Rh2AG102400

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001024
Physical Location & Seq
Reverse (-)
8717590 .. 8721323
3734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG102400.1

Sequence Viewer

Length: 813 bp
ATGGCTATGAGATCGACACCCGCTCTTGTGGTCGTTGCGGCATTTCTGGCTCTCCATTTCGTGCCTTTGCTCGTGTCGGGCCACCCCAACCTCATTCCGGAAAACGAGTCTGATCTCGATGGCTGGATCGTCCAAAACATGAGGGAGTACAACGAGCGGAAGGCCGAGCTGAGGACCAGGAGCAACGTCCCCGGCACTCACTTCGACACAGGGCTCGCCTCCGCCGAGGACGCAAAGAGAGTCATCCGGGTGAAGCAGGACGGCACCGGAGACTTTAGGACGGTGATGGACGCCGTGAGGAGCATTCCGTCTGCAAACAAGCAGCGCGTGGTGGTGTGGATTGGCGGGGGAGAGTACAAGGAGAAGGTTCTGGTGGACGTGACGAAGCCGTACGTGACATTTTTTGGGGACAAGAACAACATCCCGTCGATCACCTACGACGGCACGGCTGCCCTGTACGGGACGTGGAACAGTGCCACGGTGGCTGTGGAAGCCGACTATTTCATGGCCGTGAATATAGCGTTCGTGAATTCAGCTCCTATGCCGGACGGCATAAGGAAAGGTGCACAGGCGGTGGCAATGAGAATATCAGGGGATAAGGCAGCCTTCCACAACTGCAGGTTCATCGGATTTCAGGACACCTTATGTGACGATAGGGGAAGGCATTTATTCAAGGATTGCTTCATCCAAGGCACTGTTGACTTCATCTTTGGCAATGGAAAATCCCTCTACTTGGTACACACATACCTATACTTGTTATGTCTTGTAATTGGGTTTTTAAATTTTATGTCCCTGAAATTTTTAGCTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

270

Amino Acids

29.93

Weight (kDa)

7.69

Isoelectric Point (pI)

