RLG00000028593

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001324
Physical Location & Seq
Reverse (-)
24807751 .. 24813382
5632 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028593

Sequence Viewer

Length: 660 bp
ATGGACTACATCCAGATCGACGCTGATGAAGTCCCGATGACTCTTGAGAATACCCAAACTCAATATTCCGATTCCGATTGCGATAGCCTCCATTCCGAATCGAATTCCTGTTCTTCTATTGAAATTGAAGTTGATCAGGATTCCGAGTCCTATTGTGAATCGGAGTCGCCAGTTTCTGATCCATGTGGGGCTTTCACTGCAGATGAAATGATGACTAGAGTGGAGGGTATGGAGCGTGAGACTATTACGAAGAGCTTCGTTTCCGTTATGAAAGCCACCGACGGAGAAACCAGAGTGGTGGCTATGCATAAGAATTCGATGTACCCGGCCAGCAGCATCACCGATTTGACGGCTGTCGCCGCCGCCGTGAAGGCCATGGAGCTTCAGGTCCAGGCCATCAAGGACTGGAAACTGATCGATGCATCCCTGAAGCAGCAAAGGAAATTGAAGGATATGTCAATTTATGACCCCGCTCATGTTCCTGAAAGAATGTTGATGTTGAATTTGGATACCAATAGATGTTTATTTCCAGAAAGCTCTCAACAAAACACTGGTTCTGGGACCTTCAAGCTTGAGGAGGATCCTGCACCACTACCACCACCACCCAAGGAGAAAAAGGGTCGTTCCACGCCACCCTTGTGGTTTTTGAGTGCTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

220

Amino Acids

24.36

Weight (kDa)

4.48

Isoelectric Point (pI)

