Rroxscaffold_4G00305780

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001324
Physical Location & Seq
Reverse (-)
26424122 .. 26427767
3646 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00305780.1

Sequence Viewer

Length: 765 bp
ATGCTTCTCATTTTCCCGCCAAAACAACTTTCCCAACTTCAAAACGAAAATCCACGAATCGAAGATCGAAACTCTCTGAAGAGAGAGAGGGAGAGAGCGATGGAGGCAGAGAAAGCAGGGGCGTCGTTGGACTCGCTGATCGCCGGGTTCAACAGTCGGGTCTCGGAGCTCCAAGGGCTTGTTATTGCGCGAAACATGTACCCGGCCAGCAGCGTCACCGATTTGACGGCCGTCGACGCAGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGCCATCAAGGACCGGGTTCGCGACGAAACCCTAGCTATCCCCAAAGCAAAAGTATGTGCGAGATGGTACAATGTGATGGCAGAAACTGATCGATGCATTGCTGAAGCAACAAAGGAAATTGAAGGATATGTCAGTTTATGCCCCCACTCATGTTCCGGAAAGGATGTCAATGTTGAATCTGGATACAAACAGATGGTAGAGAAAAAGGGTCGTTCCACCCCACCATTGTGGTTTATTAGTGCTGAAGAGCTGGATTCCTTGTCATCATACATGAGAGGAAGGCTTACACTAGAGAAGGTCAATGCAGCTATTAATGACATGGTGGCAGGAGATCGATGGGAAAAAGCCTTGGAAATAAGAGATATAGCAATGCATGAAGCAGTAAAGGGAAAACACTTCTTTCTCAAAACTGACATGAAAGGACCATCCTTGAAGCTTGACAACACTGGAAAAGCAATACTAAGTGTCAGGTTCTTCGTCACCTTGATCGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

254

Amino Acids

28.39

Weight (kDa)

