Rroxscaffold_4G00306010

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001324
Physical Location & Seq
Reverse (-)
26803068 .. 26806623
3556 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00306010.1

Sequence Viewer

Length: 831 bp
ATGCTTCTCATTTTCCCGCCAAAACAACTTTCCCAACTTCAAAACGAAAATCCACGAATCGAAGATCGAAACTCTCTGAAGAGAGAGAGGGAGAGAGCGATGGAGGCAGAGAAAGCAGGGGCGTCGTTGGACTCGCTGATCGCCGGGTTCAACAGTCGGGTCTCGGAGCTCCAAGGGCTTGTTATTGCGCGAAACATGTACCCGGCCAGCAGCGTCACCGATTTGACGGCCGTCGACGCAGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGCCATCAAGGACCGGGTTCGCGACGAAACCCTAGCTATCCCCAAAGCAAAAGTATGTGCGAGATGGTACAATGTGATGGCAGAAACTGATCGATGCATTGCTGAAGCAACAAAGGAAATTGAAGGATATGTCAGTTTATGCCCCCACTCATGTTCCGGAAAGGATGTCAATGTTGAATCTGGATACAAACAGATGGTAGAGAAAAAGGGTCGTTCCACCCCACCATTGTGGTTTATTAGTGCTGAAGAGCTGGATTCCTTGTCATCATACATGAGAGGAAGGCTTACACTAGAGAAGGTCAATGCAGCTATTAATGACATGGTGGCAGGAGATCGATGGGAAAAAGCCTTGAAAAGTGAATTAAATCAGGAAATAAGAGATATAGCAATGCATGAAGCTGTAAAGGGAAAACACTTCTTTCTCGAAACTGACATGAAGGGACCATCCTTGAAGCCTGACAACACTGGAAAAGCAATACTAACTGTTCTTCGTCACCTTGATCGTGTAAGTGAGACACGGATTGGTCATCATCGGGTGATCATTCTTTTGAAGCCTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

276

Amino Acids

30.99

Weight (kDa)

8.26

Isoelectric Point (pI)

