Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0000828

Physical Location & Seq

Genomic Coordinates Reverse (-)

22362163 .. 22370204

Gene Structure 8042 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000012788

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2145 bp

ATGCCGGAAAAGATGGGTGGTAACAGCTTCACCGAAGCCATCAATCTTGATTCTAGTGTTGTTTCGGCGCTGCCGGACATAGCCTCCAACATCTCAACGCTAGGTACCAGATTTGGTTCTGGGTGTCCAAATCCATATGGTTGGCTGTGTATTTGGGTTGTGTTTGGACCTAAGTTCAACCTGGTTCTAGGGATTTCGACGGCAAGGGTTCTCTCGCTGTGGTGGTTTTCTGATCGATCGAAGATGATGGTGGCGCCGCCGTCATGGTGGTGTCCAACAGGGCAGCTAGATTTGGGCCTCAGTAGGCTTCTTGGGCCTGTAGCTGGTTTTGGAGCCTTGTTAGTGTTGGGGTCCAAAATGTCTAGTGGGTCCTGGATGGCCTTCTTGGACCGCTGCGGGCTTGAAGCTTTGAAGATAAACTTTGGGCCTAGCTTTCTTGGGTCCAGAATGAGATTAGCTTACTCTAAACCCAAGTCGCGATTTGGTGAACTACCATATCACGCTGATTTGGATATGTTACTTGAAGACACTAGTAGTTTAGATGAGCCGGTTGGTGCAAGTTTTCATAGTAATAGCGGCTCATTCACTAGGACTGTCTCCAGCAGTTCTTCCAAGTCCCCAGCGATGCTGTCTCCGGAGAGGCCGAAGTATAAGGATAAAGAGCTCTACAAGAAGGCTAAATTGAATAGAGATAAGCAGTGGAAAGCATTTAACAAGAAGATCTTGATTAAGCCGACTGTTTTTCTGTGCATTTTGGTTTGTTTAGTGGCTAGCTTCACTTATGAGAAACTAAAGAATCTTATGGTTTGGGGATTGAGGATTTGGAAATGGTGTGTGCTTGTAATGGTGATGTACTGTGGCATGTTTATTGCGAATTGGTTTATGCGGTTTTTAATTTTTATGATTGAGACGAAGTTCTTGCTTAGGAAACAGAAGGTGTTGTATTGTATTCAAGGAATGGAGGAGAGTGCTCGGGTTTTCATATGGTTGGGTTTGGTTCTTCTCACATGGCTACTCCTGTTCAACCATGGGGTTGAGATTGAGCGATCAAAGACTTGCACCAGGATTTTGCATTATGTTTTGTGGACTCTTGTTTCTGTTCTCGTTGGGGCATTTTTGTGGCTGTTAAAAACTCTTCTGCTTAAGTTCTTAGCATTTGATTTCCAATCATACACCTTCTTTGATAGAATCCAAGAATCAATCTTCCATCAACAAAGCAGGGTCACGGACAGAAACAATGCTGAGAGCGAGAAACAGGCCATTGATATGGGAGACCTTCAAAAGACGAAACAAAAGAAGGTTTCACCTTGGACCATGAAGGTATTGGTAGATGCAGTTTCTACTTCAGGGCTATCCACCATGTCCCATACATTCAATGATAGGAAAGATGCAGTGGATGAAGGGAAATATAAGAAGATTACCAGTGAGATAGAAGCAGCTACTGCTGCCTATCATATTTTTAGGAACTGCTCACTTATGATAAATATCCCGCTTCCCTTCTCATATTTAGTTTTGTTTGGTATGAGTAGGTACATTTCCGAATCGGACCTTATGAAGTTCATGACTAAGGAAGAGGTGAAGCTTGTGTGGCCGCTGATTGACGTAGCAAAGACTCGACAAATTGGCAAAAATGATTCAACAGATTACGTGGTAAAGGCCGACAAAGATCGCGAAGCATTAGCTTATGCTTTACAAGACACCAAAACAGCTGAAAAGCAATTTGACAGACTAGTGACTGGCATCCTGATTGTTATTACCGTTATAGTGTGGCTTCTATTGTTGGAAATTGCGAACACCAAAGTACTTGTTTTACTCTCATCACAGATCGTATTAGCAGCTTTTATGTTTGGAAACAGATGCAAGAATATATTTGAAGCTATCGTCTTTGTATTTGTAATACATCCGTTTGATGTTGGTGACCGCTGTGTTGTTGATGACGTTCCGTTGATAGTTGAGGAGATTAACAACTTTACTACATTCTTGAAGTCTAACAATGAGAAGGTATACTATCCGAATTCAGTTTTATCCACAAAAACCATCAGCAATTATTACAGAAGTTCAGATATGAAAGATACTGTGGAGTTCTCGATTGCTTTGACACCTGTGGAGAAGATAGTCAAGCTCAAAGAGAGACTTAAGGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

715

Amino Acids

81.28

Weight (kDa)

9.18

Isoelectric Point (pI)

38.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	621 - 684	1.3e-06	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000828)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12080 AT5G12080 AT5G12080 AT5G19520 AT5G19520
fragaria_vesca	FvH4_2g31211 FvH4_2g31220 FvH4_2g31230 FvH4_2g31240
malus_domestica	MD08G1070000.v1.1 MD15G1052100.v1.1
prunus_persica	Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1
pyrus_communis	pycom08g05800 pycom15g04990 pycom16g12570
rosa_chinensis	RchiOBHm_Chr1g0326931 RchiOBHm_Chr1g0326941 RchiOBHm_Chr6g0276141 RchiOBHm_Chr6g0283901 RchiOBHm_Chr6g0311561 RchiOBHm_Chr6g0311571 RchiOBHm_Chr6g0311581 RchiOBHm_Chr6g0311591
rosa_laevigata	RLG00000010389 RLG00000010390 RLG00000010391 RLG00000010393 RLG00000012788 RLG00000030050
rosa_multiflora	Rmu_sc0003315.1_g000003 Rmu_sc0006781.1_g000009 Rmu_sc0007556.1_g000006 Rmu_sc0018059.1_g000002 Rmu_sc0018059.1_g000003
rosa_roxburghii	Rroxscaffold_4G00323490 Rroxscaffold_7G00157470 Rroxscaffold_7G00157480 Rroxscaffold_7G00157490 Rroxscaffold_7G00185080
rosa_rugosa	Rorug01G0062100 Rorug01G0062300 Rorug06G0158200 Rorug06G0158300 Rorug06G0389800.1 Rorug06G0389900
rosa_samantha	Rh1AG079400 Rh1BG063600 Rh1CG077000 Rh1DG083500 Rh6AG504700 Rh6AG504800 Rh6AG504900 Rh6BG272400 Rh6BG515600 Rh6BG515700 Rh6BG515800 Rh6CG521400 Rh6CG521600 Rh6DG266500 Rh6DG507300 Rh6DG507400
rosa_wichuraiana	Rw1G006300 Rw6G018310 Rw6G023400 Rw6G043960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	104
AccB1I	GGYRCC	2 cut(s)	104, 253
AccI	GTMKAC	1 cut(s)	2004
AccII	CGCG	2 cut(s)	478, 1671
AccIII	TCCGGA	1 cut(s)	634
AciI	CCGC	8 cut(s)	257, 391, 396, 576, 886, 1490, 1592, 1921
AcoI	YGGCCR	1 cut(s)	1589