54.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 102 - 218 5e-09 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 473
AciI CCGC 3 cut(s) 360, 363, 471
AclWI GGATC 3 cut(s) 173, 575, 588
AcoI YGGCCR 1 cut(s) 327
AcsI RAATTY 3 cut(s) 103, 313, 502
AcuI CTGAAG 2 cut(s) 368, 449
AfaI GTAC 1 cut(s) 323
AgsI TTSAA 5 cut(s) 122, 128, 448, 502, 568
AjnI CCWGG 1 cut(s) 390
AleI CACNNNNGTG 1 cut(s) 637
AluBI AGCT 4 cut(s) 255, 382, 537, 571
AluI AGCT 4 cut(s) 255, 382, 537, 571
Alw26I GTCTC 1 cut(s) 233
AlwI GGATC 3 cut(s) 173, 575, 588
AlwNI CAGNNNCTG 1 cut(s) 176
AoxI GGCC 3 cut(s) 327, 372, 393
ApeKI GCWGC 2 cut(s) 333, 433
ApoI RAATTY 3 cut(s) 103, 313, 502
Asp700I GAANNNNTTC 1 cut(s) 254
AspS9I GGNCC 2 cut(s) 388, 561
AsuC2I CCSGG 1 cut(s) 326
AsuHPI GGTGA 1 cut(s) 331
AvaII GGWCC 2 cut(s) 388, 561
BamHI GGATCC 1 cut(s) 580
BbvI GCAGC 2 cut(s) 345, 445
BccI CCATC 1 cut(s) 404
BceAI ACGGC 2 cut(s) 350, 366
BciT130I CCWGG 1 cut(s) 392
BciVI GTATCC 1 cut(s) 502
BclI TGATCA 1 cut(s) 133
BcnI CCSGG 1 cut(s) 326
BcoDI GTCTC 1 cut(s) 233
BfaI CTAG 1 cut(s) 216
BfmI CTRYAG 1 cut(s) 198
BfuI GTATCC 1 cut(s) 502
BglI GCCNNNNNGGC 1 cut(s) 371
BisI GCNGC 4 cut(s) 334, 360, 363, 434
BlsI GCNGC 4 cut(s) 335, 361, 364, 435
Bme1390I CCNGG 2 cut(s) 326, 392
Bme18I GGWCC 2 cut(s) 388, 561
BmgT120I GGNCC 2 cut(s) 388, 561
BmiI GGNNCC 2 cut(s) 562, 582
BmrFI CCNGG 2 cut(s) 326, 392
BmsI GCATC 3 cut(s) 345, 409, 431
BoxI GACNNNNGTC 1 cut(s) 353
BpuEI CTTGAG 2 cut(s) 65, 593
BpuMI CCSGG 1 cut(s) 326
Bsa29I ATCGAT 1 cut(s) 417
BsaJI CCNNGG 2 cut(s) 375, 606
Bse1I ACTGG 3 cut(s) 170, 410, 556
BseBI CCWGG 1 cut(s) 392
BseCI ATCGAT 1 cut(s) 417
BseDI CCNNGG 2 cut(s) 375, 606
BseGI GGATG 2 cut(s) 9, 422
BseNI ACTGG 3 cut(s) 170, 410, 556
BseRI GAGGAG 1 cut(s) 590
BseXI GCAGC 2 cut(s) 345, 445
BsgI GTGCAG 1 cut(s) 570
BshFI GGCC 3 cut(s) 329, 374, 395
BshVI ATCGAT 1 cut(s) 417
BsiSI CCGG 1 cut(s) 326
BslFI GGGAC 2 cut(s) 17, 574
BsmAI GTCTC 1 cut(s) 233
BsmFI GGGAC 2 cut(s) 17, 574
BsnI GGCC 3 cut(s) 329, 374, 395
Bsp143I GATC 5 cut(s) 15, 133, 178, 414, 580
Bsp19I CCATGG 1 cut(s) 375
BspACI CCGC 3 cut(s) 360, 363, 471
BspANI GGCC 3 cut(s) 329, 374, 395
BspDI ATCGAT 1 cut(s) 417
BspLI GGNNCC 2 cut(s) 562, 582
BspMAI CTGCAG 1 cut(s) 202
BspPI GGATC 3 cut(s) 173, 575, 588
BspQI GCTCTTC 1 cut(s) 245
BsrBI CCGCTC 1 cut(s) 473
BsrI ACTGG 3 cut(s) 170, 410, 556
BssECI CCNNGG 2 cut(s) 375, 606
BssMI GATC 5 cut(s) 15, 133, 178, 414, 580
BssT1I CCWWGG 2 cut(s) 375, 606