40.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 42 - 266 4.6e-53 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 234
AccII CGCG 2 cut(s) 190, 291
AccIII TCCGGA 1 cut(s) 425
AciI CCGC 1 cut(s) 17
AcoI YGGCCR 2 cut(s) 204, 228
AcuI CTGAAG 4 cut(s) 98, 245, 393, 534
AcyI GRCGYC 1 cut(s) 122
AfaI GTAC 2 cut(s) 200, 338
AflIII ACRYGT 1 cut(s) 195
AgsI TTSAA 7 cut(s) 41, 151, 392, 446, 622, 721, 820
AjnI CCWGG 1 cut(s) 267
AjuI GAANNNNNNNTTGG 2 cut(s) 13, 45
AleI CACNNNNGTG 1 cut(s) 496
AluBI AGCT 6 cut(s) 169, 259, 305, 520, 578, 668
AluI AGCT 6 cut(s) 169, 259, 305, 520, 578, 668
Alw21I GWGCWC 1 cut(s) 171
Alw26I GTCTC 2 cut(s) 166, 776
AlwNI CAGNNNCTG 1 cut(s) 356
Aor13HI TCCGGA 1 cut(s) 425
AoxI GGCC 4 cut(s) 204, 228, 249, 270
ApeKI GCWGC 3 cut(s) 210, 239, 575
AseI ATTAAT 1 cut(s) 582
AspLEI GCGC 1 cut(s) 190
AspS9I GGNCC 3 cut(s) 265, 280, 710
AsuC2I CCSGG 3 cut(s) 145, 203, 284
AsuHPI GGTGA 3 cut(s) 208, 755, 817
AvaII GGWCC 3 cut(s) 265, 280, 710
BanII GRGCYC 1 cut(s) 171
Bbv12I GWGCWC 1 cut(s) 171
BbvI GCAGC 3 cut(s) 222, 251, 587
BccI CCATC 7 cut(s) 94, 281, 327, 340, 457, 600, 721
BceAI ACGGC 3 cut(s) 215, 227, 243
BcgI CGANNNNNNTGC 2 cut(s) 105, 139
BciT130I CCWGG 1 cut(s) 269
BciVI GTATCC 1 cut(s) 446
BclI TGATCA 1 cut(s) 807
BcnI CCSGG 3 cut(s) 145, 203, 284
BcoDI GTCTC 2 cut(s) 166, 776
BfaI CTAG 2 cut(s) 302, 560
BfuI GTATCC 1 cut(s) 446
BglI GCCNNNNNGGC 1 cut(s) 248
BisI GCNGC 3 cut(s) 211, 240, 576
BlsI GCNGC 3 cut(s) 212, 241, 577
Bme1390I CCNGG 4 cut(s) 145, 203, 269, 284
Bme18I GGWCC 3 cut(s) 265, 280, 710
BmgT120I GGNCC 3 cut(s) 265, 280, 710
BmiI GGNNCC 1 cut(s) 711
BmrFI CCNGG 4 cut(s) 145, 203, 269, 284
BmsI GCATC 1 cut(s) 353
BoxI GACNNNNGTC 1 cut(s) 230
BpuMI CCSGG 3 cut(s) 145, 203, 284
Bsa29I ATCGAT 2 cut(s) 361, 604
BsaHI GRCGYC 1 cut(s) 122
BsaI GGTCTC 1 cut(s) 166
BsaJI CCNNGG 2 cut(s) 172, 252
BsaWI WCCGGW 1 cut(s) 425
Bse1I ACTGG 1 cut(s) 739
Bse3DI GCAATG 2 cut(s) 366, 663
BseAI TCCGGA 1 cut(s) 425
BseBI CCWGG 1 cut(s) 269
BseCI ATCGAT 2 cut(s) 361, 604
BseDI CCNNGG 2 cut(s) 172, 252
BseGI GGATG 2 cut(s) 439, 713
BseMI GCAATG 2 cut(s) 366, 663
BseNI ACTGG 1 cut(s) 739
BseX3I CGGCCG 1 cut(s) 228
BseXI GCAGC 3 cut(s) 222, 251, 587
Bsh1236I CGCG 2 cut(s) 190, 291
Bsh1285I CGRYCG 1 cut(s) 231
BshFI GGCC 4 cut(s) 206, 230, 251, 272
BshVI ATCGAT 2 cut(s) 361, 604
BsiEI CGRYCG 1 cut(s) 231
