Rh5BG202000

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001324
Physical Location & Seq
Reverse (-)
22806078 .. 22812729
6652 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG202000.1

Sequence Viewer

Length: 957 bp
ATGGAGGCGGAGAAAGCAGGGGCGTCGTTGGACTCGCTTATCGCCGGGTTCAACAGTCGGATCTCGGAGCTCAAAGAACTCGTTATTGCGCGAAACATGTACCCAGCCAGCAGCGTCACTGATTTGTCGGCCGTAGACGCCGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGCCATCAAGGACCGGGTTCGCGACGAAACCCTAGCCATCCCCAAAGCAAAAAAACTGATCGATGCATCGCTGAAGCAGCAAAGGAAATTGAAGGATATGTCAGTTTATGCCCCCGCTCATGTTCCTGAAAGGATGTCAATGTTGAATTTGGATACAAACAGATGTTTATTTCCAGAAAGCTCTCAGCAAAACACTGGTTCTGGGACCTTCAATCTTGACGAGGATCCTGCACCACCACCACCACCAAAGGAGAAAAAGGGCCGTTCCACACCACCATTGTGGTTTATAAGTACTGAAGAGTTGGATTCCTTGTCATCATACATGAGAGGAAGGCTTACACTAGAGAAGGTCAATGCAGCTATTAATGACATGGCAACATATGCTGAAGCAAATTATCAGCTCATAGCTGCTCCAAAGAAGAATGTGGCAGGGGATCGATGGGAAAAAGCCTTGGAAATAAGAGATATAGCAATGCATGAAGCAGTTAAGGGAAAACACTTCTTTCTCGAAACTGACATGAAGGGACCATCCTTGAAGCTTGACAACACTGGAAAAGCAATACTAACAGTCCTTCGTCACCTTGGTCGGGTAAATGAGACACGGATTGGTCATCATCGGGTTATTGCTACCCTTGCATCTGGGGAGACAAGTTATGGAGAATGTCCCATCTTGGCAACCCATGATGCGATTGAATTTGCTTCTTGTGATAGACCAAGTAAAGTTGTGCAAGGGCGTGCATCTTTTAAGCTCAAGATATCTCAGATACTGAGATACAGAAAAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

318

Amino Acids

35.2

Weight (kDa)

9.06

Isoelectric Point (pI)