25.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 83 - 243 5.2e-42 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 609
AccB1I GGYRCC 1 cut(s) 263
AccBSI CCGCTC 2 cut(s) 23, 157
AccII CGCG 1 cut(s) 327
AccIII TCCGGA 1 cut(s) 97
AciI CCGC 6 cut(s) 21, 38, 157, 222, 345, 572
AclWI GGATC 1 cut(s) 134
AcoI YGGCCR 1 cut(s) 507
AcsI RAATTY 3 cut(s) 529, 781, 797
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 5 cut(s) 149, 356, 392, 458, 738
AfiI CCNNNNNNNGG 4 cut(s) 97, 171, 459, 733
AgsI TTSAA 1 cut(s) 673
AjiI CACGTC 2 cut(s) 379, 465
AjnI CCWGG 1 cut(s) 176
AluBI AGCT 3 cut(s) 169, 536, 806
AluI AGCT 3 cut(s) 169, 536, 806
Alw21I GWGCWC 1 cut(s) 568
Alw26I GTCTC 1 cut(s) 264
Alw44I GTGCAC 1 cut(s) 564
AlwI GGATC 1 cut(s) 134
Aor13HI TCCGGA 1 cut(s) 97
AoxI GGCC 3 cut(s) 79, 162, 507
ApaLI GTGCAC 1 cut(s) 564
ApeKI GCWGC 3 cut(s) 322, 449, 602
ApoI RAATTY 3 cut(s) 529, 781, 797
ArsI GACNNNNNNTTYG 2 cut(s) 692, 724
AspLEI GCGC 1 cut(s) 327
AspS9I GGNCC 2 cut(s) 79, 174
AsuC2I CCSGG 2 cut(s) 192, 248
AsuHPI GGTGA 3 cut(s) 262, 295, 424
AvaII GGWCC 1 cut(s) 174
BaeGI GKGCMC 1 cut(s) 568
BanI GGYRCC 1 cut(s) 263
BanII GRGCYC 1 cut(s) 216
BauI CACGAG 1 cut(s) 71
Bbv12I GWGCWC 1 cut(s) 568
BbvCI CCTCAGC 1 cut(s) 170
BbvI GCAGC 3 cut(s) 334, 436, 614
BccI CCATC 2 cut(s) 113, 280
BceAI ACGGC 7 cut(s) 277, 278, 373, 457, 462, 494, 565
BcgI CGANNNNNNTGC 4 cut(s) 184, 218, 607, 641
BciT130I CCWGG 1 cut(s) 178
BcnI CCSGG 2 cut(s) 192, 248
BcoDI GTCTC 1 cut(s) 264
BfmI CTRYAG 1 cut(s) 616
BfuAI ACCTGC 1 cut(s) 609
BglI GCCNNNNNGGC 1 cut(s) 482
BisI GCNGC 4 cut(s) 39, 323, 450, 603
BlsI GCNGC 4 cut(s) 40, 324, 451, 604
Bme1390I CCNGG 3 cut(s) 178, 192, 248
Bme18I GGWCC 1 cut(s) 174
BmgBI CACGTC 2 cut(s) 379, 465
BmgT120I GGNCC 2 cut(s) 79, 174
BmiI GGNNCC 1 cut(s) 265
BmrFI CCNGG 3 cut(s) 178, 192, 248
Bpu10I CCTNAGC 1 cut(s) 170
BpuMI CCSGG 2 cut(s) 192, 248
BsaAI YACGTR 1 cut(s) 394
BsaHI GRCGYC 1 cut(s) 291
BsaJI CCNNGG 4 cut(s) 190, 225, 477, 688
BsaWI WCCGGW 2 cut(s) 97, 266
Bsc4I CCNNNNNNNGG 4 cut(s) 97, 171, 459, 733
Bse3DI GCAATG 2 cut(s) 585, 721
BseAI TCCGGA 1 cut(s) 97
BseBI CCWGG 1 cut(s) 178
BseDI CCNNGG 4 cut(s) 190, 225, 477, 688
BseGI GGATG 3 cut(s) 243, 420, 684
BseLI CCNNNNNNNGG 4 cut(s) 97, 171, 459, 733
BseMI GCAATG 2 cut(s) 585, 721
BseMII CTCAG 1 cut(s) 161
BseRI GAGGAG 1 cut(s) 313
BseSI GKGCMC 1 cut(s) 568
BseXI GCAGC 3 cut(s) 334, 436, 614
Bsh1236I CGCG 1 cut(s) 327
BshFI GGCC 3 cut(s) 81, 164, 509
BshNI GGYRCC 1 cut(s) 263
BsiHKAI GWGCWC 1 cut(s) 568
BsiSI CCGG 5 cut(s) 98, 192, 247, 267, 545
BsiWI CGTACG 1 cut(s) 390
BslFI GGGAC 4 cut(s) 173, 422, 475, 775
BslI CCNNNNNNNGG 4 cut(s) 97, 171, 459, 733
BsmAI GTCTC 1 cut(s) 264
BsmFI GGGAC 4 cut(s) 173, 422, 475, 775
BsmI GAATGC 1 cut(s) 303
BsnI GGCC 3 cut(s) 81, 164, 509
Bsp1286I GDGCHC 2 cut(s) 216, 568
Bsp13I TCCGGA 1 cut(s) 97
Bsp143I GATC 4 cut(s) 11, 112, 126, 429
BspACI CCGC 6 cut(s) 21, 38, 157, 222, 345, 572