Bst2UI CCWGG 1 cut(s) 392
Bst6I CTCTTC 1 cut(s) 245
BstC8I GCNNGC 1 cut(s) 331
BstDSI CCRYGG 1 cut(s) 375
BstF5I GGATG 2 cut(s) 9, 422
BstKTI GATC 5 cut(s) 18, 136, 181, 417, 583
BstMAI GTCTC 1 cut(s) 233
BstMBI GATC 5 cut(s) 15, 133, 178, 414, 580
BstMWI GCNNNNNNNGC 3 cut(s) 197, 359, 371
BstNI CCWGG 1 cut(s) 392
BstPAI GACNNNNGTC 1 cut(s) 353
BstSCI CCNGG 2 cut(s) 324, 390
BstSFI CTRYAG 1 cut(s) 198
BstV1I GCAGC 2 cut(s) 345, 445
BstX2I RGATCY 1 cut(s) 580
BstXI CCANNNNNNTGG 2 cut(s) 298, 639
BstYI RGATCY 1 cut(s) 580
Bsu15I ATCGAT 1 cut(s) 417
BsuI GTATCC 1 cut(s) 502
BsuRI GGCC 3 cut(s) 329, 374, 395
BsuTUI ATCGAT 1 cut(s) 417
BtgI CCRYGG 1 cut(s) 375
BtsCI GGATG 2 cut(s) 9, 422
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 2 cut(s) 195, 549
Cac8I GCNNGC 1 cut(s) 331
CaiI CAGNNNCTG 1 cut(s) 176
Cfr13I GGNCC 2 cut(s) 388, 561
ClaI ATCGAT 1 cut(s) 417
CseI GACGC 1 cut(s) 29
Csp6I GTAC 1 cut(s) 322
CviAII CATG 3 cut(s) 183, 376, 476
CviQI GTAC 1 cut(s) 322
DpnI GATC 5 cut(s) 17, 135, 180, 416, 582
DpnII GATC 5 cut(s) 15, 133, 178, 414, 580
EaeI YGGCCR 1 cut(s) 327
Eam1104I CTCTTC 1 cut(s) 245
EarI CTCTTC 1 cut(s) 245
Eco130I CCWWGG 2 cut(s) 375, 606
Eco47I GGWCC 2 cut(s) 388, 561
Eco57I CTGAAG 2 cut(s) 368, 449
EcoO109I RGGNCCY 1 cut(s) 561
EcoRI GAATTC 2 cut(s) 103, 313
EcoRII CCWGG 1 cut(s) 390
EcoT14I CCWWGG 2 cut(s) 375, 606
EcoT22I ATGCAT 2 cut(s) 309, 424
ErhI CCWWGG 2 cut(s) 375, 606
FaeI CATG 3 cut(s) 186, 379, 479
FaiI YATR 9 cut(s) 184, 230, 269, 305, 309, 377, 455, 465, 477
FaqI GGGAC 2 cut(s) 17, 574
FatI CATG 3 cut(s) 182, 375, 475
FauI CCCGC 1 cut(s) 478
FbaI TGATCA 1 cut(s) 133
Fnu4HI GCNGC 4 cut(s) 334, 360, 363, 434
FokI GGATG 1 cut(s) 409
Fsp4HI GCNGC 4 cut(s) 334, 360, 363, 434
FspBI CTAG 1 cut(s) 216
GluI GCNGC 4 cut(s) 334, 360, 363, 434
HaeIII GGCC 3 cut(s) 329, 374, 395
HapII CCGG 1 cut(s) 326
HgaI GACGC 1 cut(s) 29
Hin1II CATG 3 cut(s) 186, 379, 479
HindIII AAGCTT 1 cut(s) 569
HinfI GANTC 7 cut(s) 40, 71, 98, 140, 146, 158, 164
HpaII CCGG 1 cut(s) 326
HphI GGTGA 1 cut(s) 331
Hpy188I TCNGA 6 cut(s) 70, 76, 97, 145, 163, 178
Hpy188III TCNNGA 6 cut(s) 13, 34, 44, 137, 482, 530
Hpy99I CGWCG 2 cut(s) 23, 284
HpyAV CCTTC 3 cut(s) 364, 442, 574
HpyCH4V TGCA 4 cut(s) 200, 307, 422, 587
HpyF10VI GCNNNNNNNGC 3 cut(s) 197, 359, 371
Hsp92II CATG 3 cut(s) 186, 379, 479
Ksp22I TGATCA 1 cut(s) 133
Kzo9I GATC 5 cut(s) 15, 133, 178, 414, 580
LguI GCTCTTC 1 cut(s) 245
LmnI GCTCC 2 cut(s) 232, 379
Lsp1109I GCAGC 2 cut(s) 345, 445