42.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 9 - 261 1e-85 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 458
AccBSI CCGCTC 1 cut(s) 287
AccI GTMKAC 1 cut(s) 135
AccII CGCG 2 cut(s) 91, 192
AciI CCGC 3 cut(s) 8, 141, 285
AclWI GGATC 4 cut(s) 68, 389, 402, 612
AcoI YGGCCR 1 cut(s) 129
AcsI RAATTY 2 cut(s) 316, 863
AcuI CTGAAG 4 cut(s) 146, 263, 486, 576
AcyI GRCGYC 2 cut(s) 23, 138
AfaI GTAC 2 cut(s) 101, 463
AfiI CCNNNNNNNGG 1 cut(s) 757
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 6 cut(s) 52, 262, 316, 382, 706, 863
AjnI CCWGG 1 cut(s) 168
AleI CACNNNNGTG 1 cut(s) 448
AluBI AGCT 8 cut(s) 70, 160, 351, 530, 571, 578, 709, 919
AluI AGCT 8 cut(s) 70, 160, 351, 530, 571, 578, 709, 919
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 2 cut(s) 761, 809
AlwI GGATC 4 cut(s) 68, 389, 402, 612
AlwNI CAGNNNCTG 1 cut(s) 937
AoxI GGCC 4 cut(s) 129, 150, 171, 430
ApeKI GCWGC 4 cut(s) 111, 247, 527, 578
ApoI RAATTY 2 cut(s) 316, 863
AseI ATTAAT 1 cut(s) 534
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 5 cut(s) 166, 181, 375, 430, 695
AsuC2I CCSGG 2 cut(s) 46, 185
AsuHPI GGTGA 1 cut(s) 740
AvaII GGWCC 4 cut(s) 166, 181, 375, 695
BamHI GGATCC 1 cut(s) 394
BanII GRGCYC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 72
BbvI GCAGC 4 cut(s) 123, 259, 539, 565
BccI CCATC 5 cut(s) 182, 215, 603, 706, 845
BceAI ACGGC 3 cut(s) 116, 128, 417
BcgI CGANNNNNNTGC 4 cut(s) 6, 40, 380, 414
BciT130I CCWGG 1 cut(s) 170
BciVI GTATCC 1 cut(s) 316
BcnI CCSGG 2 cut(s) 46, 185
BcoDI GTCTC 2 cut(s) 761, 809
BfaI CTAG 2 cut(s) 203, 512
BfuI GTATCC 1 cut(s) 316
BglI GCCNNNNNGGC 1 cut(s) 149
BisI GCNGC 5 cut(s) 112, 141, 248, 528, 579
BlsI GCNGC 5 cut(s) 113, 142, 249, 529, 580
BmcAI AGTACT 1 cut(s) 463
Bme1390I CCNGG 3 cut(s) 46, 170, 185
Bme18I GGWCC 4 cut(s) 166, 181, 375, 695
BmgT120I GGNCC 5 cut(s) 166, 181, 375, 430, 695
BmiI GGNNCC 3 cut(s) 376, 396, 696
BmrFI CCNGG 3 cut(s) 46, 170, 185
BmsI GCATC 5 cut(s) 223, 245, 815, 844, 917
BpuEI CTTGAG 1 cut(s) 905
BpuMI CCSGG 2 cut(s) 46, 185
Bsa29I ATCGAT 2 cut(s) 231, 607
BsaHI GRCGYC 2 cut(s) 23, 138
BsaJI CCNNGG 3 cut(s) 153, 621, 751
Bsc4I CCNNNNNNNGG 1 cut(s) 757
Bse1I ACTGG 2 cut(s) 370, 724
Bse3DI GCAATG 1 cut(s) 648
BseBI CCWGG 1 cut(s) 170
BseCI ATCGAT 2 cut(s) 231, 607
BseDI CCNNGG 3 cut(s) 153, 621, 751
BseGI GGATG 3 cut(s) 207, 309, 698
BseLI CCNNNNNNNGG 1 cut(s) 757
BseMI GCAATG 1 cut(s) 648
BseMII CTCAG 3 cut(s) 368, 929, 944
BseNI ACTGG 2 cut(s) 370, 724
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 4 cut(s) 123, 259, 539, 565
BseYI CCCAGC 1 cut(s) 103
BsgI GTGCAG 1 cut(s) 384
Bsh1236I CGCG 2 cut(s) 91, 192
