RLG00000028575

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001324
Physical Location & Seq
Reverse (-)
24533102 .. 24535206
2105 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028575

Sequence Viewer

Length: 573 bp
ATGGAGGCTGAGAAAGCAGGAGCGTCGTTGGACTCGCTGATCACTGGGTTCAACAGTCGGATCTCGGAGCTCCAAGAACTCGTTATTGCTCGAAACAGTCAGACCCGACCCGATTACCCAGCCCTTTTTCAATTCAATTTCGTCTCCAATAACAATACCGTCACCGATTTGACGGCCGTCGACGCCGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGCCATCAAGGACCTGGTTCGCGACGAAACCCTCGCCATCCCCAAAGCAAAATTGAATTTGGATACAAATAGATATTTATTTCCAGATAGCTCTCAGCAAAACACTGGTTCTGGGACCTTCAAGCTTGATGAGGATCCTGCACCACCACCAGCACCACCTAAGGAGAAAAAGGGTCGTTCCACGTCACCATTGTGGTTTATAAGTGCTGAAGAGCTGGATTCCTTGTCACCATACATGAGAGGAAGGCTTACACTAGAGAAGGTCAATGCAGCTATTAGTGACATGGCAACATATGCTGAAGCAAATTATCAGCCCGTAGCTGCTCCAAAGAAGAATGTTTGTGCTCTGCCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

191

Amino Acids

20.79

Weight (kDa)

4.98

Isoelectric Point (pI)

