FvH4_1g09290

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001024
Physical Location & Seq
Reverse (-)
4968539 .. 4971093
2555 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g09290.t1

Sequence Viewer

Length: 1122 bp
ATGGCTTCGAAACCGACCTCGGCTGTTTTGGTCGTGTCGGCATTTCTGACTTTGGCTTTGCTCGTGTCCGGCAAACCTAACCTCATCCCGGAGAACGAGGCCGATCTTGATGACTGGATCGTCCGAAACATGAGGGAGTACGACAAGCGGAAGGCCGTGCTCAATACGAGGAGTGACGTCCCCGGCACGCACTTTGACACGGAGCTTGCGGCGGCTGAGGACGCAGGGAGAGTCATAAGGGTGAAGAAGGACGGCACTGGAGACTTTAAGACGGTGACGGACGCCGTGAAGAGCATTCCTACGGGGAACAAGCGGCGCGTGGTGGTGTGGATTGGTGGGGGAGAGTATAGAGAGAAGATTCTGGTGGAGATGACGAAACCCTTCGTGACGTTTTATGGGGATAAGAATGATATGCCGTCGATCACGTACGACGGCACTGCGGCGGTGTATGGGACGTGGAACAGTGCCACAGTGGCGGTGGAGTCCGACTATTTCGTTGCCGTGAATATTGCGTTTGTGAACTCAGCTCCTATGCCAGACGGTATAAGGAAGGATGCACAGGCGGTGGCAATGAGAATATCAGGGGACAAGGCTGCATTCTACAACTGCAAGTTCATGGGATTTCAGGACACCTTATGTGACGATAAGGGAAGACATTTCTTCAAGGATTGCTTCATCCAAGGCACTGTTGATTTCATCTTTGGCAATGGAAAATCCCTCTACTTGAAAAGTACCCTAAACTCAGTCGCGAATGGAGTTGGGGTGATCACAGCACACGGTAGATCAGAAGCGCAGGACGATACTGGATTTTCGTTCGTGTATTGCACCATAAAAGGGACAGGTGACACATTGCTAGGTCGTGCTTGGAGGGGAAGAGCAAGAGTTGTGTTTGCCTACACATACATGGGGAACCTTGTCGACAGAAAAGGGTGGTCCGACTACAACGACAATACGCGTGCCAGCACTGTGTATTATGGAGAGTACAAGTGTTCTGGACCGGGTTCAAGCTCCAACGAACGAGTCAAGTATGCAAAGATGCTGAGCGACGTAGAAGCAAGACCCTTCATAAGCTTGACCTTCATCCAAGGAACGAAATGGGTTCTTCCCCCTCCCAACCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

374

Amino Acids

41.09

Weight (kDa)

8.61

Isoelectric Point (pI)

