MD02G1100100.v1.1

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001024
Physical Location & Seq
Reverse (-)
7940742 .. 7944092
3351 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1100100.v1.1.491

Sequence Viewer

Length: 1101 bp
ATGGCACCACTATTAGCATTTCTTCTGGTTCTCAACATCTTCATAAATTCACCTCAACTCGGGCTGAGTCGCCCCAACCTCATCCCACCTAACTTCTCCCAACTCGACTCGTGGATCGCCCACAACATGAGGGACTACGCCAACCGCAAGGCCACCCAAACCACTCTCCGCTTCGACGCCCAACTTCTCTCTGCCGAGGAAATTGTCAGAATCATAACCGTCAGGAAAGACGGAACCGGCCAATTCAGAACCGTCACAGACGCAGTCAACAGCATTCCTTCTGGGAACACAAGGCGCGTCGTGGTTTTCATCGGCGGGGGCGTGTATACGGAGAAGGTTCTTGTTGACGCGTCAAAGCCGTTCGTGACGTTTTACGGGGACCAGAACGACGTGCCGTCAATCACGTTCGATGGCACGGCGTTGAAGTACGGGACGTGGAATAGTTCCACGGTGGCGGTGGAGTCCGACTACTTCGTGGCCGTCAATATTGCGTTCGTGAATTCAGCTCCGATGCCGGACGGAATAAGGCCGGGGGCACAGGCGGTGGCGATGAGGATATCAGGAGACAAGGCAGCGTTCCACAACTGCAGGTTCATAGGATTTCAGGACACGTTATTGGATGATAGGGGGAGACATTTCTTCAAGGATTGCCACGTCCAAGGCACGGTCGATTTCATCTTTGGCAATGGCAAATCCCTCTACTTGAATAATACGATACATTCAGTTGCAAATGGTATGGGGGTGATAACGGCACATGCTAGGGAAAATGTGGAAGACGATAGTGGTTTTGCGTTTGTGCATTGCAACATAACGGGGACAGGTGACACATTTCTTGGGCGAGCTTGGAGGGAGAGACCTAGGGTTGTGTTTGCTTACGCATACATGGGCTCTCTCATCAATAAACAAGGATGGTCTGACTACATGCATACCGAGCGCGATAAGACTGTGTATTATGGAGAGTACAAGTGTATGGGACCGGGTTCGAGCTCATCGGGTCGGGTCAAGTATGCAAGAATGTTATCTGATGAAGAAGCAAAGCCCTTTCTCAGCCTGACTTTCATCAAAGGAACAAAGTGGGTTCTCCCACCTCCCAAGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

367

Amino Acids

40.59

Weight (kDa)

8.76

Isoelectric Point (pI)