42.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 9 - 184 1.6e-29 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 416
AccI GTMKAC 1 cut(s) 180
AccII CGCG 1 cut(s) 237
AciI CCGC 1 cut(s) 186
AclWI GGATC 3 cut(s) 68, 344, 357
AcoI YGGCCR 1 cut(s) 174
AcsI RAATTY 1 cut(s) 271
AcuI CTGAAG 3 cut(s) 191, 444, 534
AcyI GRCGYC 1 cut(s) 183
AgsI TTSAA 5 cut(s) 52, 131, 136, 271, 337
AjiI CACGTC 1 cut(s) 399
AjnI CCWGG 2 cut(s) 213, 228
AleI CACNNNNGTG 1 cut(s) 406
AluBI AGCT 7 cut(s) 70, 205, 306, 340, 430, 488, 536
AluI AGCT 7 cut(s) 70, 205, 306, 340, 430, 488, 536
Alw21I GWGCWC 2 cut(s) 72, 562
Alw26I GTCTC 1 cut(s) 148
AlwI GGATC 3 cut(s) 68, 344, 357
AoxI GGCC 3 cut(s) 174, 195, 216
ApeKI GCWGC 2 cut(s) 485, 536
ApoI RAATTY 1 cut(s) 271
AspS9I GGNCC 3 cut(s) 211, 226, 330
AsuHPI GGTGA 3 cut(s) 154, 393, 435
AvaII GGWCC 3 cut(s) 211, 226, 330
AxyI CCTNAGG 1 cut(s) 375
BamHI GGATCC 1 cut(s) 349
BanII GRGCYC 1 cut(s) 72
Bbv12I GWGCWC 2 cut(s) 72, 562
BbvI GCAGC 2 cut(s) 497, 523
BccI CCATC 2 cut(s) 227, 260
BceAI ACGGC 3 cut(s) 161, 173, 189
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BciT130I CCWGG 2 cut(s) 215, 230
BciVI GTATCC 1 cut(s) 271
BclI TGATCA 1 cut(s) 39
BcoDI GTCTC 1 cut(s) 148
BfaI CTAG 1 cut(s) 470
BfuI GTATCC 1 cut(s) 271
BglI GCCNNNNNGGC 1 cut(s) 194
BisI GCNGC 3 cut(s) 186, 486, 537
BlsI GCNGC 3 cut(s) 187, 487, 538
Bme1390I CCNGG 2 cut(s) 215, 230
Bme18I GGWCC 3 cut(s) 211, 226, 330
BmgBI CACGTC 1 cut(s) 399
BmgT120I GGNCC 3 cut(s) 211, 226, 330
BmiI GGNNCC 2 cut(s) 331, 351
BmrFI CCNGG 2 cut(s) 215, 230
BmrI ACTGGG 1 cut(s) 54
BmuI ACTGGG 1 cut(s) 54
BoxI GACNNNNGTC 1 cut(s) 176
BsaBI GATNNNNATC 1 cut(s) 348
BsaHI GRCGYC 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 198
Bse1I ACTGG 2 cut(s) 49, 325
Bse21I CCTNAGG 1 cut(s) 375
Bse8I GATNNNNATC 1 cut(s) 348
BseBI CCWGG 2 cut(s) 215, 230
BseDI CCNNGG 1 cut(s) 198
BseGI GGATG 1 cut(s) 252
BseJI GATNNNNATC 1 cut(s) 348
BseMII CTCAG 1 cut(s) 323
BseNI ACTGG 2 cut(s) 49, 325
BseX3I CGGCCG 1 cut(s) 174
BseXI GCAGC 2 cut(s) 497, 523
BseYI CCCAGC 1 cut(s) 118
BsgI GTGCAG 1 cut(s) 339
Bsh1236I CGCG 1 cut(s) 237
Bsh1285I CGRYCG 1 cut(s) 177
BshFI GGCC 3 cut(s) 176, 197, 218
BsiEI CGRYCG 1 cut(s) 177
BsiHKAI GWGCWC 2 cut(s) 72, 562
BslFI GGGAC 1 cut(s) 343
BsmAI GTCTC 1 cut(s) 148
BsmBI CGTCTC 1 cut(s) 148
BsmFI GGGAC 1 cut(s) 343
BsnI GGCC 3 cut(s) 176, 197, 218
Bsp1286I GDGCHC 2 cut(s) 72, 562
Bsp143I GATC 3 cut(s) 39, 60, 349
Bsp19I CCATGG 1 cut(s) 198
Bsp68I TCGCGA 1 cut(s) 237
BspACI CCGC 1 cut(s) 186
BspANI GGCC 3 cut(s) 176, 197, 218
BspCNI CTCAG 1 cut(s) 322
BspFNI CGCG 1 cut(s) 237
BspLI GGNNCC 2 cut(s) 331, 351
BspPI GGATC 3 cut(s) 68, 344, 357
BspQI GCTCTTC 1 cut(s) 420
BsrI ACTGG 2 cut(s) 49, 325
BssECI CCNNGG 1 cut(s) 198
BssMI GATC 3 cut(s) 39, 60, 349
