Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0001024

Physical Location & Seq

Genomic Coordinates Reverse (-)

27502676 .. 27504570

Gene Structure 1895 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1173200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1092 bp

ATGGTCGTCCATGCAGCTTTAATCATGACAATTCTCCTCTCTGCCACCATTGCCATGGCGGACGACAATACACCGGTACCAGCCGCCCTTTCCCAAGTCAACACGTGGTTCAATAACAATGTGAAGCCGTACACACAGCGGCAGAAAACGCTTGACCCTGCCGTTGTGACGGCCGAGGCTGGTCAAAAAGTCATCAAGGTCATGCAAGATGGAAAAGGAAACTTCAAGACCGTCACCGACGCCATTAATAGCATTCCGGCCGGGAATACCAAACGTGTCATTGTGTACATTGGATCAGGAGAGTACAAAGAAAAAATTACGATTCCGCGAAATAAACCATTTGTCACATTATATGGTTCTCCAACGAATATGCCAATTTTGACATTTGATGGCACGGCCCAGAAGTATGGAACTGTAAACAGTGCTACAGTGATCGCAGAATCCGATTACTTTGTTGCTGCTAATCTTATTATTAAAAACTCTTCACCAAGGCCAGATGGGAAAAGAGTGGGAGCGCAGGCAGTGGCATTGAGGGTGTCGGGGAACAAAGCAGCCTTGTACAATTGCAAGCTGTTTGGGTTTCAGGACACCCTTTGTGATGACAGGGGCAATCATTTGTTCAAGGACTGCTACATTGAAGGCACTGTGGATTTCATTTGGGGAAGTGGAAAGTCTCTCTATTTGAACACTGAGTTGCATGTGCTGGGGGATAATGGAATAACGGTGATAACAGCACAGGCAAGGGACTCAGATTCGGAGGACACTGGATACTCATTTGTTCACTGCAAGATAACTGGGACAGGCATTGGCACATACTTGGGAAGGGCTTGGAGGGCCAGACCCCAGGTGGTGTATGCATACACCAACATGACCAAGGTGGTAAACCCTGCCGGATGGAGCAACGATAACCACTCTGAACGCGACAAAACTGTGTCCTACGGAGAGTACAAGTGCATGGGTCCAGGTTCAAGTAAGAATAATAAGAGAAATTTGAGCAAAGAGCTGACTGACGAACAAGCCAAGCGTTTCATTAGCCTTGGCTATATTCAGGGTTCCAAATGGCTGCTTCCTCCTCCAAATCCAAAAGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006950 GO:0006952 GO:0008150 GO:0009505 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0012505 GO:0016043 GO:0016049 GO:0016787 GO:0016788 GO:0022414 GO:0030154 GO:0030312 GO:0030599 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0042742 GO:0042995 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0048046 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0050829 GO:0050896 GO:0051704 GO:0051707 GO:0052689 GO:0060560 GO:0071840 GO:0071944 GO:0090406 GO:0098542 GO:0120025

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

Pectinesterase

Protein Analysis

364

Amino Acids

39.7

Weight (kDa)

9.2

Isoelectric Point (pI)

