FvH4_7g11150

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001324
Physical Location & Seq
Forward (+)
10424019 .. 10427567
3549 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g11150.t2

Sequence Viewer

Length: 810 bp
ATGGAGGCGGAGAAAGCAGGGGCGTCGCTGGACTCGCTGGTCTCCGGGTTCAACAGCCGGATCTCGGAGCTCCAAGAGCTCGTTATTGCCCGAAACATGTACCCGGCCAGCAGCGTCACCGATCTGTCGGCCGTTGACGCCGCCGTGAAGGCCATGGAGGTTCAGGTCCAGGCCATCAAGGATCGCGTTCGCGACGAAACCCTAGCCATCCCCAAAGCTAAAAAACTGATCGATGCATCGTTGAAGCAGCAAAGGATATTGAAGGACATGTCAATTTATGCGCCAGCTCATGTTCCTGAAAGAATGTCAATGTTGAATTTGGATACAAATAGATGTTTATTTCCAGAAAGCTCTCAGCAAAACGCAGGTTCTGGGATCTTCAAGCTTGAGGAGGATCCTGCACCACCACCTAAGGAGAAAAAGGGTCGTCTTCCACCACCATTGTGGTTTTTAAGTGCTGAAGAGCTGGATTCCTTGTCATCATACATGAGAGGAAGGCTTACACTTGAGAAAGTCAATGCAGCTATTAATGACATGGCAACATACGCTGAAGCAAATTATCAGCTCATAGCTGCCCCAAAGAAGAATGTGGCAGGAGATCGTTGGGAGAAGGCCTTGGAAATAAGAGATATTGCAATGCATGAAGCCGTGAAGGGAAAACATTTCTTTCTCGAAACTGACATGAAGGGACCATCCTTGAAGCTTGACAACACTGGAAAAGCAATACTAACTGTTCTTCGTCACCTGGGTCGTGTAAGTGAGACACGAATTGGTCATCATCGGGTGATCATCCTTTTGAAGCCTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

270

Amino Acids

29.88

Weight (kDa)

8.81

Isoelectric Point (pI)