AcsI	RAATTY	1 cut(s)	2014
AcuI	CTGAAG	1 cut(s)	1329
AcyI	GRCGYC	1 cut(s)	254
AfaI	GTAC	4 cut(s)	106, 854, 1532, 1803
AfiI	CCNNNNNNNGG	1 cut(s)	323
AflII	CTTAAG	2 cut(s)	1142, 2135
AhlI	ACTAGT	2 cut(s)	530, 1729
AjnI	CCWGG	3 cut(s)	180, 371, 1061
Alw21I	GWGCWC	2 cut(s)	666, 973
Alw26I	GTCTC	5 cut(s)	601, 636, 902, 1266, 2125
AlwNI	CAGNNNCTG	1 cut(s)	1442
Ama87I	CYCGRG	1 cut(s)	972
Aor13HI	TCCGGA	1 cut(s)	634
AoxI	GGCC	8 cut(s)	295, 314, 378, 425, 641, 1257, 1589, 1656
ApeKI	GCWGC	6 cut(s)	70, 283, 393, 1436, 1445, 1835
ApoI	RAATTY	1 cut(s)	2014
Asp718I	GGTACC	1 cut(s)	104
AspLEI	GCGC	2 cut(s)	70, 256
AsuHPI	GGTGA	6 cut(s)	22, 497, 859, 1296, 1588, 1928
AsuNHI	GCTAGC	1 cut(s)	770
AvaI	CYCGRG	1 cut(s)	972
AvaII	GGWCC	7 cut(s)	167, 351, 369, 388, 441, 1311, 1546
BanI	GGYRCC	2 cut(s)	104, 253
BanII	GRGCYC	1 cut(s)	666
BbsI	GAAGAC	1 cut(s)	531
Bbv12I	GWGCWC	2 cut(s)	666, 973
BbvI	GCAGC	6 cut(s)	57, 295, 380, 1432, 1448, 1847
BccI	CCATC	6 cut(s)	7, 47, 241, 370, 1215, 2045
BceAI	ACGGC	2 cut(s)	216, 244
BcgI	CGANNNNNNTGC	2 cut(s)	901, 935
BciT130I	CCWGG	3 cut(s)	182, 373, 1063
BcoDI	GTCTC	5 cut(s)	601, 636, 902, 1266, 2125
BcuI	ACTAGT	2 cut(s)	530, 1729
BfmI	CTRYAG	1 cut(s)	318
BfoI	RGCGCY	2 cut(s)	71, 257
BfrI	CTTAAG	2 cut(s)	1142, 2135
BglII	AGATCT	1 cut(s)	720
BisI	GCNGC	9 cut(s)	71, 257, 284, 394, 577, 1437, 1446, 1592, 1836
BlsI	GCNGC	9 cut(s)	72, 258, 285, 395, 578, 1438, 1447, 1593, 1837
BmcAI	AGTACT	1 cut(s)	1803
Bme1390I	CCNGG	3 cut(s)	182, 373, 1063
Bme18I	GGWCC	7 cut(s)	167, 351, 369, 388, 441, 1311, 1546
BmeT110I	CYCGRG	1 cut(s)	972
BmiI	GGNNCC	6 cut(s)	106, 255, 334, 352, 370, 442
BmrFI	CCNGG	3 cut(s)	182, 373, 1063
BmsI	GCATC	5 cut(s)	615, 1321, 1378, 1749, 1847
BmtI	GCTAGC	1 cut(s)	774
BpiI	GAAGAC	1 cut(s)	531
BpmI	CTGGAG	1 cut(s)	583
Bpu10I	CCTNAGC	1 cut(s)	923
Bsa29I	ATCGAT	1 cut(s)	235
BsaAI	YACGTR	1 cut(s)	1648
BsaBI	GATNNNNATC	1 cut(s)	1484
BsaHI	GRCGYC	1 cut(s)	254
BsaI	GGTCTC	1 cut(s)	1266
BsaJI	CCNNGG	2 cut(s)	1027, 1307
BsaWI	WCCGGW	1 cut(s)	634
BsaXI	ACNNNNNCTCC	2 cut(s)	68, 98
Bsc4I	CCNNNNNNNGG	1 cut(s)	323
Bse118I	RCCGGY	1 cut(s)	547