LweI GCATC 3 cut(s) 345, 409, 431
MaeI CTAG 1 cut(s) 216
MalI GATC 5 cut(s) 17, 135, 180, 416, 582
MbiI CCGCTC 1 cut(s) 473
MboI GATC 5 cut(s) 15, 133, 178, 414, 580
MboII GAAGA 2 cut(s) 105, 262
MflI RGATCY 1 cut(s) 580
MluCI AATT 6 cut(s) 103, 123, 313, 443, 459, 502
MlyI GAGTC 3 cut(s) 34, 155, 173
MnlI CCTC 4 cut(s) 98, 217, 568, 571
Mph1103I ATGCAT 2 cut(s) 309, 424
MroXI GAANNNNTTC 1 cut(s) 254
MslI CAYNNNNRTG 1 cut(s) 637
MspI CCGG 1 cut(s) 326
MspR9I CCNGG 2 cut(s) 326, 392
MvaI CCWGG 1 cut(s) 392
MwoI GCNNNNNNNGC 3 cut(s) 197, 359, 371
NciI CCSGG 1 cut(s) 326
NcoI CCATGG 1 cut(s) 375
NdeII GATC 5 cut(s) 15, 133, 178, 414, 580
NlaIII CATG 3 cut(s) 186, 379, 479
NlaIV GGNNCC 2 cut(s) 562, 582
NsiI ATGCAT 2 cut(s) 309, 424
OliI CACNNNNGTG 1 cut(s) 637
PciSI GCTCTTC 1 cut(s) 245
PcsI WCGNNNNNNNCGW 1 cut(s) 363
PdmI GAANNNNTTC 1 cut(s) 254
PfeI GAWTC 4 cut(s) 71, 98, 140, 158
PkrI GCNGC 4 cut(s) 335, 361, 364, 435
PleI GAGTC 3 cut(s) 34, 154, 172
PpsI GAGTC 3 cut(s) 34, 154, 172
PpuMI RGGWCCY 1 cut(s) 561
PshAI GACNNNNGTC 1 cut(s) 353
Psp5II RGGWCCY 1 cut(s) 561
Psp6I CCWGG 1 cut(s) 390
PspGI CCWGG 1 cut(s) 390
PspN4I GGNNCC 2 cut(s) 562, 582
PspPI GGNCC 2 cut(s) 388, 561
PspPPI RGGWCCY 1 cut(s) 561
PstI CTGCAG 1 cut(s) 202
PstNI CAGNNNCTG 1 cut(s) 176
PsuI RGATCY 1 cut(s) 580
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
RseI CAYNNNNRTG 1 cut(s) 637
SapI GCTCTTC 1 cut(s) 245
SatI GCNGC 4 cut(s) 334, 360, 363, 434
Sau3AI GATC 5 cut(s) 15, 133, 178, 414, 580
Sau96I GGNCC 2 cut(s) 388, 561
SchI GAGTC 3 cut(s) 34, 155, 173
ScrFI CCNGG 2 cut(s) 326, 392
SetI ASST 6 cut(s) 257, 384, 390, 539, 566, 573
SfaNI GCATC 3 cut(s) 345, 409, 431
SfcI CTRYAG 1 cut(s) 198
SinI GGWCC 2 cut(s) 388, 561
SmiMI CAYNNNNRTG 1 cut(s) 637
SmlI CTYRAG 2 cut(s) 44, 572
SmoI CTYRAG 2 cut(s) 44, 572
Sse9I AATT 6 cut(s) 103, 123, 313, 443, 459, 502
SsiI CCGC 3 cut(s) 360, 363, 471
SspI AATATT 1 cut(s) 65
SspMI CTAG 1 cut(s) 216
StyD4I CCNGG 2 cut(s) 324, 390
StyI CCWWGG 2 cut(s) 375, 606
TaqI TCGA 4 cut(s) 18, 101, 317, 417
TasI AATT 6 cut(s) 103, 123, 313, 443, 459, 502
TauI GCSGC 2 cut(s) 362, 365
TfiI GAWTC 4 cut(s) 71, 98, 140, 158
TscAI CASTG 2 cut(s) 202, 556
TseI GCWGC 2 cut(s) 333, 433
TspDTI ATGAA 3 cut(s) 42, 219, 284
TspGWI ACGGA 2 cut(s) 253, 297
TspRI CASTG 2 cut(s) 202, 556
VpaK11BI GGWCC 2 cut(s) 388, 561
XapI RAATTY 3 cut(s) 103, 313, 502
XmnI GAANNNNTTC 1 cut(s) 254
XspI CTAG 1 cut(s) 216
Zsp2I ATGCAT 2 cut(s) 309, 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.