RLG00000016600

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001024
Physical Location & Seq
Reverse (-)
8117114 .. 8119511
2398 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016600

Sequence Viewer

Length: 1131 bp
ATGGCTATGAGATCGACACCCGCTCTTGTGGTCGTTGCTGCATTTCTGGCTCTCCATTTCGCTCCTTTGCTCGTGTCGGGCCACCCCAACCTCATTCCGGAAAACGAGTCCGATCTCGATGGCTGGATCGTCCAAAACATGAGGGAGTACAACGAGCGGAAGGCCAAGCTGAGGACCAGGAGCGACCTCCCCGGCACTCACTTCGACACAGGGCTCGCCGCAGCCGAGGACGCAAAGAGAGTCATCCGGGTGAAGCAGGACGGCACCGGAGACTTTAGGACGGTGACGGACGCCGTGAGGAGCATTCCGTCTGCAAACAAGCAGCGCGTGGTGGTGTGGATTGGTGGGGGAGAGTACAGGGAGAAGGTTCTGGTGGATGTGACGAAGCCGTACGTGACGTTTTTTGGGGACAAGAACAACATCCCGTCGATCACCTACGACGGCACGGCTGCTGTGTACGGGACTTGGAACAGTGCCACGGTGGCCGTGGAGGCCGACTATTTCATGGCCGTGAATATAGCGTTCGTGAATTCAGCACCTATGCCGGACGGCAGAAGGAAAGGTGCACAAGCGGTGGCAATGAGAATATCAGGGGATAAGGCAGCCTTCCACAACTGCAGGTTCATCGGATTTCAGGACACCTTATGTGACGATAGGGGAAGGCATTTCTTCAAGGATTGCTTCATCCAAGGCACTGTTGATTTCATCTTTGGCAATGGAAAATCCCTCTACTTGAAAAGTACCTTAAACTCAGTCGCCAATGGAAATGGGGTGATCACTGCACATGGTAGAACAGATGTGCAGGATGATAGCGGATTTTCGTTTGTGTATTGCAACATAACTGGGACAGGTGAGACCTTGCTTGGGCGTGCTTGGAGGGGAAGAGCAAGAGTGGTGTTTTCTTACACATACATGGGGAACCTTGTCGACAGAGAAGGGTGGTCCGCCTACAACGACAGTGCGCGTGCCAACACTGTGTATTATGGAGAGTACAAATGTTCTGGACCGGGTTCAAGCTCCATCCATCGAGTCAAGTTTTCAAGGATGCTGACTGATATAGAAGCAAAACCCTTCATCAGCCTGACTTTCATCCAAGGAACGAAATGGGTTCTTCCCCCTCCCAACCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

377

Amino Acids

41.49

Weight (kDa)

8.96

Isoelectric Point (pI)

