RchiOBHm_Chr5g0028911

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001324
Physical Location & Seq
Reverse (-)
22688588 .. 22692202
3615 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30832

Sequence Viewer

Length: 825 bp
ATGGAGGCGGAGAAAGCAGGGGCGTCGTTGGACTCGCTGATCGCCGGGTTCAACAGTCGGATCTCGGAGCTCAAAGAACTCGTTATTGCGCGAAACATGTACCCAGCCAGCAGCGTCACCGATTTGTCGGCCGTCGACGCCGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGCCATCAAGGACCGGGTTGGCGACGAAACCCTAGCCATCCCCAAAGCAAAAAAACTGATCGATGCATCGCTGAAGCAGCAAAGGAAATTGAAGGATATGTCAGTTTATGCCCCCACTCATGTTCCTGAAAGGATGTCAATGTTGAATTTGGATACAAACAGATGTTTATTTCCAGAAAGCTCTCAGCAAAACACTGGTTCTGGGACCTTCAATCTTGACGAGGATCCTGCACCACCACCACCACCACCACCACCAAAGGAGAAAAAGGGTCGTTCCACACCACCATTGTGGTTTATAAGTACTGAAGAGTTGGATTCCTTGTCATCATACATGAGAGGAAGGCTTACACTAGAGAAGGTCAATGCAGCTATTAATGACATGGCAACATATGCTGAAGCAAATTATCAGCTCATAGCTGCTCCAAAGAAGAATGTGGCAGGGGATCGATGGGAAAAAGCCTTGGAAATAAGAGATATAGCAATGCATGAAGCAGTAAAGGGAAAACACTTCTTTCTCGAAACTGACATGAAGGGACCATCCTTGAAGCTTGACAACACTGGAAAAGCAATACTAACTGTCCTTCGTCACCTTGGTCGGGTAAATGAGACACGGATTGGTCATCATCGGGTGATCATTCTTTTGAAGCCTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

274

Amino Acids

30.33

Weight (kDa)

8.79

Isoelectric Point (pI)