43.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 9 - 245 1.2e-74 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 38
Acc36I ACCTGC 1 cut(s) 356
AccII CGCG 2 cut(s) 186, 192
AciI CCGC 2 cut(s) 8, 141
AclWI GGATC 5 cut(s) 68, 189, 383, 389, 402
AcoI YGGCCR 2 cut(s) 105, 129
AcsI RAATTY 1 cut(s) 316
AcuI CTGAAG 2 cut(s) 480, 570
AcyI GRCGYC 2 cut(s) 23, 138
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 1 cut(s) 64
AflIII ACRYGT 2 cut(s) 96, 267
AgsI TTSAA 7 cut(s) 52, 244, 262, 316, 382, 700, 799
AjnI CCWGG 2 cut(s) 168, 744
AleI CACNNNNGTG 1 cut(s) 442
Alw21I GWGCWC 2 cut(s) 72, 81
Alw26I GTCTC 2 cut(s) 46, 755
AlwI GGATC 5 cut(s) 68, 189, 383, 389, 402
AlwNI CAGNNNCTG 1 cut(s) 371
AoxI GGCC 5 cut(s) 105, 129, 150, 171, 612
ApeKI GCWGC 4 cut(s) 111, 247, 521, 572
ApoI RAATTY 1 cut(s) 316
AseI ATTAAT 1 cut(s) 528
AspLEI GCGC 1 cut(s) 283
AspS9I GGNCC 2 cut(s) 166, 689
AsuC2I CCSGG 2 cut(s) 46, 104
AsuHPI GGTGA 3 cut(s) 109, 734, 796
AvaII GGWCC 2 cut(s) 166, 689
AxyI CCTNAGG 1 cut(s) 411
BamHI GGATCC 1 cut(s) 394
BanII GRGCYC 2 cut(s) 72, 81
BbsI GAAGAC 1 cut(s) 422
Bbv12I GWGCWC 2 cut(s) 72, 81
BbvI GCAGC 4 cut(s) 123, 259, 533, 559
BccI CCATC 3 cut(s) 182, 215, 700
BceAI ACGGC 3 cut(s) 116, 128, 632
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BciT130I CCWGG 2 cut(s) 170, 746
BciVI GTATCC 1 cut(s) 316
BclI TGATCA 1 cut(s) 786
BcnI CCSGG 2 cut(s) 46, 104
BcoDI GTCTC 2 cut(s) 46, 755
BfaI CTAG 1 cut(s) 203
BfuAI ACCTGC 1 cut(s) 356
BfuI GTATCC 1 cut(s) 316
BglI GCCNNNNNGGC 1 cut(s) 149
BisI GCNGC 5 cut(s) 112, 141, 248, 522, 573
BlsI GCNGC 5 cut(s) 113, 142, 249, 523, 574
Bme1390I CCNGG 4 cut(s) 46, 104, 170, 746
Bme18I GGWCC 2 cut(s) 166, 689
BmgT120I GGNCC 2 cut(s) 166, 689
BmiI GGNNCC 2 cut(s) 396, 690
BmrFI CCNGG 4 cut(s) 46, 104, 170, 746
BmsI GCATC 2 cut(s) 223, 245
BpiI GAAGAC 1 cut(s) 422
BpuEI CTTGAG 2 cut(s) 407, 527
BpuMI CCSGG 2 cut(s) 46, 104
Bsa29I ATCGAT 1 cut(s) 231
BsaHI GRCGYC 2 cut(s) 23, 138
BsaI GGTCTC 1 cut(s) 46
BsaJI CCNNGG 3 cut(s) 153, 615, 745
Bsc4I CCNNNNNNNGG 1 cut(s) 64
Bse1I ACTGG 1 cut(s) 718
Bse21I CCTNAGG 1 cut(s) 411
Bse3DI GCAATG 1 cut(s) 642
BseBI CCWGG 2 cut(s) 170, 746
BseCI ATCGAT 1 cut(s) 231
BseDI CCNNGG 3 cut(s) 153, 615, 745
BseGI GGATG 3 cut(s) 207, 692, 789
BseLI CCNNNNNNNGG 1 cut(s) 64
BseMI GCAATG 1 cut(s) 642
BseMII CTCAG 1 cut(s) 368
BseNI ACTGG 1 cut(s) 718
BseRI GAGGAG 1 cut(s) 404
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 4 cut(s) 123, 259, 533, 559
BsgI GTGCAG 1 cut(s) 384
Bsh1236I CGCG 2 cut(s) 186, 192
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 5 cut(s) 107, 131, 152, 173, 614
