Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0000828

Physical Location & Seq

Genomic Coordinates Forward (+)

12855614 .. 12858736

Gene Structure 3123 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G006300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2091 bp

ATGCAAAGCGAAGAGGGAAATGAAGCAGCAGACGGTTCTTCTTCAGCTCCAAAGCAAAGCTCAATGGACTCTCCCAACAGATTGCCAGCTGAAATTTCCAATGAGACTCAAACTACCAGAAAGTCATTCAATCGGTCACTTATCTCCAAACCCAAGTCCAGAAAGTCGTTCAACAGGTCAGTTCTCTCCAAGCCCAAGTCCAGATTTGGGGAACCGTCAGTGCCCATTGACTCTGATTTGTTTGAAGAGAGCATTAGCGTGGAAAATTCACCTTCTAATAGTAATATGGGTTCATTCAATAGGGCCACTAATAATTCCCCTGATTTTAGAACTGTCTCCATCAGCTCTGCATGGAACCCAGTAATGGCGTCTCCAGGCAGGGCCAAAGCTGAAGAGGAGGACGAAAAGTTTTACAACGTGATCAGGGTATTGATTGAGTGGACTGTTTTCCTTTGCATTTTGGGTGTTTTAGTGGCTAGCTTGACTGTCGAGAAACTCGAGAAGCTTAAGGTTTGGGGGCTGGAGTTTTGGAAATGGTGTGTGCTTATGATGGTAGTATTCGGTGGCACGCTGCTCACATATTGGTTCACGCATTTCGTAGTCCTTTTCACCAAGAGGAACATACTGCTCAGGAAAAAGCTGTTGTACTATGTTTATGGAGTGAAGAAGAGTGTTCAGGTTTTCATGTGGTTGTCTTTGGTTCTTCTAACGTGGTTACTATTGTTCAATCCTAGTCGTCATGAGATTAAGCGATCAAAGACTCGTACCAACATTTTGAAATATGTTACTTGGACTCTTGCTTCGGCTGTTATTGGGGCATTTCTGTGGTTGTTGAAAACTCTGTTGTTTAAGATCGTAGCACTAAAGTTCCATCTGCGCCACACCTACTTTGATAGAGTTCAAGAGTCGATCTTCCATCAGTACATTCTTCAGACACTTTCAGGGCATCCAGTTATAGAGGAAGCCAAGAGGATCGGGAGATCACCGAGCACTGGTCAACTGAGTATCATAAGCACCAGGAAGGTCATTGATGTGGGAAAGCTTCAAAAGATGAAGCAGGTTTCAGCTTCTACCATGGAGGTGTTGGTGGATGCACTTTCAAGTTCAGCAGGGCTATCCACTTTCTCCTATACGTTGGATCGAGACCGAGGACTTAAACAGAAAGAAATGCAGATTGTCAATGAGATTCAAGCAATTGCTGCTGCCTCCCATATTTTTACCAATGTTGCTCAGCGTGATTACGAGTACATTGAGGAGGACGACCTCAGGAGGTTCATGATTAATGAAGACGTGGAACTTGTGTTCCGATTGATTGATGTCGGAGAGACGGGAAAAATTGACAAAAGAGCTCTAACAGATTGGGTGGTCAAGGTCTACAATGGTCGCAAAGCATTAGATCATGCTCTAACCGACACCAAAGCAGCTGTAAAGCAATTGAACGAAATTGTGACCGGCATCCTGATTGTTATTATCATTGTTGTGTGGCTTATATTAATGGAAGTTGCAACCACGAAAGTACTTGTTTTCATCTCATCACAGCTCGTATTGGCAGCCTTTATCTTTGGAAACACTTGCAAGAACATATTTGAAGCTATCGTATTTGTCTTTGTAATACATCCATTTGATGTTGGTGACCGCTGTCTTGTTGATGGAGTTCCGCTGAGGGTTGAGGAGATGAACATCCTTACTACAGTTTTCTTGAAACTAAATAATGAGAGGGTATATTATCCAAACTCAGTTTTATCAACAAAGACCATTAGCAATTATTATAGAAGTTCAGACATGGGAGATACCGTGGATTTCTCAATCGATTTAATTCCAGTTGACAGGATTAACCTGCTAAAAGCGAAAATAAAGAGTTATATGGAGAGTAACCCGCAGTATTGGCACCCAGATCACAATGTGGTGGTGATTGAGATTGAAAATGTGAATAAGTTAAAGATGGCACTCTATTTTAATCACACTATGAACTTTCAAGAATTTAGGGAAATAAATAAGCGGAGATCTGAGCTGGTGATTGAATTGATGAGAATATTTGAAGAGCTAAACATTGTATACAATCTGCTTCCTCACGATGTCAGCGTGGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