Bse1I	ACTGG	2 cut(s)	1422, 1741
Bse8I	GATNNNNATC	1 cut(s)	1484
BseAI	TCCGGA	1 cut(s)	634
BseBI	CCWGG	3 cut(s)	182, 373, 1063
BseCI	ATCGAT	1 cut(s)	235
BseDI	CCNNGG	2 cut(s)	1027, 1307
BseGI	GGATG	4 cut(s)	381, 1402, 1740, 1900
BseJI	GATNNNNATC	1 cut(s)	1484
BseLI	CCNNNNNNNGG	1 cut(s)	323
BseMII	CTCAG	2 cut(s)	313, 1233
BseNI	ACTGG	2 cut(s)	1422, 1741
BseRI	GAGGAG	2 cut(s)	977, 1970
BseXI	GCAGC	6 cut(s)	57, 295, 380, 1432, 1448, 1847
BseYI	CCCAGC	1 cut(s)	619
Bsh1236I	CGCG	2 cut(s)	478, 1671
Bsh1285I	CGRYCG	1 cut(s)	239
BshFI	GGCC	8 cut(s)	297, 316, 380, 427, 643, 1259, 1591, 1658
BshNI	GGYRCC	2 cut(s)	104, 253
BshVI	ATCGAT	1 cut(s)	235
BsiEI	CGRYCG	1 cut(s)	239
BsiHKAI	GWGCWC	2 cut(s)	666, 973
BsiHKCI	CYCGRG	1 cut(s)	972
BsiSI	CCGG	4 cut(s)	5, 74, 548, 635
BslFI	GGGAC	2 cut(s)	601, 1348
BslI	CCNNNNNNNGG	1 cut(s)	323
BsmAI	GTCTC	5 cut(s)	601, 636, 902, 1266, 2125
BsmBI	CGTCTC	1 cut(s)	902
BsmFI	GGGAC	2 cut(s)	601, 1348
BsnI	GGCC	8 cut(s)	297, 316, 380, 427, 643, 1259, 1591, 1658
Bso31I	GGTCTC	1 cut(s)	1266
BsoBI	CYCGRG	1 cut(s)	972
Bsp1286I	GDGCHC	2 cut(s)	666, 973
Bsp13I	TCCGGA	1 cut(s)	634
Bsp143I	GATC	6 cut(s)	232, 236, 720, 1046, 1666, 1824
Bsp19I	CCATGG	1 cut(s)	1027
Bsp68I	TCGCGA	2 cut(s)	478, 1671
BspACI	CCGC	8 cut(s)	257, 391, 396, 576, 886, 1490, 1592, 1921
BspANI	GGCC	8 cut(s)	297, 316, 380, 427, 643, 1259, 1591, 1658
BspCNI	CTCAG	2 cut(s)	312, 1234
BspDI	ATCGAT	1 cut(s)	235
BspEI	TCCGGA	1 cut(s)	634
BspFNI	CGCG	2 cut(s)	478, 1671
BspHI	TCATGA	1 cut(s)	1560
BspLI	GGNNCC	6 cut(s)	106, 255, 334, 352, 370, 442
BspOI	GCTAGC	1 cut(s)	774
BspT107I	GGYRCC	2 cut(s)	104, 253
BspTI	CTTAAG	2 cut(s)	1142, 2135
BspTNI	GGTCTC	1 cut(s)	1266
BsrFI	RCCGGY	1 cut(s)	547
BsrI	ACTGG	2 cut(s)	1422, 1741
BssAI	RCCGGY	1 cut(s)	547
BssECI	CCNNGG	2 cut(s)	1027, 1307
BssMI	GATC	6 cut(s)	232, 236, 720, 1046, 1666, 1824
BssNAI	GTATAC	1 cut(s)	2005
BssNI	GRCGYC	1 cut(s)	254
BssT1I	CCWWGG	2 cut(s)	1027, 1307
Bst1107I	GTATAC	1 cut(s)	2005
Bst2UI	CCWGG	3 cut(s)	182, 373, 1063
Bst4CI	ACNGT	5 cut(s)	595, 739, 857, 1759, 2077
Bst6I	CTCTTC	2 cut(s)	1140, 1566
BstACI	GRCGYC	1 cut(s)	254
BstAFI	CTTAAG	2 cut(s)	1142, 2135