24.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 83 - 366 6.3e-70 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 609
AccB1I GGYRCC 1 cut(s) 263
AccBSI CCGCTC 2 cut(s) 23, 157
AccI GTMKAC 1 cut(s) 927
AccII CGCG 2 cut(s) 327, 964
AccIII TCCGGA 1 cut(s) 97
AciI CCGC 6 cut(s) 21, 157, 219, 572, 813, 945
AclWI GGATC 1 cut(s) 134
AcoI YGGCCR 2 cut(s) 483, 507
AcsI RAATTY 1 cut(s) 529
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 6 cut(s) 149, 356, 392, 458, 742, 992
AfiI CCNNNNNNNGG 3 cut(s) 97, 171, 864
AgsI TTSAA 4 cut(s) 673, 736, 1014, 1041
AjnI CCWGG 1 cut(s) 176
AluBI AGCT 2 cut(s) 169, 1017
AluI AGCT 2 cut(s) 169, 1017
Alw21I GWGCWC 1 cut(s) 568
Alw26I GTCTC 2 cut(s) 264, 848
Alw44I GTGCAC 1 cut(s) 564
AlwI GGATC 1 cut(s) 134
Aor13HI TCCGGA 1 cut(s) 97
AoxI GGCC 5 cut(s) 79, 162, 483, 492, 507
ApaLI GTGCAC 1 cut(s) 564
ApeKI GCWGC 5 cut(s) 38, 221, 322, 449, 602
ApoI RAATTY 1 cut(s) 529
AspLEI GCGC 2 cut(s) 327, 964
AspS9I GGNCC 4 cut(s) 79, 174, 942, 1004
AsuC2I CCSGG 3 cut(s) 192, 248, 1008
AsuHPI GGTGA 5 cut(s) 262, 295, 424, 784, 863
AvaII GGWCC 3 cut(s) 174, 942, 1004
BaeGI GKGCMC 1 cut(s) 568
BanI GGYRCC 1 cut(s) 263
BanII GRGCYC 1 cut(s) 216
BauI CACGAG 1 cut(s) 71
Bbv12I GWGCWC 1 cut(s) 568
BbvCI CCTCAGC 1 cut(s) 170
BbvI GCAGC 5 cut(s) 25, 233, 334, 436, 614
BccI CCATC 3 cut(s) 113, 1028, 1032
BceAI ACGGC 8 cut(s) 277, 278, 373, 457, 462, 470, 494, 565
BcgI CGANNNNNNTGC 4 cut(s) 184, 218, 607, 641
BciT130I CCWGG 1 cut(s) 178
BclI TGATCA 1 cut(s) 774
BcnI CCSGG 3 cut(s) 192, 248, 1008
BcoDI GTCTC 2 cut(s) 264, 848
BfaI CTAG 1 cut(s) 1129
BfmI CTRYAG 1 cut(s) 616
BfuAI ACCTGC 1 cut(s) 609
BglI GCCNNNNNGGC 2 cut(s) 482, 491
BisI GCNGC 6 cut(s) 39, 219, 222, 323, 450, 603
BlsI GCNGC 6 cut(s) 40, 220, 223, 324, 451, 604
Bme1390I CCNGG 4 cut(s) 178, 192, 248, 1008
Bme18I GGWCC 3 cut(s) 174, 942, 1004
BmgT120I GGNCC 4 cut(s) 79, 174, 942, 1004
BmiI GGNNCC 2 cut(s) 265, 920
BmrFI CCNGG 4 cut(s) 178, 192, 248, 1008
BmrI ACTGGG 1 cut(s) 852
BmsI GCATC 1 cut(s) 1035
BmuI ACTGGG 1 cut(s) 852
Bpu10I CCTNAGC 1 cut(s) 170
BpuMI CCSGG 3 cut(s) 192, 248, 1008
BsaAI YACGTR 1 cut(s) 394
BsaHI GRCGYC 1 cut(s) 291
BsaI GGTCTC 1 cut(s) 848
BsaJI CCNNGG 6 cut(s) 190, 225, 477, 486, 688, 1093
BsaWI WCCGGW 2 cut(s) 97, 266
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 171, 864
Bse1I ACTGG 1 cut(s) 847
Bse3DI GCAATG 2 cut(s) 585, 721
BseAI TCCGGA 1 cut(s) 97
BseBI CCWGG 1 cut(s) 178
BseDI CCNNGG 6 cut(s) 190, 225, 477, 486, 688, 1093
BseGI GGATG 8 cut(s) 243, 382, 420, 684, 811, 1020, 1050, 1089
BseLI CCNNNNNNNGG 3 cut(s) 97, 171, 864
BseMI GCAATG 2 cut(s) 585, 721
