RchiOBHm_Chr1g0349241

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001324
Physical Location & Seq
Forward (+)
42356655 .. 42357441
787 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57522

Sequence Viewer

Length: 342 bp
ATGGAGGCTGAGCAAGCAGGGGCGTCGTTGGACTCGCTGATCGCCGTGTTCAACAGTCGGATCTCGGAGCTCCAAGAGCTCGTCATTGCTCGAAACATGTACCCAGCCAGCAGCGTCACCGATTTGACGGCCGTCGAGGCCGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGCTATCAAGCACCGGGTTCGCGATGAAACCCTAGCCATCCCCAAAGCAAAAGTAAAACTGATCGATGCATCGCTGAAGCAGCAAAGGAAATTGAAGGATATGTCAGTTTATGCCCCCACTCATGTTCCGGAAAGGGTTTCAATGTTGAATTTGGATACAAACAGATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

113

Amino Acids

12.6

Weight (kDa)

8.03

Isoelectric Point (pI)

40.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 9 - 104 6.6e-24 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 192
AccIII TCCGGA 1 cut(s) 298
AciI CCGC 1 cut(s) 141
AclWI GGATC 1 cut(s) 68
AcoI YGGCCR 1 cut(s) 129
AcsI RAATTY 1 cut(s) 319
AcuI CTGAAG 2 cut(s) 146, 266
AcyI GRCGYC 1 cut(s) 23
AfaI GTAC 1 cut(s) 101
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 4 cut(s) 52, 265, 312, 319
AjnI CCWGG 1 cut(s) 168
AluBI AGCT 3 cut(s) 70, 79, 160
AluI AGCT 3 cut(s) 70, 79, 160
Alw21I GWGCWC 2 cut(s) 72, 81
AlwI GGATC 1 cut(s) 68
Aor13HI TCCGGA 1 cut(s) 298
AoxI GGCC 3 cut(s) 129, 138, 150
ApeKI GCWGC 2 cut(s) 111, 250
ApoI RAATTY 1 cut(s) 319
AspS9I GGNCC 1 cut(s) 166
AsuC2I CCSGG 1 cut(s) 185
AsuHPI GGTGA 1 cut(s) 109
AvaII GGWCC 1 cut(s) 166
BanII GRGCYC 2 cut(s) 72, 81
Bbv12I GWGCWC 2 cut(s) 72, 81
BbvI GCAGC 2 cut(s) 123, 262
BccI CCATC 2 cut(s) 215, 330
BceAI ACGGC 4 cut(s) 29, 116, 128, 144
BcgI CGANNNNNNTGC 4 cut(s) 6, 40, 170, 204
BciT130I CCWGG 1 cut(s) 170
BciVI GTATCC 1 cut(s) 319
BcnI CCSGG 1 cut(s) 185
BfaI CTAG 1 cut(s) 203
BfuI GTATCC 1 cut(s) 319
BglI GCCNNNNNGGC 2 cut(s) 137, 149
BisI GCNGC 3 cut(s) 112, 141, 251
BlpI GCTNAGC 1 cut(s) 9
BlsI GCNGC 3 cut(s) 113, 142, 252
Bme1390I CCNGG 2 cut(s) 170, 185
Bme18I GGWCC 1 cut(s) 166
BmgT120I GGNCC 1 cut(s) 166
BmrFI CCNGG 2 cut(s) 170, 185
BmsI GCATC 2 cut(s) 226, 248
BoxI GACNNNNGTC 1 cut(s) 131
Bpu1102I GCTNAGC 1 cut(s) 9
BpuMI CCSGG 1 cut(s) 185
Bsa29I ATCGAT 1 cut(s) 234
BsaHI GRCGYC 1 cut(s) 23
BsaJI CCNNGG 1 cut(s) 153
BsaWI WCCGGW 1 cut(s) 298
Bse3DI GCAATG 1 cut(s) 84
BseAI TCCGGA 1 cut(s) 298
BseBI CCWGG 1 cut(s) 170
BseCI ATCGAT 1 cut(s) 234
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 1 cut(s) 207
BseMI GCAATG 1 cut(s) 84
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 2 cut(s) 123, 262
BseYI CCCAGC 1 cut(s) 103
Bsh1236I CGCG 1 cut(s) 192
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 3 cut(s) 131, 140, 152
BshVI ATCGAT 1 cut(s) 234
