Rh2CG106300

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0001024
Physical Location & Seq
Reverse (-)
9171944 .. 9175562
3619 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG106300.1

Sequence Viewer

Length: 1032 bp
ATGGCTATGAGATCGACACCCGCTCTTGTGGTCGTTGCGGCATTTCTGGCTCTCCATTTCGTGCCTTTGCTCGTTTCTGGCCACCCCAACCTCATTCCGGAAAACGAGTCTGATCTCGATGGCTGGATCGTCCAAAACATGAGGGAGTACAACGAGCGGAAGGCCGAGCTGAGGACCAGGAGCAACGTCCCCGGCACGCACTTCGACACAGGGCTCGCCGCCGCCGAGGACGCAAAGAGAGTCATCCGGGTGAAGCAGGACGGCACAGGAGACTTTAGGACGGTGACGGACGCCGTGAGGAGCATTCCGTCTGCAAACAAGCAGCGCGTGGTGGTGTGGATTGGCGGGGGAGAGTACAAGGAGAAGGTTCTGGTGGACGTGACGAAGCCGTACGTGACGTTTTTTGGGGACAAGAACAACATCCCGTCGATCACCTTCGACGGCACGGCTGCCGTGTACGGGACGTGGAACAGTGCCACGGTGGCGGTGGAAGCCGACTATTTCATGGCCGTGAATATAGCGTTCGTGAATTCAGCTCCTATGCCGGACGGCATAAGGAAAGGTGCTCAGGCGGTGGCAATGAGAATATCAGGGGATAAGGCAGCCTTCCACAACTGCAGGTTCATCGGATTTCAGGACACCTTATGTGACGATAGGGGAAGGCATCTCTTCAAGGATTGCTTCATCCAAGGCACTGTTGATTTTATCTTTGGCAATGGAAAATCCCTCTACTTGAAAAGTACCTTAAACTCAGTTGCGAATGGAAATGGGGTGATCACGGCACATGGTAGAGCAGATGTGCAGGATGATAGCGGATTTTCGTTTGTGTATTGCAACATAACGGGGACAGGTGAGACCTTGCTTGGGCGTGCTTGGAGGGGAAGAGCAAGGGTGGTGTTTTCTTATACATACATGGGGAACCTTGTCGACAGAGAAGGGTGGTCCGACTACAACGACAGTGCGCGTGCCAAGTACGTTCATTTCTATATCTCTAGTTTTCATAATATGACAAATATATGTAAAATGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

343

Amino Acids

37.91

Weight (kDa)

8.5

Isoelectric Point (pI)

