MD02G1209900.v1.1

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001324
Physical Location & Seq
Reverse (-)
21697190 .. 21701720
4531 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1209900.v1.1.491

Sequence Viewer

Length: 810 bp
ATGGAGGTAAAGACAGCAGGGGCGTCGTTGGATTCGGTTATCTCAGGGTTCAACCGTCGGATCGCGGAGCTTCAAGATCTCGTCATTGCGCGAAACATGTACCCGGCGAGCAGCGTCACCGATTTGTCGGCCCTCGACGCGGCCGTGAAGGCCATGGAGCTTCAGGTTCAGGCCATCAAGGACCGCTTGCGTGACGAAACCCTAGCCATCCCTAAAGCTAAAAAACTCATCGATGCCGCGCTGAAGCAGCAAAAGAAATTGCAGGACATGTCAGTTTATGTGCCCTCGCATGTTCCCGAAAGAATGTCGATGTTGAATTTGGATACTAATAAGTGTTTGTTTCCAGAAGGATCTCAACAAAATACCGGTTTTGGGTCCTTCAAGCTTGAGGAAGAGCCTGCTGCACTACCTAAGGAGAAAAAGGGACGTTCCCCTCCACCATTGTGGTTTATAACTGCTGAAGAGCTGGATTCTTTGTCAGGATACATGAGGGGAAGGCTTACAGTAGAGAAGATCAATGCAGCTATTAATGACATGGCGACGTATGCAGAAGCAAACGCTCAGCTGATATTTGCCCCAAAGAAGAAGGTAGCAGGAGATCTTTGGGAAAAGGCGCTGGAAATGAGGGACATTGCAATGACAGAAGGAATAAAGGGAAAACACTTCTTTCTCGAAACTGACATGAAAGGACCAACCTTGAAGCCTGACAACACTGGAAAAGCAATACTAACTGTCCTTCGTCACCTTGGTCGTGTAAGCGAGACACGTATCGGGCCTCATCGAGTGATCATTCTATTAAAGCGTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

270

Amino Acids

29.84

Weight (kDa)

9.25

Isoelectric Point (pI)

