Rh1CG203200

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0001324
Physical Location & Seq
Forward (+)
43988602 .. 44074273
85672 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG203200.1

Sequence Viewer

Length: 822 bp
ATGGAGGCGGAGAAAGCAGGGGCATCGCTGGACTCGCTGATCGCCGGGTTCAACAGTCGGATCTCGGAGCTCCAAGAGCTCGTTATTGCGCGAAACATGTACCCGGCCAGCACAGTCTCCGATTTGTCGGCCGTCGATGCCGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGCCATCAAGGACCGGGTTCGCGACGAAACCCTTGCCATCCCCAAAGCAAAAAAACTGATCGATGCATCGCTGAAGCAGCAAAGGAAATTGAGGGATATGTCAGTTTATGCCCCACCTCATGTTCCAGAAAGGATGTCAATGTTGAATCTGGATACAAACAGATGTTTACTTCCAGAAAGCTCTCAGCAAAACAATGGTTCTGGGACCTTCAAGCTTGACAAGGATCCTGCACCACCACCACCACCACCTAAGGAGAAAAATGGTCGTTCCACCCCACCATTGTGGTTTATTAGTGCCGAAGAGCTGGATTCCTTGTCATCATACATGAGAGGAAGGTTTACACTAGAGAAGGTCAATGCAGCTATTAGTGACATGGCAACATATGCTGAAGCAAATTATCAGCTCATAGCTGCTCCAAAGAAGAATGTGGCAGGAGATCGATGGGAAAAAGCCTTGGAAATAAGAGATATAGCAATGCATAAAGCAGTAAAGGGAAAACACTTCTTTCTCGAAACTGACATGAAGGGACCATCCTTGAAGCTTGACAACACTGGAAAAGCAATACTAACTGTCCTTCGTCACCTTGGTCGACGTGTAAGTGAGACACGGATTGGTCATCATCGGGTGATCATTCTTTTGAAGCCTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

273

Amino Acids

30.34

Weight (kDa)

9.46

Isoelectric Point (pI)

