Rh3AG070800

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0001324
Physical Location & Seq
Forward (+)
5178524 .. 5178789
266 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG070800.1

Sequence Viewer

Length: 171 bp
ATGGGAGAGGAGAAAGCAGAGGCGTCGTTGGATTCGCTGATCGCCGGGTTCAATGCTCGGATCTCAGAGCTCCAAGAGCTCGTTATTGCGCGAAACATGTACCTAGCCAACAGCGTCACCGATTTGTCGCCTGTCAACGGCGCCGTAAAGGACATGGAGCTTCAGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

56

Amino Acids

6.05

Weight (kDa)

4.25

Isoelectric Point (pI)

46.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 9 - 56 1e-07 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 140
AccII CGCG 1 cut(s) 91
AclWI GGATC 1 cut(s) 68
AcuI CTGAAG 1 cut(s) 146
AcyI GRCGYC 2 cut(s) 23, 141
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 1 cut(s) 137
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 1 cut(s) 52
AluBI AGCT 3 cut(s) 70, 79, 160
AluI AGCT 3 cut(s) 70, 79, 160
Alw21I GWGCWC 2 cut(s) 72, 81
AlwI GGATC 1 cut(s) 68
AspLEI GCGC 2 cut(s) 91, 143
AsuC2I CCSGG 1 cut(s) 46
AsuHPI GGTGA 1 cut(s) 109
BanI GGYRCC 1 cut(s) 140
BanII GRGCYC 2 cut(s) 72, 81
Bbv12I GWGCWC 2 cut(s) 72, 81
BceAI ACGGC 2 cut(s) 128, 154
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BcnI CCSGG 1 cut(s) 46
BfaI CTAG 2 cut(s) 104, 169
BfoI RGCGCY 1 cut(s) 144
Bme1390I CCNGG 1 cut(s) 46
BmiI GGNNCC 1 cut(s) 142
BmrFI CCNGG 1 cut(s) 46
BpuMI CCSGG 1 cut(s) 46
BsaHI GRCGYC 2 cut(s) 23, 141
Bsc4I CCNNNNNNNGG 1 cut(s) 137
BseLI CCNNNNNNNGG 1 cut(s) 137
BseMII CTCAG 1 cut(s) 78
BseRI GAGGAG 1 cut(s) 23
Bsh1236I CGCG 1 cut(s) 91
BshNI GGYRCC 1 cut(s) 140
BsiHKAI GWGCWC 2 cut(s) 72, 81
BsiSI CCGG 1 cut(s) 45
BslI CCNNNNNNNGG 1 cut(s) 137
Bsp1286I GDGCHC 2 cut(s) 72, 81
Bsp143I GATC 2 cut(s) 39, 60
BspCNI CTCAG 1 cut(s) 77
BspFNI CGCG 1 cut(s) 91
BspLI GGNNCC 1 cut(s) 142
BspPI GGATC 1 cut(s) 68
BspT107I GGYRCC 1 cut(s) 140
BssMI GATC 2 cut(s) 39, 60
BssNI GRCGYC 2 cut(s) 23, 141
BstACI GRCGYC 2 cut(s) 23, 141
BstDEI CTNAG 1 cut(s) 64
BstFNI CGCG 1 cut(s) 91
BstH2I RGCGCY 1 cut(s) 144
BstHHI GCGC 2 cut(s) 91, 143
BstKTI GATC 2 cut(s) 42, 63
BstMBI GATC 2 cut(s) 39, 60
BstMWI GCNNNNNNNGC 1 cut(s) 76
BstNSI RCATGY 1 cut(s) 100
BstSCI CCNGG 1 cut(s) 44
BstUI CGCG 1 cut(s) 91
BstX2I RGATCY 1 cut(s) 60
BstYI RGATCY 1 cut(s) 60
CfoI GCGC 2 cut(s) 91, 143
CseI GACGC 2 cut(s) 12, 103
Csp6I GTAC 1 cut(s) 100
CviAII CATG 2 cut(s) 97, 154
CviJI RGCY 4 cut(s) 70, 79, 107, 160
CviKI_1 RGCY 4 cut(s) 70, 79, 107, 160