696

Amino Acids

79.72

Weight (kDa)

8.99

Isoelectric Point (pI)

44.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	525 - 590	1.1e-08	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000828)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12080 AT5G12080 AT5G12080 AT5G19520 AT5G19520
fragaria_vesca	FvH4_2g31211 FvH4_2g31220 FvH4_2g31230 FvH4_2g31240
malus_domestica	MD08G1070000.v1.1 MD15G1052100.v1.1
prunus_persica	Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1
pyrus_communis	pycom08g05800 pycom15g04990 pycom16g12570
rosa_chinensis	RchiOBHm_Chr1g0326931 RchiOBHm_Chr1g0326941 RchiOBHm_Chr6g0276141 RchiOBHm_Chr6g0283901 RchiOBHm_Chr6g0311561 RchiOBHm_Chr6g0311571 RchiOBHm_Chr6g0311581 RchiOBHm_Chr6g0311591
rosa_laevigata	RLG00000010389 RLG00000010390 RLG00000010391 RLG00000010393 RLG00000012788 RLG00000030050
rosa_multiflora	Rmu_sc0003315.1_g000003 Rmu_sc0006781.1_g000009 Rmu_sc0007556.1_g000006 Rmu_sc0018059.1_g000002 Rmu_sc0018059.1_g000003
rosa_roxburghii	Rroxscaffold_4G00323490 Rroxscaffold_7G00157470 Rroxscaffold_7G00157480 Rroxscaffold_7G00157490 Rroxscaffold_7G00185080
rosa_rugosa	Rorug01G0062100 Rorug01G0062300 Rorug06G0158200 Rorug06G0158300 Rorug06G0389800.1 Rorug06G0389900
rosa_samantha	Rh1AG079400 Rh1BG063600 Rh1CG077000 Rh1DG083500 Rh6AG504700 Rh6AG504800 Rh6AG504900 Rh6BG272400 Rh6BG515600 Rh6BG515700 Rh6BG515800 Rh6CG521400 Rh6CG521600 Rh6DG266500 Rh6DG507300 Rh6DG507400
rosa_wichuraiana	Rw1G006300 Rw6G018310 Rw6G023400 Rw6G043960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1048, 1845
AccB1I	GGYRCC	1 cut(s)	1887
AccI	GTMKAC	2 cut(s)	1374, 2055
AciI	CCGC	4 cut(s)	1636, 1658, 1877, 1999
AclWI	GGATC	2 cut(s)	980, 1146
AcsI	RAATTY	3 cut(s)	93, 265, 1979
AcuI	CTGAAG	3 cut(s)	27, 411, 914
AcyI	GRCGYC	1 cut(s)	368
AdeI	CACNNNGTG	1 cut(s)	1903
AfaI	GTAC	5 cut(s)	647, 766, 923, 1247, 1518
AfiI	CCNNNNNNNGG	3 cut(s)	207, 364, 992
AflII	CTTAAG	1 cut(s)	506
AjiI	CACGTC	1 cut(s)	1291
AjnI	CCWGG	2 cut(s)	373, 1016
AloI	GAACNNNNNNTCC	4 cut(s)	1286, 1287, 1318, 1319
Alw21I	GWGCWC	2 cut(s)	992, 1351
Alw26I	GTCTC	5 cut(s)	98, 340, 375, 1137, 1319