43.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 9 - 264 1.7e-85 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 467
AccI GTMKAC 1 cut(s) 135
AccII CGCG 1 cut(s) 91
AciI CCGC 2 cut(s) 8, 141
AclWI GGATC 4 cut(s) 68, 389, 402, 621
AcoI YGGCCR 1 cut(s) 129
AcsI RAATTY 1 cut(s) 316
AcuI CTGAAG 4 cut(s) 146, 263, 495, 585
AcyI GRCGYC 2 cut(s) 23, 138
AfaI GTAC 2 cut(s) 101, 472
AfiI CCNNNNNNNGG 1 cut(s) 766
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 6 cut(s) 52, 262, 316, 382, 715, 814
AjnI CCWGG 1 cut(s) 168
AleI CACNNNNGTG 1 cut(s) 457
AluBI AGCT 7 cut(s) 70, 160, 351, 539, 580, 587, 718
AluI AGCT 7 cut(s) 70, 160, 351, 539, 580, 587, 718
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 1 cut(s) 770
AlwI GGATC 4 cut(s) 68, 389, 402, 621
AoxI GGCC 3 cut(s) 129, 150, 171
ApeKI GCWGC 4 cut(s) 111, 247, 536, 587
ApoI RAATTY 1 cut(s) 316
AseI ATTAAT 1 cut(s) 543
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 4 cut(s) 166, 181, 375, 704
AsuC2I CCSGG 2 cut(s) 46, 185
AsuHPI GGTGA 3 cut(s) 109, 749, 811
AvaII GGWCC 4 cut(s) 166, 181, 375, 704
BamHI GGATCC 1 cut(s) 394
BanII GRGCYC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 72
BbvI GCAGC 4 cut(s) 123, 259, 548, 574
BccI CCATC 4 cut(s) 182, 215, 612, 715
BceAI ACGGC 2 cut(s) 116, 128
BcgI CGANNNNNNTGC 4 cut(s) 6, 40, 380, 414
BciT130I CCWGG 1 cut(s) 170
BciVI GTATCC 1 cut(s) 316
BclI TGATCA 1 cut(s) 801
BcnI CCSGG 2 cut(s) 46, 185
BcoDI GTCTC 1 cut(s) 770
BfaI CTAG 2 cut(s) 203, 521
BfuI GTATCC 1 cut(s) 316
BglI GCCNNNNNGGC 1 cut(s) 149
BisI GCNGC 5 cut(s) 112, 141, 248, 537, 588
BlsI GCNGC 5 cut(s) 113, 142, 249, 538, 589
BmcAI AGTACT 1 cut(s) 472
Bme1390I CCNGG 3 cut(s) 46, 170, 185
Bme18I GGWCC 4 cut(s) 166, 181, 375, 704
BmgT120I GGNCC 4 cut(s) 166, 181, 375, 704
BmiI GGNNCC 3 cut(s) 376, 396, 705
BmrFI CCNGG 3 cut(s) 46, 170, 185
BmsI GCATC 2 cut(s) 223, 245
BpuMI CCSGG 2 cut(s) 46, 185
Bsa29I ATCGAT 2 cut(s) 231, 616
BsaHI GRCGYC 2 cut(s) 23, 138
BsaJI CCNNGG 3 cut(s) 153, 630, 760
Bsc4I CCNNNNNNNGG 1 cut(s) 766
Bse1I ACTGG 2 cut(s) 370, 733
Bse3DI GCAATG 1 cut(s) 657
BseBI CCWGG 1 cut(s) 170
BseCI ATCGAT 2 cut(s) 231, 616
BseDI CCNNGG 3 cut(s) 153, 630, 760
BseGI GGATG 3 cut(s) 207, 309, 707
BseLI CCNNNNNNNGG 1 cut(s) 766
BseMI GCAATG 1 cut(s) 657
BseMII CTCAG 1 cut(s) 368
BseNI ACTGG 2 cut(s) 370, 733
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 4 cut(s) 123, 259, 548, 574
BseYI CCCAGC 1 cut(s) 103
BsgI GTGCAG 1 cut(s) 384
Bsh1236I CGCG 1 cut(s) 91
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 3 cut(s) 131, 152, 173
BshVI ATCGAT 2 cut(s) 231, 616
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 72
BsiSI CCGG 2 cut(s) 45, 184
BslFI GGGAC 2 cut(s) 388, 717
BslI CCNNNNNNNGG 1 cut(s) 766
BsmAI GTCTC 1 cut(s) 770
BsmFI GGGAC 2 cut(s) 388, 717
BsnI GGCC 3 cut(s) 131, 152, 173
Bsp1286I GDGCHC 1 cut(s) 72
Bsp143I GATC 6 cut(s) 39, 60, 228, 394, 613, 801
Bsp19I CCATGG 1 cut(s) 153
BspACI CCGC 2 cut(s) 8, 141
BspANI GGCC 3 cut(s) 131, 152, 173
BspCNI CTCAG 1 cut(s) 367
BspDI ATCGAT 2 cut(s) 231, 616
BspFNI CGCG 1 cut(s) 91
BspLI GGNNCC 3 cut(s) 376, 396, 705
BspPI GGATC 4 cut(s) 68, 389, 402, 621
BsrDI GCAATG 1 cut(s) 657
BsrI ACTGG 2 cut(s) 370, 733
BssECI CCNNGG 3 cut(s) 153, 630, 760
BssMI GATC 6 cut(s) 39, 60, 228, 394, 613, 801
BssNI GRCGYC 2 cut(s) 23, 138
BssT1I CCWWGG 3 cut(s) 153, 630, 760