44.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 9 - 259 5.3e-84 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 452
AccII CGCG 4 cut(s) 65, 91, 140, 239
AciI CCGC 4 cut(s) 65, 140, 184, 237
AclWI GGATC 2 cut(s) 68, 358
AcoI YGGCCR 1 cut(s) 141
AcsI RAATTY 1 cut(s) 316
AcuI CTGAAG 3 cut(s) 146, 263, 480
AcyI GRCGYC 1 cut(s) 23
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 2 cut(s) 139, 372
AflIII ACRYGT 3 cut(s) 96, 267, 764
AgeI ACCGGT 1 cut(s) 365
AgsI TTSAA 5 cut(s) 52, 74, 316, 382, 700
AleI CACNNNNGTG 1 cut(s) 442
AluBI AGCT 7 cut(s) 70, 160, 218, 385, 466, 524, 565
AluI AGCT 7 cut(s) 70, 160, 218, 385, 466, 524, 565
Alw26I GTCTC 1 cut(s) 755
AlwI GGATC 2 cut(s) 68, 358
AoxI GGCC 5 cut(s) 129, 141, 150, 171, 773
ApeKI GCWGC 4 cut(s) 111, 247, 401, 521
ApoI RAATTY 1 cut(s) 316
AseI ATTAAT 1 cut(s) 528
AsiGI ACCGGT 1 cut(s) 365
AspLEI GCGC 3 cut(s) 91, 241, 616
AspS9I GGNCC 5 cut(s) 130, 181, 375, 689, 773
AsuC2I CCSGG 1 cut(s) 104
AsuHPI GGTGA 2 cut(s) 109, 734
AvaII GGWCC 3 cut(s) 181, 375, 689
AxyI CCTNAGG 1 cut(s) 411
BaeGI GKGCMC 1 cut(s) 285
BbvI GCAGC 4 cut(s) 123, 259, 388, 533
BccI CCATC 2 cut(s) 182, 215
BceAI ACGGC 1 cut(s) 128
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BciVI GTATCC 2 cut(s) 316, 476
BclI TGATCA 1 cut(s) 786
BcnI CCSGG 1 cut(s) 104
BcoDI GTCTC 1 cut(s) 755
BfaI CTAG 1 cut(s) 203
BfoI RGCGCY 1 cut(s) 617
BfuI GTATCC 2 cut(s) 316, 476
BglI GCCNNNNNGGC 1 cut(s) 149
BglII AGATCT 2 cut(s) 76, 598
BisI GCNGC 6 cut(s) 112, 141, 237, 248, 402, 522
BlpI GCTNAGC 1 cut(s) 561
BlsI GCNGC 6 cut(s) 113, 142, 238, 249, 403, 523
Bme1390I CCNGG 1 cut(s) 104
Bme18I GGWCC 3 cut(s) 181, 375, 689
BmgT120I GGNCC 5 cut(s) 130, 181, 375, 689, 773
BmiI GGNNCC 1 cut(s) 376
BmrFI CCNGG 1 cut(s) 104
BmsI GCATC 1 cut(s) 223
Bpu1102I GCTNAGC 1 cut(s) 561
BpuEI CTTGAG 1 cut(s) 407
BpuMI CCSGG 1 cut(s) 104
Bsa29I ATCGAT 1 cut(s) 231
BsaAI YACGTR 1 cut(s) 767
BsaHI GRCGYC 1 cut(s) 23
BsaJI CCNNGG 2 cut(s) 153, 745
BsaWI WCCGGW 1 cut(s) 365
Bsc4I CCNNNNNNNGG 2 cut(s) 139, 372
Bse118I RCCGGY 1 cut(s) 365
Bse1I ACTGG 1 cut(s) 718
Bse21I CCTNAGG 1 cut(s) 411
Bse3DI GCAATG 3 cut(s) 84, 630, 642
BseCI ATCGAT 1 cut(s) 231
BseDI CCNNGG 2 cut(s) 153, 745
BseGI GGATG 1 cut(s) 207
BseLI CCNNNNNNNGG 2 cut(s) 139, 372
BseMI GCAATG 3 cut(s) 84, 630, 642
BseMII CTCAG 2 cut(s) 57, 575
BseNI ACTGG 1 cut(s) 718
BseSI GKGCMC 1 cut(s) 285
BseX3I CGGCCG 1 cut(s) 141
BseXI GCAGC 4 cut(s) 123, 259, 388, 533
