Rmu_sc0006415.1_g000020

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006415.1
OG0001324
Physical Location & Seq
Forward (+)
106069 .. 108447
2379 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006415.1_g000020.1.cds

Sequence Viewer

Length: 492 bp
atggaggcggagaaagcaggggcgtcgttggactcgctgattgccgggttcaacagtcggatctcggagctccaagagctcgttatagcccgaaacatgtacccggccagcagcgtcactgatttgacggccgtcgacgccgcagtgaaggccatggagcttcaggtccaggccatcaaggaccgggttcgcgacgaaaccctagccatccccaaagcaaaaaaactgatcgatgcatcgctgaagcagcaaaggaaattgaaggatatgtcaatttatgcccccactcatgttcctgaaagaatgtcgatgttgaatttggatacaaatagatgtttgtttccagaaagctctcagcaaaacactggttctgggaccttcaagcttgaggaggatcctgcaccaccaccaccaccaccaccaaccaaggtgtcaggagatcgttgggaaaaggctctggtaagtgaacttaaaccaggttactgggtctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

163

Amino Acids

17.96

Weight (kDa)

5.91

Isoelectric Point (pI)

48.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 135
AccII CGCG 1 cut(s) 192
AciI CCGC 2 cut(s) 8, 141
AclWI GGATC 3 cut(s) 68, 389, 402
AcoI YGGCCR 2 cut(s) 105, 129
AcsI RAATTY 1 cut(s) 316
AcuI CTGAAG 2 cut(s) 146, 263
AcyI GRCGYC 2 cut(s) 23, 138
AfaI GTAC 1 cut(s) 101
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 4 cut(s) 52, 262, 316, 382
AjnI CCWGG 2 cut(s) 168, 475
AluBI AGCT 5 cut(s) 70, 79, 160, 351, 385
AluI AGCT 5 cut(s) 70, 79, 160, 351, 385
Alw21I GWGCWC 2 cut(s) 72, 81
AlwI GGATC 3 cut(s) 68, 389, 402
AoxI GGCC 4 cut(s) 105, 129, 150, 171
ApeKI GCWGC 2 cut(s) 111, 247
ApoI RAATTY 1 cut(s) 316
AspS9I GGNCC 3 cut(s) 166, 181, 375
AsuC2I CCSGG 3 cut(s) 46, 104, 185
AvaII GGWCC 3 cut(s) 166, 181, 375
BamHI GGATCC 1 cut(s) 394
BanII GRGCYC 2 cut(s) 72, 81
Bbv12I GWGCWC 2 cut(s) 72, 81
BbvI GCAGC 2 cut(s) 123, 259
BccI CCATC 2 cut(s) 182, 215
BceAI ACGGC 2 cut(s) 116, 144
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BciT130I CCWGG 2 cut(s) 170, 477
BciVI GTATCC 1 cut(s) 316
BcnI CCSGG 3 cut(s) 46, 104, 185
BfaI CTAG 1 cut(s) 203
BfuI GTATCC 1 cut(s) 316
BisI GCNGC 3 cut(s) 112, 141, 248
BlsI GCNGC 3 cut(s) 113, 142, 249
Bme1390I CCNGG 5 cut(s) 46, 104, 170, 185, 477
Bme18I GGWCC 3 cut(s) 166, 181, 375
BmgT120I GGNCC 3 cut(s) 166, 181, 375
BmiI GGNNCC 2 cut(s) 376, 396
BmrFI CCNGG 5 cut(s) 46, 104, 170, 185, 477
BmsI GCATC 2 cut(s) 223, 245
BoxI GACNNNNGTC 1 cut(s) 131
BpuEI CTTGAG 1 cut(s) 407
BpuMI CCSGG 3 cut(s) 46, 104, 185
Bsa29I ATCGAT 1 cut(s) 231
BsaHI GRCGYC 2 cut(s) 23, 138
BsaJI CCNNGG 2 cut(s) 153, 426
Bse1I ACTGG 2 cut(s) 370, 488
BseBI CCWGG 2 cut(s) 170, 477
BseCI ATCGAT 1 cut(s) 231
BseDI CCNNGG 2 cut(s) 153, 426
BseGI GGATG 1 cut(s) 207
BseMII CTCAG 1 cut(s) 368
BseNI ACTGG 2 cut(s) 370, 488
BseRI GAGGAG 1 cut(s) 404
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 2 cut(s) 123, 259
BsgI GTGCAG 1 cut(s) 384
Bsh1236I CGCG 1 cut(s) 192
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 4 cut(s) 107, 131, 152, 173
BshVI ATCGAT 1 cut(s) 231
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 2 cut(s) 72, 81
BsiSI CCGG 3 cut(s) 45, 104, 184
BslFI GGGAC 1 cut(s) 388
BsmFI GGGAC 1 cut(s) 388
BsnI GGCC 4 cut(s) 107, 131, 152, 173