BssNI GRCGYC 1 cut(s) 183
BssT1I CCWWGG 1 cut(s) 198
Bst2UI CCWGG 2 cut(s) 215, 230
Bst4CI ACNGT 3 cut(s) 56, 98, 160
Bst6I CTCTTC 1 cut(s) 420
BstACI GRCGYC 1 cut(s) 183
BstAPI GCANNNNNTGC 1 cut(s) 509
BstDEI CTNAG 3 cut(s) 9, 309, 375
BstDSI CCRYGG 1 cut(s) 198
BstF5I GGATG 1 cut(s) 252
BstFNI CGCG 1 cut(s) 237
BstKTI GATC 3 cut(s) 42, 63, 352
BstMAI GTCTC 1 cut(s) 148
BstMBI GATC 3 cut(s) 39, 60, 349
BstMCI CGRYCG 1 cut(s) 177
BstMWI GCNNNNNNNGC 4 cut(s) 14, 182, 194, 509
BstNI CCWGG 2 cut(s) 215, 230
BstPAI GACNNNNGTC 1 cut(s) 176
BstSCI CCNGG 2 cut(s) 213, 228
BstUI CGCG 1 cut(s) 237
BstV1I GCAGC 2 cut(s) 497, 523
BstX2I RGATCY 2 cut(s) 60, 349
BstYI RGATCY 2 cut(s) 60, 349
BstZI CGGCCG 1 cut(s) 174
Bsu36I CCTNAGG 1 cut(s) 375
BsuI GTATCC 1 cut(s) 271
BsuRI GGCC 3 cut(s) 176, 197, 218
BtgI CCRYGG 1 cut(s) 198
BtrI CACGTC 1 cut(s) 399
BtsCI GGATG 1 cut(s) 252
BtsIMutI CAGTG 2 cut(s) 42, 318
BtuMI TCGCGA 1 cut(s) 237
Cfr13I GGNCC 3 cut(s) 211, 226, 330
CseI GACGC 2 cut(s) 12, 191
CsiI ACCWGGT 1 cut(s) 228
CviAII CATG 3 cut(s) 199, 451, 499
DdeI CTNAG 3 cut(s) 9, 309, 375
DpnI GATC 3 cut(s) 41, 62, 351
DpnII GATC 3 cut(s) 39, 60, 349
EaeI YGGCCR 1 cut(s) 174
EagI CGGCCG 1 cut(s) 174
Eam1104I CTCTTC 1 cut(s) 420
EarI CTCTTC 1 cut(s) 420
Ecl136II GAGCTC 1 cut(s) 70
EclXI CGGCCG 1 cut(s) 174
Eco130I CCWWGG 1 cut(s) 198
Eco24I GRGCYC 1 cut(s) 72
Eco47I GGWCC 3 cut(s) 211, 226, 330
Eco52I CGGCCG 1 cut(s) 174
Eco53kI GAGCTC 1 cut(s) 70
Eco57I CTGAAG 3 cut(s) 191, 444, 534
Eco81I CCTNAGG 1 cut(s) 375
EcoICRI GAGCTC 1 cut(s) 70
EcoO109I RGGNCCY 2 cut(s) 226, 330
EcoRII CCWGG 2 cut(s) 213, 228
EcoT14I CCWWGG 1 cut(s) 198
EcoT38I GRGCYC 1 cut(s) 72
ErhI CCWWGG 1 cut(s) 198
Esp3I CGTCTC 1 cut(s) 148
FaeI CATG 3 cut(s) 202, 454, 502
FaiI YATR 7 cut(s) 200, 416, 448, 452, 500, 508, 510
FaqI GGGAC 1 cut(s) 343
FatI CATG 3 cut(s) 198, 450, 498
FauNDI CATATG 1 cut(s) 508
FbaI TGATCA 1 cut(s) 39
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 3 cut(s) 186, 486, 537
FokI GGATG 1 cut(s) 239
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 3 cut(s) 186, 486, 537
FspBI CTAG 1 cut(s) 470
GluI GCNGC 3 cut(s) 186, 486, 537
GsaI CCCAGC 1 cut(s) 122
HaeIII GGCC 3 cut(s) 176, 197, 218
HgaI GACGC 2 cut(s) 12, 191
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 3 cut(s) 202, 454, 502
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 2 cut(s) 32, 434
HphI GGTGA 3 cut(s) 154, 393, 435
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 4 cut(s) 60, 67, 102, 572
Hpy188III TCNNGA 2 cut(s) 236, 299
Hpy8I GTNNAC 1 cut(s) 181
Hpy99I CGWCG 4 cut(s) 28, 182, 185, 242
HpyAV CCTTC 4 cut(s) 187, 343, 453, 469
HpyCH4III ACNGT 3 cut(s) 56, 98, 160
HpyCH4IV ACGT 1 cut(s) 398
HpyCH4V TGCA 2 cut(s) 356, 485
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 182, 194, 509