BseMII CTCAG 2 cut(s) 161, 765
BseNI ACTGG 1 cut(s) 847
BseRI GAGGAG 1 cut(s) 313
BseSI GKGCMC 1 cut(s) 568
BseXI GCAGC 5 cut(s) 25, 233, 334, 436, 614
BsgI GTGCAG 2 cut(s) 765, 821
Bsh1236I CGCG 2 cut(s) 327, 964
BshFI GGCC 5 cut(s) 81, 164, 485, 494, 509
BshNI GGYRCC 1 cut(s) 263
BsiHKAI GWGCWC 1 cut(s) 568
BsiSI CCGG 6 cut(s) 98, 192, 247, 267, 545, 1007
BsiWI CGTACG 1 cut(s) 390
BslFI GGGAC 3 cut(s) 422, 475, 859
BslI CCNNNNNNNGG 3 cut(s) 97, 171, 864
BsmAI GTCTC 2 cut(s) 264, 848
BsmFI GGGAC 3 cut(s) 422, 475, 859
BsmI GAATGC 1 cut(s) 303
BsnI GGCC 5 cut(s) 81, 164, 485, 494, 509
Bso31I GGTCTC 1 cut(s) 848
Bsp1286I GDGCHC 2 cut(s) 216, 568
Bsp13I TCCGGA 1 cut(s) 97
Bsp143I GATC 5 cut(s) 11, 112, 126, 429, 774
BspACI CCGC 6 cut(s) 21, 157, 219, 572, 813, 945
BspANI GGCC 5 cut(s) 81, 164, 485, 494, 509
BspCNI CTCAG 2 cut(s) 162, 764
BspEI TCCGGA 1 cut(s) 97
BspFNI CGCG 2 cut(s) 327, 964
BspLI GGNNCC 2 cut(s) 265, 920
BspMAI CTGCAG 1 cut(s) 620
BspMI ACCTGC 1 cut(s) 609
BspPI GGATC 1 cut(s) 134
BspQI GCTCTTC 1 cut(s) 877
BspT107I GGYRCC 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 848
BsrBI CCGCTC 2 cut(s) 23, 157
BsrDI GCAATG 2 cut(s) 585, 721
BsrI ACTGG 1 cut(s) 847
BssECI CCNNGG 6 cut(s) 190, 225, 477, 486, 688, 1093
BssMI GATC 5 cut(s) 11, 112, 126, 429, 774
BssNI GRCGYC 1 cut(s) 291
BssSI CACGAG 1 cut(s) 71
BssT1I CCWWGG 2 cut(s) 688, 1093
Bst2BI CACGAG 1 cut(s) 71
Bst2UI CCWGG 1 cut(s) 178
Bst4CI ACNGT 6 cut(s) 283, 473, 481, 697, 959, 976
Bst6I CTCTTC 1 cut(s) 877
BstACI GRCGYC 1 cut(s) 291
BstBAI YACGTR 1 cut(s) 394
BstC8I GCNNGC 3 cut(s) 216, 870, 966
BstDEI CTNAG 2 cut(s) 170, 751
BstDSI CCRYGG 2 cut(s) 477, 486
BstF5I GGATG 8 cut(s) 243, 382, 420, 684, 811, 1020, 1050, 1089
BstFNI CGCG 2 cut(s) 327, 964
BstHHI GCGC 2 cut(s) 327, 964
BstKTI GATC 5 cut(s) 14, 115, 129, 432, 777
BstMAI GTCTC 2 cut(s) 264, 848
BstMBI GATC 5 cut(s) 11, 112, 126, 429, 774
BstMWI GCNNNNNNNGC 4 cut(s) 47, 230, 482, 491
BstNI CCWGG 1 cut(s) 178
BstSCI CCNGG 4 cut(s) 176, 190, 246, 1006
BstSFI CTRYAG 1 cut(s) 616
BstSLI GKGCMC 1 cut(s) 568
BstUI CGCG 2 cut(s) 327, 964
BstV1I GCAGC 5 cut(s) 25, 233, 334, 436, 614
BsuRI GGCC 5 cut(s) 81, 164, 485, 494, 509
BtgI CCRYGG 2 cut(s) 477, 486
BtsCI GGATG 8 cut(s) 243, 382, 420, 684, 811, 1020, 1050, 1089
BtsI GCAGTG 1 cut(s) 777
BtsIMutI CAGTG 5 cut(s) 478, 693, 777, 964, 972
BveI ACCTGC 1 cut(s) 609
Cac8I GCNNGC 3 cut(s) 216, 870, 966
CfoI GCGC 2 cut(s) 327, 964
Cfr13I GGNCC 4 cut(s) 79, 174, 942, 1004
CseI GACGC 2 cut(s) 239, 299
Csp6I GTAC 6 cut(s) 148, 355, 391, 457, 741, 991
CviAII CATG 4 cut(s) 139, 505, 785, 913
