Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0000828

Physical Location & Seq

Genomic Coordinates Reverse (-)

3897893 .. 3901790

Gene Structure 3898 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G12080.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2205 bp

ATGGCAGAACAAAAGAGTAGTAACGGAGGAGGAGGAGGAGGAGATGTTGTTATCAATGTTCCAGTTGAGGAAGCATCAAGGCGTTCCAAGGAAATGGCTTCACCAGAGTCTGAGAAAGGAGTTCCCTTTAGTAAAAGCCCTTCTCCTGAAATCTCTAAGCTTGTTGGTAGTCCTAACAAGCCTCCTAGAGCTCCAAATCAGAACAATGTGGGTCTAACTCAGAGGAAATCTTTTGCAAGGTCGGTTTACTCAAAACCCAAGTCCCGGTTTGTTGATCCATCTTGTCCTGTAGACACAAGTATTCTAGAGGAGGAAGTTAGGGAGCAACTTGGTGCTGGTTTTTCTTTTAGTAGAGCTTCTCCGAATAACAAATCTAATAGGAGTGTCGGGTCACCAGCACCGGTTACTCCAAGTAAAGTCGTTGTTGAGAAAGATGAGGATGAGGAAATCTACAAGAAGGTTAAGCTGAACAGAGAGATGCGCAGTAAGATAAGTACATTGGCTTTGATAGAGTCAGCTTTCTTTGTGGTGATTTTGAGCGCTTTGGTTGCGAGTTTAACCATTAATGTCCTGAAACATCACACCTTCTGGGGGCTAGAAGTCTGGAAATGGTGTGTGCTTGTGATGGTTATATTCAGTGGAATGTTGGTGACAAACTGGTTCATGCGTTTGATTGTGTTCCTCATAGAAACAAACTTTCTTTTGAGGAGAAAAGTGCTCTACTTTGTGCACGGCTTGAAGAAGAGCGTCCAAGTTTTCATTTGGCTCTGCTTGATTCTTGTTGCTTGGATATTGTTGTTCAACCACGACGTGAAACGGTCCCCCGCAGCCACCAAAGTCCTCAAATGTATTACCAGGACTCTTATTTCCATTCTTACAGGGGCATTCTTTTGGCTGGTGAAAACACTCTTGTTGAAAATCCTTGCAGCGAATTTCAACGTCAATAACTTTTTCGATAGGATTCAAGATTCTGTTTTCCACCAGTATGTTCTACAAACGCTCTCGGGTCTTCCACTTATGGAAGAGGCAGAGAGGGTCGGGCGTGAGCCAAGCACAGGCCATTTGAGTTTCGCGACTGTAGTGAAAAAAGGAACGGTTAAAGAGAAGAAAGTGATTGATATGGGGAAAGTTCATAAGATGAAGCGGGAGAAAGTTTCGGCTTGGACTATGCGAGTTTTGATGGAAGCGGTTAGAACTTCAGGTCTCTCTACTATCTCTGACACATTGGACGAAACAGCATACGGCGAGGGGAAAGAGCAAGCTGACAGAGAAATTACTAGTGAGATGGAGGCTTTGGCTGCTGCTTACCATGTCTTCAGAAATGTTGCTCAGCCCTTCTTCAATTACATAGAGGAAGAGGACTTGCTTAGGTTTATGATTAAGGAAGAGGTTGATCTTGTGTTCCCATTGTTTGATGGTGCCGCTGAGACCGGGAGAATTACAAGAAAAGCTTTCACAGAATGGGTGGTTAAGGTGTACACGAGCCGGAGAGCTTTAGCGCATTCCTTAAACGACACAAAAACAGCGGTTAAGCAGTTAAACAAACTTGTGACAGCAATCTTGATGGTGGTTACCGTTGTCATTTGGCTGCTCCTTCTAGAAGTAGCAACGACTAAGGTTTTGCTGTTCTTCTCCACCCAACTCGTGGCTCTGGCTTTTATAATCGGAAGCACATGCAAAAACCTCTTTGAATCCATTGTGTTCGTATTCGTCATGCATCCTTATGATGTCGGTGATCGATGTGTTGTTGACGGTGTCGCGATGCTGGTGGAAGAAATGAATCTCTTAACGACAGTGTTCTTGAAGCTTAACAACGAGAAAGTGTATTATCCGAACGCTGTTTTGGCCACGAAACCGATAAGCAATTACTTCAGAAGTCCGAATATGGGAGAAACAGTGGAATTCTCTATCTCTTTCTCGACACCAGTCTCTAAGATAGCACATCTCAAAGAAAGAATCGCCGAGTACTTGGAGCAGAACCCGCAACATTGGGCACCGGTTCACTCGGTGGTGGTGAAGGAGATAGAGAACATGAACAAGCTGAAGATGGCCCTATACAGTGACCACACCATCACGTTTCAGGAAAACAGAGAGAGGAATCTTAGAAGAACCGAACTTTCTTTGGCCATTAAGAGAATGTTGGAGGACCTTCACATCGACTACACTCTCCTTCCTCAAGACATTAATCTCACAAAGAAGAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0080167 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

734

Amino Acids

83.03

Weight (kDa)

9.19

Isoelectric Point (pI)

42.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Msy1	PF25886	174 - 312	9.5e-07	Mechanosensitive ion channel protein Msy1-like, transmembrane domain
MS_channel_2nd	PF00924	559 - 623	9.7e-09	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000828)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12080 AT5G12080 AT5G12080 AT5G19520 AT5G19520
fragaria_vesca	FvH4_2g31211 FvH4_2g31220 FvH4_2g31230 FvH4_2g31240
malus_domestica	MD08G1070000.v1.1 MD15G1052100.v1.1
prunus_persica	Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1
pyrus_communis	pycom08g05800 pycom15g04990 pycom16g12570
rosa_chinensis	RchiOBHm_Chr1g0326931 RchiOBHm_Chr1g0326941 RchiOBHm_Chr6g0276141 RchiOBHm_Chr6g0283901 RchiOBHm_Chr6g0311561 RchiOBHm_Chr6g0311571 RchiOBHm_Chr6g0311581 RchiOBHm_Chr6g0311591
rosa_laevigata	RLG00000010389 RLG00000010390 RLG00000010391 RLG00000010393 RLG00000012788 RLG00000030050
rosa_multiflora	Rmu_sc0003315.1_g000003 Rmu_sc0006781.1_g000009 Rmu_sc0007556.1_g000006 Rmu_sc0018059.1_g000002 Rmu_sc0018059.1_g000003
rosa_roxburghii	Rroxscaffold_4G00323490 Rroxscaffold_7G00157470 Rroxscaffold_7G00157480 Rroxscaffold_7G00157490 Rroxscaffold_7G00185080
rosa_rugosa	Rorug01G0062100 Rorug01G0062300 Rorug06G0158200 Rorug06G0158300 Rorug06G0389800.1 Rorug06G0389900
rosa_samantha	Rh1AG079400 Rh1BG063600 Rh1CG077000 Rh1DG083500 Rh6AG504700 Rh6AG504800 Rh6AG504900 Rh6BG272400 Rh6BG515600 Rh6BG515700 Rh6BG515800 Rh6CG521400 Rh6CG521600 Rh6DG266500 Rh6DG507300 Rh6DG507400
rosa_wichuraiana	Rw1G006300 Rw6G018310 Rw6G023400 Rw6G043960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1661
Acc16I	TGCGCA	1 cut(s)	482
