RLG00000028571

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001324
Physical Location & Seq
Reverse (-)
24493467 .. 24496775
3309 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028571

Sequence Viewer

Length: 702 bp
ATGGAGGCTGAGAAAGCAGGGGCATCGTTGGACTCGCTGATTGCCGGGTTCAACAGTCGGATCTCGGAGCTCCAAGAGCTCGTTATTGCGCGAAACATGTACCCGGCCAGCAGCGTCACCGATTTAACGGCCGTCGACGCCGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGCCATCAAGAACCGGGTTCGCGACGAAACCCTAGCCATCCCCAAAGCAAAAAAACTGATCGATGCATCGCTGAAGCAGCAAAGGAAATTGAAGGATATGTCAATTTATGCCCCCACTCATGTTCCTGAAAGAATGTCGATGTTGAATTTGGATACAAATAGATGTTTATTTCCAGAAAGCTCTCAGCAAAACACTGGTTCTGGGACCTTCAAGCTTGAGGAGGATCCTGCACCATCACCACCCAAGGAGAAAAGGGGTCGTTCCACGCCACCATTGTGGTTTGTAAGTGCTGAAGAGCTGGATTCCTTGTCGTCGTACATGAGAGGAAGGCTTACACTAGAGAAAGTGAATGCAGCTATTAATGACATGGCAACATATGCTGAAGCAAATTATCAGCTTATAGCTGCTCCCAAGAAGAATGTGGCAGGAGACCGTTGGGAAAAGGCTCTGGGGGAAGTTGATCCTGAAAATAGATTTAGGATACTCCATGCTGTTCTTTACACAGCAGAGGGGAAAATTGGTTCATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

234

Amino Acids

25.79

Weight (kDa)

6.77

Isoelectric Point (pI)

