Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0001024

Physical Location & Seq

Genomic Coordinates Forward (+)

2352436 .. 2354338

Gene Structure 1903 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G07430.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1086 bp

ATGGGTTACATTTCAATGTCCGTGGTTGCATTCCTTGTTGTTTTTGCTTCGCCGGTGGTTCTAGCCACCGATACAGATCCGATTCCAGAAAACAGAGCCCAAATCCCGCAATGGTTTAAAACAAACGTTAAACCATATTCCCAGAGAAAGGGCACGCTAGACCCCGCCCTCGAAGCTGCTGAAGCAGCGCGACAAATTATCACCGTGAATCAAAAAGGTGGCGCTAATTTCAAGACATTAAACGAAGCTATCAAGAGCATTCCGACAGGAAACAAGAACCGTGTGATCATCAAGTTGGCTCCTGGTGTATACAATGAGAAGGTCACAATCGACATAGCTCGACCATTCATTACATTGCTTGGTCAACCTGGTGCGGAAACGGTCTTGACTTACCACGGGACAGCCGCTCAGTACGGAACCGTTGAGAGTGCAACTCTTATCGTCTGGGCTGAATATTTCCAGGCAGCTCACCTCACCATTAAAAACACTGCTCCAATGCCGAAACCAGGTTCACAGGGACAAGCTCTAGCAATGAGGATCAACGCTGATAAGGCAGCTTTCTATAGTTGTAGATTCCATGGTTTTCAAGATACTCTCTGTGACGACAAGGGCAACCATTTCTTCAAGGATTGTTACATCGAAGGAACTTATGATTTTATCTTTGGAAGAGGAGCTTCCTTGTACTTGAATACGCAATTGCACGCTGTTGGAGATGGATTAAGAGTGATCACAGCTCAAGGTAGACAAAGTGCAACCGAACAAAACGGATACACGTTCGTGCACTGCAAAGTCACTGGAACCGGAACCGGAATCTATTTGGGCAGGTCTTGGATGAGCCACCCTAAAGTCGTCTATGCTTTCACAGAGATGACCAGCGTGGTCAATCCATCTGGCTGGAGGGAGAACCTCAACCGCGGATATGACAAGACGGTGTTCTATGGAGAATACAAGTGTTTCGGACCAGGGTCACACTTAGAGAAGAGAGTGCCTTACACACAAGATATTGACAAAAACGAAGTTACGCCTTTCCTCACTCTTGGTTACATTAAGGGCTCCACATGGCTACTTCCTCCTCCTAAATACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0012505 GO:0016043 GO:0016049 GO:0016787 GO:0016788 GO:0022414 GO:0030154 GO:0030312 GO:0030599 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0042742 GO:0042995 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0048046 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0050829 GO:0050896 GO:0051704 GO:0051707 GO:0052689 GO:0060560 GO:0071840 GO:0071944 GO:0090406 GO:0098542 GO:0120025

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

Pectinesterase

Protein Analysis

361

Amino Acids

39.91

Weight (kDa)

8.98

Isoelectric Point (pI)