AlwI	GGATC	2 cut(s)	980, 1146
Ama87I	CYCGRG	1 cut(s)	497
AoxI	GGCC	3 cut(s)	303, 381, 2085
ApeKI	GCWGC	6 cut(s)	26, 571, 1199, 1202, 1421, 1550
ApoI	RAATTY	3 cut(s)	93, 265, 1979
ArsI	GACNNNNNNTTYG	2 cut(s)	784, 816
AseI	ATTAAT	2 cut(s)	1281, 1493
AspLEI	GCGC	1 cut(s)	879
AspS9I	GGNCC	2 cut(s)	303, 381
AsuHPI	GGTGA	6 cut(s)	261, 601, 975, 1643, 1921, 2026
AsuNHI	GCTAGC	1 cut(s)	476
AvaI	CYCGRG	1 cut(s)	497
AxyI	CCTNAGG	1 cut(s)	1265
BaeGI	GKGCMC	1 cut(s)	225
BanI	GGYRCC	1 cut(s)	1887
BanII	GRGCYC	1 cut(s)	1351
BbsI	GAAGAC	1 cut(s)	1293
Bbv12I	GWGCWC	2 cut(s)	992, 1351
BbvCI	CCTCAGC	1 cut(s)	1661
BbvI	GCAGC	6 cut(s)	38, 558, 1186, 1189, 1433, 1562
BccI	CCATC	6 cut(s)	347, 544, 879, 924, 1643, 1936
BciT130I	CCWGG	2 cut(s)	375, 1018
BclI	TGATCA	1 cut(s)	420
BcoDI	GTCTC	5 cut(s)	98, 340, 375, 1137, 1319
BfaI	CTAG	2 cut(s)	477, 732
BfmI	CTRYAG	1 cut(s)	1689
BfrI	CTTAAG	1 cut(s)	506
BfuAI	ACCTGC	2 cut(s)	1048, 1845
BglII	AGATCT	1 cut(s)	2003
BisI	GCNGC	6 cut(s)	27, 572, 1200, 1203, 1422, 1551
BlpI	GCTNAGC	1 cut(s)	1230
BlsI	GCNGC	6 cut(s)	28, 573, 1201, 1204, 1423, 1552
BmcAI	AGTACT	1 cut(s)	1518
Bme1390I	CCNGG	2 cut(s)	375, 1018
BmeT110I	CYCGRG	1 cut(s)	497
BmgBI	CACGTC	1 cut(s)	1291
BmgT120I	GGNCC	2 cut(s)	303, 381
BmiI	GGNNCC	3 cut(s)	213, 356, 1889
BmrFI	CCNGG	2 cut(s)	375, 1018
BmrI	ACTGGG	1 cut(s)	353
BmsI	GCATC	3 cut(s)	955, 1081, 1464
BmtI	GCTAGC	1 cut(s)	480
BmuI	ACTGGG	1 cut(s)	353
BoxI	GACNNNNGTC	1 cut(s)	1638
BpiI	GAAGAC	1 cut(s)	1293
BpmI	CTGGAG	2 cut(s)	357, 542
Bpu10I	CCTNAGC	2 cut(s)	629, 1661
Bpu1102I	GCTNAGC	1 cut(s)	1230
Bsa29I	ATCGAT	1 cut(s)	1809
BsaBI	GATNNNNATC	1 cut(s)	1679
BsaHI	GRCGYC	1 cut(s)	368
BsaI	GGTCTC	1 cut(s)	1137
BsaJI	CCNNGG	3 cut(s)	1074, 1147, 1794
BsaXI	ACNNNNNCTCC	2 cut(s)	1779, 1809
Bsc4I	CCNNNNNNNGG	3 cut(s)	207, 364, 992
Bse118I	RCCGGY	1 cut(s)	1451
Bse1I	ACTGG	4 cut(s)	359, 950, 997, 1820
Bse21I	CCTNAGG	1 cut(s)	1265
Bse8I	GATNNNNATC	1 cut(s)	1679
BseBI	CCWGG	2 cut(s)	375, 1018
BseCI	ATCGAT	1 cut(s)	1809
BseDI	CCNNGG	3 cut(s)	1074, 1147, 1794
BseGI	GGATG	5 cut(s)	946, 1096, 1455, 1615, 1680
BseJI	GATNNNNATC	1 cut(s)	1679