BshVI ATCGAT 1 cut(s) 231
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 2 cut(s) 72, 81
BsiSI CCGG 3 cut(s) 45, 58, 104
BslFI GGGAC 1 cut(s) 702
BslI CCNNNNNNNGG 1 cut(s) 64
BsmAI GTCTC 2 cut(s) 46, 755
BsmFI GGGAC 1 cut(s) 702
BsnI GGCC 5 cut(s) 107, 131, 152, 173, 614
Bso31I GGTCTC 1 cut(s) 46
Bsp1286I GDGCHC 2 cut(s) 72, 81
Bsp143I GATC 8 cut(s) 60, 121, 181, 228, 375, 394, 598, 786
Bsp19I CCATGG 1 cut(s) 153
Bsp68I TCGCGA 1 cut(s) 192
BspACI CCGC 2 cut(s) 8, 141
BspANI GGCC 5 cut(s) 107, 131, 152, 173, 614
BspCNI CTCAG 1 cut(s) 367
BspDI ATCGAT 1 cut(s) 231
BspFNI CGCG 2 cut(s) 186, 192
BspLI GGNNCC 2 cut(s) 396, 690
BspMI ACCTGC 1 cut(s) 356
BspPI GGATC 5 cut(s) 68, 189, 383, 389, 402
BspQI GCTCTTC 1 cut(s) 456
BspTNI GGTCTC 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 642
BsrI ACTGG 1 cut(s) 718
BssECI CCNNGG 3 cut(s) 153, 615, 745
BssMI GATC 8 cut(s) 60, 121, 181, 228, 375, 394, 598, 786
BssNI GRCGYC 2 cut(s) 23, 138
BssT1I CCWWGG 2 cut(s) 153, 615
Bst2UI CCWGG 2 cut(s) 170, 746
Bst4CI ACNGT 1 cut(s) 733
Bst6I CTCTTC 1 cut(s) 456
BstACI GRCGYC 2 cut(s) 23, 138
BstC8I GCNNGC 2 cut(s) 109, 285
BstDEI CTNAG 2 cut(s) 354, 411
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 3 cut(s) 207, 692, 789
BstFNI CGCG 2 cut(s) 186, 192
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 8 cut(s) 63, 124, 184, 231, 378, 397, 601, 789
BstMAI GTCTC 2 cut(s) 46, 755
BstMBI GATC 8 cut(s) 60, 121, 181, 228, 375, 394, 598, 786
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 6 cut(s) 14, 34, 76, 137, 149, 545
BstNI CCWGG 2 cut(s) 170, 746
BstNSI RCATGY 2 cut(s) 100, 271
BstSCI CCNGG 4 cut(s) 44, 102, 168, 744
BstUI CGCG 2 cut(s) 186, 192
BstV1I GCAGC 4 cut(s) 123, 259, 533, 559
BstV2I GAAGAC 1 cut(s) 422
BstX2I RGATCY 3 cut(s) 60, 375, 394
BstXI CCANNNNNNTGG 1 cut(s) 444
BstYI RGATCY 3 cut(s) 60, 375, 394
BstZI CGGCCG 1 cut(s) 129
Bsu15I ATCGAT 1 cut(s) 231
Bsu36I CCTNAGG 1 cut(s) 411
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 5 cut(s) 107, 131, 152, 173, 614
BsuTUI ATCGAT 1 cut(s) 231
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 3 cut(s) 207, 692, 789
BtsIMutI CAGTG 1 cut(s) 711
BtuMI TCGCGA 1 cut(s) 192
BveI ACCTGC 1 cut(s) 356
Cac8I GCNNGC 2 cut(s) 109, 285
CaiI CAGNNNCTG 1 cut(s) 371
CfoI GCGC 1 cut(s) 283
Cfr13I GGNCC 2 cut(s) 166, 689
ClaI ATCGAT 1 cut(s) 231
CseI GACGC 3 cut(s) 12, 103, 146
Csp6I GTAC 1 cut(s) 100
CspCI CAANNNNNGTGG 2 cut(s) 423, 458
CviAII CATG 8 cut(s) 97, 154, 268, 290, 487, 535, 641, 682
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 2 cut(s) 354, 411
DpnI GATC 8 cut(s) 62, 123, 183, 230, 377, 396, 600, 788
