 417, 470
TspRI CASTG 1 cut(s) 235
VpaK11BI GGWCC 3 cut(s) 40, 55, 240
XapI RAATTY 1 cut(s) 181
XceI RCATGY 1 cut(s) 145
XcmI CCANNNNNNNNNTGG 2 cut(s) 55, 181
XmiI GTMKAC 1 cut(s) 9
XspI CTAG 1 cut(s) 446
Zsp2I ATGCAT 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.