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 4 cut(s) 131, 152, 173, 432
BshVI ATCGAT 2 cut(s) 231, 607
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 72
BsiSI CCGG 2 cut(s) 45, 184
BslFI GGGAC 3 cut(s) 388, 708, 819
BslI CCNNNNNNNGG 1 cut(s) 757
BsmAI GTCTC 2 cut(s) 761, 809
BsmFI GGGAC 3 cut(s) 388, 708, 819
BsnI GGCC 4 cut(s) 131, 152, 173, 432
Bsp1286I GDGCHC 1 cut(s) 72
Bsp143I GATC 4 cut(s) 60, 228, 394, 604
Bsp19I CCATGG 1 cut(s) 153
Bsp68I TCGCGA 1 cut(s) 192
BspACI CCGC 3 cut(s) 8, 141, 285
BspANI GGCC 4 cut(s) 131, 152, 173, 432
BspCNI CTCAG 3 cut(s) 367, 930, 943
BspDI ATCGAT 2 cut(s) 231, 607
BspFNI CGCG 2 cut(s) 91, 192
BspLI GGNNCC 3 cut(s) 376, 396, 696
BspPI GGATC 4 cut(s) 68, 389, 402, 612
BsrBI CCGCTC 1 cut(s) 287
BsrDI GCAATG 1 cut(s) 648
BsrI ACTGG 2 cut(s) 370, 724
BssECI CCNNGG 3 cut(s) 153, 621, 751
BssMI GATC 4 cut(s) 60, 228, 394, 604
BssNI GRCGYC 2 cut(s) 23, 138
BssT1I CCWWGG 3 cut(s) 153, 621, 751
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 2 cut(s) 56, 739
Bst6I CTCTTC 1 cut(s) 462
BstACI GRCGYC 2 cut(s) 23, 138
BstAPI GCANNNNNTGC 1 cut(s) 551
BstC8I GCNNGC 2 cut(s) 109, 906
BstDEI CTNAG 3 cut(s) 354, 930, 938
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 3 cut(s) 207, 309, 698
BstFNI CGCG 2 cut(s) 91, 192
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 4 cut(s) 63, 231, 397, 607
BstMAI GTCTC 2 cut(s) 761, 809
BstMBI GATC 4 cut(s) 60, 228, 394, 604
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 6 cut(s) 14, 137, 149, 247, 551, 803
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 100
BstSCI CCNGG 3 cut(s) 44, 168, 183
BstUI CGCG 2 cut(s) 91, 192
BstV1I GCAGC 4 cut(s) 123, 259, 539, 565
BstX2I RGATCY 2 cut(s) 60, 394
BstXI CCANNNNNNTGG 1 cut(s) 450
BstYI RGATCY 2 cut(s) 60, 394
BstZI CGGCCG 1 cut(s) 129
Bsu15I ATCGAT 2 cut(s) 231, 607
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 4 cut(s) 131, 152, 173, 432
BsuTUI ATCGAT 2 cut(s) 231, 607
BtgI CCRYGG 1 cut(s) 153
BtgZI GCGATG 1 cut(s) 222
BtsCI GGATG 3 cut(s) 207, 309, 698
BtsIMutI CAGTG 3 cut(s) 117, 363, 717
BtuMI TCGCGA 1 cut(s) 192
Cac8I GCNNGC 2 cut(s) 109, 906
CaiI CAGNNNCTG 1 cut(s) 937
CfoI GCGC 1 cut(s) 91
Cfr13I GGNCC 5 cut(s) 166, 181, 375, 430, 695
ClaI ATCGAT 2 cut(s) 231, 607
CseI GACGC 3 cut(s) 12, 103, 146
Csp6I GTAC 2 cut(s) 100, 462
CviAII CATG 8 cut(s) 97, 154, 290, 493, 541, 647, 688, 851
CviQI GTAC 2 cut(s) 100, 462
DdeI CTNAG 3 cut(s) 354, 930, 938
DpnI GATC 4 cut(s) 62, 230, 396, 606
DpnII GATC 4 cut(s) 60, 228, 394, 604
EaeI YGGCCR 1 cut(s) 129
EagI CGGCCG 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 462
EarI CTCTTC 1 cut(s) 462