35.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 72 - 356 4.2e-71 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 579
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 326
AccII CGCG 3 cut(s) 297, 350, 936
AciI CCGC 5 cut(s) 145, 169, 315, 455, 542
AclWI GGATC 1 cut(s) 122
AcoI YGGCCR 2 cut(s) 238, 477
AcsI RAATTY 2 cut(s) 46, 499
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 2 cut(s) 428, 962
AfiI CCNNNNNNNGG 2 cut(s) 59, 664
AflIII ACRYGT 2 cut(s) 348, 609
AgsI TTSAA 3 cut(s) 424, 643, 706
AhdI GACNNNNNGTC 1 cut(s) 394
AjiI CACGTC 3 cut(s) 391, 435, 655
AluBI AGCT 4 cut(s) 506, 842, 987, 1096
AluI AGCT 4 cut(s) 506, 842, 987, 1096
Alw21I GWGCWC 1 cut(s) 989
Alw26I GTCTC 3 cut(s) 558, 625, 847
AlwI GGATC 1 cut(s) 122
Ama87I CYCGRG 1 cut(s) 59
AoxI GGCC 4 cut(s) 150, 238, 477, 527
ApeKI GCWGC 1 cut(s) 572
ApoI RAATTY 2 cut(s) 46, 499
AspA2I CCTAGG 1 cut(s) 857
AspLEI GCGC 2 cut(s) 297, 936
AspS9I GGNCC 2 cut(s) 379, 974
AsuC2I CCSGG 2 cut(s) 531, 978
AsuHPI GGTGA 3 cut(s) 42, 754, 833
AvaI CYCGRG 1 cut(s) 59
AvaII GGWCC 2 cut(s) 379, 974
AvrII CCTAGG 1 cut(s) 857
BaeGI GKGCMC 1 cut(s) 538
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 2 cut(s) 890, 989
BauI CACGAG 1 cut(s) 109
BbsI GAAGAC 1 cut(s) 780
Bbv12I GWGCWC 1 cut(s) 989
BbvI GCAGC 1 cut(s) 584
BccI CCATC 2 cut(s) 404, 903
BceAI ACGGC 5 cut(s) 343, 379, 432, 464, 765
BcnI CCSGG 2 cut(s) 531, 978
BcoDI GTCTC 3 cut(s) 558, 625, 847
BfaI CTAG 2 cut(s) 759, 858
BfmI CTRYAG 1 cut(s) 586
BfuAI ACCTGC 1 cut(s) 579
BisI GCNGC 1 cut(s) 573
BlnI CCTAGG 1 cut(s) 857
BlsI GCNGC 1 cut(s) 574
Bme1390I CCNGG 2 cut(s) 531, 978
Bme18I GGWCC 2 cut(s) 379, 974
BmeRI GACNNNNNGTC 1 cut(s) 394
BmeT110I CYCGRG 1 cut(s) 59
BmgBI CACGTC 3 cut(s) 391, 435, 655
BmgT120I GGNCC 2 cut(s) 379, 974
BmiI GGNNCC 4 cut(s) 6, 235, 380, 975
BmrFI CCNGG 2 cut(s) 531, 978
BmsI GCATC 1 cut(s) 501
BpiI GAAGAC 1 cut(s) 780
BpuMI CCSGG 2 cut(s) 531, 978
BsaHI GRCGYC 1 cut(s) 177
BsaI GGTCTC 1 cut(s) 847
BsaJI CCNNGG 5 cut(s) 195, 447, 530, 658, 857
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 664
Bse118I RCCGGY 1 cut(s) 236
Bse3DI GCAATG 2 cut(s) 691, 799
BseDI CCNNGG 5 cut(s) 195, 447, 530, 658, 857
BseGI GGATG 3 cut(s) 81, 625, 914
BseLI CCNNNNNNNGG 2 cut(s) 59, 664
BseMI GCAATG 2 cut(s) 691, 799
BseMII CTCAG 2 cut(s) 56, 1060