47.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 9 - 207 7.7e-56 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 135
AccII CGCG 2 cut(s) 91, 192
AciI CCGC 1 cut(s) 141
AclWI GGATC 4 cut(s) 68, 389, 402, 626
AcoI YGGCCR 2 cut(s) 105, 129
AcsI RAATTY 1 cut(s) 316
AcuI CTGAAG 4 cut(s) 146, 263, 483, 573
AcyI GRCGYC 1 cut(s) 138
AfaI GTAC 2 cut(s) 101, 488
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 4 cut(s) 52, 262, 316, 382
AjnI CCWGG 1 cut(s) 168
AleI CACNNNNGTG 1 cut(s) 445
AloI GAACNNNNNNTCC 1 cut(s) 676
AluBI AGCT 9 cut(s) 70, 79, 160, 351, 385, 469, 527, 568, 575
AluI AGCT 9 cut(s) 70, 79, 160, 351, 385, 469, 527, 568, 575
Alw21I GWGCWC 2 cut(s) 72, 81
Alw26I GTCTC 1 cut(s) 594
AlwI GGATC 4 cut(s) 68, 389, 402, 626
AoxI GGCC 4 cut(s) 105, 129, 150, 171
ApeKI GCWGC 4 cut(s) 111, 247, 524, 575
ApoI RAATTY 1 cut(s) 316
AseI ATTAAT 1 cut(s) 531
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 2 cut(s) 166, 375
AsuC2I CCSGG 3 cut(s) 46, 104, 185
AsuHPI GGTGA 2 cut(s) 109, 399
AvaII GGWCC 2 cut(s) 166, 375
BamHI GGATCC 1 cut(s) 394
BanII GRGCYC 2 cut(s) 72, 81
Bbv12I GWGCWC 2 cut(s) 72, 81
BbvI GCAGC 4 cut(s) 123, 259, 536, 562
BccI CCATC 3 cut(s) 182, 215, 412
BceAI ACGGC 3 cut(s) 116, 128, 144
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BciT130I CCWGG 1 cut(s) 170
BciVI GTATCC 2 cut(s) 316, 645
BcnI CCSGG 3 cut(s) 46, 104, 185
BcoDI GTCTC 1 cut(s) 594
BfaI CTAG 2 cut(s) 203, 509
BfuI GTATCC 2 cut(s) 316, 645
BglI GCCNNNNNGGC 1 cut(s) 149
BisI GCNGC 5 cut(s) 112, 141, 248, 525, 576
BlsI GCNGC 5 cut(s) 113, 142, 249, 526, 577
Bme1390I CCNGG 4 cut(s) 46, 104, 170, 185
Bme18I GGWCC 2 cut(s) 166, 375
BmgT120I GGNCC 2 cut(s) 166, 375
BmiI GGNNCC 2 cut(s) 376, 396
BmrFI CCNGG 4 cut(s) 46, 104, 170, 185
BmsI GCATC 3 cut(s) 32, 223, 245
BpuEI CTTGAG 1 cut(s) 407
BpuMI CCSGG 3 cut(s) 46, 104, 185
Bsa29I ATCGAT 1 cut(s) 231
BsaHI GRCGYC 1 cut(s) 138
BsaI GGTCTC 1 cut(s) 594
BsaJI CCNNGG 2 cut(s) 153, 414
Bse1I ACTGG 1 cut(s) 370
BseBI CCWGG 1 cut(s) 170
BseCI ATCGAT 1 cut(s) 231
BseDI CCNNGG 2 cut(s) 153, 414
BseGI GGATG 1 cut(s) 207
BseMII CTCAG 1 cut(s) 368
BseNI ACTGG 1 cut(s) 370
BseRI GAGGAG 1 cut(s) 404
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 4 cut(s) 123, 259, 536, 562
BsgI GTGCAG 1 cut(s) 384
Bsh1236I CGCG 2 cut(s) 91, 192
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 4 cut(s) 107, 131, 152, 173
BshVI ATCGAT 1 cut(s) 231
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 2 cut(s) 72, 81
BsiSI CCGG 3 cut(s) 45, 104, 184
BslFI GGGAC 1 cut(s) 388
BsmAI GTCTC 1 cut(s) 594
BsmFI GGGAC 1 cut(s) 388
BsmI GAATGC 1 cut(s) 526
BsnI GGCC 4 cut(s) 107, 131, 152, 173
Bso31I GGTCTC 1 cut(s) 594
Bsp1286I GDGCHC 2 cut(s) 72, 81
Bsp143I GATC 4 cut(s) 60, 228, 394, 631
Bsp19I CCATGG 1 cut(s) 153
Bsp68I TCGCGA 1 cut(s) 192
BspACI CCGC 1 cut(s) 141
BspANI GGCC 4 cut(s) 107, 131, 152, 173
BspCNI CTCAG 1 cut(s) 367
BspDI ATCGAT 1 cut(s) 231
BspFNI CGCG 2 cut(s) 91, 192
BspLI GGNNCC 2 cut(s) 376, 396
BspPI GGATC 4 cut(s) 68, 389, 402, 626
BspQI GCTCTTC 1 cut(s) 459
BspTNI GGTCTC 1 cut(s) 594
BsrI ACTGG 1 cut(s) 370
BssECI CCNNGG 2 cut(s) 153, 414
BssMI GATC 4 cut(s) 60, 228, 394, 631
BssNI GRCGYC 1 cut(s) 138
BssT1I CCWWGG 2 cut(s) 153, 414
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 2 cut(s) 56, 605