BstAPI	GCANNNNNTGC	1 cut(s)	1442
BstBAI	YACGTR	1 cut(s)	1648
BstC8I	GCNNGC	2 cut(s)	398, 772
BstDEI	CTNAG	6 cut(s)	171, 299, 923, 1150, 1242, 1566
BstDSI	CCRYGG	1 cut(s)	1027
BstEII	GGTNACC	1 cut(s)	1916
BstF5I	GGATG	4 cut(s)	381, 1402, 1740, 1900
BstFNI	CGCG	2 cut(s)	478, 1671
BstH2I	RGCGCY	2 cut(s)	71, 257
BstHHI	GCGC	2 cut(s)	70, 256
BstKTI	GATC	6 cut(s)	235, 239, 723, 1049, 1669, 1827
BstMAI	GTCTC	5 cut(s)	601, 636, 902, 1266, 2125
BstMBI	GATC	6 cut(s)	232, 236, 720, 1046, 1666, 1824
BstMCI	CGRYCG	1 cut(s)	239
BstMWI	GCNNNNNNNGC	4 cut(s)	313, 1442, 1445, 1588
BstNI	CCWGG	3 cut(s)	182, 373, 1063
BstNSI	RCATGY	1 cut(s)	865
BstPI	GGTNACC	1 cut(s)	1916
BstSCI	CCNGG	3 cut(s)	180, 371, 1061
BstSFI	CTRYAG	1 cut(s)	318
BstUI	CGCG	2 cut(s)	478, 1671
BstV1I	GCAGC	6 cut(s)	57, 295, 380, 1432, 1448, 1847
BstV2I	GAAGAC	1 cut(s)	531
BstX2I	RGATCY	1 cut(s)	720
BstXI	CCANNNNNNTGG	2 cut(s)	141, 1267
BstYI	RGATCY	1 cut(s)	720
BstZ17I	GTATAC	1 cut(s)	2005
Bsu15I	ATCGAT	1 cut(s)	235
BsuRI	GGCC	8 cut(s)	297, 316, 380, 427, 643, 1259, 1591, 1658
BsuTUI	ATCGAT	1 cut(s)	235
BtgI	CCRYGG	1 cut(s)	1027
BtgZI	GCGATG	1 cut(s)	638
BtsCI	GGATG	4 cut(s)	381, 1402, 1740, 1900
BtsI	GCAGTG	2 cut(s)	704, 1398
BtsIMutI	CAGTG	3 cut(s)	704, 1398, 1429
BtuMI	TCGCGA	2 cut(s)	478, 1671
Cac8I	GCNNGC	2 cut(s)	398, 772
CaiI	CAGNNNCTG	1 cut(s)	1442
CciI	TCATGA	1 cut(s)	1560
CfoI	GCGC	2 cut(s)	70, 256
Cfr10I	RCCGGY	1 cut(s)	547
ClaI	ATCGAT	1 cut(s)	235
CsiI	ACCWGGT	1 cut(s)	180
Csp6I	GTAC	4 cut(s)	105, 853, 1531, 1802
CviAII	CATG	7 cut(s)	264, 862, 1008, 1028, 1315, 1360, 1561
CviQI	GTAC	4 cut(s)	105, 853, 1531, 1802
DdeI	CTNAG	6 cut(s)	171, 299, 923, 1150, 1242, 1566
DinI	GGCGCC	1 cut(s)	255
DpnI	GATC	6 cut(s)	234, 238, 722, 1048, 1668, 1826
DpnII	GATC	6 cut(s)	232, 236, 720, 1046, 1666, 1824
EaeI	YGGCCR	1 cut(s)	1589
Eam1104I	CTCTTC	2 cut(s)	1140, 1566
EarI	CTCTTC	2 cut(s)	1140, 1566
Ecl136II	GAGCTC	1 cut(s)	664
Eco130I	CCWWGG	2 cut(s)	1027, 1307
Eco24I	GRGCYC	1 cut(s)	666
Eco31I	GGTCTC	1 cut(s)	1266
Eco47I	GGWCC	7 cut(s)	167, 351, 369, 388, 441, 1311, 1546
Eco53kI	GAGCTC	1 cut(s)	664
Eco57I	CTGAAG	1 cut(s)	1329