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 1 cut(s) 64
DinI GGCGCC 1 cut(s) 142
DpnI GATC 2 cut(s) 41, 62
DpnII GATC 2 cut(s) 39, 60
Ecl136II GAGCTC 2 cut(s) 70, 79
Eco24I GRGCYC 2 cut(s) 72, 81
Eco53kI GAGCTC 2 cut(s) 70, 79
Eco57I CTGAAG 1 cut(s) 146
EcoICRI GAGCTC 2 cut(s) 70, 79
EcoT38I GRGCYC 2 cut(s) 72, 81
EgeI GGCGCC 1 cut(s) 142
EheI GGCGCC 1 cut(s) 142
FaeI CATG 2 cut(s) 100, 157
FaiI YATR 2 cut(s) 98, 155
FatI CATG 2 cut(s) 96, 153
FriOI GRGCYC 2 cut(s) 72, 81
FspBI CTAG 2 cut(s) 104, 169
GlaI GCGC 2 cut(s) 90, 142
HaeII RGCGCY 1 cut(s) 144
HapII CCGG 1 cut(s) 45
HgaI GACGC 2 cut(s) 12, 103
HhaI GCGC 2 cut(s) 91, 143
Hin1I GRCGYC 2 cut(s) 23, 141
Hin1II CATG 2 cut(s) 100, 157
Hin6I GCGC 2 cut(s) 89, 141
HinP1I GCGC 2 cut(s) 89, 141
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HinfI GANTC 1 cut(s) 32
HpaII CCGG 1 cut(s) 45
HphI GGTGA 1 cut(s) 109
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 2 cut(s) 60, 67
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 1 cut(s) 28
HpyF10VI GCNNNNNNNGC 1 cut(s) 76
HpyF3I CTNAG 1 cut(s) 64
Hsp92I GRCGYC 2 cut(s) 23, 141
Hsp92II CATG 2 cut(s) 100, 157
HspAI GCGC 2 cut(s) 89, 141
KasI GGCGCC 1 cut(s) 140
Kzo9I GATC 2 cut(s) 39, 60
LmnI GCTCC 2 cut(s) 75, 157
LpnPI CCDG 3 cut(s) 58, 144, 149
MaeI CTAG 2 cut(s) 104, 169
MaeIII GTNAC 1 cut(s) 115
MalI GATC 2 cut(s) 41, 62
MboI GATC 2 cut(s) 39, 60
MflI RGATCY 1 cut(s) 60
MhlI GDGCHC 2 cut(s) 72, 81
Mly113I GGCGCC 1 cut(s) 141
MmeI TCCRAC 1 cut(s) 9
MnlI CCTC 1 cut(s) 13
MspI CCGG 1 cut(s) 45
MspR9I CCNGG 1 cut(s) 46
MvnI CGCG 1 cut(s) 91
MwoI GCNNNNNNNGC 1 cut(s) 76
NarI GGCGCC 1 cut(s) 141
NciI CCSGG 1 cut(s) 46
NdeII GATC 2 cut(s) 39, 60
NlaIII CATG 2 cut(s) 100, 157
NlaIV GGNNCC 1 cut(s) 142
NmuCI GTSAC 1 cut(s) 115
NspI RCATGY 1 cut(s) 100
PciI ACATGT 1 cut(s) 96
PfeI GAWTC 1 cut(s) 32
PluTI GGCGCC 1 cut(s) 144
PscI ACATGT 1 cut(s) 96
Psp124BI GAGCTC 2 cut(s) 72, 81
PspN4I GGNNCC 1 cut(s) 142
PsuI RGATCY 1 cut(s) 60
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SacI GAGCTC 2 cut(s) 72, 81
Sau3AI GATC 2 cut(s) 39, 60
ScrFI CCNGG 1 cut(s) 46
SduI GDGCHC 2 cut(s) 72, 81
SetI ASST 5 cut(s) 72, 81, 105, 162, 168
SfoI GGCGCC 1 cut(s) 142
SspDI GGCGCC 1 cut(s) 140
SspMI CTAG 2 cut(s) 104, 169
SstI GAGCTC 2 cut(s) 72, 81
StyD4I CCNGG 1 cut(s) 44
TfiI GAWTC 1 cut(s) 32
TseFI GTSAC 1 cut(s) 115
Tsp45I GTSAC 1 cut(s) 115
XceI RCATGY 1 cut(s) 100
XspI CTAG 2 cut(s) 104, 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.