21.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pectinesterase	PF01095	67 - 351	2.9e-60	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001024)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69940 AT5G07410 AT5G07420 AT5G07430 AT5G61680
fragaria_vesca	FvH4_1g09290 FvH4_1g11590 FvH4_7g26440
malus_domestica	MD01G1173100.v1.1 MD01G1173200.v1.1 MD02G1100100.v1.1 MD02G1142600.v1.1 MD06G1150400.v1.1 MD07G1239900.v1.1
prunus_persica	Prupe.1G537900_v2.0.a1 Prupe.1G538100_v2.0.a1 Prupe.1G538100_v2.0.a1 Prupe.2G268200_v2.0.a1 Prupe.2G268300_v2.0.a1 Prupe.2G268500_v2.0.a1 Prupe.3G147000_v2.0.a1 Prupe.3G149100_v2.0.a1 Prupe.6G128000_v2.0.a1 Prupe.6G128100_v2.0.a1 Prupe.6G128200_v2.0.a1 Prupe.6G128300_v2.0.a1 Prupe.7G011200_v2.0.a1 Prupe.7G192800_v2.0.a1 Prupe.I005000_v2.0.a1 Prupe.I005200_v2.0.a1
pyrus_communis	pycom07g21580
rosa_chinensis	RchiOBHm_Chr1g0373011 RchiOBHm_Chr1g0373031 RchiOBHm_Chr2g0095851
rosa_laevigata	RLG00000016600 RLG00000026857 RLG00000026858
rosa_multiflora	Rmu_co8342511.1_g000001 Rmu_co8345149.1_g000001 Rmu_sc0000496.1_g000005 Rmu_ssc0000425.1_g000010 Rmu_ssc0000425.1_g000011
rosa_roxburghii	Rroxscaffold_2G00146030 Rroxscaffold_4G00284530 Rroxscaffold_4G00284540
rosa_rugosa	Rorug01G0375500 Rorug02G0053300
rosa_samantha	Rh1AG383800 Rh1BG347700 Rh1BG347800 Rh1CG361100 Rh1CG361200 Rh1DG378600 Rh1DG378700 Rh2AG102400 Rh2BG103800 Rh2CG106300 Rh2DG103900
rosa_wichuraiana	Rw1G033830 Rw1G033840 Rw2G007790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	813
AccBSI	CCGCTC	1 cut(s)	407
AccI	GTMKAC	2 cut(s)	309, 742
AccII	CGCG	2 cut(s)	190, 915
AciI	CCGC	6 cut(s)	107, 165, 374, 405, 913, 915
AclI	AACGTT	1 cut(s)	126
AclWI	GGATC	2 cut(s)	71, 545
AcuI	CTGAAG	1 cut(s)	201
AfaI	GTAC	2 cut(s)	413, 683
AfiI	CCNNNNNNNGG	2 cut(s)	148, 506
AflIII	ACRYGT	1 cut(s)	771
AgsI	TTSAA	5 cut(s)	15, 232, 587, 625, 688
AjnI	CCWGG	5 cut(s)	301, 367, 459, 505, 961
AleI	CACNNNNGTG	1 cut(s)	776
AluBI	AGCT	8 cut(s)	176, 248, 338, 467, 524, 557, 674, 734
AluI	AGCT	8 cut(s)	176, 248, 338, 467, 524, 557, 674, 734
Alw21I	GWGCWC	1 cut(s)	783
Alw44I	GTGCAC	1 cut(s)	779
AlwI	GGATC	2 cut(s)	71, 545
ApaLI	GTGCAC	1 cut(s)	779