8.26

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 42 - 246 1.8e-42 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 234
AccII CGCG 2 cut(s) 190, 291
AccIII TCCGGA 1 cut(s) 425
AciI CCGC 1 cut(s) 17
AcoI YGGCCR 2 cut(s) 204, 228
AcuI CTGAAG 4 cut(s) 98, 245, 393, 534
AcyI GRCGYC 1 cut(s) 122
AfaI GTAC 2 cut(s) 200, 338
AflIII ACRYGT 1 cut(s) 195
AgsI TTSAA 5 cut(s) 41, 151, 392, 446, 703
AjnI CCWGG 1 cut(s) 267
AjuI GAANNNNNNNTTGG 2 cut(s) 13, 45
AleI CACNNNNGTG 1 cut(s) 496
AluBI AGCT 6 cut(s) 169, 259, 305, 520, 578, 706
AluI AGCT 6 cut(s) 169, 259, 305, 520, 578, 706
Alw21I GWGCWC 1 cut(s) 171
Alw26I GTCTC 1 cut(s) 166
AlwNI CAGNNNCTG 1 cut(s) 356
Aor13HI TCCGGA 1 cut(s) 425
AoxI GGCC 4 cut(s) 204, 228, 249, 270
ApeKI GCWGC 3 cut(s) 210, 239, 575
AseI ATTAAT 1 cut(s) 582
AspLEI GCGC 1 cut(s) 190
AspS9I GGNCC 3 cut(s) 265, 280, 692
AsuC2I CCSGG 3 cut(s) 145, 203, 284
AsuHPI GGTGA 2 cut(s) 208, 742
AvaII GGWCC 3 cut(s) 265, 280, 692
BanII GRGCYC 1 cut(s) 171
Bbv12I GWGCWC 1 cut(s) 171
BbvI GCAGC 3 cut(s) 222, 251, 587
BccI CCATC 7 cut(s) 94, 281, 327, 340, 457, 600, 703
BceAI ACGGC 3 cut(s) 215, 227, 243
BcgI CGANNNNNNTGC 2 cut(s) 105, 139
BciT130I CCWGG 1 cut(s) 269
BciVI GTATCC 1 cut(s) 446
BcnI CCSGG 3 cut(s) 145, 203, 284
BcoDI GTCTC 1 cut(s) 166
BfaI CTAG 2 cut(s) 302, 560
BfuI GTATCC 1 cut(s) 446
BglI GCCNNNNNGGC 1 cut(s) 248
BisI GCNGC 3 cut(s) 211, 240, 576
BlsI GCNGC 3 cut(s) 212, 241, 577
Bme1390I CCNGG 4 cut(s) 145, 203, 269, 284
Bme18I GGWCC 3 cut(s) 265, 280, 692
BmgT120I GGNCC 3 cut(s) 265, 280, 692
BmrFI CCNGG 4 cut(s) 145, 203, 269, 284
BmsI GCATC 1 cut(s) 353
BoxI GACNNNNGTC 1 cut(s) 230
BpuMI CCSGG 3 cut(s) 145, 203, 284
Bsa29I ATCGAT 2 cut(s) 361, 604
BsaHI GRCGYC 1 cut(s) 122
BsaI GGTCTC 1 cut(s) 166
BsaJI CCNNGG 3 cut(s) 172, 252, 618
BsaWI WCCGGW 1 cut(s) 425
Bse1I ACTGG 1 cut(s) 721
Bse3DI GCAATG 2 cut(s) 366, 645
BseAI TCCGGA 1 cut(s) 425
BseBI CCWGG 1 cut(s) 269
BseCI ATCGAT 2 cut(s) 361, 604
BseDI CCNNGG 3 cut(s) 172, 252, 618
BseGI GGATG 2 cut(s) 439, 695
BseMI GCAATG 2 cut(s) 366, 645
BseNI ACTGG 1 cut(s) 721
BseX3I CGGCCG 1 cut(s) 228
BseXI GCAGC 3 cut(s) 222, 251, 587
Bsh1236I CGCG 2 cut(s) 190, 291
Bsh1285I CGRYCG 1 cut(s) 231
BshFI GGCC 4 cut(s) 206, 230, 251, 272
BshVI ATCGAT 2 cut(s) 361, 604
BsiEI CGRYCG 1 cut(s) 231
BsiHKAI GWGCWC 1 cut(s) 171
BsiSI CCGG 4 cut(s) 144, 203, 283, 426
BsmAI GTCTC 1 cut(s) 166
BsnI GGCC 4 cut(s) 206, 230, 251, 272
Bso31I GGTCTC 1 cut(s) 166
Bsp1286I GDGCHC 1 cut(s) 171
Bsp13I TCCGGA 1 cut(s) 425
Bsp143I GATC 5 cut(s) 64, 138, 358, 601, 756
Bsp19I CCATGG 1 cut(s) 252
Bsp68I TCGCGA 1 cut(s) 291
BspACI CCGC 1 cut(s) 17
BspANI GGCC 4 cut(s) 206, 230, 251, 272
BspDI ATCGAT 2 cut(s) 361, 604
BspEI TCCGGA 1 cut(s) 425
BspFNI CGCG 2 cut(s) 190, 291
BspQI GCTCTTC 1 cut(s) 510
BspTNI GGTCTC 1 cut(s) 166
BsrDI GCAATG 2 cut(s) 366, 645
BsrI ACTGG 1 cut(s) 721
BssECI CCNNGG 3 cut(s) 172, 252, 618
BssMI GATC 5 cut(s) 64, 138, 358, 601, 756
BssNI GRCGYC 1 cut(s) 122
BssT1I CCWWGG 3 cut(s) 172, 252, 618