BsgI GTGCAG 1 cut(s) 387
Bsh1236I CGCG 4 cut(s) 65, 91, 140, 239
Bsh1285I CGRYCG 1 cut(s) 144
BshFI GGCC 5 cut(s) 131, 143, 152, 173, 775
BshTI ACCGGT 1 cut(s) 365
BshVI ATCGAT 1 cut(s) 231
BsiEI CGRYCG 1 cut(s) 144
BsiSI CCGG 2 cut(s) 104, 366
BslFI GGGAC 2 cut(s) 438, 641
BslI CCNNNNNNNGG 2 cut(s) 139, 372
BsmAI GTCTC 1 cut(s) 755
BsmFI GGGAC 2 cut(s) 438, 641
BsnI GGCC 5 cut(s) 131, 143, 152, 173, 775
Bsp1286I GDGCHC 1 cut(s) 285
Bsp143I GATC 6 cut(s) 60, 76, 350, 513, 598, 786
Bsp1720I GCTNAGC 1 cut(s) 561
Bsp19I CCATGG 1 cut(s) 153
BspACI CCGC 4 cut(s) 65, 140, 184, 237
BspANI GGCC 5 cut(s) 131, 143, 152, 173, 775
BspCNI CTCAG 2 cut(s) 56, 574
BspDI ATCGAT 1 cut(s) 231
BspFNI CGCG 4 cut(s) 65, 91, 140, 239
BspLI GGNNCC 1 cut(s) 376
BspPI GGATC 2 cut(s) 68, 358
BspQI GCTCTTC 2 cut(s) 387, 456
BsrDI GCAATG 3 cut(s) 84, 630, 642
BsrFI RCCGGY 1 cut(s) 365
BsrI ACTGG 1 cut(s) 718
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 2 cut(s) 153, 745
BssMI GATC 6 cut(s) 60, 76, 350, 513, 598, 786
BssNI GRCGYC 1 cut(s) 23
BssT1I CCWWGG 2 cut(s) 153, 745
Bst4CI ACNGT 3 cut(s) 56, 505, 733
Bst6I CTCTTC 2 cut(s) 387, 456
BstACI GRCGYC 1 cut(s) 23
BstBAI YACGTR 1 cut(s) 767
BstC8I GCNNGC 3 cut(s) 109, 188, 399
BstDEI CTNAG 3 cut(s) 43, 411, 561
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 1 cut(s) 207
BstFNI CGCG 4 cut(s) 65, 91, 140, 239
BstH2I RGCGCY 1 cut(s) 617
BstHHI GCGC 3 cut(s) 91, 241, 616
BstKTI GATC 6 cut(s) 63, 79, 353, 516, 601, 789
BstMAI GTCTC 1 cut(s) 755
BstMBI GATC 6 cut(s) 60, 76, 350, 513, 598, 786
BstMCI CGRYCG 1 cut(s) 144
BstMWI GCNNNNNNNGC 4 cut(s) 137, 149, 247, 545
BstNSI RCATGY 3 cut(s) 100, 271, 293
BstSCI CCNGG 1 cut(s) 102
BstSLI GKGCMC 1 cut(s) 285
BstUI CGCG 4 cut(s) 65, 91, 140, 239
BstV1I GCAGC 4 cut(s) 123, 259, 388, 533
BstX2I RGATCY 3 cut(s) 76, 350, 598
BstXI CCANNNNNNTGG 1 cut(s) 444
BstYI RGATCY 3 cut(s) 76, 350, 598
BstZI CGGCCG 1 cut(s) 141
Bsu15I ATCGAT 1 cut(s) 231
Bsu36I CCTNAGG 1 cut(s) 411
BsuI GTATCC 2 cut(s) 316, 476
BsuRI GGCC 5 cut(s) 131, 143, 152, 173, 775
BsuTUI ATCGAT 1 cut(s) 231
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 1 cut(s) 207
BtsIMutI CAGTG 1 cut(s) 711
Cac8I GCNNGC 3 cut(s) 109, 188, 399
CfoI GCGC 3 cut(s) 91, 241, 616
Cfr10I RCCGGY 1 cut(s) 365
Cfr13I GGNCC 5 cut(s) 130, 181, 375, 689, 773
ClaI ATCGAT 1 cut(s) 231
CseI GACGC 4 cut(s) 12, 103, 146, 791
Csp6I GTAC 1 cut(s) 100
CspAI ACCGGT 1 cut(s) 365
CviAII CATG 7 cut(s) 97, 154, 268, 290, 487, 535, 682