CviQI GTAC 6 cut(s) 148, 355, 391, 457, 741, 991
DdeI CTNAG 2 cut(s) 170, 751
DpnI GATC 5 cut(s) 13, 114, 128, 431, 776
DpnII GATC 5 cut(s) 11, 112, 126, 429, 774
EaeI YGGCCR 2 cut(s) 483, 507
Eam1104I CTCTTC 1 cut(s) 877
EarI CTCTTC 1 cut(s) 877
EciI GGCGGA 1 cut(s) 934
Eco130I CCWWGG 2 cut(s) 688, 1093
Eco24I GRGCYC 1 cut(s) 216
Eco31I GGTCTC 1 cut(s) 848
Eco47I GGWCC 3 cut(s) 174, 942, 1004
EcoRI GAATTC 1 cut(s) 529
EcoRII CCWGG 1 cut(s) 176
EcoT14I CCWWGG 2 cut(s) 688, 1093
EcoT38I GRGCYC 1 cut(s) 216
ErhI CCWWGG 2 cut(s) 688, 1093
FaeI CATG 4 cut(s) 142, 508, 788, 916
FalI AAGNNNNNCTT 4 cut(s) 590, 622, 665, 697
FaqI GGGAC 3 cut(s) 422, 475, 859
FatI CATG 4 cut(s) 138, 504, 784, 912
FauI CCCGC 1 cut(s) 28
FbaI TGATCA 1 cut(s) 774
FblI GTMKAC 1 cut(s) 927
Fnu4HI GCNGC 6 cut(s) 39, 219, 222, 323, 450, 603
FokI GGATG 8 cut(s) 230, 389, 407, 671, 818, 1007, 1057, 1076
FriOI GRGCYC 1 cut(s) 216
Fsp4HI GCNGC 6 cut(s) 39, 219, 222, 323, 450, 603
FspBI CTAG 1 cut(s) 1129
GlaI GCGC 2 cut(s) 326, 963
GluI GCNGC 6 cut(s) 39, 219, 222, 323, 450, 603
HaeIII GGCC 5 cut(s) 81, 164, 485, 494, 509
HapII CCGG 6 cut(s) 98, 192, 247, 267, 545, 1007
HgaI GACGC 2 cut(s) 239, 299
HhaI GCGC 2 cut(s) 327, 964
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 4 cut(s) 142, 508, 788, 916
Hin6I GCGC 2 cut(s) 325, 962
HinP1I GCGC 2 cut(s) 325, 962
HincII GTYRAC 1 cut(s) 928
HindII GTYRAC 1 cut(s) 928
HinfI GANTC 3 cut(s) 107, 240, 1029
HpaII CCGG 6 cut(s) 98, 192, 247, 267, 545, 1007
HphI GGTGA 5 cut(s) 262, 295, 424, 784, 863
Hpy166II GTNNAC 3 cut(s) 457, 566, 928
Hpy188I TCNGA 2 cut(s) 112, 629
Hpy188III TCNNGA 5 cut(s) 98, 116, 526, 635, 1002
Hpy8I GTNNAC 3 cut(s) 457, 566, 928
Hpy99I CGWCG 2 cut(s) 430, 443
HpyAV CCTTC 7 cut(s) 154, 358, 549, 616, 654, 929, 1081
HpyCH4III ACNGT 6 cut(s) 283, 473, 481, 697, 959, 976
HpyCH4IV ACGT 2 cut(s) 393, 398
HpyCH4V TGCA 7 cut(s) 41, 314, 566, 618, 782, 802, 834
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 230, 482, 491
HpyF3I CTNAG 2 cut(s) 170, 751
HpySE526I ACGT 2 cut(s) 393, 398
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 4 cut(s) 142, 508, 788, 916
HspAI GCGC 2 cut(s) 325, 962
Kpn2I TCCGGA 1 cut(s) 97
Ksp22I TGATCA 1 cut(s) 774
Kzo9I GATC 5 cut(s) 11, 112, 126, 429, 774
LguI GCTCTTC 1 cut(s) 877
LmnI GCTCC 4 cut(s) 67, 180, 300, 1022
Lsp1109I GCAGC 5 cut(s) 25, 233, 334, 436, 614
LweI GCATC 1 cut(s) 1035
MaeI CTAG 1 cut(s) 1129
MaeII ACGT 2 cut(s) 393, 398
MaeIII GTNAC 4 cut(s) 283, 379, 394, 647
MalI GATC 5 cut(s) 13, 114, 128, 431, 776
MbiI CCGCTC 2 cut(s) 23, 157
MboI GATC 5 cut(s) 11, 112, 126, 429, 774
MboII GAAGA 3 cut(s) 661, 894, 1103