BseSI GKGCMC 1 cut(s) 538
BseXI GCAGC 1 cut(s) 584
Bsh1236I CGCG 3 cut(s) 297, 350, 936
Bsh1285I CGRYCG 1 cut(s) 669
BshFI GGCC 4 cut(s) 152, 240, 479, 529
BshNI GGYRCC 1 cut(s) 4
BsiEI CGRYCG 1 cut(s) 669
BsiHKAI GWGCWC 1 cut(s) 989
BsiHKCI CYCGRG 1 cut(s) 59
BsiSI CCGG 4 cut(s) 237, 515, 530, 977
BslFI GGGAC 5 cut(s) 146, 392, 445, 829, 987
BslI CCNNNNNNNGG 2 cut(s) 59, 664
BsmAI GTCTC 3 cut(s) 558, 625, 847
BsmFI GGGAC 5 cut(s) 146, 392, 445, 829, 987
BsmI GAATGC 1 cut(s) 273
BsnI GGCC 4 cut(s) 152, 240, 479, 529
Bso31I GGTCTC 1 cut(s) 847
BsoBI CYCGRG 1 cut(s) 59
Bsp1286I GDGCHC 3 cut(s) 538, 890, 989
Bsp143I GATC 1 cut(s) 114
BspACI CCGC 5 cut(s) 145, 169, 315, 455, 542
BspANI GGCC 4 cut(s) 152, 240, 479, 529
BspCNI CTCAG 2 cut(s) 57, 1059
BspFNI CGCG 3 cut(s) 297, 350, 936
BspLI GGNNCC 4 cut(s) 6, 235, 380, 975
BspMAI CTGCAG 1 cut(s) 590
BspMI ACCTGC 1 cut(s) 579
BspPI GGATC 1 cut(s) 122
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 847
BsrDI GCAATG 2 cut(s) 691, 799
BsrFI RCCGGY 1 cut(s) 236
BssAI RCCGGY 1 cut(s) 236
BssECI CCNNGG 5 cut(s) 195, 447, 530, 658, 857
BssMI GATC 1 cut(s) 114
BssNAI GTATAC 1 cut(s) 327
BssNI GRCGYC 1 cut(s) 177
BssSI CACGAG 1 cut(s) 109
BssT1I CCWWGG 2 cut(s) 658, 857
Bst1107I GTATAC 1 cut(s) 327
Bst2BI CACGAG 1 cut(s) 109
Bst4CI ACNGT 5 cut(s) 220, 253, 451, 667, 946
BstACI GRCGYC 1 cut(s) 177
BstC8I GCNNGC 1 cut(s) 840
BstDEI CTNAG 2 cut(s) 65, 1046
BstDSI CCRYGG 1 cut(s) 447
BstF5I GGATG 3 cut(s) 81, 625, 914
BstFNI CGCG 3 cut(s) 297, 350, 936
BstHHI GCGC 2 cut(s) 297, 936
BstKTI GATC 1 cut(s) 117
BstMAI GTCTC 3 cut(s) 558, 625, 847
BstMBI GATC 1 cut(s) 114
BstMCI CGRYCG 1 cut(s) 669
BstMWI GCNNNNNNNGC 1 cut(s) 931
BstNSI RCATGY 2 cut(s) 758, 925
BstSCI CCNGG 2 cut(s) 529, 976
BstSFI CTRYAG 1 cut(s) 586
BstSLI GKGCMC 1 cut(s) 538
BstUI CGCG 3 cut(s) 297, 350, 936
BstV1I GCAGC 1 cut(s) 584
BstV2I GAAGAC 1 cut(s) 780
BstZ17I GTATAC 1 cut(s) 327
BsuRI GGCC 4 cut(s) 152, 240, 479, 529
BtgI CCRYGG 1 cut(s) 447
BtgZI GCGATG 1 cut(s) 563
BtrI CACGTC 3 cut(s) 391, 435, 655
BtsCI GGATG 3 cut(s) 81, 625, 914
BveI ACCTGC 1 cut(s) 579
Cac8I GCNNGC 1 cut(s) 840
CfoI GCGC 2 cut(s) 297, 936
Cfr10I RCCGGY 1 cut(s) 236
Cfr13I GGNCC 2 cut(s) 379, 974
CseI GACGC 5 cut(s) 185, 269, 286, 339, 356
Csp6I GTAC 2 cut(s) 427, 961
CviAII CATG 4 cut(s) 127, 755, 883, 922