BsiHKAI GWGCWC 1 cut(s) 171
BsiSI CCGG 4 cut(s) 144, 203, 283, 426
BslFI GGGAC 1 cut(s) 723
BsmAI GTCTC 2 cut(s) 166, 776
BsmFI GGGAC 1 cut(s) 723
BsnI GGCC 4 cut(s) 206, 230, 251, 272
Bso31I GGTCTC 1 cut(s) 166
Bsp1286I GDGCHC 1 cut(s) 171
Bsp13I TCCGGA 1 cut(s) 425
Bsp143I GATC 6 cut(s) 64, 138, 358, 601, 769, 807
Bsp19I CCATGG 1 cut(s) 252
Bsp68I TCGCGA 1 cut(s) 291
BspACI CCGC 1 cut(s) 17
BspANI GGCC 4 cut(s) 206, 230, 251, 272
BspDI ATCGAT 2 cut(s) 361, 604
BspEI TCCGGA 1 cut(s) 425
BspFNI CGCG 2 cut(s) 190, 291
BspLI GGNNCC 1 cut(s) 711
BspQI GCTCTTC 1 cut(s) 510
BspTNI GGTCTC 1 cut(s) 166
BsrDI GCAATG 2 cut(s) 366, 663
BsrI ACTGG 1 cut(s) 739
BssECI CCNNGG 2 cut(s) 172, 252
BssMI GATC 6 cut(s) 64, 138, 358, 601, 769, 807
BssNI GRCGYC 1 cut(s) 122
BssT1I CCWWGG 2 cut(s) 172, 252
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 2 cut(s) 155, 754
Bst6I CTCTTC 2 cut(s) 74, 510
BstACI GRCGYC 1 cut(s) 122
BstC8I GCNNGC 1 cut(s) 208
BstDSI CCRYGG 1 cut(s) 252
BstF5I GGATG 2 cut(s) 439, 713
BstFNI CGCG 2 cut(s) 190, 291
BstHHI GCGC 1 cut(s) 190
BstKTI GATC 6 cut(s) 67, 141, 361, 604, 772, 810
BstMAI GTCTC 2 cut(s) 166, 776
BstMBI GATC 6 cut(s) 64, 138, 358, 601, 769, 807
BstMCI CGRYCG 1 cut(s) 231
BstMWI GCNNNNNNNGC 5 cut(s) 104, 113, 175, 236, 248
BstNI CCWGG 1 cut(s) 269
BstNSI RCATGY 1 cut(s) 199
BstPAI GACNNNNGTC 1 cut(s) 230
BstSCI CCNGG 4 cut(s) 143, 201, 267, 282
BstUI CGCG 2 cut(s) 190, 291
BstV1I GCAGC 3 cut(s) 222, 251, 587
BstXI CCANNNNNNTGG 1 cut(s) 498
BstZI CGGCCG 1 cut(s) 228
Bsu15I ATCGAT 2 cut(s) 361, 604
BsuI GTATCC 1 cut(s) 446
BsuRI GGCC 4 cut(s) 206, 230, 251, 272
BsuTUI ATCGAT 2 cut(s) 361, 604
BtgI CCRYGG 1 cut(s) 252
BtgZI GCGATG 1 cut(s) 113
BtsCI GGATG 2 cut(s) 439, 713
BtsIMutI CAGTG 2 cut(s) 732, 826
BtuMI TCGCGA 1 cut(s) 291
Cac8I GCNNGC 1 cut(s) 208
CaiI CAGNNNCTG 1 cut(s) 356
CfoI GCGC 1 cut(s) 190
Cfr13I GGNCC 3 cut(s) 265, 280, 710
ClaI ATCGAT 2 cut(s) 361, 604
CseI GACGC 3 cut(s) 111, 202, 245
Csp6I GTAC 2 cut(s) 199, 337
CviAII CATG 7 cut(s) 196, 253, 420, 541, 589, 662, 703
CviQI GTAC 2 cut(s) 199, 337
DpnI GATC 6 cut(s) 66, 140, 360, 603, 771, 809
DpnII GATC 6 cut(s) 64, 138, 358, 601, 769, 807
EaeI YGGCCR 2 cut(s) 204, 228
EagI CGGCCG 1 cut(s) 228
Eam1104I CTCTTC 2 cut(s) 74, 510
EarI CTCTTC 2 cut(s) 74, 510
Ecl136II GAGCTC 1 cut(s) 169
EclXI CGGCCG 1 cut(s) 228
Eco130I CCWWGG 2 cut(s) 172, 252