21.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 80 - 363 1.3e-71 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AatII GACGTC 1 cut(s) 180
AccI GTMKAC 1 cut(s) 918
AccII CGCG 3 cut(s) 318, 749, 955
AciI CCGC 8 cut(s) 148, 209, 212, 313, 440, 443, 476, 563
AclWI GGATC 1 cut(s) 125
AcyI GRCGYC 2 cut(s) 177, 282
AfaI GTAC 4 cut(s) 140, 428, 733, 983
AfiI CCNNNNNNNGG 2 cut(s) 88, 834
AflIII ACRYGT 1 cut(s) 953
AgsI TTSAA 3 cut(s) 664, 727, 1005
AjiI CACGTC 1 cut(s) 456
AluBI AGCT 4 cut(s) 205, 527, 1008, 1071
AluI AGCT 4 cut(s) 205, 527, 1008, 1071
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 1 cut(s) 255
AlwI GGATC 1 cut(s) 125
AoxI GGCC 2 cut(s) 99, 153
ApeKI GCWGC 1 cut(s) 593
ArsI GACNNNNNNTTYG 2 cut(s) 40, 72
Asp700I GAANNNNTTC 1 cut(s) 380
AspLEI GCGC 2 cut(s) 318, 793
AspS9I GGNCC 2 cut(s) 933, 995
AsuC2I CCSGG 3 cut(s) 89, 183, 999
AsuHPI GGTGA 4 cut(s) 253, 286, 775, 854
AsuII TTCGAA 1 cut(s) 8
AvaII GGWCC 2 cut(s) 933, 995
BauI CACGAG 1 cut(s) 62
BbsI GAAGAC 1 cut(s) 658
Bbv12I GWGCWC 1 cut(s) 162
BbvCI CCTCAGC 1 cut(s) 216
BbvI GCAGC 1 cut(s) 580
BceAI ACGGC 6 cut(s) 140, 268, 269, 400, 448, 485
BcgI CGANNNNNNTGC 2 cut(s) 419, 453
BclI TGATCA 1 cut(s) 765
BcnI CCSGG 3 cut(s) 89, 183, 999
BcoDI GTCTC 1 cut(s) 255
BfaI CTAG 2 cut(s) 854, 1120
BglI GCCNNNNNGGC 1 cut(s) 473
BisI GCNGC 5 cut(s) 210, 213, 314, 441, 594
BlpI GCTNAGC 1 cut(s) 1040
BlsI GCNGC 5 cut(s) 211, 214, 315, 442, 595
Bme1390I CCNGG 3 cut(s) 89, 183, 999
Bme18I GGWCC 2 cut(s) 933, 995
BmgBI CACGTC 1 cut(s) 456
BmgT120I GGNCC 2 cut(s) 933, 995
BmiI GGNNCC 1 cut(s) 911
BmrFI CCNGG 3 cut(s) 89, 183, 999
BmsI GCATC 2 cut(s) 544, 1026
BpiI GAAGAC 1 cut(s) 658
BpmI CTGGAG 1 cut(s) 279
Bpu10I CCTNAGC 1 cut(s) 216
Bpu1102I GCTNAGC 1 cut(s) 1040
Bpu14I TTCGAA 1 cut(s) 8
BpuMI CCSGG 3 cut(s) 89, 183, 999
BsaAI YACGTR 1 cut(s) 426
BsaHI GRCGYC 2 cut(s) 177, 282
BsaJI CCNNGG 4 cut(s) 18, 181, 679, 1084
BsaXI ACNNNNNCTCC 2 cut(s) 747, 777
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 834
Bse1I ACTGG 3 cut(s) 119, 262, 808
Bse3DI GCAATG 3 cut(s) 576, 712, 848
BseDI CCNNGG 4 cut(s) 18, 181, 679, 1084
BseGI GGATG 4 cut(s) 84, 559, 675, 1080
BseLI CCNNNNNNNGG 2 cut(s) 88, 834
BseMI GCAATG 3 cut(s) 576, 712, 848
BseMII CTCAG 4 cut(s) 207, 537, 756, 1031
BseNI ACTGG 3 cut(s) 119, 262, 808
BseRI GAGGAG 1 cut(s) 184
BseXI GCAGC 1 cut(s) 580