24.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 83 - 327 2.3e-57 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 609
AccBSI CCGCTC 2 cut(s) 23, 157
AccI GTMKAC 1 cut(s) 927
AccII CGCG 2 cut(s) 327, 964
AccIII TCCGGA 1 cut(s) 97
AciI CCGC 9 cut(s) 21, 38, 157, 219, 222, 345, 485, 572, 813
AclWI GGATC 1 cut(s) 134
AcoI YGGCCR 2 cut(s) 79, 507
AcsI RAATTY 1 cut(s) 529
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 6 cut(s) 149, 356, 392, 458, 742, 974
AfiI CCNNNNNNNGG 4 cut(s) 97, 171, 459, 864
AgsI TTSAA 2 cut(s) 673, 736
AjiI CACGTC 2 cut(s) 379, 465
AjnI CCWGG 1 cut(s) 176
AluBI AGCT 2 cut(s) 169, 536
AluI AGCT 2 cut(s) 169, 536
Alw21I GWGCWC 1 cut(s) 568
Alw26I GTCTC 2 cut(s) 264, 848
AlwI GGATC 1 cut(s) 134
Aor13HI TCCGGA 1 cut(s) 97
AoxI GGCC 3 cut(s) 79, 162, 507
ApeKI GCWGC 3 cut(s) 322, 449, 602
ApoI RAATTY 1 cut(s) 529
AspLEI GCGC 2 cut(s) 327, 964
AspS9I GGNCC 2 cut(s) 174, 942
AsuC2I CCSGG 2 cut(s) 192, 248
AsuHPI GGTGA 5 cut(s) 262, 295, 424, 784, 863
AvaII GGWCC 2 cut(s) 174, 942
BalI TGGCCA 1 cut(s) 81
BanII GRGCYC 1 cut(s) 216
Bbv12I GWGCWC 1 cut(s) 568
BbvCI CCTCAGC 1 cut(s) 170
BbvI GCAGC 3 cut(s) 334, 436, 614
BccI CCATC 1 cut(s) 113
BceAI ACGGC 9 cut(s) 277, 278, 373, 437, 457, 462, 494, 565, 795
BcgI CGANNNNNNTGC 4 cut(s) 184, 218, 607, 641
BciT130I CCWGG 1 cut(s) 178
BclI TGATCA 1 cut(s) 774
BcnI CCSGG 2 cut(s) 192, 248
BcoDI GTCTC 2 cut(s) 264, 848
BfaI CTAG 1 cut(s) 993
BfmI CTRYAG 1 cut(s) 616
BfuAI ACCTGC 1 cut(s) 609
BglI GCCNNNNNGGC 1 cut(s) 482
BisI GCNGC 6 cut(s) 39, 219, 222, 323, 450, 603
BlsI GCNGC 6 cut(s) 40, 220, 223, 324, 451, 604
Bme1390I CCNGG 3 cut(s) 178, 192, 248
Bme18I GGWCC 2 cut(s) 174, 942
BmgBI CACGTC 2 cut(s) 379, 465
BmgT120I GGNCC 2 cut(s) 174, 942
BmiI GGNNCC 1 cut(s) 920
BmrFI CCNGG 3 cut(s) 178, 192, 248
BmsI GCATC 1 cut(s) 673
Bpu10I CCTNAGC 2 cut(s) 170, 567
BpuMI CCSGG 2 cut(s) 192, 248
BsaAI YACGTR 1 cut(s) 394
BsaHI GRCGYC 1 cut(s) 291
BsaI GGTCTC 1 cut(s) 848
BsaJI CCNNGG 4 cut(s) 190, 225, 477, 688
BsaWI WCCGGW 1 cut(s) 97
Bsc4I CCNNNNNNNGG 4 cut(s) 97, 171, 459, 864
Bse3DI GCAATG 2 cut(s) 585, 721
BseAI TCCGGA 1 cut(s) 97
BseBI CCWGG 1 cut(s) 178
BseDI CCNNGG 4 cut(s) 190, 225, 477, 688
BseGI GGATG 4 cut(s) 243, 420, 684, 811
BseLI CCNNNNNNNGG 4 cut(s) 97, 171, 459, 864
BseMI GCAATG 2 cut(s) 585, 721
BseMII CTCAG 3 cut(s) 161, 581, 765
BseRI GAGGAG 1 cut(s) 313
BseXI GCAGC 3 cut(s) 334, 436, 614
BsgI GTGCAG 1 cut(s) 821