BspANI GGCC 3 cut(s) 81, 164, 509
BspCNI CTCAG 1 cut(s) 162
BspEI TCCGGA 1 cut(s) 97
BspFNI CGCG 1 cut(s) 327
BspLI GGNNCC 1 cut(s) 265
BspMAI CTGCAG 1 cut(s) 620
BspMI ACCTGC 1 cut(s) 609
BspPI GGATC 1 cut(s) 134
BspT107I GGYRCC 1 cut(s) 263
BsrBI CCGCTC 2 cut(s) 23, 157
BsrDI GCAATG 2 cut(s) 585, 721
BssECI CCNNGG 4 cut(s) 190, 225, 477, 688
BssMI GATC 4 cut(s) 11, 112, 126, 429
BssNI GRCGYC 1 cut(s) 291
BssSI CACGAG 1 cut(s) 71
BssT1I CCWWGG 1 cut(s) 688
Bst2BI CACGAG 1 cut(s) 71
Bst2UI CCWGG 1 cut(s) 178
Bst4CI ACNGT 4 cut(s) 283, 473, 481, 697
BstACI GRCGYC 1 cut(s) 291
BstBAI YACGTR 1 cut(s) 394
BstC8I GCNNGC 1 cut(s) 216
BstDEI CTNAG 1 cut(s) 170
BstDSI CCRYGG 1 cut(s) 477
BstF5I GGATG 3 cut(s) 243, 420, 684
BstFNI CGCG 1 cut(s) 327
BstHHI GCGC 1 cut(s) 327
BstKTI GATC 4 cut(s) 14, 115, 129, 432
BstMAI GTCTC 1 cut(s) 264
BstMBI GATC 4 cut(s) 11, 112, 126, 429
BstMWI GCNNNNNNNGC 4 cut(s) 47, 230, 482, 491
BstNI CCWGG 1 cut(s) 178
BstSCI CCNGG 3 cut(s) 176, 190, 246
BstSFI CTRYAG 1 cut(s) 616
BstSLI GKGCMC 1 cut(s) 568
BstUI CGCG 1 cut(s) 327
BstV1I GCAGC 3 cut(s) 334, 436, 614
BsuRI GGCC 3 cut(s) 81, 164, 509
BtgI CCRYGG 1 cut(s) 477
BtrI CACGTC 2 cut(s) 379, 465
BtsCI GGATG 3 cut(s) 243, 420, 684
BtsIMutI CAGTG 2 cut(s) 478, 693
BveI ACCTGC 1 cut(s) 609
Cac8I GCNNGC 1 cut(s) 216
CfoI GCGC 1 cut(s) 327
Cfr13I GGNCC 2 cut(s) 79, 174
CseI GACGC 2 cut(s) 239, 299
Csp6I GTAC 5 cut(s) 148, 355, 391, 457, 737
CviAII CATG 2 cut(s) 139, 505
CviQI GTAC 5 cut(s) 148, 355, 391, 457, 737
DdeI CTNAG 1 cut(s) 170
DpnI GATC 4 cut(s) 13, 114, 128, 431
DpnII GATC 4 cut(s) 11, 112, 126, 429
DraI TTTAAA 1 cut(s) 780
EaeI YGGCCR 1 cut(s) 507
EciI GGCGGA 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 688
Eco24I GRGCYC 1 cut(s) 216
Eco47I GGWCC 1 cut(s) 174
EcoRI GAATTC 1 cut(s) 529
EcoRII CCWGG 1 cut(s) 176
EcoT14I CCWWGG 1 cut(s) 688
EcoT38I GRGCYC 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 688
FaeI CATG 2 cut(s) 142, 508
FalI AAGNNNNNCTT 4 cut(s) 590, 622, 665, 697
FaqI GGGAC 4 cut(s) 173, 422, 475, 775
FatI CATG 2 cut(s) 138, 504
FauI CCCGC 2 cut(s) 28, 338
Fnu4HI GCNGC 4 cut(s) 39, 323, 450, 603
FokI GGATG 3 cut(s) 230, 407, 671
FriOI GRGCYC 1 cut(s) 216
Fsp4HI GCNGC 4 cut(s) 39, 323, 450, 603
GlaI GCGC 1 cut(s) 326
GluI GCNGC 4 cut(s) 39, 323, 450, 603
HaeIII GGCC 3 cut(s) 81, 164, 509
HapII CCGG 5 cut(s) 98, 192, 247, 267, 545
HgaI GACGC 2 cut(s) 239, 299
HhaI GCGC 1 cut(s) 327
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 2 cut(s) 142, 508
Hin6I GCGC 1 cut(s) 325
HinP1I GCGC 1 cut(s) 325
HincII GTYRAC 1 cut(s) 700
HindII GTYRAC 1 cut(s) 700
HinfI GANTC 2 cut(s) 107, 240
HpaII CCGG 5 cut(s) 98, 192, 247, 267, 545
HphI GGTGA 3 cut(s) 262, 295, 424
Hpy166II GTNNAC 4 cut(s) 376, 566, 700, 739
Hpy188I TCNGA 2 cut(s) 112, 629
Hpy188III TCNNGA 4 cut(s) 98, 116, 526, 635
Hpy8I GTNNAC 4 cut(s) 376, 566, 700, 739
Hpy99I CGWCG 2 cut(s) 430, 443