Bsh1236I CGCG 3 cut(s) 318, 749, 955
BshFI GGCC 2 cut(s) 101, 155
BsiHKAI GWGCWC 1 cut(s) 162
BsiSI CCGG 4 cut(s) 69, 89, 183, 998
BsiWI CGTACG 1 cut(s) 426
BslFI GGGAC 4 cut(s) 164, 466, 599, 850
BslI CCNNNNNNNGG 2 cut(s) 88, 834
BsmAI GTCTC 1 cut(s) 255
BsmFI GGGAC 4 cut(s) 164, 466, 599, 850
BsmI GAATGC 2 cut(s) 294, 596
BsnI GGCC 2 cut(s) 101, 155
Bsp119I TTCGAA 1 cut(s) 8
Bsp1286I GDGCHC 1 cut(s) 162
Bsp143I GATC 5 cut(s) 103, 117, 420, 765, 782
Bsp1720I GCTNAGC 1 cut(s) 1040
Bsp68I TCGCGA 1 cut(s) 749
BspACI CCGC 8 cut(s) 148, 209, 212, 313, 440, 443, 476, 563
BspANI GGCC 2 cut(s) 101, 155
BspCNI CTCAG 4 cut(s) 208, 536, 755, 1032
BspFNI CGCG 3 cut(s) 318, 749, 955
BspLI GGNNCC 1 cut(s) 911
BspPI GGATC 1 cut(s) 125
BspQI GCTCTTC 2 cut(s) 284, 868
BspT104I TTCGAA 1 cut(s) 8
BsrDI GCAATG 3 cut(s) 576, 712, 848
BsrI ACTGG 3 cut(s) 119, 262, 808
BssECI CCNNGG 4 cut(s) 18, 181, 679, 1084
BssMI GATC 5 cut(s) 103, 117, 420, 765, 782
BssNI GRCGYC 2 cut(s) 177, 282
BssSI CACGAG 1 cut(s) 62
BssT1I CCWWGG 2 cut(s) 679, 1084
Bst2BI CACGAG 1 cut(s) 62
Bst4CI ACNGT 7 cut(s) 274, 464, 472, 542, 688, 779, 967
Bst6I CTCTTC 2 cut(s) 284, 868
BstACI GRCGYC 2 cut(s) 177, 282
BstBAI YACGTR 1 cut(s) 426
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 4 cut(s) 188, 207, 957, 961
BstDEI CTNAG 4 cut(s) 216, 523, 742, 1040
BstF5I GGATG 4 cut(s) 84, 559, 675, 1080
BstFNI CGCG 3 cut(s) 318, 749, 955
BstHHI GCGC 2 cut(s) 318, 793
BstKTI GATC 5 cut(s) 106, 120, 423, 768, 785
BstMAI GTCTC 1 cut(s) 255
BstMBI GATC 5 cut(s) 103, 117, 420, 765, 782
BstMWI GCNNNNNNNGC 2 cut(s) 221, 473
BstSCI CCNGG 3 cut(s) 87, 181, 997
BstUI CGCG 3 cut(s) 318, 749, 955
BstV1I GCAGC 1 cut(s) 580
BstV2I GAAGAC 1 cut(s) 658
BsuRI GGCC 2 cut(s) 101, 155
BtrI CACGTC 1 cut(s) 456
BtsCI GGATG 4 cut(s) 84, 559, 675, 1080
BtsI GCAGTG 1 cut(s) 435
BtsIMutI CAGTG 6 cut(s) 255, 435, 469, 477, 684, 963
BtuMI TCGCGA 1 cut(s) 749
Cac8I GCNNGC 4 cut(s) 188, 207, 957, 961
CfoI GCGC 2 cut(s) 318, 793
Cfr13I GGNCC 2 cut(s) 933, 995
CseI GACGC 2 cut(s) 230, 290
Csp6I GTAC 4 cut(s) 139, 427, 732, 982
CviAII CATG 3 cut(s) 130, 616, 904
CviQI GTAC 4 cut(s) 139, 427, 732, 982
DdeI CTNAG 4 cut(s) 216, 523, 742, 1040
DpnI GATC 5 cut(s) 105, 119, 422, 767, 784
DpnII GATC 5 cut(s) 103, 117, 420, 765, 782
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
Eam1104I CTCTTC 2 cut(s) 284, 868