46.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 9 - 263 1.4e-81 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 760
AccII CGCG 2 cut(s) 91, 192
AciI CCGC 2 cut(s) 8, 141
AclWI GGATC 3 cut(s) 68, 389, 402
AcoI YGGCCR 2 cut(s) 105, 129
AcuI CTGAAG 3 cut(s) 146, 263, 579
AfaI GTAC 1 cut(s) 101
AflIII ACRYGT 2 cut(s) 96, 763
AgsI TTSAA 5 cut(s) 52, 316, 382, 709, 811
AjiI CACGTC 1 cut(s) 764
AjnI CCWGG 1 cut(s) 168
AleI CACNNNNGTG 1 cut(s) 451
Alw21I GWGCWC 2 cut(s) 72, 81
Alw26I GTCTC 2 cut(s) 121, 767
AlwI GGATC 3 cut(s) 68, 389, 402
AoxI GGCC 4 cut(s) 105, 129, 150, 171
ApeKI GCWGC 3 cut(s) 247, 530, 581
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 4 cut(s) 166, 181, 375, 698
AsuC2I CCSGG 3 cut(s) 46, 104, 185
AsuHPI GGTGA 2 cut(s) 743, 808
AvaII GGWCC 4 cut(s) 166, 181, 375, 698
AxyI CCTNAGG 1 cut(s) 420
BamHI GGATCC 1 cut(s) 394
BanII GRGCYC 2 cut(s) 72, 81
Bbv12I GWGCWC 2 cut(s) 72, 81
BbvI GCAGC 3 cut(s) 259, 542, 568
BccI CCATC 4 cut(s) 182, 215, 606, 709
BceAI ACGGC 2 cut(s) 116, 128
BcgI CGANNNNNNTGC 4 cut(s) 6, 40, 185, 219
BciT130I CCWGG 1 cut(s) 170
BciVI GTATCC 1 cut(s) 316
BclI TGATCA 1 cut(s) 798
BcnI CCSGG 3 cut(s) 46, 104, 185
BcoDI GTCTC 2 cut(s) 121, 767
BfaI CTAG 1 cut(s) 515
BfuI GTATCC 1 cut(s) 316
BglI GCCNNNNNGGC 1 cut(s) 149
BisI GCNGC 4 cut(s) 141, 248, 531, 582
BlsI GCNGC 4 cut(s) 142, 249, 532, 583
Bme1390I CCNGG 4 cut(s) 46, 104, 170, 185
Bme18I GGWCC 4 cut(s) 166, 181, 375, 698
BmgBI CACGTC 1 cut(s) 764
BmgT120I GGNCC 4 cut(s) 166, 181, 375, 698
BmiI GGNNCC 3 cut(s) 376, 396, 699
BmrFI CCNGG 4 cut(s) 46, 104, 170, 185
BmsI GCATC 4 cut(s) 32, 127, 223, 245
BpuMI CCSGG 3 cut(s) 46, 104, 185
Bsa29I ATCGAT 2 cut(s) 231, 610
BsaJI CCNNGG 3 cut(s) 153, 624, 754
Bse1I ACTGG 1 cut(s) 727
Bse21I CCTNAGG 1 cut(s) 420
Bse3DI GCAATG 1 cut(s) 651
BseBI CCWGG 1 cut(s) 170
BseCI ATCGAT 2 cut(s) 231, 610
BseDI CCNNGG 3 cut(s) 153, 624, 754
BseGI GGATG 3 cut(s) 207, 309, 701
BseMI GCAATG 1 cut(s) 651
BseMII CTCAG 1 cut(s) 368
BseNI ACTGG 1 cut(s) 727
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 3 cut(s) 259, 542, 568
BsgI GTGCAG 1 cut(s) 384
Bsh1236I CGCG 2 cut(s) 91, 192
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 4 cut(s) 107, 131, 152, 173
BshVI ATCGAT 2 cut(s) 231, 610
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 2 cut(s) 72, 81
BsiSI CCGG 3 cut(s) 45, 104, 184
BslFI GGGAC 2 cut(s) 388, 711
BsmAI GTCTC 2 cut(s) 121, 767
BsmFI GGGAC 2 cut(s) 388, 711
BsnI GGCC 4 cut(s) 107, 131, 152, 173
Bsp1286I GDGCHC 2 cut(s) 72, 81
Bsp143I GATC 6 cut(s) 39, 60, 228, 394, 607, 798
Bsp19I CCATGG 1 cut(s) 153
Bsp68I TCGCGA 1 cut(s) 192
BspACI CCGC 2 cut(s) 8, 141
BspANI GGCC 4 cut(s) 107, 131, 152, 173
BspCNI CTCAG 1 cut(s) 367
BspDI ATCGAT 2 cut(s) 231, 610
BspFNI CGCG 2 cut(s) 91, 192
BspLI GGNNCC 3 cut(s) 376, 396, 699
BspPI GGATC 3 cut(s) 68, 389, 402
BspQI GCTCTTC 1 cut(s) 465
BsrDI GCAATG 1 cut(s) 651
BsrI ACTGG 1 cut(s) 727
BssECI CCNNGG 3 cut(s) 153, 624, 754
BssMI GATC 6 cut(s) 39, 60, 228, 394, 607, 798
BssT1I CCWWGG 3 cut(s) 153, 624, 754
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 3 cut(s) 56, 115, 742
Bst6I CTCTTC 1 cut(s) 465
BstAPI GCANNNNNTGC 1 cut(s) 554
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 2 cut(s) 354, 420