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 2 cut(s) 72, 81
BsiSI CCGG 2 cut(s) 184, 299
BsnI GGCC 3 cut(s) 131, 140, 152
Bsp1286I GDGCHC 2 cut(s) 72, 81
Bsp13I TCCGGA 1 cut(s) 298
Bsp143I GATC 3 cut(s) 39, 60, 231
Bsp1720I GCTNAGC 1 cut(s) 9
Bsp19I CCATGG 1 cut(s) 153
Bsp68I TCGCGA 1 cut(s) 192
BspACI CCGC 1 cut(s) 141
BspANI GGCC 3 cut(s) 131, 140, 152
BspDI ATCGAT 1 cut(s) 234
BspEI TCCGGA 1 cut(s) 298
BspFNI CGCG 1 cut(s) 192
BspPI GGATC 1 cut(s) 68
BsrDI GCAATG 1 cut(s) 84
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 3 cut(s) 39, 60, 231
BssNI GRCGYC 1 cut(s) 23
BssT1I CCWWGG 1 cut(s) 153
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 1 cut(s) 56
BstACI GRCGYC 1 cut(s) 23
BstC8I GCNNGC 2 cut(s) 15, 109
BstDEI CTNAG 1 cut(s) 9
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 1 cut(s) 207
BstFNI CGCG 1 cut(s) 192
BstKTI GATC 3 cut(s) 42, 63, 234
BstMBI GATC 3 cut(s) 39, 60, 231
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 5 cut(s) 14, 76, 137, 149, 250
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 100
BstPAI GACNNNNGTC 1 cut(s) 131
BstSCI CCNGG 2 cut(s) 168, 183
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 2 cut(s) 123, 262
BstX2I RGATCY 1 cut(s) 60
BstYI RGATCY 1 cut(s) 60
BstZI CGGCCG 1 cut(s) 129
Bsu15I ATCGAT 1 cut(s) 234
BsuI GTATCC 1 cut(s) 319
BsuRI GGCC 3 cut(s) 131, 140, 152
BsuTUI ATCGAT 1 cut(s) 234
BtgI CCRYGG 1 cut(s) 153
BtgZI GCGATG 2 cut(s) 207, 225
BtsCI GGATG 1 cut(s) 207
BtuMI TCGCGA 1 cut(s) 192
Cac8I GCNNGC 2 cut(s) 15, 109
Cfr13I GGNCC 1 cut(s) 166
ClaI ATCGAT 1 cut(s) 234
CseI GACGC 2 cut(s) 12, 103
Csp6I GTAC 1 cut(s) 100
CviAII CATG 3 cut(s) 97, 154, 293
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 1 cut(s) 9
DpnI GATC 3 cut(s) 41, 62, 233
DpnII GATC 3 cut(s) 39, 60, 231
EaeI YGGCCR 1 cut(s) 129
EagI CGGCCG 1 cut(s) 129
Ecl136II GAGCTC 2 cut(s) 70, 79
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 153
Eco24I GRGCYC 2 cut(s) 72, 81
Eco47I GGWCC 1 cut(s) 166
Eco52I CGGCCG 1 cut(s) 129
Eco53kI GAGCTC 2 cut(s) 70, 79
Eco57I CTGAAG 2 cut(s) 146, 266
EcoICRI GAGCTC 2 cut(s) 70, 79
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 1 cut(s) 153
EcoT22I ATGCAT 1 cut(s) 241
EcoT38I GRGCYC 2 cut(s) 72, 81
ErhI CCWWGG 1 cut(s) 153
FaeI CATG 3 cut(s) 100, 157, 296
FaiI YATR 5 cut(s) 98, 155, 272, 282, 294
FatI CATG 3 cut(s) 96, 153, 292
Fnu4HI GCNGC 3 cut(s) 112, 141, 251
FokI GGATG 1 cut(s) 194
FriOI GRGCYC 2 cut(s) 72, 81
Fsp4HI GCNGC 3 cut(s) 112, 141, 251
FspBI CTAG 1 cut(s) 203
GluI GCNGC 3 cut(s) 112, 141, 251
GsaI CCCAGC 1 cut(s) 107
HaeIII GGCC 3 cut(s) 131, 140, 152
HapII CCGG 2 cut(s) 184, 299
HgaI GACGC 2 cut(s) 12, 103
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 3 cut(s) 100, 157, 296