Eco88I	CYCGRG	1 cut(s)	972
Eco91I	GGTNACC	1 cut(s)	1916
EcoICRI	GAGCTC	1 cut(s)	664
EcoO109I	RGGNCCY	1 cut(s)	369
EcoO65I	GGTNACC	1 cut(s)	1916
EcoRI	GAATTC	1 cut(s)	2014
EcoRII	CCWGG	3 cut(s)	180, 371, 1061
EcoT14I	CCWWGG	2 cut(s)	1027, 1307
EcoT38I	GRGCYC	1 cut(s)	666
EgeI	GGCGCC	1 cut(s)	255
EheI	GGCGCC	1 cut(s)	255
ErhI	CCWWGG	2 cut(s)	1027, 1307
Esp3I	CGTCTC	1 cut(s)	902
FaeI	CATG	7 cut(s)	267, 865, 1011, 1031, 1318, 1363, 1564
FalI	AAGNNNNNCTT	5 cut(s)	404, 436, 707, 739, 2118
FaqI	GGGAC	2 cut(s)	601, 1348
FatI	CATG	7 cut(s)	263, 861, 1007, 1027, 1314, 1359, 1560
FauI	CCCGC	2 cut(s)	389, 1497
FauNDI	CATATG	2 cut(s)	136, 983
FblI	GTMKAC	1 cut(s)	2004
Fnu4HI	GCNGC	9 cut(s)	71, 257, 284, 394, 577, 1437, 1446, 1592, 1836
FokI	GGATG	4 cut(s)	388, 1409, 1727, 1887
FriOI	GRGCYC	1 cut(s)	666
Fsp4HI	GCNGC	9 cut(s)	71, 257, 284, 394, 577, 1437, 1446, 1592, 1836
GlaI	GCGC	2 cut(s)	69, 255
GluI	GCNGC	9 cut(s)	71, 257, 284, 394, 577, 1437, 1446, 1592, 1836
GsaI	CCCAGC	1 cut(s)	623
GsuI	CTGGAG	1 cut(s)	583
HaeII	RGCGCY	2 cut(s)	71, 257
HaeIII	GGCC	8 cut(s)	297, 316, 380, 427, 643, 1259, 1591, 1658
HapII	CCGG	4 cut(s)	5, 74, 548, 635
HhaI	GCGC	2 cut(s)	70, 256
Hin1I	GRCGYC	1 cut(s)	254
Hin1II	CATG	7 cut(s)	267, 865, 1011, 1031, 1318, 1363, 1564
Hin6I	GCGC	2 cut(s)	68, 254
HinP1I	GCGC	2 cut(s)	68, 254
HindIII	AAGCTT	2 cut(s)	405, 1580
HinfI	GANTC	8 cut(s)	50, 796, 1087, 1188, 1196, 1541, 1612, 1634
HpaII	CCGG	4 cut(s)	5, 74, 548, 635
HphI	GGTGA	6 cut(s)	22, 497, 859, 1296, 1588, 1928
Hpy166II	GTNNAC	3 cut(s)	488, 1086, 2005
Hpy188I	TCNGA	5 cut(s)	232, 1540, 1546, 2013, 2062
Hpy8I	GTNNAC	3 cut(s)	488, 1086, 2005
Hpy99I	CGWCG	1 cut(s)	202
HpyCH4III	ACNGT	5 cut(s)	595, 739, 857, 1759, 2077
HpyCH4IV	ACGT	3 cut(s)	1602, 1647, 1938
HpyCH4V	TGCA	7 cut(s)	557, 750, 1059, 1072, 1334, 1391, 1860
HpyF10VI	GCNNNNNNNGC	4 cut(s)	313, 1442, 1445, 1588
HpyF3I	CTNAG	6 cut(s)	171, 299, 923, 1150, 1242, 1566
HpySE526I	ACGT	3 cut(s)	1602, 1647, 1938
Hsp92I	GRCGYC	1 cut(s)	254
Hsp92II	CATG	7 cut(s)	267, 865, 1011, 1031, 1318, 1363, 1564
HspAI	GCGC	2 cut(s)	68, 254
KasI	GGCGCC	1 cut(s)	253
Kpn2I	TCCGGA	1 cut(s)	634