Bst6I CTCTTC 1 cut(s) 459
BstACI GRCGYC 1 cut(s) 138
BstAPI GCANNNNNTGC 1 cut(s) 548
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 2 cut(s) 9, 354
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 1 cut(s) 207
BstFNI CGCG 2 cut(s) 91, 192
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 4 cut(s) 63, 231, 397, 634
BstMAI GTCTC 1 cut(s) 594
BstMBI GATC 4 cut(s) 60, 228, 394, 631
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 6 cut(s) 14, 76, 137, 149, 247, 548
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 100
BstSCI CCNGG 4 cut(s) 44, 102, 168, 183
BstUI CGCG 2 cut(s) 91, 192
BstV1I GCAGC 4 cut(s) 123, 259, 536, 562
BstX2I RGATCY 2 cut(s) 60, 394
BstXI CCANNNNNNTGG 1 cut(s) 447
BstYI RGATCY 2 cut(s) 60, 394
BstZI CGGCCG 1 cut(s) 129
Bsu15I ATCGAT 1 cut(s) 231
BsuI GTATCC 2 cut(s) 316, 645
BsuRI GGCC 4 cut(s) 107, 131, 152, 173
BsuTUI ATCGAT 1 cut(s) 231
BtgI CCRYGG 1 cut(s) 153
BtgZI GCGATG 1 cut(s) 222
BtsCI GGATG 1 cut(s) 207
BtsIMutI CAGTG 1 cut(s) 363
BtuMI TCGCGA 1 cut(s) 192
Cac8I GCNNGC 1 cut(s) 109
CfoI GCGC 1 cut(s) 91
Cfr13I GGNCC 2 cut(s) 166, 375
ClaI ATCGAT 1 cut(s) 231
CseI GACGC 2 cut(s) 103, 146
Csp6I GTAC 2 cut(s) 100, 487
CviAII CATG 6 cut(s) 97, 154, 290, 490, 538, 659
CviQI GTAC 2 cut(s) 100, 487
DdeI CTNAG 2 cut(s) 9, 354
DpnI GATC 4 cut(s) 62, 230, 396, 633
DpnII GATC 4 cut(s) 60, 228, 394, 631
EaeI YGGCCR 2 cut(s) 105, 129
EagI CGGCCG 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 459
EarI CTCTTC 1 cut(s) 459
Ecl136II GAGCTC 2 cut(s) 70, 79
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 2 cut(s) 153, 414
Eco24I GRGCYC 2 cut(s) 72, 81
Eco31I GGTCTC 1 cut(s) 594
Eco47I GGWCC 2 cut(s) 166, 375
Eco52I CGGCCG 1 cut(s) 129
Eco53kI GAGCTC 2 cut(s) 70, 79
Eco57I CTGAAG 4 cut(s) 146, 263, 483, 573
EcoICRI GAGCTC 2 cut(s) 70, 79
EcoO109I RGGNCCY 1 cut(s) 375
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 2 cut(s) 153, 414
EcoT22I ATGCAT 1 cut(s) 238
EcoT38I GRGCYC 2 cut(s) 72, 81
ErhI CCWWGG 2 cut(s) 153, 414
FaeI CATG 6 cut(s) 100, 157, 293, 493, 541, 662
FaqI GGGAC 1 cut(s) 388
FatI CATG 6 cut(s) 96, 153, 289, 489, 537, 658
FauNDI CATATG 1 cut(s) 547
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 5 cut(s) 112, 141, 248, 525, 576
FokI GGATG 1 cut(s) 194
FriOI GRGCYC 2 cut(s) 72, 81
Fsp4HI GCNGC 5 cut(s) 112, 141, 248, 525, 576
FspBI CTAG 2 cut(s) 203, 509
GlaI GCGC 1 cut(s) 90
GluI GCNGC 5 cut(s) 112, 141, 248, 525, 576
HaeIII GGCC 4 cut(s) 107, 131, 152, 173
HapII CCGG 3 cut(s) 45, 104, 184
HgaI GACGC 2 cut(s) 103, 146
HhaI GCGC 1 cut(s) 91
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 6 cut(s) 100, 157, 293, 493, 541, 662
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 2 cut(s) 32, 473
HpaII CCGG 3 cut(s) 45, 104, 184
HphI GGTGA 2 cut(s) 109, 399
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 2 cut(s) 60, 67
Hpy188III TCNNGA 5 cut(s) 178, 191, 296, 344, 635
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 4 cut(s) 137, 140, 197, 487
HpyAV CCTTC 4 cut(s) 142, 256, 388, 492
HpyCH4III ACNGT 2 cut(s) 56, 605
HpyCH4V TGCA 3 cut(s) 236, 401, 524
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 76, 137, 149, 247, 548
HpyF3I CTNAG 2 cut(s) 9, 354
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 6 cut(s) 100, 157, 293, 493, 541, 662