BseLI	CCNNNNNNNGG	3 cut(s)	207, 364, 992
BseMII	CTCAG	7 cut(s)	643, 992, 1244, 1279, 1652, 1749, 1998
BseNI	ACTGG	4 cut(s)	359, 950, 997, 1820
BseRI	GAGGAG	3 cut(s)	410, 1268, 1685
BseSI	GKGCMC	1 cut(s)	225
BseXI	GCAGC	6 cut(s)	38, 558, 1186, 1189, 1433, 1562
BshFI	GGCC	3 cut(s)	305, 383, 2087
BshNI	GGYRCC	1 cut(s)	1887
BshVI	ATCGAT	1 cut(s)	1809
BsiHKAI	GWGCWC	2 cut(s)	992, 1351
BsiHKCI	CYCGRG	1 cut(s)	497
BsiSI	CCGG	1 cut(s)	1452
BslI	CCNNNNNNNGG	3 cut(s)	207, 364, 992
BsmAI	GTCTC	5 cut(s)	98, 340, 375, 1137, 1319
BsmBI	CGTCTC	2 cut(s)	375, 1319
BsnI	GGCC	3 cut(s)	305, 383, 2087
Bso31I	GGTCTC	1 cut(s)	1137
BsoBI	CYCGRG	1 cut(s)	497
Bsp1286I	GDGCHC	3 cut(s)	225, 992, 1351
Bsp1720I	GCTNAGC	1 cut(s)	1230
Bsp19I	CCATGG	1 cut(s)	1074
BspACI	CCGC	4 cut(s)	1636, 1658, 1877, 1999
BspANI	GGCC	3 cut(s)	305, 383, 2087
BspCNI	CTCAG	7 cut(s)	642, 993, 1243, 1278, 1653, 1748, 1999
BspDI	ATCGAT	1 cut(s)	1809
BspHI	TCATGA	2 cut(s)	739, 1275
BspLI	GGNNCC	3 cut(s)	213, 356, 1889
BspMI	ACCTGC	2 cut(s)	1048, 1845
BspOI	GCTAGC	1 cut(s)	480
BspPI	GGATC	2 cut(s)	980, 1146
BspQI	GCTCTTC	1 cut(s)	2034
BspT107I	GGYRCC	1 cut(s)	1887
BspTI	CTTAAG	1 cut(s)	506
BspTNI	GGTCTC	1 cut(s)	1137
BsrFI	RCCGGY	1 cut(s)	1451
BsrI	ACTGG	4 cut(s)	359, 950, 997, 1820
BssAI	RCCGGY	1 cut(s)	1451
BssECI	CCNNGG	3 cut(s)	1074, 1147, 1794
BssNAI	GTATAC	1 cut(s)	2056
BssNI	GRCGYC	1 cut(s)	368
BssT1I	CCWWGG	1 cut(s)	1074
Bst1107I	GTATAC	1 cut(s)	2056
Bst2UI	CCWGG	2 cut(s)	375, 1018
Bst4CI	ACNGT	7 cut(s)	35, 216, 334, 445, 487, 1693, 1795
Bst6I	CTCTTC	5 cut(s)	6, 240, 387, 662, 2034
BstACI	GRCGYC	1 cut(s)	368
BstAFI	CTTAAG	1 cut(s)	506
BstAPI	GCANNNNNTGC	1 cut(s)	1199
BstC8I	GCNNGC	3 cut(s)	87, 478, 569
BstDEI	CTNAG	7 cut(s)	629, 1001, 1230, 1265, 1661, 1735, 2007
BstDSI	CCRYGG	2 cut(s)	1074, 1794
BstEII	GGTNACC	1 cut(s)	1631
BstF5I	GGATG	5 cut(s)	946, 1096, 1455, 1615, 1680
BstHHI	GCGC	1 cut(s)	879
BstMAI	GTCTC	5 cut(s)	98, 340, 375, 1137, 1319
BstMWI	GCNNNNNNNGC	2 cut(s)	1199, 1885
BstNI	CCWGG	2 cut(s)	375, 1018
BstPAI	GACNNNNGTC	1 cut(s)	1638
BstPI	GGTNACC	1 cut(s)	1631
BstSCI	CCNGG	2 cut(s)	373, 1016
BstSFI	CTRYAG	1 cut(s)	1689