Bsp1286I GDGCHC 2 cut(s) 72, 81
Bsp143I GATC 4 cut(s) 60, 228, 394, 439
Bsp19I CCATGG 1 cut(s) 153
Bsp68I TCGCGA 1 cut(s) 192
BspACI CCGC 2 cut(s) 8, 141
BspANI GGCC 4 cut(s) 107, 131, 152, 173
BspCNI CTCAG 1 cut(s) 367
BspDI ATCGAT 1 cut(s) 231
BspFNI CGCG 1 cut(s) 192
BspLI GGNNCC 2 cut(s) 376, 396
BspPI GGATC 3 cut(s) 68, 389, 402
BsrI ACTGG 2 cut(s) 370, 488
BssECI CCNNGG 2 cut(s) 153, 426
BssMI GATC 4 cut(s) 60, 228, 394, 439
BssNI GRCGYC 2 cut(s) 23, 138
BssT1I CCWWGG 2 cut(s) 153, 426
Bst2UI CCWGG 2 cut(s) 170, 477
Bst4CI ACNGT 1 cut(s) 56
BstACI GRCGYC 2 cut(s) 23, 138
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 1 cut(s) 354
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 1 cut(s) 207
BstFNI CGCG 1 cut(s) 192
BstKTI GATC 4 cut(s) 63, 231, 397, 442
BstMBI GATC 4 cut(s) 60, 228, 394, 439
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 5 cut(s) 14, 76, 137, 149, 247
BstNI CCWGG 2 cut(s) 170, 477
BstNSI RCATGY 1 cut(s) 100
BstPAI GACNNNNGTC 1 cut(s) 131
BstSCI CCNGG 5 cut(s) 44, 102, 168, 183, 475
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 2 cut(s) 123, 259
BstX2I RGATCY 2 cut(s) 60, 394
BstXI CCANNNNNNTGG 1 cut(s) 483
BstYI RGATCY 2 cut(s) 60, 394
BstZI CGGCCG 1 cut(s) 129
Bsu15I ATCGAT 1 cut(s) 231
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 4 cut(s) 107, 131, 152, 173
BsuTUI ATCGAT 1 cut(s) 231
BtgI CCRYGG 1 cut(s) 153
BtgZI GCGATG 1 cut(s) 222
BtsCI GGATG 1 cut(s) 207
BtsI GCAGTG 1 cut(s) 150
BtsIMutI CAGTG 3 cut(s) 117, 150, 363
BtuMI TCGCGA 1 cut(s) 192
Cac8I GCNNGC 1 cut(s) 109
Cfr13I GGNCC 3 cut(s) 166, 181, 375
ClaI ATCGAT 1 cut(s) 231
CseI GACGC 3 cut(s) 12, 103, 146
CsiI ACCWGGT 1 cut(s) 475
Csp6I GTAC 1 cut(s) 100
CviAII CATG 3 cut(s) 97, 154, 290
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 1 cut(s) 354
DpnI GATC 4 cut(s) 62, 230, 396, 441
DpnII GATC 4 cut(s) 60, 228, 394, 439
EaeI YGGCCR 2 cut(s) 105, 129
EagI CGGCCG 1 cut(s) 129
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 2 cut(s) 70, 79
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 2 cut(s) 153, 426
Eco24I GRGCYC 2 cut(s) 72, 81
Eco47I GGWCC 3 cut(s) 166, 181, 375
Eco52I CGGCCG 1 cut(s) 129
Eco53kI GAGCTC 2 cut(s) 70, 79
Eco57I CTGAAG 2 cut(s) 146, 263
EcoICRI GAGCTC 2 cut(s) 70, 79
EcoO109I RGGNCCY 1 cut(s) 375
EcoRII CCWGG 2 cut(s) 168, 475
EcoT14I CCWWGG 2 cut(s) 153, 426
EcoT22I ATGCAT 1 cut(s) 238
EcoT38I GRGCYC 2 cut(s) 72, 81
ErhI CCWWGG 2 cut(s) 153, 426
FaeI CATG 3 cut(s) 100, 157, 293
FaiI YATR 6 cut(s) 86, 98, 155, 269, 279, 291
FaqI GGGAC 1 cut(s) 388
FatI CATG 3 cut(s) 96, 153, 289
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 3 cut(s) 112, 141, 248
FokI GGATG 1 cut(s) 194
FriOI GRGCYC 2 cut(s) 72, 81
Fsp4HI GCNGC 3 cut(s) 112, 141, 248
FspBI CTAG 1 cut(s) 203
GluI GCNGC 3 cut(s) 112, 141, 248
HaeIII GGCC 4 cut(s) 107, 131, 152, 173
HapII CCGG 3 cut(s) 45, 104, 184
HgaI GACGC 3 cut(s) 12, 103, 146
Hin1I GRCGYC 2 cut(s) 23, 138
Hin1II CATG 3 cut(s) 100, 157, 293
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 1 cut(s) 32
HpaII CCGG 3 cut(s) 45, 104, 184
Hpy166II GTNNAC 2 cut(s) 136, 467