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 3 cut(s) 43, 411, 561
DpnI GATC 6 cut(s) 62, 78, 352, 515, 600, 788
DpnII GATC 6 cut(s) 60, 76, 350, 513, 598, 786
EaeI YGGCCR 1 cut(s) 141
EagI CGGCCG 1 cut(s) 141
Eam1104I CTCTTC 2 cut(s) 387, 456
EarI CTCTTC 2 cut(s) 387, 456
EclXI CGGCCG 1 cut(s) 141
Eco130I CCWWGG 2 cut(s) 153, 745
Eco47I GGWCC 3 cut(s) 181, 375, 689
Eco52I CGGCCG 1 cut(s) 141
Eco57I CTGAAG 3 cut(s) 146, 263, 480
Eco81I CCTNAGG 1 cut(s) 411
EcoO109I RGGNCCY 1 cut(s) 375
EcoT14I CCWWGG 2 cut(s) 153, 745
ErhI CCWWGG 2 cut(s) 153, 745
FaeI CATG 7 cut(s) 100, 157, 271, 293, 490, 538, 685
FalI AAGNNNNNCTT 2 cut(s) 170, 202
FaqI GGGAC 2 cut(s) 438, 641
FatI CATG 7 cut(s) 96, 153, 267, 289, 486, 534, 681
FbaI TGATCA 1 cut(s) 786
Fnu4HI GCNGC 6 cut(s) 112, 141, 237, 248, 402, 522
FokI GGATG 1 cut(s) 194
Fsp4HI GCNGC 6 cut(s) 112, 141, 237, 248, 402, 522
FspBI CTAG 1 cut(s) 203
GlaI GCGC 3 cut(s) 90, 240, 615
GluI GCNGC 6 cut(s) 112, 141, 237, 248, 402, 522
HaeII RGCGCY 1 cut(s) 617
HaeIII GGCC 5 cut(s) 131, 143, 152, 173, 775
HapII CCGG 2 cut(s) 104, 366
HgaI GACGC 4 cut(s) 12, 103, 146, 791
HhaI GCGC 3 cut(s) 91, 241, 616
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 7 cut(s) 100, 157, 271, 293, 490, 538, 685
Hin6I GCGC 3 cut(s) 89, 239, 614
HinP1I GCGC 3 cut(s) 89, 239, 614
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 2 cut(s) 32, 470
HpaII CCGG 2 cut(s) 104, 366
HphI GGTGA 2 cut(s) 109, 734
Hpy188I TCNGA 1 cut(s) 60
Hpy188III TCNNGA 5 cut(s) 74, 296, 344, 480, 671
Hpy99I CGWCG 4 cut(s) 28, 60, 140, 544
HpyAV CCTTC 7 cut(s) 142, 341, 388, 489, 580, 638, 746
HpyCH4III ACNGT 3 cut(s) 56, 505, 733
HpyCH4IV ACGT 3 cut(s) 427, 542, 766
HpyCH4V TGCA 5 cut(s) 262, 404, 521, 548, 635
HpyF10VI GCNNNNNNNGC 4 cut(s) 137, 149, 247, 545
HpyF3I CTNAG 3 cut(s) 43, 411, 561
HpySE526I ACGT 3 cut(s) 427, 542, 766
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 7 cut(s) 100, 157, 271, 293, 490, 538, 685
HspAI GCGC 3 cut(s) 89, 239, 614
Ksp22I TGATCA 1 cut(s) 786
Kzo9I GATC 6 cut(s) 60, 76, 350, 513, 598, 786
LguI GCTCTTC 2 cut(s) 387, 456
LmnI GCTCC 2 cut(s) 67, 157
Lsp1109I GCAGC 4 cut(s) 123, 259, 388, 533
LweI GCATC 1 cut(s) 223
MaeI CTAG 1 cut(s) 203
MaeII ACGT 3 cut(s) 427, 542, 766
MaeIII GTNAC 3 cut(s) 115, 191, 740
MalI GATC 6 cut(s) 62, 78, 352, 515, 600, 788
MboI GATC 6 cut(s) 60, 76, 350, 513, 598, 786
MboII GAAGA 4 cut(s) 404, 473, 523, 595
MflI RGATCY 3 cut(s) 76, 350, 598