ApeKI	GCWGC	4 cut(s)	176, 185, 464, 554
AspLEI	GCGC	2 cut(s)	190, 224
AspS9I	GGNCC	1 cut(s)	959
AsuHPI	GGTGA	3 cut(s)	193, 461, 466
AvaII	GGWCC	1 cut(s)	959
BaeGI	GKGCMC	2 cut(s)	155, 783
BanII	GRGCYC	2 cut(s)	100, 1055
Bbv12I	GWGCWC	1 cut(s)	783
BbvI	GCAGC	4 cut(s)	163, 197, 476, 566
BccI	CCATC	2 cut(s)	707, 895
BciT130I	CCWGG	5 cut(s)	303, 369, 461, 507, 963
BciVI	GTATCC	1 cut(s)	761
BclI	TGATCA	2 cut(s)	285, 726
BfaI	CTAG	3 cut(s)	62, 158, 527
BfmI	CTRYAG	1 cut(s)	562
BfoI	RGCGCY	1 cut(s)	225
BfuAI	ACCTGC	1 cut(s)	813
BfuI	GTATCC	1 cut(s)	761
BisI	GCNGC	5 cut(s)	177, 186, 405, 465, 555
BlsI	GCNGC	5 cut(s)	178, 187, 406, 466, 556
Bme1390I	CCNGG	5 cut(s)	303, 369, 461, 507, 963
Bme18I	GGWCC	1 cut(s)	959
BmgT120I	GGNCC	1 cut(s)	959
BmiI	GGNNCC	5 cut(s)	300, 418, 799, 805, 1054
BmrFI	CCNGG	5 cut(s)	303, 369, 461, 507, 963
BoxI	GACNNNNGTC	1 cut(s)	964
BplI	GAGNNNNNCTC	2 cut(s)	418, 450
BpmI	CTGGAG	1 cut(s)	916
BpuEI	CTTGAG	1 cut(s)	720
BsaBI	GATNNNNATC	1 cut(s)	75
BsaJI	CCNNGG	5 cut(s)	21, 394, 577, 913, 962
BsaWI	WCCGGW	2 cut(s)	800, 806
Bsc4I	CCNNNNNNNGG	2 cut(s)	148, 506
Bse118I	RCCGGY	1 cut(s)	52
Bse1I	ACTGG	1 cut(s)	799
Bse3DI	GCAATG	3 cut(s)	116, 353, 537
Bse8I	GATNNNNATC	1 cut(s)	75
BseBI	CCWGG	5 cut(s)	303, 369, 461, 507, 963
BseDI	CCNNGG	5 cut(s)	21, 394, 577, 913, 962
BseGI	GGATG	1 cut(s)	837
BseJI	GATNNNNATC	1 cut(s)	75
BseLI	CCNNNNNNNGG	2 cut(s)	148, 506
BseMI	GCAATG	3 cut(s)	116, 353, 537
BseMII	CTCAG	1 cut(s)	422
BseNI	ACTGG	1 cut(s)	799
BseRI	GAGGAG	2 cut(s)	684, 1062
BseSI	GKGCMC	2 cut(s)	155, 783
BseXI	GCAGC	4 cut(s)	163, 197, 476, 566
Bsh1236I	CGCG	2 cut(s)	190, 915
BsiHKAI	GWGCWC	1 cut(s)	783
BsiSI	CCGG	3 cut(s)	53, 801, 807
BslFI	GGGAC	2 cut(s)	412, 531
BslI	CCNNNNNNNGG	2 cut(s)	148, 506
BsmFI	GGGAC	2 cut(s)	412, 531
BsmI	GAATGC	2 cut(s)	29, 258
Bsp1286I	GDGCHC	4 cut(s)	100, 155, 783, 1055
Bsp143I	GATC	4 cut(s)	76, 285, 537, 726
Bsp19I	CCATGG	1 cut(s)	577
BspACI	CCGC	6 cut(s)	107, 165, 374, 405, 913, 915
BspCNI	CTCAG	1 cut(s)	421
BspFNI	CGCG	2 cut(s)	190, 915
BspLI	GGNNCC	5 cut(s)	300, 418, 799, 805, 1054
BspMI	ACCTGC	1 cut(s)	813
BspPI	GGATC	2 cut(s)	71, 545
BsrBI	CCGCTC	1 cut(s)	407
BsrDI	GCAATG	3 cut(s)	116, 353, 537