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 2 cut(s) 56, 748
Bst6I CTCTTC 1 cut(s) 471
BstACI GRCGYC 2 cut(s) 23, 138
BstAPI GCANNNNNTGC 1 cut(s) 560
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 1 cut(s) 354
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 3 cut(s) 207, 309, 707
BstFNI CGCG 1 cut(s) 91
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 6 cut(s) 42, 63, 231, 397, 616, 804
BstMAI GTCTC 1 cut(s) 770
BstMBI GATC 6 cut(s) 39, 60, 228, 394, 613, 801
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 5 cut(s) 14, 137, 149, 247, 560
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 100
BstSCI CCNGG 3 cut(s) 44, 168, 183
BstUI CGCG 1 cut(s) 91
BstV1I GCAGC 4 cut(s) 123, 259, 548, 574
BstX2I RGATCY 2 cut(s) 60, 394
BstXI CCANNNNNNTGG 1 cut(s) 459
BstYI RGATCY 2 cut(s) 60, 394
BstZI CGGCCG 1 cut(s) 129
Bsu15I ATCGAT 2 cut(s) 231, 616
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 3 cut(s) 131, 152, 173
BsuTUI ATCGAT 2 cut(s) 231, 616
BtgI CCRYGG 1 cut(s) 153
BtgZI GCGATG 1 cut(s) 222
BtsCI GGATG 3 cut(s) 207, 309, 707
BtsIMutI CAGTG 3 cut(s) 363, 726, 820
Cac8I GCNNGC 1 cut(s) 109
CfoI GCGC 1 cut(s) 91
Cfr13I GGNCC 4 cut(s) 166, 181, 375, 704
ClaI ATCGAT 2 cut(s) 231, 616
CseI GACGC 3 cut(s) 12, 103, 146
Csp6I GTAC 2 cut(s) 100, 471
CviAII CATG 7 cut(s) 97, 154, 290, 502, 550, 656, 697
CviQI GTAC 2 cut(s) 100, 471
DdeI CTNAG 1 cut(s) 354
DpnI GATC 6 cut(s) 41, 62, 230, 396, 615, 803
DpnII GATC 6 cut(s) 39, 60, 228, 394, 613, 801
EaeI YGGCCR 1 cut(s) 129
EagI CGGCCG 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 471
EarI CTCTTC 1 cut(s) 471
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 1 cut(s) 70
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 3 cut(s) 153, 630, 760
Eco24I GRGCYC 1 cut(s) 72
Eco47I GGWCC 4 cut(s) 166, 181, 375, 704
Eco52I CGGCCG 1 cut(s) 129
Eco53kI GAGCTC 1 cut(s) 70
Eco57I CTGAAG 4 cut(s) 146, 263, 495, 585
EcoICRI GAGCTC 1 cut(s) 70
EcoO109I RGGNCCY 1 cut(s) 375
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 3 cut(s) 153, 630, 760
EcoT22I ATGCAT 2 cut(s) 238, 657
EcoT38I GRGCYC 1 cut(s) 72
ErhI CCWWGG 3 cut(s) 153, 630, 760
FaeI CATG 7 cut(s) 100, 157, 293, 505, 553, 659, 700
FaqI GGGAC 2 cut(s) 388, 717
FatI CATG 7 cut(s) 96, 153, 289, 501, 549, 655, 696
FauNDI CATATG 1 cut(s) 559
FbaI TGATCA 1 cut(s) 801
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 5 cut(s) 112, 141, 248, 537, 588
FokI GGATG 3 cut(s) 194, 316, 694
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 5 cut(s) 112, 141, 248, 537, 588
FspBI CTAG 2 cut(s) 203, 521
GlaI GCGC 1 cut(s) 90
GluI GCNGC 5 cut(s) 112, 141, 248, 537, 588
GsaI CCCAGC 1 cut(s) 107
HaeIII GGCC 3 cut(s) 131, 152, 173
HapII CCGG 2 cut(s) 45, 184
HgaI GACGC 3 cut(s) 12, 103, 146
HhaI GCGC 1 cut(s) 91
Hin1I GRCGYC 2 cut(s) 23, 138
Hin1II CATG 7 cut(s) 100, 157, 293, 505, 553, 659, 700
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HindIII AAGCTT 1 cut(s) 716
HinfI GANTC 2 cut(s) 32, 485
HpaII CCGG 2 cut(s) 45, 184
HphI GGTGA 3 cut(s) 109, 749, 811
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 2 cut(s) 60, 67
Hpy188III TCNNGA 4 cut(s) 296, 344, 386, 686
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 4 cut(s) 28, 137, 140, 197
HpyAV CCTTC 7 cut(s) 142, 256, 388, 504, 520, 694, 761
HpyCH4III ACNGT 2 cut(s) 56, 748
HpyCH4V TGCA 4 cut(s) 236, 401, 536, 655