Bsh1236I CGCG 2 cut(s) 327, 964
BshFI GGCC 3 cut(s) 81, 164, 509
BsiHKAI GWGCWC 1 cut(s) 568
BsiSI CCGG 4 cut(s) 98, 192, 247, 545
BsiWI CGTACG 1 cut(s) 390
BslFI GGGAC 4 cut(s) 173, 422, 475, 859
BslI CCNNNNNNNGG 4 cut(s) 97, 171, 459, 864
BsmAI GTCTC 2 cut(s) 264, 848
BsmFI GGGAC 4 cut(s) 173, 422, 475, 859
BsmI GAATGC 1 cut(s) 303
BsnI GGCC 3 cut(s) 81, 164, 509
Bso31I GGTCTC 1 cut(s) 848
Bsp1286I GDGCHC 2 cut(s) 216, 568
Bsp13I TCCGGA 1 cut(s) 97
Bsp143I GATC 5 cut(s) 11, 112, 126, 429, 774
BspACI CCGC 9 cut(s) 21, 38, 157, 219, 222, 345, 485, 572, 813
BspANI GGCC 3 cut(s) 81, 164, 509
BspCNI CTCAG 3 cut(s) 162, 580, 764
BspEI TCCGGA 1 cut(s) 97
BspFNI CGCG 2 cut(s) 327, 964
BspLI GGNNCC 1 cut(s) 920
BspMAI CTGCAG 1 cut(s) 620
BspMI ACCTGC 1 cut(s) 609
BspPI GGATC 1 cut(s) 134
BspQI GCTCTTC 1 cut(s) 877
BspTNI GGTCTC 1 cut(s) 848
BsrBI CCGCTC 2 cut(s) 23, 157
BsrDI GCAATG 2 cut(s) 585, 721
BssECI CCNNGG 4 cut(s) 190, 225, 477, 688
BssMI GATC 5 cut(s) 11, 112, 126, 429, 774
BssNI GRCGYC 1 cut(s) 291
BssT1I CCWWGG 1 cut(s) 688
Bst2UI CCWGG 1 cut(s) 178
Bst4CI ACNGT 5 cut(s) 283, 473, 481, 697, 959
Bst6I CTCTTC 2 cut(s) 674, 877
BstACI GRCGYC 1 cut(s) 291
BstBAI YACGTR 1 cut(s) 394
BstC8I GCNNGC 4 cut(s) 197, 216, 870, 966
BstDEI CTNAG 3 cut(s) 170, 567, 751
BstDSI CCRYGG 1 cut(s) 477
BstF5I GGATG 4 cut(s) 243, 420, 684, 811
BstFNI CGCG 2 cut(s) 327, 964
BstHHI GCGC 2 cut(s) 327, 964
BstKTI GATC 5 cut(s) 14, 115, 129, 432, 777
BstMAI GTCTC 2 cut(s) 264, 848
BstMBI GATC 5 cut(s) 11, 112, 126, 429, 774
BstMWI GCNNNNNNNGC 4 cut(s) 47, 230, 482, 491
BstNI CCWGG 1 cut(s) 178
BstSCI CCNGG 3 cut(s) 176, 190, 246
BstSFI CTRYAG 1 cut(s) 616
BstUI CGCG 2 cut(s) 327, 964
BstV1I GCAGC 3 cut(s) 334, 436, 614
BsuRI GGCC 3 cut(s) 81, 164, 509
BtgI CCRYGG 1 cut(s) 477
BtrI CACGTC 2 cut(s) 379, 465
BtsCI GGATG 4 cut(s) 243, 420, 684, 811
BtsIMutI CAGTG 3 cut(s) 478, 693, 964
BveI ACCTGC 1 cut(s) 609
Cac8I GCNNGC 4 cut(s) 197, 216, 870, 966
CfoI GCGC 2 cut(s) 327, 964
Cfr13I GGNCC 2 cut(s) 174, 942
CseI GACGC 2 cut(s) 239, 299
Csp6I GTAC 6 cut(s) 148, 355, 391, 457, 741, 973
CviAII CATG 4 cut(s) 139, 505, 785, 913
CviQI GTAC 6 cut(s) 148, 355, 391, 457, 741, 973
DdeI CTNAG 3 cut(s) 170, 567, 751
DpnI GATC 5 cut(s) 13, 114, 128, 431, 776
DpnII GATC 5 cut(s) 11, 112, 126, 429, 774
EaeI YGGCCR 2 cut(s) 79, 507
Eam1104I CTCTTC 2 cut(s) 674, 877
EarI CTCTTC 2 cut(s) 674, 877
Eco130I CCWWGG 1 cut(s) 688
Eco24I GRGCYC 1 cut(s) 216