BsrFI	RCCGGY	1 cut(s)	52
BsrI	ACTGG	1 cut(s)	799
BssAI	RCCGGY	1 cut(s)	52
BssECI	CCNNGG	5 cut(s)	21, 394, 577, 913, 962
BssMI	GATC	4 cut(s)	76, 285, 537, 726
BssNAI	GTATAC	1 cut(s)	310
BssT1I	CCWWGG	1 cut(s)	577
Bst1107I	GTATAC	1 cut(s)	310
Bst2UI	CCWGG	5 cut(s)	303, 369, 461, 507, 963
Bst4CI	ACNGT	5 cut(s)	205, 281, 382, 421, 931
Bst6I	CTCTTC	2 cut(s)	661, 974
BstC8I	GCNNGC	2 cut(s)	155, 702
BstDEI	CTNAG	2 cut(s)	408, 973
BstDSI	CCRYGG	4 cut(s)	21, 394, 577, 913
BstF5I	GGATG	1 cut(s)	837
BstFNI	CGCG	2 cut(s)	190, 915
BstH2I	RGCGCY	1 cut(s)	225
BstHHI	GCGC	2 cut(s)	190, 224
BstKTI	GATC	4 cut(s)	79, 288, 540, 729
BstMBI	GATC	4 cut(s)	76, 285, 537, 726
BstMWI	GCNNNNNNNGC	4 cut(s)	173, 182, 185, 551
BstNI	CCWGG	5 cut(s)	303, 369, 461, 507, 963
BstPAI	GACNNNNGTC	1 cut(s)	964
BstSCI	CCNGG	5 cut(s)	301, 367, 459, 505, 961
BstSFI	CTRYAG	1 cut(s)	562
BstSLI	GKGCMC	2 cut(s)	155, 783
BstUI	CGCG	2 cut(s)	190, 915
BstV1I	GCAGC	4 cut(s)	163, 197, 476, 566
BstX2I	RGATCY	1 cut(s)	76
BstXI	CCANNNNNNTGG	1 cut(s)	894
BstYI	RGATCY	1 cut(s)	76
BstZ17I	GTATAC	1 cut(s)	310
BsuI	GTATCC	1 cut(s)	761
BtgI	CCRYGG	4 cut(s)	21, 394, 577, 913
BtsCI	GGATG	1 cut(s)	837
BtsI	GCAGTG	2 cut(s)	486, 781
BtsIMutI	CAGTG	3 cut(s)	486, 781, 792
BveI	ACCTGC	1 cut(s)	813
Cac8I	GCNNGC	2 cut(s)	155, 702
CfoI	GCGC	2 cut(s)	190, 224
Cfr10I	RCCGGY	1 cut(s)	52
Cfr13I	GGNCC	1 cut(s)	959
Cfr42I	CCGCGG	1 cut(s)	916
CsiI	ACCWGGT	2 cut(s)	367, 505
Csp6I	GTAC	2 cut(s)	412, 682
CspCI	CAANNNNNGTGG	1 cut(s)	38
CviAII	CATG	2 cut(s)	578, 1059
CviQI	GTAC	2 cut(s)	412, 682
DdeI	CTNAG	2 cut(s)	408, 973
DpnI	GATC	4 cut(s)	78, 287, 539, 728
DpnII	GATC	4 cut(s)	76, 285, 537, 726
DraI	TTTAAA	1 cut(s)	118
Eam1104I	CTCTTC	2 cut(s)	661, 974
EarI	CTCTTC	2 cut(s)	661, 974
Eco130I	CCWWGG	1 cut(s)	577
Eco24I	GRGCYC	2 cut(s)	100, 1055
Eco47I	GGWCC	1 cut(s)	959
Eco57I	CTGAAG	1 cut(s)	201
EcoRII	CCWGG	5 cut(s)	301, 367, 459, 505, 961
EcoT14I	CCWWGG	1 cut(s)	577
EcoT38I	GRGCYC	2 cut(s)	100, 1055
ErhI	CCWWGG	1 cut(s)	577
FaeI	CATG	2 cut(s)	581, 1062
FalI	AAGNNNNNCTT	2 cut(s)	658, 690
FaqI	GGGAC	2 cut(s)	412, 531
FatI	CATG	2 cut(s)	577, 1058
FauI	CCCGC	2 cut(s)	114, 172
FbaI	TGATCA	2 cut(s)	285, 726
FblI	GTMKAC	2 cut(s)	309, 742