HspAI GCGC 1 cut(s) 89
Kzo9I GATC 4 cut(s) 60, 228, 394, 631
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 4 cut(s) 67, 75, 157, 583
Lsp1109I GCAGC 4 cut(s) 123, 259, 536, 562
LweI GCATC 3 cut(s) 32, 223, 245
MaeI CTAG 2 cut(s) 203, 509
MaeIII GTNAC 1 cut(s) 115
MalI GATC 4 cut(s) 62, 230, 396, 633
MboI GATC 4 cut(s) 60, 228, 394, 631
MboII GAAGA 2 cut(s) 476, 598
MflI RGATCY 2 cut(s) 60, 394
MhlI GDGCHC 2 cut(s) 72, 81
MluCI AATT 5 cut(s) 257, 273, 316, 559, 687
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 2 cut(s) 9, 38
MnlI CCTC 4 cut(s) 382, 385, 488, 673
Mph1103I ATGCAT 1 cut(s) 238
MseI TTAA 2 cut(s) 125, 531
MslI CAYNNNNRTG 1 cut(s) 445
MspI CCGG 3 cut(s) 45, 104, 184
MspR9I CCNGG 4 cut(s) 46, 104, 170, 185
Mva1269I GAATGC 1 cut(s) 526
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 2 cut(s) 91, 192
MwoI GCNNNNNNNGC 6 cut(s) 14, 76, 137, 149, 247, 548
NciI CCSGG 3 cut(s) 46, 104, 185
NcoI CCATGG 1 cut(s) 153
NdeI CATATG 1 cut(s) 547
NdeII GATC 4 cut(s) 60, 228, 394, 631
NlaIII CATG 6 cut(s) 100, 157, 293, 493, 541, 662
NlaIV GGNNCC 2 cut(s) 376, 396
NmuCI GTSAC 1 cut(s) 115
NruI TCGCGA 1 cut(s) 192
NsiI ATGCAT 1 cut(s) 238
NspI RCATGY 1 cut(s) 100
OliI CACNNNNGTG 1 cut(s) 445
PciI ACATGT 1 cut(s) 96
PciSI GCTCTTC 1 cut(s) 459
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PctI GAATGC 1 cut(s) 526
PfeI GAWTC 1 cut(s) 473
PkrI GCNGC 5 cut(s) 113, 142, 249, 526, 577
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PpuMI RGGWCCY 1 cut(s) 375
PscI ACATGT 1 cut(s) 96
PshBI ATTAAT 1 cut(s) 531
Psp124BI GAGCTC 2 cut(s) 72, 81
Psp5II RGGWCCY 1 cut(s) 375
Psp6I CCWGG 1 cut(s) 168
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 2 cut(s) 376, 396
PspPI GGNCC 2 cut(s) 166, 375
PspPPI RGGWCCY 1 cut(s) 375
PsuI RGATCY 2 cut(s) 60, 394
RruI TCGCGA 1 cut(s) 192
RsaI GTAC 2 cut(s) 101, 488
RsaNI GTAC 2 cut(s) 100, 487
RseI CAYNNNNRTG 1 cut(s) 445
SacI GAGCTC 2 cut(s) 72, 81
SalI GTCGAC 1 cut(s) 134
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 2 cut(s) 125, 531
SatI GCNGC 5 cut(s) 112, 141, 248, 525, 576
Sau3AI GATC 4 cut(s) 60, 228, 394, 631
Sau96I GGNCC 2 cut(s) 166, 375
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 4 cut(s) 46, 104, 170, 185
SduI GDGCHC 2 cut(s) 72, 81
SfaNI GCATC 3 cut(s) 32, 223, 245
SgrDI CGTCGACG 1 cut(s) 134
SinI GGWCC 2 cut(s) 166, 375
SmiMI CAYNNNNRTG 1 cut(s) 445
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
Sse9I AATT 5 cut(s) 257, 273, 316, 559, 687
SsiI CCGC 1 cut(s) 141
SspMI CTAG 2 cut(s) 203, 509
SstI GAGCTC 2 cut(s) 72, 81
StyD4I CCNGG 4 cut(s) 44, 102, 168, 183
StyI CCWWGG 2 cut(s) 153, 414
TaaI ACNGT 2 cut(s) 56, 605
TaqI TCGA 3 cut(s) 135, 231, 308
TasI AATT 5 cut(s) 257, 273, 316, 559, 687
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 1 cut(s) 473
Tru1I TTAA 2 cut(s) 125, 531
Tru9I TTAA 2 cut(s) 125, 531
TscAI CASTG 1 cut(s) 370
TseFI GTSAC 1 cut(s) 115
TseI GCWGC 4 cut(s) 111, 247, 524, 575
Tsp45I GTSAC 1 cut(s) 115
TspDTI ATGAA 1 cut(s) 684
TspRI CASTG 1 cut(s) 370
VpaK11BI GGWCC 2 cut(s) 166, 375
VspI ATTAAT 1 cut(s) 531
XapI RAATTY 1 cut(s) 316
XceI RCATGY 1 cut(s) 100
XcmI CCANNNNNNNNNTGG 1 cut(s) 589
XmiI GTMKAC 1 cut(s) 135
XspI CTAG 2 cut(s) 203, 509
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.