Hpy188I TCNGA 3 cut(s) 60, 67, 491
Hpy188III TCNNGA 4 cut(s) 191, 296, 344, 435
Hpy8I GTNNAC 2 cut(s) 136, 467
Hpy99I CGWCG 4 cut(s) 28, 137, 140, 197
HpyAV CCTTC 3 cut(s) 142, 256, 388
HpyCH4III ACNGT 1 cut(s) 56
HpyCH4V TGCA 2 cut(s) 236, 401
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 76, 137, 149, 247
HpyF3I CTNAG 1 cut(s) 354
Hsp92I GRCGYC 2 cut(s) 23, 138
Hsp92II CATG 3 cut(s) 100, 157, 293
Kzo9I GATC 4 cut(s) 60, 228, 394, 439
LmnI GCTCC 3 cut(s) 67, 75, 157
Lsp1109I GCAGC 2 cut(s) 123, 259
LweI GCATC 2 cut(s) 223, 245
MabI ACCWGGT 1 cut(s) 475
MaeI CTAG 1 cut(s) 203
MaeIII GTNAC 2 cut(s) 115, 479
MalI GATC 4 cut(s) 62, 230, 396, 441
MboI GATC 4 cut(s) 60, 228, 394, 439
MflI RGATCY 2 cut(s) 60, 394
MhlI GDGCHC 2 cut(s) 72, 81
MluCI AATT 3 cut(s) 257, 273, 316
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 2 cut(s) 9, 38
MnlI CCTC 2 cut(s) 382, 385
Mph1103I ATGCAT 1 cut(s) 238
MseI TTAA 1 cut(s) 471
MspI CCGG 3 cut(s) 45, 104, 184
MspR9I CCNGG 5 cut(s) 46, 104, 170, 185, 477
MvaI CCWGG 2 cut(s) 170, 477
MvnI CGCG 1 cut(s) 192
MwoI GCNNNNNNNGC 5 cut(s) 14, 76, 137, 149, 247
NciI CCSGG 3 cut(s) 46, 104, 185
NcoI CCATGG 1 cut(s) 153
NdeII GATC 4 cut(s) 60, 228, 394, 439
NlaIII CATG 3 cut(s) 100, 157, 293
NlaIV GGNNCC 2 cut(s) 376, 396
NmuCI GTSAC 1 cut(s) 115
NruI TCGCGA 1 cut(s) 192
NsiI ATGCAT 1 cut(s) 238
NspI RCATGY 1 cut(s) 100
PciI ACATGT 1 cut(s) 96
PkrI GCNGC 3 cut(s) 113, 142, 249
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PpuMI RGGWCCY 1 cut(s) 375
PscI ACATGT 1 cut(s) 96
PshAI GACNNNNGTC 1 cut(s) 131
Psp124BI GAGCTC 2 cut(s) 72, 81
Psp5II RGGWCCY 1 cut(s) 375
Psp6I CCWGG 2 cut(s) 168, 475
PspGI CCWGG 2 cut(s) 168, 475
PspN4I GGNNCC 2 cut(s) 376, 396
PspPI GGNCC 3 cut(s) 166, 181, 375
PspPPI RGGWCCY 1 cut(s) 375
PsuI RGATCY 2 cut(s) 60, 394
RruI TCGCGA 1 cut(s) 192
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SacI GAGCTC 2 cut(s) 72, 81
SalI GTCGAC 1 cut(s) 134
SaqAI TTAA 1 cut(s) 471
SatI GCNGC 3 cut(s) 112, 141, 248
Sau3AI GATC 4 cut(s) 60, 228, 394, 439
Sau96I GGNCC 3 cut(s) 166, 181, 375
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 5 cut(s) 46, 104, 170, 185, 477
SduI GDGCHC 2 cut(s) 72, 81
SetI ASST 9 cut(s) 72, 81, 162, 168, 353, 380, 387, 432, 481
SexAI ACCWGGT 1 cut(s) 475
SfaNI GCATC 2 cut(s) 223, 245
SgrDI CGTCGACG 1 cut(s) 134
SinI GGWCC 3 cut(s) 166, 181, 375
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
Sse9I AATT 3 cut(s) 257, 273, 316
SsiI CCGC 2 cut(s) 8, 141
SspMI CTAG 1 cut(s) 203
SstI GAGCTC 2 cut(s) 72, 81
StyD4I CCNGG 5 cut(s) 44, 102, 168, 183, 475
StyI CCWWGG 2 cut(s) 153, 426
TaaI ACNGT 1 cut(s) 56
TaqI TCGA 3 cut(s) 135, 231, 308
TasI AATT 3 cut(s) 257, 273, 316
TauI GCSGC 1 cut(s) 143
Tru1I TTAA 1 cut(s) 471
Tru9I TTAA 1 cut(s) 471
TscAI CASTG 3 cut(s) 124, 150, 370
TseFI GTSAC 1 cut(s) 115
TseI GCWGC 2 cut(s) 111, 247
Tsp45I GTSAC 1 cut(s) 115
TspRI CASTG 3 cut(s) 124, 150, 370
VpaK11BI GGWCC 3 cut(s) 166, 181, 375
XapI RAATTY 1 cut(s) 316
XceI RCATGY 1 cut(s) 100
XmiI GTMKAC 1 cut(s) 135
XspI CTAG 1 cut(s) 203
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.