Fnu4HI	GCNGC	5 cut(s)	177, 186, 405, 465, 555
FokI	GGATG	1 cut(s)	844
FriOI	GRGCYC	2 cut(s)	100, 1055
Fsp4HI	GCNGC	5 cut(s)	177, 186, 405, 465, 555
FspBI	CTAG	3 cut(s)	62, 158, 527
GlaI	GCGC	2 cut(s)	189, 223
GluI	GCNGC	5 cut(s)	177, 186, 405, 465, 555
GsuI	CTGGAG	1 cut(s)	916
HaeII	RGCGCY	1 cut(s)	225
HapII	CCGG	3 cut(s)	53, 801, 807
HhaI	GCGC	2 cut(s)	190, 224
Hin1II	CATG	2 cut(s)	581, 1062
Hin6I	GCGC	2 cut(s)	188, 222
HinP1I	GCGC	2 cut(s)	188, 222
HincII	GTYRAC	1 cut(s)	365
HindII	GTYRAC	1 cut(s)	365
HinfI	GANTC	4 cut(s)	82, 208, 573, 810
HpaII	CCGG	3 cut(s)	53, 801, 807
HphI	GGTGA	3 cut(s)	193, 461, 466
Hpy166II	GTNNAC	5 cut(s)	310, 365, 512, 743, 781
Hpy188I	TCNGA	3 cut(s)	81, 264, 959
Hpy188III	TCNNGA	5 cut(s)	86, 232, 253, 385, 587
Hpy8I	GTNNAC	5 cut(s)	310, 365, 512, 743, 781
HpyAV	CCTTC	2 cut(s)	313, 635
HpyCH4III	ACNGT	5 cut(s)	205, 281, 382, 421, 931
HpyCH4IV	ACGT	2 cut(s)	126, 773
HpyCH4V	TGCA	6 cut(s)	29, 431, 700, 752, 781, 786
HpyF10VI	GCNNNNNNNGC	4 cut(s)	173, 182, 185, 551
HpyF3I	CTNAG	2 cut(s)	408, 973
HpySE526I	ACGT	2 cut(s)	126, 773
Hsp92II	CATG	2 cut(s)	581, 1062
HspAI	GCGC	2 cut(s)	188, 222
Ksp22I	TGATCA	2 cut(s)	285, 726
KspI	CCGCGG	1 cut(s)	916
Kzo9I	GATC	4 cut(s)	76, 285, 537, 726
LmnI	GCTCC	4 cut(s)	304, 496, 671, 1058
Lsp1109I	GCAGC	4 cut(s)	163, 197, 476, 566
MabI	ACCWGGT	2 cut(s)	367, 505
MaeI	CTAG	3 cut(s)	62, 158, 527
MaeII	ACGT	2 cut(s)	126, 773
MaeIII	GTNAC	8 cut(s)	5, 322, 599, 632, 790, 966, 1018, 1040
MalI	GATC	4 cut(s)	78, 287, 539, 728
MbiI	CCGCTC	1 cut(s)	407
MboI	GATC	4 cut(s)	76, 285, 537, 726
MboII	GAAGA	3 cut(s)	613, 678, 991
MfeI	CAATTG	1 cut(s)	695
MflI	RGATCY	1 cut(s)	76
MhlI	GDGCHC	4 cut(s)	100, 155, 783, 1055
MluCI	AATT	3 cut(s)	195, 226, 695
MmeI	TCCRAC	2 cut(s)	287, 688
MnlI	CCTC	9 cut(s)	179, 482, 528, 662, 891, 917, 1040, 1080, 1083
MseI	TTAA	6 cut(s)	117, 129, 239, 480, 719, 1047
MslI	CAYNNNNRTG	3 cut(s)	14, 776, 866
MspA1I	CMGCKG	1 cut(s)	915
MspI	CCGG	3 cut(s)	53, 801, 807
MspR9I	CCNGG	5 cut(s)	303, 369, 461, 507, 963
MunI	CAATTG	1 cut(s)	695
Mva1269I	GAATGC	2 cut(s)	29, 258
MvaI	CCWGG	5 cut(s)	303, 369, 461, 507, 963
MvnI	CGCG	2 cut(s)	190, 915