AccB1I	GGYRCC	2 cut(s)	1418, 1993
AccB7I	CCANNNNNTGG	1 cut(s)	1645
AccI	GTMKAC	1 cut(s)	291
AccII	CGCG	2 cut(s)	1073, 1760
AciI	CCGC	6 cut(s)	825, 1144, 1187, 1422, 1526, 1982
AclWI	GGATC	1 cut(s)	269
AcoI	YGGCCR	2 cut(s)	1845, 2124
AcsI	RAATTY	2 cut(s)	931, 1901
AcuI	CTGAAG	4 cut(s)	1182, 1300, 1855, 2063
AdeI	CACNNNGTG	2 cut(s)	811, 2008
AfaI	GTAC	3 cut(s)	496, 1478, 1967
AfeI	AGCGCT	1 cut(s)	541
AfiI	CCNNNNNNNGG	5 cut(s)	264, 591, 1055, 1645, 1886
AgeI	ACCGGT	2 cut(s)	400, 1996
AgsI	TTSAA	8 cut(s)	739, 802, 916, 937, 965, 1342, 1691, 1804
AhlI	ACTAGT	1 cut(s)	1277
AjiI	CACGTC	1 cut(s)	811
AjnI	CCWGG	1 cut(s)	854
AjuI	GAANNNNNNNTTGG	2 cut(s)	1826, 1858
Alw21I	GWGCWC	3 cut(s)	193, 720, 732
Alw26I	GTCTC	3 cut(s)	1208, 1421, 1933
Alw44I	GTGCAC	1 cut(s)	728
AlwI	GGATC	1 cut(s)	269
AlwNI	CAGNNNCTG	1 cut(s)	110
Ama87I	CYCGRG	1 cut(s)	1003
Aor51HI	AGCGCT	1 cut(s)	541
AoxI	GGCC	4 cut(s)	1057, 1845, 2049, 2124
ApaLI	GTGCAC	1 cut(s)	728
ApeKI	GCWGC	5 cut(s)	827, 926, 1298, 1301, 1588
ApoI	RAATTY	2 cut(s)	931, 1901
ArsI	GACNNNNNNTTYG	4 cut(s)	245, 277, 1603, 1635
AseI	ATTAAT	2 cut(s)	564, 2184
AsiGI	ACCGGT	2 cut(s)	400, 1996
AspLEI	GCGC	3 cut(s)	483, 542, 1501
AspS9I	GGNCC	3 cut(s)	819, 2050, 2146
AsuC2I	CCSGG	2 cut(s)	265, 1432
AsuHPI	GGTGA	7 cut(s)	93, 384, 541, 661, 910, 1745, 2026
AvaI	CYCGRG	1 cut(s)	1003
AvaII	GGWCC	2 cut(s)	819, 2146
BaeGI	GKGCMC	2 cut(s)	732, 1996
BalI	TGGCCA	2 cut(s)	1847, 2126
BanI	GGYRCC	2 cut(s)	1418, 1993
BanII	GRGCYC	1 cut(s)	193
BarI	GAAGNNNNNNTAC	2 cut(s)	124, 156
BauI	CACGAG	2 cut(s)	1480, 1643
BbsI	GAAGAC	2 cut(s)	1001, 1306
Bbv12I	GWGCWC	3 cut(s)	193, 720, 732
BbvI	GCAGC	5 cut(s)	839, 938, 1285, 1288, 1575
BccI	CCATC	8 cut(s)	286, 619, 1174, 1279, 1409, 1558, 2041, 2078
BceAI	ACGGC	2 cut(s)	748, 1258
BciT130I	CCWGG	1 cut(s)	856
BcnI	CCSGG	2 cut(s)	265, 1432
BcoDI	GTCTC	3 cut(s)	1208, 1421, 1933
BcuI	ACTAGT	1 cut(s)	1277
BfaI	CTAG	5 cut(s)	186, 305, 596, 1278, 1598
BfmI	CTRYAG	2 cut(s)	288, 1077
BfoI	RGCGCY	1 cut(s)	543
BisI	GCNGC	6 cut(s)	828, 927, 1299, 1302, 1422, 1589
BlpI	GCTNAGC	1 cut(s)	1329
BlsI	GCNGC	6 cut(s)	829, 928, 1300, 1303, 1423, 1590
BmcAI	AGTACT	1 cut(s)	1967