MhlI GDGCHC 2 cut(s) 216, 568
MluCI AATT 1 cut(s) 529
MlyI GAGTC 3 cut(s) 116, 249, 1038
MroI TCCGGA 1 cut(s) 97
MseI TTAA 1 cut(s) 746
MslI CAYNNNNRTG 3 cut(s) 248, 509, 911
MspI CCGG 6 cut(s) 98, 192, 247, 267, 545, 1007
MspR9I CCNGG 4 cut(s) 178, 192, 248, 1008
Mva1269I GAATGC 1 cut(s) 303
MvaI CCWGG 1 cut(s) 178
MvnI CGCG 2 cut(s) 327, 964
MwoI GCNNNNNNNGC 4 cut(s) 47, 230, 482, 491
NciI CCSGG 3 cut(s) 192, 248, 1008
NdeII GATC 5 cut(s) 11, 112, 126, 429, 774
NlaIII CATG 4 cut(s) 142, 508, 788, 916
NlaIV GGNNCC 2 cut(s) 265, 920
NmeAIII GCCGAG 1 cut(s) 250
NmuCI GTSAC 4 cut(s) 283, 379, 394, 647
PciSI GCTCTTC 1 cut(s) 877
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PctI GAATGC 1 cut(s) 303
Pfl23II CGTACG 1 cut(s) 390
PkrI GCNGC 6 cut(s) 40, 220, 223, 324, 451, 604
PleI GAGTC 3 cut(s) 115, 248, 1037
PpsI GAGTC 3 cut(s) 115, 248, 1037
Ppu21I YACGTR 1 cut(s) 394
Psp6I CCWGG 1 cut(s) 176
PspGI CCWGG 1 cut(s) 176
PspLI CGTACG 1 cut(s) 390
PspN4I GGNNCC 2 cut(s) 265, 920
PspPI GGNCC 4 cut(s) 79, 174, 942, 1004
PstI CTGCAG 1 cut(s) 620
RsaI GTAC 6 cut(s) 149, 356, 392, 458, 742, 992
RsaNI GTAC 6 cut(s) 148, 355, 391, 457, 741, 991
RseI CAYNNNNRTG 3 cut(s) 248, 509, 911
SalI GTCGAC 1 cut(s) 926
SapI GCTCTTC 1 cut(s) 877
SaqAI TTAA 1 cut(s) 746
SatI GCNGC 6 cut(s) 39, 219, 222, 323, 450, 603
Sau3AI GATC 5 cut(s) 11, 112, 126, 429, 774
Sau96I GGNCC 4 cut(s) 79, 174, 942, 1004
SchI GAGTC 3 cut(s) 116, 249, 1038
ScrFI CCNGG 4 cut(s) 178, 192, 248, 1008
SduI GDGCHC 2 cut(s) 216, 568
SfaNI GCATC 1 cut(s) 1035
SfcI CTRYAG 1 cut(s) 616
SfiI GGCCNNNNNGGCC 1 cut(s) 491
SinI GGWCC 3 cut(s) 174, 942, 1004
SmiMI CAYNNNNRTG 3 cut(s) 248, 509, 911
Sse9I AATT 1 cut(s) 529
SsiI CCGC 6 cut(s) 21, 157, 219, 572, 813, 945
SspMI CTAG 1 cut(s) 1129
StyD4I CCNGG 4 cut(s) 176, 190, 246, 1006
StyI CCWWGG 2 cut(s) 688, 1093
TaaI ACNGT 6 cut(s) 283, 473, 481, 697, 959, 976
TaiI ACGT 2 cut(s) 396, 401
TaqI TCGA 6 cut(s) 14, 117, 204, 428, 927, 1027
TasI AATT 1 cut(s) 529
TatI WGTACW 3 cut(s) 147, 354, 990
TauI GCSGC 1 cut(s) 221
Tru1I TTAA 1 cut(s) 746
Tru9I TTAA 1 cut(s) 746
TscAI CASTG 5 cut(s) 478, 700, 784, 964, 979
TseFI GTSAC 4 cut(s) 283, 379, 394, 647
TseI GCWGC 5 cut(s) 38, 221, 322, 449, 602
Tsp45I GTSAC 4 cut(s) 283, 379, 394, 647
TspDTI ATGAA 6 cut(s) 493, 613, 673, 694, 1063, 1078
TspGWI ACGGA 2 cut(s) 297, 302
TspRI CASTG 5 cut(s) 478, 700, 784, 964, 979
VneI GTGCAC 1 cut(s) 564
VpaK11BI GGWCC 3 cut(s) 174, 942, 1004
XapI RAATTY 1 cut(s) 529
XcmI CCANNNNNNNNNTGG 1 cut(s) 484
XmiI GTMKAC 1 cut(s) 927
XspI CTAG 1 cut(s) 1129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.