DpnII GATC 8 cut(s) 60, 121, 181, 228, 375, 394, 598, 786
DrdI GACNNNNNNGTC 1 cut(s) 38
DseDI GACNNNNNNGTC 1 cut(s) 38
EaeI YGGCCR 2 cut(s) 105, 129
EagI CGGCCG 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 2 cut(s) 70, 79
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 2 cut(s) 153, 615
Eco147I AGGCCT 1 cut(s) 614
Eco24I GRGCYC 2 cut(s) 72, 81
Eco31I GGTCTC 1 cut(s) 46
Eco47I GGWCC 2 cut(s) 166, 689
Eco52I CGGCCG 1 cut(s) 129
Eco53kI GAGCTC 2 cut(s) 70, 79
Eco57I CTGAAG 2 cut(s) 480, 570
Eco81I CCTNAGG 1 cut(s) 411
EcoICRI GAGCTC 2 cut(s) 70, 79
EcoRII CCWGG 2 cut(s) 168, 744
EcoT14I CCWWGG 2 cut(s) 153, 615
EcoT22I ATGCAT 2 cut(s) 238, 642
EcoT38I GRGCYC 2 cut(s) 72, 81
ErhI CCWWGG 2 cut(s) 153, 615
FaeI CATG 8 cut(s) 100, 157, 271, 293, 490, 538, 644, 685
FaqI GGGAC 1 cut(s) 702
FatI CATG 8 cut(s) 96, 153, 267, 289, 486, 534, 640, 681
FbaI TGATCA 1 cut(s) 786
Fnu4HI GCNGC 5 cut(s) 112, 141, 248, 522, 573
FokI GGATG 3 cut(s) 194, 679, 776
FriOI GRGCYC 2 cut(s) 72, 81
Fsp4HI GCNGC 5 cut(s) 112, 141, 248, 522, 573
FspBI CTAG 1 cut(s) 203
GlaI GCGC 1 cut(s) 282
GluI GCNGC 5 cut(s) 112, 141, 248, 522, 573
HaeIII GGCC 5 cut(s) 107, 131, 152, 173, 614
HapII CCGG 3 cut(s) 45, 58, 104
HgaI GACGC 3 cut(s) 12, 103, 146
HhaI GCGC 1 cut(s) 283
Hin1I GRCGYC 2 cut(s) 23, 138
Hin1II CATG 8 cut(s) 100, 157, 271, 293, 490, 538, 644, 685
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HindIII AAGCTT 2 cut(s) 383, 701
HinfI GANTC 2 cut(s) 32, 470
HpaII CCGG 3 cut(s) 45, 58, 104
HphI GGTGA 3 cut(s) 109, 734, 796
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 1 cut(s) 67
Hpy188III TCNNGA 4 cut(s) 191, 296, 344, 671
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 2 cut(s) 28, 197
HpyAV CCTTC 6 cut(s) 142, 256, 489, 604, 646, 679
HpyCH4III ACNGT 1 cut(s) 733
HpyCH4V TGCA 5 cut(s) 236, 401, 521, 635, 640
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 34, 76, 137, 149, 545
HpyF3I CTNAG 2 cut(s) 354, 411
Hsp92I GRCGYC 2 cut(s) 23, 138
Hsp92II CATG 8 cut(s) 100, 157, 271, 293, 490, 538, 644, 685
HspAI GCGC 1 cut(s) 281
Ksp22I TGATCA 1 cut(s) 786
Kzo9I GATC 8 cut(s) 60, 121, 181, 228, 375, 394, 598, 786
LguI GCTCTTC 1 cut(s) 456
LmnI GCTCC 2 cut(s) 67, 75
Lsp1109I GCAGC 4 cut(s) 123, 259, 533, 559
LweI GCATC 2 cut(s) 223, 245
MaeI CTAG 1 cut(s) 203
MaeIII GTNAC 2 cut(s) 115, 740
MalI GATC 8 cut(s) 62, 123, 183, 230, 377, 396, 600, 788
MboI GATC 8 cut(s) 60, 121, 181, 228, 375, 394, 598, 786
MboII GAAGA 5 cut(s) 370, 422, 473, 595, 728
MflI RGATCY 3 cut(s) 60, 375, 394
MhlI GDGCHC 2 cut(s) 72, 81
MluCI AATT 4 cut(s) 273, 316, 556, 768