Bme1390I	CCNGG	3 cut(s)	265, 856, 1432
Bme18I	GGWCC	2 cut(s)	819, 2146
BmeT110I	CYCGRG	1 cut(s)	1003
BmgBI	CACGTC	1 cut(s)	811
BmgT120I	GGNCC	3 cut(s)	819, 2050, 2146
BmiI	GGNNCC	3 cut(s)	821, 1420, 1995
BmrFI	CCNGG	3 cut(s)	265, 856, 1432
BmsI	GCATC	4 cut(s)	83, 468, 1726, 1752
BoxI	GACNNNNGTC	1 cut(s)	1925
BpiI	GAAGAC	2 cut(s)	1001, 1306
Bpu10I	CCTNAGC	1 cut(s)	1367
Bpu1102I	GCTNAGC	1 cut(s)	1329
BpuEI	CTTGAG	1 cut(s)	2160
BpuMI	CCSGG	2 cut(s)	265, 1432
Bsa29I	ATCGAT	1 cut(s)	1738
BsaI	GGTCTC	2 cut(s)	1208, 1421
BsaJI	CCNNGG	1 cut(s)	87
BsaWI	WCCGGW	2 cut(s)	400, 1996
BsaXI	ACNNNNNCTCC	6 cut(s)	30, 33, 60, 63, 1881, 1911
Bsc4I	CCNNNNNNNGG	5 cut(s)	264, 591, 1055, 1645, 1886
Bse118I	RCCGGY	2 cut(s)	400, 1996
Bse1I	ACTGG	4 cut(s)	62, 662, 982, 1925
BseBI	CCWGG	1 cut(s)	856
BseCI	ATCGAT	1 cut(s)	1738
BseDI	CCNNGG	1 cut(s)	87
BseGI	GGATG	2 cut(s)	445, 1717
BseLI	CCNNNNNNNGG	5 cut(s)	264, 591, 1055, 1645, 1886
BseMII	CTCAG	4 cut(s)	102, 233, 1343, 1416
BseNI	ACTGG	4 cut(s)	62, 662, 982, 1925
BseRI	GAGGAG	7 cut(s)	42, 45, 48, 51, 54, 323, 721
BseSI	GKGCMC	2 cut(s)	732, 1996
BseXI	GCAGC	5 cut(s)	839, 938, 1285, 1288, 1575
Bsh1236I	CGCG	2 cut(s)	1073, 1760
BshFI	GGCC	4 cut(s)	1059, 1847, 2051, 2126
BshNI	GGYRCC	2 cut(s)	1418, 1993
BshTI	ACCGGT	2 cut(s)	400, 1996
BshVI	ATCGAT	1 cut(s)	1738
BsiHKAI	GWGCWC	3 cut(s)	193, 720, 732
BsiHKCI	CYCGRG	1 cut(s)	1003
BsiSI	CCGG	5 cut(s)	265, 401, 1431, 1486, 1997
BslFI	GGGAC	2 cut(s)	247, 805
BslI	CCNNNNNNNGG	5 cut(s)	264, 591, 1055, 1645, 1886
BsmAI	GTCTC	3 cut(s)	1208, 1421, 1933
BsmFI	GGGAC	2 cut(s)	247, 805
BsmI	GAATGC	2 cut(s)	884, 1501
BsnI	GGCC	4 cut(s)	1059, 1847, 2051, 2126
Bso31I	GGTCTC	2 cut(s)	1208, 1421
BsoBI	CYCGRG	1 cut(s)	1003
Bsp1286I	GDGCHC	4 cut(s)	193, 720, 732, 1996
Bsp1407I	TGTACA	1 cut(s)	1476
Bsp143I	GATC	3 cut(s)	274, 1393, 1735
Bsp1720I	GCTNAGC	1 cut(s)	1329
Bsp68I	TCGCGA	2 cut(s)	1073, 1760
BspACI	CCGC	6 cut(s)	825, 1144, 1187, 1422, 1526, 1982
BspANI	GGCC	4 cut(s)	1059, 1847, 2051, 2126
BspCNI	CTCAG	4 cut(s)	103, 232, 1342, 1417
BspDI	ATCGAT	1 cut(s)	1738
BspFNI	CGCG	2 cut(s)	1073, 1760
BspLI	GGNNCC	3 cut(s)	821, 1420, 1995
BspPI	GGATC	1 cut(s)	269
BspQI	GCTCTTC	1 cut(s)	737