HpyAV CCTTC 4 cut(s) 154, 358, 616, 654
HpyCH4III ACNGT 4 cut(s) 283, 473, 481, 697
HpyCH4IV ACGT 4 cut(s) 186, 378, 393, 464
HpyCH4V TGCA 3 cut(s) 314, 566, 618
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 230, 482, 491
HpyF3I CTNAG 1 cut(s) 170
HpySE526I ACGT 4 cut(s) 186, 378, 393, 464
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 2 cut(s) 142, 508
HspAI GCGC 1 cut(s) 325
Kpn2I TCCGGA 1 cut(s) 97
Kzo9I GATC 4 cut(s) 11, 112, 126, 429
LmnI GCTCC 3 cut(s) 180, 300, 541
Lsp1109I GCAGC 3 cut(s) 334, 436, 614
MaeII ACGT 4 cut(s) 186, 378, 393, 464
MaeIII GTNAC 3 cut(s) 379, 394, 647
MalI GATC 4 cut(s) 13, 114, 128, 431
MbiI CCGCTC 2 cut(s) 23, 157
MboI GATC 4 cut(s) 11, 112, 126, 429
MhlI GDGCHC 2 cut(s) 216, 568
MluCI AATT 4 cut(s) 529, 768, 781, 797
MlyI GAGTC 2 cut(s) 116, 249
MnlI CCTC 7 cut(s) 101, 135, 165, 220, 229, 291, 737
MroI TCCGGA 1 cut(s) 97
MseI TTAA 1 cut(s) 779
MslI CAYNNNNRTG 2 cut(s) 248, 509
MspI CCGG 5 cut(s) 98, 192, 247, 267, 545
MspR9I CCNGG 3 cut(s) 178, 192, 248
Mva1269I GAATGC 1 cut(s) 303
MvaI CCWGG 1 cut(s) 178
MvnI CGCG 1 cut(s) 327
MwoI GCNNNNNNNGC 4 cut(s) 47, 230, 482, 491
NciI CCSGG 2 cut(s) 192, 248
NdeII GATC 4 cut(s) 11, 112, 126, 429
NlaIII CATG 2 cut(s) 142, 508
NlaIV GGNNCC 1 cut(s) 265
NmeAIII GCCGAG 2 cut(s) 190, 250
NmuCI GTSAC 3 cut(s) 379, 394, 647
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PctI GAATGC 1 cut(s) 303
Pfl23II CGTACG 1 cut(s) 390
PkrI GCNGC 4 cut(s) 40, 324, 451, 604
PleI GAGTC 2 cut(s) 115, 248
PpsI GAGTC 2 cut(s) 115, 248
Ppu21I YACGTR 1 cut(s) 394
Psp6I CCWGG 1 cut(s) 176
PspGI CCWGG 1 cut(s) 176
PspLI CGTACG 1 cut(s) 390
PspN4I GGNNCC 1 cut(s) 265
PspPI GGNCC 2 cut(s) 79, 174
PstI CTGCAG 1 cut(s) 620
RsaI GTAC 5 cut(s) 149, 356, 392, 458, 738
RsaNI GTAC 5 cut(s) 148, 355, 391, 457, 737
RseI CAYNNNNRTG 2 cut(s) 248, 509
SaqAI TTAA 1 cut(s) 779
SatI GCNGC 4 cut(s) 39, 323, 450, 603
Sau3AI GATC 4 cut(s) 11, 112, 126, 429
Sau96I GGNCC 2 cut(s) 79, 174
SchI GAGTC 2 cut(s) 116, 249
ScrFI CCNGG 3 cut(s) 178, 192, 248
SduI GDGCHC 2 cut(s) 216, 568
SfcI CTRYAG 1 cut(s) 616
SinI GGWCC 1 cut(s) 174
SmiMI CAYNNNNRTG 2 cut(s) 248, 509
Sse9I AATT 4 cut(s) 529, 768, 781, 797
SsiI CCGC 6 cut(s) 21, 38, 157, 222, 345, 572
StyD4I CCNGG 3 cut(s) 176, 190, 246
StyI CCWWGG 1 cut(s) 688
TaaI ACNGT 4 cut(s) 283, 473, 481, 697
TaiI ACGT 4 cut(s) 189, 381, 396, 467
TaqI TCGA 4 cut(s) 14, 117, 204, 428
TasI AATT 4 cut(s) 529, 768, 781, 797
TatI WGTACW 2 cut(s) 147, 354
TauI GCSGC 1 cut(s) 41
Tru1I TTAA 1 cut(s) 779
Tru9I TTAA 1 cut(s) 779
TscAI CASTG 2 cut(s) 478, 700
TseFI GTSAC 3 cut(s) 379, 394, 647
TseI GCWGC 3 cut(s) 322, 449, 602
Tsp45I GTSAC 3 cut(s) 379, 394, 647
TspDTI ATGAA 4 cut(s) 493, 613, 673, 694
TspGWI ACGGA 1 cut(s) 297
TspRI CASTG 2 cut(s) 478, 700
VneI GTGCAC 1 cut(s) 564
VpaK11BI GGWCC 1 cut(s) 174
XapI RAATTY 3 cut(s) 529, 781, 797
XcmI CCANNNNNNNNNTGG 1 cut(s) 484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.