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 1 cut(s) 155
Bst6I CTCTTC 2 cut(s) 74, 510
BstACI GRCGYC 1 cut(s) 122
BstC8I GCNNGC 1 cut(s) 208
BstDEI CTNAG 1 cut(s) 731
BstDSI CCRYGG 1 cut(s) 252
BstF5I GGATG 2 cut(s) 439, 695
BstFNI CGCG 2 cut(s) 190, 291
BstHHI GCGC 1 cut(s) 190
BstKTI GATC 5 cut(s) 67, 141, 361, 604, 759
BstMAI GTCTC 1 cut(s) 166
BstMBI GATC 5 cut(s) 64, 138, 358, 601, 756
BstMCI CGRYCG 1 cut(s) 231
BstMWI GCNNNNNNNGC 5 cut(s) 104, 113, 175, 236, 248
BstNI CCWGG 1 cut(s) 269
BstNSI RCATGY 1 cut(s) 199
BstPAI GACNNNNGTC 1 cut(s) 230
BstSCI CCNGG 4 cut(s) 143, 201, 267, 282
BstUI CGCG 2 cut(s) 190, 291
BstV1I GCAGC 3 cut(s) 222, 251, 587
BstXI CCANNNNNNTGG 1 cut(s) 498
BstZI CGGCCG 1 cut(s) 228
Bsu15I ATCGAT 2 cut(s) 361, 604
BsuI GTATCC 1 cut(s) 446
BsuRI GGCC 4 cut(s) 206, 230, 251, 272
BsuTUI ATCGAT 2 cut(s) 361, 604
BtgI CCRYGG 1 cut(s) 252
BtgZI GCGATG 1 cut(s) 113
BtsCI GGATG 2 cut(s) 439, 695
BtsIMutI CAGTG 1 cut(s) 714
BtuMI TCGCGA 1 cut(s) 291
Cac8I GCNNGC 1 cut(s) 208
CaiI CAGNNNCTG 1 cut(s) 356
CfoI GCGC 1 cut(s) 190
Cfr13I GGNCC 3 cut(s) 265, 280, 692
ClaI ATCGAT 2 cut(s) 361, 604
CseI GACGC 3 cut(s) 111, 202, 245
Csp6I GTAC 2 cut(s) 199, 337
CviAII CATG 7 cut(s) 196, 253, 420, 541, 589, 644, 685
CviQI GTAC 2 cut(s) 199, 337
DdeI CTNAG 1 cut(s) 731
DpnI GATC 5 cut(s) 66, 140, 360, 603, 758
DpnII GATC 5 cut(s) 64, 138, 358, 601, 756
EaeI YGGCCR 2 cut(s) 204, 228
EagI CGGCCG 1 cut(s) 228
Eam1104I CTCTTC 2 cut(s) 74, 510
EarI CTCTTC 2 cut(s) 74, 510
Ecl136II GAGCTC 1 cut(s) 169
EclXI CGGCCG 1 cut(s) 228
Eco130I CCWWGG 3 cut(s) 172, 252, 618
Eco24I GRGCYC 1 cut(s) 171
Eco31I GGTCTC 1 cut(s) 166
Eco47I GGWCC 3 cut(s) 265, 280, 692
Eco52I CGGCCG 1 cut(s) 228
Eco53kI GAGCTC 1 cut(s) 169
Eco57I CTGAAG 4 cut(s) 98, 245, 393, 534
EcoICRI GAGCTC 1 cut(s) 169
EcoRII CCWGG 1 cut(s) 267
EcoT14I CCWWGG 3 cut(s) 172, 252, 618
EcoT22I ATGCAT 2 cut(s) 368, 645
EcoT38I GRGCYC 1 cut(s) 171
ErhI CCWWGG 3 cut(s) 172, 252, 618
FaeI CATG 7 cut(s) 199, 256, 423, 544, 592, 647, 688
FatI CATG 7 cut(s) 195, 252, 419, 540, 588, 643, 684
FauI CCCGC 1 cut(s) 24
FblI GTMKAC 1 cut(s) 234
Fnu4HI GCNGC 3 cut(s) 211, 240, 576
FokI GGATG 2 cut(s) 446, 682
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 3 cut(s) 211, 240, 576
FspBI CTAG 2 cut(s) 302, 560
GlaI GCGC 1 cut(s) 189
GluI GCNGC 3 cut(s) 211, 240, 576
HaeIII GGCC 4 cut(s) 206, 230, 251, 272
HapII CCGG 4 cut(s) 144, 203, 283, 426
HgaI GACGC 3 cut(s) 111, 202, 245
HhaI GCGC 1 cut(s) 190
Hin1I GRCGYC 1 cut(s) 122
Hin1II CATG 7 cut(s) 199, 256, 423, 544, 592, 647, 688
Hin6I GCGC 1 cut(s) 188
HinP1I GCGC 1 cut(s) 188
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HindIII AAGCTT 1 cut(s) 704
HinfI GANTC 4 cut(s) 57, 131, 446, 524
HpaII CCGG 4 cut(s) 144, 203, 283, 426
HphI GGTGA 2 cut(s) 208, 742
Hpy166II GTNNAC 1 cut(s) 235
Hpy188I TCNGA 2 cut(s) 78, 166
Hpy188III TCNNGA 3 cut(s) 290, 426, 450
Hpy8I GTNNAC 1 cut(s) 235
Hpy99I CGWCG 4 cut(s) 127, 236, 239, 296
HpyAV CCTTC 4 cut(s) 241, 386, 543, 559
HpyCH4III ACNGT 1 cut(s) 155
HpyCH4V TGCA 3 cut(s) 366, 575, 643