MhlI GDGCHC 1 cut(s) 285
MluCI AATT 2 cut(s) 257, 316
MmeI TCCRAC 2 cut(s) 9, 38
MnlI CCTC 7 cut(s) 143, 295, 382, 444, 483, 618, 786
MseI TTAA 2 cut(s) 528, 797
MslI CAYNNNNRTG 2 cut(s) 442, 635
MspA1I CMGCKG 1 cut(s) 565
MspI CCGG 2 cut(s) 104, 366
MspR9I CCNGG 1 cut(s) 104
MvnI CGCG 4 cut(s) 65, 91, 140, 239
MwoI GCNNNNNNNGC 4 cut(s) 137, 149, 247, 545
NciI CCSGG 1 cut(s) 104
NcoI CCATGG 1 cut(s) 153
NdeII GATC 6 cut(s) 60, 76, 350, 513, 598, 786
NlaIII CATG 7 cut(s) 100, 157, 271, 293, 490, 538, 685
NlaIV GGNNCC 1 cut(s) 376
NmuCI GTSAC 3 cut(s) 115, 191, 740
NspI RCATGY 3 cut(s) 100, 271, 293
OliI CACNNNNGTG 1 cut(s) 442
PciI ACATGT 2 cut(s) 96, 267
PciSI GCTCTTC 2 cut(s) 387, 456
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PfeI GAWTC 2 cut(s) 32, 470
PinAI ACCGGT 1 cut(s) 365
PkrI GCNGC 6 cut(s) 113, 142, 238, 249, 403, 523
Ppu21I YACGTR 1 cut(s) 767
PpuMI RGGWCCY 1 cut(s) 375
PscI ACATGT 2 cut(s) 96, 267
PshBI ATTAAT 1 cut(s) 528
PsiI TTATAA 1 cut(s) 452
Psp5II RGGWCCY 1 cut(s) 375
PspN4I GGNNCC 1 cut(s) 376
PspPI GGNCC 5 cut(s) 130, 181, 375, 689, 773
PspPPI RGGWCCY 1 cut(s) 375
PsuI RGATCY 3 cut(s) 76, 350, 598
PvuII CAGCTG 1 cut(s) 565
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
RseI CAYNNNNRTG 2 cut(s) 442, 635
SapI GCTCTTC 2 cut(s) 387, 456
SaqAI TTAA 2 cut(s) 528, 797
SatI GCNGC 6 cut(s) 112, 141, 237, 248, 402, 522
Sau3AI GATC 6 cut(s) 60, 76, 350, 513, 598, 786
Sau96I GGNCC 5 cut(s) 130, 181, 375, 689, 773
ScrFI CCNGG 1 cut(s) 104
SduI GDGCHC 1 cut(s) 285
SfaNI GCATC 1 cut(s) 223
SfiI GGCCNNNNNGGCC 1 cut(s) 149
SinI GGWCC 3 cut(s) 181, 375, 689
SmiMI CAYNNNNRTG 2 cut(s) 442, 635
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
Sse9I AATT 2 cut(s) 257, 316
SsiI CCGC 4 cut(s) 65, 140, 184, 237
SspMI CTAG 1 cut(s) 203
StyD4I CCNGG 1 cut(s) 102
StyI CCWWGG 2 cut(s) 153, 745
TaaI ACNGT 3 cut(s) 56, 505, 733
TaiI ACGT 3 cut(s) 430, 545, 769
TaqI TCGA 5 cut(s) 135, 231, 308, 672, 781
TasI AATT 2 cut(s) 257, 316
TauI GCSGC 2 cut(s) 143, 239
TfiI GAWTC 2 cut(s) 32, 470
Tru1I TTAA 2 cut(s) 528, 797
Tru9I TTAA 2 cut(s) 528, 797
TscAI CASTG 1 cut(s) 718
TseFI GTSAC 3 cut(s) 115, 191, 740
TseI GCWGC 4 cut(s) 111, 247, 401, 521
Tsp45I GTSAC 3 cut(s) 115, 191, 740
TspDTI ATGAA 1 cut(s) 698
TspRI CASTG 1 cut(s) 718
VpaK11BI GGWCC 3 cut(s) 181, 375, 689
VspI ATTAAT 1 cut(s) 528
XapI RAATTY 1 cut(s) 316
XceI RCATGY 3 cut(s) 100, 271, 293
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.