21.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pectinesterase	PF01095	66 - 351	2e-60	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001024)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69940 AT5G07410 AT5G07420 AT5G07430 AT5G61680
fragaria_vesca	FvH4_1g09290 FvH4_1g11590 FvH4_7g26440
malus_domestica	MD01G1173100.v1.1 MD01G1173200.v1.1 MD02G1100100.v1.1 MD02G1142600.v1.1 MD06G1150400.v1.1 MD07G1239900.v1.1
prunus_persica	Prupe.1G537900_v2.0.a1 Prupe.1G538100_v2.0.a1 Prupe.1G538100_v2.0.a1 Prupe.2G268200_v2.0.a1 Prupe.2G268300_v2.0.a1 Prupe.2G268500_v2.0.a1 Prupe.3G147000_v2.0.a1 Prupe.3G149100_v2.0.a1 Prupe.6G128000_v2.0.a1 Prupe.6G128100_v2.0.a1 Prupe.6G128200_v2.0.a1 Prupe.6G128300_v2.0.a1 Prupe.7G011200_v2.0.a1 Prupe.7G192800_v2.0.a1 Prupe.I005000_v2.0.a1 Prupe.I005200_v2.0.a1
pyrus_communis	pycom07g21580
rosa_chinensis	RchiOBHm_Chr1g0373011 RchiOBHm_Chr1g0373031 RchiOBHm_Chr2g0095851
rosa_laevigata	RLG00000016600 RLG00000026857 RLG00000026858
rosa_multiflora	Rmu_co8342511.1_g000001 Rmu_co8345149.1_g000001 Rmu_sc0000496.1_g000005 Rmu_ssc0000425.1_g000010 Rmu_ssc0000425.1_g000011
rosa_roxburghii	Rroxscaffold_2G00146030 Rroxscaffold_4G00284530 Rroxscaffold_4G00284540
rosa_rugosa	Rorug01G0375500 Rorug02G0053300
rosa_samantha	Rh1AG383800 Rh1BG347700 Rh1BG347800 Rh1CG361100 Rh1CG361200 Rh1DG378600 Rh1DG378700 Rh2AG102400 Rh2BG103800 Rh2CG106300 Rh2DG103900
rosa_wichuraiana	Rw1G033830 Rw1G033840 Rw2G007790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	76
AccB1I	GGYRCC	1 cut(s)	76
AccII	CGCG	2 cut(s)	328, 921
AciI	CCGC	4 cut(s)	59, 84, 139, 326
AclWI	GGATC	1 cut(s)	301
AcoI	YGGCCR	2 cut(s)	171, 258
AcsI	RAATTY	1 cut(s)	988
AcvI	CACGTG	1 cut(s)	105
AcyI	GRCGYC	1 cut(s)	240
AfaI	GTAC	6 cut(s)	78, 131, 287, 305, 560, 947
AflIII	ACRYGT	2 cut(s)	102, 274
AgeI	ACCGGT	1 cut(s)	73
AgsI	TTSAA	6 cut(s)	112, 226, 622, 638, 685, 969
AjnI	CCWGG	2 cut(s)	843, 961
AluBI	AGCT	3 cut(s)	17, 571, 1003
AluI	AGCT	3 cut(s)	17, 571, 1003
Alw26I	GTCTC	1 cut(s)	678
AlwI	GGATC	1 cut(s)	301
AoxI	GGCC	5 cut(s)	171, 258, 396, 491, 834
ApeKI	GCWGC	4 cut(s)	14, 458, 551, 1063
ApoI	RAATTY	1 cut(s)	988
AseI	ATTAAT	1 cut(s)	246
AsiGI	ACCGGT	1 cut(s)	73