Eco24I GRGCYC 1 cut(s) 171
Eco31I GGTCTC 1 cut(s) 166
Eco47I GGWCC 3 cut(s) 265, 280, 710
Eco52I CGGCCG 1 cut(s) 228
Eco53kI GAGCTC 1 cut(s) 169
Eco57I CTGAAG 4 cut(s) 98, 245, 393, 534
EcoICRI GAGCTC 1 cut(s) 169
EcoRII CCWGG 1 cut(s) 267
EcoT14I CCWWGG 2 cut(s) 172, 252
EcoT22I ATGCAT 2 cut(s) 368, 663
EcoT38I GRGCYC 1 cut(s) 171
ErhI CCWWGG 2 cut(s) 172, 252
FaeI CATG 7 cut(s) 199, 256, 423, 544, 592, 665, 706
FaqI GGGAC 1 cut(s) 723
FatI CATG 7 cut(s) 195, 252, 419, 540, 588, 661, 702
FauI CCCGC 1 cut(s) 24
FbaI TGATCA 1 cut(s) 807
FblI GTMKAC 1 cut(s) 234
Fnu4HI GCNGC 3 cut(s) 211, 240, 576
FokI GGATG 2 cut(s) 446, 700
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 3 cut(s) 211, 240, 576
FspBI CTAG 2 cut(s) 302, 560
GlaI GCGC 1 cut(s) 189
GluI GCNGC 3 cut(s) 211, 240, 576
HaeIII GGCC 4 cut(s) 206, 230, 251, 272
HapII CCGG 4 cut(s) 144, 203, 283, 426
HgaI GACGC 3 cut(s) 111, 202, 245
HhaI GCGC 1 cut(s) 190
Hin1I GRCGYC 1 cut(s) 122
Hin1II CATG 7 cut(s) 199, 256, 423, 544, 592, 665, 706
Hin6I GCGC 1 cut(s) 188
HinP1I GCGC 1 cut(s) 188
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HinfI GANTC 4 cut(s) 57, 131, 446, 524
HpaII CCGG 4 cut(s) 144, 203, 283, 426
HphI GGTGA 3 cut(s) 208, 755, 817
Hpy166II GTNNAC 1 cut(s) 235
Hpy188I TCNGA 2 cut(s) 78, 166
Hpy188III TCNNGA 5 cut(s) 290, 426, 450, 638, 692
Hpy8I GTNNAC 1 cut(s) 235
Hpy99I CGWCG 4 cut(s) 127, 236, 239, 296
HpyAV CCTTC 5 cut(s) 241, 386, 543, 559, 700
HpyCH4III ACNGT 2 cut(s) 155, 754
HpyCH4V TGCA 3 cut(s) 366, 575, 661
HpyF10VI GCNNNNNNNGC 5 cut(s) 104, 113, 175, 236, 248
Hsp92I GRCGYC 1 cut(s) 122
Hsp92II CATG 7 cut(s) 199, 256, 423, 544, 592, 665, 706
HspAI GCGC 1 cut(s) 188
Kpn2I TCCGGA 1 cut(s) 425
Ksp22I TGATCA 1 cut(s) 807
Kzo9I GATC 6 cut(s) 64, 138, 358, 601, 769, 807
LguI GCTCTTC 1 cut(s) 510
LmnI GCTCC 3 cut(s) 166, 174, 256
Lsp1109I GCAGC 3 cut(s) 222, 251, 587
LweI GCATC 1 cut(s) 353
MaeI CTAG 2 cut(s) 302, 560
MaeIII GTNAC 2 cut(s) 214, 761
MalI GATC 6 cut(s) 66, 140, 360, 603, 771, 809
MboI GATC 6 cut(s) 64, 138, 358, 601, 769, 807
MboII GAAGA 4 cut(s) 74, 91, 527, 749
MhlI GDGCHC 1 cut(s) 171
MluCI AATT 2 cut(s) 387, 629
MlyI GAGTC 1 cut(s) 125
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 3 cut(s) 81, 97, 539
Mph1103I ATGCAT 2 cut(s) 368, 663
MroI TCCGGA 1 cut(s) 425
MseI TTAA 2 cut(s) 582, 632
MslI CAYNNNNRTG 1 cut(s) 496