HpyF10VI GCNNNNNNNGC 5 cut(s) 104, 113, 175, 236, 248
HpyF3I CTNAG 1 cut(s) 731
Hsp92I GRCGYC 1 cut(s) 122
Hsp92II CATG 7 cut(s) 199, 256, 423, 544, 592, 647, 688
HspAI GCGC 1 cut(s) 188
Kpn2I TCCGGA 1 cut(s) 425
Kzo9I GATC 5 cut(s) 64, 138, 358, 601, 756
LguI GCTCTTC 1 cut(s) 510
LmnI GCTCC 3 cut(s) 166, 174, 256
Lsp1109I GCAGC 3 cut(s) 222, 251, 587
LweI GCATC 1 cut(s) 353
MaeI CTAG 2 cut(s) 302, 560
MaeIII GTNAC 2 cut(s) 214, 748
MalI GATC 5 cut(s) 66, 140, 360, 603, 758
MboI GATC 5 cut(s) 64, 138, 358, 601, 756
MboII GAAGA 4 cut(s) 74, 91, 527, 736
MhlI GDGCHC 1 cut(s) 171
MluCI AATT 1 cut(s) 387
MlyI GAGTC 1 cut(s) 125
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 3 cut(s) 81, 97, 539
Mph1103I ATGCAT 2 cut(s) 368, 645
MroI TCCGGA 1 cut(s) 425
MseI TTAA 1 cut(s) 582
MslI CAYNNNNRTG 1 cut(s) 496
MspI CCGG 4 cut(s) 144, 203, 283, 426
MspR9I CCNGG 4 cut(s) 145, 203, 269, 284
MvaI CCWGG 1 cut(s) 269
MvnI CGCG 2 cut(s) 190, 291
MwoI GCNNNNNNNGC 5 cut(s) 104, 113, 175, 236, 248
NciI CCSGG 3 cut(s) 145, 203, 284
NcoI CCATGG 1 cut(s) 252
NdeII GATC 5 cut(s) 64, 138, 358, 601, 756
NlaIII CATG 7 cut(s) 199, 256, 423, 544, 592, 647, 688
NmuCI GTSAC 2 cut(s) 214, 748
NruI TCGCGA 1 cut(s) 291
NsiI ATGCAT 2 cut(s) 368, 645
NspI RCATGY 1 cut(s) 199
OliI CACNNNNGTG 1 cut(s) 496
PciI ACATGT 1 cut(s) 195
PciSI GCTCTTC 1 cut(s) 510
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PfeI GAWTC 3 cut(s) 57, 446, 524
PkrI GCNGC 3 cut(s) 212, 241, 577
PleI GAGTC 1 cut(s) 125
PpsI GAGTC 1 cut(s) 125
PscI ACATGT 1 cut(s) 195
PshAI GACNNNNGTC 1 cut(s) 230
PshBI ATTAAT 1 cut(s) 582
Psp124BI GAGCTC 1 cut(s) 171
Psp6I CCWGG 1 cut(s) 267
PspGI CCWGG 1 cut(s) 267
PspPI GGNCC 3 cut(s) 265, 280, 692
PstNI CAGNNNCTG 1 cut(s) 356
RruI TCGCGA 1 cut(s) 291
RsaI GTAC 2 cut(s) 200, 338
RsaNI GTAC 2 cut(s) 199, 337
RseI CAYNNNNRTG 1 cut(s) 496
SacI GAGCTC 1 cut(s) 171
SalI GTCGAC 1 cut(s) 233
SapI GCTCTTC 1 cut(s) 510
SaqAI TTAA 1 cut(s) 582
SatI GCNGC 3 cut(s) 211, 240, 576
Sau3AI GATC 5 cut(s) 64, 138, 358, 601, 756
Sau96I GGNCC 3 cut(s) 265, 280, 692
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 4 cut(s) 145, 203, 269, 284
SduI GDGCHC 1 cut(s) 171
SfaNI GCATC 1 cut(s) 353
SgrDI CGTCGACG 1 cut(s) 233
SinI GGWCC 3 cut(s) 265, 280, 692
SmiMI CAYNNNNRTG 1 cut(s) 496
Sse9I AATT 1 cut(s) 387
SsiI CCGC 1 cut(s) 17
SspMI CTAG 2 cut(s) 302, 560
SstI GAGCTC 1 cut(s) 171
StyD4I CCNGG 4 cut(s) 143, 201, 267, 282
StyI CCWWGG 3 cut(s) 172, 252, 618
TaaI ACNGT 1 cut(s) 155
TaqI TCGA 5 cut(s) 60, 67, 234, 361, 604
TasI AATT 1 cut(s) 387
TfiI GAWTC 3 cut(s) 57, 446, 524
Tru1I TTAA 1 cut(s) 582
Tru9I TTAA 1 cut(s) 582
TscAI CASTG 1 cut(s) 721
TseFI GTSAC 2 cut(s) 214, 748
TseI GCWGC 3 cut(s) 210, 239, 575
Tsp45I GTSAC 2 cut(s) 214, 748
TspDTI ATGAA 2 cut(s) 660, 701
TspRI CASTG 1 cut(s) 721
VpaK11BI GGWCC 3 cut(s) 265, 280, 692
VspI ATTAAT 1 cut(s) 582
XceI RCATGY 1 cut(s) 199
XmiI GTMKAC 1 cut(s) 234
XspI CTAG 2 cut(s) 302, 560
Zsp2I ATGCAT 2 cut(s) 368, 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.