Eco31I GGTCTC 1 cut(s) 848
Eco47I GGWCC 2 cut(s) 174, 942
EcoRI GAATTC 1 cut(s) 529
EcoRII CCWGG 1 cut(s) 176
EcoT14I CCWWGG 1 cut(s) 688
EcoT38I GRGCYC 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 688
FaeI CATG 4 cut(s) 142, 508, 788, 916
FalI AAGNNNNNCTT 4 cut(s) 590, 622, 665, 697
FaqI GGGAC 4 cut(s) 173, 422, 475, 859
FatI CATG 4 cut(s) 138, 504, 784, 912
FauI CCCGC 2 cut(s) 28, 338
FbaI TGATCA 1 cut(s) 774
FblI GTMKAC 1 cut(s) 927
Fnu4HI GCNGC 6 cut(s) 39, 219, 222, 323, 450, 603
FokI GGATG 4 cut(s) 230, 407, 671, 818
FriOI GRGCYC 1 cut(s) 216
Fsp4HI GCNGC 6 cut(s) 39, 219, 222, 323, 450, 603
FspBI CTAG 1 cut(s) 993
GlaI GCGC 2 cut(s) 326, 963
GluI GCNGC 6 cut(s) 39, 219, 222, 323, 450, 603
HaeIII GGCC 3 cut(s) 81, 164, 509
HapII CCGG 4 cut(s) 98, 192, 247, 545
HgaI GACGC 2 cut(s) 239, 299
HhaI GCGC 2 cut(s) 327, 964
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 4 cut(s) 142, 508, 788, 916
Hin6I GCGC 2 cut(s) 325, 962
HinP1I GCGC 2 cut(s) 325, 962
HincII GTYRAC 1 cut(s) 928
HindII GTYRAC 1 cut(s) 928
HinfI GANTC 2 cut(s) 107, 240
HpaII CCGG 4 cut(s) 98, 192, 247, 545
HphI GGTGA 5 cut(s) 262, 295, 424, 784, 863
Hpy166II GTNNAC 3 cut(s) 376, 457, 928
Hpy188I TCNGA 3 cut(s) 112, 629, 946
Hpy188III TCNNGA 4 cut(s) 98, 116, 526, 635
Hpy8I GTNNAC 3 cut(s) 376, 457, 928
Hpy99I CGWCG 2 cut(s) 430, 443
HpyAV CCTTC 6 cut(s) 154, 358, 445, 616, 654, 929
HpyCH4III ACNGT 5 cut(s) 283, 473, 481, 697, 959
HpyCH4IV ACGT 6 cut(s) 186, 378, 393, 398, 464, 975
HpyCH4V TGCA 4 cut(s) 314, 618, 802, 834
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 230, 482, 491
HpyF3I CTNAG 3 cut(s) 170, 567, 751
HpySE526I ACGT 6 cut(s) 186, 378, 393, 398, 464, 975
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 4 cut(s) 142, 508, 788, 916
HspAI GCGC 2 cut(s) 325, 962
Kpn2I TCCGGA 1 cut(s) 97
Ksp22I TGATCA 1 cut(s) 774
Kzo9I GATC 5 cut(s) 11, 112, 126, 429, 774
LguI GCTCTTC 1 cut(s) 877
LmnI GCTCC 3 cut(s) 180, 300, 541
Lsp1109I GCAGC 3 cut(s) 334, 436, 614
LweI GCATC 1 cut(s) 673
MaeI CTAG 1 cut(s) 993
MaeII ACGT 6 cut(s) 186, 378, 393, 398, 464, 975
MaeIII GTNAC 4 cut(s) 283, 379, 394, 647
MalI GATC 5 cut(s) 13, 114, 128, 431, 776
MbiI CCGCTC 2 cut(s) 23, 157
MboI GATC 5 cut(s) 11, 112, 126, 429, 774
MboII GAAGA 2 cut(s) 661, 894
MhlI GDGCHC 2 cut(s) 216, 568
MlsI TGGCCA 1 cut(s) 81
MluCI AATT 1 cut(s) 529
MluNI TGGCCA 1 cut(s) 81
MlyI GAGTC 2 cut(s) 116, 249
MmeI TCCRAC 1 cut(s) 969
MnlI CCTC 7 cut(s) 101, 135, 165, 220, 291, 737, 870