HpyF3I CTNAG 3 cut(s) 9, 309, 375
HpySE526I ACGT 1 cut(s) 398
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 3 cut(s) 202, 454, 502
Ksp22I TGATCA 1 cut(s) 39
Kzo9I GATC 3 cut(s) 39, 60, 349
LguI GCTCTTC 1 cut(s) 420
LmnI GCTCC 5 cut(s) 20, 67, 75, 202, 544
Lsp1109I GCAGC 2 cut(s) 497, 523
MabI ACCWGGT 1 cut(s) 228
MaeI CTAG 1 cut(s) 470
MaeII ACGT 1 cut(s) 398
MaeIII GTNAC 4 cut(s) 160, 399, 441, 494
MalI GATC 3 cut(s) 41, 62, 351
MboI GATC 3 cut(s) 39, 60, 349
MboII GAAGA 2 cut(s) 437, 559
MflI RGATCY 2 cut(s) 60, 349
MhlI GDGCHC 2 cut(s) 72, 562
MluCI AATT 5 cut(s) 131, 136, 266, 271, 520
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 2 cut(s) 9, 38
MnlI CCTC 3 cut(s) 257, 340, 449
MslI CAYNNNNRTG 1 cut(s) 406
MspR9I CCNGG 2 cut(s) 215, 230
MvaI CCWGG 2 cut(s) 215, 230
MvnI CGCG 1 cut(s) 237
MwoI GCNNNNNNNGC 4 cut(s) 14, 182, 194, 509
NcoI CCATGG 1 cut(s) 198
NdeI CATATG 1 cut(s) 508
NdeII GATC 3 cut(s) 39, 60, 349
NlaIII CATG 3 cut(s) 202, 454, 502
NlaIV GGNNCC 2 cut(s) 331, 351
NmuCI GTSAC 4 cut(s) 160, 399, 441, 494
NruI TCGCGA 1 cut(s) 237
OliI CACNNNNGTG 1 cut(s) 406
PciSI GCTCTTC 1 cut(s) 420
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PfeI GAWTC 1 cut(s) 434
PkrI GCNGC 3 cut(s) 187, 487, 538
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PpuMI RGGWCCY 2 cut(s) 226, 330
PshAI GACNNNNGTC 1 cut(s) 176
PsiI TTATAA 1 cut(s) 416
Psp124BI GAGCTC 1 cut(s) 72
Psp5II RGGWCCY 2 cut(s) 226, 330
Psp6I CCWGG 2 cut(s) 213, 228
PspFI CCCAGC 1 cut(s) 118
PspGI CCWGG 2 cut(s) 213, 228
PspN4I GGNNCC 2 cut(s) 331, 351
PspPI GGNCC 3 cut(s) 211, 226, 330
PspPPI RGGWCCY 2 cut(s) 226, 330
PsuI RGATCY 2 cut(s) 60, 349
RruI TCGCGA 1 cut(s) 237
RseI CAYNNNNRTG 1 cut(s) 406
SacI GAGCTC 1 cut(s) 72
SalI GTCGAC 1 cut(s) 179
SapI GCTCTTC 1 cut(s) 420
SatI GCNGC 3 cut(s) 186, 486, 537
Sau3AI GATC 3 cut(s) 39, 60, 349
Sau96I GGNCC 3 cut(s) 211, 226, 330
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 2 cut(s) 215, 230
SduI GDGCHC 2 cut(s) 72, 562
SexAI ACCWGGT 1 cut(s) 228
SgrDI CGTCGACG 1 cut(s) 179
SinI GGWCC 3 cut(s) 211, 226, 330
SmiMI CAYNNNNRTG 1 cut(s) 406
Sse9I AATT 5 cut(s) 131, 136, 266, 271, 520
SsiI CCGC 1 cut(s) 186
SspMI CTAG 1 cut(s) 470
SstI GAGCTC 1 cut(s) 72
StyD4I CCNGG 2 cut(s) 213, 228
StyI CCWWGG 1 cut(s) 198
TaaI ACNGT 3 cut(s) 56, 98, 160
TaiI ACGT 1 cut(s) 401
TaqI TCGA 2 cut(s) 91, 180
TasI AATT 5 cut(s) 131, 136, 266, 271, 520
TauI GCSGC 1 cut(s) 188
TfiI GAWTC 1 cut(s) 434
TscAI CASTG 2 cut(s) 49, 325
TseFI GTSAC 4 cut(s) 160, 399, 441, 494
TseI GCWGC 2 cut(s) 485, 536
Tsp45I GTSAC 4 cut(s) 160, 399, 441, 494
TspRI CASTG 2 cut(s) 49, 325
VpaK11BI GGWCC 3 cut(s) 211, 226, 330
XapI RAATTY 1 cut(s) 271
XcmI CCANNNNNNNNNTGG 1 cut(s) 226
XmiI GTMKAC 1 cut(s) 180
XspI CTAG 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.