BspT107I	GGYRCC	2 cut(s)	1418, 1993
BspTNI	GGTCTC	2 cut(s)	1208, 1421
BsrFI	RCCGGY	2 cut(s)	400, 1996
BsrGI	TGTACA	1 cut(s)	1476
BsrI	ACTGG	4 cut(s)	62, 662, 982, 1925
BssAI	RCCGGY	2 cut(s)	400, 1996
BssECI	CCNNGG	1 cut(s)	87
BssMI	GATC	3 cut(s)	274, 1393, 1735
BssSI	CACGAG	2 cut(s)	1480, 1643
BssT1I	CCWWGG	1 cut(s)	87
Bst2BI	CACGAG	2 cut(s)	1480, 1643
Bst2UI	CCWGG	1 cut(s)	856
Bst4CI	ACNGT	8 cut(s)	819, 1078, 1096, 1576, 1754, 1795, 1897, 2060
Bst6I	CTCTTC	4 cut(s)	737, 1017, 1350, 1380
BstAUI	TGTACA	1 cut(s)	1476
BstC8I	GCNNGC	1 cut(s)	1260
BstDEI	CTNAG	9 cut(s)	111, 156, 219, 1329, 1367, 1425, 1614, 1932, 2102
BstEII	GGTNACC	2 cut(s)	390, 1570
BstF5I	GGATG	2 cut(s)	445, 1717
BstFNI	CGCG	2 cut(s)	1073, 1760
BstH2I	RGCGCY	1 cut(s)	543
BstHHI	GCGC	3 cut(s)	483, 542, 1501
BstKTI	GATC	3 cut(s)	277, 1396, 1738
BstMAI	GTCTC	3 cut(s)	1208, 1421, 1933
BstMBI	GATC	3 cut(s)	274, 1393, 1735
BstMWI	GCNNNNNNNGC	4 cut(s)	548, 1298, 1844, 1981
BstNI	CCWGG	1 cut(s)	856
BstNSI	RCATGY	1 cut(s)	1677
BstPAI	GACNNNNGTC	1 cut(s)	1925
BstPI	GGTNACC	2 cut(s)	390, 1570
BstSCI	CCNGG	3 cut(s)	263, 854, 1430
BstSFI	CTRYAG	2 cut(s)	288, 1077
BstSLI	GKGCMC	2 cut(s)	732, 1996
BstUI	CGCG	2 cut(s)	1073, 1760
BstV1I	GCAGC	5 cut(s)	839, 938, 1285, 1288, 1575
BstV2I	GAAGAC	2 cut(s)	1001, 1306
BstXI	CCANNNNNNTGG	1 cut(s)	94
Bsu15I	ATCGAT	1 cut(s)	1738
BsuRI	GGCC	4 cut(s)	1059, 1847, 2051, 2126
BsuTUI	ATCGAT	1 cut(s)	1738
BtgZI	GCGATG	1 cut(s)	1775
BtrI	CACGTC	1 cut(s)	811
BtsCI	GGATG	2 cut(s)	445, 1717
BtsIMutI	CAGTG	4 cut(s)	643, 1800, 1902, 2065
BtuMI	TCGCGA	2 cut(s)	1073, 1760
Cac8I	GCNNGC	1 cut(s)	1260
CaiI	CAGNNNCTG	1 cut(s)	110
CfoI	GCGC	3 cut(s)	483, 542, 1501
Cfr10I	RCCGGY	2 cut(s)	400, 1996
Cfr13I	GGNCC	3 cut(s)	819, 2050, 2146
ClaI	ATCGAT	1 cut(s)	1738
CseI	GACGC	1 cut(s)	736
Csp6I	GTAC	3 cut(s)	495, 1477, 1966
CspAI	ACCGGT	2 cut(s)	400, 1996
CviAII	CATG	5 cut(s)	664, 1310, 1674, 1714, 2032
CviQI	GTAC	3 cut(s)	495, 1477, 1966
DdeI	CTNAG	9 cut(s)	111, 156, 219, 1329, 1367, 1425, 1614, 1932, 2102
DpnI	GATC	3 cut(s)	276, 1395, 1737
DpnII	GATC	3 cut(s)	274, 1393, 1735
DraIII	CACNNNGTG	2 cut(s)	811, 2008
EaeI	YGGCCR	2 cut(s)	1845, 2124