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 3 cut(s) 207, 309, 701
BstFNI CGCG 2 cut(s) 91, 192
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 6 cut(s) 42, 63, 231, 397, 610, 801
BstMAI GTCTC 2 cut(s) 121, 767
BstMBI GATC 6 cut(s) 39, 60, 228, 394, 607, 798
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 7 cut(s) 14, 34, 76, 137, 149, 247, 554
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 100
BstSCI CCNGG 4 cut(s) 44, 102, 168, 183
BstUI CGCG 2 cut(s) 91, 192
BstV1I GCAGC 3 cut(s) 259, 542, 568
BstX2I RGATCY 2 cut(s) 60, 394
BstXI CCANNNNNNTGG 1 cut(s) 453
BstYI RGATCY 2 cut(s) 60, 394
BstZI CGGCCG 1 cut(s) 129
Bsu15I ATCGAT 2 cut(s) 231, 610
Bsu36I CCTNAGG 1 cut(s) 420
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 4 cut(s) 107, 131, 152, 173
BsuTUI ATCGAT 2 cut(s) 231, 610
BtgI CCRYGG 1 cut(s) 153
BtgZI GCGATG 2 cut(s) 9, 222
BtrI CACGTC 1 cut(s) 764
BtsCI GGATG 3 cut(s) 207, 309, 701
BtsIMutI CAGTG 1 cut(s) 720
BtuMI TCGCGA 1 cut(s) 192
Cac8I GCNNGC 1 cut(s) 109
CfoI GCGC 1 cut(s) 91
Cfr13I GGNCC 4 cut(s) 166, 181, 375, 698
ClaI ATCGAT 2 cut(s) 231, 610
Csp6I GTAC 1 cut(s) 100
CviAII CATG 6 cut(s) 97, 154, 290, 496, 544, 691
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 2 cut(s) 354, 420
DpnI GATC 6 cut(s) 41, 62, 230, 396, 609, 800
DpnII GATC 6 cut(s) 39, 60, 228, 394, 607, 798
EaeI YGGCCR 2 cut(s) 105, 129
EagI CGGCCG 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 465
EarI CTCTTC 1 cut(s) 465
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 2 cut(s) 70, 79
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 3 cut(s) 153, 624, 754
Eco24I GRGCYC 2 cut(s) 72, 81
Eco47I GGWCC 4 cut(s) 166, 181, 375, 698
Eco52I CGGCCG 1 cut(s) 129
Eco53kI GAGCTC 2 cut(s) 70, 79
Eco57I CTGAAG 3 cut(s) 146, 263, 579
Eco81I CCTNAGG 1 cut(s) 420
EcoICRI GAGCTC 2 cut(s) 70, 79
EcoO109I RGGNCCY 1 cut(s) 375
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 3 cut(s) 153, 624, 754
EcoT22I ATGCAT 2 cut(s) 238, 651
EcoT38I GRGCYC 2 cut(s) 72, 81
ErhI CCWWGG 3 cut(s) 153, 624, 754
FaeI CATG 6 cut(s) 100, 157, 293, 499, 547, 694
FaqI GGGAC 2 cut(s) 388, 711
FatI CATG 6 cut(s) 96, 153, 289, 495, 543, 690
FauNDI CATATG 1 cut(s) 553
FbaI TGATCA 1 cut(s) 798
FblI GTMKAC 1 cut(s) 760
Fnu4HI GCNGC 4 cut(s) 141, 248, 531, 582
FokI GGATG 3 cut(s) 194, 316, 688
FriOI GRGCYC 2 cut(s) 72, 81
Fsp4HI GCNGC 4 cut(s) 141, 248, 531, 582
FspBI CTAG 1 cut(s) 515
GlaI GCGC 1 cut(s) 90
GluI GCNGC 4 cut(s) 141, 248, 531, 582
HaeIII GGCC 4 cut(s) 107, 131, 152, 173
HapII CCGG 3 cut(s) 45, 104, 184
HhaI GCGC 1 cut(s) 91
Hin1II CATG 6 cut(s) 100, 157, 293, 499, 547, 694
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HincII GTYRAC 1 cut(s) 761
HindII GTYRAC 1 cut(s) 761
HindIII AAGCTT 2 cut(s) 383, 710
HinfI GANTC 3 cut(s) 32, 316, 479
HpaII CCGG 3 cut(s) 45, 104, 184
HphI GGTGA 2 cut(s) 743, 808
Hpy166II GTNNAC 3 cut(s) 338, 510, 761
Hpy188I TCNGA 3 cut(s) 60, 67, 121
Hpy188III TCNNGA 5 cut(s) 191, 296, 320, 344, 680
Hpy8I GTNNAC 3 cut(s) 338, 510, 761
Hpy99I CGWCG 3 cut(s) 137, 197, 765
HpyAV CCTTC 6 cut(s) 142, 388, 498, 514, 688, 755
HpyCH4III ACNGT 3 cut(s) 56, 115, 742
HpyCH4IV ACGT 1 cut(s) 763
HpyCH4V TGCA 4 cut(s) 236, 401, 530, 649