HinfI GANTC 1 cut(s) 32
HpaII CCGG 2 cut(s) 184, 299
HphI GGTGA 1 cut(s) 109
Hpy188I TCNGA 2 cut(s) 60, 67
Hpy188III TCNNGA 2 cut(s) 191, 299
Hpy99I CGWCG 2 cut(s) 28, 137
HpyAV CCTTC 2 cut(s) 142, 259
HpyCH4III ACNGT 1 cut(s) 56
HpyCH4V TGCA 1 cut(s) 239
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 76, 137, 149, 250
HpyF3I CTNAG 1 cut(s) 9
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 3 cut(s) 100, 157, 296
Kpn2I TCCGGA 1 cut(s) 298
Kzo9I GATC 3 cut(s) 39, 60, 231
LmnI GCTCC 3 cut(s) 67, 75, 157
LpnPI CCDG 8 cut(s) 3, 117, 121, 149, 155, 182, 197, 312
Lsp1109I GCAGC 2 cut(s) 123, 262
LweI GCATC 2 cut(s) 226, 248
MaeI CTAG 1 cut(s) 203
MaeIII GTNAC 1 cut(s) 115
MalI GATC 3 cut(s) 41, 62, 233
MboI GATC 3 cut(s) 39, 60, 231
MflI RGATCY 1 cut(s) 60
MhlI GDGCHC 2 cut(s) 72, 81
MluCI AATT 2 cut(s) 260, 319
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 2 cut(s) 9, 38
MnlI CCTC 1 cut(s) 130
Mph1103I ATGCAT 1 cut(s) 241
MroI TCCGGA 1 cut(s) 298
MspI CCGG 2 cut(s) 184, 299
MspR9I CCNGG 2 cut(s) 170, 185
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 1 cut(s) 192
MwoI GCNNNNNNNGC 5 cut(s) 14, 76, 137, 149, 250
NciI CCSGG 1 cut(s) 185
NcoI CCATGG 1 cut(s) 153
NdeII GATC 3 cut(s) 39, 60, 231
NlaIII CATG 3 cut(s) 100, 157, 296
NmuCI GTSAC 1 cut(s) 115
NruI TCGCGA 1 cut(s) 192
NsiI ATGCAT 1 cut(s) 241
NspI RCATGY 1 cut(s) 100
PciI ACATGT 1 cut(s) 96
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PkrI GCNGC 3 cut(s) 113, 142, 252
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PscI ACATGT 1 cut(s) 96
PshAI GACNNNNGTC 1 cut(s) 131
Psp124BI GAGCTC 2 cut(s) 72, 81
Psp6I CCWGG 1 cut(s) 168
PspFI CCCAGC 1 cut(s) 103
PspGI CCWGG 1 cut(s) 168
PspPI GGNCC 1 cut(s) 166
PsuI RGATCY 1 cut(s) 60
RruI TCGCGA 1 cut(s) 192
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SacI GAGCTC 2 cut(s) 72, 81
SatI GCNGC 3 cut(s) 112, 141, 251
Sau3AI GATC 3 cut(s) 39, 60, 231
Sau96I GGNCC 1 cut(s) 166
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 2 cut(s) 170, 185
SduI GDGCHC 2 cut(s) 72, 81
SetI ASST 4 cut(s) 72, 81, 162, 168
SfaNI GCATC 2 cut(s) 226, 248
SfiI GGCCNNNNNGGCC 1 cut(s) 137
SinI GGWCC 1 cut(s) 166
Sse9I AATT 2 cut(s) 260, 319
SsiI CCGC 1 cut(s) 141
SspMI CTAG 1 cut(s) 203
SstI GAGCTC 2 cut(s) 72, 81
StyD4I CCNGG 2 cut(s) 168, 183
StyI CCWWGG 1 cut(s) 153
TaaI ACNGT 1 cut(s) 56
TaqI TCGA 3 cut(s) 91, 135, 234
TasI AATT 2 cut(s) 260, 319
TauI GCSGC 1 cut(s) 143
TseFI GTSAC 1 cut(s) 115
TseI GCWGC 2 cut(s) 111, 250
Tsp45I GTSAC 1 cut(s) 115
TspDTI ATGAA 1 cut(s) 210
VpaK11BI GGWCC 1 cut(s) 166
XapI RAATTY 1 cut(s) 319
XceI RCATGY 1 cut(s) 100
XspI CTAG 1 cut(s) 203
Zsp2I ATGCAT 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.