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 1 cut(s) 70
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 3 cut(s) 153, 621, 751
Eco24I GRGCYC 1 cut(s) 72
Eco32I GATATC 1 cut(s) 927
Eco47I GGWCC 4 cut(s) 166, 181, 375, 695
Eco52I CGGCCG 1 cut(s) 129
Eco53kI GAGCTC 1 cut(s) 70
Eco57I CTGAAG 4 cut(s) 146, 263, 486, 576
EcoICRI GAGCTC 1 cut(s) 70
EcoO109I RGGNCCY 1 cut(s) 375
EcoRII CCWGG 1 cut(s) 168
EcoRV GATATC 1 cut(s) 927
EcoT14I CCWWGG 3 cut(s) 153, 621, 751
EcoT22I ATGCAT 2 cut(s) 238, 648
EcoT38I GRGCYC 1 cut(s) 72
ErhI CCWWGG 3 cut(s) 153, 621, 751
FaeI CATG 8 cut(s) 100, 157, 293, 496, 544, 650, 691, 854
FaqI GGGAC 3 cut(s) 388, 708, 819
FatI CATG 8 cut(s) 96, 153, 289, 492, 540, 646, 687, 850
FauI CCCGC 1 cut(s) 292
FauNDI CATATG 1 cut(s) 550
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 5 cut(s) 112, 141, 248, 528, 579
FokI GGATG 3 cut(s) 194, 316, 685
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 5 cut(s) 112, 141, 248, 528, 579
FspBI CTAG 2 cut(s) 203, 512
GlaI GCGC 1 cut(s) 90
GluI GCNGC 5 cut(s) 112, 141, 248, 528, 579
GsaI CCCAGC 1 cut(s) 107
HaeIII GGCC 4 cut(s) 131, 152, 173, 432
HapII CCGG 2 cut(s) 45, 184
HgaI GACGC 3 cut(s) 12, 103, 146
HhaI GCGC 1 cut(s) 91
Hin1I GRCGYC 2 cut(s) 23, 138
Hin1II CATG 8 cut(s) 100, 157, 293, 496, 544, 650, 691, 854
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HindIII AAGCTT 1 cut(s) 707
HinfI GANTC 2 cut(s) 32, 476
HpaII CCGG 2 cut(s) 45, 184
HphI GGTGA 1 cut(s) 740
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 3 cut(s) 60, 67, 933
Hpy188III TCNNGA 6 cut(s) 191, 296, 344, 386, 677, 922
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 2 cut(s) 28, 197
HpyAV CCTTC 7 cut(s) 142, 256, 388, 495, 511, 685, 752
HpyCH4III ACNGT 2 cut(s) 56, 739
HpyCH4V TGCA 7 cut(s) 236, 401, 527, 646, 806, 898, 908
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 137, 149, 247, 551, 803
HpyF3I CTNAG 3 cut(s) 354, 930, 938
Hsp92I GRCGYC 2 cut(s) 23, 138
Hsp92II CATG 8 cut(s) 100, 157, 293, 496, 544, 650, 691, 854
HspAI GCGC 1 cut(s) 89
Kzo9I GATC 4 cut(s) 60, 228, 394, 604
LmnI GCTCC 3 cut(s) 67, 157, 586
Lsp1109I GCAGC 4 cut(s) 123, 259, 539, 565
LweI GCATC 5 cut(s) 223, 245, 815, 844, 917
MaeI CTAG 2 cut(s) 203, 512
MaeIII GTNAC 2 cut(s) 115, 746
MalI GATC 4 cut(s) 62, 230, 396, 606
MbiI CCGCTC 1 cut(s) 287
MboI GATC 4 cut(s) 60, 228, 394, 604
MboII GAAGA 2 cut(s) 479, 601
MflI RGATCY 2 cut(s) 60, 394
MhlI GDGCHC 1 cut(s) 72
MluCI AATT 4 cut(s) 257, 316, 562, 863
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 3 cut(s) 9, 38, 453
MnlI CCTC 2 cut(s) 385, 491
Mph1103I ATGCAT 2 cut(s) 238, 648
MseI TTAA 3 cut(s) 534, 657, 915
MslI CAYNNNNRTG 1 cut(s) 448