HpyF10VI GCNNNNNNNGC 7 cut(s) 14, 34, 76, 137, 149, 247, 554
HpyF3I CTNAG 2 cut(s) 354, 420
HpySE526I ACGT 1 cut(s) 763
Hsp92II CATG 6 cut(s) 100, 157, 293, 499, 547, 694
HspAI GCGC 1 cut(s) 89
Ksp22I TGATCA 1 cut(s) 798
Kzo9I GATC 6 cut(s) 39, 60, 228, 394, 607, 798
LguI GCTCTTC 1 cut(s) 465
LmnI GCTCC 4 cut(s) 67, 75, 157, 589
Lsp1109I GCAGC 3 cut(s) 259, 542, 568
LweI GCATC 4 cut(s) 32, 127, 223, 245
MaeI CTAG 1 cut(s) 515
MaeII ACGT 1 cut(s) 763
MaeIII GTNAC 2 cut(s) 539, 749
MalI GATC 6 cut(s) 41, 62, 230, 396, 609, 800
MboI GATC 6 cut(s) 39, 60, 228, 394, 607, 798
MboII GAAGA 2 cut(s) 482, 604
MflI RGATCY 2 cut(s) 60, 394
MhlI GDGCHC 2 cut(s) 72, 81
MluCI AATT 2 cut(s) 257, 565
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 1 cut(s) 38
MnlI CCTC 3 cut(s) 255, 297, 494
Mph1103I ATGCAT 2 cut(s) 238, 651
MslI CAYNNNNRTG 1 cut(s) 451
MspI CCGG 3 cut(s) 45, 104, 184
MspR9I CCNGG 4 cut(s) 46, 104, 170, 185
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 2 cut(s) 91, 192
MwoI GCNNNNNNNGC 7 cut(s) 14, 34, 76, 137, 149, 247, 554
NciI CCSGG 3 cut(s) 46, 104, 185
NcoI CCATGG 1 cut(s) 153
NdeI CATATG 1 cut(s) 553
NdeII GATC 6 cut(s) 39, 60, 228, 394, 607, 798
NlaIII CATG 6 cut(s) 100, 157, 293, 499, 547, 694
NlaIV GGNNCC 3 cut(s) 376, 396, 699
NmuCI GTSAC 2 cut(s) 539, 749
NruI TCGCGA 1 cut(s) 192
NsiI ATGCAT 2 cut(s) 238, 651
NspI RCATGY 1 cut(s) 100
OliI CACNNNNGTG 1 cut(s) 451
PciI ACATGT 1 cut(s) 96
PciSI GCTCTTC 1 cut(s) 465
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PfeI GAWTC 2 cut(s) 316, 479
PkrI GCNGC 4 cut(s) 142, 249, 532, 583
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PpuMI RGGWCCY 1 cut(s) 375
PscI ACATGT 1 cut(s) 96
Psp124BI GAGCTC 2 cut(s) 72, 81
Psp5II RGGWCCY 1 cut(s) 375
Psp6I CCWGG 1 cut(s) 168
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 3 cut(s) 376, 396, 699
PspPI GGNCC 4 cut(s) 166, 181, 375, 698
PspPPI RGGWCCY 1 cut(s) 375
PsuI RGATCY 2 cut(s) 60, 394
RruI TCGCGA 1 cut(s) 192
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
RseI CAYNNNNRTG 1 cut(s) 451
SacI GAGCTC 2 cut(s) 72, 81
SalI GTCGAC 1 cut(s) 759
SapI GCTCTTC 1 cut(s) 465
SatI GCNGC 4 cut(s) 141, 248, 531, 582
Sau3AI GATC 6 cut(s) 39, 60, 228, 394, 607, 798
Sau96I GGNCC 4 cut(s) 166, 181, 375, 698
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 4 cut(s) 46, 104, 170, 185
SduI GDGCHC 2 cut(s) 72, 81
SfaNI GCATC 4 cut(s) 32, 127, 223, 245
SinI GGWCC 4 cut(s) 166, 181, 375, 698
SmiMI CAYNNNNRTG 1 cut(s) 451
Sse9I AATT 2 cut(s) 257, 565
SsiI CCGC 2 cut(s) 8, 141
SspMI CTAG 1 cut(s) 515
SstI GAGCTC 2 cut(s) 72, 81
StyD4I CCNGG 4 cut(s) 44, 102, 168, 183
StyI CCWWGG 3 cut(s) 153, 624, 754
TaaI ACNGT 3 cut(s) 56, 115, 742
TaiI ACGT 1 cut(s) 766
TaqI TCGA 5 cut(s) 135, 231, 610, 681, 760
TasI AATT 2 cut(s) 257, 565
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 2 cut(s) 316, 479
TscAI CASTG 1 cut(s) 727
TseFI GTSAC 2 cut(s) 539, 749
TseI GCWGC 3 cut(s) 247, 530, 581
Tsp45I GTSAC 2 cut(s) 539, 749
TspDTI ATGAA 1 cut(s) 707
TspGWI ACGGA 1 cut(s) 793
TspRI CASTG 1 cut(s) 727
VpaK11BI GGWCC 4 cut(s) 166, 181, 375, 698
XceI RCATGY 1 cut(s) 100
XmiI GTMKAC 1 cut(s) 760
XspI CTAG 1 cut(s) 515
Zsp2I ATGCAT 2 cut(s) 238, 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.