Eam1104I	CTCTTC	4 cut(s)	737, 1017, 1350, 1380
EarI	CTCTTC	4 cut(s)	737, 1017, 1350, 1380
Ecl136II	GAGCTC	1 cut(s)	191
Eco130I	CCWWGG	1 cut(s)	87
Eco24I	GRGCYC	1 cut(s)	193
Eco31I	GGTCTC	2 cut(s)	1208, 1421
Eco47I	GGWCC	2 cut(s)	819, 2146
Eco47III	AGCGCT	1 cut(s)	541
Eco53kI	GAGCTC	1 cut(s)	191
Eco57I	CTGAAG	4 cut(s)	1182, 1300, 1855, 2063
Eco88I	CYCGRG	1 cut(s)	1003
Eco91I	GGTNACC	2 cut(s)	390, 1570
EcoICRI	GAGCTC	1 cut(s)	191
EcoO109I	RGGNCCY	1 cut(s)	2146
EcoO65I	GGTNACC	2 cut(s)	390, 1570
EcoRI	GAATTC	1 cut(s)	1901
EcoRII	CCWGG	1 cut(s)	854
EcoT14I	CCWWGG	1 cut(s)	87
EcoT22I	ATGCAT	1 cut(s)	1719
EcoT38I	GRGCYC	1 cut(s)	193
ErhI	CCWWGG	1 cut(s)	87
FaeI	CATG	5 cut(s)	667, 1313, 1677, 1717, 2035
FalI	AAGNNNNNCTT	2 cut(s)	1435, 1467
FaqI	GGGAC	2 cut(s)	247, 805
FatI	CATG	5 cut(s)	663, 1309, 1673, 1713, 2031
FauI	CCCGC	3 cut(s)	832, 1137, 1989
FblI	GTMKAC	1 cut(s)	291
Fnu4HI	GCNGC	6 cut(s)	828, 927, 1299, 1302, 1422, 1589
FokI	GGATG	2 cut(s)	452, 1704
FriOI	GRGCYC	1 cut(s)	193
Fsp4HI	GCNGC	6 cut(s)	828, 927, 1299, 1302, 1422, 1589
FspBI	CTAG	5 cut(s)	186, 305, 596, 1278, 1598
FspI	TGCGCA	1 cut(s)	482
GlaI	GCGC	3 cut(s)	482, 541, 1500
GluI	GCNGC	6 cut(s)	828, 927, 1299, 1302, 1422, 1589
HaeII	RGCGCY	1 cut(s)	543
HaeIII	GGCC	4 cut(s)	1059, 1847, 2051, 2126
HapII	CCGG	5 cut(s)	265, 401, 1431, 1486, 1997
HgaI	GACGC	1 cut(s)	736
HhaI	GCGC	3 cut(s)	483, 542, 1501
Hin1II	CATG	5 cut(s)	667, 1313, 1677, 1717, 2035
Hin6I	GCGC	3 cut(s)	481, 540, 1499
HinP1I	GCGC	3 cut(s)	481, 540, 1499
HincII	GTYRAC	1 cut(s)	1750
HindII	GTYRAC	1 cut(s)	1750
HindIII	AAGCTT	3 cut(s)	158, 1449, 1805
HpaII	CCGG	5 cut(s)	265, 401, 1431, 1486, 1997
HphI	GGTGA	7 cut(s)	93, 384, 541, 661, 910, 1745, 2026
Hpy166II	GTNNAC	7 cut(s)	247, 292, 730, 1477, 1479, 1750, 2002
Hpy8I	GTNNAC	7 cut(s)	247, 292, 730, 1477, 1479, 1750, 2002
Hpy99I	CGWCG	1 cut(s)	812
HpyAV	CCTTC	8 cut(s)	150, 451, 595, 1345, 1604, 2011, 2159, 2180
HpyCH4III	ACNGT	8 cut(s)	819, 1078, 1096, 1576, 1754, 1795, 1897, 2060
HpyCH4IV	ACGT	3 cut(s)	810, 939, 2075
HpyCH4V	TGCA	5 cut(s)	236, 730, 926, 1677, 1717
HpyF10VI	GCNNNNNNNGC	4 cut(s)	548, 1298, 1844, 1981