KpnI	GGTACC	1 cut(s)	108
Kzo9I	GATC	6 cut(s)	232, 236, 720, 1046, 1666, 1824
LmnI	GCTCC	1 cut(s)	332
Lsp1109I	GCAGC	6 cut(s)	57, 295, 380, 1432, 1448, 1847
LweI	GCATC	5 cut(s)	615, 1321, 1378, 1749, 1847
MabI	ACCWGGT	1 cut(s)	180
MaeII	ACGT	3 cut(s)	1602, 1647, 1938
MaeIII	GTNAC	5 cut(s)	20, 516, 1222, 1732, 1916
MalI	GATC	6 cut(s)	234, 238, 722, 1048, 1668, 1826
MboI	GATC	6 cut(s)	232, 236, 720, 1046, 1666, 1824
MflI	RGATCY	1 cut(s)	720
MhlI	GDGCHC	2 cut(s)	666, 973
MluCI	AATT	8 cut(s)	680, 874, 894, 1620, 1718, 1785, 2014, 2044
Mly113I	GGCGCC	1 cut(s)	254
MlyI	GAGTC	2 cut(s)	1081, 1606
MmeI	TCCRAC	3 cut(s)	111, 299, 1761
MnlI	CCTC	7 cut(s)	94, 308, 633, 810, 955, 1567, 1948
MroI	TCCGGA	1 cut(s)	634
MseI	TTAA	7 cut(s)	711, 729, 893, 1127, 1143, 1962, 2136
MslI	CAYNNNNRTG	3 cut(s)	268, 1117, 1265
MspA1I	CMGCKG	4 cut(s)	393, 1594, 1709, 1923
MspCI	CTTAAG	2 cut(s)	1142, 2135
MspI	CCGG	4 cut(s)	5, 74, 548, 635
MspR9I	CCNGG	3 cut(s)	182, 373, 1063
MvaI	CCWGG	3 cut(s)	182, 373, 1063
MvnI	CGCG	2 cut(s)	478, 1671
MwoI	GCNNNNNNNGC	4 cut(s)	313, 1442, 1445, 1588
NarI	GGCGCC	1 cut(s)	254
NcoI	CCATGG	1 cut(s)	1027
NdeI	CATATG	2 cut(s)	136, 983
NdeII	GATC	6 cut(s)	232, 236, 720, 1046, 1666, 1824
NheI	GCTAGC	1 cut(s)	770
NlaIII	CATG	7 cut(s)	267, 865, 1011, 1031, 1318, 1363, 1564
NlaIV	GGNNCC	6 cut(s)	106, 255, 334, 352, 370, 442
NmuCI	GTSAC	3 cut(s)	1222, 1732, 1916
NruI	TCGCGA	2 cut(s)	478, 1671
NspI	RCATGY	1 cut(s)	865
PagI	TCATGA	1 cut(s)	1560
PfeI	GAWTC	6 cut(s)	50, 796, 1188, 1196, 1541, 1634
PfoI	TCCNGGA	1 cut(s)	371
PkrI	GCNGC	9 cut(s)	72, 258, 285, 395, 578, 1438, 1447, 1593, 1837
Ple19I	CGATCG	1 cut(s)	239
PleI	GAGTC	2 cut(s)	1081, 1606
PluTI	GGCGCC	1 cut(s)	257
PpsI	GAGTC	2 cut(s)	1081, 1606
Ppu21I	YACGTR	1 cut(s)	1648
PpuMI	RGGWCCY	1 cut(s)	369
Psp124BI	GAGCTC	1 cut(s)	666
Psp5II	RGGWCCY	1 cut(s)	369
Psp6I	CCWGG	3 cut(s)	180, 371, 1061
PspEI	GGTNACC	1 cut(s)	1916
PspFI	CCCAGC	1 cut(s)	619
PspGI	CCWGG	3 cut(s)	180, 371, 1061
PspN4I	GGNNCC	6 cut(s)	106, 255, 334, 352, 370, 442
PspPPI	RGGWCCY	1 cut(s)	369
PstNI	CAGNNNCTG	1 cut(s)	1442
PsuI	RGATCY	1 cut(s)	720
PvuI	CGATCG	1 cut(s)	239