BstSLI	GKGCMC	1 cut(s)	225
BstV1I	GCAGC	6 cut(s)	38, 558, 1186, 1189, 1433, 1562
BstV2I	GAAGAC	1 cut(s)	1293
BstX2I	RGATCY	1 cut(s)	2003
BstYI	RGATCY	1 cut(s)	2003
BstZ17I	GTATAC	1 cut(s)	2056
Bsu15I	ATCGAT	1 cut(s)	1809
Bsu36I	CCTNAGG	1 cut(s)	1265
BsuRI	GGCC	3 cut(s)	305, 383, 2087
BsuTUI	ATCGAT	1 cut(s)	1809
BtgI	CCRYGG	2 cut(s)	1074, 1794
BtrI	CACGTC	1 cut(s)	1291
BtsCI	GGATG	5 cut(s)	946, 1096, 1455, 1615, 1680
BtsIMutI	CAGTG	2 cut(s)	225, 990
BveI	ACCTGC	2 cut(s)	1048, 1845
Cac8I	GCNNGC	3 cut(s)	87, 478, 569
CciI	TCATGA	2 cut(s)	739, 1275
CfoI	GCGC	1 cut(s)	879
Cfr10I	RCCGGY	1 cut(s)	1451
Cfr13I	GGNCC	2 cut(s)	303, 381
ClaI	ATCGAT	1 cut(s)	1809
CseI	GACGC	1 cut(s)	357
Csp6I	GTAC	5 cut(s)	646, 765, 922, 1246, 1517
CviAII	CATG	7 cut(s)	351, 685, 740, 1075, 1276, 1400, 1783
CviQI	GTAC	5 cut(s)	646, 765, 922, 1246, 1517
DdeI	CTNAG	7 cut(s)	629, 1001, 1230, 1265, 1661, 1735, 2007
DraIII	CACNNNGTG	1 cut(s)	1903
Eam1104I	CTCTTC	5 cut(s)	6, 240, 387, 662, 2034
EarI	CTCTTC	5 cut(s)	6, 240, 387, 662, 2034
Ecl136II	GAGCTC	1 cut(s)	1349
Eco130I	CCWWGG	1 cut(s)	1074
Eco24I	GRGCYC	1 cut(s)	1351
Eco31I	GGTCTC	1 cut(s)	1137
Eco53kI	GAGCTC	1 cut(s)	1349
Eco57I	CTGAAG	3 cut(s)	27, 411, 914
Eco81I	CCTNAGG	1 cut(s)	1265
Eco88I	CYCGRG	1 cut(s)	497
Eco91I	GGTNACC	1 cut(s)	1631
EcoICRI	GAGCTC	1 cut(s)	1349
EcoO65I	GGTNACC	1 cut(s)	1631
EcoRII	CCWGG	2 cut(s)	373, 1016
EcoT14I	CCWWGG	1 cut(s)	1074
EcoT38I	GRGCYC	1 cut(s)	1351
ErhI	CCWWGG	1 cut(s)	1074
Esp3I	CGTCTC	2 cut(s)	375, 1319
FaeI	CATG	7 cut(s)	354, 688, 743, 1078, 1279, 1403, 1786
FatI	CATG	7 cut(s)	350, 684, 739, 1074, 1275, 1399, 1782
FauI	CCCGC	1 cut(s)	1884
FbaI	TGATCA	1 cut(s)	420
FblI	GTMKAC	2 cut(s)	1374, 2055
Fnu4HI	GCNGC	6 cut(s)	27, 572, 1200, 1203, 1422, 1551
FokI	GGATG	5 cut(s)	933, 1103, 1442, 1602, 1667
FriOI	GRGCYC	1 cut(s)	1351
Fsp4HI	GCNGC	6 cut(s)	27, 572, 1200, 1203, 1422, 1551
FspBI	CTAG	2 cut(s)	477, 732
GlaI	GCGC	1 cut(s)	878
GluI	GCNGC	6 cut(s)	27, 572, 1200, 1203, 1422, 1551
GsuI	CTGGAG	2 cut(s)	357, 542
HaeIII	GGCC	3 cut(s)	305, 383, 2087
HapII	CCGG	1 cut(s)	1452
HgaI	GACGC	1 cut(s)	357
HhaI	GCGC	1 cut(s)	879