CviQI GTAC 2 cut(s) 427, 961
DdeI CTNAG 2 cut(s) 65, 1046
DpnI GATC 1 cut(s) 116
DpnII GATC 1 cut(s) 114
DriI GACNNNNNGTC 1 cut(s) 394
EaeI YGGCCR 2 cut(s) 238, 477
Eam1105I GACNNNNNGTC 1 cut(s) 394
Ecl136II GAGCTC 1 cut(s) 987
Eco130I CCWWGG 2 cut(s) 658, 857
Eco24I GRGCYC 2 cut(s) 890, 989
Eco31I GGTCTC 1 cut(s) 847
Eco32I GATATC 1 cut(s) 558
Eco47I GGWCC 2 cut(s) 379, 974
Eco53kI GAGCTC 1 cut(s) 987
Eco88I CYCGRG 1 cut(s) 59
EcoICRI GAGCTC 1 cut(s) 987
EcoRI GAATTC 1 cut(s) 499
EcoRV GATATC 1 cut(s) 558
EcoT14I CCWWGG 2 cut(s) 658, 857
EcoT22I ATGCAT 1 cut(s) 927
EcoT38I GRGCYC 2 cut(s) 890, 989
ErhI CCWWGG 2 cut(s) 658, 857
FaeI CATG 4 cut(s) 130, 758, 886, 925
FaqI GGGAC 5 cut(s) 146, 392, 445, 829, 987
FatI CATG 4 cut(s) 126, 754, 882, 921
FauI CCCGC 1 cut(s) 308
FblI GTMKAC 1 cut(s) 326
Fnu4HI GCNGC 1 cut(s) 573
FokI GGATG 3 cut(s) 68, 632, 921
FriOI GRGCYC 2 cut(s) 890, 989
Fsp4HI GCNGC 1 cut(s) 573
FspBI CTAG 2 cut(s) 759, 858
GlaI GCGC 2 cut(s) 296, 935
GluI GCNGC 1 cut(s) 573
HaeIII GGCC 4 cut(s) 152, 240, 479, 529
HapII CCGG 4 cut(s) 237, 515, 530, 977
HgaI GACGC 5 cut(s) 185, 269, 286, 339, 356
HhaI GCGC 2 cut(s) 297, 936
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 4 cut(s) 130, 758, 886, 925
Hin6I GCGC 2 cut(s) 295, 934
HinP1I GCGC 2 cut(s) 295, 934
HincII GTYRAC 2 cut(s) 268, 346
HindII GTYRAC 2 cut(s) 268, 346
HinfI GANTC 4 cut(s) 67, 107, 210, 461
HpaII CCGG 4 cut(s) 237, 515, 530, 977
HphI GGTGA 3 cut(s) 42, 754, 833
Hpy166II GTNNAC 3 cut(s) 268, 327, 346
Hpy188I TCNGA 7 cut(s) 209, 248, 466, 510, 916, 1024, 1100
Hpy188III TCNNGA 5 cut(s) 223, 364, 496, 561, 605
Hpy8I GTNNAC 3 cut(s) 268, 327, 346
Hpy99I CGWCG 3 cut(s) 179, 302, 392
HpyAV CCTTC 2 cut(s) 288, 328
HpyCH4III ACNGT 5 cut(s) 220, 253, 451, 667, 946
HpyCH4IV ACGT 6 cut(s) 368, 390, 404, 434, 611, 654
HpyCH4V TGCA 6 cut(s) 588, 728, 799, 804, 925, 1010
HpyF10VI GCNNNNNNNGC 1 cut(s) 931
HpyF3I CTNAG 2 cut(s) 65, 1046
HpySE526I ACGT 6 cut(s) 368, 390, 404, 434, 611, 654
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 4 cut(s) 130, 758, 886, 925
HspAI GCGC 2 cut(s) 295, 934
Kzo9I GATC 1 cut(s) 114
LmnI GCTCC 1 cut(s) 511
Lsp1109I GCAGC 1 cut(s) 584
LweI GCATC 1 cut(s) 501
MaeI CTAG 2 cut(s) 759, 858
MaeII ACGT 6 cut(s) 368, 390, 404, 434, 611, 654
MaeIII GTNAC 3 cut(s) 253, 364, 821
MalI GATC 1 cut(s) 116
MboI GATC 1 cut(s) 114