Mox20I TGGCCA 1 cut(s) 81
MroI TCCGGA 1 cut(s) 97
MscI TGGCCA 1 cut(s) 81
MseI TTAA 1 cut(s) 746
MslI CAYNNNNRTG 2 cut(s) 248, 509
Msp20I TGGCCA 1 cut(s) 81
MspI CCGG 4 cut(s) 98, 192, 247, 545
MspR9I CCNGG 3 cut(s) 178, 192, 248
Mva1269I GAATGC 1 cut(s) 303
MvaI CCWGG 1 cut(s) 178
MvnI CGCG 2 cut(s) 327, 964
MwoI GCNNNNNNNGC 4 cut(s) 47, 230, 482, 491
NciI CCSGG 2 cut(s) 192, 248
NdeII GATC 5 cut(s) 11, 112, 126, 429, 774
NlaIII CATG 4 cut(s) 142, 508, 788, 916
NlaIV GGNNCC 1 cut(s) 920
NmeAIII GCCGAG 2 cut(s) 190, 250
NmuCI GTSAC 4 cut(s) 283, 379, 394, 647
PciSI GCTCTTC 1 cut(s) 877
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PctI GAATGC 1 cut(s) 303
Pfl23II CGTACG 1 cut(s) 390
PkrI GCNGC 6 cut(s) 40, 220, 223, 324, 451, 604
PleI GAGTC 2 cut(s) 115, 248
PpsI GAGTC 2 cut(s) 115, 248
Ppu21I YACGTR 1 cut(s) 394
Psp6I CCWGG 1 cut(s) 176
PspGI CCWGG 1 cut(s) 176
PspLI CGTACG 1 cut(s) 390
PspN4I GGNNCC 1 cut(s) 920
PspPI GGNCC 2 cut(s) 174, 942
PstI CTGCAG 1 cut(s) 620
RsaI GTAC 6 cut(s) 149, 356, 392, 458, 742, 974
RsaNI GTAC 6 cut(s) 148, 355, 391, 457, 741, 973
RseI CAYNNNNRTG 2 cut(s) 248, 509
SalI GTCGAC 1 cut(s) 926
SapI GCTCTTC 1 cut(s) 877
SaqAI TTAA 1 cut(s) 746
SatI GCNGC 6 cut(s) 39, 219, 222, 323, 450, 603
Sau3AI GATC 5 cut(s) 11, 112, 126, 429, 774
Sau96I GGNCC 2 cut(s) 174, 942
SchI GAGTC 2 cut(s) 116, 249
ScrFI CCNGG 3 cut(s) 178, 192, 248
SduI GDGCHC 2 cut(s) 216, 568
SfaNI GCATC 1 cut(s) 673
SfcI CTRYAG 1 cut(s) 616
SinI GGWCC 2 cut(s) 174, 942
SmiMI CAYNNNNRTG 2 cut(s) 248, 509
Sse9I AATT 1 cut(s) 529
SsiI CCGC 9 cut(s) 21, 38, 157, 219, 222, 345, 485, 572, 813
SspMI CTAG 1 cut(s) 993
StyD4I CCNGG 3 cut(s) 176, 190, 246
StyI CCWWGG 1 cut(s) 688
TaaI ACNGT 5 cut(s) 283, 473, 481, 697, 959
TaiI ACGT 6 cut(s) 189, 381, 396, 401, 467, 978
TaqI TCGA 6 cut(s) 14, 117, 204, 428, 438, 927
TasI AATT 1 cut(s) 529
TatI WGTACW 2 cut(s) 147, 354
TauI GCSGC 3 cut(s) 41, 221, 224
Tru1I TTAA 1 cut(s) 746
Tru9I TTAA 1 cut(s) 746
TscAI CASTG 3 cut(s) 478, 700, 964
TseFI GTSAC 4 cut(s) 283, 379, 394, 647
TseI GCWGC 3 cut(s) 322, 449, 602
Tsp45I GTSAC 4 cut(s) 283, 379, 394, 647
TspDTI ATGAA 5 cut(s) 493, 613, 673, 968, 989
TspGWI ACGGA 2 cut(s) 297, 302
TspRI CASTG 3 cut(s) 478, 700, 964
VpaK11BI GGWCC 2 cut(s) 174, 942
XapI RAATTY 1 cut(s) 529
XcmI CCANNNNNNNNNTGG 1 cut(s) 484
XmiI GTMKAC 1 cut(s) 927
XspI CTAG 1 cut(s) 993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.