Hin1I	GRCGYC	1 cut(s)	368
Hin1II	CATG	7 cut(s)	354, 688, 743, 1078, 1279, 1403, 1786
Hin6I	GCGC	1 cut(s)	877
HinP1I	GCGC	1 cut(s)	877
HincII	GTYRAC	2 cut(s)	998, 1825
HindII	GTYRAC	2 cut(s)	998, 1825
HindIII	AAGCTT	2 cut(s)	503, 1040
HinfI	GANTC	7 cut(s)	68, 106, 230, 760, 793, 905, 1186
HpaII	CCGG	1 cut(s)	1452
HphI	GGTGA	6 cut(s)	261, 601, 975, 1643, 1921, 2026
Hpy166II	GTNNAC	6 cut(s)	441, 588, 998, 1375, 1825, 2056
Hpy188I	TCNGA	6 cut(s)	235, 933, 1307, 1322, 1780, 2008
Hpy8I	GTNNAC	6 cut(s)	441, 588, 998, 1375, 1825, 2056
HpyAV	CCTTC	2 cut(s)	282, 1015
HpyCH4III	ACNGT	7 cut(s)	35, 216, 334, 445, 487, 1693, 1795
HpyCH4IV	ACGT	4 cut(s)	417, 710, 1133, 1290
HpyCH4V	TGCA	7 cut(s)	4, 350, 456, 1094, 1171, 1505, 1575
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1199, 1885
HpyF3I	CTNAG	7 cut(s)	629, 1001, 1230, 1265, 1661, 1735, 2007
HpySE526I	ACGT	4 cut(s)	417, 710, 1133, 1290
Hsp92I	GRCGYC	1 cut(s)	368
Hsp92II	CATG	7 cut(s)	354, 688, 743, 1078, 1279, 1403, 1786
HspAI	GCGC	1 cut(s)	877
Ksp22I	TGATCA	1 cut(s)	420
LguI	GCTCTTC	1 cut(s)	2034
LmnI	GCTCC	1 cut(s)	52
Lsp1109I	GCAGC	6 cut(s)	38, 558, 1186, 1189, 1433, 1562
LweI	GCATC	3 cut(s)	955, 1081, 1464
MaeI	CTAG	2 cut(s)	477, 732
MaeII	ACGT	4 cut(s)	417, 710, 1133, 1290
MaeIII	GTNAC	6 cut(s)	135, 714, 784, 1447, 1631, 1871
MfeI	CAATTG	2 cut(s)	1194, 1433
MflI	RGATCY	1 cut(s)	2003
MhlI	GDGCHC	3 cut(s)	225, 992, 1351
MlyI	GAGTC	6 cut(s)	62, 100, 224, 754, 787, 914
MmeI	TCCRAC	2 cut(s)	1116, 1300
MslI	CAYNNNNRTG	5 cut(s)	257, 823, 1031, 1079, 1478
MspA1I	CMGCKG	4 cut(s)	89, 1424, 1638, 1660
MspCI	CTTAAG	1 cut(s)	506
MspI	CCGG	1 cut(s)	1452
MspR9I	CCNGG	2 cut(s)	375, 1018
MunI	CAATTG	2 cut(s)	1194, 1433
MvaI	CCWGG	2 cut(s)	375, 1018
MwoI	GCNNNNNNNGC	2 cut(s)	1199, 1885
NcoI	CCATGG	1 cut(s)	1074
NheI	GCTAGC	1 cut(s)	476
NlaIII	CATG	7 cut(s)	354, 688, 743, 1078, 1279, 1403, 1786
NlaIV	GGNNCC	3 cut(s)	213, 356, 1889
NmuCI	GTSAC	3 cut(s)	135, 1447, 1631
PaeR7I	CTCGAG	1 cut(s)	497
PagI	TCATGA	2 cut(s)	739, 1275
PciSI	GCTCTTC	1 cut(s)	2034
PcsI	WCGNNNNNNNCGW	2 cut(s)	495, 2079
PfeI	GAWTC	1 cut(s)	1186
PkrI	GCNGC	6 cut(s)	28, 573, 1201, 1204, 1423, 1552