PvuII	CAGCTG	1 cut(s)	1709
RruI	TCGCGA	2 cut(s)	478, 1671
RsaI	GTAC	4 cut(s)	106, 854, 1532, 1803
RsaNI	GTAC	4 cut(s)	105, 853, 1531, 1802
RseI	CAYNNNNRTG	3 cut(s)	268, 1117, 1265
SacI	GAGCTC	1 cut(s)	666
SaqAI	TTAA	7 cut(s)	711, 729, 893, 1127, 1143, 1962, 2136
SatI	GCNGC	9 cut(s)	71, 257, 284, 394, 577, 1437, 1446, 1592, 1836
Sau3AI	GATC	6 cut(s)	232, 236, 720, 1046, 1666, 1824
ScaI	AGTACT	1 cut(s)	1803
SchI	GAGTC	2 cut(s)	1081, 1606
ScrFI	CCNGG	3 cut(s)	182, 373, 1063
SduI	GDGCHC	2 cut(s)	666, 973
SexAI	ACCWGGT	1 cut(s)	180
SfaNI	GCATC	5 cut(s)	615, 1321, 1378, 1749, 1847
SfcI	CTRYAG	1 cut(s)	318
SfoI	GGCGCC	1 cut(s)	255
SinI	GGWCC	7 cut(s)	167, 351, 369, 388, 441, 1311, 1546
SmiMI	CAYNNNNRTG	3 cut(s)	268, 1117, 1265
SmlI	CTYRAG	2 cut(s)	1142, 2135
SmoI	CTYRAG	2 cut(s)	1142, 2135
SpeI	ACTAGT	2 cut(s)	530, 1729
Sse9I	AATT	8 cut(s)	680, 874, 894, 1620, 1718, 1785, 2014, 2044
SsiI	CCGC	8 cut(s)	257, 391, 396, 576, 886, 1490, 1592, 1921
SspDI	GGCGCC	1 cut(s)	253
SstI	GAGCTC	1 cut(s)	666
StyD4I	CCNGG	3 cut(s)	180, 371, 1061
StyI	CCWWGG	2 cut(s)	1027, 1307
TaaI	ACNGT	5 cut(s)	595, 739, 857, 1759, 2077
TaiI	ACGT	3 cut(s)	1605, 1650, 1941
TaqI	TCGA	5 cut(s)	197, 235, 239, 1615, 2087
TasI	AATT	8 cut(s)	680, 874, 894, 1620, 1718, 1785, 2014, 2044
TatI	WGTACW	2 cut(s)	852, 1801
TauI	GCSGC	3 cut(s)	259, 579, 1594
TfiI	GAWTC	6 cut(s)	50, 796, 1188, 1196, 1541, 1634
Tru1I	TTAA	7 cut(s)	711, 729, 893, 1127, 1143, 1962, 2136
Tru9I	TTAA	7 cut(s)	711, 729, 893, 1127, 1143, 1962, 2136
TscAI	CASTG	3 cut(s)	704, 1398, 1429
TseFI	GTSAC	3 cut(s)	1222, 1732, 1916
TseI	GCWGC	6 cut(s)	70, 283, 393, 1436, 1445, 1835
Tsp45I	GTSAC	3 cut(s)	1222, 1732, 1916
TspDTI	ATGAA	7 cut(s)	554, 970, 1331, 1413, 1549, 1568, 2081
TspGWI	ACGGA	3 cut(s)	1241, 1893, 1932
TspRI	CASTG	3 cut(s)	704, 1398, 1429
Vha464I	CTTAAG	2 cut(s)	1142, 2135
VpaK11BI	GGWCC	7 cut(s)	167, 351, 369, 388, 441, 1311, 1546
XapI	RAATTY	1 cut(s)	2014
XceI	RCATGY	1 cut(s)	865
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1321
XmiI	GTMKAC	1 cut(s)	2004
ZrmI	AGTACT	1 cut(s)	1803

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.