EarI CTCTTC 2 cut(s) 284, 868
Eco130I CCWWGG 2 cut(s) 679, 1084
Eco47I GGWCC 2 cut(s) 933, 995
EcoT14I CCWWGG 2 cut(s) 679, 1084
ErhI CCWWGG 2 cut(s) 679, 1084
FaeI CATG 3 cut(s) 133, 619, 907
FalI AAGNNNNNCTT 2 cut(s) 656, 688
FaqI GGGAC 4 cut(s) 164, 466, 599, 850
FatI CATG 3 cut(s) 129, 615, 903
FbaI TGATCA 1 cut(s) 765
FblI GTMKAC 1 cut(s) 918
Fnu4HI GCNGC 5 cut(s) 210, 213, 314, 441, 594
FokI GGATG 4 cut(s) 71, 566, 662, 1067
Fsp4HI GCNGC 5 cut(s) 210, 213, 314, 441, 594
FspBI CTAG 2 cut(s) 854, 1120
GlaI GCGC 2 cut(s) 317, 792
GluI GCNGC 5 cut(s) 210, 213, 314, 441, 594
GsuI CTGGAG 1 cut(s) 279
HaeIII GGCC 2 cut(s) 101, 155
HapII CCGG 4 cut(s) 69, 89, 183, 998
HgaI GACGC 2 cut(s) 230, 290
HhaI GCGC 2 cut(s) 318, 793
Hin1I GRCGYC 2 cut(s) 177, 282
Hin1II CATG 3 cut(s) 133, 619, 907
Hin6I GCGC 2 cut(s) 316, 791
HinP1I GCGC 2 cut(s) 316, 791
HincII GTYRAC 1 cut(s) 919
HindII GTYRAC 1 cut(s) 919
HindIII AAGCTT 1 cut(s) 1069
HinfI GANTC 4 cut(s) 231, 358, 482, 1020
HpaII CCGG 4 cut(s) 69, 89, 183, 998
HphI GGTGA 4 cut(s) 253, 286, 775, 854
Hpy166II GTNNAC 2 cut(s) 520, 919
Hpy188I TCNGA 5 cut(s) 48, 125, 487, 787, 937
Hpy188III TCNNGA 5 cut(s) 107, 385, 626, 748, 993
Hpy8I GTNNAC 2 cut(s) 520, 919
Hpy99I CGWCG 3 cut(s) 421, 434, 1049
HpyAV CCTTC 6 cut(s) 145, 241, 391, 544, 1072, 1087
HpyCH4III ACNGT 7 cut(s) 274, 464, 472, 542, 688, 779, 967
HpyCH4IV ACGT 5 cut(s) 177, 389, 425, 455, 1047
HpyCH4V TGCA 5 cut(s) 557, 596, 609, 825, 1031
HpyF10VI GCNNNNNNNGC 2 cut(s) 221, 473
HpyF3I CTNAG 4 cut(s) 216, 523, 742, 1040
HpySE526I ACGT 5 cut(s) 177, 389, 425, 455, 1047
Hsp92I GRCGYC 2 cut(s) 177, 282
Hsp92II CATG 3 cut(s) 133, 619, 907
HspAI GCGC 2 cut(s) 316, 791
Ksp22I TGATCA 1 cut(s) 765
Kzo9I GATC 5 cut(s) 103, 117, 420, 765, 782
LguI GCTCTTC 2 cut(s) 284, 868
LmnI GCTCC 3 cut(s) 202, 532, 1013
Lsp1109I GCAGC 1 cut(s) 580
LweI GCATC 2 cut(s) 544, 1026
MaeI CTAG 2 cut(s) 854, 1120
MaeII ACGT 5 cut(s) 177, 389, 425, 455, 1047
MaeIII GTNAC 5 cut(s) 173, 274, 385, 638, 842
MalI GATC 5 cut(s) 105, 119, 422, 767, 784
MboI GATC 5 cut(s) 103, 117, 420, 765, 782
MboII GAAGA 7 cut(s) 256, 301, 367, 652, 663, 885, 1094
MhlI GDGCHC 1 cut(s) 162
MluI ACGCGT 1 cut(s) 953
MlyI GAGTC 3 cut(s) 240, 491, 1029
MmeI TCCRAC 3 cut(s) 510, 960, 1035
MnlI CCTC 9 cut(s) 28, 91, 92, 126, 162, 211, 728, 861, 1119
MroXI GAANNNNTTC 1 cut(s) 380
MseI TTAA 1 cut(s) 267