MlyI GAGTC 1 cut(s) 26
MnlI CCTC 4 cut(s) 151, 382, 385, 485
Mph1103I ATGCAT 2 cut(s) 238, 642
MseI TTAA 2 cut(s) 452, 528
MslI CAYNNNNRTG 1 cut(s) 442
MspI CCGG 3 cut(s) 45, 58, 104
MspR9I CCNGG 4 cut(s) 46, 104, 170, 746
MvaI CCWGG 2 cut(s) 170, 746
MvnI CGCG 2 cut(s) 186, 192
MwoI GCNNNNNNNGC 6 cut(s) 14, 34, 76, 137, 149, 545
NciI CCSGG 2 cut(s) 46, 104
NcoI CCATGG 1 cut(s) 153
NdeII GATC 8 cut(s) 60, 121, 181, 228, 375, 394, 598, 786
NlaIII CATG 8 cut(s) 100, 157, 271, 293, 490, 538, 644, 685
NlaIV GGNNCC 2 cut(s) 396, 690
NmuCI GTSAC 2 cut(s) 115, 740
NruI TCGCGA 1 cut(s) 192
NsiI ATGCAT 2 cut(s) 238, 642
NspI RCATGY 2 cut(s) 100, 271
OliI CACNNNNGTG 1 cut(s) 442
PceI AGGCCT 1 cut(s) 614
PciI ACATGT 2 cut(s) 96, 267
PciSI GCTCTTC 1 cut(s) 456
PfeI GAWTC 1 cut(s) 470
PkrI GCNGC 5 cut(s) 113, 142, 249, 523, 574
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PscI ACATGT 2 cut(s) 96, 267
PshBI ATTAAT 1 cut(s) 528
Psp124BI GAGCTC 2 cut(s) 72, 81
Psp6I CCWGG 2 cut(s) 168, 744
PspGI CCWGG 2 cut(s) 168, 744
PspN4I GGNNCC 2 cut(s) 396, 690
PspPI GGNCC 2 cut(s) 166, 689
PstNI CAGNNNCTG 1 cut(s) 371
PsuI RGATCY 3 cut(s) 60, 375, 394
RruI TCGCGA 1 cut(s) 192
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
RseI CAYNNNNRTG 1 cut(s) 442
SacI GAGCTC 2 cut(s) 72, 81
SapI GCTCTTC 1 cut(s) 456
SaqAI TTAA 2 cut(s) 452, 528
SatI GCNGC 5 cut(s) 112, 141, 248, 522, 573
Sau3AI GATC 8 cut(s) 60, 121, 181, 228, 375, 394, 598, 786
Sau96I GGNCC 2 cut(s) 166, 689
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 4 cut(s) 46, 104, 170, 746
SduI GDGCHC 2 cut(s) 72, 81
SfaNI GCATC 2 cut(s) 223, 245
SinI GGWCC 2 cut(s) 166, 689
SmiMI CAYNNNNRTG 1 cut(s) 442
SmlI CTYRAG 2 cut(s) 386, 506
SmoI CTYRAG 2 cut(s) 386, 506
Sse9I AATT 4 cut(s) 273, 316, 556, 768
SseBI AGGCCT 1 cut(s) 614
SsiI CCGC 2 cut(s) 8, 141
SspMI CTAG 1 cut(s) 203
SstI GAGCTC 2 cut(s) 72, 81
StuI AGGCCT 1 cut(s) 614
StyD4I CCNGG 4 cut(s) 44, 102, 168, 744
StyI CCWWGG 2 cut(s) 153, 615
TaaI ACNGT 1 cut(s) 733
TaqI TCGA 2 cut(s) 231, 672
TasI AATT 4 cut(s) 273, 316, 556, 768
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 1 cut(s) 470
Tru1I TTAA 2 cut(s) 452, 528
Tru9I TTAA 2 cut(s) 452, 528
TscAI CASTG 1 cut(s) 718
TseFI GTSAC 2 cut(s) 115, 740
TseI GCWGC 4 cut(s) 111, 247, 521, 572
Tsp45I GTSAC 2 cut(s) 115, 740
TspDTI ATGAA 2 cut(s) 657, 698
TspRI CASTG 1 cut(s) 718
VpaK11BI GGWCC 2 cut(s) 166, 689
VspI ATTAAT 1 cut(s) 528
XapI RAATTY 1 cut(s) 316
XceI RCATGY 2 cut(s) 100, 271
XcmI CCANNNNNNNNNTGG 1 cut(s) 441
XspI CTAG 1 cut(s) 203
Zsp2I ATGCAT 2 cut(s) 238, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.