MboII GAAGA 5 cut(s) 14, 31, 631, 785, 1040
MhlI GDGCHC 3 cut(s) 538, 890, 989
MluCI AATT 4 cut(s) 46, 201, 242, 499
MluI ACGCGT 1 cut(s) 348
MlyI GAGTC 3 cut(s) 76, 101, 470
MmeI TCCRAC 1 cut(s) 489
MnlI CCTC 8 cut(s) 63, 89, 123, 190, 546, 707, 840, 1098
Mph1103I ATGCAT 1 cut(s) 927
MspI CCGG 4 cut(s) 237, 515, 530, 977
MspR9I CCNGG 2 cut(s) 531, 978
Mva1269I GAATGC 1 cut(s) 273
MvnI CGCG 3 cut(s) 297, 350, 936
MwoI GCNNNNNNNGC 1 cut(s) 931
NciI CCSGG 2 cut(s) 531, 978
NdeII GATC 1 cut(s) 114
NlaIII CATG 4 cut(s) 130, 758, 886, 925
NlaIV GGNNCC 4 cut(s) 6, 235, 380, 975
NmeAIII GCCGAG 1 cut(s) 220
NmuCI GTSAC 3 cut(s) 253, 364, 821
NsiI ATGCAT 1 cut(s) 927
NspI RCATGY 2 cut(s) 758, 925
PcsI WCGNNNNNNNCGW 1 cut(s) 318
PctI GAATGC 1 cut(s) 273
PfeI GAWTC 1 cut(s) 210
PflFI GACNNNGTC 1 cut(s) 263
PkrI GCNGC 1 cut(s) 574
PleI GAGTC 3 cut(s) 75, 101, 469
PpsI GAGTC 3 cut(s) 75, 101, 469
Psp124BI GAGCTC 1 cut(s) 989
PspN4I GGNNCC 4 cut(s) 6, 235, 380, 975
PspPI GGNCC 2 cut(s) 379, 974
PstI CTGCAG 1 cut(s) 590
PsyI GACNNNGTC 1 cut(s) 263
RsaI GTAC 2 cut(s) 428, 962
RsaNI GTAC 2 cut(s) 427, 961
SacI GAGCTC 1 cut(s) 989
SatI GCNGC 1 cut(s) 573
Sau3AI GATC 1 cut(s) 114
Sau96I GGNCC 2 cut(s) 379, 974
SchI GAGTC 3 cut(s) 76, 101, 470
ScrFI CCNGG 2 cut(s) 531, 978
SduI GDGCHC 3 cut(s) 538, 890, 989
SfaNI GCATC 1 cut(s) 501
SfcI CTRYAG 1 cut(s) 586
SinI GGWCC 2 cut(s) 379, 974
Sse9I AATT 4 cut(s) 46, 201, 242, 499
SsiI CCGC 5 cut(s) 145, 169, 315, 455, 542
SspI AATATT 1 cut(s) 487
SspMI CTAG 2 cut(s) 759, 858
SstI GAGCTC 1 cut(s) 989
StyD4I CCNGG 2 cut(s) 529, 976
StyI CCWWGG 2 cut(s) 658, 857
TaaI ACNGT 5 cut(s) 220, 253, 451, 667, 946
TaiI ACGT 6 cut(s) 371, 393, 407, 437, 614, 657
TaqI TCGA 5 cut(s) 105, 174, 408, 669, 983
TasI AATT 4 cut(s) 46, 201, 242, 499
TatI WGTACW 1 cut(s) 960
TfiI GAWTC 1 cut(s) 210
TseFI GTSAC 3 cut(s) 253, 364, 821
TseI GCWGC 1 cut(s) 572
Tsp45I GTSAC 3 cut(s) 253, 364, 821
TspDTI ATGAA 6 cut(s) 31, 298, 583, 664, 1041, 1048
TspGWI ACGGA 3 cut(s) 246, 344, 534
Tth111I GACNNNGTC 1 cut(s) 263
VpaK11BI GGWCC 2 cut(s) 379, 974
XapI RAATTY 2 cut(s) 46, 499
XceI RCATGY 2 cut(s) 758, 925
XcmI CCANNNNNNNNNTGG 1 cut(s) 454
XmaJI CCTAGG 1 cut(s) 857
XmiI GTMKAC 1 cut(s) 326
XspI CTAG 2 cut(s) 759, 858
Zsp2I ATGCAT 1 cut(s) 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.