MspI CCGG 4 cut(s) 144, 203, 283, 426
MspR9I CCNGG 4 cut(s) 145, 203, 269, 284
MvaI CCWGG 1 cut(s) 269
MvnI CGCG 2 cut(s) 190, 291
MwoI GCNNNNNNNGC 5 cut(s) 104, 113, 175, 236, 248
NciI CCSGG 3 cut(s) 145, 203, 284
NcoI CCATGG 1 cut(s) 252
NdeII GATC 6 cut(s) 64, 138, 358, 601, 769, 807
NlaIII CATG 7 cut(s) 199, 256, 423, 544, 592, 665, 706
NlaIV GGNNCC 1 cut(s) 711
NmuCI GTSAC 2 cut(s) 214, 761
NruI TCGCGA 1 cut(s) 291
NsiI ATGCAT 2 cut(s) 368, 663
NspI RCATGY 1 cut(s) 199
OliI CACNNNNGTG 1 cut(s) 496
PciI ACATGT 1 cut(s) 195
PciSI GCTCTTC 1 cut(s) 510
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PfeI GAWTC 3 cut(s) 57, 446, 524
PkrI GCNGC 3 cut(s) 212, 241, 577
PleI GAGTC 1 cut(s) 125
PpsI GAGTC 1 cut(s) 125
PscI ACATGT 1 cut(s) 195
PshAI GACNNNNGTC 1 cut(s) 230
PshBI ATTAAT 1 cut(s) 582
Psp124BI GAGCTC 1 cut(s) 171
Psp6I CCWGG 1 cut(s) 267
PspGI CCWGG 1 cut(s) 267
PspN4I GGNNCC 1 cut(s) 711
PspPI GGNCC 3 cut(s) 265, 280, 710
PstNI CAGNNNCTG 1 cut(s) 356
RruI TCGCGA 1 cut(s) 291
RsaI GTAC 2 cut(s) 200, 338
RsaNI GTAC 2 cut(s) 199, 337
RseI CAYNNNNRTG 1 cut(s) 496
SacI GAGCTC 1 cut(s) 171
SalI GTCGAC 1 cut(s) 233
SapI GCTCTTC 1 cut(s) 510
SaqAI TTAA 2 cut(s) 582, 632
SatI GCNGC 3 cut(s) 211, 240, 576
Sau3AI GATC 6 cut(s) 64, 138, 358, 601, 769, 807
Sau96I GGNCC 3 cut(s) 265, 280, 710
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 4 cut(s) 145, 203, 269, 284
SduI GDGCHC 1 cut(s) 171
SetI ASST 9 cut(s) 171, 261, 267, 307, 522, 570, 580, 670, 768
SfaNI GCATC 1 cut(s) 353
SgrDI CGTCGACG 1 cut(s) 233
SinI GGWCC 3 cut(s) 265, 280, 710
SmiMI CAYNNNNRTG 1 cut(s) 496
Sse9I AATT 2 cut(s) 387, 629
SsiI CCGC 1 cut(s) 17
SspMI CTAG 2 cut(s) 302, 560
SstI GAGCTC 1 cut(s) 171
StyD4I CCNGG 4 cut(s) 143, 201, 267, 282
StyI CCWWGG 2 cut(s) 172, 252
TaaI ACNGT 2 cut(s) 155, 754
TaqI TCGA 6 cut(s) 60, 67, 234, 361, 604, 693
TasI AATT 2 cut(s) 387, 629
TfiI GAWTC 3 cut(s) 57, 446, 524
Tru1I TTAA 2 cut(s) 582, 632
Tru9I TTAA 2 cut(s) 582, 632
TscAI CASTG 1 cut(s) 739
TseFI GTSAC 2 cut(s) 214, 761
TseI GCWGC 3 cut(s) 210, 239, 575
Tsp45I GTSAC 2 cut(s) 214, 761
TspDTI ATGAA 2 cut(s) 678, 719
TspGWI ACGGA 1 cut(s) 802
TspRI CASTG 1 cut(s) 739
VpaK11BI GGWCC 3 cut(s) 265, 280, 710
VspI ATTAAT 1 cut(s) 582
XceI RCATGY 1 cut(s) 199
XmiI GTMKAC 1 cut(s) 234
XspI CTAG 2 cut(s) 302, 560
Zsp2I ATGCAT 2 cut(s) 368, 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.