Asp718I	GGTACC	1 cut(s)	76
AspLEI	GCGC	1 cut(s)	517
AspS9I	GGNCC	3 cut(s)	397, 834, 959
AsuC2I	CCSGG	1 cut(s)	262
AsuHPI	GGTGA	3 cut(s)	226, 477, 736
AvaII	GGWCC	1 cut(s)	959
BaeI	ACNNNNGTAYC	2 cut(s)	60, 93
BanI	GGYRCC	1 cut(s)	76
BbrPI	CACGTG	1 cut(s)	105
BbvI	GCAGC	4 cut(s)	26, 445, 563, 1050
BccI	CCATC	4 cut(s)	203, 383, 491, 888
BceAI	ACGGC	4 cut(s)	112, 146, 186, 411
BciT130I	CCWGG	2 cut(s)	845, 963
BciVI	GTATCC	1 cut(s)	761
BcnI	CCSGG	1 cut(s)	262
BcoDI	GTCTC	1 cut(s)	678
BfmI	CTRYAG	1 cut(s)	426
BfuI	GTATCC	1 cut(s)	761
BisI	GCNGC	6 cut(s)	15, 84, 140, 459, 552, 1064
BlsI	GCNGC	6 cut(s)	16, 85, 141, 460, 553, 1065
Bme1390I	CCNGG	3 cut(s)	262, 845, 963
Bme18I	GGWCC	1 cut(s)	959
BmgT120I	GGNCC	3 cut(s)	397, 834, 959
BmiI	GGNNCC	3 cut(s)	78, 960, 1054
BmrFI	CCNGG	3 cut(s)	262, 845, 963
BmrI	ACTGGG	1 cut(s)	804
BmuI	ACTGGG	1 cut(s)	804
BpuMI	CCSGG	1 cut(s)	262
BsaAI	YACGTR	1 cut(s)	105
BsaHI	GRCGYC	1 cut(s)	240
BsaJI	CCNNGG	6 cut(s)	54, 174, 488, 843, 873, 1036
BsaWI	WCCGGW	1 cut(s)	73
Bse118I	RCCGGY	1 cut(s)	73
Bse1I	ACTGG	2 cut(s)	769, 799
Bse3DI	GCAATG	1 cut(s)	48
BseBI	CCWGG	2 cut(s)	845, 963
BseDI	CCNNGG	6 cut(s)	54, 174, 488, 843, 873, 1036
BseGI	GGATG	1 cut(s)	899
BseMI	GCAATG	1 cut(s)	48
BseMII	CTCAG	2 cut(s)	681, 762
BseNI	ACTGG	2 cut(s)	769, 799
BseRI	GAGGAG	2 cut(s)	26, 1062
BseX3I	CGGCCG	2 cut(s)	171, 258
BseXI	GCAGC	4 cut(s)	26, 445, 563, 1050
BseYI	CCCAGC	1 cut(s)	703
Bsh1236I	CGCG	2 cut(s)	328, 921
Bsh1285I	CGRYCG	2 cut(s)	174, 261
BshFI	GGCC	5 cut(s)	173, 260, 398, 493, 836
BshNI	GGYRCC	1 cut(s)	76
BshTI	ACCGGT	1 cut(s)	73
BsiEI	CGRYCG	2 cut(s)	174, 261
BsiSI	CCGG	4 cut(s)	74, 257, 261, 891
BslFI	GGGAC	2 cut(s)	758, 811
BsmAI	GTCTC	1 cut(s)	678
BsmFI	GGGAC	2 cut(s)	758, 811
BsmI	GAATGC	1 cut(s)	252
BsnI	GGCC	5 cut(s)	173, 260, 398, 493, 836
Bsp1407I	TGTACA	2 cut(s)	285, 558
Bsp143I	GATC	2 cut(s)	293, 432
Bsp19I	CCATGG	1 cut(s)	54
BspACI	CCGC	4 cut(s)	59, 84, 139, 326
BspANI	GGCC	5 cut(s)	173, 260, 398, 493, 836
BspCNI	CTCAG	2 cut(s)	682, 761
BspFNI	CGCG	2 cut(s)	328, 921
BspHI	TCATGA	1 cut(s)	24
BspLI	GGNNCC	3 cut(s)	78, 960, 1054
BspPI	GGATC	1 cut(s)	301
BspT107I	GGYRCC	1 cut(s)	76
BsrDI	GCAATG	1 cut(s)	48