HpyF3I	CTNAG	9 cut(s)	111, 156, 219, 1329, 1367, 1425, 1614, 1932, 2102
HpySE526I	ACGT	3 cut(s)	810, 939, 2075
Hsp92II	CATG	5 cut(s)	667, 1313, 1677, 1717, 2035
HspAI	GCGC	3 cut(s)	481, 540, 1499
Kzo9I	GATC	3 cut(s)	274, 1393, 1735
LguI	GCTCTTC	1 cut(s)	737
LmnI	GCTCC	4 cut(s)	196, 322, 1596, 1972
Lsp1109I	GCAGC	5 cut(s)	839, 938, 1285, 1288, 1575
LweI	GCATC	4 cut(s)	83, 468, 1726, 1752
MaeI	CTAG	5 cut(s)	186, 305, 596, 1278, 1598
MaeII	ACGT	3 cut(s)	810, 939, 2075
MaeIII	GTNAC	7 cut(s)	20, 390, 403, 649, 1549, 1570, 2060
MalI	GATC	3 cut(s)	276, 1395, 1737
MboI	GATC	3 cut(s)	274, 1393, 1735
MhlI	GDGCHC	4 cut(s)	193, 720, 732, 1996
MlsI	TGGCCA	2 cut(s)	1847, 2126
MluCI	AATT	6 cut(s)	931, 1272, 1342, 1437, 1864, 1901
MluNI	TGGCCA	2 cut(s)	1847, 2126
MlyI	GAGTC	3 cut(s)	116, 521, 853
MmeI	TCCRAC	1 cut(s)	2121
Mox20I	TGGCCA	2 cut(s)	1847, 2126
Mph1103I	ATGCAT	1 cut(s)	1719
MscI	TGGCCA	2 cut(s)	1847, 2126
MslI	CAYNNNNRTG	2 cut(s)	984, 1722
Msp20I	TGGCCA	2 cut(s)	1847, 2126
MspA1I	CMGCKG	2 cut(s)	1424, 1526
MspI	CCGG	5 cut(s)	265, 401, 1431, 1486, 1997
MspR9I	CCNGG	3 cut(s)	265, 856, 1432
Mva1269I	GAATGC	2 cut(s)	884, 1501
MvaI	CCWGG	1 cut(s)	856
MvnI	CGCG	2 cut(s)	1073, 1760
MwoI	GCNNNNNNNGC	4 cut(s)	548, 1298, 1844, 1981
NciI	CCSGG	2 cut(s)	265, 1432
NdeII	GATC	3 cut(s)	274, 1393, 1735
NlaIII	CATG	5 cut(s)	667, 1313, 1677, 1717, 2035
NlaIV	GGNNCC	3 cut(s)	821, 1420, 1995
NmeAIII	GCCGAG	1 cut(s)	1987
NmuCI	GTSAC	4 cut(s)	390, 649, 1549, 2060
NruI	TCGCGA	2 cut(s)	1073, 1760
NsbI	TGCGCA	1 cut(s)	482
NsiI	ATGCAT	1 cut(s)	1719
NspI	RCATGY	1 cut(s)	1677
PciSI	GCTCTTC	1 cut(s)	737
PctI	GAATGC	2 cut(s)	884, 1501
PfeI	GAWTC	7 cut(s)	775, 961, 968, 1691, 1780, 1956, 2098
PflFI	GACNNNGTC	1 cut(s)	1754
PflMI	CCANNNNNTGG	1 cut(s)	1645
PinAI	ACCGGT	2 cut(s)	400, 1996
PkrI	GCNGC	6 cut(s)	829, 928, 1300, 1303, 1423, 1590
PleI	GAGTC	3 cut(s)	115, 520, 853
PpsI	GAGTC	3 cut(s)	115, 520, 853
PpuMI	RGGWCCY	1 cut(s)	2146
PshAI	GACNNNNGTC	1 cut(s)	1925
PshBI	ATTAAT	2 cut(s)	564, 2184
PsiI	TTATAA	1 cut(s)	1661
Psp124BI	GAGCTC	1 cut(s)	193
Psp5II	RGGWCCY	1 cut(s)	2146
Psp6I	CCWGG	1 cut(s)	854
PspEI	GGTNACC	2 cut(s)	390, 1570
PspGI	CCWGG	1 cut(s)	854