MwoI	GCNNNNNNNGC	4 cut(s)	173, 182, 185, 551
NcoI	CCATGG	1 cut(s)	577
NdeII	GATC	4 cut(s)	76, 285, 537, 726
NlaIII	CATG	2 cut(s)	581, 1062
NlaIV	GGNNCC	5 cut(s)	300, 418, 799, 805, 1054
NmuCI	GTSAC	4 cut(s)	322, 599, 790, 966
OliI	CACNNNNGTG	1 cut(s)	776
PctI	GAATGC	2 cut(s)	29, 258
PfeI	GAWTC	4 cut(s)	82, 208, 573, 810
PkrI	GCNGC	5 cut(s)	178, 187, 406, 466, 556
PshAI	GACNNNNGTC	1 cut(s)	964
Psp1406I	AACGTT	1 cut(s)	126
Psp6I	CCWGG	5 cut(s)	301, 367, 459, 505, 961
PspGI	CCWGG	5 cut(s)	301, 367, 459, 505, 961
PspN4I	GGNNCC	5 cut(s)	300, 418, 799, 805, 1054
PspPI	GGNCC	1 cut(s)	959
PsuI	RGATCY	1 cut(s)	76
RsaI	GTAC	2 cut(s)	413, 683
RsaNI	GTAC	2 cut(s)	412, 682
RseI	CAYNNNNRTG	3 cut(s)	14, 776, 866
SacII	CCGCGG	1 cut(s)	916
SaqAI	TTAA	6 cut(s)	117, 129, 239, 480, 719, 1047
SatI	GCNGC	5 cut(s)	177, 186, 405, 465, 555
Sau3AI	GATC	4 cut(s)	76, 285, 537, 726
Sau96I	GGNCC	1 cut(s)	959
ScrFI	CCNGG	5 cut(s)	303, 369, 461, 507, 963
SduI	GDGCHC	4 cut(s)	100, 155, 783, 1055
SexAI	ACCWGGT	2 cut(s)	367, 505
SfcI	CTRYAG	1 cut(s)	562
Sfr303I	CCGCGG	1 cut(s)	916
SgrAI	CRCCGGYG	1 cut(s)	52
SgrBI	CCGCGG	1 cut(s)	916
SinI	GGWCC	1 cut(s)	959
SmiMI	CAYNNNNRTG	3 cut(s)	14, 776, 866
SmlI	CTYRAG	1 cut(s)	735
SmoI	CTYRAG	1 cut(s)	735
Sse9I	AATT	3 cut(s)	195, 226, 695
SsiI	CCGC	6 cut(s)	107, 165, 374, 405, 913, 915
SspI	AATATT	1 cut(s)	455
SspMI	CTAG	3 cut(s)	62, 158, 527
StyD4I	CCNGG	5 cut(s)	301, 367, 459, 505, 961
StyI	CCWWGG	1 cut(s)	577
TaaI	ACNGT	5 cut(s)	205, 281, 382, 421, 931
TaiI	ACGT	2 cut(s)	129, 776
TaqI	TCGA	4 cut(s)	171, 330, 340, 639
TasI	AATT	3 cut(s)	195, 226, 695
TatI	WGTACW	1 cut(s)	681
TauI	GCSGC	1 cut(s)	407
TfiI	GAWTC	4 cut(s)	82, 208, 573, 810
Tru1I	TTAA	6 cut(s)	117, 129, 239, 480, 719, 1047
Tru9I	TTAA	6 cut(s)	117, 129, 239, 480, 719, 1047
TscAI	CASTG	3 cut(s)	493, 788, 799
TseFI	GTSAC	4 cut(s)	322, 599, 790, 966
TseI	GCWGC	4 cut(s)	176, 185, 464, 554
Tsp45I	GTSAC	4 cut(s)	322, 599, 790, 966
TspDTI	ATGAA	1 cut(s)	337
TspGWI	ACGGA	3 cut(s)	10, 429, 780
TspRI	CASTG	3 cut(s)	493, 788, 799
VneI	GTGCAC	1 cut(s)	779
VpaK11BI	GGWCC	1 cut(s)	959
XmiI	GTMKAC	2 cut(s)	309, 742
XspI	CTAG	3 cut(s)	62, 158, 527

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.