BsrFI	RCCGGY	1 cut(s)	73
BsrGI	TGTACA	2 cut(s)	285, 558
BsrI	ACTGG	2 cut(s)	769, 799
BssAI	RCCGGY	1 cut(s)	73
BssECI	CCNNGG	6 cut(s)	54, 174, 488, 843, 873, 1036
BssMI	GATC	2 cut(s)	293, 432
BssNI	GRCGYC	1 cut(s)	240
BssT1I	CCWWGG	4 cut(s)	54, 488, 873, 1036
Bst2UI	CCWGG	2 cut(s)	845, 963
Bst4CI	ACNGT	7 cut(s)	232, 415, 422, 430, 646, 724, 931
Bst6I	CTCTTC	1 cut(s)	487
BstACI	GRCGYC	1 cut(s)	240
BstAUI	TGTACA	2 cut(s)	285, 558
BstBAI	YACGTR	1 cut(s)	105
BstC8I	GCNNGC	2 cut(s)	519, 569
BstDEI	CTNAG	2 cut(s)	690, 748
BstDSI	CCRYGG	1 cut(s)	54
BstF5I	GGATG	1 cut(s)	899
BstFNI	CGCG	2 cut(s)	328, 921
BstHHI	GCGC	1 cut(s)	517
BstKTI	GATC	2 cut(s)	296, 435
BstMAI	GTCTC	1 cut(s)	678
BstMBI	GATC	2 cut(s)	293, 432
BstMCI	CGRYCG	2 cut(s)	174, 261
BstMWI	GCNNNNNNNGC	3 cut(s)	50, 148, 833
BstNI	CCWGG	2 cut(s)	845, 963
BstNSI	RCATGY	1 cut(s)	701
BstSCI	CCNGG	3 cut(s)	260, 843, 961
BstSFI	CTRYAG	1 cut(s)	426
BstUI	CGCG	2 cut(s)	328, 921
BstV1I	GCAGC	4 cut(s)	26, 445, 563, 1050
BstXI	CCANNNNNNTGG	2 cut(s)	55, 407
BstZI	CGGCCG	2 cut(s)	171, 258
BsuI	GTATCC	1 cut(s)	761
BsuRI	GGCC	5 cut(s)	173, 260, 398, 493, 836
BtgI	CCRYGG	1 cut(s)	54
BtsCI	GGATG	1 cut(s)	899
BtsI	GCAGTG	2 cut(s)	528, 781
BtsIMutI	CAGTG	7 cut(s)	427, 435, 528, 642, 687, 762, 781
Cac8I	GCNNGC	2 cut(s)	519, 569
CciI	TCATGA	1 cut(s)	24
CfoI	GCGC	1 cut(s)	517
Cfr10I	RCCGGY	1 cut(s)	73
Cfr13I	GGNCC	3 cut(s)	397, 834, 959
CseI	GACGC	1 cut(s)	248
Csp6I	GTAC	6 cut(s)	77, 130, 286, 304, 559, 946
CspAI	ACCGGT	1 cut(s)	73
CviAII	CATG	7 cut(s)	11, 25, 55, 202, 698, 868, 955
CviQI	GTAC	6 cut(s)	77, 130, 286, 304, 559, 946
DdeI	CTNAG	2 cut(s)	690, 748
DpnI	GATC	2 cut(s)	295, 434
DpnII	GATC	2 cut(s)	293, 432
EaeI	YGGCCR	2 cut(s)	171, 258
EagI	CGGCCG	2 cut(s)	171, 258
Eam1104I	CTCTTC	1 cut(s)	487
EarI	CTCTTC	1 cut(s)	487
EciI	GGCGGA	1 cut(s)	74
EclXI	CGGCCG	2 cut(s)	171, 258
Eco130I	CCWWGG	4 cut(s)	54, 488, 873, 1036
Eco47I	GGWCC	1 cut(s)	959
Eco52I	CGGCCG	2 cut(s)	171, 258
Eco72I	CACGTG	1 cut(s)	105
EcoRII	CCWGG	2 cut(s)	843, 961
EcoT14I	CCWWGG	4 cut(s)	54, 488, 873, 1036
EcoT22I	ATGCAT	1 cut(s)	859
ErhI	CCWWGG	4 cut(s)	54, 488, 873, 1036
FaeI	CATG	7 cut(s)	14, 28, 58, 205, 701, 871, 958