MspI CCGG 2 cut(s) 45, 184
MspR9I CCNGG 3 cut(s) 46, 170, 185
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 2 cut(s) 91, 192
MwoI GCNNNNNNNGC 6 cut(s) 14, 137, 149, 247, 551, 803
NciI CCSGG 2 cut(s) 46, 185
NcoI CCATGG 1 cut(s) 153
NdeI CATATG 1 cut(s) 550
NdeII GATC 4 cut(s) 60, 228, 394, 604
NlaIII CATG 8 cut(s) 100, 157, 293, 496, 544, 650, 691, 854
NlaIV GGNNCC 3 cut(s) 376, 396, 696
NmuCI GTSAC 2 cut(s) 115, 746
NruI TCGCGA 1 cut(s) 192
NsiI ATGCAT 2 cut(s) 238, 648
NspI RCATGY 1 cut(s) 100
OliI CACNNNNGTG 1 cut(s) 448
PciI ACATGT 1 cut(s) 96
PfeI GAWTC 1 cut(s) 476
PkrI GCNGC 5 cut(s) 113, 142, 249, 529, 580
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PpuMI RGGWCCY 1 cut(s) 375
PscI ACATGT 1 cut(s) 96
PshBI ATTAAT 1 cut(s) 534
PsiI TTATAA 1 cut(s) 458
Psp124BI GAGCTC 1 cut(s) 72
Psp5II RGGWCCY 1 cut(s) 375
Psp6I CCWGG 1 cut(s) 168
PspFI CCCAGC 1 cut(s) 103
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 3 cut(s) 376, 396, 696
PspPI GGNCC 5 cut(s) 166, 181, 375, 430, 695
PspPPI RGGWCCY 1 cut(s) 375
PstNI CAGNNNCTG 1 cut(s) 937
PsuI RGATCY 2 cut(s) 60, 394
RruI TCGCGA 1 cut(s) 192
RsaI GTAC 2 cut(s) 101, 463
RsaNI GTAC 2 cut(s) 100, 462
RseI CAYNNNNRTG 1 cut(s) 448
SacI GAGCTC 1 cut(s) 72
SaqAI TTAA 3 cut(s) 534, 657, 915
SatI GCNGC 5 cut(s) 112, 141, 248, 528, 579
Sau3AI GATC 4 cut(s) 60, 228, 394, 604
Sau96I GGNCC 5 cut(s) 166, 181, 375, 430, 695
ScaI AGTACT 1 cut(s) 463
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 3 cut(s) 46, 170, 185
SduI GDGCHC 1 cut(s) 72
SfaNI GCATC 5 cut(s) 223, 245, 815, 844, 917
SinI GGWCC 4 cut(s) 166, 181, 375, 695
SmiMI CAYNNNNRTG 1 cut(s) 448
SmlI CTYRAG 1 cut(s) 920
SmoI CTYRAG 1 cut(s) 920
Sse9I AATT 4 cut(s) 257, 316, 562, 863
SsiI CCGC 3 cut(s) 8, 141, 285
SspMI CTAG 2 cut(s) 203, 512
SstI GAGCTC 1 cut(s) 72
StyD4I CCNGG 3 cut(s) 44, 168, 183
StyI CCWWGG 3 cut(s) 153, 621, 751
TaaI ACNGT 2 cut(s) 56, 739
TaqI TCGA 3 cut(s) 231, 607, 678
TasI AATT 4 cut(s) 257, 316, 562, 863
TatI WGTACW 1 cut(s) 461
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 1 cut(s) 476
Tru1I TTAA 3 cut(s) 534, 657, 915
Tru9I TTAA 3 cut(s) 534, 657, 915
TscAI CASTG 3 cut(s) 124, 370, 724
TseFI GTSAC 2 cut(s) 115, 746
TseI GCWGC 4 cut(s) 111, 247, 527, 578
Tsp45I GTSAC 2 cut(s) 115, 746
TspDTI ATGAA 2 cut(s) 663, 704
TspGWI ACGGA 1 cut(s) 787
TspRI CASTG 3 cut(s) 124, 370, 724
VpaK11BI GGWCC 4 cut(s) 166, 181, 375, 695
VspI ATTAAT 1 cut(s) 534
XapI RAATTY 2 cut(s) 316, 863
XceI RCATGY 1 cut(s) 100
XmiI GTMKAC 1 cut(s) 135
XspI CTAG 2 cut(s) 203, 512
ZrmI AGTACT 1 cut(s) 463
Zsp2I ATGCAT 2 cut(s) 238, 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.