PspN4I	GGNNCC	3 cut(s)	821, 1420, 1995
PspPI	GGNCC	3 cut(s)	819, 2050, 2146
PspPPI	RGGWCCY	1 cut(s)	2146
PstNI	CAGNNNCTG	1 cut(s)	110
PsyI	GACNNNGTC	1 cut(s)	1754
RruI	TCGCGA	2 cut(s)	1073, 1760
RsaI	GTAC	3 cut(s)	496, 1478, 1967
RsaNI	GTAC	3 cut(s)	495, 1477, 1966
RseI	CAYNNNNRTG	2 cut(s)	984, 1722
SacI	GAGCTC	1 cut(s)	193
SapI	GCTCTTC	1 cut(s)	737
SatI	GCNGC	6 cut(s)	828, 927, 1299, 1302, 1422, 1589
Sau3AI	GATC	3 cut(s)	274, 1393, 1735
Sau96I	GGNCC	3 cut(s)	819, 2050, 2146
ScaI	AGTACT	1 cut(s)	1967
SchI	GAGTC	3 cut(s)	116, 521, 853
ScrFI	CCNGG	3 cut(s)	265, 856, 1432
SduI	GDGCHC	4 cut(s)	193, 720, 732, 1996
SfaNI	GCATC	4 cut(s)	83, 468, 1726, 1752
SfcI	CTRYAG	2 cut(s)	288, 1077
SinI	GGWCC	2 cut(s)	819, 2146
SmiMI	CAYNNNNRTG	2 cut(s)	984, 1722
SmlI	CTYRAG	1 cut(s)	2175
SmoI	CTYRAG	1 cut(s)	2175
SpeI	ACTAGT	1 cut(s)	1277
Sse9I	AATT	6 cut(s)	931, 1272, 1342, 1437, 1864, 1901
SsiI	CCGC	6 cut(s)	825, 1144, 1187, 1422, 1526, 1982
SspMI	CTAG	5 cut(s)	186, 305, 596, 1278, 1598
SstI	GAGCTC	1 cut(s)	193
StyD4I	CCNGG	3 cut(s)	263, 854, 1430
StyI	CCWWGG	1 cut(s)	87
TaaI	ACNGT	8 cut(s)	819, 1078, 1096, 1576, 1754, 1795, 1897, 2060
TaiI	ACGT	3 cut(s)	813, 942, 2078
TaqI	TCGA	4 cut(s)	954, 1738, 1919, 2157
TasI	AATT	6 cut(s)	931, 1272, 1342, 1437, 1864, 1901
TatI	WGTACW	3 cut(s)	494, 1476, 1965
TauI	GCSGC	1 cut(s)	1424
TfiI	GAWTC	7 cut(s)	775, 961, 968, 1691, 1780, 1956, 2098
TscAI	CASTG	4 cut(s)	643, 1800, 1902, 2065
TseFI	GTSAC	4 cut(s)	390, 649, 1549, 2060
TseI	GCWGC	5 cut(s)	827, 926, 1298, 1301, 1588
Tsp45I	GTSAC	4 cut(s)	390, 649, 1549, 2060
TspDTI	ATGAA	6 cut(s)	652, 748, 1121, 1154, 1793, 2048
TspGWI	ACGGA	1 cut(s)	39
TspRI	CASTG	4 cut(s)	643, 1800, 1902, 2065
Tth111I	GACNNNGTC	1 cut(s)	1754
Van91I	CCANNNNNTGG	1 cut(s)	1645
VneI	GTGCAC	1 cut(s)	728
VpaK11BI	GGWCC	2 cut(s)	819, 2146
VspI	ATTAAT	2 cut(s)	564, 2184
XapI	RAATTY	2 cut(s)	931, 1901
XbaI	TCTAGA	2 cut(s)	304, 1597
XceI	RCATGY	1 cut(s)	1677
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1642
XmiI	GTMKAC	1 cut(s)	291
XspI	CTAG	5 cut(s)	186, 305, 596, 1278, 1598
ZrmI	AGTACT	1 cut(s)	1967
Zsp2I	ATGCAT	1 cut(s)	1719

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.