PleI	GAGTC	6 cut(s)	62, 100, 224, 754, 787, 913
PpsI	GAGTC	6 cut(s)	62, 100, 224, 754, 787, 913
PshAI	GACNNNNGTC	1 cut(s)	1638
PshBI	ATTAAT	2 cut(s)	1281, 1493
Psp124BI	GAGCTC	1 cut(s)	1351
Psp6I	CCWGG	2 cut(s)	373, 1016
PspEI	GGTNACC	1 cut(s)	1631
PspGI	CCWGG	2 cut(s)	373, 1016
PspN4I	GGNNCC	3 cut(s)	213, 356, 1889
PspPI	GGNCC	2 cut(s)	303, 381
PsrI	GAACNNNNNNTAC	2 cut(s)	274, 306
PsuI	RGATCY	1 cut(s)	2003
PvuII	CAGCTG	2 cut(s)	89, 1424
RsaI	GTAC	5 cut(s)	647, 766, 923, 1247, 1518
RsaNI	GTAC	5 cut(s)	646, 765, 922, 1246, 1517
RseI	CAYNNNNRTG	5 cut(s)	257, 823, 1031, 1079, 1478
SacI	GAGCTC	1 cut(s)	1351
SapI	GCTCTTC	1 cut(s)	2034
SatI	GCNGC	6 cut(s)	27, 572, 1200, 1203, 1422, 1551
Sau96I	GGNCC	2 cut(s)	303, 381
ScaI	AGTACT	1 cut(s)	1518
SchI	GAGTC	6 cut(s)	62, 100, 224, 754, 787, 914
ScrFI	CCNGG	2 cut(s)	375, 1018
SduI	GDGCHC	3 cut(s)	225, 992, 1351
SfaNI	GCATC	3 cut(s)	955, 1081, 1464
SfcI	CTRYAG	1 cut(s)	1689
Sfr274I	CTCGAG	1 cut(s)	497
SlaI	CTCGAG	1 cut(s)	497
SmiMI	CAYNNNNRTG	5 cut(s)	257, 823, 1031, 1079, 1478
SmlI	CTYRAG	2 cut(s)	497, 506
SmoI	CTYRAG	2 cut(s)	497, 506
SsiI	CCGC	4 cut(s)	1636, 1658, 1877, 1999
SspI	AATATT	1 cut(s)	2034
SspMI	CTAG	2 cut(s)	477, 732
SstI	GAGCTC	1 cut(s)	1351
StyD4I	CCNGG	2 cut(s)	373, 1016
StyI	CCWWGG	1 cut(s)	1074
TaaI	ACNGT	7 cut(s)	35, 216, 334, 445, 487, 1693, 1795
TaiI	ACGT	4 cut(s)	420, 713, 1136, 1293
TaqI	TCGA	5 cut(s)	489, 498, 908, 1141, 1809
TaqII	GACCGA	2 cut(s)	123, 1161
TatI	WGTACW	4 cut(s)	645, 921, 1245, 1516
TfiI	GAWTC	1 cut(s)	1186
TscAI	CASTG	2 cut(s)	225, 997
TseFI	GTSAC	3 cut(s)	135, 1447, 1631
TseI	GCWGC	6 cut(s)	26, 571, 1199, 1202, 1421, 1550
Tsp45I	GTSAC	3 cut(s)	135, 1447, 1631
TspDTI	ATGAA	9 cut(s)	36, 282, 673, 1067, 1264, 1299, 1516, 1691, 1982
TspRI	CASTG	2 cut(s)	225, 997
Vha464I	CTTAAG	1 cut(s)	506
VspI	ATTAAT	2 cut(s)	1281, 1493
XapI	RAATTY	3 cut(s)	93, 265, 1979
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1081
XhoI	CTCGAG	1 cut(s)	497
XmiI	GTMKAC	2 cut(s)	1374, 2055
XspI	CTAG	2 cut(s)	477, 732
ZrmI	AGTACT	1 cut(s)	1518

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.