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 137, 149, 247, 560
HpyF3I CTNAG 1 cut(s) 354
Hsp92I GRCGYC 2 cut(s) 23, 138
Hsp92II CATG 7 cut(s) 100, 157, 293, 505, 553, 659, 700
HspAI GCGC 1 cut(s) 89
Ksp22I TGATCA 1 cut(s) 801
Kzo9I GATC 6 cut(s) 39, 60, 228, 394, 613, 801
LmnI GCTCC 3 cut(s) 67, 157, 595
Lsp1109I GCAGC 4 cut(s) 123, 259, 548, 574
LweI GCATC 2 cut(s) 223, 245
MaeI CTAG 2 cut(s) 203, 521
MaeIII GTNAC 2 cut(s) 115, 755
MalI GATC 6 cut(s) 41, 62, 230, 396, 615, 803
MboI GATC 6 cut(s) 39, 60, 228, 394, 613, 801
MboII GAAGA 2 cut(s) 488, 610
MflI RGATCY 2 cut(s) 60, 394
MhlI GDGCHC 1 cut(s) 72
MluCI AATT 3 cut(s) 257, 316, 571
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 3 cut(s) 9, 38, 462
MnlI CCTC 2 cut(s) 385, 500
Mph1103I ATGCAT 2 cut(s) 238, 657
MseI TTAA 1 cut(s) 543
MslI CAYNNNNRTG 1 cut(s) 457
MspI CCGG 2 cut(s) 45, 184
MspR9I CCNGG 3 cut(s) 46, 170, 185
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 1 cut(s) 91
MwoI GCNNNNNNNGC 5 cut(s) 14, 137, 149, 247, 560
NciI CCSGG 2 cut(s) 46, 185
NcoI CCATGG 1 cut(s) 153
NdeI CATATG 1 cut(s) 559
NdeII GATC 6 cut(s) 39, 60, 228, 394, 613, 801
NlaIII CATG 7 cut(s) 100, 157, 293, 505, 553, 659, 700
NlaIV GGNNCC 3 cut(s) 376, 396, 705
NmuCI GTSAC 2 cut(s) 115, 755
NsiI ATGCAT 2 cut(s) 238, 657
NspI RCATGY 1 cut(s) 100
OliI CACNNNNGTG 1 cut(s) 457
PciI ACATGT 1 cut(s) 96
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PfeI GAWTC 1 cut(s) 485
PkrI GCNGC 5 cut(s) 113, 142, 249, 538, 589
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PpuMI RGGWCCY 1 cut(s) 375
PscI ACATGT 1 cut(s) 96
PshBI ATTAAT 1 cut(s) 543
PsiI TTATAA 1 cut(s) 467
Psp124BI GAGCTC 1 cut(s) 72
Psp5II RGGWCCY 1 cut(s) 375
Psp6I CCWGG 1 cut(s) 168
PspFI CCCAGC 1 cut(s) 103
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 3 cut(s) 376, 396, 705
PspPI GGNCC 4 cut(s) 166, 181, 375, 704
PspPPI RGGWCCY 1 cut(s) 375
PsuI RGATCY 2 cut(s) 60, 394
RsaI GTAC 2 cut(s) 101, 472
RsaNI GTAC 2 cut(s) 100, 471
RseI CAYNNNNRTG 1 cut(s) 457
SacI GAGCTC 1 cut(s) 72
SalI GTCGAC 1 cut(s) 134
SaqAI TTAA 1 cut(s) 543
SatI GCNGC 5 cut(s) 112, 141, 248, 537, 588
Sau3AI GATC 6 cut(s) 39, 60, 228, 394, 613, 801
Sau96I GGNCC 4 cut(s) 166, 181, 375, 704
ScaI AGTACT 1 cut(s) 472
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 3 cut(s) 46, 170, 185
SduI GDGCHC 1 cut(s) 72
SfaNI GCATC 2 cut(s) 223, 245
SgrDI CGTCGACG 1 cut(s) 134
SinI GGWCC 4 cut(s) 166, 181, 375, 704
SmiMI CAYNNNNRTG 1 cut(s) 457
Sse9I AATT 3 cut(s) 257, 316, 571
SsiI CCGC 2 cut(s) 8, 141
SspMI CTAG 2 cut(s) 203, 521
SstI GAGCTC 1 cut(s) 72
StyD4I CCNGG 3 cut(s) 44, 168, 183
StyI CCWWGG 3 cut(s) 153, 630, 760
TaaI ACNGT 2 cut(s) 56, 748
TaqI TCGA 4 cut(s) 135, 231, 616, 687
TasI AATT 3 cut(s) 257, 316, 571
TatI WGTACW 1 cut(s) 470
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 1 cut(s) 485
Tru1I TTAA 1 cut(s) 543
Tru9I TTAA 1 cut(s) 543
TscAI CASTG 2 cut(s) 370, 733
TseFI GTSAC 2 cut(s) 115, 755
TseI GCWGC 4 cut(s) 111, 247, 536, 587
Tsp45I GTSAC 2 cut(s) 115, 755
TspDTI ATGAA 2 cut(s) 672, 713
TspGWI ACGGA 1 cut(s) 796
TspRI CASTG 2 cut(s) 370, 733
VpaK11BI GGWCC 4 cut(s) 166, 181, 375, 704
VspI ATTAAT 1 cut(s) 543
XapI RAATTY 1 cut(s) 316
XceI RCATGY 1 cut(s) 100
XmiI GTMKAC 1 cut(s) 135
XspI CTAG 2 cut(s) 203, 521
ZrmI AGTACT 1 cut(s) 472
Zsp2I ATGCAT 2 cut(s) 238, 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.