MslI CAYNNNNRTG 2 cut(s) 239, 902
MspI CCGG 4 cut(s) 69, 89, 183, 998
MspR9I CCNGG 3 cut(s) 89, 183, 999
Mva1269I GAATGC 2 cut(s) 294, 596
MvnI CGCG 3 cut(s) 318, 749, 955
MwoI GCNNNNNNNGC 2 cut(s) 221, 473
NciI CCSGG 3 cut(s) 89, 183, 999
NdeII GATC 5 cut(s) 103, 117, 420, 765, 782
NlaIII CATG 3 cut(s) 133, 619, 907
NlaIV GGNNCC 1 cut(s) 911
NmuCI GTSAC 5 cut(s) 173, 274, 385, 638, 842
NruI TCGCGA 1 cut(s) 749
NspV TTCGAA 1 cut(s) 8
PciSI GCTCTTC 2 cut(s) 284, 868
PctI GAATGC 2 cut(s) 294, 596
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 1 cut(s) 358
Pfl23II CGTACG 1 cut(s) 426
PfoI TCCNGGA 1 cut(s) 87
PkrI GCNGC 5 cut(s) 211, 214, 315, 442, 595
PleI GAGTC 3 cut(s) 239, 490, 1028
PpsI GAGTC 3 cut(s) 239, 490, 1028
Ppu21I YACGTR 1 cut(s) 426
PspLI CGTACG 1 cut(s) 426
PspN4I GGNNCC 1 cut(s) 911
PspPI GGNCC 2 cut(s) 933, 995
RruI TCGCGA 1 cut(s) 749
RsaI GTAC 4 cut(s) 140, 428, 733, 983
RsaNI GTAC 4 cut(s) 139, 427, 732, 982
RseI CAYNNNNRTG 2 cut(s) 239, 902
SalI GTCGAC 1 cut(s) 917
SapI GCTCTTC 2 cut(s) 284, 868
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 5 cut(s) 210, 213, 314, 441, 594
Sau3AI GATC 5 cut(s) 103, 117, 420, 765, 782
Sau96I GGNCC 2 cut(s) 933, 995
SchI GAGTC 3 cut(s) 240, 491, 1029
ScrFI CCNGG 3 cut(s) 89, 183, 999
SduI GDGCHC 1 cut(s) 162
SfaNI GCATC 2 cut(s) 544, 1026
SfuI TTCGAA 1 cut(s) 8
SinI GGWCC 2 cut(s) 933, 995
SmiMI CAYNNNNRTG 2 cut(s) 239, 902
SsiI CCGC 8 cut(s) 148, 209, 212, 313, 440, 443, 476, 563
SspI AATATT 1 cut(s) 508
SspMI CTAG 2 cut(s) 854, 1120
StyD4I CCNGG 3 cut(s) 87, 181, 997
StyI CCWWGG 2 cut(s) 679, 1084
TaaI ACNGT 7 cut(s) 274, 464, 472, 542, 688, 779, 967
TaiI ACGT 5 cut(s) 180, 392, 428, 458, 1050
TaqI TCGA 3 cut(s) 8, 419, 918
TatI WGTACW 1 cut(s) 981
TauI GCSGC 4 cut(s) 212, 215, 316, 443
TfiI GAWTC 1 cut(s) 358
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 6 cut(s) 262, 442, 469, 477, 691, 970
TseFI GTSAC 5 cut(s) 173, 274, 385, 638, 842
TseI GCWGC 1 cut(s) 593
Tsp45I GTSAC 5 cut(s) 173, 274, 385, 638, 842
TspDTI ATGAA 5 cut(s) 604, 664, 685, 1054, 1069
TspGWI ACGGA 2 cut(s) 215, 293
TspRI CASTG 6 cut(s) 262, 442, 469, 477, 691, 970
VpaK11BI GGWCC 2 cut(s) 933, 995
XcmI CCANNNNNNNNNTGG 1 cut(s) 475
XmiI GTMKAC 1 cut(s) 918
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 2 cut(s) 854, 1120
ZraI GACGTC 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.