FaqI	GGGAC	2 cut(s)	758, 811
FatI	CATG	7 cut(s)	10, 24, 54, 201, 697, 867, 954
Fnu4HI	GCNGC	6 cut(s)	15, 84, 140, 459, 552, 1064
FokI	GGATG	1 cut(s)	906
Fsp4HI	GCNGC	6 cut(s)	15, 84, 140, 459, 552, 1064
GlaI	GCGC	1 cut(s)	516
GluI	GCNGC	6 cut(s)	15, 84, 140, 459, 552, 1064
GsaI	CCCAGC	1 cut(s)	707
HaeIII	GGCC	5 cut(s)	173, 260, 398, 493, 836
HapII	CCGG	4 cut(s)	74, 257, 261, 891
HgaI	GACGC	1 cut(s)	248
HhaI	GCGC	1 cut(s)	517
Hin1I	GRCGYC	1 cut(s)	240
Hin1II	CATG	7 cut(s)	14, 28, 58, 205, 701, 871, 958
Hin6I	GCGC	1 cut(s)	515
HinP1I	GCGC	1 cut(s)	515
HincII	GTYRAC	1 cut(s)	100
HindII	GTYRAC	1 cut(s)	100
HinfI	GANTC	4 cut(s)	322, 440, 746, 752
HpaII	CCGG	4 cut(s)	74, 257, 261, 891
HphI	GGTGA	3 cut(s)	226, 477, 736
Hpy166II	GTNNAC	6 cut(s)	100, 132, 286, 418, 781, 883
Hpy188I	TCNGA	4 cut(s)	445, 751, 757, 916
Hpy188III	TCNNGA	4 cut(s)	25, 226, 297, 584
Hpy8I	GTNNAC	6 cut(s)	100, 132, 286, 418, 781, 883
Hpy99I	CGWCG	1 cut(s)	242
HpyAV	CCTTC	2 cut(s)	632, 816
HpyCH4III	ACNGT	7 cut(s)	232, 415, 422, 430, 646, 724, 931
HpyCH4IV	ACGT	2 cut(s)	104, 274
HpyCH4V	TGCA	7 cut(s)	14, 205, 567, 697, 786, 857, 954
HpyF10VI	GCNNNNNNNGC	3 cut(s)	50, 148, 833
HpyF3I	CTNAG	2 cut(s)	690, 748
HpySE526I	ACGT	2 cut(s)	104, 274
Hsp92I	GRCGYC	1 cut(s)	240
Hsp92II	CATG	7 cut(s)	14, 28, 58, 205, 701, 871, 958
HspAI	GCGC	1 cut(s)	515
KpnI	GGTACC	1 cut(s)	80
Kzo9I	GATC	2 cut(s)	293, 432
LmnI	GCTCC	2 cut(s)	512, 897
Lsp1109I	GCAGC	4 cut(s)	26, 445, 563, 1050
MaeII	ACGT	2 cut(s)	104, 274
MaeIII	GTNAC	3 cut(s)	166, 232, 343
MalI	GATC	2 cut(s)	295, 434
MboI	GATC	2 cut(s)	293, 432
MboII	GAAGA	1 cut(s)	474
MfeI	CAATTG	1 cut(s)	562
MluCI	AATT	5 cut(s)	30, 315, 375, 562, 988
MlyI	GAGTC	1 cut(s)	740
MmeI	TCCRAC	1 cut(s)	386
MnlI	CCTC	7 cut(s)	47, 169, 525, 751, 825, 1080, 1083
Mph1103I	ATGCAT	1 cut(s)	859
MseI	TTAA	3 cut(s)	20, 246, 474
MslI	CAYNNNNRTG	2 cut(s)	53, 866
MspA1I	CMGCKG	1 cut(s)	139
MspI	CCGG	4 cut(s)	74, 257, 261, 891
MspR9I	CCNGG	3 cut(s)	262, 845, 963
MunI	CAATTG	1 cut(s)	562
Mva1269I	GAATGC	1 cut(s)	252
MvaI	CCWGG	2 cut(s)	845, 963
MvnI	CGCG	2 cut(s)	328, 921
MwoI	GCNNNNNNNGC	3 cut(s)	50, 148, 833
NciI	CCSGG	1 cut(s)	262
NcoI	CCATGG	1 cut(s)	54
NdeII	GATC	2 cut(s)	293, 432
NlaIII	CATG	7 cut(s)	14, 28, 58, 205, 701, 871, 958
NlaIV	GGNNCC	3 cut(s)	78, 960, 1054
NmeAIII	GCCGAG	1 cut(s)	199
NmuCI	GTSAC	3 cut(s)	166, 232, 343
NsiI	ATGCAT	1 cut(s)	859
NspI	RCATGY	1 cut(s)	701
PagI	TCATGA	1 cut(s)	24
PcsI	WCGNNNNNNNCGW	1 cut(s)	441
PctI	GAATGC	1 cut(s)	252
PfeI	GAWTC	3 cut(s)	322, 440, 752
PinAI	ACCGGT	1 cut(s)	73
PkrI	GCNGC	6 cut(s)	16, 85, 141, 460, 553, 1065
PleI	GAGTC	1 cut(s)	740
PmaCI	CACGTG	1 cut(s)	105
PmlI	CACGTG	1 cut(s)	105
PpsI	GAGTC	1 cut(s)	740
Ppu21I	YACGTR	1 cut(s)	105
PshBI	ATTAAT	1 cut(s)	246
Psp6I	CCWGG	2 cut(s)	843, 961
PspCI	CACGTG	1 cut(s)	105
PspFI	CCCAGC	1 cut(s)	703
PspGI	CCWGG	2 cut(s)	843, 961
PspN4I	GGNNCC	3 cut(s)	78, 960, 1054
PspPI	GGNCC	3 cut(s)	397, 834, 959
RsaI	GTAC	6 cut(s)	78, 131, 287, 305, 560, 947
RsaNI	GTAC	6 cut(s)	77, 130, 286, 304, 559, 946
RseI	CAYNNNNRTG	2 cut(s)	53, 866
SaqAI	TTAA	3 cut(s)	20, 246, 474
SatI	GCNGC	6 cut(s)	15, 84, 140, 459, 552, 1064
Sau3AI	GATC	2 cut(s)	293, 432
Sau96I	GGNCC	3 cut(s)	397, 834, 959
SchI	GAGTC	1 cut(s)	740
ScrFI	CCNGG	3 cut(s)	262, 845, 963
SetI	ASST	9 cut(s)	19, 107, 201, 277, 573, 849, 879, 967, 1005
SfcI	CTRYAG	1 cut(s)	426
SinI	GGWCC	1 cut(s)	959
SmiMI	CAYNNNNRTG	2 cut(s)	53, 866
Sse9I	AATT	5 cut(s)	30, 315, 375, 562, 988
SsiI	CCGC	4 cut(s)	59, 84, 139, 326
StyD4I	CCNGG	3 cut(s)	260, 843, 961
StyI	CCWWGG	4 cut(s)	54, 488, 873, 1036
TaaI	ACNGT	7 cut(s)	232, 415, 422, 430, 646, 724, 931
TaiI	ACGT	2 cut(s)	107, 277
TasI	AATT	5 cut(s)	30, 315, 375, 562, 988
TatI	WGTACW	4 cut(s)	285, 303, 558, 945
TauI	GCSGC	2 cut(s)	86, 142
TfiI	GAWTC	3 cut(s)	322, 440, 752
Tru1I	TTAA	3 cut(s)	20, 246, 474
Tru9I	TTAA	3 cut(s)	20, 246, 474
TscAI	CASTG	7 cut(s)	427, 435, 528, 649, 694, 769, 788
TseFI	GTSAC	3 cut(s)	166, 232, 343
TseI	GCWGC	4 cut(s)	14, 458, 551, 1063
Tsp45I	GTSAC	3 cut(s)	166, 232, 343
TspDTI	ATGAA	2 cut(s)	643, 1018
TspGWI	ACGGA	1 cut(s)	954
TspRI	CASTG	7 cut(s)	427, 435, 528, 649, 694, 769, 788
VpaK11BI	GGWCC	1 cut(s)	959
VspI	ATTAAT	1 cut(s)	246
XapI	RAATTY	1 cut(s)	988
XceI	RCATGY	1 cut(s)	701
XcmI	CCANNNNNNNNNTGG	2 cut(s)	52, 844
Zsp2I	ATGCAT	1 cut(s)	859

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.